##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931113_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2790026 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.945082232208588 31.0 28.0 33.0 23.0 34.0 2 28.686158480243552 31.0 28.0 33.0 20.0 34.0 3 28.71577469170538 31.0 28.0 34.0 20.0 34.0 4 31.94180269287813 35.0 32.0 37.0 19.0 37.0 5 32.01543498160949 35.0 32.0 37.0 20.0 37.0 6 32.29663666216731 35.0 32.0 37.0 23.0 37.0 7 32.03846559136008 35.0 32.0 37.0 19.0 37.0 8 32.16398270123648 35.0 32.0 37.0 22.0 37.0 9 33.195350509278406 37.0 32.0 39.0 19.0 39.0 10-11 33.21063585070533 37.0 32.0 39.0 21.0 39.0 12-13 33.25760835203686 37.0 32.0 39.0 21.5 39.0 14-15 34.039716117340845 38.0 32.0 40.0 20.0 41.0 16-17 34.02745906310551 38.0 32.0 40.0 20.5 41.0 18-19 34.0547165151866 38.0 32.0 40.0 20.5 41.0 20-21 34.26803388212153 38.0 32.0 40.0 24.0 41.0 22-23 34.18378681775725 38.0 32.0 40.0 23.0 41.0 24-25 34.058167199875555 38.0 32.0 40.0 21.5 41.0 26-27 33.71933594884062 38.0 32.0 40.0 18.5 41.0 28-29 33.6612601459628 38.0 32.0 40.0 19.0 41.0 30-31 33.39688071007224 37.5 31.5 40.0 18.0 41.0 32-33 33.21706195569504 37.0 31.0 40.0 17.5 41.0 34-35 33.200295445275415 37.0 31.0 40.0 18.0 41.0 36-37 32.84391632909514 37.0 30.5 40.0 16.0 41.0 38-39 32.68454612967764 37.0 30.0 40.0 15.5 41.0 40-41 32.576431904218815 37.0 30.0 40.0 15.0 41.0 42-43 32.766619199964445 37.0 30.5 40.0 15.0 41.0 44-45 32.82647455615109 37.0 31.0 40.0 14.5 41.0 46-47 32.60365082619302 37.0 30.5 40.0 11.0 41.0 48-49 32.40390680947059 37.0 30.0 40.0 9.0 41.0 50-51 31.91050764401479 36.5 30.0 39.5 8.0 40.5 52-53 31.84304698235787 36.5 30.0 39.5 8.0 40.5 54-55 31.85457053088394 36.5 30.0 40.0 7.5 41.0 56-57 31.61681683253131 36.0 30.0 40.0 6.0 41.0 58-59 31.4773505694929 36.0 30.0 40.0 2.0 41.0 60-61 31.329212165047924 36.0 29.0 40.0 2.0 41.0 62-63 31.184537886026867 35.5 29.5 39.0 2.0 41.0 64-65 30.853532368515562 35.0 29.0 39.0 2.0 41.0 66-67 30.65868508035409 35.0 29.0 39.0 2.0 40.0 68-69 30.464619505337943 35.0 29.0 39.0 2.0 40.0 70-71 30.16732137980076 35.0 29.0 38.0 2.0 40.0 72-73 29.840602023063582 34.0 29.0 37.5 2.0 40.0 74-75 29.42154929739006 34.0 28.0 37.0 2.0 39.5 76-77 29.117756250300175 34.0 28.0 36.5 2.0 39.0 78-79 28.702404923825085 34.0 28.0 36.0 2.0 39.0 80-81 28.288440322778353 34.0 27.0 36.0 2.0 38.5 82-83 27.945413411918025 33.0 27.0 35.0 2.0 37.0 84-85 27.614484596200896 33.0 26.0 35.0 2.0 37.0 86-87 27.289505725036253 33.0 26.0 35.0 2.0 36.0 88-89 26.94204677662502 33.0 26.0 35.0 2.0 36.0 90-91 26.558474365471863 33.0 25.0 35.0 2.0 36.0 92-93 26.204552036432634 32.5 25.0 35.0 2.0 35.0 94-95 25.90993865290144 32.0 24.0 35.0 2.0 35.0 96-97 25.524379880330862 32.0 22.5 35.0 2.0 35.0 98-99 25.17093424935825 32.0 19.5 35.0 2.0 35.0 100-101 24.587474632852885 31.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.058910775154732 1101 2 5.711193569831533 1101 3 5.2516954656399975 1101 4 6.585360942221733 1101 5 6.01326622189389 1101 6 5.496764480142776 1101 7 6.103115185113747 1101 8 5.330186065380495 1101 9 6.168972311036491 1101 10-11 6.179231299361042 1101 12-13 6.021049053315608 1101 14-15 6.297814746418027 1101 16-17 6.247333622944499 1101 18-19 6.260945255083612 1101 20-21 5.797602313294149 1101 22-23 5.712686229390208 1101 24-25 5.643894846694661 1101 26-27 5.828954752671965 1101 28-29 5.626365922496998 1101 30-31 5.804131860483828 1101 32-33 5.670852418052373 1101 34-35 5.408115887925529 1101 36-37 5.611436526107369 1101 38-39 5.452131027355502 1101 40-41 5.587644522380074 1101 42-43 5.575332951529376 1101 44-45 5.499854009982229 1101 46-47 5.445072691946777 1101 48-49 5.290260660676363 1101 50-51 5.274968983230028 1101 52-53 4.977396374654983 1101 54-55 5.140034897722984 1101 56-57 5.159275874970639 1101 58-59 5.012143374725181 1101 60-61 4.8653072730203455 1101 62-63 4.853322476305628 1101 64-65 4.783773311226376 1101 66-67 4.762564771551084 1101 68-69 4.764813027835796 1101 70-71 4.675160386099112 1101 72-73 4.431477770947524 1101 74-75 4.690212710727586 1101 76-77 4.519884782689449 1101 78-79 4.435555375502233 1101 80-81 4.286011483862573 1101 82-83 4.212340829467436 1101 84-85 4.14542715167363 1101 86-87 4.052685784624295 1101 88-89 4.109870271524812 1101 90-91 4.1713871215795315 1101 92-93 4.070034224377334 1101 94-95 4.079165603109111 1101 96-97 4.029219533894022 1101 98-99 3.7157347247594004 1101 100-101 3.8129268049098677 1102 1 5.276016593960112 1102 2 5.849827870893844 1102 3 5.511793782197103 1102 4 6.779676906539159 1102 5 6.464129611510689 1102 6 5.937531609306273 1102 7 6.385229391241001 1102 8 5.897605946132799 1102 9 6.744779909067567 1102 10-11 6.708824524530662 1102 12-13 6.686713053057488 1102 14-15 6.942211950559717 1102 16-17 6.855518015282005 1102 18-19 6.89786504236227 1102 20-21 6.194933696175788 1102 22-23 6.268264771131804 1102 24-25 6.140814486882814 1102 26-27 6.291740776480378 1102 28-29 6.157537286411863 1102 30-31 6.34908236215734 1102 32-33 6.135858757178536 1102 34-35 6.159471572056116 1102 36-37 6.260651853633632 1102 38-39 6.063722067361272 1102 40-41 6.256784642374285 1102 42-43 6.189436036349349 1102 44-45 6.18031891244857 1102 46-47 6.1228963709238045 1102 48-49 5.820574036200949 1102 50-51 5.87151784463882 1102 52-53 5.65364919033097 1102 54-55 6.027134949948014 1102 56-57 5.795178325814732 1102 58-59 5.918952051736266 1102 60-61 5.712723572988811 1102 62-63 5.522831864633723 1102 64-65 5.627015799567474 1102 66-67 5.619641596129682 1102 68-69 5.622021443057417 1102 70-71 5.466841050087783 1102 72-73 5.37219776788551 1102 74-75 5.415950250975818 1102 76-77 5.243932284697312 1102 78-79 5.183586814278261 1102 80-81 5.202928995192497 1102 82-83 5.196245490781273 1102 84-85 5.106128681636285 1102 86-87 5.132987274287309 1102 88-89 5.1836665255567524 1102 90-91 5.071379221396093 1102 92-93 5.002996067203064 1102 94-95 4.997219823515213 1102 96-97 4.973501730660626 1102 98-99 4.754333882262962 1102 100-101 4.596624922689184 1103 1 4.880638121811245 1103 2 5.512508082400451 1103 3 5.148249930270648 1103 4 6.4957393917386455 1103 5 5.994164048025443 1103 6 5.387344827239492 1103 7 5.976326759138448 1103 8 5.401998328161483 1103 9 6.02346389247775 1103 10-11 6.157087413470769 1103 12-13 6.281734625936863 1103 14-15 6.436439908557318 1103 16-17 6.331975110903631 1103 18-19 6.217455867132728 1103 20-21 5.647099151000447 1103 22-23 5.75547358140556 1103 24-25 5.559442178684055 1103 26-27 5.776181280077754 1103 28-29 5.486891325063635 1103 30-31 5.764385476642268 1103 32-33 5.558244123567473 1103 34-35 5.525119658686915 1103 36-37 5.503737432834626 1103 38-39 5.379317975782403 1103 40-41 5.49024239986062 1103 42-43 5.43840059174434 1103 44-45 5.060537758218331 1103 46-47 5.101538187655713 1103 48-49 4.8958054917704 1103 50-51 4.827038344944217 1103 52-53 4.551361177019256 1103 54-55 4.826613514707489 1103 56-57 4.909936568615969 1103 58-59 4.92815075714277 1103 60-61 4.763260837485273 1103 62-63 4.88564928930208 1103 64-65 4.7691354000017405 1103 66-67 4.836339931819012 1103 68-69 4.771911726133073 1103 70-71 4.640708438446595 1103 72-73 4.3985611256175545 1103 74-75 4.398515957365824 1103 76-77 4.5263469036773465 1103 78-79 4.516428216170144 1103 80-81 4.567541400725421 1103 82-83 4.516043838042986 1103 84-85 4.497284258311531 1103 86-87 4.470158899999195 1103 88-89 4.478592996770999 1103 90-91 4.524658518211357 1103 92-93 4.409175483935975 1103 94-95 4.475471387600209 1103 96-97 4.336945624935069 1103 98-99 4.2867520880514896 1103 100-101 4.3620220273227766 1104 1 4.551535729506188 1104 2 5.43272156906999 1104 3 5.052165011138651 1104 4 6.396995885947703 1104 5 6.049175637288602 1104 6 5.563605054835367 1104 7 6.121582035901984 1104 8 5.536872251957945 1104 9 6.38759029764995 1104 10-11 6.271819657736849 1104 12-13 6.245069999111749 1104 14-15 6.32148996669973 1104 16-17 6.304552865700938 1104 18-19 6.414711531768624 1104 20-21 5.673114128533221 1104 22-23 5.852918822117893 1104 24-25 5.610151837459423 1104 26-27 5.848431965582943 1104 28-29 5.784259583416766 1104 30-31 5.973287539486513 1104 32-33 5.8750158428432435 1104 34-35 5.6484468053381995 1104 36-37 5.89218902545668 1104 38-39 5.593682587181821 1104 40-41 5.847271832790312 1104 42-43 5.807476445090444 1104 44-45 5.78163515401377 1104 46-47 5.773077353459463 1104 48-49 5.521000369948187 1104 50-51 5.440414074069661 1104 52-53 5.295682968663524 1104 54-55 5.627170070019517 1104 56-57 5.551107420238793 1104 58-59 5.577469158364284 1104 60-61 5.346410897796201 1104 62-63 5.345059607967983 1104 64-65 5.476006015963662 1104 66-67 5.417554398319957 1104 68-69 5.40017567127482 1104 70-71 5.306602461936404 1104 72-73 5.23393722262194 1104 74-75 5.180209489294313 1104 76-77 5.11631621992851 1104 78-79 5.0257753710064215 1104 80-81 5.0243776818322985 1104 82-83 4.83011167787231 1104 84-85 4.849120976966727 1104 86-87 4.7440892942894415 1104 88-89 4.803034538239125 1104 90-91 4.854263514351857 1104 92-93 4.762295818644294 1104 94-95 4.726902916517076 1104 96-97 4.660998332820888 1104 98-99 4.536641086118124 1104 100-101 4.646906970569912 1105 1 4.281781573004359 1105 2 4.924820367740587 1105 3 4.655658953186968 1105 4 5.957083536914041 1105 5 3.5242597369363935 1105 6 4.729318286215186 1105 7 5.326354970383104 1105 8 0.8850234717617376 1105 9 0.45294227826902755 1105 10-11 2.0140428349266983 1105 12-13 5.221739413010042 1105 14-15 3.539615835407787 1105 16-17 5.5735700471166005 1105 18-19 5.955307856754047 1105 20-21 3.07454644661464 1105 22-23 5.086936476572006 1105 24-25 5.13523339540804 1105 26-27 5.3968722095295725 1105 28-29 2.9315215379928325 1105 30-31 5.2724470607621825 1105 32-33 5.367415644590704 1105 34-35 5.18423767150691 1105 36-37 5.427721744420538 1105 38-39 5.386284242204521 1105 40-41 5.465397466155856 1105 42-43 2.8563730417081956 1105 44-45 4.892417300742302 1105 46-47 5.230859918626976 1105 48-49 5.045337916069439 1105 50-51 5.1283407441660565 1105 52-53 5.033452076946016 1105 54-55 5.289960323827003 1105 56-57 5.056785293759134 1105 58-59 5.137883158796367 1105 60-61 5.013142832787064 1105 62-63 4.809232146038163 1105 64-65 4.731890967363075 1105 66-67 4.858350387895225 1105 68-69 4.922052758468748 1105 70-71 4.687071457207431 1105 72-73 4.6210185424039345 1105 74-75 4.806730660942321 1105 76-77 4.632408140949444 1105 78-79 4.718322167688843 1105 80-81 4.626736331587566 1105 82-83 4.465326660906467 1105 84-85 4.419161827720508 1105 86-87 4.42035874358325 1105 88-89 4.429410621226545 1105 90-91 4.3783522563660995 1105 92-93 4.335265271656514 1105 94-95 4.29272377002907 1105 96-97 4.198561587005397 1105 98-99 4.214490883935369 1105 100-101 4.365923563629284 1106 1 4.320990667453774 1106 2 4.571432041690173 1106 3 4.628876891966428 1106 4 6.118723705707929 1106 5 6.046809895776871 1106 6 5.113544202922082 1106 7 5.915139747694571 1106 8 5.464804790276595 1106 9 6.0812431385392 1106 10-11 5.959355586349865 1106 12-13 6.193363758032213 1106 14-15 5.854499746369058 1106 16-17 5.95067330102707 1106 18-19 6.246287389630439 1106 20-21 5.548874355628207 1106 22-23 5.621134744919907 1106 24-25 5.700024874367248 1106 26-27 5.771469185559102 1106 28-29 5.8033384256629255 1106 30-31 5.9274082508523875 1106 32-33 5.76504965264883 1106 34-35 5.2970683445701745 1106 36-37 5.465828783142385 1106 38-39 5.3692638861984925 1106 40-41 5.455511382636324 1106 42-43 2.430684781017977 1106 44-45 4.796691763250337 1106 46-47 5.010034729111233 1106 48-49 4.745021335807163 1106 50-51 4.941846325465594 1106 52-53 4.7443956381280366 1106 54-55 5.012174928555055 1106 56-57 4.968378683102017 1106 58-59 4.992010521551752 1106 60-61 4.7059216055563375 1106 62-63 4.827697240181482 1106 64-65 4.939846540250176 1106 66-67 4.945129519175925 1106 68-69 4.914204555559671 1106 70-71 4.648000761794854 1106 72-73 4.559100134152722 1106 74-75 4.727894130095926 1106 76-77 4.7100226405351115 1106 78-79 4.8636623054012595 1106 80-81 4.788759672568094 1106 82-83 4.71075760578848 1106 84-85 4.761764777892132 1106 86-87 4.858121291614083 1106 88-89 4.968602445279199 1106 90-91 4.896026292958375 1106 92-93 4.927036281395548 1106 94-95 4.848520438493534 1106 96-97 4.924829467369509 1106 98-99 4.841714978209197 1106 100-101 4.846511454224601 1107 1 3.1912334069422883 1107 2 0.27739299664332506 1107 3 1.3622552388038116 1107 4 3.089996754289171 1107 5 3.01629032323844 1107 6 4.21533974158368 1107 7 5.1070935838454155 1107 8 4.352034292460587 1107 9 4.894643825411926 1107 10-11 2.750542734160881 1107 12-13 4.995286834955294 1107 14-15 3.638844007980879 1107 16-17 2.1837971965290564 1107 18-19 2.7194580908407957 1107 20-21 2.5485104964704632 1107 22-23 4.334791133503138 1107 24-25 4.270374312532031 1107 26-27 3.479061463242406 1107 28-29 4.2359875954138495 1107 30-31 3.835459416201992 1107 32-33 4.330300686292258 1107 34-35 4.761241554866597 1107 36-37 4.872270507326306 1107 38-39 4.908049271814175 1107 40-41 3.5244518564451326 1107 42-43 3.9850883937684642 1107 44-45 4.384412037665996 1107 46-47 4.628093896861557 1107 48-49 2.5913014698473766 1107 50-51 4.14136201350534 1107 52-53 4.1159914918841665 1107 54-55 4.7117935641457365 1107 56-57 3.455322057781089 1107 58-59 2.8604533460657713 1107 60-61 3.870270076764367 1107 62-63 4.4589230662626385 1107 64-65 4.567287480865694 1107 66-67 4.603822126958256 1107 68-69 4.586625240098368 1107 70-71 4.245297242405705 1107 72-73 4.1948779508310565 1107 74-75 4.329171596669227 1107 76-77 4.272671643569353 1107 78-79 4.243975982837821 1107 80-81 4.342378674935471 1107 82-83 4.080995552172396 1107 84-85 4.106046187314959 1107 86-87 4.118563174178853 1107 88-89 4.254746830261361 1107 90-91 4.2828175386654195 1107 92-93 4.241651499294253 1107 94-95 4.351433812505739 1107 96-97 4.298561587005398 1107 98-99 4.247795343985597 1107 100-101 4.394626513742455 1108 1 -1.975260475564486 1108 2 -1.111590306874863 1108 3 -1.9904381824662423 1108 4 -4.123781076174005 1108 5 -9.007663721922526 1108 6 -7.28872984624887 1108 7 -5.745511020618952 1108 8 -5.835383715887655 1108 9 -7.572859102219887 1108 10-11 -3.109900784098201 1108 12-13 -1.6531852286120383 1108 14-15 -6.5599584918215506 1108 16-17 -5.97515251106698 1108 18-19 -4.239455290771517 1108 20-21 -4.497017501029372 1108 22-23 -3.43928742581463 1108 24-25 -8.001145397794069 1108 26-27 -9.487288013376052 1108 28-29 -6.229587271136431 1108 30-31 -6.019194350923762 1108 32-33 -2.564564597741917 1108 34-35 -0.9171214050230496 1108 36-37 -4.574763379562931 1108 38-39 -5.694291845786818 1108 40-41 -3.4917513732895493 1108 42-43 -4.447039895776115 1108 44-45 -1.8362438865676083 1108 46-47 -2.3731774676663235 1108 48-49 -1.3130550438639972 1108 50-51 -0.6416119215910392 1108 52-53 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-5.216390731218443 2313 60-61 -3.6445457412204654 2313 62-63 -5.265672907012913 2313 64-65 -5.003105711622567 2313 66-67 -6.873931574739103 2313 68-69 -6.84717080540543 2313 70-71 -6.525190749649184 2313 72-73 -6.001451951660815 2313 74-75 -6.748956789029577 2313 76-77 -5.154411689763979 2313 78-79 -6.442648249737115 2313 80-81 -8.511598392597932 2313 82-83 -7.223426170471157 2313 84-85 -6.716238482240833 2313 86-87 -6.107813676669828 2313 88-89 -8.604315556882012 2313 90-91 -7.9932726563508325 2313 92-93 -6.464695557404919 2313 94-95 -7.868817180632579 2313 96-97 -7.238058131304463 2313 98-99 -6.996604710185583 2313 100-101 -8.02429051406542 2314 1 -14.655736290027411 2314 2 -16.587700243449916 2314 3 -15.458840332291759 2314 4 -25.206157091864675 2314 5 -20.45518403573592 2314 6 -18.928832752588818 2314 7 -18.353891264639433 2314 8 -14.792254751828457 2314 9 -17.777617246849147 2314 10-11 -16.089040599172453 2314 12-13 -16.99148472681627 2314 14-15 -19.268513218126344 2314 16-17 -19.717965623983762 2314 18-19 -17.91675985787715 2314 20-21 -18.65874474828478 2314 22-23 -18.89762436766236 2314 24-25 -19.691863449705732 2314 26-27 -18.93713900295806 2314 28-29 -20.58229620411995 2314 30-31 -20.26835760011503 2314 32-33 -17.730028567787 2314 34-35 -19.521407046532 2314 36-37 -18.785727744421944 2314 38-39 -18.70625725499235 2314 40-41 -20.927951989708713 2314 42-43 -20.351756011825938 2314 44-45 -17.858143323639364 2314 46-47 -17.441224284956625 2314 48-49 -17.4020843110384 2314 50-51 -16.85158670444338 2314 52-53 -16.91744382280115 2314 54-55 -16.816382426530254 2314 56-57 -15.774878991169958 2314 58-59 -15.28431646745404 2314 60-61 -15.55997467848039 2314 62-63 -18.39371388012431 2314 64-65 -16.27570494337673 2314 66-67 -17.090000716864584 2314 68-69 -18.82854084381772 2314 70-71 -18.24446091610245 2314 72-73 -17.14332134986824 2314 74-75 -16.066370361372726 2314 76-77 -16.290775326127616 2314 78-79 -15.768640567278728 2314 80-81 -16.24552925047245 2314 82-83 -16.642824377897533 2314 84-85 -16.491462553943776 2314 86-87 -16.949939156823476 2314 88-89 -16.311613892349364 2314 90-91 -15.773170223572343 2314 92-93 -15.751763419120667 2314 94-95 -15.29186455579263 2314 96-97 -15.001438412994602 2314 98-99 -14.776374236433984 2314 100-101 -14.1409358405699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84639.0 3 13843.0 4 9664.0 5 7792.0 6 8092.0 7 9152.0 8 10927.0 9 12504.0 10 13777.0 11 15108.0 12 16743.0 13 17991.0 14 20311.0 15 22453.0 16 24017.0 17 25415.0 18 23750.0 19 21663.0 20 21788.0 21 23504.0 22 26996.0 23 32974.0 24 40647.0 25 49689.0 26 59231.0 27 68118.0 28 76361.0 29 85326.0 30 97392.0 31 109239.0 32 126289.0 33 151969.0 34 184000.0 35 226985.0 36 280718.0 37 347988.0 38 332179.0 39 90647.0 40 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96963138580247 20.147421292887753 14.396671150605474 23.486276170704304 2 14.483446259665822 20.545073299717885 41.579568111280516 23.391912329335778 3 17.896858308847303 25.665352222524092 30.86648654887087 25.571302919757738 4 11.402440254981276 17.614509483954112 40.35552935138922 30.627520909675393 5 12.35793586752441 37.692757055513596 36.1631672622157 13.786139814746287 6 29.45751423893281 38.34509806172572 18.796144689656558 13.401243009684913 7 25.767183959102656 32.77090398683064 23.27310055412913 18.188811499937568 8 24.20085753753969 36.28442259736998 21.390789544500457 18.12393032058987 9 24.90583246682698 16.53351531760777 21.76875654364788 36.79189567191737 10-11 23.334431477171023 26.910891462395455 29.86263028489202 19.892046775541495 12-13 24.28387774577606 24.537400329981775 29.479222358277426 21.699499565964736 14-15 21.55948699616977 26.26488736139106 27.227182283322136 24.948443359117036 16-17 20.94380046653508 29.2994796339494 27.953651534182665 21.803068365332855 18-19 20.783446881173248 28.383459017838565 29.882606488914792 20.950487612073395 20-21 21.76142331061164 27.63611755093722 29.880627691375867 20.721831447075274 22-23 21.329285472999942 27.538671650966943 29.60953145173486 21.522511424298248 24-25 21.220592680426336 27.645629522262517 29.729689003777015 21.404088793534136 26-27 21.158456246228774 27.8164971525835 29.646380331399392 21.378666269788337 28-29 21.067766883413718 27.87653311881092 29.730359107359046 21.325340890416317 30-31 21.28224157929189 27.67478783145951 29.717699564753037 21.325271024495564 32-33 21.18416567699158 27.859718489761796 29.533082655510544 21.423033177736077 34-35 21.483483783151247 27.943247849503276 29.490162491546617 21.083105875798854 36-37 21.33682592446772 27.655414263969973 29.443785062443496 21.563974749118806 38-39 21.238081173183826 27.92520314290785 29.545878275574527 21.2908374083338 40-41 21.438694968564743 27.738484204150847 29.467656596811338 21.355164230473076 42-43 21.309277902939403 27.810978797393528 29.39337933875194 21.486363960915128 44-45 21.42988596628746 28.005218996602345 29.18406720839287 21.380827828717326 46-47 21.38928196855813 27.925515742549265 29.0501770158978 21.635025272994802 48-49 21.34541648392157 28.011975521574684 29.129792518169122 21.512815476334623 50-51 21.264053216496567 28.178624941573844 28.92547530999492 21.631846531934666 52-53 21.312128798517517 28.341349219574415 28.77204204290928 21.574479938998785 54-55 21.371246289633046 28.152016607919748 28.76854015069577 21.708196951751432 56-57 21.305010697398465 28.288524118588303 28.618813038240955 21.78765214577228 58-59 21.309521069545177 28.319528635996168 28.738290701320818 21.63265959313783 60-61 21.455410176404925 28.30807472057149 28.64025310607253 21.596261996951053 62-63 21.314209975104177 28.260130909174325 28.728137300512614 21.69752181520889 64-65 21.265267778866576 28.218285420996075 28.73765334086492 21.77879345927242 66-67 21.308242288781535 28.19142187205424 28.619123979489796 21.881211859674426 68-69 21.387273810351587 28.184593978694107 28.626238608529096 21.80189360242521 70-71 21.413062100496553 28.05411132369376 28.558336015506665 21.97449056030302 72-73 21.482129557215597 28.11516810237611 28.581292790819866 21.821409549588427 74-75 21.381700385587806 28.30781863681557 28.479877965294946 21.830603012301676 76-77 21.422954481427773 28.231457341257755 28.40233388506057 21.943254292253908 78-79 21.469083083813555 28.279360120658374 28.33995095386208 21.911605841665992 80-81 21.487667140019482 28.29246035700026 28.31002291734916 21.909849585631104 82-83 21.604888269858417 28.210185138059646 28.404789059313423 21.780137532768514 84-85 21.639887943696582 28.219235232933315 28.262783214206603 21.8780936091635 86-87 21.46395768354847 28.286349302838037 28.338373907626668 21.911319105986827 88-89 21.625909579337254 28.14158362681925 28.326115957342335 21.906390836501167 90-91 21.62734325773308 28.184110112235512 28.325255750304834 21.86329087972657 92-93 21.74655361634623 28.14792765372079 28.264431944361807 21.84108678557117 94-95 21.516914179294385 28.13808903572942 28.35280029648469 21.992196488491505 96-97 21.560318792728097 28.07199646168172 28.353176637063598 22.014508108526588 98-99 21.535014369041722 28.079666641099404 28.202049013163315 22.18326997669556 100-101 21.641805488551004 28.017713813419657 28.186780338247747 22.153700359781595 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 151.0 1 111.0 2 100.0 3 144.0 4 142.5 5 158.0 6 244.0 7 438.5 8 794.5 9 1105.5 10 1193.0 11 1205.0 12 1337.0 13 1546.5 14 1737.0 15 1951.5 16 2191.5 17 2471.0 18 2803.0 19 3237.0 20 3780.0 21 4599.0 22 5790.5 23 7517.5 24 9942.0 25 13300.5 26 17898.0 27 23557.5 28 29968.0 29 37052.0 30 44239.0 31 51938.5 32 60619.5 33 68661.0 34 76090.0 35 83037.0 36 90839.5 37 97555.0 38 100597.5 39 102382.5 40 104023.0 41 105144.0 42 105630.5 43 105734.0 44 105551.0 45 105501.0 46 104285.5 47 101422.0 48 98773.0 49 95553.5 50 91326.5 51 87337.5 52 82097.0 53 76400.0 54 71204.0 55 64823.5 56 57690.0 57 50623.5 58 44199.0 59 38314.5 60 31995.5 61 25760.0 62 20293.5 63 15568.5 64 11700.5 65 8689.0 66 6351.5 67 4852.5 68 3845.0 69 2935.0 70 2315.0 71 1824.5 72 1375.0 73 1079.5 74 856.5 75 646.0 76 478.0 77 332.0 78 246.0 79 205.5 80 155.0 81 108.0 82 86.5 83 71.5 84 61.0 85 48.5 86 38.0 87 29.0 88 19.0 89 14.5 90 15.0 91 11.5 92 8.0 93 5.5 94 7.5 95 7.5 96 3.0 97 0.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05573424763783563 2 0.5345111479247864 3 0.0 4 0.5460164170513107 5 0.23655693531171393 6 0.09311741180906558 7 0.10573378169235698 8 0.10666567264964556 9 0.10652230481006271 10-11 0.18005208553612045 12-13 0.2452306896064768 14-15 0.13512418880684265 16-17 0.12637875059228837 18-19 0.1819517094105933 20-21 0.11164770507514983 22-23 0.10302771372023058 24-25 0.09940767577076343 26-27 0.17768651618300332 28-29 0.10967639728088556 30-31 0.1276690611485341 32-33 0.1045151550559027 34-35 0.1490308692463798 36-37 0.13429982372924124 38-39 0.12994502560191196 40-41 0.10852945456422271 42-43 0.10619972717100126 44-45 0.09021421305751273 46-47 0.09191670615255915 48-49 0.095823479781192 50-51 0.08311750499816131 52-53 0.09105649911506201 54-55 0.30327674365758595 56-57 0.32171743202393094 58-59 0.3344054858270138 60-61 0.16037484955337333 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2790026.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.10488436672699 #Duplication Level Percentage of deduplicated Percentage of total 1 86.38280818190105 64.87770819774838 2 9.0475653316981 13.590326960751877 3 2.1806269712708226 4.913272095727838 4 0.8497428643308528 2.5527935826808035 5 0.4341187646606097 1.6302219810630731 6 0.23382692212756445 1.0536926368931043 7 0.163617790061733 0.860194664204811 8 0.1166869066858236 0.7011005306999877 9 0.09016915233030318 0.609492938329189 >10 0.4661976592217346 6.258345085883965 >50 0.02419735710930681 1.2359771046365786 >100 0.010102861322751575 1.1726196987984667 >500 1.9384987396129426E-4 0.10005722483927867 >1k 9.692493693344006E-5 0.24462881335035086 >5k 4.846246846672003E-5 0.19956848439219962 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5483 0.19652146610820115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4234 0.15175485819845408 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.5841959895714236E-5 3.5841959895714236E-5 0.0 0.0 0.0 3 3.5841959895714236E-5 3.5841959895714236E-5 0.0 0.0 0.0 4 3.5841959895714236E-5 3.5841959895714236E-5 0.0 0.0 0.0 5 3.5841959895714236E-5 3.5841959895714236E-5 0.0 0.0 0.0 6 7.168391979142847E-5 3.5841959895714236E-5 0.0 0.0 3.5841959895714236E-5 7 7.168391979142847E-5 3.5841959895714236E-5 0.0 0.0 1.075258796871427E-4 8 7.168391979142847E-5 3.5841959895714236E-5 0.0 0.0 1.075258796871427E-4 9 7.168391979142847E-5 1.075258796871427E-4 0.0 0.0 1.075258796871427E-4 10-11 7.168391979142847E-5 1.075258796871427E-4 0.0 1.7920979947857118E-5 1.075258796871427E-4 12-13 7.168391979142847E-5 1.075258796871427E-4 0.0 3.5841959895714236E-5 1.075258796871427E-4 14-15 7.168391979142847E-5 1.4336783958285694E-4 0.0 3.5841959895714236E-5 1.075258796871427E-4 16-17 7.168391979142847E-5 1.4336783958285694E-4 0.0 3.5841959895714236E-5 1.2544685963499983E-4 18-19 7.168391979142847E-5 1.4336783958285694E-4 0.0 3.5841959895714236E-5 2.1505175937428542E-4 20-21 7.168391979142847E-5 1.4336783958285694E-4 0.0 1.2544685963499983E-4 2.5089371926999965E-4 22-23 7.168391979142847E-5 1.7920979947857118E-4 0.0 1.6128881953071406E-4 2.5089371926999965E-4 24-25 7.168391979142847E-5 1.7920979947857118E-4 0.0 3.5841959895714236E-4 2.5089371926999965E-4 26-27 7.168391979142847E-5 1.7920979947857118E-4 0.0 7.52681157809999E-4 2.5089371926999965E-4 28-29 7.168391979142847E-5 1.7920979947857118E-4 0.0 0.0021863595536385683 2.5089371926999965E-4 30-31 7.168391979142847E-5 1.7920979947857118E-4 0.0 0.0069533402197685616 2.867356791657139E-4 32-33 8.960489973928559E-5 1.971307794264283E-4 0.0 0.02084209967935783 3.225776390614281E-4 34-35 1.075258796871427E-4 2.6881469921785677E-4 0.0 0.04130785877981065 3.5841959895714236E-4 36-37 1.075258796871427E-4 3.4049861900928524E-4 0.0 0.07482009128230346 3.5841959895714236E-4 38-39 1.075258796871427E-4 3.942615588528566E-4 0.0 0.12050066916939126 3.5841959895714236E-4 40-41 1.075258796871427E-4 4.301035187485708E-4 0.0 0.1698729689257376 3.5841959895714236E-4 42-43 1.075258796871427E-4 4.480244986964279E-4 0.0 0.22297283251123826 3.5841959895714236E-4 44-45 1.075258796871427E-4 4.65945478644285E-4 0.0 0.2895492730175274 3.5841959895714236E-4 46-47 1.075258796871427E-4 4.65945478644285E-4 0.0 0.36555214897638943 3.5841959895714236E-4 48-49 1.075258796871427E-4 4.65945478644285E-4 0.0 0.44562308738341505 3.5841959895714236E-4 50-51 1.2544685963499983E-4 5.376293984357135E-4 0.0 0.5261420502891371 3.942615588528566E-4 52-53 1.4336783958285694E-4 5.376293984357135E-4 0.0 0.6046001005008556 4.480244986964279E-4 54-55 1.4336783958285694E-4 5.376293984357135E-4 0.0 0.696713937432841 4.65945478644285E-4 56-57 1.4336783958285694E-4 5.376293984357135E-4 0.0 0.8043115010397752 4.8386645859214213E-4 58-59 1.4336783958285694E-4 5.376293984357135E-4 0.0 0.9251347478482279 5.017874385399993E-4 60-61 1.4336783958285694E-4 5.734713583314278E-4 0.0 1.0480726702905279 5.197084184878564E-4 62-63 2.150517593742854E-4 5.734713583314278E-4 0.0 1.172336745248969 5.376293984357135E-4 64-65 2.150517593742854E-4 6.272342981749992E-4 0.0 1.3073175662162289 5.376293984357135E-4 66-67 2.150517593742854E-4 6.809972380185705E-4 0.0 1.460559865750355 5.376293984357135E-4 68-69 2.150517593742854E-4 6.809972380185705E-4 0.0 1.6343037663448299 5.376293984357135E-4 70-71 2.3297273932214253E-4 6.809972380185705E-4 0.0 1.8180834157101045 5.376293984357135E-4 72-73 2.5089371926999965E-4 6.809972380185705E-4 0.0 2.0056443918443767 5.376293984357135E-4 74-75 2.5089371926999965E-4 6.989182179664277E-4 0.0 2.200463364857532 5.376293984357135E-4 76-77 2.5089371926999965E-4 7.168391979142847E-4 0.0 2.41114240512454 5.376293984357135E-4 78-79 2.5089371926999965E-4 7.52681157809999E-4 0.0 2.646086452240947 5.376293984357135E-4 80-81 2.5089371926999965E-4 8.064440976535703E-4 0.0 2.8974998799294345 5.376293984357135E-4 82-83 2.5089371926999965E-4 8.602070374971416E-4 0.0 3.1581426122910683 5.376293984357135E-4 84-85 2.5089371926999965E-4 8.602070374971416E-4 0.0 3.418767423672754 5.734713583314278E-4 86-87 2.5089371926999965E-4 8.602070374971416E-4 0.0 3.6965426128645396 5.734713583314278E-4 88-89 2.5089371926999965E-4 9.3189095728857E-4 0.0 4.000643721599727 5.734713583314278E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3240 0.0 50.75458 1 GTATCAA 6130 0.0 39.965626 1 CAACGCA 6795 0.0 34.949863 5 ATCAACG 6735 0.0 34.854366 3 TCAACGC 6835 0.0 34.775803 4 AACGCAG 7180 0.0 33.2609 6 TATCAAC 7525 0.0 32.176193 2 ACGCAGA 8270 0.0 27.794594 7 CGCAGAG 8255 0.0 27.325754 8 GCAGAGT 9165 0.0 23.626406 9 GTACATG 6485 0.0 21.956984 1 TACATGG 6585 0.0 20.896614 2 GAGTACT 5995 0.0 20.522263 12-13 ACATGGG 6330 0.0 20.27581 3 CAGAGTA 9430 0.0 19.656523 10-11 GTGGTAT 1710 0.0 19.625834 1 AGAGTAC 8575 0.0 19.230032 10-11 GTACTTT 6735 0.0 18.604004 14-15 GTATAAG 1455 0.0 18.45233 1 TCTAACG 345 9.567884E-10 18.066502 2 >>END_MODULE