FastQCFastQC Report
Sat 18 Jun 2016
SRR1931110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000146
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.23243303238863564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38540.19268593392682334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.12179110924902482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.051.5727651
GTATCAA46450.039.2995831
CAACGCA56500.031.8541535
ATCAACG56200.031.77153
TCAACGC56550.031.7420164
TATCAAC59050.030.5597552
AACGCAG59850.030.467896
GTGGTAT10700.025.7683531
CGCAGAG70300.025.4660348
ACGCAGA70450.025.4118147
GCAGAGT77650.022.4439839
GAGTACT48000.021.36917712-13
GTACATG52050.020.8236351
TACATGG52150.020.2155152
TACACCG3302.7284841E-1120.1460275
CAGAGTA77100.020.07883610-11
AGAGTAC70400.019.96615210-11
GTACTTT54000.018.86291714-15
ACATGGG52900.018.76193
TATACCG2302.0647785E-618.5818945
Produced by FastQC (version 0.11.5)