FastQCFastQC Report
Sat 18 Jun 2016
SRR1931109_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931109_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212658
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50350.2275543712584593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.17454120790470104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.051.907511
GTATCAA49950.048.3253171
ATCAACG58000.040.2079283
CAACGCA59600.039.3439945
TCAACGC60200.038.9823954
TATCAAC62350.038.25542
AACGCAG62750.037.6508186
ACGCAGA69900.033.2600177
CGCAGAG71000.032.6762778
GCAGAGT80750.027.3184729
GTACATG57000.025.4927981
TACATGG57850.024.2098962
GTGGTAT13050.023.4416521
ACATGGG59650.022.5463
AGAGTAC72600.021.97034310-11
GAGTACT49500.021.94956412-13
CAGAGTA79100.020.8861910-11
GTACTTT55150.019.57221614-15
TGGTATC14500.018.790382
AGTACTT55800.018.57664112-13
Produced by FastQC (version 0.11.5)