FastQCFastQC Report
Sat 18 Jun 2016
SRR1931110_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931110_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000146
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.19873549230906146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29220.14608933547851005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.047.7140731
GTATCAA42250.042.1019061
TCAACGC49850.034.1977584
ATCAACG49150.034.194723
CAACGCA50200.034.1269875
TATCAAC53000.032.569932
AACGCAG53900.032.5552336
ACGCAGA61900.028.205447
CGCAGAG63650.026.9060968
GTACATG50700.024.1444591
GCAGAGT69500.023.4092719
TACATGG51250.023.2322352
ACATGGG51900.021.8952313
GAGTACT42200.021.24960912-13
GTATAGG5200.021.1499821
CTATACG2108.186962E-720.4652864
GTGGTAT12650.020.0341471
AGAGTAC64600.019.91963410-11
CAGAGTA70650.018.81985510-11
TCTAACG2051.5016833E-518.6604292
Produced by FastQC (version 0.11.5)