FastQCFastQC Report
Sat 18 Jun 2016
SRR1931112_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931112_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474553
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.19062028576474216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.14665274900153683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.049.7233961
GTATCAA53850.041.5850521
ATCAACG60550.035.960233
TCAACGC62800.035.4476134
CAACGCA62950.034.923485
AACGCAG66950.033.313876
TATCAAC66950.032.804192
ACGCAGA73450.029.6592487
CGCAGAG72750.029.2238628
GCAGAGT82950.024.480499
GTGGTAT16250.022.7705961
GTACATG52450.021.5307621
GAGTACT55550.021.40070212-13
TGGTATC15600.021.2563272
AGAGTAC76100.020.32623510-11
TAGGCCG1656.1367566E-520.2314245
TACATGG52750.020.1341612
CAGAGTA84800.019.95535310-11
GTACTTT64050.018.7592314-15
ACATGGG51500.018.67733
Produced by FastQC (version 0.11.5)