##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931110_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2000146 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.301529488347352 31.0 30.0 33.0 25.0 34.0 2 29.061049543383334 31.0 28.0 34.0 23.0 34.0 3 29.106277741724853 31.0 28.0 34.0 23.0 34.0 4 32.326100194685786 35.0 32.0 37.0 22.0 37.0 5 32.44032035661397 35.0 32.0 37.0 24.0 37.0 6 32.7607429657635 35.0 32.0 37.0 26.0 37.0 7 32.48858783308818 35.0 32.0 37.0 24.0 37.0 8 32.627146718289566 35.0 32.0 37.0 25.0 37.0 9 33.814602534015016 37.0 33.0 39.0 25.0 39.0 10-11 33.70316466897916 37.0 32.5 39.0 24.0 39.0 12-13 33.76915810145859 37.0 33.0 39.0 25.0 39.0 14-15 34.572288722923226 38.0 33.0 40.0 24.0 41.0 16-17 34.553512093617165 38.0 33.0 40.0 24.0 41.0 18-19 34.56021535427914 38.0 33.0 40.0 24.0 41.0 20-21 34.722420513302524 38.0 33.0 40.0 25.0 41.0 22-23 34.60230353184218 38.0 33.0 40.0 25.0 41.0 24-25 34.51632705812476 38.0 33.0 40.0 24.5 41.0 26-27 34.1667668260217 38.0 32.5 40.0 22.0 41.0 28-29 34.12305776678302 38.0 32.0 40.0 23.0 41.0 30-31 33.92595515527366 38.0 32.0 40.0 22.0 41.0 32-33 33.690858817306335 38.0 32.0 40.0 21.0 41.0 34-35 33.65017628713104 38.0 32.0 40.0 22.0 41.0 36-37 33.3494377410449 37.5 31.5 40.0 19.0 41.0 38-39 33.14541338482291 37.0 31.0 40.0 18.0 41.0 40-41 33.02502892288863 37.5 31.0 40.0 16.0 41.0 42-43 33.27328105048331 38.0 31.5 40.0 17.0 41.0 44-45 33.304795749910255 38.0 32.0 40.0 18.0 41.0 46-47 33.07851826816642 38.0 31.5 40.0 15.5 41.0 48-49 32.84798959675943 37.0 31.0 40.0 14.5 41.0 50-51 32.31203497144708 36.5 30.5 39.5 11.0 40.5 52-53 32.26316653884267 37.0 30.0 39.5 10.5 40.5 54-55 32.24061918479951 37.0 30.0 40.0 8.5 41.0 56-57 32.018085929727135 36.5 30.0 40.0 8.0 41.0 58-59 31.83292794625992 36.0 30.0 40.0 7.0 41.0 60-61 31.63201111318874 36.0 30.0 40.0 7.0 41.0 62-63 31.466956912145413 35.5 30.0 39.0 4.0 41.0 64-65 31.148640899214357 35.0 29.0 39.0 2.0 41.0 66-67 30.897281248468865 35.0 29.0 39.0 2.0 40.0 68-69 30.688759720540403 35.0 29.0 39.0 2.0 40.0 70-71 30.356103004480673 35.0 29.0 38.0 2.0 40.0 72-73 30.00416819572171 34.0 29.0 37.5 2.0 40.0 74-75 29.59619022811335 34.0 29.0 37.0 2.0 39.5 76-77 29.263007050485314 34.0 29.0 36.0 2.0 39.0 78-79 28.846978170593545 34.0 28.0 36.0 2.0 39.0 80-81 28.463426169889598 34.0 28.0 35.5 2.0 37.5 82-83 28.12596180478825 33.5 27.0 35.0 2.0 37.0 84-85 27.778041452973934 33.0 27.0 35.0 2.0 37.0 86-87 27.456489426271883 33.0 26.5 35.0 2.0 36.0 88-89 27.125617829898417 33.0 26.0 35.0 2.0 36.0 90-91 26.759728539816592 33.0 25.5 35.0 2.0 35.5 92-93 26.403566789624357 33.0 25.0 35.0 2.0 35.0 94-95 26.100277179765875 33.0 24.5 35.0 2.0 35.0 96-97 25.733746936473636 32.0 23.5 35.0 2.0 35.0 98-99 25.35213929383155 32.0 21.5 35.0 2.0 35.0 100-101 24.757903423050116 32.0 18.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.176123575712015 1101 2 5.824244174088882 1101 3 5.389161741850227 1101 4 6.812643837447393 1101 5 6.158373936371721 1101 6 5.745068376867387 1101 7 6.382024502063899 1101 8 5.853645144314836 1101 9 6.471968342533621 1101 10-11 6.529743323267962 1101 12-13 6.431687686117655 1101 14-15 6.4925675914200625 1101 16-17 6.552941390863097 1101 18-19 6.651377600555033 1101 20-21 5.823399034970624 1101 22-23 5.987093241930001 1101 24-25 5.9419784359442644 1101 26-27 6.045383727874963 1101 28-29 5.863928530582534 1101 30-31 6.064657506199662 1101 32-33 5.803484804845578 1101 34-35 5.664429925861228 1101 36-37 5.670249217036574 1101 38-39 5.778465336529621 1101 40-41 6.002747045837118 1101 42-43 5.797720105394344 1101 44-45 5.720228760419499 1101 46-47 5.556480010264266 1101 48-49 5.472572477902386 1101 50-51 5.4804768429980975 1101 52-53 5.212118463922206 1101 54-55 5.5106056374920875 1101 56-57 5.461256640274229 1101 58-59 5.260221493266982 1101 60-61 5.163590890704555 1101 62-63 5.181544475031821 1101 64-65 5.1878342096758345 1101 66-67 5.100628744219943 1101 68-69 5.117179893842781 1101 70-71 4.8759946833784475 1101 72-73 4.601548440921231 1101 74-75 4.789738578535314 1101 76-77 4.666648270751907 1101 78-79 4.549573177015841 1101 80-81 4.5066673963026 1101 82-83 4.500871588040965 1101 84-85 4.422906171571892 1101 86-87 4.190309298757349 1101 88-89 4.383065939851388 1101 90-91 4.260673932193637 1101 92-93 4.210723755128843 1101 94-95 4.179923677286325 1101 96-97 3.924437133397415 1101 98-99 3.666162706643714 1101 100-101 3.5217800742940533 1102 1 5.164432005485313 1102 2 5.732673168988448 1102 3 5.508262303045598 1102 4 6.925215536709839 1102 5 6.3568470023577 1102 6 5.8856890027363065 1102 7 6.366233011428136 1102 8 5.9459187903722714 1102 9 6.778320147285335 1102 10-11 6.7595007654936445 1102 12-13 6.760386844032702 1102 14-15 6.8874857945607175 1102 16-17 6.87674374595435 1102 18-19 6.900193605367743 1102 20-21 6.239805958465187 1102 22-23 6.342489037982592 1102 24-25 6.081349459003391 1102 26-27 6.22027405055605 1102 28-29 6.147039842810045 1102 30-31 6.56321994172141 1102 32-33 6.1659224046296295 1102 34-35 6.11564641906152 1102 36-37 6.296418547808241 1102 38-39 6.285382318979302 1102 40-41 6.320557770904202 1102 42-43 6.216314285553427 1102 44-45 6.198849362936553 1102 46-47 6.106934148451153 1102 48-49 5.8631915747801 1102 50-51 5.853803396877108 1102 52-53 5.618747245430203 1102 54-55 5.88401197691201 1102 56-57 5.869374937211546 1102 58-59 5.87556817256219 1102 60-61 5.624725880333827 1102 62-63 5.59896404244963 1102 64-65 5.611503111634974 1102 66-67 5.4314402729804065 1102 68-69 5.597004159933956 1102 70-71 5.371742906343176 1102 72-73 5.075875844641349 1102 74-75 5.261311226606296 1102 76-77 5.3463127642124135 1102 78-79 5.087205194981205 1102 80-81 5.158834025485817 1102 82-83 5.038841860560012 1102 84-85 4.930947657551183 1102 86-87 4.962364579851261 1102 88-89 4.944053124681126 1102 90-91 4.920443887127316 1102 92-93 4.962321689934324 1102 94-95 4.957393672223972 1102 96-97 4.76888879267257 1102 98-99 4.516159426262593 1102 100-101 4.428262142146238 1103 1 4.930230512699307 1103 2 5.582417225610477 1103 3 5.1861372098969944 1103 4 6.644702350564135 1103 5 6.063194157296206 1103 6 5.417175120969013 1103 7 6.195202024062738 1103 8 5.545516175574409 1103 9 6.140051144480168 1103 10-11 6.270201583241072 1103 12-13 6.376187570570806 1103 14-15 6.590767411808095 1103 16-17 6.613079955494392 1103 18-19 6.35686070703127 1103 20-21 5.6052203335805295 1103 22-23 5.995774463786173 1103 24-25 5.730232081821633 1103 26-27 5.795519079340053 1103 28-29 5.714793532558893 1103 30-31 6.065302360837901 1103 32-33 5.687831019163692 1103 34-35 5.6822656981410375 1103 36-37 5.705703884838858 1103 38-39 5.618709153030153 1103 40-41 5.626964763581412 1103 42-43 5.6129021482448955 1103 44-45 5.3813005581091 1103 46-47 5.359649144949238 1103 48-49 5.28537472040669 1103 50-51 5.208569846969478 1103 52-53 4.990082630249805 1103 54-55 5.449085500249357 1103 56-57 5.452052706472969 1103 58-59 5.596692385542248 1103 60-61 5.191532996845112 1103 62-63 5.294475749979405 1103 64-65 5.209676733910769 1103 66-67 5.317350106252459 1103 68-69 5.243262799545686 1103 70-71 5.068075448747667 1103 72-73 4.832501682278679 1103 74-75 4.964229770830496 1103 76-77 4.901798142297025 1103 78-79 4.90322917712356 1103 80-81 4.837930785776688 1103 82-83 4.918423014591749 1103 84-85 4.7803846843345745 1103 86-87 4.747081249873752 1103 88-89 4.78319955862634 1103 90-91 4.639511934825002 1103 92-93 4.549933254727247 1103 94-95 4.495201923741025 1103 96-97 4.431017664973076 1103 98-99 4.322847825036352 1103 100-101 4.168470407161259 1104 1 4.610520536977184 1104 2 5.398089818709298 1104 3 5.040760195920921 1104 4 6.509575973533263 1104 5 5.941902895505091 1104 6 5.659162231384222 1104 7 6.202173406095042 1104 8 5.681582979911116 1104 9 6.258209763402981 1104 10-11 6.294659210049968 1104 12-13 6.396730186851016 1104 14-15 6.190494600308362 1104 16-17 6.3080417698149915 1104 18-19 6.484297330595876 1104 20-21 5.855665910755118 1104 22-23 5.922449546048565 1104 24-25 5.772607100398119 1104 26-27 5.870726679167788 1104 28-29 5.843823234600599 1104 30-31 6.085180843865285 1104 32-33 5.942524380348257 1104 34-35 5.680621519228836 1104 36-37 5.770211701791727 1104 38-39 5.708113846753378 1104 40-41 5.972001659681673 1104 42-43 6.00538293644669 1104 44-45 5.955029934756201 1104 46-47 5.84200504741154 1104 48-49 5.7291055900692065 1104 50-51 5.785563410452458 1104 52-53 5.457781396977698 1104 54-55 5.703834375401893 1104 56-57 5.635448582095478 1104 58-59 5.468677278846819 1104 60-61 5.305890219229589 1104 62-63 5.2849512426313865 1104 64-65 5.376000374827115 1104 66-67 5.354096491206999 1104 68-69 5.395357727741299 1104 70-71 5.255389024395448 1104 72-73 5.162403850116366 1104 74-75 5.2992891862925475 1104 76-77 5.283433604481843 1104 78-79 5.192999332055802 1104 80-81 5.102219512200062 1104 82-83 4.81499594080886 1104 84-85 4.780010740867102 1104 86-87 4.7687039748785836 1104 88-89 4.841353095090227 1104 90-91 4.760491421896624 1104 92-93 4.759585513163042 1104 94-95 4.559441825457164 1104 96-97 4.323805124747004 1104 98-99 4.270849373910536 1104 100-101 4.366835951813627 1105 1 4.655513660571021 1105 2 5.30131232784322 1105 3 5.051190578047951 1105 4 6.568350333393415 1105 5 3.778935677049649 1105 6 5.12617843958737 1105 7 5.972603219743341 1105 8 1.027699067640505 1105 9 1.7208274767628886 1105 10-11 2.6795683955730034 1105 12-13 5.866146632503359 1105 14-15 4.468711588639245 1105 16-17 6.065894210228585 1105 18-19 6.599038202685971 1105 20-21 4.262541988951551 1105 22-23 5.823017532824881 1105 24-25 5.967363430736739 1105 26-27 6.060254153088827 1105 28-29 3.9636100113480737 1105 30-31 5.985861323836243 1105 32-33 6.020040718645578 1105 34-35 6.0441393967916355 1105 36-37 6.001170233454889 1105 38-39 6.011221457590338 1105 40-41 6.208676026319843 1105 42-43 4.005503683515197 1105 44-45 5.780716208837287 1105 46-47 5.967971515912353 1105 48-49 5.72416523660662 1105 50-51 5.895961433021061 1105 52-53 5.92924562326721 1105 54-55 6.202066242570279 1105 56-57 5.977675182089783 1105 58-59 6.007420634751675 1105 60-61 5.795965072573011 1105 62-63 5.684365108264878 1105 64-65 5.847992411751996 1105 66-67 5.863851258229108 1105 68-69 5.894623788905243 1105 70-71 5.684652997493284 1105 72-73 5.493226329514481 1105 74-75 5.679413748727914 1105 76-77 5.464642540092882 1105 78-79 5.483903289760985 1105 80-81 5.492178975293768 1105 82-83 5.370530463866139 1105 84-85 5.2519281649587946 1105 86-87 5.312748012301977 1105 88-89 5.28706867862271 1105 90-91 5.323026651179401 1105 92-93 5.261016767686275 1105 94-95 5.149215111767106 1105 96-97 5.214690169602161 1105 98-99 5.228718048764755 1105 100-101 5.259202703679222 1106 1 4.136379374865129 1106 2 4.420701094260579 1106 3 4.5645024606372 1106 4 6.130067659529278 1106 5 5.950972619598804 1106 6 5.184895077249344 1106 7 5.898374618990623 1106 8 5.543417915666016 1106 9 6.08115052085558 1106 10-11 5.950052697291053 1106 12-13 6.2546210850295125 1106 14-15 5.891007076148291 1106 16-17 5.971117406020568 1106 18-19 6.1483652023206865 1106 20-21 5.416013885408216 1106 22-23 5.530964680946656 1106 24-25 5.62187709689448 1106 26-27 5.781864520937589 1106 28-29 5.6665132068266715 1106 30-31 5.935487780232737 1106 32-33 5.791186072817904 1106 34-35 5.2601659524928905 1106 36-37 5.378122786537407 1106 38-39 5.525166236695981 1106 40-41 5.649546965376679 1106 42-43 2.3389577609357026 1106 44-45 4.693942056673787 1106 46-47 4.951880764672758 1106 48-49 4.838127864128534 1106 50-51 5.087821869668243 1106 52-53 4.75451476954979 1106 54-55 5.141524243214679 1106 56-57 5.048933208980422 1106 58-59 5.06281168890019 1106 60-61 4.79482279841546 1106 62-63 4.73002951005158 1106 64-65 4.87741169430932 1106 66-67 4.877908171417744 1106 68-69 4.925053869631984 1106 70-71 4.808399568589724 1106 72-73 4.670946364496395 1106 74-75 4.775694569686507 1106 76-77 4.624295276996943 1106 78-79 4.722861143015177 1106 80-81 4.627364936694178 1106 82-83 4.761515691547292 1106 84-85 4.632006271978781 1106 86-87 4.702562687470596 1106 88-89 4.786154589978999 1106 90-91 4.812605404072119 1106 92-93 4.714794266244201 1106 94-95 4.711567293093303 1106 96-97 4.876904729600749 1106 98-99 4.609989967550295 1106 100-101 4.632346737705305 1107 1 3.1113045278445526 1107 2 -0.07605958350285391 1107 3 1.2258076461279863 1107 4 2.8772652601768485 1107 5 2.8713341153763885 1107 6 4.16540564848669 1107 7 5.113606561790164 1107 8 4.524188530023526 1107 9 5.039322737630918 1107 10-11 2.6899628516629512 1107 12-13 4.992294921665085 1107 14-15 3.520654036086249 1107 16-17 1.977276870461825 1107 18-19 2.6427414877686495 1107 20-21 2.4081823928191106 1107 22-23 4.38164942134026 1107 24-25 4.398905320400409 1107 26-27 3.4503738566050686 1107 28-29 4.321024019124913 1107 30-31 3.718165005791672 1107 32-33 4.15366573994147 1107 34-35 4.419581544407578 1107 36-37 4.6899826510388785 1107 38-39 4.731449059009407 1107 40-41 3.387333965238369 1107 42-43 3.894190840013504 1107 44-45 4.475286677391804 1107 46-47 4.5404902919411825 1107 48-49 2.389882219353016 1107 50-51 3.8972671428701595 1107 52-53 3.993505348263213 1107 54-55 4.653734057526112 1107 56-57 3.3780911179417004 1107 58-59 2.567720450800252 1107 60-61 3.405786078309198 1107 62-63 4.2219485937652905 1107 64-65 4.438902202005348 1107 66-67 4.561378946632967 1107 68-69 4.527325019075672 1107 70-71 4.281177208757477 1107 72-73 4.15980068273873 1107 74-75 4.341790654756579 1107 76-77 4.2285126348154805 1107 78-79 4.04503120617154 1107 80-81 4.071884690546575 1107 82-83 4.069318737357094 1107 84-85 3.9375767832216972 1107 86-87 3.934985551300656 1107 88-89 4.046249081604657 1107 90-91 3.933464393267652 1107 92-93 4.050804424253254 1107 94-95 4.079469303960689 1107 96-97 4.007676618289018 1107 98-99 4.11810825093119 1107 100-101 4.173413307416638 1108 1 -1.657264007785681 1108 2 -0.9749013474542458 1108 3 -1.729069201448283 1108 4 -3.6828285177803544 1108 5 -8.800380219416425 1108 6 -7.049054411926843 1108 7 -5.4414025838316675 1108 8 -5.864297577836101 1108 9 -7.6235757540602265 1108 10-11 -3.1123096740966325 1108 12-13 -1.4449971093587592 1108 14-15 -6.379948963716362 1108 16-17 -5.210795818085238 1108 18-19 -3.96535768454714 1108 20-21 -4.633763891178599 1108 22-23 -3.5432855711787177 1108 24-25 -8.094475790985314 1108 26-27 -9.203445452193211 1108 28-29 -6.056284522579048 1108 30-31 -6.024364781200262 1108 32-33 -2.32579001160493 1108 34-35 -0.5868701596787034 1108 36-37 -4.36980393742795 1108 38-39 -5.324571770443285 1108 40-41 -2.992539119478046 1108 42-43 -3.9325368924931823 1108 44-45 -1.3875531547568691 1108 46-47 -2.0573279982877786 1108 48-49 -0.9206827314737147 1108 50-51 -0.1852678964218306 1108 52-53 -1.9526631664931173 1108 54-55 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2313 54-55 -11.404884129997178 2313 56-57 -9.17033899717665 2313 58-59 -7.499379625683833 2313 60-61 -6.0727591551633715 2313 62-63 -9.929891927110944 2313 64-65 -9.854188735611096 2313 66-67 -9.442504091550244 2313 68-69 -10.815343294940527 2313 70-71 -8.966327252814171 2313 72-73 -8.318862218557573 2313 74-75 -8.396094462224845 2313 76-77 -7.81796615555929 2313 78-79 -9.649199148204724 2313 80-81 -10.78328582974132 2313 82-83 -8.339400448381568 2313 84-85 -6.71073370294253 2313 86-87 -6.247938886345203 2313 88-89 -6.943660902510189 2313 90-91 -6.922225826575064 2313 92-93 -6.513236288813761 2313 94-95 -6.508095168201065 2313 96-97 -5.741406553251057 2313 98-99 -6.795670651228011 2313 100-101 -6.331244377272817 2314 1 -10.805654001169216 2314 2 -14.601716937107353 2314 3 -12.826488779884906 2314 4 -25.329320215691443 2314 5 -18.70420516664224 2314 6 -17.038082106561205 2314 7 -15.696404316715503 2314 8 -11.390574398151802 2314 9 -13.488284787111047 2314 10-11 -12.690990476894452 2314 12-13 -14.700274528307055 2314 14-15 -15.326910903285977 2314 16-17 -15.348733371253264 2314 18-19 -15.775374540780795 2314 20-21 -14.831761622676128 2314 22-23 -15.447634321927008 2314 24-25 -17.823964747789248 2314 26-27 -14.687849497689289 2314 28-29 -16.452228786733556 2314 30-31 -17.967439547260945 2314 32-33 -13.006679453616336 2314 34-35 -14.86083515542526 2314 36-37 -14.597611209238655 2314 38-39 -13.554257645864691 2314 40-41 -17.710894297650007 2314 42-43 -18.015723193258804 2314 44-45 -14.652373249907942 2314 46-47 -12.816248411659341 2314 48-49 -12.685030221942855 2314 50-51 -11.882887544173808 2314 52-53 -12.942218603022308 2314 54-55 -13.937492825649354 2314 56-57 -12.701988613544938 2314 58-59 -12.193433334123732 2314 60-61 -12.717260433935751 2314 62-63 -15.515889369566185 2314 64-65 -13.458408684460199 2314 66-67 -15.250688234772749 2314 68-69 -18.175957105682215 2314 70-71 -17.74446024514154 2314 72-73 -16.13535838224044 2314 74-75 -14.33791031900234 2314 76-77 -15.160357459907118 2314 78-79 -14.530912703191937 2314 80-81 -14.076444397516257 2314 82-83 -14.204809655542691 2314 84-85 -14.31335518631849 2314 86-87 -13.810918425987147 2314 88-89 -12.963481874377194 2314 90-91 -12.763018665449746 2314 92-93 -13.272187695463376 2314 94-95 -12.792622022421014 2314 96-97 -12.732774839696068 2314 98-99 -12.40244814483415 2314 100-101 -11.79991445399916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53627.0 3 9116.0 4 6407.0 5 5350.0 6 5400.0 7 6145.0 8 6909.0 9 8128.0 10 9199.0 11 9851.0 12 11192.0 13 12013.0 14 13368.0 15 15133.0 16 16410.0 17 17804.0 18 16114.0 19 14501.0 20 14652.0 21 15931.0 22 18549.0 23 22635.0 24 27598.0 25 33421.0 26 39722.0 27 46074.0 28 52295.0 29 58912.0 30 68500.0 31 78070.0 32 92173.0 33 111965.0 34 138181.0 35 171226.0 36 214967.0 37 263972.0 38 235313.0 39 59257.0 40 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75149201623762 19.53035750369628 14.034756025584851 23.68339445448125 2 14.953795815976708 20.363251844861338 40.81150856861201 23.87144377054994 3 18.271466182968645 25.361098639799295 30.366183268621388 26.001251908610673 4 11.903638148460342 17.190843332071708 39.31779878472039 31.587719734747555 5 13.01909899139031 37.23877049114558 35.49607027369671 14.246060243767403 6 30.46326429400375 37.243504636346216 18.3839922571592 13.909238812490834 7 26.65728372989755 32.151256811346414 22.7223563317055 18.469103127050538 8 25.048387605365356 35.34725207952835 20.95394781296032 18.650412502145972 9 25.35641782319353 16.43491082797447 21.20463318144325 37.00403816738875 10-11 24.06719275780351 26.358812910925266 29.153780537498008 20.420213793773208 12-13 25.060620227989183 23.946130051748302 28.7808406506045 22.212409069658015 14-15 22.26580783649988 25.546367868257974 26.525041787624254 25.662782507617887 16-17 21.82132497905716 28.560401601942637 27.12617688496193 22.49209653403828 18-19 21.771991921151077 27.712310226195747 28.906329834235322 21.60936801841785 20-21 22.654523798203844 26.93825053611957 28.78680582837668 21.62041983729991 22-23 22.356939565632914 26.784018402305694 28.64351813966944 22.215523892391957 24-25 22.18595410449722 26.94552942972173 28.5981378566585 22.270378609122552 26-27 22.025896968602623 27.001307545723403 28.694830949439478 22.277964536234492 28-29 22.032310588484975 27.149863929336355 28.638983031335048 22.17884245084363 30-31 22.292801831081526 26.908678213053467 28.60481660148545 22.193703354379558 32-33 22.12365043159914 27.04341765959284 28.511818146557278 22.321113762250747 34-35 22.393608471564246 27.12481366554486 28.474683801007572 22.00689406188332 36-37 22.311347688699872 26.893419676810247 28.467738725620144 22.327493908869734 38-39 22.242139243256666 27.102526516255192 28.467063571927063 22.188270668561078 40-41 22.350849068148428 26.934693437311452 28.472067726251126 22.24238976828899 42-43 22.289539252204534 27.004829007680964 28.348997910833535 22.356633829280963 44-45 22.30512365908594 27.184432010896952 28.213752597759058 22.29669173225805 46-47 22.359939947454023 27.088552483422994 28.080295258351057 22.471212310771925 48-49 22.298974195488903 27.27107573597232 28.084732843911258 22.34521722462752 50-51 22.167748114617638 27.40295866545303 27.95547540258437 22.47381781734497 52-53 22.37854276591278 27.459952973722686 27.739223103078725 22.42228115728581 54-55 22.32114825413734 27.296262526869263 27.796309168800658 22.586280050192737 56-57 22.253796452890175 27.40212942965486 27.68474248177299 22.65933163568198 58-59 22.276345920416844 27.436224883338888 27.770800143582907 22.516629052661365 60-61 22.378715530171196 27.36002363716945 27.777630323683994 22.483630508975363 62-63 22.256200297378292 27.437022097386894 27.728975784767712 22.577801820467105 64-65 22.223802662405646 27.34715365778298 27.75572383216025 22.673319847651122 66-67 22.24707596345467 27.315930937041593 27.72890079024231 22.708092309261424 68-69 22.272399114864616 27.27683379113325 27.71615172092437 22.734615373077766 70-71 22.289847841107598 27.129469548722945 27.65978083599897 22.920901774170485 72-73 22.36666723329197 27.201364300406073 27.66023080315137 22.77173766315059 74-75 22.199429441650757 27.382926046398616 27.633182777657233 22.784461734293394 76-77 22.27959858930298 27.286508084909798 27.552388675626677 22.88150465016054 78-79 22.328245038112218 27.32163052097197 27.444946518904118 22.905177922011692 80-81 22.342468999763017 27.324130338485293 27.434972247025968 22.898428414725725 82-83 22.45523576778895 27.22928726202987 27.524040745025612 22.791436225155564 84-85 22.45498578603762 27.289557862276055 27.387050745295593 22.868405606390734 86-87 22.323945351989305 27.301931958966996 27.47876904985936 22.89535363918434 88-89 22.399389844541346 27.199264453694884 27.40239962482739 22.998946076936384 90-91 22.480683910074564 27.20531401207712 27.382501077421352 22.93150100042697 92-93 22.521655919117904 27.09979671483982 27.43242243316238 22.9461249328799 94-95 22.33766935013744 27.08622270574248 27.506866998709096 23.069240945410986 96-97 22.384115959534952 27.064724275127915 27.410499033570552 23.14066073176658 98-99 22.36869208547776 27.06522423863058 27.291357730885647 23.274725945006015 100-101 22.47938400496764 26.97108111107889 27.265584612323302 23.28395027163017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 142.5 2 101.0 3 136.0 4 128.5 5 129.0 6 186.0 7 325.0 8 616.5 9 841.5 10 837.0 11 837.0 12 914.0 13 1042.5 14 1192.5 15 1354.5 16 1521.5 17 1650.0 18 1797.0 19 1978.0 20 2272.0 21 2702.5 22 3291.5 23 4375.0 24 5714.0 25 7408.0 26 9988.5 27 13280.5 28 16999.0 29 21297.5 30 25825.5 31 30556.0 32 35460.0 33 40022.0 34 44702.5 35 48893.5 36 53456.0 37 57848.0 38 60268.0 39 62319.0 40 65170.5 41 67457.0 42 68688.0 43 70146.0 44 71809.0 45 73559.5 46 74199.0 47 74067.5 48 74225.5 49 73684.0 50 72221.5 51 70611.5 52 68257.5 53 64885.0 54 61419.0 55 57286.0 56 52190.5 57 46996.0 58 42116.5 59 37014.5 60 31388.0 61 25743.0 62 20668.5 63 16310.0 64 12722.0 65 9668.5 66 7387.5 67 5777.5 68 4599.5 69 3738.5 70 2971.5 71 2295.5 72 1804.0 73 1488.0 74 1166.0 75 907.5 76 680.5 77 510.5 78 416.0 79 314.5 80 228.5 81 183.5 82 136.5 83 112.0 84 97.0 85 77.0 86 60.0 87 41.0 88 28.0 89 23.5 90 22.5 91 19.0 92 14.5 93 9.0 94 4.5 95 3.5 96 4.0 97 2.5 98 2.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0418969415232688 2 0.1894861675097718 3 0.0 4 0.5322111485861533 5 0.30567768552895636 6 0.09564301805968164 7 0.10964199613428219 8 0.11084190854067652 9 0.11049193408881153 10-11 0.125990802671405 12-13 0.22623348495559825 14-15 0.12724071142806576 16-17 0.12231607092682234 18-19 0.19108605071829757 20-21 0.09954273338046322 22-23 0.10676720599396244 24-25 0.09554302535914878 26-27 0.18286165109946975 28-29 0.11434165305932667 30-31 0.1318903720028438 32-33 0.10266750527211513 34-35 0.15323881356660965 36-37 0.13871487381421158 38-39 0.13459017491723105 40-41 0.10806711110088964 42-43 0.11099189759147582 44-45 0.08944347062664425 46-47 0.09479308010515232 48-49 0.10034267498472611 50-51 0.08724363121492132 52-53 0.09506806003161769 54-55 0.31157725486039517 56-57 0.33035088438543986 58-59 0.34359991720604394 60-61 0.16453798872682293 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2000146.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.91578207969049 #Duplication Level Percentage of deduplicated Percentage of total 1 86.86666437368956 66.8141742695632 2 8.581790167456841 13.20150204747482 3 2.1971425055906635 5.0698480247410975 4 0.8500635467000854 2.6153321004749026 5 0.4526074971162692 1.740632980791455 6 0.2565054546701156 1.1837590592195124 7 0.1733292929746502 0.9332230688525515 8 0.11904969127799554 0.7325440088794188 9 0.08672131929429377 0.6003214286852844 >10 0.3945915741485264 5.161350058607873 >50 0.01580660100256158 0.8267075502260591 >100 0.0054649435741809735 0.6529071510647767 >500 6.575812603960118E-5 0.033584042277740415 >1k 1.9727437811880352E-4 0.43411420914133425 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3975 0.19873549230906146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2922 0.14608933547851005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.999635026643055E-5 0.0 0.0 0.0 6 0.0 4.999635026643055E-5 0.0 0.0 0.0 7 0.0 4.999635026643055E-5 0.0 0.0 0.0 8 0.0 4.999635026643055E-5 0.0 0.0 0.0 9 0.0 4.999635026643055E-5 0.0 0.0 0.0 10-11 0.0 4.999635026643055E-5 0.0 4.999635026643055E-5 0.0 12-13 0.0 9.99927005328611E-5 0.0 4.999635026643055E-5 0.0 14-15 0.0 9.99927005328611E-5 0.0 7.499452539964582E-5 0.0 16-17 0.0 9.99927005328611E-5 0.0 9.99927005328611E-5 0.0 18-19 0.0 9.99927005328611E-5 0.0 9.99927005328611E-5 2.4998175133215276E-5 20-21 0.0 9.99927005328611E-5 4.999635026643055E-5 9.99927005328611E-5 4.999635026643055E-5 22-23 0.0 1.4998905079929164E-4 4.999635026643055E-5 1.999854010657222E-4 4.999635026643055E-5 24-25 0.0 1.4998905079929164E-4 4.999635026643055E-5 4.7496532753109024E-4 4.999635026643055E-5 26-27 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.0012499087566607637 4.999635026643055E-5 28-29 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.0028497919651865413 4.999635026643055E-5 30-31 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.007874425166962813 4.999635026643055E-5 32-33 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.02089847441136797 4.999635026643055E-5 34-35 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.04292186670373063 4.999635026643055E-5 36-37 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.07989416772575603 4.999635026643055E-5 38-39 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.12794066033179577 9.99927005328611E-5 40-41 0.0 1.4998905079929164E-4 4.999635026643055E-5 0.17818699234955848 9.99927005328611E-5 42-43 0.0 1.999854010657222E-4 4.999635026643055E-5 0.22995821305044734 9.99927005328611E-5 44-45 0.0 1.999854010657222E-4 4.999635026643055E-5 0.29707831328313034 9.99927005328611E-5 46-47 0.0 2.499817513321528E-4 4.999635026643055E-5 0.37844737334174605 9.99927005328611E-5 48-49 0.0 2.499817513321528E-4 4.999635026643055E-5 0.4659409863079995 9.99927005328611E-5 50-51 0.0 3.4997445186501385E-4 4.999635026643055E-5 0.5491849095016064 9.99927005328611E-5 52-53 0.0 3.4997445186501385E-4 4.999635026643055E-5 0.6387283728287836 9.99927005328611E-5 54-55 0.0 3.999708021314444E-4 4.999635026643055E-5 0.7374461664298506 9.99927005328611E-5 56-57 0.0 3.999708021314444E-4 4.999635026643055E-5 0.8554375530586267 9.99927005328611E-5 58-59 0.0 3.999708021314444E-4 4.999635026643055E-5 0.9856780455026783 9.99927005328611E-5 60-61 0.0 3.999708021314444E-4 4.999635026643055E-5 1.1196682642167122 1.4998905079929164E-4 62-63 0.0 3.999708021314444E-4 4.999635026643055E-5 1.254783400811741 1.7498722593250693E-4 64-65 0.0 3.999708021314444E-4 4.999635026643055E-5 1.39612308301494 1.999854010657222E-4 66-67 0.0 3.999708021314444E-4 4.999635026643055E-5 1.5584362341549067 2.249835761989375E-4 68-69 0.0 3.999708021314444E-4 4.999635026643055E-5 1.7432727410899003 2.7497992646536803E-4 70-71 0.0 4.249689772646597E-4 4.999635026643055E-5 1.93888346150731 2.999781015985833E-4 72-73 0.0 4.4996715239787493E-4 4.999635026643055E-5 2.1395938096518954 2.999781015985833E-4 74-75 0.0 4.4996715239787493E-4 4.999635026643055E-5 2.3398041942938166 2.999781015985833E-4 76-77 0.0 4.999635026643055E-4 4.999635026643055E-5 2.564587785091688 3.4997445186501385E-4 78-79 0.0 4.999635026643055E-4 4.999635026643055E-5 2.8098448813236634 3.4997445186501385E-4 80-81 0.0 5.499598529307361E-4 4.999635026643055E-5 3.0773253552490667 3.7497262699822916E-4 82-83 0.0 5.749580280639513E-4 4.999635026643055E-5 3.3556050408320193 3.999708021314444E-4 84-85 0.0 5.999562031971666E-4 4.999635026643055E-5 3.6332847702117745 3.999708021314444E-4 86-87 0.0 5.999562031971666E-4 4.999635026643055E-5 3.919813853588688 3.999708021314444E-4 88-89 0.0 6.499525534635972E-4 4.999635026643055E-5 4.235615800046596 3.999708021314444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2315 0.0 47.714073 1 GTATCAA 4225 0.0 42.101906 1 TCAACGC 4985 0.0 34.197758 4 ATCAACG 4915 0.0 34.19472 3 CAACGCA 5020 0.0 34.126987 5 TATCAAC 5300 0.0 32.56993 2 AACGCAG 5390 0.0 32.555233 6 ACGCAGA 6190 0.0 28.20544 7 CGCAGAG 6365 0.0 26.906096 8 GTACATG 5070 0.0 24.144459 1 GCAGAGT 6950 0.0 23.409271 9 TACATGG 5125 0.0 23.232235 2 ACATGGG 5190 0.0 21.895231 3 GAGTACT 4220 0.0 21.249609 12-13 GTATAGG 520 0.0 21.149982 1 CTATACG 210 8.186962E-7 20.465286 4 GTGGTAT 1265 0.0 20.034147 1 AGAGTAC 6460 0.0 19.919634 10-11 CAGAGTA 7065 0.0 18.819855 10-11 TCTAACG 205 1.5016833E-5 18.660429 2 >>END_MODULE