FastQCFastQC Report
Sat 18 Jun 2016
SRR1931111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481429
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87930.35435227040548006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74070.29849735777247705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.1914219588793393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.061.7840731
GTATCAA81200.040.3242381
ATCAACG93650.034.281633
CAACGCA96700.033.1970185
TCAACGC97550.032.9077534
AACGCAG100350.031.8949076
TATCAAC102100.031.7700232
GTGGTAT17400.029.5398461
ACGCAGA114100.027.8432087
TGGTATC18100.027.8131282
CGCAGAG115050.027.1592678
GCAGAGT130800.023.453289
GAGTACT94200.022.28179712-13
CAGAGTA128400.021.67261910-11
GTACTTT101550.020.71584314-15
TACATGG67900.020.0740362
GTACATG71100.019.9471281
AGAGTAC123050.019.8555810-11
ACATGGG66450.019.7973633
GTACTAG7550.018.9107191
Produced by FastQC (version 0.11.5)