##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931113_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2790026 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29190301452388 31.0 31.0 34.0 30.0 34.0 2 31.290744602380048 31.0 31.0 34.0 28.0 34.0 3 31.45328609840912 31.0 31.0 34.0 28.0 34.0 4 35.10557141761403 35.0 35.0 37.0 32.0 37.0 5 34.97252857141833 35.0 35.0 37.0 32.0 37.0 6 35.08531390030057 36.0 35.0 37.0 32.0 37.0 7 34.954226949856384 36.0 35.0 37.0 32.0 37.0 8 34.996981390137584 36.0 35.0 37.0 32.0 37.0 9 36.41312554076557 38.0 35.0 39.0 32.0 39.0 10-11 36.288994977107734 38.0 35.0 39.0 32.0 39.0 12-13 36.23200231825797 38.0 35.0 39.0 32.0 39.0 14-15 37.19212670419559 39.0 36.0 41.0 31.5 41.0 16-17 37.23819688418674 39.0 36.0 40.5 32.0 41.0 18-19 37.29770636546039 39.0 36.0 40.5 32.0 41.0 20-21 37.277269638347455 39.0 36.0 40.0 31.5 41.0 22-23 37.16689934072299 39.0 36.0 40.0 31.0 41.0 24-25 37.03543085261571 39.0 36.0 40.0 31.0 41.0 26-27 36.79857857955446 39.0 36.0 40.0 30.5 41.0 28-29 36.526615343369556 38.5 35.0 40.0 30.0 41.0 30-31 36.31825814526459 38.0 35.0 40.0 30.0 41.0 32-33 36.15924224362067 38.0 35.0 40.0 30.0 41.0 34-35 35.929069836625175 38.0 34.5 40.0 29.0 41.0 36-37 36.17667057582976 38.0 35.0 40.0 30.0 41.0 38-39 36.167146471036475 38.0 35.0 40.0 30.0 41.0 40-41 36.013472813515 38.0 35.0 40.0 29.5 41.0 42-43 35.86661934333229 38.0 35.0 40.0 29.0 41.0 44-45 35.65062924144793 38.0 34.5 40.0 28.0 41.0 46-47 35.45503303553444 38.0 34.0 40.0 27.5 41.0 48-49 35.21201200275553 38.0 34.0 40.0 27.0 41.0 50-51 34.869189211856806 38.0 33.5 40.0 26.0 41.0 52-53 34.76901917759906 38.0 33.0 40.0 26.0 41.0 54-55 34.548234138319856 38.0 33.0 40.0 25.0 41.0 56-57 34.34601666794503 38.0 33.0 40.0 24.5 41.0 58-59 34.11776216422356 37.5 33.0 40.0 24.0 41.0 60-61 33.848749975806676 37.0 32.5 40.0 23.0 41.0 62-63 33.60297771418618 37.0 32.0 40.0 22.5 41.0 64-65 33.37566549559036 36.0 32.0 39.0 22.5 41.0 66-67 33.15131525656034 36.0 32.0 39.0 22.0 41.0 68-69 32.839690382813636 36.0 31.0 39.0 22.0 40.0 70-71 32.01678998690335 35.0 31.0 38.5 19.0 40.0 72-73 31.98832609445217 35.0 31.0 38.0 20.0 40.0 74-75 31.679509079843697 35.0 30.5 37.5 20.0 39.5 76-77 30.95554396267275 34.0 30.0 36.0 18.5 39.0 78-79 30.967133998034427 34.0 30.0 36.0 18.0 39.0 80-81 30.680972686276043 34.0 30.0 36.0 17.5 38.5 82-83 30.319502936531777 34.0 30.0 36.0 14.5 37.0 84-85 29.945778283069764 34.0 29.0 35.0 11.0 37.0 86-87 29.601166619952647 34.0 29.0 35.0 7.5 36.5 88-89 29.274790091561872 34.0 29.0 35.0 4.5 36.0 90-91 28.959519552864382 34.0 29.0 35.0 2.0 36.0 92-93 28.61876824803783 34.0 29.0 35.0 2.0 35.5 94-95 28.232342996086775 34.0 29.0 35.0 2.0 35.0 96-97 27.862768124741493 33.0 28.0 35.0 2.0 35.0 98-99 27.400121898505603 33.0 27.0 35.0 2.0 35.0 100-101 26.72940592668312 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8166233684443043 1101 2 0.7176609753335761 1101 3 0.5203960253131115 1101 4 0.8500587534830686 1101 5 0.7938233346766808 1101 6 0.7990682756089384 1101 7 0.7803068126536985 1101 8 0.7421304124907238 1101 9 1.1515754828543479 1101 10-11 0.9453660622947595 1101 12-13 1.0888920279794263 1101 14-15 1.4662509348190582 1101 16-17 1.3170080495465726 1101 18-19 0.9425582403709782 1101 20-21 0.8314086823908582 1101 22-23 0.7938678622791073 1101 24-25 0.7545914502334625 1101 26-27 1.1110570784781615 1101 28-29 1.0163772323647535 1101 30-31 1.135484420662955 1101 32-33 1.3011111513154177 1101 34-35 0.9532075665457356 1101 36-37 1.291456991755794 1101 38-39 1.0687916466864138 1101 40-41 1.060433945747917 1101 42-43 1.065398200991858 1101 44-45 1.094125080695278 1101 46-47 1.076019436159278 1101 48-49 0.9428944297088222 1101 50-51 0.8436051367893285 1101 52-53 0.8470804182336522 1101 54-55 0.9125279829782684 1101 56-57 0.7502161455688139 1101 58-59 0.9678297185843334 1101 60-61 0.9811657251495376 1101 62-63 0.9099688409315405 1101 64-65 0.8978122624943197 1101 66-67 0.6276722492344007 1101 68-69 0.5733140010001208 1101 70-71 0.9565451354159897 1101 72-73 0.45675659234235866 1101 74-75 0.13660132852329454 1101 76-77 0.1354722121785592 1101 78-79 0.3487484172487534 1101 80-81 0.23207259920473078 1101 82-83 0.12208758102049799 1101 84-85 0.16665923031412078 1101 86-87 0.07774526426658568 1101 88-89 -0.041996602527618165 1101 90-91 0.025428786461990427 1101 92-93 -0.15725468426465028 1101 94-95 -0.12588910559833266 1101 96-97 -0.1289076039930741 1101 98-99 -0.20228283584707896 1101 100-101 -0.2426746768097061 1102 1 0.9151058373125203 1102 2 0.8494055888951912 1102 3 0.6338115806556743 1102 4 0.8941454147785564 1102 5 0.9224096937249087 1102 6 0.936050655432048 1102 7 0.9340646270343385 1102 8 0.9742360620629427 1102 9 1.4572546946010903 1102 10-11 1.1849174963638944 1102 12-13 1.203626059881934 1102 14-15 1.7587949647002574 1102 16-17 1.508134166524954 1102 18-19 1.3366444983537988 1102 20-21 1.2387969038895434 1102 22-23 0.9259581780618547 1102 24-25 0.643814390088636 1102 26-27 1.0670545197360681 1102 28-29 1.2135170180048647 1102 30-31 1.4859927448782528 1102 32-33 1.5726630336151786 1102 34-35 1.344053404944205 1102 36-37 1.6993740668753858 1102 38-39 1.5276025101266413 1102 40-41 1.5579990839070135 1102 42-43 1.7361763239841892 1102 44-45 1.7101185947949347 1102 46-47 1.683373439345928 1102 48-49 1.3812243246039628 1102 50-51 1.3603308689916105 1102 52-53 1.4692093460640976 1102 54-55 1.2259407546726138 1102 56-57 1.2701671138197241 1102 58-59 1.2770411980332668 1102 60-61 1.561426646303012 1102 62-63 1.6341368622542802 1102 64-65 1.547489106242132 1102 66-67 1.3706697132676098 1102 68-69 1.2315205122696256 1102 70-71 1.5230952051811748 1102 72-73 1.0477416368759833 1102 74-75 0.9367804626125782 1102 76-77 0.9874152618992618 1102 78-79 1.0268188682219055 1102 80-81 0.96611150037225 1102 82-83 0.9659554807800603 1102 84-85 1.0109604539489432 1102 86-87 0.9586482196469213 1102 88-89 0.874913949681762 1102 90-91 0.9983815713596869 1102 92-93 0.8286245879631764 1102 94-95 0.7602413355245048 1102 96-97 0.6609642026800273 1102 98-99 0.829370051760911 1102 100-101 0.7129861565323274 1103 1 0.6372040146462226 1103 2 0.48071171062343154 1103 3 0.3012137664173551 1103 4 0.7085115997039964 1103 5 0.6924009876341515 1103 6 0.6328418886729779 1103 7 0.581256526491444 1103 8 0.498161414823592 1103 9 0.9024933521666725 1103 10-11 0.7515278409403123 1103 12-13 0.8828077582980356 1103 14-15 1.213940229655833 1103 16-17 0.9946942374475896 1103 18-19 0.8539942851791338 1103 20-21 0.7417192068381695 1103 22-23 0.6272597638568982 1103 24-25 0.3794399988606898 1103 26-27 0.7321883053278526 1103 28-29 0.6165350769155467 1103 30-31 0.9769446460128819 1103 32-33 0.9533336595928787 1103 34-35 0.8783783344772118 1103 36-37 1.117521520493618 1103 38-39 0.8048529526048043 1103 40-41 0.8456691262203222 1103 42-43 1.0093052870134258 1103 44-45 1.0477893387037511 1103 46-47 1.0511982063295022 1103 48-49 0.8296774326405298 1103 50-51 0.6247468041332809 1103 52-53 0.2460463990098063 1103 54-55 0.4055312310102437 1103 56-57 0.5783246120783545 1103 58-59 0.773926547552378 1103 60-61 0.8038219422391535 1103 62-63 0.5675763782574421 1103 64-65 0.5251341449528582 1103 66-67 0.5124861298456977 1103 68-69 0.4106551091896051 1103 70-71 0.7375734246610541 1103 72-73 0.25479461096298195 1103 74-75 0.033195530298414155 1103 76-77 0.16842816400414407 1103 78-79 0.14987430561861714 1103 80-81 0.06606028390023866 1103 82-83 0.13762750584956507 1103 84-85 0.09318673789236698 1103 86-87 0.20708050842804582 1103 88-89 0.01342853167431457 1103 90-91 0.029089105990912856 1103 92-93 -0.22462883653237498 1103 94-95 -0.2083987642691767 1103 96-97 -0.22879488361243716 1103 98-99 -0.2654194033689805 1103 100-101 -0.3452692891889342 1104 1 0.4937313051270955 1104 2 0.394234783569118 1104 3 0.2216166522984473 1104 4 0.6935402187476711 1104 5 0.34675257870286913 1104 6 0.5559253539757023 1104 7 0.5104241434864036 1104 8 0.426427097854976 1104 9 0.7229244441504008 1104 10-11 0.5886460954101054 1104 12-13 0.6667643916459198 1104 14-15 1.0152959835085227 1104 16-17 0.8436376774447965 1104 18-19 0.6433587927665556 1104 20-21 0.6256045328824129 1104 22-23 0.33539596334448163 1104 24-25 0.3644404733199451 1104 26-27 0.2919798216273364 1104 28-29 0.6695728926251192 1104 30-31 0.8207144808318603 1104 32-33 0.8319674061184443 1104 34-35 0.6901641560353227 1104 36-37 0.9252341824345578 1104 38-39 0.482093203851349 1104 40-41 0.6551590879083165 1104 42-43 0.8481846158004558 1104 44-45 0.8680066933311963 1104 46-47 0.8033775276266937 1104 48-49 0.6856492905168352 1104 50-51 0.5076864798307739 1104 52-53 0.40797538114937737 1104 54-55 0.5764972392730598 1104 56-57 0.6156966984025587 1104 58-59 0.49763846758362007 1104 60-61 0.719856496406635 1104 62-63 0.03962119304839007 1104 64-65 -0.8791643097556587 1104 66-67 -0.3466202349982339 1104 68-69 0.14203119942699516 1104 70-71 0.6729230842115506 1104 72-73 0.25506694442125166 1104 74-75 0.11092871933051285 1104 76-77 -0.11801019112791522 1104 78-79 -0.09734526320327319 1104 80-81 -0.08975251862521105 1104 82-83 -0.2787370935538007 1104 84-85 -0.4256728250914428 1104 86-87 -0.07248414714144857 1104 88-89 -0.040491801311496545 1104 90-91 0.04521549147728976 1104 92-93 0.12358888074496832 1104 94-95 0.1612717779939139 1104 96-97 0.10588821067391763 1104 98-99 0.16231729989128496 1104 100-101 -0.09997228000472447 1105 1 -0.3219479663277873 1105 2 -1.750971595342687 1105 3 -0.38097879909555843 1105 4 0.3341450866663891 1105 5 0.494055012191545 1105 6 -0.10356184409749858 1105 7 -0.19924560977579375 1105 8 -0.26556847958848095 1105 9 0.5036879705791293 1105 10-11 0.8248849360563923 1105 12-13 0.9369558145408874 1105 14-15 1.2523569696972103 1105 16-17 0.7977417684978718 1105 18-19 0.5249507013143315 1105 20-21 0.7638286053151901 1105 22-23 0.24071258610407398 1105 24-25 -0.21866784118991234 1105 26-27 0.9383549585378432 1105 28-29 0.27375937709274467 1105 30-31 0.8055013052282121 1105 32-33 0.9682384651550606 1105 34-35 0.367984724312187 1105 36-37 0.6727794658646147 1105 38-39 0.6423865206416792 1105 40-41 0.8693078799187219 1105 42-43 1.1799532665771082 1105 44-45 1.1949857238424144 1105 46-47 1.1752055694404646 1105 48-49 0.9835480584046294 1105 50-51 0.697297119760016 1105 52-53 0.869662961136406 1105 54-55 -0.03292401582739757 1105 56-57 0.14009995215403137 1105 58-59 -0.4556741665910309 1105 60-61 0.1971975072403893 1105 62-63 0.8122765579398958 1105 64-65 1.0985896330551697 1105 66-67 0.9375833165085865 1105 68-69 0.7995013003371554 1105 70-71 0.1651772795449027 1105 72-73 -0.21864331976013673 1105 74-75 -0.24791382852520982 1105 76-77 -0.011977585834497262 1105 78-79 -0.5188696896382616 1105 80-81 -0.3841183952784313 1105 82-83 -0.3064354484625511 1105 84-85 -0.2414638607067694 1105 86-87 -0.603658157985091 1105 88-89 -0.8937802893440931 1105 90-91 -0.9353631969400453 1105 92-93 -0.6391366303366013 1105 94-95 -0.9543382321587615 1105 96-97 -1.0747753187201248 1105 98-99 -1.4563163202931975 1105 100-101 -0.7076730401139812 1106 1 0.3094627184657206 1106 2 0.3849353153843005 1106 3 -0.25690868809853384 1106 4 0.49848001390796526 1106 5 0.09612022667729292 1106 6 0.5133780302872282 1106 7 0.4768821943050554 1106 8 0.5499368249653998 1106 9 1.0486390133284758 1106 10-11 0.24264501014875606 1106 12-13 0.5203747922696778 1106 14-15 0.24622781333643218 1106 16-17 0.04087409957164567 1106 18-19 0.5874195346688964 1106 20-21 0.6438749549717997 1106 22-23 0.6221069701589172 1106 24-25 0.48019818298654116 1106 26-27 0.8544490814139607 1106 28-29 0.7048312319019061 1106 30-31 0.43398142120980054 1106 32-33 0.4451598950705318 1106 34-35 -0.9058712232822543 1106 36-37 -0.6424492502713903 1106 38-39 -0.5537577519024808 1106 40-41 0.6786105699599148 1106 42-43 0.8446446086834882 1106 44-45 0.8626320596617489 1106 46-47 0.4143195587763273 1106 48-49 0.15958707325297894 1106 50-51 0.740602049973468 1106 52-53 0.17796978611417558 1106 54-55 0.7774551781403858 1106 56-57 0.5676421204877329 1106 58-59 0.9481119027243139 1106 60-61 1.067053064875033 1106 62-63 0.9806553263064046 1106 64-65 0.9718064187277093 1106 66-67 0.712186267728498 1106 68-69 0.6769640615355286 1106 70-71 1.0619286628800957 1106 72-73 0.45501230171469587 1106 74-75 0.544790332985233 1106 76-77 0.5409291346298488 1106 78-79 0.6567068769229998 1106 80-81 0.5281426429685538 1106 82-83 0.5094079784873387 1106 84-85 0.5338632678630795 1106 86-87 0.588957843260264 1106 88-89 0.6822828097146072 1106 90-91 0.8023532530211419 1106 92-93 0.34801078756873594 1106 94-95 0.5303582045836244 1106 96-97 0.8541860359498799 1106 98-99 0.87479285324887 1106 100-101 0.7748840688163625 1107 1 0.503145823397201 1107 2 0.4329655577903573 1107 3 0.21859604665522525 1107 4 -0.8899348438827062 1107 5 0.2870975198652488 1107 6 0.4120797241287164 1107 7 0.6243622716615889 1107 8 0.485605442217242 1107 9 0.9331522839763338 1107 10-11 0.8661128838441954 1107 12-13 0.9458722331686147 1107 14-15 1.2329579990588257 1107 16-17 1.0694949775016909 1107 18-19 0.9200920447552576 1107 20-21 0.24917008517752492 1107 22-23 0.5837114931824914 1107 24-25 0.5016279452485577 1107 26-27 0.9365130756122255 1107 28-29 0.9829819687896446 1107 30-31 1.1236170619020172 1107 32-33 1.2565007973412108 1107 34-35 1.049461834968561 1107 36-37 1.2062120147747848 1107 38-39 1.129289870717642 1107 40-41 0.5932205779623132 1107 42-43 0.43086151542217976 1107 44-45 0.2625393235075677 1107 46-47 0.7554143872248602 1107 48-49 0.9892515976102843 1107 50-51 -0.7834370367525523 1107 52-53 0.7548784837234592 1107 54-55 1.2061421175555438 1107 56-57 1.2048912118818151 1107 58-59 1.2338934705279954 1107 60-61 1.5304366632878867 1107 62-63 1.4583098726703128 1107 64-65 1.290010004803527 1107 66-67 1.141432958402369 1107 68-69 1.1736455659868597 1107 70-71 1.5147868119914953 1107 72-73 1.1478937078031919 1107 74-75 1.1595726843573182 1107 76-77 1.114956342792297 1107 78-79 1.0493423759060292 1107 80-81 1.199669934489343 1107 82-83 1.3305184783039508 1107 84-85 1.2190750814071443 1107 86-87 1.192336982001887 1107 88-89 1.2585148459921562 1107 90-91 1.2757700697328218 1107 92-93 1.230132507418027 1107 94-95 1.1654701965145406 1107 96-97 1.1972991782258937 1107 98-99 1.3459625374874733 1107 100-101 1.309050985558052 1108 1 0.49361754929901736 1108 2 0.5102624016954955 1108 3 0.2930630560164609 1108 4 0.7075603246267548 1108 5 0.5670035482722611 1108 6 0.5306436937819257 1108 7 0.5742651030228174 1108 8 0.5572793471392572 1108 9 0.8671642711019345 1108 10-11 0.7232503783661173 1108 12-13 0.9453345277853558 1108 14-15 1.1681134688808612 1108 16-17 0.9841779041995409 1108 18-19 0.8617751905764663 1108 20-21 0.7164692909451418 1108 22-23 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0.6189322546230684 2311 68-69 1.2883422371107969 2311 70-71 1.6518655707594156 2311 72-73 1.1860061424103634 2311 74-75 1.2384091612381631 2311 76-77 1.2434359449224033 2311 78-79 1.192874497566013 2311 80-81 1.0698302788720113 2311 82-83 1.285729377499294 2311 84-85 1.2225485254684187 2311 86-87 1.1599239867172066 2311 88-89 1.333363533443773 2311 90-91 1.6189324140216534 2311 92-93 1.4247776267086145 2311 94-95 1.5410462691914795 2311 96-97 1.9186710493404462 2311 98-99 2.301842613495637 2311 100-101 2.195369516066883 2312 1 0.005132177529475257 2312 2 -0.1104673683678925 2312 3 -0.32285465214526 2312 4 0.2664148865013587 2312 5 -0.9445300800026928 2312 6 -0.06441004147049512 2312 7 0.32744634781433746 2312 8 -2.679612726595348 2312 9 -5.095882325905915 2312 10-11 -0.4756143088486624 2312 12-13 -5.711340680884433 2312 14-15 -2.0186074240731244 2312 16-17 -2.5639834807353523 2312 18-19 -2.3253609375271296 2312 20-21 -3.645306227136764 2312 22-23 -2.07293880394527 2312 24-25 -2.148916788987165 2312 26-27 -3.0996078939046754 2312 28-29 -6.355063453241829 2312 30-31 -6.8802084580715785 2312 32-33 -1.9063114619302794 2312 34-35 0.3782509017672808 2312 36-37 0.9643390093824706 2312 38-39 0.9230467486751621 2312 40-41 -2.451075531864909 2312 42-43 -4.853663767949669 2312 44-45 -3.048472974280486 2312 46-47 0.45697430799079086 2312 48-49 -1.4936491121949302 2312 50-51 -7.428654792887691 2312 52-53 -1.5551378858154479 2312 54-55 0.7796977464759749 2312 56-57 1.1143841688533058 2312 58-59 1.2775079747648164 2312 60-61 1.4718268360621778 2312 62-63 1.47353629503192 2312 64-65 1.4823844132991866 2312 66-67 1.1505242426545905 2312 68-69 1.4890384349205021 2312 70-71 1.6857542654965023 2312 72-73 1.3304285102682485 2312 74-75 0.995867666199505 2312 76-77 1.1817880804394854 2312 78-79 1.047440620651269 2312 80-81 1.356170979938888 2312 82-83 0.6331128438811504 2312 84-85 0.811043002613264 2312 86-87 1.246156311813703 2312 88-89 1.1986023336118308 2312 90-91 1.4849376993135408 2312 92-93 1.7143723555527188 2312 94-95 1.821297889922704 2312 96-97 1.9733852077185787 2312 98-99 1.9288941981015384 2312 100-101 2.123874359608891 2313 1 -0.8160127175923293 2313 2 -0.3070218022674247 2313 3 -0.4378277525502625 2313 4 -1.3095628684403096 2313 5 -2.698601288368767 2313 6 -2.343621792664351 2313 7 -0.9794387395337836 2313 8 -4.570321787829705 2313 9 -2.766220233177876 2313 10-11 -0.23187375393116838 2313 12-13 -2.7677089333174862 2313 14-15 -2.6248336224651965 2313 16-17 -6.567876365943597 2313 18-19 -3.640243365140016 2313 20-21 -4.314785765310013 2313 22-23 -2.3437058957665897 2313 24-25 -0.5651109496593492 2313 26-27 -4.372829195965966 2313 28-29 -4.067751599285472 2313 30-31 -3.1572198828433855 2313 32-33 -6.913172369867169 2313 34-35 -2.601172419274853 2313 36-37 -4.455687587969884 2313 38-39 -1.3753936793004158 2313 40-41 -0.07856752243494469 2313 42-43 -2.06083900823257 2313 44-45 -4.305222052440431 2313 46-47 -1.4594556019409026 2313 48-49 0.13487050335243822 2313 50-51 1.937687466678291 2313 52-53 1.6804179384002325 2313 54-55 -3.0365447730186688 2313 56-57 -5.850277621632088 2313 58-59 -1.2303986927078299 2313 60-61 -6.804670776220959 2313 62-63 -0.07641836857202122 2313 64-65 -0.5619243577822388 2313 66-67 -2.1567658498316575 2313 68-69 -3.1290900786501936 2313 70-71 -3.133129854675172 2313 72-73 0.7586579728808331 2313 74-75 2.556715154563168 2313 76-77 -0.5159934585800379 2313 78-79 1.4950921953354275 2313 80-81 2.866616485590825 2313 82-83 2.6943411579716994 2313 84-85 3.047239581226574 2313 86-87 3.2378204597917026 2313 88-89 -1.343137375026263 2313 90-91 2.6574940675659775 2313 92-93 3.2878112723150963 2313 94-95 3.4796246746654944 2313 96-97 3.275416278262007 2313 98-99 4.386265896100692 2313 100-101 5.046754127486544 2314 1 -28.759674689423314 2314 2 -14.751708101883303 2314 3 -5.007482041922863 2314 4 -4.201240205188068 2314 5 -1.2800353472676136 2314 6 -0.949895800346809 2314 7 -0.7533183810190636 2314 8 -3.9253794062676057 2314 9 -3.572238158914111 2314 10-11 -0.7159966732653587 2314 12-13 0.018526662073035993 2314 14-15 -2.2750256839248664 2314 16-17 -2.3061606169039095 2314 18-19 -4.076863083449872 2314 20-21 -2.3844400546826137 2314 22-23 -4.736087457866461 2314 24-25 -1.3305398869192686 2314 26-27 -4.616619208770064 2314 28-29 -3.6558437887861146 2314 30-31 -5.146400420615471 2314 32-33 -3.8355155196751056 2314 34-35 -4.043618001861276 2314 36-37 -7.378286819724046 2314 38-39 -7.667199057021293 2314 40-41 -6.466467650475913 2314 42-43 -4.3804292771186155 2314 44-45 -6.665913473695234 2314 46-47 -8.76713806032759 2314 48-49 -8.289201199592512 2314 50-51 -4.423836220901734 2314 52-53 -5.782898322803348 2314 54-55 -7.182319420905998 2314 56-57 -4.863785944295344 2314 58-59 -4.205558743924222 2314 60-61 -2.397564502213278 2314 62-63 -1.3099010830406463 2314 64-65 -3.0093635383200095 2314 66-67 -2.533654454185047 2314 68-69 -1.1628928955516002 2314 70-71 0.08959741805210086 2314 72-73 -0.5883970847376006 2314 74-75 -0.899430812146182 2314 76-77 -1.5568385290025724 2314 78-79 -3.663230467916815 2314 80-81 -2.9332554734360627 2314 82-83 -3.514877792092321 2314 84-85 -2.311723286891226 2314 86-87 -0.7918850459701439 2314 88-89 -0.3500515875742778 2314 90-91 0.18175783197058593 2314 92-93 0.2636115283970426 2314 94-95 0.036026723321064935 2314 96-97 0.28629976097647614 2314 98-99 -0.23620529468035656 2314 100-101 0.023386649893716083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 21.0 4 52.0 5 111.0 6 218.0 7 620.0 8 1660.0 9 2958.0 10 4550.0 11 6688.0 12 8856.0 13 11361.0 14 13516.0 15 16449.0 16 18884.0 17 20801.0 18 19132.0 19 16184.0 20 16127.0 21 16767.0 22 18721.0 23 21418.0 24 25350.0 25 31608.0 26 40269.0 27 50984.0 28 64631.0 29 81069.0 30 99636.0 31 121839.0 32 147502.0 33 178197.0 34 217682.0 35 268510.0 36 338431.0 37 424646.0 38 392678.0 39 91814.0 40 77.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.86775857057243 21.42648489002609 15.385366238781009 23.320390300620474 2 14.659612491066392 21.857968348682057 40.70686079627932 22.775558363972234 3 18.083024034782422 26.409442581584024 31.24575479898133 24.261778584652223 4 12.438992307215333 18.281107247733615 39.28381081548966 29.996089629561396 5 11.968834643180106 38.64558725543643 36.55538793435605 12.830190167027416 6 29.440908435978734 39.57142334874298 18.06556641407643 12.922101801201851 7 25.56348937250047 33.44868470759771 23.20197016085155 17.78585575905027 8 24.186333747427444 37.28445541367715 20.71640192600356 17.81280891289185 9 24.760450261036993 16.582246903792296 21.46560641370367 37.19169642146704 10-11 23.392577703576954 27.481303041620404 29.56364922764161 19.562470027161037 12-13 24.221315500285662 24.932204932857257 29.497234075954847 21.349245490902234 14-15 21.40790085827157 26.749266852710335 27.171108799702942 24.671723489315152 16-17 20.867368261084305 29.78663998113279 27.871209802345927 21.474781955436974 18-19 20.725559546756912 28.72831651031209 29.94285716333826 20.603266779592737 20-21 21.483204816012467 27.875690047332892 30.01280274807475 20.628302388579893 22-23 21.241414954555978 27.808289241749 29.739669092689457 21.210626711005563 24-25 21.14644902536042 27.96026877511163 29.864683977022764 21.028598222505185 26-27 21.1401784906171 28.043476507577203 29.744218411291918 21.072126590513776 28-29 21.055340612483807 27.99743430376725 29.868317200057636 21.078907883691304 30-31 21.350440998180094 27.888396949437766 29.75197828656324 21.0091837658189 32-33 21.27403264579623 27.944911966170693 29.625463305289195 21.15559208274388 34-35 21.423628988856713 27.9907296676978 29.640995055332926 20.944646288112565 36-37 21.302152021618603 27.854614571410192 29.648787304823344 21.194446102147865 38-39 21.229058789331805 28.016213544524703 29.66740656763965 21.087321098503843 40-41 21.45459954426156 27.85576227765278 29.638052636625208 21.05158554146046 42-43 21.3233486188879 27.868532522245538 29.48064837322464 21.327470485641925 44-45 21.4320007526848 28.049265012253528 29.29689383112083 21.221840403940842 46-47 21.452775527552173 27.99548548674695 29.07461979154436 21.477119194156515 48-49 21.345744627460427 28.11164213946592 29.124652812132112 21.41796042094154 50-51 21.447516232097787 28.24825356636215 28.88300890063691 21.421221300903156 52-53 21.56454071862866 28.225163167884382 28.77414274472584 21.436153368761122 54-55 21.518575461760157 28.134723460219817 28.847559091029694 21.499141986990328 56-57 21.53338048975172 28.24295507661186 28.729997127245806 21.493667306390616 58-59 21.46435868536894 28.252030152693575 28.828374342002572 21.455236819934914 60-61 21.517053858317052 28.25743057961272 28.780040291181834 21.44547527088839 62-63 21.45536280573424 28.235948147337037 28.729990410200063 21.578698636728667 64-65 21.364472212663454 28.166831555402617 28.727524948382655 21.74117128355127 66-67 21.37932690722285 28.217392364083178 28.652789307594485 21.750491421099493 68-69 21.480514122711234 28.19800237028785 28.639030751913158 21.68245275508776 70-71 21.514810617757398 27.99971038113377 28.601368484986867 21.884110516121968 72-73 21.495126613074493 28.05276896373433 28.632413914848126 21.819690508343047 74-75 21.428767281632478 28.186814122260184 28.43554238945087 21.94887620665647 76-77 21.33860042881321 28.25321341091445 28.358983034566705 22.04920312570564 78-79 21.41767163059979 28.275530098201614 28.26938320097791 22.037415070220685 80-81 21.366911593270228 28.260823983488287 28.27806400636268 22.094200416878806 82-83 21.474166380676824 28.18623897520356 28.385198300727506 21.95439634339211 84-85 21.463696561858765 28.232435193233034 28.219765055868674 22.084103189039524 86-87 21.31354752132543 28.272829293697193 28.2616643351079 22.15195884986948 88-89 21.395992351021448 28.128616821659442 28.25102198454075 22.224368842778365 90-91 21.383444465930022 28.106633529098985 28.328408875243262 22.181513129727733 92-93 21.425558042274865 28.108409093841715 28.23762371269264 22.22840915119078 94-95 21.26372378786312 28.091177761437674 28.196052294614283 22.44904615608492 96-97 21.223033508619224 28.05192519677772 28.304612146141945 22.42042914846111 98-99 21.13853789982914 28.093551442970956 28.197692235731314 22.570218421468592 100-101 21.16475348197702 28.040246772508926 28.148815493390895 22.646184252123167 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 136.5 2 117.0 3 140.0 4 126.0 5 130.5 6 246.5 7 550.5 8 932.0 9 1220.0 10 1312.5 11 1317.5 12 1418.0 13 1590.5 14 1855.5 15 2142.0 16 2417.0 17 2735.0 18 3078.5 19 3430.5 20 3955.5 21 4781.5 22 6067.0 23 7851.0 24 10293.0 25 13812.5 26 18752.0 27 24665.0 28 31048.0 29 38347.0 30 45739.0 31 52968.0 32 61257.0 33 69285.5 34 76757.5 35 83631.5 36 91127.5 37 97779.5 38 100861.0 39 102299.5 40 103153.0 41 104400.5 42 104933.5 43 104740.0 44 104913.5 45 104723.0 46 102958.5 47 100345.0 48 98263.5 49 95287.5 50 91190.5 51 86429.0 52 81236.5 53 76059.5 54 70814.5 55 64682.0 56 57619.0 57 50447.0 58 44275.0 59 38621.5 60 31861.5 61 25194.0 62 19780.5 63 15213.0 64 11372.0 65 8455.5 66 6263.5 67 4689.5 68 3650.5 69 2910.5 70 2235.5 71 1690.0 72 1303.5 73 1014.5 74 786.0 75 591.5 76 442.5 77 311.5 78 223.0 79 174.0 80 119.5 81 85.0 82 61.5 83 57.5 84 55.5 85 38.5 86 27.5 87 19.5 88 12.5 89 13.0 90 10.5 91 4.0 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1238698133995884 2 0.0 3 0.003978457548424279 4 3.225776390614281E-4 5 1.7920979947857118E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01727582466973426 26-27 0.008727517234606416 28-29 0.005017874385399993 30-31 0.0017383350549421402 32-33 6.809972380185705E-4 34-35 0.0018100189747335689 36-37 7.706021377578559E-4 38-39 4.65945478644285E-4 40-41 0.0017383350549421402 42-43 0.0010035748770799986 44-45 4.838664585921422E-4 46-47 0.028655646936623532 48-49 0.01684572115098569 50-51 0.08661209608799345 52-53 0.0011290217367149985 54-55 0.03670216693321138 56-57 7.347601778621418E-4 58-59 9.498119372364273E-4 60-61 0.0030644875710835666 62-63 0.002956961691396424 64-65 0.009533961332259986 66-67 0.0056630296635228495 68-69 0.0024910162127521392 70-71 0.0054658988840964205 72-73 0.004605691846599279 74-75 0.0012007056565064267 76-77 0.0 78-79 1.7920979947857118E-5 80-81 2.329727393221425E-4 82-83 3.0465665911357095E-4 84-85 3.5841959895714236E-5 86-87 0.001684572115098569 88-89 0.003978457548424279 90-91 0.0014515993757764265 92-93 0.003369144230197138 94-95 9.13969977340713E-4 96-97 4.4802449869642795E-4 98-99 0.0018996238744728546 100-101 0.0031540924708228528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2790026.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.94451771710361 #Duplication Level Percentage of deduplicated Percentage of total 1 84.01236676795043 59.60216842624665 2 10.397635536150787 14.753104770208711 3 2.641101570310858 5.621150314427665 4 1.0625573735746578 3.0153048166002554 5 0.5075967934724578 1.8005604853825876 6 0.31705414192102965 1.349595191327854 7 0.20996056059446752 1.0426885497691043 8 0.15094978814701224 0.8567247935670997 9 0.10592099520049264 0.6763062528553136 >10 0.5459828195640061 7.142088813607914 >50 0.0333663979964686 1.614525124681942 >100 0.015101582502205318 1.7532126782581567 >500 1.5212723067551686E-4 0.0716987929087422 >1k 1.521272306677804E-4 0.22761578660967213 >5k 1.0141815377852027E-4 0.47325520354832257 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7145 0.2560908034548782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6010 0.21541017897324255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3827 0.13716718052089838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.168391979142847E-5 0.0 0.0 0.0 0.0 7 7.168391979142847E-5 0.0 0.0 0.0 0.0 8 7.168391979142847E-5 0.0 0.0 0.0 0.0 9 7.168391979142847E-5 3.5841959895714236E-5 0.0 0.0 0.0 10-11 7.168391979142847E-5 3.5841959895714236E-5 0.0 0.0 0.0 12-13 7.168391979142847E-5 3.5841959895714236E-5 0.0 0.0 1.7920979947857118E-5 14-15 8.960489973928559E-5 7.168391979142847E-5 0.0 0.0 3.5841959895714236E-5 16-17 1.4336783958285694E-4 7.168391979142847E-5 0.0 0.0 3.5841959895714236E-5 18-19 1.4336783958285694E-4 8.960489973928559E-5 0.0 5.3762939843571354E-5 8.960489973928559E-5 20-21 1.4336783958285694E-4 1.075258796871427E-4 0.0 7.168391979142847E-5 1.4336783958285694E-4 22-23 1.4336783958285694E-4 1.075258796871427E-4 0.0 8.960489973928559E-5 1.4336783958285694E-4 24-25 1.4336783958285694E-4 1.4336783958285694E-4 0.0 2.867356791657139E-4 1.7920979947857118E-4 26-27 1.4336783958285694E-4 1.7920979947857118E-4 0.0 7.52681157809999E-4 1.971307794264283E-4 28-29 1.4336783958285694E-4 1.7920979947857118E-4 0.0 0.002419332292960711 2.150517593742854E-4 30-31 1.4336783958285694E-4 1.7920979947857118E-4 0.0 0.007526811578099989 2.6881469921785677E-4 32-33 1.4336783958285694E-4 1.7920979947857118E-4 0.0 0.02200696337596854 2.867356791657139E-4 34-35 1.4336783958285694E-4 2.5089371926999965E-4 0.0 0.04340461343370994 2.867356791657139E-4 36-37 1.4336783958285694E-4 3.225776390614281E-4 0.0 0.0789419166703106 2.867356791657139E-4 38-39 1.4336783958285694E-4 3.5841959895714236E-4 0.0 0.1276332191886384 3.04656659113571E-4 40-41 1.4336783958285694E-4 3.942615588528566E-4 0.0 0.18024921631554688 3.225776390614281E-4 42-43 1.4336783958285694E-4 3.942615588528566E-4 0.0 0.23553543945468608 3.5841959895714236E-4 44-45 1.4336783958285694E-4 4.6594547864428507E-4 0.0 0.3049433947927367 3.5841959895714236E-4 46-47 1.6128881953071406E-4 5.376293984357135E-4 0.0 0.3842616520419523 3.5841959895714236E-4 48-49 1.7920979947857118E-4 5.376293984357135E-4 0.0 0.4691533340549514 3.5841959895714236E-4 50-51 1.7920979947857118E-4 5.376293984357135E-4 0.0 0.5533819398098799 3.763405789049995E-4 52-53 1.7920979947857118E-4 5.376293984357135E-4 0.0 0.6340801125150806 4.3010351874857083E-4 54-55 1.7920979947857118E-4 5.555503783835707E-4 0.0 0.7285595188001832 4.65945478644285E-4 56-57 1.7920979947857118E-4 5.734713583314278E-4 0.0 0.8382359160810688 4.8386645859214213E-4 58-59 1.7920979947857118E-4 5.734713583314278E-4 0.0 0.9606541301048808 5.197084184878564E-4 60-61 2.1505175937428542E-4 6.09313318227142E-4 0.0 1.0820866902315607 5.376293984357135E-4 62-63 3.225776390614281E-4 6.09313318227142E-4 0.0 1.2041285636764676 5.734713583314278E-4 64-65 3.225776390614281E-4 7.52681157809999E-4 0.0 1.3364212376515487 5.734713583314278E-4 66-67 3.225776390614281E-4 7.885231177057132E-4 0.0 1.4876384664515672 5.734713583314278E-4 68-69 3.225776390614281E-4 8.422860575492844E-4 0.0 1.6578160920364182 6.09313318227142E-4 70-71 3.4049861900928524E-4 8.781280174449987E-4 0.0 1.8388717524496188 6.451552781228562E-4 72-73 4.301035187485708E-4 8.960489973928558E-4 0.0 2.029353848315392 6.451552781228562E-4 74-75 4.301035187485708E-4 9.139699773407129E-4 0.0 2.225248080125418 6.451552781228562E-4 76-77 4.301035187485708E-4 9.498119372364272E-4 0.0 2.4377012974072647 6.451552781228562E-4 78-79 4.301035187485708E-4 9.677329171842843E-4 0.0 2.6744732844783528 6.630762580707133E-4 80-81 4.301035187485708E-4 9.677329171842843E-4 0.0 2.9263526576454844 6.989182179664277E-4 82-83 4.301035187485708E-4 9.677329171842843E-4 0.0 3.1890921446610174 7.168391979142847E-4 84-85 4.301035187485708E-4 0.0010035748770799986 0.0 3.451025187578897 7.347601778621418E-4 86-87 4.480244986964279E-4 0.0010035748770799986 0.0 3.7300548453670324 8.064440976535703E-4 88-89 4.65945478644285E-4 0.0011469427166628555 0.0 4.035733000337631 8.602070374971416E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3760 0.0 52.101944 1 GTATCAA 6945 0.0 41.69554 1 ATCAACG 8390 0.0 34.133286 3 TCAACGC 8550 0.0 33.602615 4 CAACGCA 8590 0.0 33.22501 5 TATCAAC 8935 0.0 32.2639 2 AACGCAG 8975 0.0 31.852669 6 ACGCAGA 10285 0.0 27.61091 7 CGCAGAG 10565 0.0 26.699356 8 GCAGAGT 11815 0.0 23.191341 9 GTACATG 7225 0.0 21.520603 1 GTGGTAT 2070 0.0 20.67363 1 GAGTACT 7500 0.0 20.483154 12-13 AGAGTAC 10795 0.0 20.235737 10-11 TACATGG 7330 0.0 20.214983 2 ACATGGG 7200 0.0 19.98632 3 CAGAGTA 11590 0.0 19.79008 10-11 TGGTATC 1945 0.0 19.778263 2 ACGTGCG 200 1.2886147E-5 18.995197 9 GTACTTT 8210 0.0 18.62501 14-15 >>END_MODULE