##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931109_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2212658 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.270124438571166 31.0 31.0 34.0 30.0 34.0 2 31.2984053568152 31.0 31.0 34.0 28.0 34.0 3 31.449019685825824 31.0 31.0 34.0 28.0 34.0 4 35.082049733849516 35.0 35.0 37.0 32.0 37.0 5 34.969020969349984 35.0 35.0 37.0 32.0 37.0 6 35.08539819529272 36.0 35.0 37.0 32.0 37.0 7 34.93316454689337 36.0 35.0 37.0 32.0 37.0 8 34.984724254719886 36.0 35.0 37.0 32.0 37.0 9 36.398111682871914 38.0 35.0 39.0 32.0 39.0 10-11 36.2774267419547 38.0 35.0 39.0 32.0 39.0 12-13 36.18630940705703 38.0 35.0 39.0 32.0 39.0 14-15 37.14527075580591 39.0 36.0 40.0 31.5 41.0 16-17 37.161985946314346 39.0 36.0 40.0 31.0 41.0 18-19 37.23055732065235 39.0 36.0 40.0 31.5 41.0 20-21 37.208974003212425 39.0 36.0 40.0 31.0 41.0 22-23 37.09339197472 39.0 36.0 40.0 31.0 41.0 24-25 36.953742964344244 39.0 36.0 40.0 31.0 41.0 26-27 36.71013075676404 39.0 35.5 40.0 30.0 41.0 28-29 36.428899992678495 38.0 35.0 40.0 30.0 41.0 30-31 36.199791834074674 38.0 35.0 40.0 29.5 41.0 32-33 36.05769463694796 38.0 34.5 40.0 29.5 41.0 34-35 35.82665237917473 38.0 34.0 40.0 29.0 41.0 36-37 36.08734065544698 38.0 35.0 40.0 30.0 41.0 38-39 36.08624423656977 38.0 35.0 40.0 30.0 41.0 40-41 35.93144670346705 38.0 35.0 40.0 29.0 41.0 42-43 35.75683838171105 38.0 34.5 40.0 28.5 41.0 44-45 35.525990008397144 38.0 34.0 40.0 27.5 41.0 46-47 35.31677466648709 38.0 34.0 40.0 27.0 41.0 48-49 35.06151831869182 38.0 34.0 40.0 26.0 41.0 50-51 34.72247880151383 38.0 33.0 40.0 25.0 41.0 52-53 34.60036051662751 38.0 33.0 40.0 25.0 41.0 54-55 34.35677113227621 38.0 33.0 40.0 24.0 41.0 56-57 34.13585334922975 37.0 33.0 40.0 24.0 41.0 58-59 33.89394361894156 37.0 32.5 40.0 23.5 41.0 60-61 33.60581165277237 37.0 32.0 40.0 23.0 41.0 62-63 33.324429713041965 36.5 31.5 39.0 22.5 41.0 64-65 33.07152686949361 36.0 31.0 39.0 22.0 41.0 66-67 32.8228937775291 36.0 31.0 39.0 22.0 40.0 68-69 32.49174974171336 35.0 31.0 39.0 21.5 40.0 70-71 31.665982497069137 35.0 30.0 38.0 18.0 40.0 72-73 31.620075040968825 35.0 30.0 37.5 19.5 40.0 74-75 31.302949665063466 34.5 30.0 37.0 18.5 39.0 76-77 30.570365370518175 34.0 29.5 36.0 18.0 39.0 78-79 30.580105013969625 34.0 30.0 36.0 17.0 39.0 80-81 30.31203037252029 34.0 29.5 36.0 14.0 37.5 82-83 29.957127355425015 34.0 29.0 35.0 11.0 37.0 84-85 29.607604519089712 34.0 29.0 35.0 7.5 37.0 86-87 29.283029279716974 34.0 29.0 35.0 7.0 36.0 88-89 28.969843283507892 34.0 29.0 35.0 2.0 36.0 90-91 28.665931201297262 34.0 29.0 35.0 2.0 35.5 92-93 28.3129265797064 33.5 29.0 35.0 2.0 35.0 94-95 27.933559998879176 33.0 27.0 35.0 2.0 35.0 96-97 27.570082678841466 33.0 27.0 35.0 2.0 35.0 98-99 27.09111823878792 33.0 26.5 35.0 2.0 35.0 100-101 26.40867318853614 32.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.84782885714149 1101 2 0.720591339853307 1101 3 0.5723248170134099 1101 4 0.8692596455442398 1101 5 0.8840372265703564 1101 6 0.8078550706250596 1101 7 0.7009410861252903 1101 8 0.7834035019057737 1101 9 1.1447221008593473 1101 10-11 0.8964859331462876 1101 12-13 1.0586960541451944 1101 14-15 1.3969048742722805 1101 16-17 1.2014413516850198 1101 18-19 0.896374958485751 1101 20-21 0.928537728187159 1101 22-23 0.825582344889277 1101 24-25 0.702615242883887 1101 26-27 1.0033216359105026 1101 28-29 0.9696240814083623 1101 30-31 1.236970806233657 1101 32-33 1.295752364953799 1101 34-35 0.8334238897696693 1101 36-37 1.2745602821688564 1101 38-39 0.9767755394431319 1101 40-41 1.0084167255243273 1101 42-43 0.9941796260645717 1101 44-45 0.9960622568321327 1101 46-47 0.9877168511964314 1101 48-49 0.8948045239765605 1101 50-51 0.6814856622661836 1101 52-53 0.7680381294361212 1101 54-55 0.7537472038198558 1101 56-57 0.6193748329671749 1101 58-59 0.7953587082357245 1101 60-61 0.9229921591554486 1101 62-63 0.7168564245495475 1101 64-65 0.7423492775493585 1101 66-67 0.5098937408439568 1101 68-69 0.3911384083060412 1101 70-71 0.7873663504023796 1101 72-73 0.20444711516569924 1101 74-75 -0.07242351149548298 1101 76-77 0.015755534603549393 1101 78-79 0.19978350294172387 1101 80-81 0.18863272432926692 1101 82-83 0.2795830642199597 1101 84-85 0.3577207733970198 1101 86-87 0.22504638153283096 1101 88-89 0.11349280385585914 1101 90-91 0.21942211908722697 1101 92-93 -0.013885331091753272 1101 94-95 -0.08078647795384697 1101 96-97 0.0017929181707820874 1101 98-99 -0.07006679209637667 1101 100-101 -0.15212840973353536 1102 1 0.9202169307061894 1102 2 0.85010440794888 1102 3 0.6601228110722879 1102 4 0.9401753325141797 1102 5 0.9615702509275863 1102 6 0.7733767201771329 1102 7 0.8825925276403481 1102 8 0.9241024917000757 1102 9 1.4169857227727078 1102 10-11 1.0645424402823025 1102 12-13 1.22824389121876 1102 14-15 1.661526629491533 1102 16-17 1.3812135830421397 1102 18-19 1.143343779210106 1102 20-21 1.1621305018029702 1102 22-23 0.7586017707373358 1102 24-25 0.7205474694697287 1102 26-27 1.005778098609099 1102 28-29 1.1141532012371016 1102 30-31 1.5135324893019941 1102 32-33 1.3783748238876825 1102 34-35 1.2161609009305963 1102 36-37 1.5326773823366153 1102 38-39 1.3430563465126468 1102 40-41 1.431727932625904 1102 42-43 1.5411281957444203 1102 44-45 1.476326440690599 1102 46-47 1.444588817664517 1102 48-49 1.287016323014825 1102 50-51 1.1475431028812437 1102 52-53 1.1038644576027679 1102 54-55 0.9094954878585355 1102 56-57 1.0801459504557442 1102 58-59 1.106937160439955 1102 60-61 1.426109133441635 1102 62-63 1.2432999256019457 1102 64-65 1.3207619757486668 1102 66-67 0.942822605292001 1102 68-69 0.8725117554382749 1102 70-71 1.2349287332744723 1102 72-73 0.7604671628442574 1102 74-75 0.730274546041926 1102 76-77 0.771095601826751 1102 78-79 0.831219809190312 1102 80-81 0.7380347929911828 1102 82-83 0.7614982215095871 1102 84-85 0.8093387426364522 1102 86-87 0.7569445264784918 1102 88-89 0.6234136157944157 1102 90-91 0.6837547988956025 1102 92-93 0.4381146825963391 1102 94-95 0.5825458344758978 1102 96-97 0.562938362223516 1102 98-99 0.47002520410908843 1102 100-101 0.266840372126417 1103 1 0.6427311356703385 1103 2 0.4384886749501291 1103 3 0.3238048099954334 1103 4 0.618135371442122 1103 5 0.7440832965033408 1103 6 0.6215882776091064 1103 7 0.5912818243239073 1103 8 0.5789494966461461 1103 9 0.8634759568905608 1103 10-11 0.7218165155226828 1103 12-13 0.9992001434662967 1103 14-15 1.322492509329777 1103 16-17 1.0311927533523715 1103 18-19 0.8025996532808719 1103 20-21 0.8759525484575406 1103 22-23 0.6106980161857507 1103 24-25 0.47322729625906845 1103 26-27 0.8524334473197257 1103 28-29 0.6265405723042718 1103 30-31 1.1325479143259258 1103 32-33 1.0882569150706303 1103 34-35 0.8611604428248043 1103 36-37 1.2500578547972125 1103 38-39 0.9842045450883674 1103 40-41 0.889856418160079 1103 42-43 0.9721328880741851 1103 44-45 1.084136419880565 1103 46-47 1.0273225093438398 1103 48-49 1.0242140161758115 1103 50-51 0.8634743091462624 1103 52-53 0.4680493842272426 1103 54-55 0.6557012235396016 1103 56-57 0.7597160531696971 1103 58-59 0.9297870660677034 1103 60-61 0.9617799771816209 1103 62-63 0.7935743346577482 1103 64-65 0.8602821363337796 1103 66-67 0.6896249224475994 1103 68-69 0.4851113351108509 1103 70-71 0.9897600299816105 1103 72-73 0.42125560344722146 1103 74-75 0.2991083499264704 1103 76-77 0.4372197467778136 1103 78-79 0.5158763600294982 1103 80-81 0.5227178230822283 1103 82-83 0.3742699593050638 1103 84-85 0.365660823679665 1103 86-87 0.2677121619549929 1103 88-89 0.22636558567389997 1103 90-91 0.23017418013591495 1103 92-93 -0.13375066402149116 1103 94-95 -0.22267709970032 1103 96-97 -0.1820474672009773 1103 98-99 -0.3534531538923602 1103 100-101 -0.45666281324406555 1104 1 0.5034608661937519 1104 2 0.4159609430672013 1104 3 0.2009005878938872 1104 4 0.6957189907356707 1104 5 0.3861020817789864 1104 6 0.545443004521907 1104 7 0.5329694084510948 1104 8 0.5182959620045224 1104 9 0.9252298245747497 1104 10-11 0.682182711895507 1104 12-13 0.7511814650646755 1104 14-15 1.1860899960090308 1104 16-17 0.9728640897397938 1104 18-19 0.7730014466462123 1104 20-21 0.7944099567528937 1104 22-23 0.427061307462715 1104 24-25 0.5641481736177596 1104 26-27 0.3134958593412307 1104 28-29 0.5760860147049556 1104 30-31 0.8763615272359218 1104 32-33 1.015534800043369 1104 34-35 0.6798581431558262 1104 36-37 1.0350504915622523 1104 38-39 0.6667247031174881 1104 40-41 0.8158775404624734 1104 42-43 0.8826669055456762 1104 44-45 0.9658939150230879 1104 46-47 0.9041415641634742 1104 48-49 0.8571473843154109 1104 50-51 0.6598360009235122 1104 52-53 0.3752253549514606 1104 54-55 0.6249540309083272 1104 56-57 0.7324925439660106 1104 58-59 0.7211463796572701 1104 60-61 0.8276367120743515 1104 62-63 0.07196882448353392 1104 64-65 -0.8443943723711556 1104 66-67 -0.35220136321799345 1104 68-69 0.14007421323788805 1104 70-71 0.8064707821821528 1104 72-73 0.32103370746356674 1104 74-75 0.20628732949138495 1104 76-77 0.06981333857297045 1104 78-79 -0.01466999248531664 1104 80-81 0.10803711162860452 1104 82-83 -0.1555665776011388 1104 84-85 -0.17812346506890364 1104 86-87 0.09112649087279223 1104 88-89 0.23389418651001748 1104 90-91 0.3151242237520684 1104 92-93 0.32144315466432616 1104 94-95 0.3821411013306779 1104 96-97 0.4700080885150477 1104 98-99 0.3677653291031149 1104 100-101 0.10735604953931954 1105 1 -0.3504883140943882 1105 2 -1.8085495542239265 1105 3 -0.4645959431198854 1105 4 0.36603046141398465 1105 5 0.3927959363084952 1105 6 -0.15459445039449093 1105 7 -0.22845907799573695 1105 8 -0.31403512183059945 1105 9 0.5136082249854326 1105 10-11 0.8789512339024768 1105 12-13 0.9660578968890121 1105 14-15 1.289494427586611 1105 16-17 0.8400256019489944 1105 18-19 0.44115661575516185 1105 20-21 0.9505579609566581 1105 22-23 0.2107235830912444 1105 24-25 -0.13455280175232787 1105 26-27 0.860047803802189 1105 28-29 0.4038628010290637 1105 30-31 0.8846063457888818 1105 32-33 1.0425364899210763 1105 34-35 0.5464554080656328 1105 36-37 0.6974475180825408 1105 38-39 0.588372763503628 1105 40-41 0.8332045152729393 1105 42-43 1.1920197286549197 1105 44-45 1.2688367698368168 1105 46-47 1.1315888573847985 1105 48-49 1.0417386520321372 1105 50-51 0.5463625862028465 1105 52-53 0.7529283330579659 1105 54-55 0.03960330968085657 1105 56-57 0.20691876769524953 1105 58-59 -0.5435316512716781 1105 60-61 0.2633796083088171 1105 62-63 0.716816129602293 1105 64-65 1.198437417599294 1105 66-67 0.7332623837277836 1105 68-69 0.6471159578438233 1105 70-71 0.2925021914344015 1105 72-73 -0.24202869463038468 1105 74-75 -0.2731782329162158 1105 76-77 -0.09394054737460777 1105 78-79 -0.4582129852212695 1105 80-81 -0.372459342272105 1105 82-83 -0.34375197503085175 1105 84-85 -0.17761936595879035 1105 86-87 -0.38262818823772093 1105 88-89 -0.8007849900987232 1105 90-91 -0.8712525418616757 1105 92-93 -0.5070244545442968 1105 94-95 -0.8095731000313577 1105 96-97 -0.9627116451584641 1105 98-99 -1.485282399476528 1105 100-101 -0.9571490278050838 1106 1 0.31059362570916704 1106 2 0.41016908030631427 1106 3 -0.18401670955494964 1106 4 0.4433106852879831 1106 5 0.06785383693410552 1106 6 0.3155937361099319 1106 7 0.3554019908106625 1106 8 0.44417085538295 1106 9 0.8027835230757816 1106 10-11 0.1018571507528847 1106 12-13 0.3229146695573917 1106 14-15 0.17831184045785164 1106 16-17 -0.09523354281393637 1106 18-19 0.44286825169724153 1106 20-21 0.5068866193038204 1106 22-23 0.4169001970475037 1106 24-25 0.31930627178714843 1106 26-27 0.7233193596305227 1106 28-29 0.6207925145945978 1106 30-31 0.24214062523232371 1106 32-33 0.20907308538954084 1106 34-35 -1.2913316641200865 1106 36-37 -0.9380659600718175 1106 38-39 -0.8424283487945345 1106 40-41 0.36711256124995373 1106 42-43 0.5010392191294102 1106 44-45 0.21612207027602892 1106 46-47 -0.20242930712117158 1106 48-49 -0.16341343024357968 1106 50-51 0.4014837292129698 1106 52-53 -0.23343022903651445 1106 54-55 0.4344279913852773 1106 56-57 0.10267667867603336 1106 58-59 0.5857891750213895 1106 60-61 0.7463537935958655 1106 62-63 0.5468687642386172 1106 64-65 0.5724139593218496 1106 66-67 0.38616271888211173 1106 68-69 0.3924825186855756 1106 70-71 0.6797118990394004 1106 72-73 0.075735495127077 1106 74-75 0.11185789656902756 1106 76-77 0.18422201384094095 1106 78-79 0.17690585944341208 1106 80-81 0.11793875136400445 1106 82-83 0.16907001432604218 1106 84-85 0.22835077575567553 1106 86-87 0.1290211398119432 1106 88-89 0.2137121394951258 1106 90-91 0.34316793412314084 1106 92-93 -0.05334583900697609 1106 94-95 0.23987959067448728 1106 96-97 0.35579028822992953 1106 98-99 0.2963870327816309 1106 100-101 0.12835826249614968 1107 1 0.40208031003023237 1107 2 0.3030698947592505 1107 3 0.11940986645579343 1107 4 -1.2766684317308972 1107 5 0.038087114503539965 1107 6 0.20954689610562838 1107 7 0.49455541320507734 1107 8 0.5234524152605076 1107 9 0.8948800815793874 1107 10-11 0.8113135764233945 1107 12-13 0.9303017740664927 1107 14-15 1.141754120381485 1107 16-17 1.173186463504308 1107 18-19 0.7877905142721104 1107 20-21 0.20262226510222803 1107 22-23 0.43613839334252447 1107 24-25 0.42063846483635814 1107 26-27 0.7677163246520848 1107 28-29 0.7396440728856533 1107 30-31 1.0965101723111417 1107 32-33 1.186404589933872 1107 34-35 0.8140064576285795 1107 36-37 1.0167835538209573 1107 38-39 0.7168051602214618 1107 40-41 0.17147673198915925 1107 42-43 0.025340413065791267 1107 44-45 -0.22184156955312062 1107 46-47 0.1816707033536602 1107 48-49 0.48571344942616435 1107 50-51 -1.230644053737393 1107 52-53 0.3679216216885095 1107 54-55 0.7645992173824254 1107 56-57 0.7052846757241795 1107 58-59 0.8004034136247355 1107 60-61 1.066877407396305 1107 62-63 1.1954678735166766 1107 64-65 1.1571584776087604 1107 66-67 0.7571499482000874 1107 68-69 0.7651905239857868 1107 70-71 1.3321302238275479 1107 72-73 0.832906795865064 1107 74-75 0.5899583427079698 1107 76-77 0.5640194939352838 1107 78-79 0.6120340705560174 1107 80-81 0.6835366934067864 1107 82-83 0.7449294715296837 1107 84-85 0.5819636065082285 1107 86-87 0.6666085608999204 1107 88-89 0.7172380874882798 1107 90-91 0.8114120355006236 1107 92-93 0.7402973965436601 1107 94-95 0.7441034208899602 1107 96-97 0.7415586310642404 1107 98-99 0.5919656116664278 1107 100-101 0.6363733845974053 1108 1 0.4567535891634513 1108 2 0.490267190807959 1108 3 0.29688904784717707 1108 4 0.6034890355259392 1108 5 0.6109228281405663 1108 6 0.497705658479326 1108 7 0.5074082634492925 1108 8 0.4860270917774301 1108 9 0.6272054875866786 1108 10-11 0.5253673711552693 1108 12-13 0.7985639088770995 1108 14-15 1.2187922513067448 1108 16-17 0.7833071304894901 1108 18-19 0.4668556215338455 1108 20-21 0.5511968468545092 1108 22-23 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1.2088384945980124 2311 68-69 1.538754120070216 2311 70-71 2.007227344394849 2311 72-73 1.437197590478256 2311 74-75 1.2936658839279005 2311 76-77 1.4964201341625483 2311 78-79 1.561426172653185 2311 80-81 1.6139042940915296 2311 82-83 1.507788870627646 2311 84-85 1.5779923955353254 2311 86-87 1.2074988371834223 2311 88-89 1.3937863191784672 2311 90-91 1.5760471642615634 2311 92-93 1.5257717106048112 2311 94-95 1.6570028341916334 2311 96-97 1.881421257797971 2311 98-99 2.104015186879881 2311 100-101 2.0157597098095366 2312 1 -0.029326188112985818 2312 2 -0.273060960904008 2312 3 -0.3331826948391665 2312 4 0.1734767399445971 2312 5 -1.4346457778802062 2312 6 -0.08485492402845551 2312 7 0.31683561428690865 2312 8 -2.361301665482685 2312 9 -5.0518909164173245 2312 10-11 -0.5629126874795105 2312 12-13 -5.504121364856509 2312 14-15 -1.9986054424409687 2312 16-17 -3.1209804458040367 2312 18-19 -2.3102531814377656 2312 20-21 -3.4852501362972177 2312 22-23 -2.341177854954772 2312 24-25 -3.099238711016554 2312 26-27 -3.282352288597899 2312 28-29 -5.747271187454327 2312 30-31 -6.49719375830038 2312 32-33 -1.5781277331527903 2312 34-35 0.6556046870944314 2312 36-37 1.0833423100014343 2312 38-39 0.8674332883472786 2312 40-41 -2.5217692258453823 2312 42-43 -4.631136374621665 2312 44-45 -3.79414335579391 2312 46-47 0.4889666707867093 2312 48-49 -0.83317834095714 2312 50-51 -6.313598115926531 2312 52-53 -1.2976964808198659 2312 54-55 0.8921213513555273 2312 56-57 1.589226098195347 2312 58-59 1.4278713550710904 2312 60-61 1.7070165260421177 2312 62-63 1.832411010859829 2312 64-65 1.7739206394680593 2312 66-67 1.4267011422267828 2312 68-69 1.4258906546908108 2312 70-71 2.030247768336608 2312 72-73 1.3186564576210387 2312 74-75 1.180286492235254 2312 76-77 1.241465804296805 2312 78-79 1.0469623940504995 2312 80-81 1.0939447355777538 2312 82-83 0.1409900422412882 2312 84-85 0.7347566801521985 2312 86-87 1.1899751843656539 2312 88-89 1.2478084133139582 2312 90-91 1.4567997922147562 2312 92-93 1.6343397548922027 2312 94-95 1.747356350271584 2312 96-97 1.9845262321998973 2312 98-99 1.8950660212333403 2312 100-101 1.995040882216152 2313 1 -0.2048855684916404 2313 2 -0.06109220676657756 2313 3 -0.3322559107798533 2313 4 -1.3445955492120305 2313 5 -1.988862645350082 2313 6 -1.548776544576583 2313 7 -0.9717914213280778 2313 8 -4.201762409557887 2313 9 -2.5780394666622612 2313 10-11 -1.0068422518910083 2313 12-13 -2.9322294013982884 2313 14-15 -1.7338761957840063 2313 16-17 -5.543329181405788 2313 18-19 -2.6809006063020604 2313 20-21 -3.6204944100957093 2313 22-23 -1.343163820796157 2313 24-25 -0.13465510185462648 2313 26-27 -2.8318028380484463 2313 28-29 -3.5075651103988505 2313 30-31 -5.847319536137 2313 32-33 -8.413424966040385 2313 34-35 -2.4064898448796157 2313 36-37 -4.553689333054308 2313 38-39 -0.7893918142609451 2313 40-41 0.05866239073081658 2313 42-43 -1.9404836538484602 2313 44-45 -2.896486795486748 2313 46-47 0.8903965661925071 2313 48-49 1.4625319728254595 2313 50-51 2.3873816272218846 2313 52-53 2.476300606430236 2313 54-55 -2.256826086748532 2313 56-57 -3.38614792537145 2313 58-59 0.8913295835895596 2313 60-61 -7.0004507055215015 2313 62-63 -1.2601349973488283 2313 64-65 0.4715242406861222 2313 66-67 -0.7620318115664162 2313 68-69 -3.5346548239269566 2313 70-71 -4.3604579115257 2313 72-73 0.19731394471225627 2313 74-75 1.8847747750367887 2313 76-77 1.37148036804631 2313 78-79 2.465868788860508 2313 80-81 2.580837361024596 2313 82-83 2.3460741147952398 2313 84-85 3.319027830688402 2313 86-87 3.288876283266749 2313 88-89 -0.5980806373943715 2313 90-91 2.2263615557524226 2313 92-93 3.3606074630876215 2313 94-95 3.705783465325208 2313 96-97 4.162005379558561 2313 98-99 3.5868441226499925 2313 100-101 3.9707459000898453 2314 1 -28.776314139920213 2314 2 -14.756147151821523 2314 3 -4.3659097569337 2314 4 -3.631454523571005 2314 5 -1.121074183811622 2314 6 -0.5198159218659626 2314 7 0.30453641749976157 2314 8 -3.7340426293381057 2314 9 -2.3060614446842393 2314 10-11 -0.06173007240383299 2314 12-13 0.6513901040962224 2314 14-15 -0.8575025694103857 2314 16-17 -0.21446013378674422 2314 18-19 -3.853691357217812 2314 20-21 -2.513237084088388 2314 22-23 -6.663047062554405 2314 24-25 -0.8430456696202029 2314 26-27 -4.4934901091107164 2314 28-29 -3.4585152019739382 2314 30-31 -4.6140892247816865 2314 32-33 -1.4630472187876364 2314 34-35 -1.570352024366791 2314 36-37 -5.040296475911454 2314 38-39 -6.856756741000879 2314 40-41 -5.290753818060395 2314 42-43 -2.7509232142880222 2314 44-45 -4.6193576746076275 2314 46-47 -5.403902884356945 2314 48-49 -4.400162624244139 2314 50-51 -1.8631907171004585 2314 52-53 -3.312790510785881 2314 54-55 -4.9503929365653825 2314 56-57 -1.3646277788513004 2314 58-59 -1.2277752698902944 2314 60-61 0.309015869570068 2314 62-63 1.4475687572299307 2314 64-65 -0.2872326274457464 2314 66-67 0.0843509723163649 2314 68-69 0.9864646815675471 2314 70-71 1.6015956599028698 2314 72-73 1.0541706296939424 2314 74-75 1.2427189142309345 2314 76-77 -0.7507723792064382 2314 78-79 -2.468826723960003 2314 80-81 -2.1901676756054407 2314 82-83 -1.9993356837395595 2314 84-85 -0.2161141107035327 2314 86-87 0.8261931330835992 2314 88-89 0.8746185384298037 2314 90-91 1.0366866473275174 2314 92-93 0.9937919868971434 2314 94-95 1.2315961942629379 2314 96-97 1.2998258923790722 2314 98-99 1.7952919248477954 2314 100-101 1.8500957169396628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 54.0 5 115.0 6 221.0 7 550.0 8 1419.0 9 2462.0 10 3780.0 11 5334.0 12 7084.0 13 8865.0 14 11064.0 15 13034.0 16 15527.0 17 17416.0 18 16207.0 19 13966.0 20 13905.0 21 14654.0 22 15816.0 23 18503.0 24 21591.0 25 26708.0 26 33587.0 27 42749.0 28 53633.0 29 66605.0 30 81825.0 31 98917.0 32 119521.0 33 144894.0 34 177261.0 35 216868.0 36 269142.0 37 332349.0 38 287403.0 39 59574.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.677703796687055 20.882548054775974 15.13203920223506 23.307708946301915 2 15.345209246074178 21.518463314258234 39.83186737399092 23.304460065676665 3 18.61627289470413 25.944205281176906 30.577494548207408 24.86202727591155 4 13.074033750434433 17.750908072797678 38.159801830653066 31.01525634611482 5 12.587112659378882 38.18476651547555 35.721658896202385 13.506461928943187 6 30.54968277971562 38.270306572457194 17.671190034790733 13.508820613036448 7 26.5501491870863 32.894464485700006 22.44314304334425 18.112243283869446 8 25.005355549750576 36.397445967700385 20.267253231181684 18.32994525136736 9 25.384944261607533 16.319150993962918 20.89021439372917 37.405690350700375 10-11 24.183131780871694 26.97662720583118 28.696956330350194 20.143284682946934 12-13 24.885906452782127 24.37837207557607 28.730535853258843 22.005185618382956 14-15 22.173580372565485 26.043473505620838 26.478357703721045 25.304588418092628 16-17 21.62478340529806 29.065992123500333 27.15948420406588 22.149740267135726 18-19 21.561850046414765 28.06929493848575 29.16740860991622 21.20144640518327 20-21 22.29088273018243 27.159732773885526 29.253775323615304 21.295609172316734 22-23 22.120996557082027 26.979790821717593 28.994720377030703 21.90449224416968 24-25 21.97806420087256 27.1893251533465 29.083071662805178 21.749538982975764 26-27 21.920051706389966 27.33882713726713 28.902516053726735 21.838605102616167 28-29 21.77916876365396 27.418605066351425 29.025814469110617 21.776411700883997 30-31 22.24706735755979 27.141388279373313 29.004404330661508 21.60714003240539 32-33 22.110273871223242 27.264711299122872 28.85413382957592 21.770881000077964 34-35 22.225694781060437 27.29264021661536 28.94514132900175 21.536523673322456 36-37 22.148737210018616 27.183694628156974 28.951756525802775 21.71581163602163 38-39 22.06663594913665 27.29472490824851 28.917089559979292 21.72154958263554 40-41 22.201406951597342 27.087818095124028 28.92684348849875 21.783931464779887 42-43 22.061560176180635 27.239120398460553 28.701168368385744 21.99815105697307 44-45 22.148403850968844 27.28747314291809 28.67432449411065 21.889798512002415 46-47 22.172945042519558 27.222711879502565 28.457190754677786 22.147152323300094 48-49 22.113677775552794 27.377406652855708 28.460781836435523 22.048133735155968 50-51 22.21612128344655 27.422473476369692 28.2013231398406 22.160082100343157 52-53 22.175922358442747 27.51723684421635 28.106241917139762 22.200598880201134 54-55 22.148117673121178 27.38938193128953 28.194530469417558 22.267969926171734 56-57 22.225313054061736 27.488648160543278 28.020005088950423 22.26603369644456 58-59 22.11422344831576 27.55725318490049 28.173872878766787 22.154650488016962 60-61 22.415741598000707 27.529175735062655 28.011349628160925 22.043733038775713 62-63 22.308190159731932 27.463208143246217 28.033333905816825 22.195267791205026 64-65 22.07892994909954 27.364075797230726 28.10822635754708 22.448767896122657 66-67 22.150788404716305 27.360327226092213 28.024677547838174 22.46420682135331 68-69 22.12158423144473 27.44311458976429 27.926662044665886 22.5086391341251 70-71 22.159426417611712 27.295602970418077 27.905012789538592 22.639957822431622 72-73 22.19433850893218 27.361154173306783 27.862993217817174 22.581514099943867 74-75 22.12655950936327 27.465668761454104 27.79504979357192 22.612721935610708 76-77 22.00371679672141 27.430967641632826 27.71939902144841 22.845916540197354 78-79 21.99235037678665 27.511210498866067 27.66399054892351 22.832448575423765 80-81 22.0804189364746 27.584767680209776 27.5325000491492 22.80231333416642 82-83 22.090615057857214 27.521715521080985 27.74981351508861 22.63785590597319 84-85 22.16759209963763 27.551682184955833 27.472817760358808 22.807907955047728 86-87 21.89193898029471 27.679216063963715 27.55416123666434 22.874683719077233 88-89 21.99805159191178 27.47214727813808 27.52073319079959 23.00906793915055 90-91 22.078132792619463 27.40385256372449 27.58718638290487 22.930828260751174 92-93 22.10434140041603 27.46151855798119 27.470444738468565 22.96369530313422 94-95 21.90975054742175 27.445997889403856 27.46970255056731 23.174549012607084 96-97 21.8479227641762 27.46780786133563 27.460893082147575 23.223376292340596 98-99 21.70859317782097 27.47774528991967 27.38003314168284 23.433628390576516 100-101 21.785207448250926 27.514078459730634 27.276710657145443 23.424003434873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 352.0 1 241.0 2 147.5 3 174.5 4 154.0 5 159.5 6 281.5 7 569.0 8 938.0 9 1193.5 10 1263.0 11 1206.0 12 1244.5 13 1420.5 14 1644.0 15 1834.0 16 2012.5 17 2178.0 18 2477.0 19 2813.0 20 3104.5 21 3551.5 22 4254.0 23 5356.5 24 6840.0 25 8824.0 26 11649.5 27 14924.0 28 18542.5 29 23140.5 30 28129.0 31 33337.5 32 38867.0 33 44620.0 34 50067.0 35 55264.0 36 61461.0 37 66908.0 38 70220.5 39 72393.0 40 73992.0 41 75414.0 42 76191.0 43 77679.0 44 79995.5 45 82401.5 46 83203.5 47 83179.5 48 83980.0 49 83460.0 50 81995.0 51 79527.0 52 76086.5 53 72187.0 54 67911.5 55 62671.5 56 56919.5 57 50534.5 58 43961.5 59 37922.0 60 31215.0 61 24802.5 62 19374.5 63 14996.5 64 11531.5 65 8625.5 66 6519.5 67 5063.5 68 4033.5 69 3224.5 70 2528.5 71 1953.5 72 1489.5 73 1150.0 74 861.0 75 620.5 76 461.0 77 374.0 78 286.0 79 198.0 80 144.5 81 101.5 82 76.0 83 65.0 84 47.5 85 33.5 86 26.5 87 21.5 88 15.5 89 9.0 90 8.0 91 4.0 92 1.5 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1258215232539326 2 0.0 3 0.0037511445510331923 4 2.259725633152525E-4 5 2.71167075978303E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.019411043188780192 26-27 0.007479691845734858 28-29 0.007163330257093504 30-31 0.0028472542977721815 32-33 0.0013784326362230404 34-35 0.003276602168071161 36-37 0.001694794224864394 38-39 0.001062071047581687 40-41 0.0032991994244026867 42-43 0.0019207667881796463 44-45 9.0389025326101E-4 46-47 0.03586184579813058 48-49 0.018303777628535453 50-51 0.0770566440905011 52-53 0.0012880436108969394 54-55 0.03470938572522279 56-57 9.490847659240606E-4 58-59 0.001355835379891515 60-61 0.0033669911933972623 62-63 0.003434782962391838 64-65 0.009151888814267727 66-67 0.005445938775897586 68-69 0.002350114658478626 70-71 0.004858410111277929 72-73 0.004338673215652848 74-75 0.0010394737912501617 76-77 0.0 78-79 0.0 80-81 3.3895884497287876E-4 82-83 5.649314082881312E-4 84-85 0.0 86-87 0.0012880436108969394 88-89 0.0037285472947016667 90-91 0.0012428490982338888 92-93 0.002960240579429808 94-95 5.875286646196565E-4 96-97 2.71167075978303E-4 98-99 0.0016495997122013433 100-101 0.002621281734456929 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2212658.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.80036048658637 #Duplication Level Percentage of deduplicated Percentage of total 1 84.0612483876002 59.51566688794572 2 10.433618294931913 14.774078729212443 3 2.6160731739667558 5.556567713284035 4 1.0085344422715585 2.856184083038587 5 0.508815533426037 1.8012161593869078 6 0.30027346498161833 1.2755681739152969 7 0.20227183327842183 1.0024643098676476 8 0.1405480359416036 0.7960681288277784 9 0.1080725558983673 0.6886418324680044 >10 0.566952379334362 7.380874645845004 >50 0.039079815488013946 1.8700933884969837 >100 0.013999192831515824 1.6319939891862618 >500 2.5644502483575054E-4 0.11638252530350339 >1k 1.2822251241759557E-4 0.20893477533974086 >5k 1.2822251241759557E-4 0.525264657882138 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6306 0.28499659685319645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.23799430368362395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3262 0.14742450030687076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.51945126630505E-5 9 0.0 1.355835379891515E-4 0.0 0.0 4.51945126630505E-5 10-11 0.0 1.355835379891515E-4 0.0 4.51945126630505E-5 4.51945126630505E-5 12-13 0.0 1.355835379891515E-4 0.0 9.0389025326101E-5 4.51945126630505E-5 14-15 0.0 2.0337530698372728E-4 0.0 9.0389025326101E-5 4.51945126630505E-5 16-17 0.0 2.485698196467778E-4 0.0 9.0389025326101E-5 4.51945126630505E-5 18-19 0.0 3.3895884497287876E-4 0.0 9.0389025326101E-5 4.51945126630505E-5 20-21 0.0 4.067506139674545E-4 0.0 1.355835379891515E-4 1.355835379891515E-4 22-23 0.0 4.067506139674545E-4 0.0 2.2597256331525253E-4 1.355835379891515E-4 24-25 0.0 4.5194512663050506E-4 0.0 2.71167075978303E-4 1.355835379891515E-4 26-27 0.0 4.971396392935556E-4 0.0 6.32723177282707E-4 1.5818079432067676E-4 28-29 0.0 4.971396392935556E-4 0.0 0.0019659613008426967 1.80778050652202E-4 30-31 0.0 4.971396392935556E-4 0.0 0.007163330257093505 1.80778050652202E-4 32-33 0.0 4.971396392935556E-4 0.0 0.02099285113198696 1.80778050652202E-4 34-35 0.0 5.197368956250809E-4 0.0 0.04313816233688171 2.2597256331525253E-4 36-37 0.0 5.875286646196565E-4 0.0 0.07780235354944144 2.2597256331525253E-4 38-39 0.0 5.875286646196565E-4 0.0 0.12692878881417735 2.2597256331525253E-4 40-41 0.0 5.875286646196565E-4 0.0 0.17944481252864203 2.2597256331525253E-4 42-43 0.0 5.875286646196565E-4 0.0 0.2328421292400362 2.2597256331525253E-4 44-45 0.0 6.101259209511818E-4 0.0 0.3014022049498838 2.71167075978303E-4 46-47 0.0 6.32723177282707E-4 0.0 0.3838595933036194 2.71167075978303E-4 48-49 0.0 6.32723177282707E-4 0.0 0.46986475090140456 2.71167075978303E-4 50-51 0.0 7.23112202608808E-4 0.0 0.5600504009205218 3.163615886413535E-4 52-53 0.0 7.23112202608808E-4 0.0 0.6525635683417863 3.163615886413535E-4 54-55 0.0 7.683067152718585E-4 0.0 0.7556070572135414 3.163615886413535E-4 56-57 0.0 7.683067152718585E-4 0.0 0.8714179959126083 3.3895884497287876E-4 58-59 0.0 8.13501227934909E-4 0.0 1.00137481707521 3.61556101304404E-4 60-61 0.0 8.13501227934909E-4 0.0 1.1287781482723496 3.61556101304404E-4 62-63 0.0 8.13501227934909E-4 0.0 1.2617178072707125 3.61556101304404E-4 64-65 0.0 8.360984842664342E-4 0.0 1.4038093550833433 3.8415335763592926E-4 66-67 0.0 9.038902532610101E-4 0.0 1.5644758476004879 4.067506139674545E-4 68-69 0.0 9.038902532610101E-4 0.0 1.7452764955090214 4.2934787029897975E-4 70-71 0.0 9.490847659240606E-4 0.0 1.9380536892732634 4.5194512663050506E-4 72-73 0.0 9.94279278587111E-4 0.0 2.1427170398678874 4.5194512663050506E-4 74-75 0.0 9.94279278587111E-4 0.0 2.347131820642865 4.5194512663050506E-4 76-77 0.0 9.94279278587111E-4 0.0 2.5690594750747744 4.5194512663050506E-4 78-79 0.0 9.94279278587111E-4 0.0 2.8144656788351385 4.5194512663050506E-4 80-81 0.0 9.94279278587111E-4 0.0 3.077633326072082 4.971396392935556E-4 82-83 0.0 9.94279278587111E-4 0.0 3.3524611575760916 5.42334151956606E-4 84-85 0.0 9.94279278587111E-4 0.0 3.6308367583241514 5.649314082881312E-4 86-87 0.0 9.94279278587111E-4 0.0 3.9253016055802568 5.875286646196565E-4 88-89 0.0 0.0010394737912501615 0.0 4.246702382383541 5.875286646196565E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 54.23056 1 GTATCAA 5305 0.0 41.14226 1 ATCAACG 6475 0.0 33.226086 3 CAACGCA 6730 0.0 32.03627 5 TCAACGC 6775 0.0 31.82348 4 TATCAAC 6950 0.0 31.228575 2 AACGCAG 7120 0.0 30.348171 6 GTGGTAT 1415 0.0 27.892187 1 ACGCAGA 8235 0.0 25.83542 7 CGCAGAG 8345 0.0 25.096516 8 TGGTATC 1530 0.0 24.832436 2 GTACATG 6390 0.0 23.291872 1 TACATGG 6440 0.0 22.197363 2 GCAGAGT 9590 0.0 21.689854 9 GAGTACT 5890 0.0 20.842337 12-13 ACATGGG 6645 0.0 20.654924 3 CAGAGTA 9120 0.0 19.81349 10-11 AGAGTAC 8520 0.0 19.230059 10-11 GTACTTT 6445 0.0 19.047535 14-15 TATAGCG 150 7.735212E-4 18.995955 5 >>END_MODULE