FastQCFastQC Report
Sat 18 Jun 2016
SRR1931112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474553
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63010.2546318466405852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.20528960179878952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32980.1332765958134661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.051.875921
GTATCAA57550.041.1387671
ATCAACG68300.033.650493
TCAACGC69350.033.0711944
CAACGCA69550.033.0443655
AACGCAG72800.031.7648476
TATCAAC74300.031.5082932
ACGCAGA84300.027.2062577
CGCAGAG83900.026.8831928
GTGGTAT15800.022.8677671
GCAGAGT96300.022.7312779
TGGTATC15200.021.8686122
GAGTACT66250.020.6780312-13
GTACATG58250.020.485431
AGAGTAC89250.020.24396910-11
TACATGG57900.019.683372
CAGAGTA96250.019.65969710-11
ACATGGG57850.019.3720423
GTACTTT71050.019.24764414-15
GTGTAGG12300.018.552541
Produced by FastQC (version 0.11.5)