##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931106_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286989 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.847587868410876 33.0 33.0 33.0 27.0 33.0 2 30.761011369371786 33.0 33.0 33.0 27.0 33.0 3 30.786239016924 33.0 33.0 33.0 27.0 33.0 4 34.36284666605212 37.0 37.0 37.0 27.0 37.0 5 34.32399469544924 37.0 37.0 37.0 27.0 37.0 6 34.421982549987234 37.0 37.0 37.0 33.0 37.0 7 34.37953610431918 37.0 37.0 37.0 33.0 37.0 8 34.37846126044231 37.0 37.0 37.0 33.0 37.0 9 34.39382851600659 37.0 37.0 37.0 33.0 37.0 10-11 34.28726138116069 37.0 37.0 37.0 30.0 37.0 12-13 34.174540742302455 37.0 37.0 37.0 27.0 37.0 14-15 35.70790455946157 40.0 37.0 40.0 27.0 40.0 16-17 35.64377246166629 40.0 37.0 40.0 27.0 40.0 18-19 35.56466662955063 40.0 37.0 40.0 27.0 40.0 20-21 35.47303428761094 40.0 37.0 40.0 27.0 40.0 22-23 35.35591129145854 40.0 37.0 40.0 27.0 40.0 24-25 35.215285326479645 38.5 37.0 40.0 27.0 40.0 26-27 35.01706987762965 37.0 37.0 40.0 27.0 40.0 28-29 34.88545519927204 37.0 37.0 40.0 27.0 40.0 30-31 34.7297841580851 37.0 37.0 40.0 24.5 40.0 32-33 34.563485153129506 37.0 37.0 40.0 22.0 40.0 34-35 34.42384930402871 37.0 37.0 40.0 22.0 40.0 36-37 34.191157621762656 37.0 33.0 40.0 22.0 40.0 38-39 33.96792657352975 37.0 33.0 40.0 22.0 40.0 40-41 33.71189696710272 37.0 33.0 40.0 15.0 40.0 42-43 33.49488255056527 37.0 33.0 40.0 15.0 40.0 44-45 33.174636574688876 37.0 33.0 40.0 15.0 40.0 46-47 32.99684559333785 37.0 33.0 40.0 6.0 40.0 48-49 32.94678594908593 37.0 33.0 40.0 6.0 40.0 50-51 32.12191796200109 37.0 33.0 38.5 6.0 40.0 52-53 32.239032439719146 37.0 33.0 38.5 6.0 40.0 54-55 32.690541556421394 37.0 33.0 40.0 6.0 40.0 56-57 32.60206149153527 37.0 33.0 40.0 6.0 40.0 58-59 32.40747611263683 37.0 33.0 40.0 2.0 40.0 60-61 32.40750197216967 37.0 33.0 40.0 2.0 40.0 62-63 32.21371839090426 37.0 33.0 40.0 2.0 40.0 64-65 31.973898756582393 37.0 33.0 40.0 2.0 40.0 66-67 31.719590634468204 37.0 33.0 38.5 2.0 40.0 68-69 31.468212093195323 37.0 33.0 37.0 2.0 40.0 70-71 31.247873966113062 37.0 33.0 37.0 2.0 40.0 72-73 30.662118887528983 37.0 33.0 37.0 2.0 40.0 74-75 30.479612800651296 37.0 33.0 37.0 2.0 40.0 76-77 30.146061943012285 37.0 33.0 37.0 2.0 37.0 78-79 29.900511684097513 37.0 27.0 37.0 2.0 37.0 80-81 29.669134426674383 37.0 27.0 37.0 2.0 37.0 82-83 29.40786583100826 33.0 27.0 37.0 2.0 37.0 84-85 29.033899717948554 33.0 27.0 37.0 2.0 37.0 86-87 28.869849427546 33.0 27.0 37.0 2.0 37.0 88-89 28.68778721194382 33.0 27.0 37.0 2.0 37.0 90-91 28.395900473046915 33.0 27.0 37.0 2.0 37.0 92-93 28.102697027583602 33.0 27.0 37.0 2.0 37.0 94-95 27.85040853498445 33.0 27.0 37.0 2.0 37.0 96-97 27.54984668339322 33.0 27.0 37.0 2.0 37.0 98-99 27.20133517331515 33.0 27.0 37.0 2.0 37.0 100-101 26.48696360103426 33.0 24.5 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17803369533742242 1101 2 0.14759144270148994 1101 3 0.1794523961148471 1101 4 0.27542440901296317 1101 5 0.22333194910348908 1101 6 0.2035067151003247 1101 7 0.2622162317749428 1101 8 0.30477505000826 1101 9 0.18931850313379073 1101 10-11 0.2910668699782235 1101 12-13 0.4160974841639984 1101 14-15 0.5203081336587516 1101 16-17 0.4163213923206541 1101 18-19 0.505322860861213 1101 20-21 0.5432671014743207 1101 22-23 0.474149951587691 1101 24-25 0.4511951725904524 1101 26-27 0.3780323196924513 1101 28-29 0.49299824466957887 1101 30-31 0.46757574410277414 1101 32-33 0.4208543798167028 1101 34-35 0.5815893863781412 1101 36-37 0.5010250183515197 1101 38-39 0.5246398011799016 1101 40-41 0.4516875915132843 1101 42-43 0.40947177968136117 1101 44-45 0.4107544618036556 1101 46-47 0.24559837657970007 1101 48-49 0.22360197305602725 1101 50-51 0.37157124728236113 1101 52-53 0.4789428431131242 1101 54-55 0.6339206427463253 1101 56-57 0.5665255283708959 1101 58-59 0.6100795925212523 1101 60-61 0.4957311571127221 1101 62-63 0.4827112584919391 1101 64-65 0.6778085731606325 1101 66-67 0.6601739034525309 1101 68-69 0.6326354315047631 1101 70-71 0.48239797853505806 1101 72-73 0.4761148727948239 1101 74-75 0.5479849114154938 1101 76-77 0.46115601360565606 1101 78-79 0.44083148810224415 1101 80-81 0.6832661766985773 1101 82-83 0.4976046268708245 1101 84-85 0.6212885223847415 1101 86-87 0.6733396067136859 1101 88-89 0.679619058205688 1101 90-91 0.609892183864293 1101 92-93 0.5814279543015672 1101 94-95 0.4456536165821561 1101 96-97 0.3876384714624912 1101 98-99 0.47158698364204454 1101 100-101 0.39808560898547185 1102 1 0.059756478368342414 1102 2 0.018475355300520135 1102 3 0.05836084231980365 1102 4 0.14022762904874497 1102 5 0.12902770683412967 1102 6 0.1033725469429001 1102 7 0.07852245462186147 1102 8 0.04498876071615854 1102 9 0.13223314662982943 1102 10-11 0.13820301871321305 1102 12-13 0.14828985574590092 1102 14-15 0.2736663306942759 1102 16-17 0.2972215967673719 1102 18-19 0.20959308191922332 1102 20-21 0.21840845721160917 1102 22-23 0.1948973001693446 1102 24-25 0.1815219678881732 1102 26-27 0.14674318731319858 1102 28-29 0.15982676498646953 1102 30-31 0.2143868545039993 1102 32-33 0.19187325919314446 1102 34-35 0.10994678479368503 1102 36-37 0.14240471423703127 1102 38-39 0.23386146614284087 1102 40-41 0.17444426797635515 1102 42-43 0.1874482363642116 1102 44-45 0.27554889701960406 1102 46-47 0.12469609572102058 1102 48-49 0.405019683252803 1102 50-51 0.2350080285243763 1102 52-53 0.2847647828013464 1102 54-55 0.32345233198731904 1102 56-57 0.42037407807700333 1102 58-59 0.3242399234693707 1102 60-61 0.5628527709639926 1102 62-63 0.4987619229437712 1102 64-65 0.45233259564977146 1102 66-67 0.47494438006893347 1102 68-69 0.4394588406537494 1102 70-71 0.487940401228645 1102 72-73 0.23443888889500286 1102 74-75 0.39145140132093914 1102 76-77 0.2379017968921353 1102 78-79 0.2752959335405265 1102 80-81 0.47439430728891807 1102 82-83 0.26300840913507173 1102 84-85 0.3797491025023021 1102 86-87 0.33506414670857154 1102 88-89 0.24773655117783022 1102 90-91 0.31659260553564295 1102 92-93 0.21038751218554097 1102 94-95 0.07669038504625192 1102 96-97 0.16718741089515277 1102 98-99 0.031304591615469946 1102 100-101 0.04259589521087648 1103 1 0.3259611355442722 1103 2 0.2952360774784779 1103 3 0.2498507477631975 1103 4 0.35081225645963343 1103 5 0.24341113462385522 1103 6 0.34482862848106066 1103 7 0.2843356564678956 1103 8 0.26532369254219645 1103 9 0.27650337708337247 1103 10-11 0.3061161589245742 1103 12-13 0.34719396121635526 1103 14-15 0.5221968699224888 1103 16-17 0.475427727162284 1103 18-19 0.4947075570473771 1103 20-21 0.4461335365098762 1103 22-23 0.36148228836856333 1103 24-25 0.4112186050403537 1103 26-27 0.4982953287422234 1103 28-29 0.3868250061433969 1103 30-31 0.5199452495972849 1103 32-33 0.4383984166622099 1103 34-35 0.4286724503729644 1103 36-37 0.44795859947628003 1103 38-39 0.7306534566420879 1103 40-41 0.5760512792960881 1103 42-43 0.5098374067014007 1103 44-45 0.46370977854574136 1103 46-47 0.632688308706399 1103 48-49 0.6003392064109008 1103 50-51 0.71674537462561 1103 52-53 0.8259653059444148 1103 54-55 0.8375809530242861 1103 56-57 0.8435637475047031 1103 58-59 0.7790053358955191 1103 60-61 0.759881528799859 1103 62-63 0.8309457445940751 1103 64-65 0.8243421866060103 1103 66-67 0.8396442492831682 1103 68-69 0.8181557934957091 1103 70-71 0.7802728977335072 1103 72-73 0.6841081662660571 1103 74-75 0.8171853800644904 1103 76-77 0.7209936024873613 1103 78-79 0.8122459192593219 1103 80-81 0.836920426860182 1103 82-83 0.754149552533395 1103 84-85 0.7348753032509379 1103 86-87 0.7292441800814942 1103 88-89 0.7290898080440869 1103 90-91 0.8320358490226631 1103 92-93 0.702408077119923 1103 94-95 0.6923508531690992 1103 96-97 0.6648685942808505 1103 98-99 0.7213445789878783 1103 100-101 0.7908821506790744 1104 1 0.04847163189130299 1104 2 0.11277750898704042 1104 3 0.06696589977957146 1104 4 0.20902902170976745 1104 5 0.1427262698971532 1104 6 0.17992160626121745 1104 7 0.20749893002682995 1104 8 0.16526162559949142 1104 9 0.15191615164395245 1104 10-11 0.09875627018291766 1104 12-13 0.14360957500510096 1104 14-15 0.15631604331729676 1104 16-17 0.17272626449457817 1104 18-19 0.07770298837715472 1104 20-21 0.17254794625669945 1104 22-23 0.15271023478990742 1104 24-25 0.09957535739467005 1104 26-27 0.14933661217795446 1104 28-29 0.09847758815455876 1104 30-31 0.09649530662711214 1104 32-33 0.16861265301888295 1104 34-35 0.045174503832022594 1104 36-37 0.08413964400494933 1104 38-39 0.07253802145288546 1104 40-41 0.18400990525316274 1104 42-43 0.13996732971771308 1104 44-45 0.08675217156488912 1104 46-47 0.01765700057564601 1104 48-49 0.058764439156867354 1104 50-51 0.02115970856237226 1104 52-53 0.1224020512771844 1104 54-55 0.22633761351466575 1104 56-57 0.28019146321216226 1104 58-59 0.14660329048368226 1104 60-61 0.19097977314861936 1104 62-63 0.21584384595778516 1104 64-65 0.2740078622691371 1104 66-67 0.3153939528841221 1104 68-69 0.3066556727874641 1104 70-71 0.24819080518160064 1104 72-73 0.1244702362695449 1104 74-75 0.10471069484341555 1104 76-77 0.0518914873667633 1104 78-79 0.20392471249309096 1104 80-81 0.16502874522913658 1104 82-83 0.14574047503019116 1104 84-85 0.2038976759311062 1104 86-87 0.19025703880338796 1104 88-89 0.19020974339068175 1104 90-91 0.25400719201386934 1104 92-93 0.18635692114845526 1104 94-95 0.051063880314973886 1104 96-97 0.0024256519834473522 1104 98-99 0.08810918047938898 1104 100-101 0.2256316420524378 1105 1 0.011951687491965401 1105 2 -0.06684456444539677 1105 3 -0.02972755901559765 1105 4 -0.09067892530552513 1105 5 -0.1606873018167363 1105 6 -0.08741996213441894 1105 7 -0.20003171282324672 1105 8 -0.05778926526082273 1105 9 -0.06590732238542785 1105 10-11 -0.048940082116132544 1105 12-13 -0.0638665463043111 1105 14-15 -0.09652441490515429 1105 16-17 -0.16340435259457564 1105 18-19 -0.08461398255209929 1105 20-21 -0.05999878758771615 1105 22-23 -0.14359359001740302 1105 24-25 -0.11056847635229161 1105 26-27 -0.06469728413892284 1105 28-29 -0.11836288808519413 1105 30-31 -0.06895087872428007 1105 32-33 -0.017980203998014588 1105 34-35 -0.1282395988175935 1105 36-37 -0.07979979558759709 1105 38-39 0.027367322741469025 1105 40-41 0.0032947175779227678 1105 42-43 0.07781195875656266 1105 44-45 0.024200803054093 1105 46-47 -0.19298599900803737 1105 48-49 -0.11866440970546677 1105 50-51 0.04611373566194743 1105 52-53 0.023996276843213593 1105 54-55 0.03282644354699471 1105 56-57 -0.014872329577997334 1105 58-59 0.037057832801750124 1105 60-61 0.20304672510219035 1105 62-63 0.2245576608670703 1105 64-65 0.18454236504112487 1105 66-67 0.15077220337998654 1105 68-69 0.0805417427973758 1105 70-71 0.1628149026867014 1105 72-73 0.12090525203660718 1105 74-75 0.1975947377138887 1105 76-77 0.017215250823205963 1105 78-79 0.041776653877786174 1105 80-81 0.21820842729638912 1105 82-83 0.30085028097222377 1105 84-85 0.34763041093629354 1105 86-87 0.2797549288609744 1105 88-89 0.2791591116730032 1105 90-91 0.28952573796300385 1105 92-93 0.28634093557457163 1105 94-95 0.2212743512875477 1105 96-97 0.2917700977823827 1105 98-99 0.16070242199342388 1105 100-101 0.2659949765128111 1106 1 0.03587232409996233 1106 2 0.07398597208515056 1106 3 0.12720382946410425 1106 4 0.07885250265844945 1106 5 0.013579201850745903 1106 6 -0.0062042261323540515 1106 7 0.21935013048492635 1106 8 0.22815357365852407 1106 9 0.1921493679211821 1106 10-11 0.11694778254391736 1106 12-13 0.13525359978750373 1106 14-15 0.13558674092106315 1106 16-17 0.19680992552657983 1106 18-19 0.20768639406713874 1106 20-21 0.20193438527747531 1106 22-23 0.2904050280329926 1106 24-25 0.23942677794162393 1106 26-27 0.266387850031677 1106 28-29 0.23873403062275145 1106 30-31 0.265704924704778 1106 32-33 0.2401254619953903 1106 34-35 0.32845393487312435 1106 36-37 0.332997665269815 1106 38-39 0.17444719487316007 1106 40-41 0.30780769423664367 1106 42-43 0.23432097939468832 1106 44-45 0.24485772267322403 1106 46-47 0.07021465703837038 1106 48-49 0.178481332826685 1106 50-51 0.2030168397333867 1106 52-53 0.31587846184222457 1106 54-55 0.3427178238261135 1106 56-57 0.399371622494165 1106 58-59 0.3866189499029957 1106 60-61 0.3880911069455024 1106 62-63 0.3864767864422518 1106 64-65 0.4289612248567565 1106 66-67 0.4586255876379113 1106 68-69 0.4149932910365308 1106 70-71 0.5248788672116014 1106 72-73 0.208023317610877 1106 74-75 0.2814521235511833 1106 76-77 0.1923940690284951 1106 78-79 0.18509659799235223 1106 80-81 0.3311146597372776 1106 82-83 0.2816347272052724 1106 84-85 0.4176842462261199 1106 86-87 0.41588171134818097 1106 88-89 0.400925197718351 1106 90-91 0.39188871993691166 1106 92-93 0.4328344282623853 1106 94-95 0.2671822238444612 1106 96-97 0.4225644150840857 1106 98-99 0.5139782879898718 1106 100-101 0.5041624126166262 1107 1 -0.07913504114825187 1107 2 -0.058999229321234026 1107 3 -0.0838017509210367 1107 4 -0.09055071511276225 1107 5 -0.1387866377337943 1107 6 -0.058301081834422064 1107 7 -0.05457169394619399 1107 8 -0.017157039108624872 1107 9 -0.09272194631866171 1107 10-11 -0.04218673806981599 1107 12-13 -0.053010387911996304 1107 14-15 0.06328518149550888 1107 16-17 0.07193312767128646 1107 18-19 0.0945151439820151 1107 20-21 0.11396912917829383 1107 22-23 -9.344436045353177E-4 1107 24-25 0.15658144457005818 1107 26-27 0.190172763279449 1107 28-29 0.13675105850317237 1107 30-31 0.21822461016746786 1107 32-33 0.06935474521876017 1107 34-35 0.056559345732118516 1107 36-37 0.23749503636005898 1107 38-39 0.1417762261369404 1107 40-41 0.14896160104385814 1107 42-43 0.13483260430106014 1107 44-45 0.2160093356805035 1107 46-47 -0.021513595767054028 1107 48-49 4.923468072348669E-5 1107 50-51 -0.07070364999290035 1107 52-53 0.11093442697646339 1107 54-55 0.20891644181165958 1107 56-57 0.25456976738347237 1107 58-59 0.2684604770002963 1107 60-61 0.11598380370156747 1107 62-63 0.20282656225915474 1107 64-65 0.156908130556495 1107 66-67 0.10059325581107714 1107 68-69 0.15770787781205442 1107 70-71 0.08430987938665879 1107 72-73 0.14322696403595359 1107 74-75 0.28840529419197836 1107 76-77 0.20414818084407216 1107 78-79 0.2235806471226809 1107 80-81 0.3783560965162067 1107 82-83 0.465236028936733 1107 84-85 0.4228987882008646 1107 86-87 0.4763977088106621 1107 88-89 0.5369610634306987 1107 90-91 0.5158833904361373 1107 92-93 0.5110981650775166 1107 94-95 0.558552249613868 1107 96-97 0.6659456374644073 1107 98-99 0.6669645287743542 1107 100-101 0.6789824926845789 1108 1 -0.14325206378246946 1108 2 -0.049744441026486896 1108 3 -0.14189790875754582 1108 4 -0.0014150289829188978 1108 5 -0.10063013973619661 1108 6 -0.07571666422153811 1108 7 -0.12475082129017068 1108 8 -0.043209460557143586 1108 9 -0.08062420188206687 1108 10-11 -0.04707228757215631 1108 12-13 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17642.0 4 13385.0 5 11248.0 6 11460.0 7 12237.0 8 12554.0 9 12834.0 10 13115.0 11 13594.0 12 13869.0 13 15248.0 14 15513.0 15 17498.0 16 20840.0 17 26026.0 18 22596.0 19 17478.0 20 17542.0 21 18283.0 22 19869.0 23 22344.0 24 25921.0 25 29597.0 26 33799.0 27 39734.0 28 47699.0 29 57242.0 30 69901.0 31 87376.0 32 111521.0 33 146308.0 34 200698.0 35 290105.0 36 445377.0 37 699217.0 38 544177.0 39 345.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62691886215102 22.299294122801093 12.624346891700391 23.449440123347493 2 15.018569332352866 20.895252658227232 42.06940278459361 22.016775224826286 3 18.03956823588826 29.375962129371036 29.141795812569594 23.442673822171113 4 11.815187060611702 17.60951469992297 41.53107008429684 29.04422815516849 5 12.558862203270301 38.30447039381012 35.501963239683185 13.634704163236396 6 27.110738733838176 39.84181267415254 19.89726768175981 13.150180910249471 7 25.187975986533573 32.102024071270094 25.677025387063967 17.03297455513237 8 24.73488654814482 35.69640786750427 21.145187890802188 18.423517693548717 9 26.251046170218395 15.010606971912594 22.994327026953847 35.744019830915164 10-11 24.54435489268595 26.53487022161322 28.833676910289906 20.087097975410924 12-13 24.828935205220713 24.654961209898858 28.779226437110843 21.73687714776959 14-15 22.222683653630114 25.75112793358187 26.62545425006469 25.40073416272333 16-17 20.878532617872583 27.925072554644547 28.088056192693696 23.10833863478917 18-19 20.462734360144513 28.633337136337705 29.824133865753943 21.079794637763833 20-21 21.710313254822765 27.504370642002552 28.93108692044405 21.854229182730638 22-23 22.18381321020545 27.799788803674126 28.168302358176433 21.848095627943994 24-25 21.762302824864946 27.740144551746294 29.177888943346026 21.319663680042737 26-27 21.80383019231278 27.708200422940266 28.294877165697844 22.193092219049106 28-29 21.111281479798077 28.44998872828598 28.470874103929155 21.967855687986788 30-31 22.256280748125413 27.785109715913258 28.271679643588705 21.686929892372625 32-33 22.38299245905599 28.111685192740225 27.63016547971411 21.875156868489672 34-35 22.347397572672133 27.460161868506404 28.757184766970624 21.435255791850842 36-37 22.353223573306753 27.50927976941815 28.40115984568248 21.736336811592615 38-39 22.759492045759814 27.126862913140265 28.993312724806806 21.12033231629312 40-41 22.01503962981245 26.801937643416114 29.209373642847204 21.973649083924236 42-43 22.308638696387483 27.06925700086006 28.32434182164893 22.29776248110353 44-45 21.948481428184618 28.215849247179015 28.2610276333523 21.574641691284068 46-47 22.485479121563472 27.445379675919 27.606693824611817 22.462447377905708 48-49 22.977784764790837 26.800702113252008 28.059006752594694 22.162506369362465 50-51 22.622144876960856 27.16203984991598 27.409165902225446 22.806649370897716 52-53 21.674856094815016 27.809631207094032 27.637732586678908 22.87778011141204 54-55 22.03784499950932 27.98470486086469 27.299280678531783 22.678169461094203 56-57 22.859266177968678 27.16878358323837 27.39850632160909 22.573443917183862 58-59 22.11754905505792 27.144164885412327 28.453550837075113 22.28473522245464 60-61 22.175359118971624 28.47105121663826 27.199020740622103 22.154568923768014 62-63 22.631212972152483 27.553134098480825 26.62361243996881 23.19204048939789 64-65 21.482164871361682 27.77302517341583 27.277420641886795 23.46738931333569 66-67 22.36582896855376 26.638107644263425 27.606019690079048 23.390043697103767 68-69 21.547874288463962 27.293801300197078 27.384672994548616 23.773651416790344 70-71 21.81801186184341 26.989612728836992 27.7781810274551 23.4141943818645 72-73 22.748167720004336 26.806028983693626 28.026250551896187 22.419552744405856 74-75 22.660850753214522 27.56829535998811 27.242167228106634 22.528686658690738 76-77 22.320018503718426 27.348076404682196 27.558830206187572 22.773074885411802 78-79 21.92164834138213 27.51332798850108 27.465029104504495 23.099994565612295 80-81 21.668063007921994 27.71652202544601 28.30911898063376 22.30629598599824 82-83 22.75599759339624 26.50908517327059 28.918968723914222 21.815948509418952 84-85 23.3528014627562 26.997000995905484 27.314214205556787 22.33598333578153 86-87 21.90566787555238 27.940799267531762 27.77569197295211 22.377840883963746 88-89 22.799115929959424 26.68913390812424 27.794602707567435 22.717147454348897 90-91 22.545312290291484 26.419200453885477 28.324528117717396 22.71095913810564 92-93 22.700036527441622 26.945726563743435 27.851686390875336 22.50255051793961 94-95 21.36412641099209 26.960239993245466 28.520595386616325 23.155038209146113 96-97 21.88144389575696 27.04292822810233 27.660110243935733 23.415517632204978 98-99 21.315017453368306 27.734157899355495 27.693434388985853 23.25739025829035 100-101 21.94234038793182 26.784998263264697 27.41535700574646 23.85730434305702 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 113.0 1 77.5 2 43.5 3 59.5 4 56.5 5 45.0 6 62.5 7 124.5 8 214.5 9 267.5 10 275.0 11 293.5 12 401.5 13 550.0 14 699.5 15 907.5 16 1256.0 17 1585.0 18 1827.0 19 2168.0 20 2519.0 21 2902.5 22 3306.0 23 3666.5 24 4172.5 25 4845.5 26 5860.5 27 7344.0 28 10831.0 29 20236.0 30 34280.0 31 47833.0 32 64149.5 33 83425.5 34 106063.5 35 122221.5 36 132241.5 37 151879.0 38 153193.5 39 129458.0 40 107904.5 41 92241.5 42 85886.5 43 86746.5 44 90261.0 45 101591.5 46 114879.5 47 124955.0 48 130963.0 49 127919.5 50 120979.0 51 120170.5 52 118616.5 53 113860.5 54 108618.5 55 96473.0 56 85164.5 57 70545.0 58 55505.0 59 51060.5 60 43490.0 61 32726.5 62 24957.0 63 20421.5 64 16049.0 65 10088.5 66 6830.0 67 5242.5 68 4141.5 69 3285.0 70 2597.5 71 2080.5 72 1748.0 73 1400.0 74 1113.5 75 900.0 76 736.0 77 612.5 78 493.0 79 430.5 80 353.0 81 285.0 82 249.5 83 209.0 84 161.5 85 132.5 86 105.0 87 83.5 88 69.0 89 54.5 90 40.5 91 34.0 92 31.0 93 29.5 94 24.0 95 14.5 96 12.0 97 9.0 98 5.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.5634469722898377E-4 2 0.003589911618201339 3 2.7380681833739023E-4 4 0.0010039583339037643 5 0.0022817234861449184 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.042297981526558E-5 12-13 1.0648042935342954E-4 14-15 0.003513854168663175 16-17 0.004745984851181431 18-19 0.003468219698940276 20-21 1.521148990763279E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.9774936879922629E-4 42-43 0.0 44-45 5.780366164900461E-4 46-47 0.7332242365277157 48-49 0.5303029611598943 50-51 0.4063293184126871 52-53 0.3413762565070951 54-55 0.3320820361735315 56-57 0.29746068514375923 58-59 0.29826689410886376 60-61 0.2738524528071132 62-63 0.267646164924799 64-65 0.26375202350844495 66-67 0.25196311883002953 68-69 0.23243156578862906 70-71 0.24557429306882378 72-73 0.8104225478089523 74-75 0.5344861208844933 76-77 0.6272762093210534 78-79 0.3514158398461327 80-81 0.303393166207736 82-83 0.2841810544543958 84-85 0.27586036947492065 86-87 0.24983351024296094 88-89 0.25219129117864403 90-91 0.26251989282592675 92-93 0.2209621023982739 94-95 0.18953516424910458 96-97 0.14642580185087326 98-99 0.15518762003766975 100-101 0.1511717867020547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3286989.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.017076137864464 #Duplication Level Percentage of deduplicated Percentage of total 1 84.9284320770817 30.588738043897024 2 8.857791818847385 6.380635247055586 3 2.4438900701216726 2.640653241844297 4 1.034555567405863 1.490466665604342 5 0.5308623977552431 0.9560055699339941 6 0.3413732280753172 0.7377159328210363 7 0.20833594347297296 0.5252556076823925 8 0.15969517033762937 0.46014024871197057 9 0.12844920045424416 0.4163728169347495 >10 0.9162939071372734 6.833144466729581 >50 0.16724413053914577 4.279756069439712 >100 0.2190666797883517 17.156077927759714 >500 0.0415129928286513 10.286448254621853 >1k 0.021223411466590632 13.607819540831207 >5k 0.0010187237503963501 2.5767182905698007 >10k+ 2.5468093759908753E-4 1.0640520755628091 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 12036 0.36617098505653656 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 11699 0.35591844085879204 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11065 0.33663027165591364 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9684 0.2946161365310319 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8859 0.2695171781834378 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 7940 0.24155845973320872 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 7614 0.23164056831343213 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 7515 0.22862869331172084 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 6600 0.20079166678075283 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 6579 0.20015278420463228 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6461 0.19656287258643093 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5941 0.18074292308249282 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 5868 0.17852204555597842 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5712 0.173776060704797 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 5499 0.16729596600414542 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4488 0.13653833341091193 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 4473 0.13608198871368296 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 4431 0.1348042235614418 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 4388 0.13349603542938537 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4234 0.12881089653783448 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 3923 0.11934934981528687 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3918 0.11919723491621056 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3840 0.11682424249061985 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 3686 0.11213910359906894 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 3667 0.1115610669825789 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 3576 0.10879257581938973 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3532 0.10745396470751803 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 3480 0.10587196975712422 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 3366 0.10240375005818395 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3288 0.10003075763259323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.0422979815265582E-5 7 0.0 0.0 0.0 0.0 3.0422979815265582E-5 8 0.0 0.0 0.0 0.0 3.0422979815265582E-5 9 0.0 0.0 0.0 0.0 3.0422979815265582E-5 10-11 0.0 0.0 0.0 0.0 3.0422979815265582E-5 12-13 0.0 0.0 0.0 0.0 3.0422979815265582E-5 14-15 0.0 0.0 0.0 0.0 3.0422979815265582E-5 16-17 0.0 0.0 0.0 0.0 3.0422979815265582E-5 18-19 0.0 0.0 0.0 3.0422979815265582E-5 3.0422979815265582E-5 20-21 0.0 0.0 0.0 6.0845959630531164E-5 3.0422979815265582E-5 22-23 0.0 0.0 0.0 1.5211489907632791E-4 3.0422979815265582E-5 24-25 0.0 0.0 0.0 2.1296085870685909E-4 3.0422979815265582E-5 26-27 0.0 0.0 0.0 4.1071022750608537E-4 3.0422979815265582E-5 28-29 0.0 0.0 0.0 0.0016428409100243415 3.0422979815265582E-5 30-31 0.0 0.0 0.0 0.006160653412591281 3.0422979815265582E-5 32-33 0.0 0.0 0.0 0.019120842813894416 3.0422979815265582E-5 34-35 0.0 0.0 0.0 0.04174032830654438 3.0422979815265582E-5 36-37 0.0 0.0 0.0 0.0766811206243769 3.0422979815265582E-5 38-39 0.0 0.0 0.0 0.12438435297471333 3.0422979815265582E-5 40-41 0.0 0.0 0.0 0.17668145527715487 3.0422979815265582E-5 42-43 0.0 0.0 0.0 0.23106253169694208 3.0422979815265582E-5 44-45 0.0 0.0 0.0 0.29835816304830953 3.0422979815265582E-5 46-47 0.0 0.0 0.0 0.38218868392927385 3.0422979815265582E-5 48-49 0.0 0.0 0.0 0.47370100721359276 3.0422979815265582E-5 50-51 0.0 0.0 0.0 0.5687119731766672 3.0422979815265582E-5 52-53 0.0 0.0 0.0 0.6638750540388179 3.0422979815265582E-5 54-55 0.0 0.0 0.0 0.7699751961445567 3.0422979815265582E-5 56-57 0.0 0.0 0.0 0.8969302909136598 3.0422979815265582E-5 58-59 0.0 0.0 0.0 1.0457595081699391 3.0422979815265582E-5 60-61 0.0 0.0 0.0 1.2033201206331996 3.0422979815265582E-5 62-63 0.0 0.0 0.0 1.363040764663344 3.0422979815265582E-5 64-65 0.0 0.0 0.0 1.5329378954416946 3.0422979815265582E-5 66-67 0.0 0.0 0.0 1.7258956449200165 3.0422979815265582E-5 68-69 0.0 0.0 0.0 1.9450171570394668 3.0422979815265582E-5 70-71 0.0 0.0 0.0 2.181540613613249 3.0422979815265582E-5 72-73 0.0 0.0 0.0 2.4377477381275083 3.0422979815265582E-5 74-75 0.0 0.0 0.0 2.688828590542895 3.0422979815265582E-5 76-77 0.0 0.0 0.0 2.9721121670927406 3.0422979815265582E-5 78-79 0.0 0.0 0.0 3.2882981963127955 3.0422979815265582E-5 80-81 0.0 0.0 0.0 3.6377517539608437 3.0422979815265582E-5 82-83 0.0 0.0 0.0 4.00707151742826 3.0422979815265582E-5 84-85 0.0 0.0 0.0 4.37817102521487 3.0422979815265582E-5 86-87 0.0 0.0 0.0 4.782690176328549 3.0422979815265582E-5 88-89 0.0 0.0 0.0 5.2184080932427825 3.0422979815265582E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3505 0.0 49.727413 2 ACCTGGG 3695 0.0 47.939472 3 GTACCTG 3880 0.0 46.0199 1 CCTGGGG 3790 0.0 42.114025 4 CTGGGGG 3625 0.0 40.76455 5 GTACCCG 330 0.0 40.1865 1 GTACTAG 2625 0.0 32.47725 1 GTACACG 455 0.0 31.228127 1 TATTCTC 2205 0.0 29.856733 5 GTATCAA 10175 0.0 29.697668 1 TGGGGGG 9395 0.0 29.390112 6 GCATCGT 2905 0.0 27.390036 8 AGGGCTA 4675 0.0 26.847282 5 TAGGCAT 3585 0.0 26.819023 5 CAACGCA 10675 0.0 26.57633 5 GCTATTG 4845 0.0 25.904879 8 TATTCGC 570 0.0 25.759424 9 GGGGGGA 9395 0.0 25.710047 7 ATCAACG 10990 0.0 25.556011 3 TATCAAC 11745 0.0 25.48591 2 >>END_MODULE