FastQCFastQC Report
Sat 18 Jun 2016
SRR1931109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212658
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63060.28499659685319645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.23799430368362395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.14742450030687076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.054.230561
GTATCAA53050.041.142261
ATCAACG64750.033.2260863
CAACGCA67300.032.036275
TCAACGC67750.031.823484
TATCAAC69500.031.2285752
AACGCAG71200.030.3481716
GTGGTAT14150.027.8921871
ACGCAGA82350.025.835427
CGCAGAG83450.025.0965168
TGGTATC15300.024.8324362
GTACATG63900.023.2918721
TACATGG64400.022.1973632
GCAGAGT95900.021.6898549
GAGTACT58900.020.84233712-13
ACATGGG66450.020.6549243
CAGAGTA91200.019.8134910-11
AGAGTAC85200.019.23005910-11
GTACTTT64450.019.04753514-15
TATAGCG1507.735212E-418.9959555
Produced by FastQC (version 0.11.5)