##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931107_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6137247 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543871706646318 33.0 33.0 33.0 27.0 33.0 2 31.496131408757055 33.0 33.0 33.0 27.0 33.0 3 31.497251291988086 33.0 33.0 33.0 27.0 33.0 4 35.190285807300896 37.0 37.0 37.0 33.0 37.0 5 35.19440312570115 37.0 37.0 37.0 33.0 37.0 6 35.27533126823802 37.0 37.0 37.0 33.0 37.0 7 35.24323055598055 37.0 37.0 37.0 33.0 37.0 8 35.25018122946657 37.0 37.0 37.0 33.0 37.0 9 35.254829893598874 37.0 37.0 37.0 33.0 37.0 10-11 35.17685063025816 37.0 37.0 37.0 33.0 37.0 12-13 35.114825833146355 37.0 37.0 37.0 33.0 37.0 14-15 36.91249488573622 40.0 37.0 40.0 33.0 40.0 16-17 36.884195878054115 40.0 37.0 40.0 33.0 40.0 18-19 36.82969236858155 40.0 37.0 40.0 33.0 40.0 20-21 36.75936018217941 40.0 37.0 40.0 33.0 40.0 22-23 36.69933302342239 40.0 37.0 40.0 33.0 40.0 24-25 36.596079805815215 40.0 37.0 40.0 33.0 40.0 26-27 36.43685222380654 40.0 37.0 40.0 33.0 40.0 28-29 36.36133163615543 40.0 37.0 40.0 33.0 40.0 30-31 36.25073147618142 40.0 37.0 40.0 33.0 40.0 32-33 36.113324671469144 40.0 37.0 40.0 33.0 40.0 34-35 36.03013207713491 40.0 37.0 40.0 33.0 40.0 36-37 35.86559869596254 40.0 37.0 40.0 33.0 40.0 38-39 35.72126077050508 40.0 37.0 40.0 27.0 40.0 40-41 35.513043226058855 37.0 37.0 40.0 27.0 40.0 42-43 35.37702645828007 37.0 37.0 40.0 27.0 40.0 44-45 35.16114440236804 37.0 37.0 40.0 27.0 40.0 46-47 35.03470888494466 37.0 37.0 40.0 27.0 40.0 48-49 35.067830657622224 37.0 37.0 40.0 27.0 40.0 50-51 34.2751504868551 37.0 35.0 38.5 24.5 40.0 52-53 34.38454065805075 37.0 35.0 38.5 27.0 40.0 54-55 34.94659592485034 37.0 37.0 40.0 27.0 40.0 56-57 34.937413224528854 37.0 37.0 40.0 27.0 40.0 58-59 34.821945409725245 37.0 37.0 40.0 27.0 40.0 60-61 34.86317953310336 37.0 37.0 40.0 27.0 40.0 62-63 34.73929719628361 37.0 37.0 40.0 27.0 40.0 64-65 34.55918916087295 37.0 35.0 40.0 27.0 40.0 66-67 34.36954509082004 37.0 33.0 40.0 27.0 40.0 68-69 34.19195088612207 37.0 33.0 40.0 22.0 40.0 70-71 33.99065427870184 37.0 33.0 40.0 22.0 40.0 72-73 33.392235883613615 37.0 33.0 40.0 22.0 40.0 74-75 33.193675193454 37.0 33.0 38.5 22.0 40.0 76-77 32.8148045043649 37.0 33.0 37.0 15.0 40.0 78-79 32.53960823150836 37.0 33.0 37.0 15.0 38.5 80-81 32.25885881731662 37.0 33.0 37.0 15.0 37.0 82-83 31.99129243128067 37.0 33.0 37.0 15.0 37.0 84-85 31.62208438083069 37.0 33.0 37.0 15.0 37.0 86-87 31.44851225639118 37.0 33.0 37.0 10.5 37.0 88-89 31.239058571375732 37.0 33.0 37.0 6.0 37.0 90-91 30.9721464689298 37.0 33.0 37.0 2.0 37.0 92-93 30.70342239769721 37.0 33.0 37.0 2.0 37.0 94-95 30.479617082382376 37.0 33.0 37.0 2.0 37.0 96-97 30.21030504393908 33.0 33.0 37.0 2.0 37.0 98-99 29.906127617154727 33.0 33.0 37.0 2.0 37.0 100-101 29.220808043085114 33.0 30.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09343111059640563 1101 2 0.10817072186874555 1101 3 0.1659217321169244 1101 4 0.09001739010861343 1101 5 0.059607202935389125 1101 6 0.12095264563834718 1101 7 0.11073670602831953 1101 8 0.16963783285967793 1101 9 0.19178435932106197 1101 10-11 0.11220212680812125 1101 12-13 0.20702240627184665 1101 14-15 0.27462711033643217 1101 16-17 0.2280520718419794 1101 18-19 0.24233548042293762 1101 20-21 0.3177748576784083 1101 22-23 0.27997522602086633 1101 24-25 0.164153430599292 1101 26-27 0.21551214325906898 1101 28-29 0.23691444928165595 1101 30-31 0.27211454670686663 1101 32-33 0.25389684543903 1101 34-35 0.2587844647336013 1101 36-37 0.16931431385435047 1101 38-39 0.2258867372750757 1101 40-41 0.20642710943457132 1101 42-43 0.14939745574731234 1101 44-45 0.05035135492897069 1101 46-47 0.043395575975822 1101 48-49 -0.009918242314633119 1101 50-51 0.06100607539926273 1101 52-53 0.20168570271673758 1101 54-55 0.27265291850920903 1101 56-57 0.23912689587383795 1101 58-59 0.2605963927230377 1101 60-61 0.21180626925117707 1101 62-63 0.3312229203135715 1101 64-65 0.33552925091488106 1101 66-67 0.330853027886576 1101 68-69 0.270205289162071 1101 70-71 0.12786241979104318 1101 72-73 0.071918974663717 1101 74-75 0.091901347056492 1101 76-77 -0.10576505699690841 1101 78-79 0.010532080475719852 1101 80-81 0.09554037908593926 1101 82-83 0.01697771652001734 1101 84-85 0.14319613439068846 1101 86-87 0.0651006818187767 1101 88-89 0.06686372861310019 1101 90-91 -0.015417195693878227 1101 92-93 -0.022351114993377053 1101 94-95 0.0329740836583845 1101 96-97 0.07489151423164842 1101 98-99 0.168556452573128 1101 100-101 0.16868666505307672 1102 1 0.03938569626386368 1102 2 0.04845372181163654 1102 3 0.0345777937079248 1102 4 0.1293858316482286 1102 5 0.05488596707441218 1102 6 0.1418975566844054 1102 7 0.07088242015557711 1102 8 0.08752921278747294 1102 9 0.06010910249774781 1102 10-11 0.08286797373489208 1102 12-13 0.10123189022537815 1102 14-15 0.24733318389572645 1102 16-17 0.23974058140186827 1102 18-19 0.2615934405694773 1102 20-21 0.22042651832175864 1102 22-23 0.2059775157671666 1102 24-25 0.18255649852121536 1102 26-27 0.20732943403933035 1102 28-29 0.23190349020865142 1102 30-31 0.22752366515867095 1102 32-33 0.20095068870170962 1102 34-35 0.2148824554018418 1102 36-37 0.2259460013784107 1102 38-39 0.22378022634249817 1102 40-41 0.13456791831744397 1102 42-43 0.19350393616642947 1102 44-45 0.19537496059283654 1102 46-47 0.12646485451193712 1102 48-49 0.11834064127631905 1102 50-51 0.09283083785303603 1102 52-53 0.09507132333261126 1102 54-55 0.14951766699276448 1102 56-57 0.21478540410804214 1102 58-59 0.237607647034352 1102 60-61 0.21258059976428711 1102 62-63 0.28518419585029164 1102 64-65 0.26551014495505143 1102 66-67 0.27893438088624833 1102 68-69 0.2550241742704529 1102 70-71 0.29534320816806314 1102 72-73 0.14117050224365357 1102 74-75 0.14194712755052308 1102 76-77 0.0882752215909477 1102 78-79 0.1328318714791621 1102 80-81 0.2146067229697195 1102 82-83 0.2255941497896643 1102 84-85 0.27972390710007033 1102 86-87 0.16432188768173006 1102 88-89 0.2548631674128998 1102 90-91 0.23938052998148507 1102 92-93 0.24774179023615517 1102 94-95 0.1693842867811064 1102 96-97 0.24736022195994067 1102 98-99 0.28786195598434716 1102 100-101 0.2879273273682763 1103 1 0.11094126236932311 1103 2 0.0948021865558033 1103 3 0.057427376330618785 1103 4 0.08297576529884765 1103 5 0.013938390232652864 1103 6 0.07276069447924982 1103 7 0.12639831213640917 1103 8 0.14731419531558743 1103 9 0.07242929540463194 1103 10-11 0.1181388489348123 1103 12-13 0.1361914418681991 1103 14-15 0.1681184208067492 1103 16-17 0.21610363224010598 1103 18-19 0.2214042554087925 1103 20-21 0.24123280485046195 1103 22-23 0.2578979305936002 1103 24-25 0.224261986532305 1103 26-27 0.28431398196699575 1103 28-29 0.2841789391435725 1103 30-31 0.3301612453604861 1103 32-33 0.21594217451416853 1103 34-35 0.2872484045587598 1103 36-37 0.25746744346205475 1103 38-39 0.21571933756042938 1103 40-41 0.33871036839364166 1103 42-43 0.24754587439831255 1103 44-45 0.20438623753069152 1103 46-47 0.13034660454466973 1103 48-49 0.2320246898786067 1103 50-51 0.2286885171946622 1103 52-53 0.34972856325587287 1103 54-55 0.43224762013102236 1103 56-57 0.4345568028895741 1103 58-59 0.45995912157592755 1103 60-61 0.47619358548517 1103 62-63 0.48382566742839117 1103 64-65 0.5656674901263941 1103 66-67 0.5534893076868528 1103 68-69 0.5874920258726632 1103 70-71 0.5955137674122852 1103 72-73 0.40823103157090657 1103 74-75 0.47512668110962863 1103 76-77 0.46689190132593694 1103 78-79 0.496886082301252 1103 80-81 0.5805718489011156 1103 82-83 0.5580702021255917 1103 84-85 0.6004096108090344 1103 86-87 0.46483854351605913 1103 88-89 0.37462565750675125 1103 90-91 0.37066455441723534 1103 92-93 0.24049653810059368 1103 94-95 0.33884673092842377 1103 96-97 0.28107792304185253 1103 98-99 0.28955193179506367 1103 100-101 0.34482219194389074 1104 1 0.04829562326893466 1104 2 0.07990833014937238 1104 3 0.05578139286014405 1104 4 0.1222591744451762 1104 5 0.03322575405743322 1104 6 0.026750913457121328 1104 7 0.07627411220312297 1104 8 0.03560106627492132 1104 9 0.05459827760329716 1104 10-11 0.026246551489734316 1104 12-13 0.08274237931411221 1104 14-15 0.18207597980217116 1104 16-17 0.17184486396710952 1104 18-19 0.19816227310852952 1104 20-21 0.19298580273542143 1104 22-23 0.1738082964931138 1104 24-25 0.21349334433762834 1104 26-27 0.17305226141918695 1104 28-29 0.09050815790806155 1104 30-31 0.13372860641687367 1104 32-33 0.19082774732661534 1104 34-35 0.18154229054431426 1104 36-37 0.2213233677011175 1104 38-39 0.207374330308987 1104 40-41 0.24791737739847974 1104 42-43 0.12715848143821518 1104 44-45 0.17531671207651556 1104 46-47 0.1384074105146169 1104 48-49 0.17750624784679303 1104 50-51 0.07683390254552336 1104 52-53 0.10060886004507807 1104 54-55 0.23014710352791212 1104 56-57 0.25932252087721963 1104 58-59 0.19503175475725243 1104 60-61 0.22531669221434925 1104 62-63 0.1439182809118975 1104 64-65 0.17456668912708295 1104 66-67 0.2439909485125682 1104 68-69 0.18876206599880163 1104 70-71 0.283464313780442 1104 72-73 0.20988563029099083 1104 74-75 0.26110794274681126 1104 76-77 0.1640268195533281 1104 78-79 0.26214610930629334 1104 80-81 0.29879655632147717 1104 82-83 0.2798944825798273 1104 84-85 0.3025675374204262 1104 86-87 0.2736154317610655 1104 88-89 0.29862759598288946 1104 90-91 0.3766237102775065 1104 92-93 0.32434770101235344 1104 94-95 0.20899982088530322 1104 96-97 0.212870425129843 1104 98-99 0.2535626786367473 1104 100-101 0.394737742706333 1105 1 -0.03419176596078444 1105 2 -0.06917790025280723 1105 3 -0.013961620091457405 1105 4 0.010534566401553036 1105 5 -0.05155304621523271 1105 6 -0.04858378320272294 1105 7 -0.08124838268440016 1105 8 -0.0458757571742936 1105 9 -0.04129095211721534 1105 10-11 -0.018644041557301705 1105 12-13 0.016408023485887213 1105 14-15 0.04652178757312697 1105 16-17 -0.013191605017212282 1105 18-19 0.03535586547389613 1105 20-21 0.08761753416009554 1105 22-23 0.10759357256673496 1105 24-25 0.042989595189702356 1105 26-27 0.023043570212642805 1105 28-29 0.031432663702190666 1105 30-31 0.08965191930030159 1105 32-33 0.0638802353975052 1105 34-35 0.12125888904466109 1105 36-37 0.09802769626281105 1105 38-39 0.1300607652101462 1105 40-41 0.19301025401743033 1105 42-43 0.15358026057932506 1105 44-45 0.13358682114263587 1105 46-47 0.08492530417514388 1105 48-49 0.11924738622443698 1105 50-51 0.1821774608277238 1105 52-53 0.25447269780922 1105 54-55 0.30879042322797545 1105 56-57 0.2802022073121222 1105 58-59 0.38404675134893296 1105 60-61 0.3310416410896053 1105 62-63 0.2898218255608356 1105 64-65 0.2945102814924567 1105 66-67 0.35622288443621386 1105 68-69 0.388321370702279 1105 70-71 0.3429622687906715 1105 72-73 0.3056748259283495 1105 74-75 0.3784088970753743 1105 76-77 0.3484695598396286 1105 78-79 0.28434106499818057 1105 80-81 0.32492590192475035 1105 82-83 0.35020242395678736 1105 84-85 0.39568103435294333 1105 86-87 0.39921393210190104 1105 88-89 0.37093894567614427 1105 90-91 0.3202951485869825 1105 92-93 0.3908877487287832 1105 94-95 0.3270381644435858 1105 96-97 0.29374721449589813 1105 98-99 0.3116468640491554 1105 100-101 0.3072133732448492 1106 1 -0.021707894993042487 1106 2 -0.09868582328619624 1106 3 -0.06992483457928955 1106 4 -0.09781291520569368 1106 5 -0.1143597807936132 1106 6 0.0069148019698843655 1106 7 -6.96317036407379E-4 1106 8 0.01189249409905102 1106 9 9.385328856126307E-4 1106 10-11 -0.04161829169877507 1106 12-13 -0.076878620543539 1106 14-15 0.012273343883258292 1106 16-17 -0.020224146047205238 1106 18-19 -0.04294803944969772 1106 20-21 0.018899509259135527 1106 22-23 -0.0014969763862922036 1106 24-25 0.057154281098029 1106 26-27 0.08349221197834567 1106 28-29 0.0649294095991948 1106 30-31 0.056550900624579015 1106 32-33 0.008012663241309781 1106 34-35 0.0375354877996088 1106 36-37 0.11647690395946597 1106 38-39 -0.03914183807225413 1106 40-41 0.11060999934849747 1106 42-43 0.08777055486341823 1106 44-45 0.060662514408058144 1106 46-47 0.050379888215886126 1106 48-49 0.0539195707179303 1106 50-51 0.03815425765851188 1106 52-53 0.1330560311425515 1106 54-55 0.1989886405454655 1106 56-57 0.17366259214517754 1106 58-59 0.20464097885317756 1106 60-61 0.11105097895038085 1106 62-63 0.1396432743440883 1106 64-65 0.22597193967015983 1106 66-67 0.2617298170904334 1106 68-69 0.2821648907928278 1106 70-71 0.31401008995648283 1106 72-73 0.21822561823168485 1106 74-75 0.23358405270638372 1106 76-77 0.20160906182039184 1106 78-79 0.26500886352676645 1106 80-81 0.2449418894742692 1106 82-83 0.3486507827570122 1106 84-85 0.3995307796840102 1106 86-87 0.38688908773291075 1106 88-89 0.4471241465816327 1106 90-91 0.4119103155366126 1106 92-93 0.4296742512754719 1106 94-95 0.46160961322683747 1106 96-97 0.4259932529792003 1106 98-99 0.46914007287767845 1106 100-101 0.4623482062952178 1107 1 -0.041204996390774795 1107 2 -0.021126264824655294 1107 3 -0.024281722994800248 1107 4 0.03091310370401601 1107 5 -0.013933447328270177 1107 6 -0.11648917512796686 1107 7 -0.04481978500497519 1107 8 -0.07390450185850028 1107 9 -0.11334432010167461 1107 10-11 -0.10870103215949456 1107 12-13 -0.051302770864921854 1107 14-15 -0.04775011876382251 1107 16-17 -0.02945592485760784 1107 18-19 0.017437925640855667 1107 20-21 0.033876013185668796 1107 22-23 -0.08416801718492906 1107 24-25 -0.00715798710671578 1107 26-27 -0.03860011383208928 1107 28-29 -0.12197266259590833 1107 30-31 -0.06590115992477052 1107 32-33 -0.060737184668646194 1107 34-35 -0.02072458604328631 1107 36-37 -0.06685707561728549 1107 38-39 -0.07065436053144225 1107 40-41 -0.05495537049036159 1107 42-43 -0.13568921755371832 1107 44-45 -0.2046369017533607 1107 46-47 -0.24054914061930788 1107 48-49 -0.2439858919821063 1107 50-51 -0.3065293696442666 1107 52-53 -0.28007202051912117 1107 54-55 -0.15533042257450802 1107 56-57 -0.10697558971628496 1107 58-59 -0.0932362853497537 1107 60-61 -0.07665319274249072 1107 62-63 -0.11355589796517762 1107 64-65 -0.15196145946412543 1107 66-67 -0.12960896572391079 1107 68-69 -0.06161659853172807 1107 70-71 -0.0867483680550265 1107 72-73 -0.14566831209864262 1107 74-75 -0.03163076796374753 1107 76-77 -0.07901521466553874 1107 78-79 -0.027516970387971185 1107 80-81 -0.004441019375192923 1107 82-83 -0.0055313792668201245 1107 84-85 0.0877849089788647 1107 86-87 0.14977318027746378 1107 88-89 0.06699550313926039 1107 90-91 -0.011933338838861829 1107 92-93 0.02136581614097466 1107 94-95 0.06957755384874176 1107 96-97 0.1473135897780935 1107 98-99 0.17402731766389934 1107 100-101 0.18011216308051559 1108 1 -0.05067236114409468 1108 2 -0.09071624969290681 1108 3 0.004820555088784317 1108 4 0.03362859922867045 1108 5 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-0.6850136032522229 2316 52-53 -0.7995130458709667 2316 54-55 -0.7491956143969531 2316 56-57 -0.7812860348983435 2316 58-59 -0.7707033956234355 2316 60-61 -0.7950286117028682 2316 62-63 -0.8161138005414585 2316 64-65 -0.8258728754740332 2316 66-67 -0.8266887851698996 2316 68-69 -0.8091568773870819 2316 70-71 -0.7155330280935175 2316 72-73 -0.41378034749905623 2316 74-75 -0.658593748427279 2316 76-77 -0.7377929328117503 2316 78-79 -0.7320052019036396 2316 80-81 -0.6984105178283357 2316 82-83 -0.6644214737504441 2316 84-85 -0.622274579991565 2316 86-87 -0.6359618468516572 2316 88-89 -0.6599500901851165 2316 90-91 -0.6442833346581693 2316 92-93 -0.635959840924361 2316 94-95 -0.6594472852918649 2316 96-97 -0.6415489348280303 2316 98-99 -0.5537578978556077 2316 100-101 -0.6106321587951307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128088.0 3 20001.0 4 15055.0 5 12977.0 6 13016.0 7 13857.0 8 13510.0 9 14494.0 10 14874.0 11 15234.0 12 15961.0 13 16995.0 14 17990.0 15 20394.0 16 24288.0 17 30047.0 18 26358.0 19 20328.0 20 20866.0 21 22179.0 22 24454.0 23 28412.0 24 33708.0 25 38802.0 26 43807.0 27 51962.0 28 62826.0 29 76851.0 30 96046.0 31 121693.0 32 158384.0 33 213050.0 34 297256.0 35 440321.0 36 707265.0 37 1416030.0 38 1848535.0 39 1333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.748082479649185 25.587321822915676 13.674767887691225 22.989827809743918 2 13.292239535917858 22.036976596284482 45.11809238918517 19.55269147861249 3 16.132330472275736 32.5192867609403 30.60896582030916 20.739416946474808 4 10.106757309655183 19.32555138010207 45.34199766635909 25.225693643883663 5 11.120695765525388 39.30760738945353 37.74842454732468 11.823272297696397 6 23.596540924619784 43.6655718761197 21.592531635112618 11.145355564147899 7 22.383554059336376 33.18481397277965 29.148280980055063 15.283350987828909 8 21.575553338492 39.091012631559394 22.626447982295645 16.706986047652965 9 25.068764545406108 15.708101694456813 25.083249867570917 34.13988389256616 10-11 21.441665373333382 28.720691743889866 31.8734838976951 17.964158985081653 12-13 21.950467009372122 26.40810376907025 31.407946532343594 20.233482689214032 14-15 20.106414380272334 27.94744996636411 28.344448750714207 23.601686902649345 16-17 18.403533219748176 29.95487010489898 30.560903849337585 21.08069282601526 18-19 18.03107101157543 31.187876369366723 31.657616787598318 19.123435831459524 20-21 18.651049892565837 29.246688295256813 30.97238061300124 21.12988119917611 22-23 19.003178460961408 30.665011527155418 29.94033807014774 20.39147194173544 24-25 18.918923983255358 30.439701256907313 30.754606320603223 19.88676843923411 26-27 19.28384990859908 29.653857829088516 30.307742217316658 20.754550044995746 28-29 18.944267682236028 31.22452949995332 29.365976308269815 20.46522650954084 30-31 20.476803361507205 30.157878605830916 29.079357568629717 20.28596046403216 32-33 20.24294443420641 30.538317913553097 29.161519814991966 20.057217837248526 34-35 20.68605842326372 29.295953055172784 30.272579871724243 19.745408649839252 36-37 20.635824173281602 30.00385188994349 30.431828798808326 18.928495137966582 38-39 21.350843464504525 28.91726534714995 31.251495988347873 18.480395199997655 40-41 20.058081558591887 29.32744761807929 31.174811380765792 19.43965944256303 42-43 20.84459381292521 29.3249855065285 30.067012141928156 19.763408538618137 44-45 19.74325928739368 30.951792620205605 30.046282480286013 19.258665612114704 46-47 21.081072250608837 29.424066482965923 29.24573731472377 20.24912395170147 48-49 20.92428388409174 28.240184410152846 30.363851900736428 20.471679805018983 50-51 20.880773664284742 28.95909080161365 28.760333742989037 21.399801791112573 52-53 19.24460468417503 29.707752721174284 29.775017106346162 21.272625488304524 54-55 20.201259239608287 30.401255397222936 28.859747620684622 20.537737742484154 56-57 20.710613452028795 29.159002207560363 29.698298449215816 20.43208589119503 58-59 20.474294319783922 28.36235867660028 30.94968458317897 20.213662420436822 60-61 20.28050883027522 30.74747366466124 28.823333461067364 20.148684043996177 62-63 20.853961361699472 29.59354855629878 27.840977376988185 21.711512705013565 64-65 19.412828809232884 29.51460432108428 28.699388794644737 22.373178075038098 66-67 20.371889167300704 27.691649284761915 29.897649485061585 22.038812062875795 68-69 19.256797150792764 29.057782887115646 29.0383615228963 22.647058439195288 70-71 19.78551683612216 28.667615380003475 29.88519708237159 21.661670701502775 72-73 20.671005736313607 28.345752975373777 30.259662262417862 20.723579025894747 74-75 20.61161373983599 29.545273001937826 28.838961099182946 21.004152159043233 76-77 19.846553019244837 29.442565628269936 30.255714899577185 20.455166452908045 78-79 19.632559436112818 29.022016652096077 29.80958904882512 21.535834862965984 80-81 19.811040550368343 29.377549957883144 30.564022608570852 20.24738688317766 82-83 20.94594776797946 27.983592192873942 30.752389216536034 20.318070822610565 84-85 22.10772738812139 28.192815423285943 29.204835583288375 20.494621605304296 86-87 20.645746421707226 29.521075706001294 29.3067412313315 20.52643664095998 88-89 21.355741967421217 28.298187099678067 29.622797795406036 20.72327313749468 90-91 21.182256954753033 27.394195289115476 30.83003290596482 20.59351485016667 92-93 20.786697423012775 28.79092544068526 29.52802760231809 20.89434953398387 94-95 19.72549929719337 28.26833654294892 30.26862731444766 21.73753684541005 96-97 20.46204714713188 28.279442438260865 29.194003224127446 22.064507190479805 98-99 19.511553451820483 29.17423048933601 29.85573750800311 21.458478550840393 100-101 20.486144458619826 27.500992435459054 29.478486895976037 22.53437620994508 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 50.0 1 34.5 2 33.0 3 49.5 4 40.0 5 37.0 6 69.0 7 103.0 8 164.5 9 223.0 10 243.5 11 331.5 12 529.5 13 831.5 14 1132.5 15 1434.5 16 1908.0 17 2279.5 18 2512.0 19 2780.0 20 3039.5 21 3425.0 22 3906.0 23 4404.5 24 4961.0 25 5716.5 26 7432.0 27 11267.0 28 22766.5 29 52524.0 30 92703.0 31 132780.0 32 184387.0 33 248048.0 34 336434.5 35 406417.5 36 449612.0 37 509651.0 38 501837.0 39 396134.0 40 297366.0 41 229314.0 42 182117.5 43 156361.0 44 133567.5 45 125527.5 46 125505.0 47 127964.5 48 130272.5 49 125000.5 50 117583.0 51 116025.0 52 113642.0 53 109354.5 54 104520.5 55 93315.5 56 82816.0 57 68844.0 58 54879.0 59 51001.5 60 44121.5 61 33807.5 62 26060.0 63 21541.0 64 17226.0 65 11520.0 66 8172.5 67 6387.5 68 5231.5 69 4312.5 70 3542.5 71 2928.0 72 2481.0 73 2127.5 74 1741.5 75 1463.5 76 1261.5 77 1060.0 78 881.0 79 730.5 80 620.5 81 512.0 82 429.0 83 363.0 84 298.5 85 248.0 86 203.0 87 169.5 88 147.0 89 117.5 90 87.0 91 78.0 92 64.5 93 43.0 94 25.5 95 18.0 96 13.0 97 10.5 98 9.0 99 5.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.9105481659773507E-4 2 0.003747608659061628 3 9.776370414943377E-5 4 9.124612387280486E-4 5 0.0019878619843718202 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.332277811207533E-5 12-13 6.517580276628918E-5 14-15 0.003266937113660246 16-17 0.004806715454013827 18-19 0.003918695141323137 20-21 0.0 22-23 0.0 24-25 8.146975345786147E-6 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.4440926037358447E-4 42-43 1.6293950691572295E-5 44-45 7.087868550833949E-4 46-47 0.7583856409885409 48-49 0.5487558183661175 50-51 0.42392786211798217 52-53 0.3556399147696027 54-55 0.3463197749740234 56-57 0.3125342682150482 58-59 0.31335711272497263 60-61 0.28938871125766974 62-63 0.28414205913498347 64-65 0.28040259745126767 66-67 0.26751408245423397 68-69 0.24641341630864785 70-71 0.26027956834717586 72-73 0.8384378207362356 74-75 0.5585158948303695 76-77 0.6521979643315643 78-79 0.3708747586662228 80-81 0.3217077624544034 82-83 0.3006641251362378 84-85 0.29170245225587305 86-87 0.2640679118829664 88-89 0.26709043973625307 90-91 0.2783006778120548 92-93 0.2327264977277271 94-95 0.19817517528624806 96-97 0.15295946211713493 98-99 0.1620270456769949 100-101 0.157147007444869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6137247.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.363814467787382 #Duplication Level Percentage of deduplicated Percentage of total 1 76.72510235370707 20.227663634751302 2 11.595743104748164 6.114160396594109 3 4.4453484276916155 3.5158902357699633 4 2.098764888241014 2.2132579250037048 5 1.2234604715043178 1.6127542439705753 6 0.7489148240228094 1.1846550883627789 7 0.508319996421212 0.9380877853140626 8 0.35080569761334746 0.7398861060896812 9 0.27399832108643524 0.6501276811447213 >10 1.5625547817611634 7.8091108505480635 >50 0.1824290522807751 3.390096027760032 >100 0.21096877784064294 12.08124834777587 >500 0.038102075722584734 6.902239908696746 >1k 0.029946239227499137 15.599116677031317 >5k 0.003299689561450009 5.982648132045383 >10k+ 0.0022412985700415154 11.039056959141815 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 47675 0.7768140992207092 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 40977 0.667677217488558 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 32039 0.5220418862072849 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 31792 0.5180172803864664 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 31669 0.516013124451403 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 27350 0.4456395514145023 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 26825 0.4370852273014268 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 26727 0.4354884201336528 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 23840 0.38844778448708356 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 23341 0.38031710309198896 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 23014 0.37498898121584484 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 22572 0.3677870550101699 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 21364 0.34810396257475057 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 20266 0.33021320471540416 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 18259 0.29751124567741855 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 16279 0.2652492233081054 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 15975 0.2602958622978674 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 15794 0.25734665722269284 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 15261 0.2486619815040848 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 13495 0.21988686458276813 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 12538 0.20429355377093344 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 12442 0.20272933450454253 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 12096 0.1970916275652585 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 11981 0.19521782323572767 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 11819 0.19257820322369298 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 11412 0.18594656529222303 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 11037 0.1798363337828834 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 10912 0.1777995899464369 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 10881 0.17729447747499816 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 10645 0.1734491051117871 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 10554 0.171966355598854 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 10476 0.17069542744491137 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 10396 0.1693919113895856 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 10306 0.16792545582734408 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 10289 0.16764845866558736 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 10255 0.1670944643420739 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 9617 0.15669892380085076 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 9491 0.15464588601371265 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 9458 0.15410818564089077 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 9313 0.1517455627906128 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 9177 0.14952958549655895 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 9087 0.14806312993431747 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 8955 0.1459123284430299 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 8860 0.14436440312733054 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 8738 0.14237654114295872 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 8714 0.141985486326361 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 8454 0.1377490591465522 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 8101 0.13199729455242717 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 8062 0.13136183047545585 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 7887 0.1285103891044307 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 7742 0.1261477662541527 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 7480 0.12187875117296078 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 7183 0.11703944781756381 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6979 0.11371548187648305 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6942 0.11311260570089489 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 6923 0.11280302063775502 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 6832 0.11132027112482194 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6772 0.11034263408332759 No Hit ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA 6762 0.11017969457641187 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 6727 0.10960940630220684 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 6592 0.10740972295884457 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 6450 0.10509598196064131 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 6434 0.10483527874957616 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 6416 0.10454198763712787 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 6407 0.10439534208090369 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 6300 0.10265188935690546 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6275 0.10224454058961616 No Hit ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT 6222 0.10138096120296282 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 6205 0.10110396404120608 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 6169 0.10051738181630951 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 6144 0.10011003304902018 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.6293950691572295E-5 0.0 4 0.0 0.0 0.0 1.6293950691572295E-5 0.0 5 0.0 0.0 0.0 1.6293950691572295E-5 0.0 6 1.6293950691572295E-5 0.0 0.0 1.6293950691572295E-5 0.0 7 1.6293950691572295E-5 0.0 0.0 1.6293950691572295E-5 0.0 8 1.6293950691572295E-5 0.0 0.0 1.6293950691572295E-5 0.0 9 1.6293950691572295E-5 0.0 0.0 1.6293950691572295E-5 0.0 10-11 1.6293950691572295E-5 0.0 0.0 1.6293950691572295E-5 0.0 12-13 1.6293950691572295E-5 0.0 0.0 1.6293950691572295E-5 0.0 14-15 1.6293950691572295E-5 0.0 0.0 2.4440926037358442E-5 0.0 16-17 1.6293950691572295E-5 0.0 0.0 4.8881852074716884E-5 0.0 18-19 1.6293950691572295E-5 0.0 0.0 8.146975345786148E-5 0.0 20-21 1.6293950691572295E-5 0.0 0.0 9.776370414943378E-5 0.0 22-23 1.6293950691572295E-5 0.0 0.0 1.9552740829886756E-4 0.0 24-25 1.6293950691572295E-5 0.0 0.0 4.3178969332666585E-4 0.0 26-27 1.6293950691572295E-5 0.0 0.0 0.0011161356223727023 0.0 28-29 1.6293950691572295E-5 0.0 0.0 0.0031610264341650255 0.0 30-31 1.6293950691572295E-5 0.0 0.0 0.014306088707200475 0.0 32-33 1.6293950691572295E-5 0.0 0.0 0.05382706610960908 0.0 34-35 1.6293950691572295E-5 0.0 0.0 0.11600478194864897 0.0 36-37 1.6293950691572295E-5 0.0 0.0 0.21260346862363533 0.0 38-39 1.6293950691572295E-5 0.0 0.0 0.34950524233422575 0.0 40-41 1.6293950691572295E-5 0.0 0.0 0.48876963889509417 0.0 42-43 2.4440926037358442E-5 0.0 0.0 0.6500308688895852 0.0 44-45 3.258790138314459E-5 0.0 0.0 0.8275534616742654 0.0 46-47 3.258790138314459E-5 0.0 0.0 1.045941282793409 0.0 48-49 4.888185207471689E-5 0.0 0.0 1.2716695286991055 0.0 50-51 4.888185207471689E-5 0.0 0.0 1.4951492094093655 0.0 52-53 4.888185207471689E-5 0.0 0.0 1.7216595649482578 0.0 54-55 4.888185207471689E-5 0.0 0.0 1.9573352677511595 0.0 56-57 4.888185207471689E-5 0.0 0.0 2.2471394747514646 0.0 58-59 4.888185207471689E-5 0.0 0.0 2.58307185615961 1.6293950691572295E-5 60-61 4.888185207471689E-5 0.0 0.0 2.927240829642346 1.6293950691572295E-5 62-63 4.888185207471689E-5 0.0 0.0 3.258040616582647 1.6293950691572295E-5 64-65 5.7028827420503034E-5 0.0 0.0 3.6062912247136216 1.6293950691572295E-5 66-67 6.517580276628918E-5 0.0 0.0 3.9869016189180586 1.6293950691572295E-5 68-69 6.517580276628918E-5 0.0 0.0 4.4029676498273576 1.6293950691572295E-5 70-71 6.517580276628918E-5 0.0 0.0 4.825029854591154 1.6293950691572295E-5 72-73 8.146975345786148E-5 0.0 0.0 5.308300285127843 1.6293950691572295E-5 74-75 8.146975345786148E-5 0.0 0.0 5.748196218923566 1.6293950691572295E-5 76-77 8.146975345786148E-5 0.0 0.0 6.241291901727273 1.6293950691572295E-5 78-79 8.146975345786148E-5 0.0 0.0 6.7853876501956005 1.6293950691572295E-5 80-81 8.146975345786148E-5 0.0 0.0 7.417845493264325 2.4440926037358442E-5 82-83 8.146975345786148E-5 0.0 0.0 8.087103223969965 3.258790138314459E-5 84-85 8.146975345786148E-5 0.0 0.0 8.711911057188997 3.258790138314459E-5 86-87 8.146975345786148E-5 0.0 0.0 9.388419595952389 3.258790138314459E-5 88-89 8.146975345786148E-5 0.0 0.0 10.096220667018942 3.258790138314459E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3225 0.0 46.39411 2 ACCTGGG 3335 0.0 45.14708 3 CCTGGGG 3415 0.0 38.959557 4 GTACCTG 4060 0.0 37.31895 1 CTGGGGG 3455 0.0 35.218952 5 GTACACG 520 0.0 33.69022 1 TATTCGC 1370 0.0 33.522697 9 CTGTGCG 3125 0.0 29.998737 9 GTATCAA 10425 0.0 28.74969 1 TATTCTC 6690 0.0 28.096727 5 TCTAACG 1340 0.0 27.914368 2 TCTCTGC 6800 0.0 27.293415 8 GTACTAG 3060 0.0 27.23308 1 TGGGGGG 11850 0.0 27.128897 6 CTAACGC 1415 0.0 27.103601 3 GTACCCG 315 0.0 27.056238 1 TAACGCC 1455 0.0 27.009312 4 GGTATCA 7110 0.0 26.571056 1 CAACGCA 10880 0.0 26.06689 5 TAGTACT 3950 0.0 25.891373 4 >>END_MODULE