##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931111_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2481429 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.269097765843792 31.0 31.0 34.0 30.0 34.0 2 31.30888250278368 31.0 31.0 34.0 28.0 34.0 3 31.466581554418845 31.0 31.0 34.0 28.0 34.0 4 35.057688936495865 35.0 35.0 37.0 32.0 37.0 5 34.9626094480237 35.0 35.0 37.0 32.0 37.0 6 35.08383959404037 36.0 35.0 37.0 32.0 37.0 7 34.9423175114017 36.0 35.0 37.0 32.0 37.0 8 34.99638272946758 36.0 35.0 37.0 32.0 37.0 9 36.38117995719402 38.0 35.0 39.0 32.0 39.0 10-11 36.276030263207204 38.0 35.0 39.0 32.0 39.0 12-13 36.19404927563916 38.0 35.0 39.0 32.0 39.0 14-15 37.12186788338494 39.0 36.0 41.0 31.5 41.0 16-17 37.184952501159614 39.0 36.0 41.0 32.0 41.0 18-19 37.298385124055535 39.0 36.0 41.0 32.0 41.0 20-21 37.289526720289 39.0 36.0 41.0 31.5 41.0 22-23 37.19445730665677 39.0 36.0 40.0 31.0 41.0 24-25 37.07393965332073 39.0 36.0 40.0 31.0 41.0 26-27 36.843803509993634 39.0 36.0 40.0 30.5 41.0 28-29 36.5640705416113 39.0 35.0 40.0 30.0 41.0 30-31 36.33726594635591 38.0 35.0 40.0 30.0 41.0 32-33 36.180072248692184 38.0 35.0 40.0 30.0 41.0 34-35 35.94737669302648 38.0 34.5 40.0 29.5 41.0 36-37 36.12059422211959 38.0 35.0 40.0 30.0 41.0 38-39 36.085157181607855 38.0 35.0 40.0 30.0 41.0 40-41 35.93155435839591 38.0 35.0 40.0 29.0 41.0 42-43 35.753060434128884 38.0 35.0 40.0 28.5 41.0 44-45 35.50673845594615 38.0 34.0 40.0 27.0 41.0 46-47 35.30252850272967 38.0 34.0 40.0 27.0 41.0 48-49 35.02893272384581 38.0 34.0 40.0 26.0 41.0 50-51 34.70258790398597 38.0 33.0 40.0 24.0 41.0 52-53 34.59620605707437 38.0 33.0 40.0 24.0 41.0 54-55 34.377216716658026 38.0 33.0 40.0 24.0 41.0 56-57 34.177182180106705 38.0 33.0 40.0 24.0 41.0 58-59 33.954783513854316 37.5 33.0 40.0 23.0 41.0 60-61 33.684950889185224 37.0 32.5 40.0 23.0 41.0 62-63 33.457004008577314 37.0 32.0 40.0 22.5 41.0 64-65 33.23678291822978 36.5 32.0 39.5 22.0 41.0 66-67 33.01694205234162 36.0 32.0 39.0 22.0 41.0 68-69 32.70584348776451 36.0 31.0 39.0 21.0 40.5 70-71 31.90862301520616 35.0 31.0 39.0 16.0 40.0 72-73 31.883691413294518 35.0 31.0 38.0 19.0 40.0 74-75 31.57465718342133 35.0 30.5 37.5 18.0 40.0 76-77 30.841135289383658 34.0 30.0 37.0 18.0 39.0 78-79 30.845174091219214 34.0 30.0 36.5 16.0 39.0 80-81 30.567266885330994 34.0 30.0 36.0 13.0 39.0 82-83 30.1999694530853 34.0 30.0 36.0 9.5 37.5 84-85 29.841512692887846 34.0 29.0 35.0 7.0 37.0 86-87 29.51004703338278 34.0 29.0 35.0 6.0 36.5 88-89 29.188343087793363 34.0 29.0 35.0 2.0 36.0 90-91 28.878635858612114 34.0 29.0 35.0 2.0 36.0 92-93 28.559192908602263 34.0 29.0 35.0 2.0 35.5 94-95 28.190235344231084 34.0 29.0 35.0 2.0 35.0 96-97 27.847298068975576 33.5 28.5 35.0 2.0 35.0 98-99 27.40017909035479 33.0 27.0 35.0 2.0 35.0 100-101 26.76072557385281 33.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8304861137522437 1101 2 0.7568328809740485 1101 3 0.5266125622449351 1101 4 0.7863277245796212 1101 5 0.7138869169419095 1101 6 0.7503881874006169 1101 7 0.7737547901583284 1101 8 0.6944758760067984 1101 9 1.0007241846014807 1101 10-11 0.8562638837155063 1101 12-13 1.001783036419873 1101 14-15 1.3745671219243647 1101 16-17 1.059419135624033 1101 18-19 0.9477073359732202 1101 20-21 0.9240347238528841 1101 22-23 0.7793825661846583 1101 24-25 0.6844027871158787 1101 26-27 0.9402808046391229 1101 28-29 0.8739945325568854 1101 30-31 0.9735249712636289 1101 32-33 0.9642080892455169 1101 34-35 0.6571123294686316 1101 36-37 0.9103341924530497 1101 38-39 0.7970562989492791 1101 40-41 0.79660367536367 1101 42-43 0.9375888867762683 1101 44-45 0.9139911263081117 1101 46-47 0.812225813973285 1101 48-49 0.6623560612123782 1101 50-51 0.5838506070325309 1101 52-53 0.5802470875147563 1101 54-55 0.6795079398862853 1101 56-57 0.44450067668633864 1101 58-59 0.5883743088293301 1101 60-61 0.6546873261917199 1101 62-63 0.34735103020330627 1101 64-65 0.45635495894204325 1101 66-67 0.29413409426995685 1101 68-69 0.28964511109407454 1101 70-71 0.5643146087507773 1101 72-73 0.11421315159480372 1101 74-75 -0.16936724502469858 1101 76-77 -0.11078658786238904 1101 78-79 0.0813288483189325 1101 80-81 0.026833524148205612 1101 82-83 -0.04079437785859241 1101 84-85 0.024600846721281044 1101 86-87 -0.11266593138793013 1101 88-89 -0.1989428150699375 1101 90-91 -0.2610621761860159 1101 92-93 -0.3771541962381555 1101 94-95 -0.5016404698566994 1101 96-97 -0.6279019958399914 1101 98-99 -0.6274202082154403 1101 100-101 -0.7898577403381886 1102 1 1.0119904305376473 1102 2 0.9241680382012021 1102 3 0.7216469656703666 1102 4 0.9551753543798966 1102 5 1.0208427691760775 1102 6 0.8700256808957718 1102 7 1.0115370854039298 1102 8 0.9594142054701891 1102 9 1.3308373005080085 1102 10-11 1.2955511956835153 1102 12-13 1.3753394163456818 1102 14-15 1.7653092377426347 1102 16-17 1.5353161961929302 1102 18-19 1.454307285456025 1102 20-21 1.4538619307086122 1102 22-23 1.056455673083299 1102 24-25 0.7823506022472984 1102 26-27 1.2278073185572183 1102 28-29 1.3710068666642101 1102 30-31 1.5257280990690845 1102 32-33 1.6697430693581765 1102 34-35 1.6070059694758143 1102 36-37 1.7300844366701966 1102 38-39 1.5301672754831301 1102 40-41 1.5869580869448896 1102 42-43 1.628477685045297 1102 44-45 1.7801213267639824 1102 46-47 1.5799288946090755 1102 48-49 1.3882865970293707 1102 50-51 1.3758795418196996 1102 52-53 1.3702598994051627 1102 54-55 1.242939731025082 1102 56-57 1.4085452170255266 1102 58-59 1.3838991246393846 1102 60-61 1.533155437974706 1102 62-63 1.4810100878446377 1102 64-65 1.5948869475848042 1102 66-67 1.261743748531508 1102 68-69 1.2906143716867007 1102 70-71 1.58647750539442 1102 72-73 1.0738815640463812 1102 74-75 1.0358755874682082 1102 76-77 1.024627637900771 1102 78-79 1.0853839181452543 1102 80-81 1.0047436644094958 1102 82-83 1.1017424677383438 1102 84-85 1.0330617156778565 1102 86-87 0.9150903748923298 1102 88-89 0.9977554603461449 1102 90-91 1.048377691617187 1102 92-93 0.9568002043875303 1102 94-95 0.8943321534767641 1102 96-97 1.0528536926697818 1102 98-99 1.0009878002811838 1102 100-101 0.939124368663304 1103 1 0.6494780542608822 1103 2 0.4819983546297877 1103 3 0.3263454354688555 1103 4 0.7080500532233387 1103 5 0.6493284048473029 1103 6 0.5553383536995184 1103 7 0.6601237974671648 1103 8 0.6482320014932199 1103 9 0.8551568820062556 1103 10-11 0.8344356329715623 1103 12-13 0.898674693651003 1103 14-15 1.3064285205118509 1103 16-17 1.076554278334747 1103 18-19 0.8770236230081139 1103 20-21 0.9502345527738711 1103 22-23 0.8115090241626461 1103 24-25 0.5283199448056521 1103 26-27 1.0066806319838761 1103 28-29 0.7476266171931414 1103 30-31 1.0638614970895546 1103 32-33 1.1978023567165508 1103 34-35 1.0564631761087142 1103 36-37 1.311757907238814 1103 38-39 1.0977138988928132 1103 40-41 1.107994975599567 1103 42-43 1.1329725004497533 1103 44-45 1.1428918288651246 1103 46-47 1.1725011314797342 1103 48-49 0.9652366431962704 1103 50-51 0.8182429881341591 1103 52-53 0.5457082418537667 1103 54-55 0.7564132841236884 1103 56-57 0.8567730829483864 1103 58-59 1.0060458752759587 1103 60-61 1.0950473928003746 1103 62-63 0.8226443162601313 1103 64-65 0.8352971120087602 1103 66-67 0.6956915609070933 1103 68-69 0.6827630186622855 1103 70-71 1.0015948705069206 1103 72-73 0.5467057024390627 1103 74-75 0.4696648347457746 1103 76-77 0.675198770935804 1103 78-79 0.5943454962553929 1103 80-81 0.4216366365115256 1103 82-83 0.6285793836375255 1103 84-85 0.6395397748949385 1103 86-87 0.4331592118813177 1103 88-89 0.29289977830421776 1103 90-91 0.3210832766108318 1103 92-93 0.17854539733211539 1103 94-95 0.1289179275608241 1103 96-97 0.014824364890923647 1103 98-99 -0.11970012217108206 1103 100-101 -0.34767965939970225 1104 1 0.5220925674991648 1104 2 0.379608433181037 1104 3 0.15272524554825395 1104 4 0.5684961247413369 1104 5 0.3306034445614472 1104 6 0.42864023635500814 1104 7 0.4807701159696407 1104 8 0.5109718883556553 1104 9 0.770145332015943 1104 10-11 0.6170919185569232 1104 12-13 0.6519436450420244 1104 14-15 1.097362152980267 1104 16-17 0.8211251887682138 1104 18-19 0.7550942300156294 1104 20-21 0.6717931861263935 1104 22-23 0.40458856420001865 1104 24-25 0.39816110237920554 1104 26-27 0.2511077369041885 1104 28-29 0.5754763951758761 1104 30-31 0.7284575303433414 1104 32-33 0.8240607115396088 1104 34-35 0.6778697994234264 1104 36-37 0.9038205388825347 1104 38-39 0.3374208841968098 1104 40-41 0.6570421332268808 1104 42-43 0.7524372825276728 1104 44-45 0.9237209951015259 1104 46-47 0.8843177688411643 1104 48-49 0.6731624120521715 1104 50-51 0.7791113663401035 1104 52-53 0.5150844575026312 1104 54-55 0.783759841830495 1104 56-57 0.7467583855326723 1104 58-59 0.6794838681663151 1104 60-61 0.9040742937392281 1104 62-63 0.005937159792772206 1104 64-65 -0.7348865420318944 1104 66-67 -0.23753300117640208 1104 68-69 0.19902766833615715 1104 70-71 0.7131043474401793 1104 72-73 0.32616287411308775 1104 74-75 0.14710010033897092 1104 76-77 -0.022525718297170982 1104 78-79 -0.08963737609210654 1104 80-81 -0.09529628584113681 1104 82-83 -0.20325018292309593 1104 84-85 -0.24386399946931547 1104 86-87 -0.048861307917754004 1104 88-89 0.0395404073932184 1104 90-91 0.0832011190471782 1104 92-93 0.21861191056474283 1104 94-95 0.2283867270266846 1104 96-97 0.26717095796205825 1104 98-99 0.1617370559250979 1104 100-101 -0.2026865837355949 1105 1 -0.2673138486499447 1105 2 -1.6956982651505506 1105 3 -0.3205027752385199 1105 4 0.3947598419451239 1105 5 0.5851958810036777 1105 6 0.09546298173034984 1105 7 -0.027557335782489645 1105 8 -0.15346898340808224 1105 9 0.49223855229838875 1105 10-11 0.8730121469712913 1105 12-13 0.9176697145669834 1105 14-15 1.2458406023775481 1105 16-17 0.7708040144087889 1105 18-19 0.6833854916981892 1105 20-21 1.0109755573931452 1105 22-23 0.39805160363219017 1105 24-25 -0.13194536647065291 1105 26-27 0.911325978033453 1105 28-29 0.43742185323875304 1105 30-31 0.820007932937024 1105 32-33 0.8764718707559069 1105 34-35 0.6309632665321558 1105 36-37 0.877324471425581 1105 38-39 0.7407966672031492 1105 40-41 1.0137943996925287 1105 42-43 1.1706122356640378 1105 44-45 1.1520281003629336 1105 46-47 1.18832452654447 1105 48-49 1.0308044539059082 1105 50-51 0.6868762209460826 1105 52-53 0.8347864981555873 1105 54-55 0.11035584057635361 1105 56-57 0.3861704720152659 1105 58-59 -0.24203121158525676 1105 60-61 0.3373432076368772 1105 62-63 0.8823339630588904 1105 64-65 1.2804705889127774 1105 66-67 0.9514147517037941 1105 68-69 0.9981652439684012 1105 70-71 0.45692525162488096 1105 72-73 -0.2413181302697467 1105 74-75 -0.40655072719616925 1105 76-77 -0.08893669739698495 1105 78-79 -0.5218531089326781 1105 80-81 -0.42202166825509835 1105 82-83 -0.3107677442794383 1105 84-85 -0.2597636479086418 1105 86-87 -0.5877190692700971 1105 88-89 -0.8292614511419671 1105 90-91 -0.8015069588431878 1105 92-93 -0.38866179309696136 1105 94-95 -0.6502457553118965 1105 96-97 -0.7215654582537603 1105 98-99 -1.4630073454544608 1105 100-101 -0.8854024399893028 1106 1 0.42457433278260126 1106 2 0.5278883696918051 1106 3 -0.12103965682859652 1106 4 0.43257612240581267 1106 5 0.11930838403002042 1106 6 0.541757769801329 1106 7 0.5003471719560224 1106 8 0.5414617173249994 1106 9 0.90168592932487 1106 10-11 0.28186442827160363 1106 12-13 0.46350412499113247 1106 14-15 0.24659491969421055 1106 16-17 -0.07192673503855929 1106 18-19 0.5151841474888457 1106 20-21 0.58554699478902 1106 22-23 0.48794018730874456 1106 24-25 0.48275236354398743 1106 26-27 0.7434410277269805 1106 28-29 0.8282495625234816 1106 30-31 0.3442533814171469 1106 32-33 0.43580118008770086 1106 34-35 -0.9281176410170033 1106 36-37 -0.6701160241249937 1106 38-39 -0.5747520548231009 1106 40-41 0.6698994407117596 1106 42-43 0.7893187196092626 1106 44-45 0.6672759005360618 1106 46-47 0.1282998898302523 1106 48-49 0.008333204545941442 1106 50-51 0.5823545107473862 1106 52-53 0.05601467005020311 1106 54-55 0.6510602125940963 1106 56-57 0.42631356139569476 1106 58-59 0.9006528875096365 1106 60-61 1.0872817292935082 1106 62-63 0.7838054764829749 1106 64-65 0.9768287582282369 1106 66-67 0.6634741605690238 1106 68-69 0.7328277878778451 1106 70-71 1.1957105405599222 1106 72-73 0.5444454688129952 1106 74-75 0.41691757647288696 1106 76-77 0.4953082109519755 1106 78-79 0.5155272242038293 1106 80-81 0.4934288128422146 1106 82-83 0.4850689349883375 1106 84-85 0.640111514460056 1106 86-87 0.442428190391297 1106 88-89 0.541772610202294 1106 90-91 0.8517512689352422 1106 92-93 0.3469537383233039 1106 94-95 0.45214946290255753 1106 96-97 0.7826981014535654 1106 98-99 0.7866882635573305 1106 100-101 0.5066328430174671 1107 1 0.3689846454342671 1107 2 0.404214791620511 1107 3 0.1467137212186067 1107 4 -1.3491940859606117 1107 5 -0.09604210024576787 1107 6 0.2328493518809438 1107 7 0.4479556813820622 1107 8 0.4913913776017438 1107 9 0.890841988683917 1107 10-11 0.8362278217671388 1107 12-13 0.7522973526121604 1107 14-15 1.0892828593925046 1107 16-17 1.0320462689917704 1107 18-19 0.8351939282313623 1107 20-21 0.25175379917696716 1107 22-23 0.5852516234354326 1107 24-25 0.5216136602515817 1107 26-27 0.8323275014942269 1107 28-29 0.699339416118427 1107 30-31 0.9524057073293335 1107 32-33 0.9494373036567509 1107 34-35 0.8868247688347211 1107 36-37 1.1441020826321378 1107 38-39 1.018124287703337 1107 40-41 0.37533969615938645 1107 42-43 0.07704099693582123 1107 44-45 0.15669858465668796 1107 46-47 0.37781755458345145 1107 48-49 0.583281119941411 1107 50-51 -0.7381582610338242 1107 52-53 0.3911964550227083 1107 54-55 0.7410946582526634 1107 56-57 0.7061732340479168 1107 58-59 0.8656031595659215 1107 60-61 1.1844885860961512 1107 62-63 1.2019204659260154 1107 64-65 1.103297920181248 1107 66-67 0.8867334624772809 1107 68-69 1.0423299722903536 1107 70-71 1.4899569472523666 1107 72-73 0.9008696601562463 1107 74-75 0.7010990609611376 1107 76-77 0.8798453851756278 1107 78-79 0.8479999995700425 1107 80-81 0.9282403339927434 1107 82-83 1.063957355301394 1107 84-85 1.0440362749629024 1107 86-87 0.9812802845184905 1107 88-89 1.0164312544861538 1107 90-91 1.035412167451188 1107 92-93 0.9481316085981284 1107 94-95 1.0382907669242059 1107 96-97 1.2387830876837072 1107 98-99 1.229611209151276 1107 100-101 1.0115227141373282 1108 1 0.5358072132615845 1108 2 0.40560873590307267 1108 3 0.21041878524884083 1108 4 0.4429952429754067 1108 5 0.44066036334393743 1108 6 0.4707722442821023 1108 7 0.5111733346792704 1108 8 0.4518606943284311 1108 9 0.5788310607840401 1108 10-11 0.5702297732432982 1108 12-13 0.7942665790521346 1108 14-15 0.9641877604483469 1108 16-17 0.6379212984380658 1108 18-19 0.5316299635357637 1108 20-21 0.5045781232411741 1108 22-23 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2.1120171675795376 2311 62-63 2.0295990618877298 2311 64-65 -1.1930982448589802 2311 66-67 1.1453459527281922 2311 68-69 1.5613647435222333 2311 70-71 2.1314197020233365 2311 72-73 1.5860640924473515 2311 74-75 1.5929110921328622 2311 76-77 1.5414744927400754 2311 78-79 1.5055138807970394 2311 80-81 1.4964839756827146 2311 82-83 1.5697296630197144 2311 84-85 1.372190172168903 2311 86-87 1.1138943515330624 2311 88-89 1.2160104847464872 2311 90-91 1.496748168178275 2311 92-93 1.4130924748458717 2311 94-95 1.4228263825489584 2311 96-97 1.6102034008805397 2311 98-99 1.8211694468959543 2311 100-101 1.9113607048416092 2312 1 -0.08748903475019532 2312 2 -0.11024007074275133 2312 3 -0.2938314710575618 2312 4 0.21943741476609802 2312 5 -0.9991057333310422 2312 6 0.05246163045463703 2312 7 0.37139162757314637 2312 8 -2.1871413350276185 2312 9 -4.322132145007853 2312 10-11 -0.3926302686939138 2312 12-13 -5.40032124228015 2312 14-15 -2.0689280816078437 2312 16-17 -3.2497027939419283 2312 18-19 -2.259465340255886 2312 20-21 -3.344139500935583 2312 22-23 -1.8216370432530837 2312 24-25 -3.6291228793237664 2312 26-27 -3.642502569864881 2312 28-29 -5.653959166534644 2312 30-31 -6.46761909897387 2312 32-33 -1.5050584290034266 2312 34-35 0.7161492068681383 2312 36-37 1.1936409258060365 2312 38-39 1.1503555436453823 2312 40-41 -2.9678716273169954 2312 42-43 -4.445487934644678 2312 44-45 -3.7729381177441645 2312 46-47 0.12791590556532384 2312 48-49 -0.9963339998828644 2312 50-51 -5.997897740210735 2312 52-53 -1.0277557791837637 2312 54-55 0.9031421071374766 2312 56-57 1.3369308644449163 2312 58-59 1.3855542026327257 2312 60-61 1.6470316146507216 2312 62-63 1.3949727886483672 2312 64-65 1.6038284690890094 2312 66-67 1.2764071557752104 2312 68-69 1.441958970530962 2312 70-71 1.9796568674787807 2312 72-73 1.5920997577422824 2312 74-75 1.2193142091979219 2312 76-77 1.2310040143814973 2312 78-79 1.0807189124638512 2312 80-81 1.1811832139280547 2312 82-83 0.0616816228597763 2312 84-85 0.4248971738908729 2312 86-87 1.2467738425291799 2312 88-89 1.3099529299497696 2312 90-91 1.2780997719491047 2312 92-93 1.3842231405785874 2312 94-95 1.4440227167870603 2312 96-97 1.7203586704132725 2312 98-99 1.7589157037911463 2312 100-101 1.8881359434371774 2313 1 -0.14684616126586647 2313 2 -0.08061298311978149 2313 3 -0.3149021642627794 2313 4 -0.917968213236648 2313 5 -2.0630384718348154 2313 6 -1.9974926134254858 2313 7 -2.3190224653117752 2313 8 -3.831524771312221 2313 9 -3.247675498931443 2313 10-11 -1.379921506396954 2313 12-13 -3.255011244567953 2313 14-15 -2.32718706124637 2313 16-17 -5.913326678636508 2313 18-19 -3.808807596032594 2313 20-21 -4.357260068311476 2313 22-23 -3.8932968465017694 2313 24-25 -1.165270214029782 2313 26-27 -3.7104160907983044 2313 28-29 -4.0103421452580506 2313 30-31 -2.8244138827762058 2313 32-33 -6.370409607223515 2313 34-35 -2.9990418249323625 2313 36-37 -4.427755779753333 2313 38-39 -2.632222813709916 2313 40-41 -1.1380100395796404 2313 42-43 -2.434500746358239 2313 44-45 -3.94881320353689 2313 46-47 -2.3542392076810827 2313 48-49 -0.7146192892903187 2313 50-51 1.049030880242256 2313 52-53 0.3074592745796778 2313 54-55 -4.209435106314828 2313 56-57 -5.204421228897566 2313 58-59 -0.6909271517484257 2313 60-61 -6.442741892555869 2313 62-63 -1.3484154520288172 2313 64-65 -2.497309714393033 2313 66-67 -3.229106456670454 2313 68-69 -4.600445513568786 2313 70-71 -4.507657248284197 2313 72-73 -0.2404271239781508 2313 74-75 1.4319028616801965 2313 76-77 -1.2984686463368753 2313 78-79 0.6790945702080791 2313 80-81 1.4671132070646316 2313 82-83 1.987928477126534 2313 84-85 1.9052392008869852 2313 86-87 2.1356265078231615 2313 88-89 -2.6204977678310613 2313 90-91 1.141655022463862 2313 92-93 2.0087770184097486 2313 94-95 2.2817028815090943 2313 96-97 2.447309025023202 2313 98-99 2.6498731505996567 2313 100-101 2.5697305442150338 2314 1 -28.783942935459415 2314 2 -14.819262923382627 2314 3 -5.3515409535897405 2314 4 -3.7969009093736474 2314 5 -1.612620311739235 2314 6 -1.0814233183239281 2314 7 -0.3515792156104567 2314 8 -3.6934323778434823 2314 9 -3.0361064029537417 2314 10-11 -0.479483433915874 2314 12-13 0.46825637789321206 2314 14-15 -1.131398530780423 2314 16-17 -0.931446949444954 2314 18-19 -3.176191108577399 2314 20-21 -2.435963772015178 2314 22-23 -5.475485217668634 2314 24-25 -1.0776059368493733 2314 26-27 -4.508952530463773 2314 28-29 -3.2233051082210125 2314 30-31 -4.4070005016531475 2314 32-33 -2.2703000891032445 2314 34-35 -2.279109527043083 2314 36-37 -5.928502494209727 2314 38-39 -7.8673880865095995 2314 40-41 -6.480801756027269 2314 42-43 -4.351864346517541 2314 44-45 -4.890320571119524 2314 46-47 -6.0578931304210215 2314 48-49 -5.137160109680604 2314 50-51 -1.9425063559186384 2314 52-53 -3.0203483717763575 2314 54-55 -3.735819017107339 2314 56-57 -1.6136207510003118 2314 58-59 -1.616803296710593 2314 60-61 -0.022242091679729015 2314 62-63 0.7967458382937664 2314 64-65 -0.5958760226367623 2314 66-67 0.5650990391479453 2314 68-69 0.876008090572995 2314 70-71 0.9903017322016652 2314 72-73 0.6464207453965969 2314 74-75 0.52758187402587 2314 76-77 -0.7292731067112221 2314 78-79 -1.9802542947859507 2314 80-81 -1.559071579872839 2314 82-83 -1.6826708856771297 2314 84-85 -0.30339281822890385 2314 86-87 0.4794636245097408 2314 88-89 0.26162090998654364 2314 90-91 0.893696041978 2314 92-93 1.0115149714165206 2314 94-95 1.3468661630702208 2314 96-97 1.2389756916898698 2314 98-99 1.1591324098589162 2314 100-101 1.2128010340597157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 20.0 4 52.0 5 93.0 6 191.0 7 609.0 8 1469.0 9 2731.0 10 4393.0 11 6755.0 12 9055.0 13 11408.0 14 14068.0 15 16806.0 16 19259.0 17 21539.0 18 19133.0 19 15495.0 20 14458.0 21 15025.0 22 16040.0 23 18356.0 24 22204.0 25 27327.0 26 34595.0 27 44083.0 28 55491.0 29 69877.0 30 86636.0 31 105896.0 32 128520.0 33 156373.0 34 191915.0 35 235019.0 36 294885.0 37 372926.0 38 359233.0 39 89347.0 40 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.3142202788914 21.547306495821555 15.353888539557808 22.78458468572924 2 14.669732641957516 21.949973180776077 41.134362498382984 22.24593167888342 3 17.97454580626441 26.74556284558219 31.475827382199796 23.804063965953606 4 12.082244909966802 17.941878393724878 39.9001296839146 30.075747012393723 5 11.552386578964125 38.890424749074924 36.69357601178036 12.86361266018059 6 28.9533168186557 39.879440435329805 18.1602213885628 13.007021357451695 7 25.05878669105584 33.8554921377964 23.402402406032977 17.683318765114777 8 23.970824875505205 37.095117369870344 20.900900247397768 18.033157507226683 9 24.54565494317992 16.514919427475057 21.902661732413055 37.036763896931966 10-11 23.06088548171235 27.823443668950432 29.623535470891976 19.492135378445244 12-13 24.0373389687958 25.320611631443015 29.680841160476483 20.961208239284705 14-15 21.14253520854314 27.05435053753301 27.730835740212594 24.072278513711254 16-17 20.30868100598486 30.005734598894428 28.45686900572211 21.22871538939861 18-19 20.076556693743807 28.710291529598468 30.920711412657788 20.292440363999937 20-21 21.084846675040875 27.84401649210999 30.999657858435604 20.071478974413534 22-23 20.686447204413263 27.81012473054841 30.709945761091696 20.793482303946636 24-25 20.50553498246775 27.920754803682758 30.986106817120053 20.58760339672944 26-27 20.62019934028807 28.079248454751056 30.672917049960414 20.627635155000466 28-29 20.49963758310551 28.119218533659428 30.813516608608094 20.567627274626968 30-31 20.870266093192562 27.906809450837088 30.735572219753827 20.487352236216523 32-33 20.711063449767792 28.101238173995608 30.499968566081353 20.68772981015525 34-35 20.86583511278288 28.11254893276351 30.546517534790198 20.47509841966342 36-37 20.85204088829066 27.914278057302443 30.54443166041682 20.689249393990075 38-39 20.687009980232972 28.02803250577798 30.57014070657551 20.71481680741354 40-41 20.95986971702706 27.989359095466693 30.455095737512533 20.595675449993713 42-43 20.87486081384257 28.00115177426836 30.328900466633808 20.79508694525526 44-45 20.88375910243021 28.232358881225462 30.119853156157294 20.764028860187032 46-47 20.959853122951476 28.221724192704368 29.78686863067147 21.03155405367269 48-49 21.018236402104545 28.200459749358807 29.77449467273593 21.006809175800722 50-51 21.029054938105293 28.368836947420554 29.545889039643907 21.05621907483024 52-53 21.00616450459995 28.508954782684743 29.456430253525905 21.0284504591894 54-55 21.074166436879473 28.366200964622447 29.476637495787234 21.082995102710843 56-57 21.106857931463605 28.51888934987204 29.247043466994494 21.127209251669864 58-59 20.991555718815732 28.461320066003502 29.41155609360665 21.135568121574114 60-61 21.066611696445896 28.508809844344405 29.35633458245503 21.068243876754668 62-63 21.06548220199843 28.50243375974169 29.198729238082876 21.233354800177 64-65 20.892271952532322 28.41569928538121 29.297214272385393 21.394814489701083 66-67 20.962731542493554 28.455011780143746 29.147573806247866 21.43468287111483 68-69 21.1242049003948 28.38317438561699 29.123007175668782 21.369613538319435 70-71 21.10729969529944 28.082546628495635 29.118419724268257 21.691733951936666 72-73 21.062717513763403 28.32215341220167 29.114799711523176 21.500329362511756 74-75 21.064560328846618 28.40428790199081 28.972031917465944 21.55911985169662 76-77 20.873657880197257 28.586612794482534 28.773480925708533 21.766248399611676 78-79 20.961026892165766 28.617542553101462 28.674304201329154 21.747126353403623 80-81 20.911599187481198 28.561212644617324 28.697586200156806 21.829601967744672 82-83 21.06282101139874 28.478710735608516 28.774368656593115 21.684099596399633 84-85 21.18087270331291 28.484934578987296 28.464019198626595 21.870173519073195 86-87 20.794633663725445 28.676893440987833 28.60612679561295 21.922346099673774 88-89 21.046622502250806 28.49842060762477 28.53652511108944 21.918431779034986 90-91 20.979694099734946 28.480549464676553 28.564473963658376 21.97528247193013 92-93 20.97603802449314 28.477706472449654 28.531628775971768 22.01462672708544 94-95 20.822919227374758 28.517012504987083 28.510786206229522 22.149282061408634 96-97 20.75904119415496 28.501160625770726 28.530800912380816 22.208997267693498 98-99 20.704026320789865 28.517970512026654 28.447304317408285 22.330698849775196 100-101 20.751764009155497 28.492044956970002 28.32687280575638 22.429318228118113 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 436.0 1 310.5 2 227.0 3 291.5 4 254.0 5 231.5 6 397.0 7 832.5 8 1383.0 9 1799.0 10 1854.5 11 1779.5 12 1995.5 13 2183.5 14 2373.5 15 2708.0 16 3077.0 17 3384.0 18 3747.0 19 4297.0 20 4931.5 21 5967.5 22 7359.5 23 9314.5 24 11873.0 25 15135.5 26 19753.0 27 25403.0 28 31627.5 29 38122.5 30 44084.5 31 50237.5 32 57273.5 33 63727.5 34 70077.5 35 75833.5 36 82026.5 37 88111.5 38 90353.0 39 90894.0 40 91770.0 41 92167.0 42 92951.0 43 93949.0 44 94235.0 45 93883.5 46 92170.0 47 90162.0 48 88332.0 49 85336.0 50 80752.0 51 75400.5 52 70181.0 53 64380.0 54 58596.5 55 52835.5 56 45901.0 57 38662.0 58 33084.0 59 28844.5 60 23886.5 61 18654.0 62 14383.5 63 10876.0 64 8070.0 65 6110.0 66 4643.5 67 3555.0 68 2711.0 69 2040.0 70 1579.0 71 1228.0 72 964.5 73 817.5 74 646.5 75 519.0 76 424.5 77 314.0 78 233.5 79 177.0 80 119.0 81 96.0 82 88.0 83 64.5 84 49.5 85 36.0 86 25.5 87 23.0 88 19.5 89 12.0 90 5.0 91 5.0 92 5.0 93 1.5 94 1.5 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21229702723712826 2 0.0 3 0.004392630214283786 4 4.432929574047857E-4 5 4.432929574047857E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.023051233785048857 26-27 0.007838225474111893 28-29 0.006871040839774178 30-31 0.002478410625490393 32-33 0.0013298788722143572 34-35 0.0036470920586484643 36-37 0.0013701782319784285 38-39 9.671846343377144E-4 40-41 0.002458260945608357 42-43 0.0016119743905628572 44-45 7.052387958712499E-4 46-47 0.0397754680871385 48-49 0.02063327219920457 50-51 0.08289578303469493 52-53 0.00183362086926525 54-55 0.03518134107403436 56-57 7.052387958712499E-4 58-59 0.00183362086926525 60-61 0.0034455952598281075 62-63 0.0033448468604179286 64-65 0.009913642501961572 66-67 0.005440413568149643 68-69 0.002317213186434107 70-71 0.005662060046852035 72-73 0.004856072851570607 74-75 0.0011686814331580715 76-77 0.0 78-79 0.0 80-81 3.8284391775867863E-4 82-83 5.64191036697E-4 84-85 6.044903964610715E-5 86-87 0.0013701782319784285 88-89 0.003828439177586786 90-91 0.0012895795124502857 92-93 0.0032642481408897855 94-95 7.656878355173573E-4 96-97 3.6269423787664286E-4 98-99 0.0017933215095011785 100-101 0.003163499741479607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2481429.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.1048034221164 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49879316622372 56.18564091147506 2 11.313080228008014 15.409502100542353 3 2.9984805282737783 6.126327808293882 4 1.1179734041313778 3.045574356780871 5 0.5776122663569497 1.96690849272216 6 0.3286459900278425 1.3429422327787826 7 0.211422612696154 1.0079226833663273 8 0.15132858246660802 0.8244962688828693 9 0.11152643518658668 0.6835937350276704 >10 0.6272002847647032 7.869749814590414 >50 0.04443693688266422 2.0557250760435677 >100 0.018544913663440965 2.232327539640448 >500 6.563227811635497E-4 0.27363752245914624 >1k 1.7899712213113167E-4 0.31736101133969286 >5k 1.1933141475408778E-4 0.6582904460567253 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8793 0.35435227040548006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7407 0.29849735777247705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4750 0.1914219588793393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.029935976407143E-5 0.0 9 0.0 0.0 0.0 4.029935976407143E-5 0.0 10-11 4.029935976407143E-5 0.0 0.0 4.029935976407143E-5 0.0 12-13 8.059871952814285E-5 0.0 0.0 4.029935976407143E-5 4.029935976407143E-5 14-15 1.0074839941017857E-4 0.0 0.0 4.029935976407143E-5 1.208980792922143E-4 16-17 1.2089807929221428E-4 0.0 0.0 4.029935976407143E-5 2.0149679882035715E-4 18-19 1.2089807929221428E-4 0.0 0.0 4.029935976407143E-5 2.0149679882035715E-4 20-21 1.2089807929221428E-4 0.0 0.0 4.029935976407143E-5 2.4179615858442856E-4 22-23 1.4104775917425E-4 0.0 0.0 1.8134711893832143E-4 2.4179615858442856E-4 24-25 1.611974390562857E-4 0.0 0.0 4.029935976407143E-4 2.4179615858442856E-4 26-27 1.611974390562857E-4 0.0 0.0 9.268852745736428E-4 2.4179615858442856E-4 28-29 1.611974390562857E-4 0.0 0.0 0.0024985603053724287 2.820955183485E-4 30-31 1.611974390562857E-4 0.0 0.0 0.008200919711988535 2.820955183485E-4 32-33 1.611974390562857E-4 0.0 0.0 0.023696023541274 2.820955183485E-4 34-35 2.0149679882035715E-4 0.0 0.0 0.05049509778438149 3.223948781125714E-4 36-37 2.4179615858442856E-4 0.0 0.0 0.08996832067328947 3.223948781125714E-4 38-39 2.4179615858442856E-4 0.0 0.0 0.1458433829861745 3.223948781125714E-4 40-41 2.820955183485E-4 0.0 0.0 0.20312892289080203 3.223948781125714E-4 42-43 2.820955183485E-4 0.0 0.0 0.2605555105546038 3.223948781125714E-4 44-45 3.022451982305357E-4 0.0 0.0 0.3348272305997875 3.828439177586785E-4 46-47 3.223948781125714E-4 0.0 0.0 0.4240500131174416 4.432929574047857E-4 48-49 3.223948781125714E-4 0.0 0.0 0.5158922540197604 4.432929574047857E-4 50-51 3.223948781125714E-4 0.0 0.0 0.611018892742851 5.238916769329286E-4 52-53 3.223948781125714E-4 0.0 0.0 0.7012290095747249 5.64191036697E-4 54-55 3.223948781125714E-4 0.0 0.0 0.7996601957984694 6.044903964610714E-4 56-57 3.223948781125714E-4 0.0 0.0 0.9197321382155201 6.447897562251428E-4 58-59 3.223948781125714E-4 0.0 0.0 1.0485691913812565 6.850891159892143E-4 60-61 3.4254455799460714E-4 0.0 0.0 1.1798846551724833 7.455381556353215E-4 62-63 3.6269423787664286E-4 0.0 0.0 1.3121068545584016 7.656878355173572E-4 64-65 3.6269423787664286E-4 0.0 0.0 1.4523486265373702 8.059871952814286E-4 66-67 3.6269423787664286E-4 0.0 0.0 1.6160647755789104 8.059871952814286E-4 68-69 3.6269423787664286E-4 0.0 0.0 1.795276028449736 8.059871952814286E-4 70-71 4.432929574047857E-4 0.0 0.0 1.9900428341894933 8.462865550454999E-4 72-73 4.432929574047857E-4 0.0 0.0 2.1961337600229545 8.865859148095714E-4 74-75 4.432929574047857E-4 0.0 0.0 2.4011164534629037 9.06735594691607E-4 76-77 4.432929574047857E-4 0.0 0.0 2.6318302881122126 9.268852745736428E-4 78-79 4.835923171688571E-4 0.0 0.0 2.8866834392602003 9.268852745736428E-4 80-81 4.835923171688571E-4 0.0 0.0 3.1606787862961223 9.470349544556786E-4 82-83 4.835923171688571E-4 0.0 0.0 3.4385227221895125 9.671846343377142E-4 84-85 4.835923171688571E-4 0.0 0.0 3.7125583685851984 9.671846343377142E-4 86-87 4.835923171688571E-4 0.0 0.0 4.0123453058701255 0.0010074839941017857 88-89 4.835923171688571E-4 0.0 0.0 4.336231260293967 0.001067933033747893 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4175 0.0 61.784073 1 GTATCAA 8120 0.0 40.324238 1 ATCAACG 9365 0.0 34.28163 3 CAACGCA 9670 0.0 33.197018 5 TCAACGC 9755 0.0 32.907753 4 AACGCAG 10035 0.0 31.894907 6 TATCAAC 10210 0.0 31.770023 2 GTGGTAT 1740 0.0 29.539846 1 ACGCAGA 11410 0.0 27.843208 7 TGGTATC 1810 0.0 27.813128 2 CGCAGAG 11505 0.0 27.159267 8 GCAGAGT 13080 0.0 23.45328 9 GAGTACT 9420 0.0 22.281797 12-13 CAGAGTA 12840 0.0 21.672619 10-11 GTACTTT 10155 0.0 20.715843 14-15 TACATGG 6790 0.0 20.074036 2 GTACATG 7110 0.0 19.947128 1 AGAGTAC 12305 0.0 19.85558 10-11 ACATGGG 6645 0.0 19.797363 3 GTACTAG 755 0.0 18.910719 1 >>END_MODULE