FastQCFastQC Report
Sat 18 Jun 2016
SRR1931113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790026
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71450.2560908034548782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.21541017897324255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38270.13716718052089838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.052.1019441
GTATCAA69450.041.695541
ATCAACG83900.034.1332863
TCAACGC85500.033.6026154
CAACGCA85900.033.225015
TATCAAC89350.032.26392
AACGCAG89750.031.8526696
ACGCAGA102850.027.610917
CGCAGAG105650.026.6993568
GCAGAGT118150.023.1913419
GTACATG72250.021.5206031
GTGGTAT20700.020.673631
GAGTACT75000.020.48315412-13
AGAGTAC107950.020.23573710-11
TACATGG73300.020.2149832
ACATGGG72000.019.986323
CAGAGTA115900.019.7900810-11
TGGTATC19450.019.7782632
ACGTGCG2001.2886147E-518.9951979
GTACTTT82100.018.6250114-15
Produced by FastQC (version 0.11.5)