##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931112_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474553 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.247978927911426 31.0 31.0 34.0 30.0 34.0 2 31.26840201038329 31.0 31.0 34.0 28.0 34.0 3 31.424425744770875 31.0 31.0 34.0 28.0 34.0 4 35.02017293628385 35.0 35.0 37.0 32.0 37.0 5 34.90589047799744 35.0 35.0 37.0 32.0 37.0 6 35.03342462254799 36.0 35.0 37.0 32.0 37.0 7 34.888094536669854 36.0 35.0 37.0 32.0 37.0 8 34.949811945834256 36.0 35.0 37.0 32.0 37.0 9 36.33164777638628 38.0 35.0 39.0 32.0 39.0 10-11 36.20832287689939 38.0 35.0 39.0 31.5 39.0 12-13 36.14588311505149 38.0 35.0 39.0 31.5 39.0 14-15 37.08521922949316 39.0 36.0 40.0 31.5 41.0 16-17 37.1416217393606 39.0 36.0 40.0 31.0 41.0 18-19 37.22924503940712 39.0 36.0 40.0 31.5 41.0 20-21 37.21039638270023 39.0 36.0 40.0 31.0 41.0 22-23 37.1041232497344 39.0 36.0 40.0 31.0 41.0 24-25 36.98685338321709 39.0 36.0 40.0 31.0 41.0 26-27 36.75397475826947 39.0 36.0 40.0 30.5 41.0 28-29 36.47404500934108 38.0 35.0 40.0 30.0 41.0 30-31 36.245710235343516 38.0 35.0 40.0 30.0 41.0 32-33 36.08683608716402 38.0 35.0 40.0 29.5 41.0 34-35 35.859975114697484 38.0 34.0 40.0 29.0 41.0 36-37 36.10392846708072 38.0 35.0 40.0 30.0 41.0 38-39 36.11809708662534 38.0 35.0 40.0 30.0 41.0 40-41 35.96112651456647 38.0 35.0 40.0 29.0 41.0 42-43 35.80307695975799 38.0 35.0 40.0 28.5 41.0 44-45 35.57949839829658 38.0 34.0 40.0 27.5 41.0 46-47 35.391731153060775 38.0 34.0 40.0 27.0 41.0 48-49 35.153090679407555 38.0 34.0 40.0 26.5 41.0 50-51 34.854806706504164 38.0 33.0 40.0 26.0 41.0 52-53 34.74083784020791 38.0 33.0 40.0 26.0 41.0 54-55 34.51393302143862 38.0 33.0 40.0 24.5 41.0 56-57 34.3132511205054 38.0 33.0 40.0 24.0 41.0 58-59 34.0967000908851 37.0 33.0 40.0 24.0 41.0 60-61 33.83683396556873 37.0 32.5 40.0 23.0 41.0 62-63 33.60184061525456 37.0 32.0 40.0 22.5 41.0 64-65 33.37918706934141 36.5 32.0 39.5 22.5 41.0 66-67 33.15867310176828 36.0 31.5 39.0 22.0 41.0 68-69 32.83780646444025 36.0 31.0 39.0 22.0 40.0 70-71 32.04025090592119 35.0 31.0 39.0 19.0 40.0 72-73 32.00461537901997 35.0 31.0 38.0 20.0 40.0 74-75 31.704268003150467 35.0 30.5 37.5 20.0 39.5 76-77 30.963219013696616 34.0 30.0 36.5 18.5 39.0 78-79 30.973375797568288 34.0 30.0 36.0 18.0 39.0 80-81 30.702143781119254 34.0 30.0 36.0 17.5 38.5 82-83 30.334717017578527 34.0 30.0 36.0 14.5 37.5 84-85 29.96281409208047 34.0 29.0 35.0 11.0 37.0 86-87 29.622209142418853 34.0 29.0 35.0 7.5 36.5 88-89 29.30108589308857 34.0 29.0 35.0 6.5 36.0 90-91 28.992598461217035 34.0 29.0 35.0 2.0 36.0 92-93 28.65067347516905 34.0 29.0 35.0 2.0 35.5 94-95 28.271705435284677 33.5 29.0 35.0 2.0 35.0 96-97 27.908663706131975 33.0 28.5 35.0 2.0 35.0 98-99 27.436226057797107 33.0 27.0 35.0 2.0 35.0 100-101 26.76260500381281 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7941041779046891 1101 2 0.7260092785195944 1101 3 0.5083194341894703 1101 4 0.8449068017767303 1101 5 0.7697137403478393 1101 6 0.8443154925873984 1101 7 0.796678934292018 1101 8 0.7740975705521507 1101 9 1.0820460987957716 1101 10-11 0.8808130373822252 1101 12-13 1.1499813853925644 1101 14-15 1.3839564085113167 1101 16-17 1.1883624232688206 1101 18-19 0.9737635049053708 1101 20-21 0.8337114971070037 1101 22-23 0.7832034689628955 1101 24-25 0.6780366772073023 1101 26-27 0.9509991186316498 1101 28-29 0.9060048497410378 1101 30-31 1.100237835392896 1101 32-33 1.1253277255563319 1101 34-35 0.8883493969265075 1101 36-37 1.121397522314446 1101 38-39 0.9521740437738515 1101 40-41 1.1125167976602697 1101 42-43 1.0407567580913835 1101 44-45 0.8777997296569495 1101 46-47 0.9017435748985676 1101 48-49 0.9020082579060755 1101 50-51 0.6552812445172407 1101 52-53 0.5864114475227638 1101 54-55 0.6356647569068983 1101 56-57 0.4678896782645623 1101 58-59 0.49848902255605765 1101 60-61 0.6761602326565495 1101 62-63 0.5803568382138877 1101 64-65 0.6313324543934513 1101 66-67 0.3498654298252646 1101 68-69 0.3033632668959285 1101 70-71 0.6781637226524353 1101 72-73 0.05059166577745344 1101 74-75 -0.15044033402858759 1101 76-77 0.025137293369134284 1101 78-79 0.03354916645616868 1101 80-81 -0.09086186477231806 1101 82-83 -0.08602291260496742 1101 84-85 -0.007261290712403223 1101 86-87 -0.12829290076814104 1101 88-89 -0.21076043736076144 1101 90-91 -0.0825417963727979 1101 92-93 -0.29197746345165143 1101 94-95 -0.20104478240462242 1101 96-97 -0.2438719004781511 1101 98-99 -0.3036021450149171 1101 100-101 -0.54960790181336 1102 1 0.8812657037921845 1102 2 0.8781036426146862 1102 3 0.6346182508626512 1102 4 0.9660223449344016 1102 5 0.9413267782640489 1102 6 0.9238458042323359 1102 7 1.020193557084383 1102 8 0.9624206578624808 1102 9 1.413445569319208 1102 10-11 1.2927073819328783 1102 12-13 1.4513383911848337 1102 14-15 1.7260473523299424 1102 16-17 1.5257620359336173 1102 18-19 1.2916733162127159 1102 20-21 1.3465495270277543 1102 22-23 0.9657951501398259 1102 24-25 0.739359124881517 1102 26-27 1.0976272590673162 1102 28-29 1.28124782262757 1102 30-31 1.6120333472794641 1102 32-33 1.664596719195501 1102 34-35 1.5881099775739642 1102 36-37 1.7867729975996127 1102 38-39 1.5864529873082063 1102 40-41 1.5936350948067854 1102 42-43 1.7155864105557512 1102 44-45 1.7278228276278824 1102 46-47 1.6394610067595536 1102 48-49 1.4869186200111955 1102 50-51 1.2626639334764036 1102 52-53 1.2370277635740834 1102 54-55 1.1178029183084064 1102 56-57 1.2839281097346529 1102 58-59 1.2483504347304688 1102 60-61 1.5212408090397602 1102 62-63 1.391326153447892 1102 64-65 1.4871751305598977 1102 66-67 1.1874895814190225 1102 68-69 1.0615641570561891 1102 70-71 1.496713392173774 1102 72-73 0.9931794380669956 1102 74-75 0.8834912515582758 1102 76-77 0.960184022341096 1102 78-79 1.0141046726570835 1102 80-81 0.8456473655322192 1102 82-83 0.9151897308689705 1102 84-85 0.7566814440873557 1102 86-87 0.6617878532917665 1102 88-89 0.5554734384787565 1102 90-91 0.7178193315188928 1102 92-93 0.652863471483144 1102 94-95 0.4970984960858331 1102 96-97 0.573932637384754 1102 98-99 0.6195931039101374 1102 100-101 0.4493244833186516 1103 1 0.682169104492349 1103 2 0.5424732471523015 1103 3 0.41777471859888493 1103 4 0.761191594903444 1103 5 0.6664817450052993 1103 6 0.6882403954467122 1103 7 0.7115728844448412 1103 8 0.6877605574133483 1103 9 0.971846767423628 1103 10-11 0.8434282610153048 1103 12-13 1.0664862611715762 1103 14-15 1.3981559295338428 1103 16-17 1.1600853859606204 1103 18-19 0.9752648302528044 1103 20-21 0.94532383408486 1103 22-23 0.7866036687573654 1103 24-25 0.5060572454527659 1103 26-27 0.9184381328024998 1103 28-29 0.7831020852559405 1103 30-31 1.1953681759255943 1103 32-33 1.2739278411107477 1103 34-35 1.0941195649214563 1103 36-37 1.4147021471374686 1103 38-39 1.167779293120006 1103 40-41 1.257215959229157 1103 42-43 1.3254288172975848 1103 44-45 1.1996846670061743 1103 46-47 1.2831185291399052 1103 48-49 1.0636827825672412 1103 50-51 0.9749203133779645 1103 52-53 0.5930980568826811 1103 54-55 0.8138514346291501 1103 56-57 0.9515145806828187 1103 58-59 1.0087320176207442 1103 60-61 1.1096822056379807 1103 62-63 0.9567512095669741 1103 64-65 0.8784459094264534 1103 66-67 0.8366724340990146 1103 68-69 0.7732107857837818 1103 70-71 1.2285156784771303 1103 72-73 0.526550557954593 1103 74-75 0.3763999362452566 1103 76-77 0.5968640600947523 1103 78-79 0.6175656638341422 1103 80-81 0.4071485152782266 1103 82-83 0.43747912503401665 1103 84-85 0.44055602083907175 1103 86-87 0.47018355612167895 1103 88-89 0.31749945422978243 1103 90-91 0.45510248125771824 1103 92-93 0.269681624550536 1103 94-95 0.19752192380929046 1103 96-97 -0.01537869481333587 1103 98-99 -0.023534124479748897 1103 100-101 -0.0799474421537596 1104 1 0.5407986776748714 1104 2 0.4264650775084107 1104 3 0.26071552515228547 1104 4 0.6481417160887091 1104 5 0.42971724228485186 1104 6 0.628086750439401 1104 7 0.5528248337426476 1104 8 0.6191685942121126 1104 9 0.892862159554376 1104 10-11 0.742264097812523 1104 12-13 0.91980672628425 1104 14-15 1.1935068473478054 1104 16-17 0.9503152456111863 1104 18-19 0.8420228233408835 1104 20-21 0.8193874803852523 1104 22-23 0.5674685546509153 1104 24-25 0.5656942643727021 1104 26-27 0.3275022156571836 1104 28-29 0.7401878587804092 1104 30-31 0.9247478378004743 1104 32-33 1.0478473692342405 1104 34-35 0.9081416335698975 1104 36-37 1.169553260019363 1104 38-39 0.7345948671667983 1104 40-41 0.9437666608658546 1104 42-43 0.9822667495554072 1104 44-45 0.9563905830352226 1104 46-47 1.01119305945722 1104 48-49 0.8211047581577731 1104 50-51 0.552060009646695 1104 52-53 0.3809435011899467 1104 54-55 0.7007843699428591 1104 56-57 0.6843055551714698 1104 58-59 0.5648830123566739 1104 60-61 0.8128372774594581 1104 62-63 0.013760392679955658 1104 64-65 -0.8556605855067474 1104 66-67 -0.31612720516346826 1104 68-69 0.26080242074039717 1104 70-71 0.7944061442418757 1104 72-73 0.3806303512378548 1104 74-75 0.198859825747288 1104 76-77 0.03167114177903585 1104 78-79 0.025361473576040794 1104 80-81 0.0837488183242705 1104 82-83 -0.11092524139307613 1104 84-85 -0.2537623631806092 1104 86-87 -0.027643674477349833 1104 88-89 0.019258823292783944 1104 90-91 0.10570045011979445 1104 92-93 0.17250961221207461 1104 94-95 0.31606382141686495 1104 96-97 0.3501146170643672 1104 98-99 0.18959671028942182 1104 100-101 -0.12715774969797167 1105 1 -0.29189040578265235 1105 2 -1.7825373442501586 1105 3 -0.3298682251138416 1105 4 0.4303597541981432 1105 5 0.5556047768814025 1105 6 -0.0495911556708819 1105 7 -0.2060292220376354 1105 8 -0.3044681686152231 1105 9 0.5530767912341688 1105 10-11 0.9226022790984487 1105 12-13 1.0604130973721766 1105 14-15 1.2698394798792023 1105 16-17 0.8214391012637279 1105 18-19 0.5871197602154865 1105 20-21 1.0514870918393697 1105 22-23 0.3463300619960279 1105 24-25 -0.09962236060068363 1105 26-27 1.0184096432705587 1105 28-29 0.5298883990188656 1105 30-31 0.948955443719143 1105 32-33 1.1283319205435873 1105 34-35 0.6674620774702618 1105 36-37 0.8922782041326229 1105 38-39 0.7400165458979018 1105 40-41 1.0239388452388596 1105 42-43 1.305336993860287 1105 44-45 1.2972042007699542 1105 46-47 1.3871261683565166 1105 48-49 1.2416946958839858 1105 50-51 0.9450951251459543 1105 52-53 1.0256279407258262 1105 54-55 0.12348203600630114 1105 56-57 0.48331023901221926 1105 58-59 -0.361687804121928 1105 60-61 0.3025621018154254 1105 62-63 0.8336583993863655 1105 64-65 1.3584684463220782 1105 66-67 0.9160785245576264 1105 68-69 0.9017025298525994 1105 70-71 0.4888144766881197 1105 72-73 -0.15021679853860448 1105 74-75 -0.23527333105492687 1105 76-77 0.12112152234109885 1105 78-79 -0.4833741555489297 1105 80-81 -0.48030536519712896 1105 82-83 -0.35414897462705497 1105 84-85 -0.27189993769625787 1105 86-87 -0.6790920723909117 1105 88-89 -1.0429051909716378 1105 90-91 -1.1402480941637911 1105 92-93 -0.6584985445901381 1105 94-95 -0.7893731596348701 1105 96-97 -0.8455751184050833 1105 98-99 -1.4763599508772778 1105 100-101 -0.897186491448025 1106 1 0.2687310706091708 1106 2 0.33603427308599265 1106 3 -0.3673953862601884 1106 4 0.3935725507684964 1106 5 -0.01948184515289597 1106 6 0.3548669427552369 1106 7 0.461914752592385 1106 8 0.5822588398414368 1106 9 0.9186269145604555 1106 10-11 0.19715338609868382 1106 12-13 0.3828812380815947 1106 14-15 0.08821876693581032 1106 16-17 -0.09662966782249072 1106 18-19 0.4505487448290566 1106 20-21 0.43365836856006723 1106 22-23 0.4825028362502053 1106 24-25 0.2716319705010335 1106 26-27 0.7380526577173541 1106 28-29 0.6611519795920415 1106 30-31 0.23976396497825192 1106 32-33 0.2894140352957635 1106 34-35 -0.9874834534200403 1106 36-37 -0.8299913665744398 1106 38-39 -0.6540498447527909 1106 40-41 0.4686960099088111 1106 42-43 0.5962229610907244 1106 44-45 0.44521593144883553 1106 46-47 0.014488086961165436 1106 48-49 -0.09367021778761853 1106 50-51 0.48242812080016506 1106 52-53 -0.2504808947216759 1106 54-55 0.3051713859005929 1106 56-57 -0.12605668605237952 1106 58-59 0.43598147588745917 1106 60-61 0.4890931095673636 1106 62-63 0.38047577371100516 1106 64-65 0.4550090705879981 1106 66-67 0.314250285774456 1106 68-69 0.32206421355003556 1106 70-71 0.6431664998264779 1106 72-73 -0.014815914700218258 1106 74-75 0.09451547268010074 1106 76-77 0.1276904822894167 1106 78-79 0.1498490650289419 1106 80-81 0.1950792953619498 1106 82-83 0.22698724219230115 1106 84-85 0.28267598137820826 1106 86-87 0.18273036712048096 1106 88-89 0.17902559949112629 1106 90-91 0.37329026912021135 1106 92-93 -0.0862275057129942 1106 94-95 0.1615400421504276 1106 96-97 0.3859006127436153 1106 98-99 0.2693496891274201 1106 100-101 0.0531952327709071 1107 1 0.3647893850612576 1107 2 0.34517475938850595 1107 3 0.18651714642099293 1107 4 -1.2344614997798544 1107 5 0.028142495910579157 1107 6 0.22135237122608942 1107 7 0.39758020628183033 1107 8 0.5304996959221739 1107 9 0.841909538253077 1107 10-11 0.7208179940825374 1107 12-13 0.9932702299591227 1107 14-15 1.023569805595244 1107 16-17 0.8778856044652841 1107 18-19 0.6545967429807149 1107 20-21 0.08673892746683975 1107 22-23 0.4231211167868736 1107 24-25 0.2103726429459556 1107 26-27 0.7499084474766846 1107 28-29 0.7134660633147121 1107 30-31 0.813273218152645 1107 32-33 1.045205374878158 1107 34-35 0.8798943797209375 1107 36-37 1.107315911179903 1107 38-39 0.9490982289238588 1107 40-41 0.3232748082003951 1107 42-43 -0.06770845828878436 1107 44-45 -0.32604506354122975 1107 46-47 0.11822657430337102 1107 48-49 0.5176887744277536 1107 50-51 -0.9216766028361008 1107 52-53 0.3877154637310838 1107 54-55 1.0560134405728476 1107 56-57 0.9428737143390862 1107 58-59 0.8207142558067488 1107 60-61 1.3094526543249074 1107 62-63 1.3544494565735903 1107 64-65 1.1947160379596724 1107 66-67 0.9413945472369747 1107 68-69 0.9634536010346864 1107 70-71 1.4639487339060153 1107 72-73 0.9577507472801869 1107 74-75 0.5698802649536603 1107 76-77 0.5372084845933074 1107 78-79 0.659512668192491 1107 80-81 0.7971077899733032 1107 82-83 0.8690344491297033 1107 84-85 0.6307928151698086 1107 86-87 0.6520159420640539 1107 88-89 0.8665445761001749 1107 90-91 0.9164246872671384 1107 92-93 0.8906620632278148 1107 94-95 0.8530710526269303 1107 96-97 1.0028813130009233 1107 98-99 0.9178843625117743 1107 100-101 0.8082302870497315 1108 1 0.4488120087354197 1108 2 0.4716842935150858 1108 3 0.2597135123938976 1108 4 0.48944995026059246 1108 5 0.4532381335749065 1108 6 0.46825156654952593 1108 7 0.3896799764368879 1108 8 0.4469848815167836 1108 9 0.6672453796822992 1108 10-11 0.6796648768852123 1108 12-13 0.7270670992953185 1108 14-15 0.8880093802996853 1108 16-17 0.7945825788868532 1108 18-19 0.6200965623439743 1108 20-21 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0.8867126095555591 2311 76-77 1.126059934749854 2311 78-79 1.1921027853933381 2311 80-81 1.2963618544445445 2311 82-83 1.2972714799184004 2311 84-85 1.322419280950058 2311 86-87 1.2233195898519504 2311 88-89 1.2783145336468067 2311 90-91 1.3314496082250642 2311 92-93 1.1994134909108567 2311 94-95 1.1822675172065367 2311 96-97 1.3757099263858912 2311 98-99 1.538807807915024 2311 100-101 1.3306005025798413 2312 1 0.02937923407251475 2312 2 0.00720103348163903 2312 3 -0.2402025875863636 2312 4 0.4005827433356046 2312 5 -0.8376990831987783 2312 6 0.22647772122036258 2312 7 0.38767147626832354 2312 8 -2.199110044342625 2312 9 -4.529299469596037 2312 10-11 -0.40679830120673444 2312 12-13 -5.784358094274012 2312 14-15 -1.9477794099436352 2312 16-17 -2.7016947083832434 2312 18-19 -2.1979299051795778 2312 20-21 -3.7303743299891536 2312 22-23 -1.7265087457112003 2312 24-25 -2.0211698276251298 2312 26-27 -2.396122031047298 2312 28-29 -5.293482826572756 2312 30-31 -5.862190376143779 2312 32-33 -1.3441874364330033 2312 34-35 0.7149448622885757 2312 36-37 1.2707040656999382 2312 38-39 1.320857988416975 2312 40-41 -2.2762672520067113 2312 42-43 -4.460325386522491 2312 44-45 -3.486753553335827 2312 46-47 0.2612660154404409 2312 48-49 -1.2291532528422522 2312 50-51 -6.539094993501404 2312 52-53 -1.4057313716367759 2312 54-55 0.8182813134536033 2312 56-57 1.1499176358827796 2312 58-59 1.25878169348573 2312 60-61 1.822316724859526 2312 62-63 1.6578641167664188 2312 64-65 1.6509735785475996 2312 66-67 1.592518578547029 2312 68-69 1.3833168370425568 2312 70-71 1.7883111548854558 2312 72-73 1.432678948584119 2312 74-75 1.1591311287278216 2312 76-77 1.4127089156554575 2312 78-79 1.0494664302521812 2312 80-81 1.1215002083189702 2312 82-83 0.39321742586434283 2312 84-85 0.4142016318757058 2312 86-87 0.9226524801760085 2312 88-89 1.073291223744512 2312 90-91 1.311987656838408 2312 92-93 1.5782289948512158 2312 94-95 1.7866823815522501 2312 96-97 1.8014100021820134 2312 98-99 1.9186231976420522 2312 100-101 1.7917020382299782 2313 1 -0.12068484772684585 2313 2 0.0363336053448009 2313 3 -0.630157941585491 2313 4 -1.235411510428321 2313 5 -1.814157824378725 2313 6 -1.7240314121965312 2313 7 -1.6696993390493375 2313 8 -3.8761800273010962 2313 9 -1.937401944139367 2313 10-11 -1.4082947168511453 2313 12-13 -3.9817758090184583 2313 14-15 -2.7975463212009046 2313 16-17 -5.795690736720076 2313 18-19 -3.760589229433485 2313 20-21 -3.5890422735971654 2313 22-23 -2.5281861396241325 2313 24-25 -1.4923471018248051 2313 26-27 -2.965823311274896 2313 28-29 -4.683209317002742 2313 30-31 -3.9204836876939915 2313 32-33 -8.098975966495118 2313 34-35 -3.971846540891576 2313 36-37 -5.051993566794465 2313 38-39 -3.06874526778347 2313 40-41 -1.5791945931049725 2313 42-43 -3.1419303891280137 2313 44-45 -5.213662979698093 2313 46-47 -2.6297209851088326 2313 48-49 -1.592296359471547 2313 50-51 0.15500595997943378 2313 52-53 -0.09808239905815697 2313 54-55 -3.431796148062105 2313 56-57 -5.030303622373921 2313 58-59 -1.2685763054720667 2313 60-61 -7.796819086303387 2313 62-63 -2.43840646334991 2313 64-65 -2.583530597332288 2313 66-67 -3.2118734893937635 2313 68-69 -4.94642275142553 2313 70-71 -4.153884949504501 2313 72-73 -0.7086002931380619 2313 74-75 1.3573023327614564 2313 76-77 -1.5484793439955382 2313 78-79 -0.2272366668000565 2313 80-81 0.7483962549648844 2313 82-83 1.056182371007509 2313 84-85 1.6566174452920386 2313 86-87 1.610824106726998 2313 88-89 -2.503737211673709 2313 90-91 0.8122763770333279 2313 92-93 1.4428333073450545 2313 94-95 1.9277398016612608 2313 96-97 2.3179445148082394 2313 98-99 2.0341500063684457 2313 100-101 2.063187045905707 2314 1 -28.74444722396447 2314 2 -14.878616889704706 2314 3 -4.9548114069320235 2314 4 -4.626401609438222 2314 5 -2.301781586754963 2314 6 -1.3560116102163349 2314 7 -0.937620131128547 2314 8 -4.841229532251589 2314 9 -3.6264118451294642 2314 10-11 -1.3237897663561 2314 12-13 -0.23543917535509706 2314 14-15 -2.5690809746662495 2314 16-17 -1.9754927169180903 2314 18-19 -4.172965467057246 2314 20-21 -2.78993336270608 2314 22-23 -6.572988119822149 2314 24-25 -1.4367530424188644 2314 26-27 -4.055823311274896 2314 28-29 -4.2351400100720475 2314 30-31 -6.37553319264449 2314 32-33 -3.8855601249109597 2314 34-35 -3.117935649802469 2314 36-37 -6.831102477685555 2314 38-39 -8.98651754501119 2314 40-41 -7.81617479112478 2314 42-43 -6.1940095970488045 2314 44-45 -6.340494662866412 2314 46-47 -8.018186331643484 2314 48-49 -7.337197349570559 2314 50-51 -3.1142514657631395 2314 52-53 -3.738775468365084 2314 54-55 -5.830410009448244 2314 56-57 -3.5766897609877795 2314 58-59 -2.206744622303752 2314 60-61 -2.139937898184577 2314 62-63 -0.47796091879545344 2314 64-65 -3.2076395082233766 2314 66-67 -2.136279429987823 2314 68-69 -0.43815542469285873 2314 70-71 -0.03235029603915507 2314 72-73 0.17907297418867074 2314 74-75 -0.2766085583276521 2314 76-77 -2.7198159776589037 2314 78-79 -3.84030597373075 2314 80-81 -3.374623547015311 2314 82-83 -2.887728520081602 2314 84-85 -0.6013033467871658 2314 86-87 0.6944874730636315 2314 88-89 0.3910152635738129 2314 90-91 0.7380189512907549 2314 92-93 0.8970412281371338 2314 94-95 1.138581385819677 2314 96-97 1.243885108867648 2314 98-99 0.6504866400318114 2314 100-101 1.1691771449156079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 14.0 4 43.0 5 106.0 6 193.0 7 597.0 8 1364.0 9 2617.0 10 4040.0 11 5913.0 12 7843.0 13 10001.0 14 12272.0 15 14523.0 16 16680.0 17 18676.0 18 17074.0 19 14621.0 20 14090.0 21 15245.0 22 16550.0 23 19356.0 24 22793.0 25 28286.0 26 36084.0 27 45286.0 28 58190.0 29 72464.0 30 89121.0 31 108714.0 32 132006.0 33 159414.0 34 194085.0 35 238207.0 36 297743.0 37 372409.0 38 346870.0 39 80987.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.71401995678175 21.504579346272376 15.525434316715089 23.255966380230785 2 14.625025206572662 22.064833527509816 40.630691684518375 22.679449581399144 3 18.03688250974357 26.33237581492791 31.40756013856805 24.223181536760464 4 12.464473266802583 18.214500039401184 39.26103586719983 30.059990826596405 5 11.938836457273375 38.443536713401166 36.611160188576 13.006466640749453 6 29.40183540219183 39.481554850512396 18.19556905833094 12.921040688964835 7 25.467629911341565 33.37507824645501 23.321706991121225 17.835584851082196 8 24.005224377897747 37.215327374277294 20.967988966088015 17.811459281736944 9 24.470924647805077 16.70527970102075 21.626168443351183 37.19762720782299 10-11 23.188672863341377 27.59096289309625 29.704576947836642 19.515787295725733 12-13 24.005264789236683 25.063718578668553 29.671338621561144 21.25967801053362 14-15 21.15119377115786 26.957393921245576 27.375509839554862 24.515902468041702 16-17 20.66645976061131 29.963290339709836 28.053046348168742 21.31720355151011 18-19 20.545326772148343 28.85389805754817 30.129037446358996 20.471737723944486 20-21 21.32459882653554 27.979073392244985 30.205354259941085 20.490973521278388 22-23 21.016179487770113 28.014554547831466 29.88153820104075 21.087727763357663 24-25 20.97570627075179 28.034664849930284 30.066832158901647 20.922796720416276 26-27 21.016194422794445 28.101236713878592 29.908325029770573 20.97424383355639 28-29 20.892621335092684 28.165826226384404 30.015347170371776 20.926205268151136 30-31 21.204798632772366 27.95279767015229 29.97478476934601 20.867618927729335 32-33 21.106179303394 28.07977607029062 29.786890542493683 21.027154083821692 34-35 21.295535367345252 28.074129200729136 29.844916206174616 20.785419225750996 36-37 21.174282881993822 27.990889557300534 29.74908699575635 21.085740564949283 38-39 21.101992231676515 28.114436784424075 29.792225736011574 20.991345247887836 40-41 21.326072860537778 28.0162877710983 29.72619256175352 20.931446806610403 42-43 21.193851978131512 28.08626078719525 29.565356021643797 21.15453121302944 44-45 21.353290451271935 28.155102829254258 29.420671890804712 21.070934828669095 46-47 21.299538069497164 28.131351342282763 29.230136715500898 21.338973872719176 48-49 21.30730665105966 28.21900633037765 29.225169374585203 21.24851764397749 50-51 21.388680255666365 28.302252713007103 29.04109744175897 21.267969589567556 52-53 21.380830529420276 28.350490883477104 28.944202466904667 21.324476120197954 54-55 21.365819168499637 28.297980371698277 28.94812693512845 21.388073524673633 56-57 21.407287414380086 28.387148005891227 28.836850201442576 21.368714378286107 58-59 21.330758827821388 28.414616084207022 28.933464079202416 21.321161008769174 60-61 21.38474949302111 28.409488608028738 28.89038102019752 21.315380878752634 62-63 21.352322515424063 28.321778293984366 28.847286571239845 21.478612619351725 64-65 21.250270782342728 28.305848251958526 28.85755717144806 21.586323794250685 66-67 21.24095747690368 28.30034917273543 28.79895086525327 21.65974248510762 68-69 21.340110967356587 28.294372444823956 28.746562705406703 21.61895388241275 70-71 21.352370263963028 28.06641237122985 28.704542097466536 21.87667526734059 72-73 21.29771243791288 28.154619629076645 28.767928504415032 21.779739428595445 74-75 21.265013937977816 28.265443109692573 28.617832690922818 21.851710261406794 76-77 21.154062976222374 28.275187478304165 28.536083082479948 22.034666462993517 78-79 21.198717505747503 28.29735309771098 28.45980668023679 22.044122716304724 80-81 21.196911189109755 28.29003479226177 28.377808521932018 22.135245496696463 82-83 21.242694356633603 28.293815927590654 28.5100781255525 21.953411590223244 84-85 21.31850102190194 28.21435390843395 28.38070721877996 22.08643785088415 86-87 21.15706433689591 28.230958638055608 28.428633258719564 22.183343766328917 88-89 21.23967049025567 28.160785527694078 28.35135205038344 22.248191931666817 90-91 21.20763183619994 28.152751493314486 28.37822874575323 22.261387924732343 92-93 21.197692124903618 28.078823706275735 28.41822780022017 22.305256368600485 94-95 21.03918088869608 28.12020680244838 28.41389828515291 22.42671402370263 96-97 20.999287343301905 28.07434987503756 28.41922156551299 22.507141216147545 98-99 20.995812306464405 28.04037158834316 28.31559751668258 22.64821858850986 100-101 20.98178852641601 28.028570805064813 28.286826667701227 22.70281400081795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 151.5 2 114.5 3 150.0 4 134.5 5 127.0 6 223.0 7 454.0 8 818.0 9 1071.5 10 1107.0 11 1168.0 12 1300.0 13 1459.0 14 1665.5 15 1896.5 16 2100.5 17 2320.5 18 2631.5 19 3020.5 20 3623.0 21 4364.0 22 5427.0 23 7086.0 24 9220.5 25 12294.5 26 16932.5 27 22413.0 28 28103.5 29 34595.5 30 41603.0 31 48718.0 32 56334.5 33 63371.0 34 69847.5 35 75356.5 36 81092.5 37 86809.0 38 89757.0 39 91398.0 40 92832.5 41 93772.5 42 94304.5 43 94749.5 44 94746.0 45 94459.0 46 93219.0 47 90947.5 48 88371.0 49 85453.5 50 81641.5 51 76656.5 52 71182.0 53 65600.0 54 59916.5 55 53707.5 56 47933.5 57 42346.0 58 36735.0 59 31413.5 60 25769.5 61 20659.0 62 16369.0 63 12480.5 64 9292.0 65 6996.0 66 5238.5 67 3967.0 68 3099.5 69 2444.5 70 1901.5 71 1414.5 72 1081.5 73 850.5 74 662.5 75 523.0 76 380.5 77 277.5 78 207.0 79 151.0 80 116.0 81 84.5 82 57.5 83 41.5 84 35.5 85 28.0 86 20.5 87 20.0 88 13.5 89 7.0 90 4.0 91 2.0 92 2.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11767781898387306 2 0.0 3 0.004081545232613728 4 3.232907114941567E-4 5 2.8287937255738716E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.020993690577651804 26-27 0.008627820863000308 28-29 0.006283963204667672 30-31 0.002283240649927482 32-33 0.0010709004818243942 34-35 0.004707920986133657 36-37 0.0012123401681030878 38-39 6.667870924566983E-4 40-41 0.002364063327801021 42-43 0.0013537798543817813 44-45 5.657587451147743E-4 46-47 0.036875346779802254 48-49 0.018225513860483086 50-51 0.08534874783445738 52-53 0.0014143968627869357 54-55 0.03556197826435724 56-57 0.0010709004818243942 58-59 0.0013133685154450116 60-61 0.003677431843246033 62-63 0.003697637512714418 64-65 0.009739132683761472 66-67 0.005374708078590355 68-69 0.0016770705658759378 70-71 0.00543532508699551 72-73 0.004303807596765962 74-75 7.678154397986223E-4 76-77 0.0 78-79 0.0 80-81 3.434963809625415E-4 82-83 4.243190588360807E-4 84-85 2.0205669468384795E-5 86-87 0.001293162845976627 88-89 0.0035966091653724932 90-91 9.698721344824702E-4 92-93 0.0027883823866371015 94-95 7.071984313934679E-4 96-97 3.839077198993111E-4 98-99 0.0016366592269391686 100-101 0.0027883823866371015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2474553.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.86852617953843 #Duplication Level Percentage of deduplicated Percentage of total 1 86.4515307491365 66.45401754651171 2 9.292900827513375 14.286631810871322 3 2.119506238349156 4.887699623107113 4 0.8075755389789796 2.4830856583984224 5 0.3834775764182478 1.4738678061086015 6 0.230329025659026 1.062303164326705 7 0.13906622695131696 0.7482871134967859 8 0.10034175534525465 0.6170498278126039 9 0.07360948473265753 0.5092427343811159 >10 0.37802003480741614 5.1057521821519805 >50 0.017013615920509362 0.8924803236843486 >100 0.006364802727837667 0.8010632654655014 >500 5.2824691948143566E-5 0.03163173292218761 >1k 1.0564938389628694E-4 0.18475530782535732 >5k 1.0564938389628694E-4 0.4621319029363498 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6301 0.2546318466405852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5080 0.20528960179878952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3298 0.1332765958134661 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.041133893676959E-5 0.0 0.0 0.0 0.0 7 4.041133893676959E-5 0.0 0.0 4.041133893676959E-5 0.0 8 4.041133893676959E-5 0.0 0.0 4.041133893676959E-5 0.0 9 4.041133893676959E-5 0.0 0.0 8.082267787353918E-5 0.0 10-11 4.041133893676959E-5 0.0 0.0 8.082267787353918E-5 0.0 12-13 4.041133893676959E-5 0.0 0.0 1.2123401681030877E-4 0.0 14-15 4.041133893676959E-5 0.0 0.0 1.2123401681030877E-4 0.0 16-17 4.041133893676959E-5 0.0 0.0 1.6164535574707836E-4 0.0 18-19 4.041133893676959E-5 0.0 0.0 1.8185102521546314E-4 0.0 20-21 4.041133893676959E-5 0.0 0.0 2.0205669468384795E-4 0.0 22-23 8.082267787353918E-5 0.0 0.0 2.4246803362061754E-4 0.0 24-25 8.082267787353918E-5 0.0 0.0 3.030850420257719E-4 0.0 26-27 8.082267787353918E-5 0.0 0.0 7.476097703302374E-4 0.0 28-29 1.0102834734192398E-4 0.0 0.0 0.0022024179720539425 2.0205669468384795E-5 30-31 1.2123401681030877E-4 0.0 0.0 0.007839799753733301 4.041133893676959E-5 32-33 1.2123401681030877E-4 0.0 0.0 0.021902945703729117 4.041133893676959E-5 34-35 1.2123401681030877E-4 0.0 0.0 0.04566481299854964 4.041133893676959E-5 36-37 1.2123401681030877E-4 0.0 0.0 0.0842576416831646 4.041133893676959E-5 38-39 1.2123401681030877E-4 0.0 0.0 0.13695402765671214 6.0617008405154386E-5 40-41 1.6164535574707836E-4 0.0 0.0 0.189246300240892 8.082267787353918E-5 42-43 1.8185102521546314E-4 0.0 0.0 0.24727698295409312 8.082267787353918E-5 44-45 2.0205669468384795E-4 0.0 0.0 0.3185625848385547 1.0102834734192398E-4 46-47 2.0205669468384795E-4 0.0 0.0 0.4038709213340753 1.2123401681030877E-4 48-49 2.0205669468384795E-4 0.0 0.0 0.490916945403877 1.2123401681030877E-4 50-51 2.0205669468384795E-4 0.0 0.0 0.5806503235129739 1.2123401681030877E-4 52-53 2.0205669468384795E-4 0.0 0.0 0.6689693047592837 1.2123401681030877E-4 54-55 2.2226236415223276E-4 0.0 0.0 0.7676133831039383 1.2123401681030877E-4 56-57 2.4246803362061754E-4 0.0 0.0 0.8858771665023946 1.6164535574707836E-4 58-59 2.4246803362061754E-4 0.0 0.0 1.013758848567802 2.0205669468384795E-4 60-61 2.4246803362061754E-4 2.0205669468384795E-5 0.0 1.144610764045062 2.0205669468384795E-4 62-63 2.4246803362061754E-4 4.041133893676959E-5 0.0 1.2795038134159988 2.0205669468384795E-4 64-65 2.828793725573871E-4 4.041133893676959E-5 0.0 1.4185188193584861 2.0205669468384795E-4 66-67 2.828793725573871E-4 4.041133893676959E-5 0.0 1.5812754869263257 2.0205669468384795E-4 68-69 2.828793725573871E-4 8.082267787353918E-5 0.0 1.758256945799908 2.0205669468384795E-4 70-71 2.828793725573871E-4 8.082267787353918E-5 0.0 1.9536659752286574 2.0205669468384795E-4 72-73 3.232907114941567E-4 8.082267787353918E-5 0.0 2.1557024642430367 2.0205669468384795E-4 74-75 3.232907114941567E-4 8.082267787353918E-5 0.0 2.363053044327602 2.2226236415223276E-4 76-77 3.232907114941567E-4 8.082267787353918E-5 0.0 2.5850931461156823 2.4246803362061754E-4 78-79 3.232907114941567E-4 8.082267787353918E-5 0.0 2.835825298548869 2.4246803362061754E-4 80-81 3.232907114941567E-4 8.082267787353918E-5 0.0 3.104762759173071 2.626737030890023E-4 82-83 3.232907114941567E-4 8.082267787353918E-5 0.0 3.3791759562232047 3.232907114941567E-4 84-85 3.232907114941567E-4 8.082267787353918E-5 0.0 3.663308080287632 3.232907114941567E-4 86-87 3.232907114941567E-4 8.082267787353918E-5 0.0 3.96180643534408 3.434963809625415E-4 88-89 3.232907114941567E-4 8.082267787353918E-5 0.0 4.284874884474085 3.637020504309263E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3290 0.0 51.87592 1 GTATCAA 5755 0.0 41.138767 1 ATCAACG 6830 0.0 33.65049 3 TCAACGC 6935 0.0 33.071194 4 CAACGCA 6955 0.0 33.044365 5 AACGCAG 7280 0.0 31.764847 6 TATCAAC 7430 0.0 31.508293 2 ACGCAGA 8430 0.0 27.206257 7 CGCAGAG 8390 0.0 26.883192 8 GTGGTAT 1580 0.0 22.867767 1 GCAGAGT 9630 0.0 22.731277 9 TGGTATC 1520 0.0 21.868612 2 GAGTACT 6625 0.0 20.67803 12-13 GTACATG 5825 0.0 20.48543 1 AGAGTAC 8925 0.0 20.243969 10-11 TACATGG 5790 0.0 19.68337 2 CAGAGTA 9625 0.0 19.659697 10-11 ACATGGG 5785 0.0 19.372042 3 GTACTTT 7105 0.0 19.247644 14-15 GTGTAGG 1230 0.0 18.55254 1 >>END_MODULE