##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931112_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474553 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.647854380164823 31.0 28.0 31.0 23.0 34.0 2 28.337009148723023 31.0 28.0 33.0 16.0 34.0 3 28.398158374461975 31.0 28.0 34.0 16.0 34.0 4 31.57555687835338 35.0 32.0 37.0 18.0 37.0 5 31.684070213893175 35.0 32.0 37.0 19.0 37.0 6 31.947406258827353 35.0 32.0 37.0 19.0 37.0 7 31.595795685119697 35.0 32.0 37.0 17.0 37.0 8 31.80304402451675 35.0 32.0 37.0 18.0 37.0 9 32.80506135855647 37.0 32.0 39.0 17.0 39.0 10-11 32.81538362686109 37.0 32.0 39.0 17.5 39.0 12-13 32.84017436684525 37.0 32.0 39.0 17.5 39.0 14-15 33.614362674794194 38.0 32.0 40.0 17.0 41.0 16-17 33.62302625969215 38.0 32.0 40.0 17.5 41.0 18-19 33.6296379588556 38.0 32.0 40.0 17.5 41.0 20-21 33.8946338995366 38.0 32.0 40.0 20.0 41.0 22-23 33.783959567647166 38.0 32.0 40.0 19.0 41.0 24-25 33.702527688839155 38.0 32.0 40.0 19.5 41.0 26-27 33.331568368105266 38.0 31.5 40.0 17.5 41.0 28-29 33.307821048892464 37.5 31.0 40.0 17.5 41.0 30-31 33.054561369265485 37.0 31.0 40.0 16.5 41.0 32-33 32.85434864397732 37.0 30.5 40.0 16.0 41.0 34-35 32.83183912407614 37.0 30.0 40.0 16.0 41.0 36-37 32.478191818886074 37.0 30.0 40.0 15.0 41.0 38-39 32.32567174758431 37.0 30.0 40.0 14.0 41.0 40-41 32.17254368768824 36.5 30.0 40.0 9.0 41.0 42-43 32.39811068908203 37.0 30.0 40.0 10.5 41.0 44-45 32.46283247923968 37.0 30.0 40.0 11.0 41.0 46-47 32.271903450845464 37.0 30.0 40.0 9.0 41.0 48-49 32.06634895271995 37.0 30.0 40.0 8.5 41.0 50-51 31.544901846919423 36.5 30.0 39.5 8.0 40.5 52-53 31.518065484958292 36.0 30.0 39.5 7.0 40.5 54-55 31.52827722825092 36.0 30.0 40.0 2.0 41.0 56-57 31.297855410653966 36.0 29.0 40.0 2.0 41.0 58-59 31.166127983518642 36.0 29.0 40.0 2.0 41.0 60-61 31.040932645209054 35.5 29.0 40.0 2.0 41.0 62-63 30.907825372905734 35.0 29.0 39.0 2.0 41.0 64-65 30.575400890585087 35.0 29.0 39.0 2.0 41.0 66-67 30.370258790173416 35.0 28.5 39.0 2.0 40.0 68-69 30.2018000826816 35.0 29.0 39.0 2.0 40.0 70-71 29.92765804571573 34.5 28.5 38.0 2.0 40.0 72-73 29.599803277602057 34.0 28.0 37.5 2.0 40.0 74-75 29.17969508028319 34.0 28.0 37.0 2.0 39.5 76-77 28.876454656659202 34.0 28.0 36.5 2.0 39.0 78-79 28.46135483863146 34.0 27.5 36.0 2.0 39.0 80-81 28.055606002376997 33.0 26.5 36.0 2.0 38.5 82-83 27.715740580217922 33.0 26.0 35.0 2.0 37.0 84-85 27.374278910170847 33.0 26.0 35.0 2.0 37.0 86-87 27.045392844687505 33.0 26.0 35.0 2.0 36.0 88-89 26.69280168984055 33.0 26.0 35.0 2.0 36.0 90-91 26.30875616727546 32.0 25.0 35.0 2.0 36.0 92-93 25.951719563088766 32.0 24.0 35.0 2.0 35.0 94-95 25.6536970515483 32.0 23.5 35.0 2.0 35.0 96-97 25.26125081984504 32.0 20.0 35.0 2.0 35.0 98-99 24.916143238799087 32.0 18.5 35.0 2.0 35.0 100-101 24.336672320212983 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.176082776143595 1101 2 5.79482034713153 1101 3 5.380495825550863 1101 4 6.6495520149431755 1101 5 6.068615985748988 1101 6 5.58880591806923 1101 7 6.208451157496494 1101 8 5.64488268738603 1101 9 6.40410058829541 1101 10-11 6.347420380076251 1101 12-13 6.224163958937833 1101 14-15 6.46879148403859 1101 16-17 6.446912776872804 1101 18-19 6.42142903742797 1101 20-21 5.82331792361056 1101 22-23 5.789159168430757 1101 24-25 5.667422694282269 1101 26-27 5.797473773348305 1101 28-29 5.752709799165224 1101 30-31 5.834565136752932 1101 32-33 5.680094882429092 1101 34-35 5.616277220058247 1101 36-37 5.7867797669598104 1101 38-39 5.748072642528243 1101 40-41 5.8824940180006955 1101 42-43 5.615858049381821 1101 44-45 5.604016488009339 1101 46-47 5.487566115472209 1101 48-49 5.319485698994232 1101 50-51 5.252921284368476 1101 52-53 5.060831842991313 1101 54-55 5.290333195168408 1101 56-57 5.383801120090411 1101 58-59 5.171687761784874 1101 60-61 5.12548598343766 1101 62-63 5.150139209613112 1101 64-65 5.034676329074433 1101 66-67 4.960017910681227 1101 68-69 5.04954705551836 1101 70-71 4.934724344441701 1101 72-73 4.746513704675273 1101 74-75 4.9309446347715244 1101 76-77 4.815894630944211 1101 78-79 4.676053607492403 1101 80-81 4.519603619935296 1101 82-83 4.254264450194416 1101 84-85 4.231971149927208 1101 86-87 4.106459902601067 1101 88-89 4.1999064444241085 1101 90-91 4.210697118566834 1101 92-93 4.1353590404131175 1101 94-95 4.125380407113937 1101 96-97 4.033990903267497 1101 98-99 3.8217058077872252 1101 100-101 3.930667682703774 1102 1 5.396560459950141 1102 2 5.93821521582845 1102 3 5.659669451221593 1102 4 6.926836937298454 1102 5 6.436628849542036 1102 6 5.882511720990234 1102 7 6.3788400029533 1102 8 6.0119765184510285 1102 9 6.757312983021972 1102 10-11 6.7719852942584 1102 12-13 6.720497600244112 1102 14-15 7.021333149224617 1102 16-17 6.980004173767018 1102 18-19 6.94562171892472 1102 20-21 6.309648366736248 1102 22-23 6.4140818790664795 1102 24-25 6.345570681205263 1102 26-27 6.445236379510131 1102 28-29 6.298012915614542 1102 30-31 6.410095723796754 1102 32-33 6.261696016006443 1102 34-35 6.288638276879265 1102 36-37 6.272293785651399 1102 38-39 6.197490529241577 1102 40-41 6.394564484579707 1102 42-43 6.3359275209713175 1102 44-45 6.157571798765883 1102 46-47 6.20502507215463 1102 48-49 5.960449506250544 1102 50-51 5.938587216638112 1102 52-53 5.714484653836692 1102 54-55 5.973566067134584 1102 56-57 6.013339427186821 1102 58-59 6.034182209388014 1102 60-61 5.813958541271429 1102 62-63 5.673896139004395 1102 64-65 5.769555153565388 1102 66-67 5.752172151824052 1102 68-69 5.618348137569463 1102 70-71 5.48069930091091 1102 72-73 5.3049795552136345 1102 74-75 5.362058934192298 1102 76-77 5.3511241448207585 1102 78-79 5.326191084838623 1102 80-81 5.400615311062122 1102 82-83 5.221523520589926 1102 84-85 5.0970512376995 1102 86-87 5.066125737148525 1102 88-89 5.250993159537291 1102 90-91 5.262968493073782 1102 92-93 5.3076754559633805 1102 94-95 5.263177349297941 1102 96-97 5.235807425423783 1102 98-99 5.048958970432476 1102 100-101 4.888993834937171 1103 1 5.181419229152201 1103 2 5.7307932756421565 1103 3 5.419475263921104 1103 4 6.836525128693708 1103 5 6.423307406936029 1103 6 5.682077078420836 1103 7 6.388527723325023 1103 8 5.823290660640922 1103 9 6.223890274843026 1103 10-11 6.491346054768506 1103 12-13 6.52326345041018 1103 14-15 6.661348257865072 1103 16-17 6.611922612229815 1103 18-19 6.50774372254218 1103 20-21 5.823275638191149 1103 22-23 6.062327002402874 1103 24-25 5.810248065974964 1103 26-27 5.972811891310844 1103 28-29 5.934614561595048 1103 30-31 6.047958423129426 1103 32-33 5.937913880986873 1103 34-35 5.915328802756843 1103 36-37 5.886793549691035 1103 38-39 5.744327819322095 1103 40-41 6.0265487541881875 1103 42-43 5.9661582372680115 1103 44-45 5.739576210237683 1103 46-47 5.722178964065048 1103 48-49 5.399333306091567 1103 50-51 5.366850333073344 1103 52-53 5.101652929908525 1103 54-55 5.502565920444397 1103 56-57 5.558523480752513 1103 58-59 5.6380464303509825 1103 60-61 5.381073282154656 1103 62-63 5.339640627580504 1103 64-65 5.340915330454909 1103 66-67 5.445638001272364 1103 68-69 5.353515214101364 1103 70-71 5.231183726181452 1103 72-73 5.108165625657222 1103 74-75 5.1591065587084906 1103 76-77 5.158316775052036 1103 78-79 5.066714483941798 1103 80-81 5.113320789469505 1103 82-83 4.869011641825104 1103 84-85 4.8712919589845605 1103 86-87 4.830286330602288 1103 88-89 4.863059470229793 1103 90-91 4.865923750772996 1103 92-93 4.786968532635246 1103 94-95 4.786894225128961 1103 96-97 4.803992623352354 1103 98-99 4.56474437076659 1103 100-101 4.604068169179055 1104 1 4.721293877283912 1104 2 5.559125759812918 1104 3 5.209579959587501 1104 4 6.70352726062594 1104 5 6.247649232263466 1104 6 5.685108091458883 1104 7 6.373075027574632 1104 8 5.822684236993162 1104 9 6.442511912684214 1104 10-11 6.4546890880803645 1104 12-13 6.454334787802548 1104 14-15 6.52422003358005 1104 16-17 6.428757969369908 1104 18-19 6.632826474447825 1104 20-21 5.926843115669918 1104 22-23 6.082015490521414 1104 24-25 5.946685208235298 1104 26-27 6.070284241951246 1104 28-29 5.9876998952496265 1104 30-31 6.127068802843244 1104 32-33 6.018482131000962 1104 34-35 5.855341600981443 1104 36-37 5.8004553781572525 1104 38-39 5.643039155255593 1104 40-41 5.831852999374771 1104 42-43 5.902780776953001 1104 44-45 5.825160800644756 1104 46-47 5.824043714018902 1104 48-49 5.626806662464649 1104 50-51 5.530083730471475 1104 52-53 5.234998217829897 1104 54-55 5.629334103483316 1104 56-57 5.556800638574067 1104 58-59 5.548303577407886 1104 60-61 5.345775878160676 1104 62-63 5.297747496071562 1104 64-65 5.316450662064863 1104 66-67 5.3132440701506845 1104 68-69 5.2957061419505465 1104 70-71 5.067438455059136 1104 72-73 5.02277002457722 1104 74-75 5.01187193674793 1104 76-77 4.953877515227081 1104 78-79 4.894918378006093 1104 80-81 4.922462623196473 1104 82-83 4.662377453324272 1104 84-85 4.600496943881989 1104 86-87 4.553001466215946 1104 88-89 4.656778448626433 1104 90-91 4.599659450856883 1104 92-93 4.616825585971132 1104 94-95 4.571895159023587 1104 96-97 4.557330219076551 1104 98-99 4.404514853728262 1104 100-101 4.57760485576512 1105 1 4.565544861954979 1105 2 5.287545495814623 1105 3 4.969241046451458 1105 4 6.311497108401113 1105 5 3.8279538149758388 1105 6 4.924880153413323 1105 7 5.670024543222919 1105 8 1.2099846415066793 1105 9 0.9226355288221768 1105 10-11 2.4181138805010605 1105 12-13 5.44093594279509 1105 14-15 3.708471284378433 1105 16-17 5.8936178533383945 1105 18-19 6.375320020860428 1105 20-21 3.4419245506284 1105 22-23 5.409253953902294 1105 24-25 5.618067872701982 1105 26-27 5.736665043526724 1105 28-29 3.3625069529460276 1105 30-31 5.508511079828555 1105 32-33 5.576807924060365 1105 34-35 5.582235429489007 1105 36-37 5.812350603833217 1105 38-39 5.6151273897669824 1105 40-41 5.696265789453538 1105 42-43 3.0058192855755266 1105 44-45 5.127241583591324 1105 46-47 5.4786769905784105 1105 48-49 5.281360433488704 1105 50-51 5.4154942983871734 1105 52-53 5.214943196007454 1105 54-55 5.439360117428393 1105 56-57 5.418546500294312 1105 58-59 5.402046990756837 1105 60-61 5.286274800345051 1105 62-63 5.033900891856103 1105 64-65 5.097007733067635 1105 66-67 5.105033044496015 1105 68-69 5.114333058067217 1105 70-71 5.004719565342295 1105 72-73 4.930235561089887 1105 74-75 5.037060554482654 1105 76-77 4.919646223977342 1105 78-79 4.906592052692066 1105 80-81 4.9057694546846555 1105 82-83 4.772114386473092 1105 84-85 4.694326449417439 1105 86-87 4.595144057231487 1105 88-89 4.543969740940675 1105 90-91 4.5784687307163665 1105 92-93 4.5874963598693625 1105 94-95 4.570257478057012 1105 96-97 4.527478052800209 1105 98-99 4.351163645510251 1105 100-101 4.440767512780237 1106 1 4.236317266230291 1106 2 4.5004918195167605 1106 3 4.632371625497733 1106 4 6.166721591605253 1106 5 6.000489491508606 1106 6 5.091808888437992 1106 7 5.768838995889116 1106 8 5.40863392503968 1106 9 5.936056805542872 1106 10-11 5.8649998917766055 1106 12-13 6.008015899337263 1106 14-15 5.7501548350009415 1106 16-17 5.814519456580118 1106 18-19 6.030692422199394 1106 20-21 5.3709032915257495 1106 22-23 5.316770617632621 1106 24-25 5.449977815149712 1106 26-27 5.6677395972030205 1106 28-29 5.65211964968082 1106 30-31 5.82077103402165 1106 32-33 5.514191938624609 1106 34-35 5.127259434069696 1106 36-37 5.286311873506698 1106 38-39 5.223469899163277 1106 40-41 5.496648247757509 1106 42-43 2.285253893280487 1106 44-45 4.567901452630554 1106 46-47 4.810081148436058 1106 48-49 4.71035936172456 1106 50-51 4.892412095544785 1106 52-53 4.669955161593549 1106 54-55 4.913301933848402 1106 56-57 4.880836184931383 1106 58-59 4.752729322798174 1106 60-61 4.7556285852875 1106 62-63 4.766362132166183 1106 64-65 4.71367696903193 1106 66-67 4.7777966544325885 1106 68-69 4.73858355724504 1106 70-71 4.757013581081079 1106 72-73 4.541137457965618 1106 74-75 4.635581006091769 1106 76-77 4.523301233373633 1106 78-79 4.6887552544773605 1106 80-81 4.622194240331826 1106 82-83 4.713655524598533 1106 84-85 4.719260748806679 1106 86-87 4.771842730005741 1106 88-89 4.781064526000577 1106 90-91 4.8199052617241165 1106 92-93 4.800048479906945 1106 94-95 4.793813899832912 1106 96-97 4.826888582515966 1106 98-99 4.754362054013889 1106 100-101 4.841337162768493 1107 1 2.89460349180046 1107 2 0.06407678295851937 1107 3 0.8376566315517202 1107 4 2.629663230290614 1107 5 2.624709475226595 1107 6 3.8154792725038504 1107 7 4.835863779640231 1107 8 4.362718432388931 1107 9 4.76092142180941 1107 10-11 2.518544989354247 1107 12-13 4.7565511176350235 1107 14-15 2.9385064392804736 1107 16-17 1.4856246063386678 1107 18-19 2.2262966058264944 1107 20-21 1.8880524790053812 1107 22-23 3.9686112805960576 1107 24-25 4.048647245617129 1107 26-27 3.01512194222693 1107 28-29 3.7834908588545417 1107 30-31 3.1196836048467667 1107 32-33 3.792493432668156 1107 34-35 4.344200737088411 1107 36-37 4.610811288814617 1107 38-39 4.583917469230968 1107 40-41 2.9694734166534147 1107 42-43 3.5129912929755562 1107 44-45 4.100110302678015 1107 46-47 4.381454395585887 1107 48-49 1.9079851195628628 1107 50-51 3.598103824958237 1107 52-53 3.851848833732234 1107 54-55 4.489222864734856 1107 56-57 2.9046347326281996 1107 58-59 1.8893789445737887 1107 60-61 3.0445140957133248 1107 62-63 3.8117425198611663 1107 64-65 4.003834961222232 1107 66-67 4.09231707070283 1107 68-69 3.883185263695836 1107 70-71 3.816031080429905 1107 72-73 3.8436268948194225 1107 74-75 3.931798162799055 1107 76-77 3.929025058186152 1107 78-79 3.7220618870542452 1107 80-81 3.8480711422158116 1107 82-83 3.6692191993637024 1107 84-85 3.6733761527455293 1107 86-87 3.6833598560888916 1107 88-89 3.8923636867249662 1107 90-91 3.8678343728504885 1107 92-93 3.8268960905161933 1107 94-95 4.001497832625471 1107 96-97 4.029996768531966 1107 98-99 4.071922651108196 1107 100-101 4.186554675849138 1108 1 -1.6913019428626477 1108 2 -1.1007726534079723 1108 3 -1.565571753020496 1108 4 -3.562919125015881 1108 5 -8.37599870547789 1108 6 -6.57144764817734 1108 7 -5.19013641785795 1108 8 -5.695991759288653 1108 9 -7.020948335547587 1108 10-11 -2.943912057436947 1108 12-13 -1.6375357927569105 1108 14-15 -6.279693971170964 1108 16-17 -5.610790801604725 1108 18-19 -3.782781051941818 1108 20-21 -4.268511333852839 1108 22-23 -3.3810353624867666 1108 24-25 -7.8157489278782215 1108 26-27 -8.76950560659225 1108 28-29 -5.99804688681407 1108 30-31 -5.55040921173897 1108 32-33 -2.2999658870156168 1108 34-35 -0.8655221469476437 1108 36-37 -4.524093808468358 1108 38-39 -5.3158946957801305 1108 40-41 -3.061342122310208 1108 42-43 -3.7809461932536337 1108 44-45 -1.6152109592718737 1108 46-47 -2.4675186970254863 1108 48-49 -1.3921519344991644 1108 50-51 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56-57 -4.738973470452759 2313 58-59 -4.797720954787721 2313 60-61 -3.227903023687354 2313 62-63 -5.640480296262705 2313 64-65 -5.537914352890962 2313 66-67 -6.21309673723216 2313 68-69 -6.466818494588047 2313 70-71 -6.955983067420984 2313 72-73 -7.614934893455569 2313 74-75 -8.682439051727965 2313 76-77 -7.02408408655371 2313 78-79 -7.343559837496954 2313 80-81 -8.492774931003911 2313 82-83 -8.003552242689825 2313 84-85 -7.766166631140617 2313 86-87 -6.8157976619404295 2313 88-89 -8.391370043037721 2313 90-91 -8.647010848491554 2313 92-93 -8.051846011867811 2313 94-95 -8.700775093950188 2313 96-97 -8.66803556106118 2313 98-99 -8.227855818936195 2313 100-101 -8.413980462017243 2314 1 -15.049057410772292 2314 2 -16.483590867821743 2314 3 -15.438429408093997 2314 4 -25.097877891598884 2314 5 -20.42858027593325 2314 6 -18.85222211931395 2314 7 -19.18594136586799 2314 8 -15.616378994856959 2314 9 -18.057534925723278 2314 10-11 -16.65131793768076 2314 12-13 -16.998779966295814 2314 14-15 -19.24919917016702 2314 16-17 -20.380984419388874 2314 18-19 -18.455276570048664 2314 20-21 -18.766939085735235 2314 22-23 -19.155490364279522 2314 24-25 -19.347670763661654 2314 26-27 -18.87759632010964 2314 28-29 -19.643061228872153 2314 30-31 -20.11950028380781 2314 32-33 -17.72126025775782 2314 34-35 -18.949213434147357 2314 36-37 -18.2377062143486 2314 38-39 -17.507628292051194 2314 40-41 -19.46879102872828 2314 42-43 -19.28514662351538 2314 44-45 -16.989605007317767 2314 46-47 -16.433368463967042 2314 48-49 -16.306565702874927 2314 50-51 -15.565443201612833 2314 52-53 -15.748210213083457 2314 54-55 -15.913054655298883 2314 56-57 -14.631993272432958 2314 58-59 -14.023265509243167 2314 60-61 -14.363299063291315 2314 62-63 -15.573599108143895 2314 64-65 -13.967963857841456 2314 66-67 -15.127552182776714 2314 68-69 -17.745382851023695 2314 70-71 -17.90420088920316 2314 72-73 -16.46493489345557 2314 74-75 -15.03001330915371 2314 76-77 -15.392796957840838 2314 78-79 -15.38920340185339 2314 80-81 -15.27619077258807 2314 82-83 -15.199987886254181 2314 84-85 -15.25854286876438 2314 86-87 -15.236787760950332 2314 88-89 -14.619439349968413 2314 90-91 -14.211218769283633 2314 92-93 -14.36585591285791 2314 94-95 -14.486418658306624 2314 96-97 -14.176896947199793 2314 98-99 -13.842756809035205 2314 100-101 -13.20650521449249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84815.0 3 13223.0 4 8647.0 5 7287.0 6 7310.0 7 8399.0 8 10128.0 9 11329.0 10 12572.0 11 13925.0 12 15644.0 13 16530.0 14 18064.0 15 20593.0 16 22574.0 17 23448.0 18 21715.0 19 19615.0 20 20056.0 21 21598.0 22 25758.0 23 32063.0 24 38889.0 25 46638.0 26 55257.0 27 62870.0 28 69746.0 29 77105.0 30 87347.0 31 96882.0 32 111761.0 33 133237.0 34 160917.0 35 195406.0 36 241131.0 37 297679.0 38 285862.0 39 78429.0 40 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.836209928802745 20.215006812457297 14.53750085913779 23.411282399602168 2 14.378294719678836 20.646016779319147 41.6676275419889 23.30806095901312 3 17.800750276918702 25.695064926877702 30.941184124971254 25.563000671232338 4 11.270964000361143 17.70102218184214 40.49814995416571 30.529863863631 5 12.28702278671973 37.55020333033862 36.26644332874722 13.896330554194428 6 29.28216930724707 38.35510109978117 19.03507246259571 13.327657130376044 7 25.579739605139935 32.51606482970371 23.47708246106921 18.42711310408715 8 23.94914997117689 36.29304503484057 21.681651311198536 18.076153682784007 9 24.597848639390424 16.684634450329426 22.068092573797024 36.64942433648312 10-11 23.065788288447855 27.035999809722956 30.016892148339174 19.881319753490015 12-13 24.06512802056034 24.655335499536175 29.6388659370517 21.640670542851783 14-15 21.272399189359298 26.400549329048545 27.478915107499724 24.848136374092437 16-17 20.72752865131853 29.36983687582284 28.22503837573343 21.6775960971252 18-19 20.525854229578187 28.468817152034738 30.14276484610602 20.862563772281057 20-21 21.44409118374162 27.74553658574576 30.10865446915921 20.701717761353414 22-23 21.09732580304279 27.6773570500407 29.814024744790903 21.411292402125603 24-25 20.97097479746919 27.755569875405172 29.955021918673776 21.318433408451867 26-27 20.9110988012764 27.87791389484408 29.913242670387973 21.29774463349155 28-29 20.857854683654605 27.946481072029105 29.910083718645964 21.285580525670326 30-31 21.10081813983287 27.728432518686454 29.941898983409214 21.228850358071462 32-33 20.9663606162649 27.871184550981344 29.781214956446885 21.381239876306875 34-35 21.2498267501773 28.006579952390442 29.73043845104036 21.013154846391902 36-37 21.08799288480436 27.732005087947563 29.707317576503833 21.472684450744246 38-39 21.030353939117326 27.982206339456884 29.774560762502215 21.212878958923575 40-41 21.216272059428857 27.83682631853929 29.626318377478654 21.320583244553195 42-43 21.151220605409925 27.904224536217683 29.59145408333539 21.353100775037003 44-45 21.255433283365598 28.065168659308764 29.4089784982336 21.270419559092037 46-47 21.278079378215036 28.029587852284006 29.197234551842243 21.49509821765871 48-49 21.228945088353573 28.12859746641286 29.28178896265956 21.360668482574006 50-51 21.109878456578116 28.278703774106866 29.083182745460928 21.52823502385409 52-53 21.094403002808054 28.380095475867623 29.03833494616969 21.487166575154635 54-55 21.140990671202815 28.24353083350779 28.995026843775527 21.620451651513864 56-57 21.157842545835482 28.350924006992486 28.85624539861383 21.634988048558203 58-59 21.15158298617524 28.410307518989203 28.92931638862596 21.5087931062096 60-61 21.3221829874249 28.403510731823417 28.80396066374268 21.470345617009013 62-63 21.17798648887294 28.34392716583561 28.90732184762258 21.570764497668872 64-65 21.06437000945221 28.305859684557173 28.924213787298154 21.705556518692468 66-67 21.11684413306161 28.294281835951786 28.885499724596723 21.70337430638988 68-69 21.206698745187513 28.28995782268555 28.732461984043177 21.770881448083752 70-71 21.243574091967318 28.168158047130127 28.71534778200346 21.872920078899096 72-73 21.256970450824856 28.256739702079525 28.7358969478528 21.75039289924281 74-75 21.193827733736153 28.34730151263683 28.69952674280971 21.75934401081731 76-77 21.30657536936974 28.262356878191735 28.62084586589982 21.8102218865387 78-79 21.295623896517878 28.32521671590788 28.510866406983403 21.86829298059084 80-81 21.30083695924072 28.374740811774895 28.442268159138234 21.88215406984615 82-83 21.399844739635803 28.343058322048464 28.56018844615573 21.69690849216 84-85 21.529666165970177 28.320892702641647 28.372518188133373 21.7769229432548 86-87 21.36630332831829 28.280562186382753 28.501389948002732 21.85174453729623 88-89 21.54581049587542 28.25354720630352 28.39819959402769 21.802442703793375 90-91 21.473676255873283 28.271025110393676 28.419476163977897 21.835822469755144 92-93 21.541062163550347 28.1944456231085 28.459725857558922 21.804766355782238 94-95 21.453591820421707 28.25724484381624 28.408767159159652 21.880396176602403 96-97 21.404734511647153 28.193536367982418 28.471728025223143 21.930001095147286 98-99 21.496468251033622 28.15078117138732 28.26761035225352 22.085140225325546 100-101 21.488123309543177 28.131020026647235 28.256113326326005 22.124743337483576 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 126.5 2 92.5 3 129.5 4 126.5 5 133.5 6 216.5 7 400.5 8 700.0 9 949.5 10 990.0 11 1024.0 12 1169.0 13 1320.0 14 1554.5 15 1778.0 16 1985.5 17 2227.5 18 2508.0 19 2882.0 20 3419.0 21 4209.5 22 5297.0 23 6853.5 24 8923.0 25 11887.0 26 16175.0 27 21459.0 28 27394.0 29 33980.0 30 40952.0 31 47992.0 32 55471.0 33 62779.5 34 69371.5 35 75156.5 36 81189.0 37 86594.0 38 89873.5 39 92278.5 40 94198.5 41 95032.5 42 95459.5 43 95909.5 44 95484.0 45 94947.0 46 93468.0 47 91082.5 48 89033.5 49 86280.0 50 81642.0 51 76500.5 52 71422.5 53 65719.0 54 60032.5 55 53969.5 56 47811.5 57 41803.5 58 36122.0 59 30850.0 60 25536.0 61 20705.0 62 16466.5 63 12741.0 64 9639.5 65 7172.0 66 5392.0 67 4191.5 68 3283.0 69 2537.5 70 1981.5 71 1548.5 72 1155.5 73 863.0 74 686.5 75 534.5 76 409.5 77 292.5 78 215.5 79 164.0 80 125.5 81 100.0 82 78.5 83 65.5 84 47.5 85 39.5 86 39.5 87 30.0 88 22.0 89 19.0 90 14.0 91 9.0 92 5.0 93 3.5 94 4.5 95 3.5 96 2.0 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04619016040472764 2 0.4655790358905225 3 0.0 4 0.6342559646125987 5 0.4574563567642318 6 0.0906426332351742 7 0.10256397822152122 8 0.104180431778992 9 0.10248315554364769 10-11 0.18065889071682845 12-13 0.34917417408315765 14-15 0.12828579545477506 16-17 0.11876892513516582 18-19 0.23470905654475777 20-21 0.11389935879328508 22-23 0.09357245530808998 24-25 0.09941189378445318 26-27 0.23784093531235745 28-29 0.10678696314041364 30-31 0.11757679063653112 32-33 0.10624141006476726 34-35 0.1378228714438527 36-37 0.13022553972374 38-39 0.1257196754322902 40-41 0.10597873636167825 42-43 0.10272562357726829 44-45 0.09258237750413913 46-47 0.08837959825471509 48-49 0.09403718570586284 50-51 0.08167131599121134 52-53 0.08892515133036148 54-55 0.2993469931741208 56-57 0.3194516342951636 58-59 0.3320801777129041 60-61 0.15990766817279728 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2474553.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.57676853028389 #Duplication Level Percentage of deduplicated Percentage of total 1 88.6603351003465 71.43963299198023 2 7.8950746577808175 12.723192064586305 3 1.72009704744602 4.15799584925048 4 0.6486155840544633 2.090533911259655 5 0.3067393571288351 1.2358033089249114 6 0.18433578223713698 0.8911908994302361 7 0.12097611589121202 0.6823505141501293 8 0.08467198124086733 0.5458075706756725 9 0.06236040843438826 0.4522320176284499 >10 0.3002891001292751 4.1388348671264374 >50 0.01237638735980048 0.6781637659187149 >100 0.003874668866747217 0.4826384619711726 >500 1.0152363355452633E-4 0.05386823902367673 >1k 1.522854503084007E-4 0.4277555380738476 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4717 0.19062028576474216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3629 0.14665274900153683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.041133893676959E-5 0.0 8 0.0 0.0 0.0 4.041133893676959E-5 4.041133893676959E-5 9 0.0 0.0 0.0 4.041133893676959E-5 4.041133893676959E-5 10-11 0.0 0.0 0.0 1.6164535574707836E-4 4.041133893676959E-5 12-13 0.0 0.0 0.0 2.0205669468384795E-4 4.041133893676959E-5 14-15 0.0 0.0 0.0 2.0205669468384795E-4 4.041133893676959E-5 16-17 0.0 0.0 0.0 2.4246803362061754E-4 4.041133893676959E-5 18-19 0.0 4.041133893676959E-5 0.0 2.4246803362061754E-4 8.082267787353918E-5 20-21 0.0 4.041133893676959E-5 0.0 2.4246803362061754E-4 1.2123401681030877E-4 22-23 0.0 4.041133893676959E-5 0.0 3.637020504309263E-4 1.2123401681030877E-4 24-25 0.0 4.041133893676959E-5 0.0 4.445247283044655E-4 1.2123401681030877E-4 26-27 0.0 4.041133893676959E-5 0.0 8.688437871405462E-4 1.2123401681030877E-4 28-29 0.0 4.041133893676959E-5 0.0 0.0022024179720539425 1.2123401681030877E-4 30-31 0.0 4.041133893676959E-5 0.0 0.007435686364365604 1.2123401681030877E-4 32-33 0.0 4.041133893676959E-5 0.0 0.020710811205094416 1.2123401681030877E-4 34-35 0.0 4.041133893676959E-5 0.0 0.04220964351945584 1.2123401681030877E-4 36-37 0.0 4.041133893676959E-5 0.0 0.07819594084264916 1.2123401681030877E-4 38-39 0.0 4.041133893676959E-5 0.0 0.12804332742115446 1.2123401681030877E-4 40-41 6.0617008405154386E-5 4.041133893676959E-5 0.0 0.17760783462710233 1.2123401681030877E-4 42-43 8.082267787353918E-5 4.041133893676959E-5 0.0 0.23236519888642515 1.2123401681030877E-4 44-45 8.082267787353918E-5 4.041133893676959E-5 0.0 0.29987234058029877 1.2123401681030877E-4 46-47 8.082267787353918E-5 4.041133893676959E-5 0.0 0.38075563546224306 1.4143968627869358E-4 48-49 8.082267787353918E-5 4.041133893676959E-5 0.0 0.46305332720697434 1.6164535574707836E-4 50-51 8.082267787353918E-5 4.041133893676959E-5 0.0 0.5476342596016331 1.6164535574707836E-4 52-53 8.082267787353918E-5 4.041133893676959E-5 0.0 0.6314069652175565 2.0205669468384795E-4 54-55 8.082267787353918E-5 4.041133893676959E-5 0.0 0.7254037395844826 2.2226236415223276E-4 56-57 1.0102834734192398E-4 4.041133893676959E-5 0.0 0.8402527648427817 2.626737030890023E-4 58-59 1.2123401681030877E-4 4.041133893676959E-5 0.0 0.965144007826868 2.828793725573871E-4 60-61 1.6164535574707836E-4 4.041133893676959E-5 0.0 1.0961171573209385 3.030850420257719E-4 62-63 1.6164535574707836E-4 4.041133893676959E-5 0.0 1.2301817742436714 3.232907114941567E-4 64-65 1.6164535574707836E-4 4.041133893676959E-5 0.0 1.370469737362667 3.232907114941567E-4 66-67 1.6164535574707836E-4 4.041133893676959E-5 0.0 1.5350045038437243 3.232907114941567E-4 68-69 1.6164535574707836E-4 4.041133893676959E-5 0.0 1.714147969350424 3.637020504309263E-4 70-71 1.6164535574707836E-4 4.041133893676959E-5 0.0 1.910486459574719 3.637020504309263E-4 72-73 2.0205669468384795E-4 4.041133893676959E-5 0.0 2.1084009920175486 3.637020504309263E-4 74-75 2.0205669468384795E-4 4.041133893676959E-5 0.0 2.3129429840460074 3.839077198993111E-4 76-77 2.4246803362061754E-4 4.041133893676959E-5 0.0 2.532093675100109 4.041133893676959E-4 78-79 2.4246803362061754E-4 4.041133893676959E-5 0.0 2.777430913785237 4.041133893676959E-4 80-81 2.4246803362061754E-4 4.041133893676959E-5 0.0 3.0432769069807764 4.041133893676959E-4 82-83 2.4246803362061754E-4 4.041133893676959E-5 0.0 3.3148613103053357 4.041133893676959E-4 84-85 2.828793725573871E-4 4.041133893676959E-5 0.0 3.596993073092393 4.041133893676959E-4 86-87 2.828793725573871E-4 4.041133893676959E-5 0.0 3.893329421515724 4.041133893676959E-4 88-89 2.828793725573871E-4 4.041133893676959E-5 0.0 4.212498176438331 4.041133893676959E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2880 0.0 49.723396 1 GTATCAA 5385 0.0 41.585052 1 ATCAACG 6055 0.0 35.96023 3 TCAACGC 6280 0.0 35.447613 4 CAACGCA 6295 0.0 34.92348 5 AACGCAG 6695 0.0 33.31387 6 TATCAAC 6695 0.0 32.80419 2 ACGCAGA 7345 0.0 29.659248 7 CGCAGAG 7275 0.0 29.223862 8 GCAGAGT 8295 0.0 24.48049 9 GTGGTAT 1625 0.0 22.770596 1 GTACATG 5245 0.0 21.530762 1 GAGTACT 5555 0.0 21.400702 12-13 TGGTATC 1560 0.0 21.256327 2 AGAGTAC 7610 0.0 20.326235 10-11 TAGGCCG 165 6.1367566E-5 20.231424 5 TACATGG 5275 0.0 20.134161 2 CAGAGTA 8480 0.0 19.955353 10-11 GTACTTT 6405 0.0 18.75923 14-15 ACATGGG 5150 0.0 18.6773 3 >>END_MODULE