FastQCFastQC Report
Sat 18 Jun 2016
SRR1931108_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931108_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715597
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74290.2735678379376616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58130.214059744505536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32930.12126247009405298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.055.529961
GTATCAA72700.045.5573351
CAACGCA92450.035.131845
ATCAACG92100.034.708193
TCAACGC96300.033.5468374
AACGCAG102550.031.8384326
TATCAAC110100.029.9085852
CGCAGAG108500.028.474388
GTACATG90500.027.7120951
ACGCAGA120100.026.3573597
GACTGAC13200.025.5655467
ACATGGG92850.025.4354883
GCAGAGT117650.025.2498469
TACATGG99400.024.894242
GTGGTAT18000.022.8671781
GTACTAG9400.022.403271
CAGAGTA112900.021.36548610-11
AGAGTAC108400.021.2877910-11
GAGTACT71400.021.03834712-13
CATGGGG60400.020.857214
Produced by FastQC (version 0.11.5)