FastQCFastQC Report
Sat 18 Jun 2016
SRR1931106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286989
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA120360.36617098505653656No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC116990.35591844085879204No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA110650.33663027165591364No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC96840.2946161365310319No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC88590.2695171781834378No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC79400.24155845973320872No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG76140.23164056831343213No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC75150.22862869331172084No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA66000.20079166678075283No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT65790.20015278420463228No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA64610.19656287258643093No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT59410.18074292308249282No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC58680.17852204555597842No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC57120.173776060704797No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC54990.16729596600414542No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA44880.13653833341091193No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG44730.13608198871368296No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT44310.1348042235614418No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA43880.13349603542938537No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC42340.12881089653783448No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA39230.11934934981528687No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC39180.11919723491621056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38400.11682424249061985No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC36860.11213910359906894No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA36670.1115610669825789No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT35760.10879257581938973No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA35320.10745396470751803No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT34800.10587196975712422No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC33660.10240375005818395No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT32880.10003075763259323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35050.049.7274132
ACCTGGG36950.047.9394723
GTACCTG38800.046.01991
CCTGGGG37900.042.1140254
CTGGGGG36250.040.764555
GTACCCG3300.040.18651
GTACTAG26250.032.477251
GTACACG4550.031.2281271
TATTCTC22050.029.8567335
GTATCAA101750.029.6976681
TGGGGGG93950.029.3901126
GCATCGT29050.027.3900368
AGGGCTA46750.026.8472825
TAGGCAT35850.026.8190235
CAACGCA106750.026.576335
GCTATTG48450.025.9048798
TATTCGC5700.025.7594249
GGGGGGA93950.025.7100477
ATCAACG109900.025.5560113
TATCAAC117450.025.485912
Produced by FastQC (version 0.11.5)