FastQCFastQC Report
Sat 18 Jun 2016
SRR1931111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481429
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68780.2771789964572833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.218341931201739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.12404142935381185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.056.984581
GTATCAA66600.042.902631
ATCAACG81100.033.468133
TATCAAC84400.033.1705132
TCAACGC84150.032.6618774
CAACGCA83800.032.446195
AACGCAG87650.031.8185256
ACGCAGA97600.028.0916527
CGCAGAG96450.027.5870598
GTGGTAT16750.023.5489041
GCAGAGT107650.023.5230399
GAGTACT73100.022.51530612-13
TGGTATC16000.022.2476122
GTACATG67150.021.2756141
TACATGG62750.021.0043132
CAGAGTA106550.020.7275110-11
AGAGTAC99200.020.63190810-11
GTACCGT2601.4593752E-820.1350926
ACATGGG63100.019.69013
GTGTAGC18600.019.6549661
Produced by FastQC (version 0.11.5)