FastQCFastQC Report
Sat 18 Jun 2016
SRR1931108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715597
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99740.3672857202302109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81920.3016647904678051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52390.19292258755625374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.058.8988381
GTATCAA85550.045.105621
ATCAACG113100.034.0116583
CAACGCA114250.033.8758965
TCAACGC116700.033.1240124
AACGCAG126300.030.5686726
GACTGAC13400.030.1233677
TATCAAC131900.029.2359122
GTGGTAT20250.028.6658361
CGCAGAG136150.027.485148
ACGCAGA149750.025.4012477
GCAGAGT150650.024.2407829
GTACATG103700.022.3449821
ACTGACC20300.021.9897988
TGGTATC24850.021.9774272
CAGACTG21000.021.9352135
AGAGTAC139100.021.38864910-11
CAGAGTA146800.021.22106410-11
ACATGGG103650.021.213733
GAGTACT94250.021.06125812-13
Produced by FastQC (version 0.11.5)