FastQCFastQC Report
Sat 18 Jun 2016
SRR1931107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137247
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA522350.851114514374279No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC434400.7078092180419006No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC351100.5720806087811033No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC332880.5423930306210586No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG316360.5154754240785812No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC312660.5094466623226994No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA282120.45968493691063766No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC279040.4546664000976333No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA252220.41096602434283647No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA245380.39982096206980106No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC245350.39977208021772626No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA230970.37634137912324533No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG223370.3639579765976504No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT179810.2929815273851615No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT174780.2847856701873006No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC169830.2767201645949723No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA161400.2629843641619769No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA160780.2619741392190994No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA150360.24499584259848103No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC140410.2287833616603666No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA133980.2183063513656856No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA130300.21231017751118703No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA126720.20647694316360413No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG126480.20608588834700642No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG120600.19650504534036187No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC117540.19151909642874076No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT115320.1879018393752117No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC112500.18330694528018834No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA111950.18241077799215186No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT111730.18205231107693728No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG109760.17884240279069752No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT108790.177261889573615No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA107800.17564878845514936No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG107280.1748015030191876No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA105410.17175453423986356No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC104620.17046731213522937No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA103810.169147502129212No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG98690.160804999375127No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG94670.15425483119711494No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT94460.1539126582325919No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC93940.15306537279663016No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC92420.15058869229151117No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT91580.1492200004334191No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT90500.1474602537587293No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG90360.14723213844904726No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA89550.1459123284430299No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG88550.14428293337387268No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT86500.14094267348210035No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC83760.13647813099260955No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA83760.13647813099260955No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG81190.13229058566487545No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA80640.131394418376839No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT77540.1263432936624516No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT76160.12409472846701461No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT75730.12339408858727699No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA74910.12205798463056808No Hit
ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT72680.11842443362634744No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT70380.11467682496728582No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT70140.1142857701506881No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT70030.1141065366930808No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC69040.11249343557461512No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA68820.11213496865940054No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC68190.1111084497658315No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA68130.11101068606168205No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC66240.1079311293809749No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA65470.10667649517772382No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC65420.10659502542426597No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT64720.1054544488758559No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA64100.1044442239329784No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA63730.10384134775739025No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG63680.10375987800393238No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG61600.10037073626008533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG34200.047.6368373
TACCTGG35100.046.9566652
CTGGGGG28550.044.0874145
CCTGGGG31850.043.9933934
GTACCTG43450.038.6239131
TGGGGGG102250.030.5659186
TATTCGC13250.030.1119069
GTATCAA105450.028.0423971
TATTCTC67450.027.1115065
CTGTGCG34350.027.1021799
GTACACG4300.026.5346971
GGGGGGA107900.026.4562287
TATATCC113500.026.1552584
GGTATCA70250.025.5809571
CATGGGG108550.025.2476444
AGGGCTA116650.024.878915
ATATATC122600.024.7562733
CAACGCA111050.024.6364885
ATGGGGG98650.024.6036035
TCTCTGC69000.024.3685158
Produced by FastQC (version 0.11.5)