##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931106_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286989 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63931732050214 33.0 33.0 33.0 33.0 33.0 2 32.60261351650401 33.0 33.0 33.0 33.0 33.0 3 32.65322883648226 33.0 33.0 33.0 33.0 33.0 4 36.60216812407951 37.0 37.0 37.0 37.0 37.0 5 36.5495698342769 37.0 37.0 37.0 37.0 37.0 6 36.551837258962536 37.0 37.0 37.0 37.0 37.0 7 36.49416867534391 37.0 37.0 37.0 37.0 37.0 8 36.50404275767275 37.0 37.0 37.0 37.0 37.0 9 36.49217870823419 37.0 37.0 37.0 37.0 37.0 10-11 36.47546432312369 37.0 37.0 37.0 37.0 37.0 12-13 36.38785465969007 37.0 37.0 37.0 37.0 37.0 14-15 38.34829870133426 40.0 37.0 40.0 37.0 40.0 16-17 38.34539832655357 40.0 37.0 40.0 37.0 40.0 18-19 38.30269906592325 40.0 37.0 40.0 37.0 40.0 20-21 38.221668067644885 40.0 37.0 40.0 37.0 40.0 22-23 38.088702761098375 40.0 37.0 40.0 33.0 40.0 24-25 38.23883545092484 40.0 37.0 40.0 37.0 40.0 26-27 38.175439285011294 40.0 37.0 40.0 37.0 40.0 28-29 38.108550104670265 40.0 37.0 40.0 35.0 40.0 30-31 37.982353302673054 40.0 37.0 40.0 33.0 40.0 32-33 37.85900941560802 40.0 37.0 40.0 33.0 40.0 34-35 37.78412005637986 40.0 37.0 40.0 33.0 40.0 36-37 37.72729525410642 40.0 37.0 40.0 33.0 40.0 38-39 37.58105594512181 40.0 37.0 40.0 33.0 40.0 40-41 37.39449690887314 40.0 37.0 40.0 33.0 40.0 42-43 37.22047122761896 40.0 37.0 40.0 33.0 40.0 44-45 36.977405005006105 40.0 37.0 40.0 33.0 40.0 46-47 36.71314081063247 40.0 37.0 40.0 33.0 40.0 48-49 36.45190735350803 37.0 37.0 40.0 33.0 40.0 50-51 36.20337138335419 37.0 37.0 40.0 33.0 40.0 52-53 35.96372120502989 37.0 37.0 40.0 33.0 40.0 54-55 35.724290832734766 37.0 37.0 40.0 33.0 40.0 56-57 35.40405672182048 37.0 37.0 40.0 27.0 40.0 58-59 35.11851545593855 37.0 33.0 40.0 27.0 40.0 60-61 34.88195397064 37.0 33.0 40.0 27.0 40.0 62-63 34.56997133242612 37.0 33.0 40.0 27.0 40.0 64-65 34.21907253112195 37.0 33.0 38.5 27.0 40.0 66-67 33.87331354014266 37.0 33.0 37.0 27.0 40.0 68-69 33.57973786952132 37.0 33.0 37.0 27.0 40.0 70-71 33.3223211273296 37.0 33.0 37.0 27.0 40.0 72-73 32.99337341865154 37.0 33.0 37.0 24.5 40.0 74-75 32.59640403420882 37.0 33.0 37.0 22.0 38.5 76-77 30.938929214548637 33.0 30.0 37.0 22.0 37.0 78-79 31.74623021251364 33.0 33.0 37.0 22.0 37.0 80-81 31.814348633354115 33.0 33.0 37.0 22.0 37.0 82-83 31.58639837249227 33.0 33.0 37.0 22.0 37.0 84-85 31.291328629332195 33.0 33.0 37.0 22.0 37.0 86-87 30.947844820898396 33.0 33.0 37.0 15.0 37.0 88-89 30.617543289618553 33.0 33.0 37.0 15.0 37.0 90-91 30.212297637746886 33.0 33.0 37.0 15.0 37.0 92-93 29.79771410856562 33.0 27.0 37.0 6.0 37.0 94-95 29.330480874745852 33.0 27.0 37.0 2.0 37.0 96-97 28.841375343817703 33.0 27.0 37.0 2.0 37.0 98-99 28.17870154113689 33.0 27.0 37.0 2.0 37.0 100-101 26.440670473798363 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07275847005519864 1101 2 0.2078215144587645 1101 3 0.10155639779089398 1101 4 0.1395999423894949 1101 5 0.07284854761322634 1101 6 0.07727571672300115 1101 7 0.035185477622270867 1101 8 0.14739593676009122 1101 9 0.12149202529654701 1101 10-11 0.1634653296815074 1101 12-13 0.1294781559699345 1101 14-15 0.18889920513826297 1101 16-17 0.19022996774154421 1101 18-19 0.1904066406889129 1101 20-21 0.15883790176842894 1101 22-23 0.1287697783371371 1101 24-25 0.13963061880439653 1101 26-27 0.13531155520791316 1101 28-29 0.12047357100021117 1101 30-31 0.04692956303232876 1101 32-33 0.10509178000481967 1101 34-35 0.08416800134467195 1101 36-37 -0.07795209810475257 1101 38-39 0.006213106598814022 1101 40-41 -0.004062734956455927 1101 42-43 -0.04623884555589797 1101 44-45 -0.05657592939201095 1101 46-47 -0.21331051736833473 1101 48-49 -0.31449770308154257 1101 50-51 -0.10972662880623574 1101 52-53 -0.2556649927544896 1101 54-55 -0.26529125859764235 1101 56-57 -0.2663777755495431 1101 58-59 -0.36774453766422255 1101 60-61 -0.4339019644123141 1101 62-63 -0.6137396316923116 1101 64-65 -0.5296507412937714 1101 66-67 -0.5846129854808595 1101 68-69 -0.6743928846591842 1101 70-71 -0.7789339399803197 1101 72-73 -0.9958969901120653 1101 74-75 -1.0803370914593415 1101 76-77 -1.1593212144058818 1101 78-79 -0.9752169416063694 1101 80-81 -1.043262967914817 1101 82-83 -1.2144602141246885 1101 84-85 -1.1539301958213457 1101 86-87 -1.368719103774346 1101 88-89 -1.625444144880582 1101 90-91 -1.6317402755198671 1101 92-93 -1.8056000777721053 1101 94-95 -1.7362522377883955 1101 96-97 -2.1314290183771867 1101 98-99 -2.4729431707575564 1101 100-101 -4.029820482473372 1102 1 0.09799645881062702 1102 2 0.21112300947537932 1102 3 0.12294823270017474 1102 4 0.16021871315361835 1102 5 0.09631679448263952 1102 6 0.10287947343140758 1102 7 0.04618087757115319 1102 8 0.08385613354005272 1102 9 0.10269251085750142 1102 10-11 0.11548503962271894 1102 12-13 0.08866787613349203 1102 14-15 0.13344862374291466 1102 16-17 0.12409385095135406 1102 18-19 0.1633382149286291 1102 20-21 0.02116220537180169 1102 22-23 0.14834555140127748 1102 24-25 0.20257305427590921 1102 26-27 0.1648764670402656 1102 28-29 0.15830819277378794 1102 30-31 0.11732312296076941 1102 32-33 0.18305625738789644 1102 34-35 0.18765637343769725 1102 36-37 0.20901362512551458 1102 38-39 0.21362190419656457 1102 40-41 0.11411864761446822 1102 42-43 0.14156542865826083 1102 44-45 0.10210267398135642 1102 46-47 0.08916440596414077 1102 48-49 0.10762087601378312 1102 50-51 0.08497372897551259 1102 52-53 0.04523026024831722 1102 54-55 0.011069589856226969 1102 56-57 -0.04981279982759901 1102 58-59 -0.04502938944035151 1102 60-61 -0.0020250477243521914 1102 62-63 -0.08252317373166562 1102 64-65 -0.20543658335868287 1102 66-67 -0.20991646107503215 1102 68-69 -0.31216760993390835 1102 70-71 -0.40524687123510716 1102 72-73 -0.33972525487055805 1102 74-75 -0.4662414487299813 1102 76-77 -0.66218107257097 1102 78-79 -0.5101956983147815 1102 80-81 -0.5148129295810584 1102 82-83 -0.7023723020367747 1102 84-85 -0.7800714237794324 1102 86-87 -0.7438133409325403 1102 88-89 -0.7957594400505279 1102 90-91 -1.0056892916200475 1102 92-93 -1.0691654368316499 1102 94-95 -1.184064019030405 1102 96-97 -1.3433013030692358 1102 98-99 -1.4268556100624359 1102 100-101 -2.1633345701299014 1103 1 0.04235688634479118 1103 2 0.19402464955896193 1103 3 0.06776114892211638 1103 4 0.11752091847223767 1103 5 0.005359051814906479 1103 6 0.1345236158826637 1103 7 0.09877629856602965 1103 8 0.06136248491781515 1103 9 0.12298685399726139 1103 10-11 0.08903700065759068 1103 12-13 0.11918605836165597 1103 14-15 0.16192158716798843 1103 16-17 0.1214375469270692 1103 18-19 0.13762302724353503 1103 20-21 0.15972671883371703 1103 22-23 0.15236383134294584 1103 24-25 0.18360686314829167 1103 26-27 0.1201612643223271 1103 28-29 0.2508165735858583 1103 30-31 0.12870073303879792 1103 32-33 0.15429992480120092 1103 34-35 0.14040588111196683 1103 36-37 0.07937862264508766 1103 38-39 0.1495045577946783 1103 40-41 0.06706283065441454 1103 42-43 0.13313049833550394 1103 44-45 0.12317722186849522 1103 46-47 0.03148788276094194 1103 48-49 0.05912867054482973 1103 50-51 0.07090523932563286 1103 52-53 0.031736025345054486 1103 54-55 -0.03096675956726358 1103 56-57 -0.006580587443529851 1103 58-59 0.010214819155429211 1103 60-61 0.054432311605786765 1103 62-63 -0.11988054959793715 1103 64-65 -0.18672895719551974 1103 66-67 -0.07923968231343537 1103 68-69 -0.2087739447755439 1103 70-71 -0.19211267301199086 1103 72-73 -0.24783622734541666 1103 74-75 -0.40857319359250255 1103 76-77 -0.4100787289501575 1103 78-79 -0.3136239390207223 1103 80-81 -0.4914691734739627 1103 82-83 -0.41242401502966786 1103 84-85 -0.389981828012683 1103 86-87 -0.3359338742979432 1103 88-89 -0.2966020311119948 1103 90-91 -0.3913960609109459 1103 92-93 -0.475505539956977 1103 94-95 -0.5805072474845794 1103 96-97 -0.6375497358949609 1103 98-99 -0.5756116419922037 1103 100-101 -1.2926982297261205 1104 1 0.08092646425917138 1104 2 0.2039647859724525 1104 3 0.13044034897611567 1104 4 0.15308799283074137 1104 5 0.1420664592876335 1104 6 0.08922893675477184 1104 7 0.14226157023458086 1104 8 0.11119502376590162 1104 9 0.09073748272403748 1104 10-11 0.06852632877178877 1104 12-13 0.11595946922394518 1104 14-15 0.13772510779471503 1104 16-17 0.17494525147787954 1104 18-19 0.22042903480467402 1104 20-21 0.1820542981033597 1104 22-23 0.15843390536720392 1104 24-25 0.14958036449615264 1104 26-27 0.258582353866764 1104 28-29 0.18488782854417707 1104 30-31 0.1196925635940147 1104 32-33 0.14974605172870525 1104 34-35 0.15995306607612036 1104 36-37 0.12431497767773436 1104 38-39 0.12612861070116566 1104 40-41 0.004871328393186047 1104 42-43 0.039139366894872296 1104 44-45 0.062284372073534655 1104 46-47 -0.018780158459456686 1104 48-49 -0.025230682864375353 1104 50-51 -6.964891434506626E-4 1104 52-53 0.0463167400392166 1104 54-55 -0.02189276308249788 1104 56-57 0.06507850531289705 1104 58-59 0.062470644417729204 1104 60-61 0.0012322559847248726 1104 62-63 -0.07038317629446311 1104 64-65 0.09840276681618576 1104 66-67 -0.04833163199583623 1104 68-69 -0.03735911645185297 1104 70-71 -0.050357816354804186 1104 72-73 -0.07054835004093718 1104 74-75 -0.21320766044821227 1104 76-77 -0.2131717079465183 1104 78-79 -0.1712551020583355 1104 80-81 -0.12368838288868034 1104 82-83 -0.09677717593365642 1104 84-85 -0.016209560584833582 1104 86-87 -0.03855137835144973 1104 88-89 -0.09110187026902139 1104 90-91 -0.19241911142212942 1104 92-93 -0.10987910268259782 1104 94-95 -0.11179389196363232 1104 96-97 -0.0672871493493794 1104 98-99 -0.05674390964707854 1104 100-101 -0.6924411647465725 1105 1 0.09254255211006068 1105 2 0.153082167619786 1105 3 0.13881811223781426 1105 4 0.1314681750126212 1105 5 0.1018584283193178 1105 6 0.11603448240719416 1105 7 0.10781975043991565 1105 8 0.10775304603223645 1105 9 0.1391459789223859 1105 10-11 0.12664567775640734 1105 12-13 0.12975565200690653 1105 14-15 0.16943208149173472 1105 16-17 0.23623847482126337 1105 18-19 0.22109577124052038 1105 20-21 0.169255967809967 1105 22-23 0.10551216797683338 1105 24-25 0.1294652798646112 1105 26-27 0.19080572485244573 1105 28-29 0.23326920293551723 1105 30-31 0.26811195643355035 1105 32-33 0.20914817593163093 1105 34-35 0.17327907364645512 1105 36-37 0.2127583757123901 1105 38-39 0.22703907922032585 1105 40-41 0.2761108658925764 1105 42-43 0.30129156565880777 1105 44-45 0.1763095759950204 1105 46-47 0.21114381039436125 1105 48-49 0.2040009132165892 1105 50-51 0.26546144717646314 1105 52-53 0.17289426125555707 1105 54-55 0.0022216380411705927 1105 56-57 0.12041307724069128 1105 58-59 0.18458298658414662 1105 60-61 0.11760440666159866 1105 62-63 0.004303236197742422 1105 64-65 0.29099026195725486 1105 66-67 0.42050200954856365 1105 68-69 0.17162998000671337 1105 70-71 0.28502029038589427 1105 72-73 0.1620532503565144 1105 74-75 0.06995493008879095 1105 76-77 0.12438174980594852 1105 78-79 0.40904538072899754 1105 80-81 0.3749374136107484 1105 82-83 0.2842282917459684 1105 84-85 0.3520617874398937 1105 86-87 0.31859030136818944 1105 88-89 0.28470675251053734 1105 90-91 0.3151400141171443 1105 92-93 0.3189994211397398 1105 94-95 0.19274068249425724 1105 96-97 0.3946774393460224 1105 98-99 0.3592523308679567 1105 100-101 -0.561408431504784 1106 1 0.09508587091520582 1106 2 0.15421126601155777 1106 3 0.14332957027058768 1106 4 0.1267505635075068 1106 5 0.11167140953391907 1106 6 0.10467060444397447 1106 7 0.07205838732126324 1106 8 0.10233800556085271 1106 9 0.11759212085316761 1106 10-11 0.1065329957111274 1106 12-13 0.13061139772817398 1106 14-15 0.2534760684922972 1106 16-17 0.1635204001352406 1106 18-19 0.13324288053602373 1106 20-21 0.17769838910717795 1106 22-23 0.12725281703756508 1106 24-25 0.18938874111686488 1106 26-27 0.2531149474678287 1106 28-29 0.2967561199729829 1106 30-31 0.29038393474756674 1106 32-33 0.24534500504065448 1106 34-35 0.29379533053722184 1106 36-37 0.29706432568885077 1106 38-39 0.3436847382280561 1106 40-41 0.3329146897927089 1106 42-43 0.3697504640475415 1106 44-45 0.31390752736862026 1106 46-47 0.279031515598696 1106 48-49 0.26745404082672053 1106 50-51 0.21585268318612094 1106 52-53 0.3377371214356657 1106 54-55 0.24176798650507436 1106 56-57 0.37849501470368097 1106 58-59 0.2757202855560337 1106 60-61 0.12971336048018145 1106 62-63 0.2688093410740535 1106 64-65 0.4490520776264404 1106 66-67 0.41757884442836257 1106 68-69 0.3195094086996306 1106 70-71 0.4606205272915389 1106 72-73 0.44707959851670154 1106 74-75 0.354508226266411 1106 76-77 0.3170150851641189 1106 78-79 0.7108392576291749 1106 80-81 0.5576448199494983 1106 82-83 0.5853606172178836 1106 84-85 0.5989947325111942 1106 86-87 0.7272107815577087 1106 88-89 0.5609898016077111 1106 90-91 0.7211921181733949 1106 92-93 0.7369554645116985 1106 94-95 0.9105048095121333 1106 96-97 0.932782042301266 1106 98-99 0.935216468516213 1106 100-101 0.7954766388835317 1107 1 0.08519224588697938 1107 2 0.16985318950616346 1107 3 0.05574619601921427 1107 4 0.08276981709067144 1107 5 0.08452536257553334 1107 6 0.08715385791245467 1107 7 0.12056670055397944 1107 8 0.05567942692831451 1107 9 0.005464191330169399 1107 10-11 0.03449217682454986 1107 12-13 0.07257619865143283 1107 14-15 0.10497295215004954 1107 16-17 0.10295763065855112 1107 18-19 0.10283677042645678 1107 20-21 0.13375832179848857 1107 22-23 0.16979626483389865 1107 24-25 0.13586820623140028 1107 26-27 0.17143998967015506 1107 28-29 0.1993351216682413 1107 30-31 0.20188698299263308 1107 32-33 0.14845731521975125 1107 34-35 0.08989712507726466 1107 36-37 0.08464438468251245 1107 38-39 0.09920822638093085 1107 40-41 0.16485863147284618 1107 42-43 0.25282123494352504 1107 44-45 0.20787617757488164 1107 46-47 0.2062551575078473 1107 48-49 0.24910444779700924 1107 50-51 0.2614200044220496 1107 52-53 0.20799325645446487 1107 54-55 0.28314948127414397 1107 56-57 0.24069901595093057 1107 58-59 0.3581390259014725 1107 60-61 0.26006400722423706 1107 62-63 0.21575473429945902 1107 64-65 0.34966988038114977 1107 66-67 0.4193981144982146 1107 68-69 0.38749441298258347 1107 70-71 0.39673210632190603 1107 72-73 0.5107024404631275 1107 74-75 0.4890661142501216 1107 76-77 0.3838240689749064 1107 78-79 0.5708501710991527 1107 80-81 0.609808787219837 1107 82-83 0.6861694504980456 1107 84-85 0.6763878109699029 1107 86-87 0.7930840562796782 1107 88-89 0.8383570748172033 1107 90-91 0.9360584505665095 1107 92-93 1.0484992814664622 1107 94-95 0.9970989825434948 1107 96-97 1.0502203874679772 1107 98-99 1.1394396588335134 1107 100-101 1.7908100104468652 1108 1 0.0396149991479362 1108 2 0.12861749886265983 1108 3 0.08445583164348136 1108 4 0.07196739556097498 1108 5 0.04709575861831894 1108 6 0.0127643628545826 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-0.03793058573051411 2316 68-69 -0.14211545113936097 2316 70-71 -0.08626821321215772 2316 72-73 -0.011784246871535231 2316 74-75 -0.0408982721650446 2316 76-77 0.007052314448632302 2316 78-79 0.039829909362133264 2316 80-81 0.13008979494098938 2316 82-83 0.2561614562941408 2316 84-85 0.1955285034408547 2316 86-87 0.17817017581016614 2316 88-89 0.26014852056756155 2316 90-91 0.28546460377933514 2316 92-93 0.3271659265075897 2316 94-95 0.36950724106946353 2316 96-97 0.5565467355848774 2316 98-99 0.8310207722467027 2316 100-101 1.2238521570068208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 24.0 5 53.0 6 85.0 7 231.0 8 637.0 9 1308.0 10 2196.0 11 3358.0 12 5098.0 13 7072.0 14 8852.0 15 11178.0 16 14579.0 17 19356.0 18 19810.0 19 15676.0 20 14428.0 21 14594.0 22 15775.0 23 18036.0 24 20643.0 25 24469.0 26 30070.0 27 36840.0 28 45298.0 29 56668.0 30 71373.0 31 91758.0 32 118741.0 33 161918.0 34 233582.0 35 356774.0 36 570196.0 37 859968.0 38 436312.0 39 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.98162673878267 23.858872016217575 13.873998352179342 24.28550289282041 2 13.618628389620513 21.812738307438767 41.82543688239337 22.743196420547353 3 16.5712754134559 29.714276500468973 30.166179442644925 23.548268643430205 4 11.291549804395451 18.547339221396847 40.90600850809054 29.255102466117165 5 12.08245601065291 38.57755532494937 36.05034881467507 13.289639849722649 6 27.22391221875096 40.43706869721803 19.578617391174717 12.760401692856288 7 24.906107078545137 32.38012052976143 25.765708373225465 16.948064018467964 8 24.82737240678323 36.12415496370691 20.90408577576621 18.144386853743654 9 25.996497098104072 14.905100077913252 22.99600029084369 36.102402533138985 10-11 24.368578659679116 26.86720278041697 28.917787677415408 19.8464308824885 12-13 24.547420146523155 24.779151983776032 29.036193914856423 21.637233954844387 14-15 21.921366677842077 25.82692978170222 26.76370209961856 25.488001440837145 16-17 20.680446451144192 27.978265214760377 28.30424744348095 23.03704089061448 18-19 20.410153486975467 28.61647848532502 30.079610853580586 20.893757174118928 20-21 21.468751492627447 27.550578964517374 29.229836181380588 21.75083336147459 22-23 21.954789018156127 27.790783601648805 28.507914081854246 21.746513298340822 24-25 21.504102995172786 27.780820075759305 29.50659402876006 21.20848290030785 26-27 21.54237510378039 27.73740648356292 28.650886875496084 22.069331537160604 28-29 20.9184149992592 28.536313933511792 28.708979555453336 21.83629151177567 30-31 22.06318609523792 27.798906537259484 28.527536903835088 21.610370463667508 32-33 22.304534986964512 28.112739061910318 27.947040277141223 21.63568567398395 34-35 22.15497222534058 27.475586319272743 29.081219924983014 21.28822153040366 36-37 22.165148712088783 27.481868664604598 28.7808538452669 21.57212877803972 38-39 22.405465067390004 27.11520523665145 29.526791413166958 20.95253828279159 40-41 21.647941692963165 26.858421187778305 29.746771706629637 21.746865412628892 42-43 22.024275583504384 27.12012791771207 28.835124005853107 22.020472492930434 44-45 21.583540678648554 28.27419384034961 28.735953898226295 21.40631158277554 46-47 22.18947188445109 27.533542095820824 28.033391654185642 22.243594365542446 48-49 22.763747180925485 26.85877900798392 28.522888618529514 21.854585192561082 50-51 22.296499927441193 27.226528595015072 27.687695334544777 22.789276142998958 52-53 21.42471422934485 27.745088285966276 28.01436208031119 22.815835404377687 54-55 21.819802256715796 28.062521657358758 27.539900498602215 22.57777558732323 56-57 22.79504129767395 27.251901360181 27.64152846267511 22.311528879469936 58-59 21.9188594789943 27.370718307849522 28.703062285879266 22.00735992727691 60-61 22.115483197540364 28.67759825177389 27.296455814120463 21.91046273656529 62-63 22.577927702222308 27.62374623097309 26.708988073887685 23.08933799291692 64-65 21.285742057548717 27.86854473805662 27.411378620372627 23.434334584022036 66-67 22.207914555187138 26.72984384799812 27.7448447903773 23.317396806437447 68-69 21.386907229433444 27.37367982688 27.576479715827624 23.662933227858936 70-71 21.682888165589652 27.03180719966522 27.933845551697022 23.351459083048102 72-73 22.45857835240702 26.992727995134757 28.155844147942084 22.39284950451614 74-75 22.32570096478919 27.839381804768042 27.390110245323417 22.444806985119353 76-77 21.882686240814163 27.600059082658067 27.74280667877425 22.77444799775352 78-79 21.469226330746377 27.72589788005792 27.749811633554284 23.055064155641418 80-81 21.160374511233268 27.99445303762515 28.537846639511944 22.307325811629635 82-83 22.214742894956018 26.762153589886932 29.15678064400811 21.866322871148938 84-85 22.917575573927397 27.276131958426657 27.368102724926786 22.438189742719157 86-87 21.398309145454927 28.111696552789994 27.910402171965305 22.579592129789773 88-89 22.081941862294034 26.950287938292462 27.91379892053183 23.053971278881676 90-91 21.769550117006816 26.63992080287485 28.599724124335108 22.99080495578323 92-93 21.98159722259195 27.17980607855399 27.913434629890116 22.925162068963946 94-95 20.470467683869423 27.238559511411665 28.638513935957665 23.65245886876125 96-97 20.969800706231727 27.365589541332717 27.7386483206042 23.925961431831357 98-99 20.287442397890594 28.064301401677948 27.780820075759305 23.867436124672153 100-101 19.265733510476636 27.99255608718152 27.774498848639528 24.967211553702317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 110.0 1 65.5 2 35.5 3 59.0 4 50.0 5 44.0 6 85.5 7 146.0 8 229.0 9 320.0 10 371.5 11 449.0 12 573.0 13 725.5 14 916.0 15 1124.0 16 1384.0 17 1650.0 18 1847.0 19 2087.5 20 2412.5 21 2756.5 22 3114.0 23 3492.5 24 3891.5 25 4483.0 26 5598.5 27 7178.5 28 11024.0 29 20836.0 30 34866.0 31 48414.5 32 65147.0 33 84947.0 34 108220.5 35 125489.0 36 134792.5 37 153255.5 38 153555.0 39 128360.0 40 106653.5 41 91999.5 42 86371.0 43 87091.5 44 91846.5 45 105934.0 46 120146.0 47 129639.0 48 135228.0 49 130706.0 50 124163.0 51 124605.0 52 122621.0 53 116590.0 54 110942.5 55 98000.5 56 83448.5 57 67612.5 58 53665.5 59 49970.0 60 41539.5 61 28846.0 62 20276.0 63 15995.0 64 11746.0 65 5895.5 66 3224.5 67 2169.5 68 1545.5 69 1140.0 70 857.0 71 660.5 72 491.0 73 339.0 74 260.0 75 206.5 76 150.5 77 101.5 78 64.5 79 48.5 80 39.0 81 24.0 82 18.0 83 16.0 84 12.5 85 12.5 86 10.0 87 5.0 88 4.5 89 2.5 90 1.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08065131949026906 2 1.8253787889159349E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.346527779679214E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 1.521148990763279E-5 34-35 0.0 36-37 0.0 38-39 9.126893944579674E-5 40-41 0.003437796719125011 42-43 0.005734731695177562 44-45 1.521148990763279E-5 46-47 0.0 48-49 1.8253787889159349E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002905394572357863 68-69 0.007651379423539294 70-71 0.0013538226017793184 72-73 0.0 74-75 4.563446972289837E-5 76-77 0.0020839741173456923 78-79 0.005400078917209641 80-81 0.0044113320732135095 82-83 0.0046090814420127356 84-85 0.0022817234861449184 86-87 3.6507575778318697E-4 88-89 0.0 90-91 3.042297981526558E-5 92-93 0.0012017077027029904 94-95 0.004015833335615057 96-97 0.00327047033014105 98-99 0.0 100-101 2.4338383852212463E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3286989.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.603403003173174 #Duplication Level Percentage of deduplicated Percentage of total 1 80.41151045643242 23.804543501356427 2 11.634004790711822 6.8881226472057895 3 3.2462821726861013 2.8830299826012977 4 1.3034782197919366 1.5434956418543775 5 0.6781516774459369 1.0037798702354988 6 0.4032017477826456 0.7161686298716067 7 0.2690461483710008 0.5575277089674783 8 0.20178266728752134 0.47787628950141614 9 0.14180627113181438 0.37781533734231093 >10 1.128546924956468 6.947264353600715 >50 0.21332717665400427 4.4845223092073985 >100 0.2802606657766998 18.32748421612752 >500 0.055698002007865594 11.413647810846397 >1k 0.03125276779330236 16.487374231237332 >5k 0.001237733377952569 2.5842676591928924 >10k+ 4.125777926508563E-4 1.5030798108516492 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 13476 0.409980075990519 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 13182 0.4010357199248309 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11590 0.3526023360589281 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 10978 0.33398347241198556 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9943 0.3024956883031857 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 9729 0.29598517062271884 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 8064 0.24533090923030165 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 7901 0.24037196352041335 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7110 0.2163073864865383 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 7075 0.215242582193004 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 6438 0.19586314405067984 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6031 0.18348099126586673 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5958 0.18126011373935236 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5749 0.1749017109579618 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 5636 0.17146391423883683 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5001 0.15214532205614317 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 4649 0.1414364331611697 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 4578 0.13927640159428584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4514 0.13732933088610885 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 4343 0.13212700133769842 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4307 0.13103177406434888 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4290 0.13051458340748937 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 3843 0.11691551143006562 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 3834 0.11664170461172824 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 3774 0.11481632582281232 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 3737 0.11369067556964749 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 3729 0.11344729173112535 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 3717 0.11308221597334217 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 3690 0.11226079551832999 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 3665 0.11150022102294835 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 3615 0.10997907203218509 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 3564 0.10842750006160654 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 3493 0.10626746849472267 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 3456 0.10514181824155784 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 3454 0.10508097228192732 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 3379 0.10279924879578241 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 3376 0.1027079798563366 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 3371 0.10255586495726027 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 3354 0.10203867430040076 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 3337 0.10152148364354124 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 3312 0.10076090914815959 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.0422979815265582E-5 0.0 12-13 0.0 0.0 0.0 3.0422979815265582E-5 0.0 14-15 0.0 0.0 0.0 3.0422979815265582E-5 0.0 16-17 0.0 0.0 0.0 3.0422979815265582E-5 0.0 18-19 0.0 0.0 0.0 3.0422979815265582E-5 0.0 20-21 0.0 0.0 0.0 6.0845959630531164E-5 0.0 22-23 0.0 0.0 0.0 1.5211489907632791E-4 0.0 24-25 0.0 0.0 0.0 2.1296085870685909E-4 0.0 26-27 0.0 0.0 0.0 4.4113320732135097E-4 0.0 28-29 0.0 0.0 0.0 0.0016276294201167086 0.0 30-31 0.0 0.0 0.0 0.006099807452960749 0.0 32-33 0.0 0.0 0.0 0.01907520834417152 0.0 34-35 0.0 0.0 0.0 0.04167948234691385 0.0 36-37 0.0 0.0 0.0 0.07654421721520821 0.0 38-39 0.0 0.0 0.0 0.1244147759545286 0.0 40-41 0.0 0.0 0.0 0.17680314719641593 0.0 42-43 0.0 0.0 0.0 0.23188395215195426 0.0 44-45 0.0 0.0 0.0 0.29983367756935 0.0 46-47 0.0 0.0 0.0 0.38366419845031424 0.0 48-49 0.0 0.0 0.0 0.47472017703740416 0.0 50-51 0.0 0.0 0.0 0.5687423961564824 0.0 52-53 0.0 0.0 0.0 0.6636773046700186 0.0 54-55 0.0 0.0 0.0 0.7691385641996369 0.0 56-57 0.0 0.0 0.0 0.8946181444476997 0.0 58-59 0.0 0.0 0.0 1.0413481760967256 0.0 60-61 0.0 0.0 0.0 1.1967639684830098 0.0 62-63 0.0 0.0 0.0 1.3545679647847924 0.0 64-65 0.0 0.0 0.0 1.522928735082472 0.0 66-67 0.0 0.0 0.0 1.7139394138526172 0.0 68-69 0.0 0.0 0.0 1.9305206071574927 0.0 70-71 0.0 0.0 0.0 2.1637279589314113 0.0 72-73 0.0 0.0 0.0 2.4162082684183 0.0 74-75 0.0 0.0 0.0 2.663501459846686 0.0 76-77 0.0 0.0 0.0 2.9432711822278685 0.0 78-79 0.0 0.0 0.0 3.253783325712377 0.0 80-81 0.0 0.0 0.0 3.5972435563368177 0.0 82-83 0.0 0.0 0.0 3.960022379143952 0.0 84-85 0.0 0.0 0.0 4.3236226224060985 0.0 86-87 0.0 0.0 0.0 4.7194408012926115 0.0 88-89 0.0 0.0 0.0 5.144662790170578 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3415 0.0 48.95878 2 ACCTGGG 3530 0.0 47.498356 3 GTACCTG 3790 0.0 44.645752 1 CCTGGGG 3380 0.0 43.985165 4 CTGGGGG 3155 0.0 43.508797 5 GTACCCG 325 0.0 39.486603 1 TGGGGGG 8835 0.0 31.558048 6 TGAACCG 405 0.0 29.319973 5 CTGTGCG 3880 0.0 29.258007 9 GTACTAG 2810 0.0 28.754906 1 TAGTACT 4140 0.0 28.682585 4 GTACACG 400 0.0 27.329847 1 ACTGTGC 4390 0.0 27.265572 8 GTATCAA 10395 0.0 27.205822 1 CCGTGTT 440 0.0 26.987701 9 GAACCGT 460 0.0 26.846897 6 GGGGGGA 9320 0.0 26.807013 7 GTACTGT 4515 0.0 26.300308 6 CTAGTAC 4830 0.0 25.863495 3 TAGGCAT 3340 0.0 25.740128 5 >>END_MODULE