##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931105_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4453985 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.270126414884647 33.0 33.0 33.0 27.0 33.0 2 31.20607815248592 33.0 33.0 33.0 27.0 33.0 3 31.21641631033782 33.0 33.0 33.0 27.0 33.0 4 34.85668968350814 37.0 37.0 37.0 33.0 37.0 5 34.85627073283812 37.0 37.0 37.0 33.0 37.0 6 34.94650767795581 37.0 37.0 37.0 33.0 37.0 7 34.90963822284988 37.0 37.0 37.0 33.0 37.0 8 34.91307200181411 37.0 37.0 37.0 33.0 37.0 9 34.91744538879228 37.0 37.0 37.0 33.0 37.0 10-11 34.82726490996265 37.0 37.0 37.0 33.0 37.0 12-13 34.75228149623315 37.0 37.0 37.0 33.0 37.0 14-15 36.41049588177778 40.0 37.0 40.0 33.0 40.0 16-17 36.37993078108705 40.0 37.0 40.0 33.0 40.0 18-19 36.32714288440576 40.0 37.0 40.0 33.0 40.0 20-21 36.245247794952164 40.0 37.0 40.0 33.0 40.0 22-23 36.17202291880193 40.0 37.0 40.0 33.0 40.0 24-25 36.04795312512279 40.0 37.0 40.0 33.0 40.0 26-27 35.87052504667169 40.0 37.0 40.0 27.0 40.0 28-29 35.784121185859405 40.0 37.0 40.0 27.0 40.0 30-31 35.66433093061607 40.0 37.0 40.0 27.0 40.0 32-33 35.513868030538944 37.0 37.0 40.0 27.0 40.0 34-35 35.39956870083756 37.0 37.0 40.0 27.0 40.0 36-37 35.20037909871722 37.0 37.0 40.0 27.0 40.0 38-39 35.03432095078901 37.0 37.0 40.0 27.0 40.0 40-41 34.82690882434494 37.0 37.0 40.0 27.0 40.0 42-43 34.661573512259245 37.0 37.0 40.0 24.5 40.0 44-45 34.40954179235 37.0 33.0 40.0 22.0 40.0 46-47 34.25812906868793 37.0 35.0 40.0 22.0 40.0 48-49 34.26038536277065 37.0 33.0 40.0 22.0 40.0 50-51 33.45838165148737 37.0 33.0 38.5 22.0 40.0 52-53 33.57947557075293 37.0 33.0 38.5 22.0 40.0 54-55 34.11403058160276 37.0 33.0 40.0 22.0 40.0 56-57 34.07003806703435 37.0 33.0 40.0 22.0 40.0 58-59 33.929512223323606 37.0 33.0 40.0 22.0 40.0 60-61 33.94778395077667 37.0 33.0 40.0 22.0 40.0 62-63 33.80676113637563 37.0 33.0 40.0 22.0 40.0 64-65 33.60721634670975 37.0 33.0 40.0 22.0 40.0 66-67 33.38802016621071 37.0 33.0 40.0 15.0 40.0 68-69 33.17626586079657 37.0 33.0 40.0 15.0 40.0 70-71 32.95240105209155 37.0 33.0 40.0 15.0 40.0 72-73 32.356712135312534 37.0 33.0 38.5 6.0 40.0 74-75 32.16693242119136 37.0 33.0 37.0 6.0 40.0 76-77 31.813799215758472 37.0 33.0 37.0 6.0 40.0 78-79 31.544282591881206 37.0 33.0 37.0 6.0 38.5 80-81 31.26159068788961 37.0 33.0 37.0 2.0 37.0 82-83 30.99229667365292 37.0 33.0 37.0 2.0 37.0 84-85 30.606010797072734 37.0 33.0 37.0 2.0 37.0 86-87 30.44487812599279 37.0 33.0 37.0 2.0 37.0 88-89 30.241223758050374 35.0 33.0 37.0 2.0 37.0 90-91 29.95536491478979 33.0 33.0 37.0 2.0 37.0 92-93 29.671216000951958 33.0 33.0 37.0 2.0 37.0 94-95 29.43414088731776 33.0 27.0 37.0 2.0 37.0 96-97 29.1540833657949 33.0 27.0 37.0 2.0 37.0 98-99 28.840773487113225 33.0 27.0 37.0 2.0 37.0 100-101 28.14608973761699 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29427295647618834 1101 2 0.25918225249790794 1101 3 0.26834461575793256 1101 4 0.3840050161957791 1101 5 0.2770538509698639 1101 6 0.3151083184684964 1101 7 0.2934108701405549 1101 8 0.24879664380046762 1101 9 0.4170045033590313 1101 10-11 0.289839931463753 1101 12-13 0.36802202372358295 1101 14-15 0.48243309641596 1101 16-17 0.4414764243593723 1101 18-19 0.5071955316325045 1101 20-21 0.5443037408013893 1101 22-23 0.5576863079505614 1101 24-25 0.4280464547858287 1101 26-27 0.5329831497604118 1101 28-29 0.4452467572470056 1101 30-31 0.5012188669714632 1101 32-33 0.555048026985439 1101 34-35 0.4561458148112365 1101 36-37 0.5651762780688117 1101 38-39 0.5075397826025139 1101 40-41 0.5361717041662146 1101 42-43 0.4021419307125953 1101 44-45 0.3311397256093116 1101 46-47 0.39161136742437463 1101 48-49 0.41766604314815226 1101 50-51 0.37214508455844 1101 52-53 0.5240413549174932 1101 54-55 0.6670575317089344 1101 56-57 0.5592346812177809 1101 58-59 0.5958010298282943 1101 60-61 0.6279388863659889 1101 62-63 0.6735429991435637 1101 64-65 0.6338385137760412 1101 66-67 0.7509297169681517 1101 68-69 0.7882515879162568 1101 70-71 0.6510679717774934 1101 72-73 0.637179136145825 1101 74-75 0.6094825978563918 1101 76-77 0.5886602132751939 1101 78-79 0.5668821554169661 1101 80-81 0.5922858481323132 1101 82-83 0.581137918862769 1101 84-85 0.6465718346043836 1101 86-87 0.6934933930173983 1101 88-89 0.654919508515313 1101 90-91 0.5583843512542543 1101 92-93 0.5974906600421939 1101 94-95 0.4120482321475407 1101 96-97 0.36467728194433136 1101 98-99 0.4500614291218774 1101 100-101 0.6233858508745307 1102 1 0.2325548783289797 1102 2 0.2305788256100918 1102 3 0.1932537441725053 1102 4 0.27583403036192067 1102 5 0.13660740044836928 1102 6 0.25988118895565293 1102 7 0.2854368394610134 1102 8 0.3011244854350821 1102 9 0.2565226932177751 1102 10-11 0.23723890207244125 1102 12-13 0.21394962347353186 1102 14-15 0.3293968064947066 1102 16-17 0.28772999069822447 1102 18-19 0.32131435590473245 1102 20-21 0.36627125780066194 1102 22-23 0.3668650209696622 1102 24-25 0.3818989484679136 1102 26-27 0.42354277178736766 1102 28-29 0.38295087490643454 1102 30-31 0.4259864878329509 1102 32-33 0.339129479072092 1102 34-35 0.2729761398139203 1102 36-37 0.36839679154625316 1102 38-39 0.3651652877455476 1102 40-41 0.42187259669676536 1102 42-43 0.4014224933770194 1102 44-45 0.38543476399092214 1102 46-47 0.4149848233452573 1102 48-49 0.47215235377591114 1102 50-51 0.36492553058195654 1102 52-53 0.432722577992358 1102 54-55 0.5260665581137332 1102 56-57 0.5042695934262653 1102 58-59 0.5335146608643839 1102 60-61 0.549370891311689 1102 62-63 0.5888610740254521 1102 64-65 0.4918263121640436 1102 66-67 0.5749691092549014 1102 68-69 0.6325736876158174 1102 70-71 0.6477995146493853 1102 72-73 0.45710526461910916 1102 74-75 0.4245276419679982 1102 76-77 0.44150218541387076 1102 78-79 0.4807906178951704 1102 80-81 0.44435767632386813 1102 82-83 0.4351845105464456 1102 84-85 0.5780603264013706 1102 86-87 0.4750462335452639 1102 88-89 0.5316102914348129 1102 90-91 0.5770202397926418 1102 92-93 0.5406759269300814 1102 94-95 0.42971909380514717 1102 96-97 0.44640673218733085 1102 98-99 0.6060435376943083 1102 100-101 0.3949852504328959 1103 1 0.26446707221300514 1103 2 0.20420627069787756 1103 3 0.17652797466689663 1103 4 0.19321694329709516 1103 5 0.13446033121456935 1103 6 0.2226163481326182 1103 7 0.2461417994246844 1103 8 0.32177708762957025 1103 9 0.2020245330858117 1103 10-11 0.2278659264281515 1103 12-13 0.2887046973019949 1103 14-15 0.3965085809823137 1103 16-17 0.2901258261185191 1103 18-19 0.33576950786915205 1103 20-21 0.32559548195068544 1103 22-23 0.2674520423869282 1103 24-25 0.31952708809753716 1103 26-27 0.35407690117249047 1103 28-29 0.3209346333127385 1103 30-31 0.38965994631738 1103 32-33 0.3275944080485047 1103 34-35 0.3413033625147506 1103 36-37 0.2635877571597547 1103 38-39 0.36736745284615324 1103 40-41 0.4087080236730358 1103 42-43 0.3025454473168736 1103 44-45 0.440019741852403 1103 46-47 0.2238622701026216 1103 48-49 0.2056870374588584 1103 50-51 0.18458674108823914 1103 52-53 0.3437193790929385 1103 54-55 0.488609716542733 1103 56-57 0.5619366006855699 1103 58-59 0.4265568876723691 1103 60-61 0.41995904803773243 1103 62-63 0.45748915355049746 1103 64-65 0.48865245632154597 1103 66-67 0.39405598708899703 1103 68-69 0.4407949926866408 1103 70-71 0.397161738251782 1103 72-73 0.18287977894069485 1103 74-75 0.3171775194214348 1103 76-77 0.23449198766979507 1103 78-79 0.38327761193146515 1103 80-81 0.38239820016869075 1103 82-83 0.39160034075768024 1103 84-85 0.5123843835942203 1103 86-87 0.41749776587818843 1103 88-89 0.410110548548861 1103 90-91 0.39697195577846145 1103 92-93 0.37689594976982477 1103 94-95 0.36805513184466676 1103 96-97 0.3763615201851138 1103 98-99 0.4059603930225819 1103 100-101 0.42917474014499746 1104 1 0.1934850845603151 1104 2 0.15255670557569445 1104 3 0.1028709399146237 1104 4 0.2088769285619776 1104 5 0.15543442349791547 1104 6 0.16150684372189517 1104 7 0.15406543506341563 1104 8 0.15933706081148813 1104 9 0.09112589174365127 1104 10-11 0.11307467170565388 1104 12-13 0.11135474530671274 1104 14-15 0.23984406046024986 1104 16-17 0.23694277088975468 1104 18-19 0.1731639945458241 1104 20-21 0.2005558960519025 1104 22-23 0.10945621428263763 1104 24-25 0.17712248793255725 1104 26-27 0.11239599230643194 1104 28-29 0.07176937232716085 1104 30-31 0.0368967524354602 1104 32-33 0.12444028433200316 1104 34-35 0.20860423235762937 1104 36-37 0.20177459431405254 1104 38-39 0.25418787855922886 1104 40-41 0.16553375585949226 1104 42-43 0.13215023609829757 1104 44-45 0.14278716695435634 1104 46-47 0.03368746837017511 1104 48-49 0.010702494498232795 1104 50-51 0.02467448655897897 1104 52-53 0.12633036246773344 1104 54-55 0.16508338068768325 1104 56-57 0.28347313988782474 1104 58-59 0.17374917873843287 1104 60-61 0.09865939172620841 1104 62-63 0.17059973059730282 1104 64-65 0.2356931104943385 1104 66-67 0.24518509033644165 1104 68-69 0.28489258250519356 1104 70-71 0.2920175561575178 1104 72-73 0.1260205786761759 1104 74-75 0.1533138179239799 1104 76-77 0.14351497715324868 1104 78-79 0.29599130490105097 1104 80-81 0.31104658054927015 1104 82-83 0.15074272043944958 1104 84-85 0.3379716398574075 1104 86-87 0.31291203939733947 1104 88-89 0.250762256762183 1104 90-91 0.3066054732352974 1104 92-93 0.3559381607698633 1104 94-95 0.3087510933850588 1104 96-97 0.2289508850789872 1104 98-99 0.2726783795899834 1104 100-101 0.2780417764545362 1105 1 0.10351476482106747 1105 2 0.01745095143481734 1105 3 0.04873006482564435 1105 4 0.16019588937820828 1105 5 0.10225264126694356 1105 6 0.07624977436666569 1105 7 0.13034245638075959 1105 8 0.14172413636919146 1105 9 0.09741640030430432 1105 10-11 0.13605127300359499 1105 12-13 0.16596756984350947 1105 14-15 0.20804039143053643 1105 16-17 0.20785039159849816 1105 18-19 0.16590565139539137 1105 20-21 0.2715565537631477 1105 22-23 0.26839338620752784 1105 24-25 0.23039657868838503 1105 26-27 0.25143615015713294 1105 28-29 0.23980366905482953 1105 30-31 0.2840743178215348 1105 32-33 0.32970291093100457 1105 34-35 0.3290459395762184 1105 36-37 0.27570008978292293 1105 38-39 0.2572853655194862 1105 40-41 0.3969572636250618 1105 42-43 0.3102683285691654 1105 44-45 0.34352969381436793 1105 46-47 0.302613109534569 1105 48-49 0.40547098430162265 1105 50-51 0.2501640235385807 1105 52-53 0.3773046492881207 1105 54-55 0.4944818246594238 1105 56-57 0.3783822258773242 1105 58-59 0.5038036688665173 1105 60-61 0.5016624887968959 1105 62-63 0.40331784721200137 1105 64-65 0.4663705530798907 1105 66-67 0.48356222945901806 1105 68-69 0.4675223451879873 1105 70-71 0.4480271845506678 1105 72-73 0.40796817407553476 1105 74-75 0.34335427965392284 1105 76-77 0.303764969868471 1105 78-79 0.37022119995864244 1105 80-81 0.3380424011689307 1105 82-83 0.3334637509647429 1105 84-85 0.4926367066484012 1105 86-87 0.41679253576119635 1105 88-89 0.39217465004020724 1105 90-91 0.499447555932079 1105 92-93 0.5257122702579728 1105 94-95 0.3682735450661774 1105 96-97 0.3106355000473293 1105 98-99 0.4339362731656422 1105 100-101 0.4244715075005878 1106 1 0.21646725350885276 1106 2 0.16115853062033736 1106 3 0.25581080577586945 1106 4 0.24026517670852598 1106 5 0.2581067136651356 1106 6 0.2617948818327278 1106 7 0.201772037828718 1106 8 0.2401192155547065 1106 9 0.2454968584491013 1106 10-11 0.2355669687049513 1106 12-13 0.21806033002450675 1106 14-15 0.3172085899599466 1106 16-17 0.4287691557387703 1106 18-19 0.3164942401962918 1106 20-21 0.36542674607084535 1106 22-23 0.3066516582663539 1106 24-25 0.41698629871100934 1106 26-27 0.43279220219332615 1106 28-29 0.5102805497109486 1106 30-31 0.4662399004007227 1106 32-33 0.4327501394377933 1106 34-35 0.400678787426898 1106 36-37 0.5597039076562282 1106 38-39 0.4504538468543231 1106 40-41 0.5888690283309472 1106 42-43 0.5177556023248258 1106 44-45 0.5495322390632325 1106 46-47 0.45790687367484395 1106 48-49 0.4241379102066034 1106 50-51 0.4208035173168625 1106 52-53 0.44147673639219676 1106 54-55 0.5359976617634956 1106 56-57 0.517720461171443 1106 58-59 0.5157610359479605 1106 60-61 0.5260845051996128 1106 62-63 0.4261031271893856 1106 64-65 0.4970620123559044 1106 66-67 0.5602908776490665 1106 68-69 0.5208788921336875 1106 70-71 0.5498053236909399 1106 72-73 0.4311511491886506 1106 74-75 0.468251762685604 1106 76-77 0.41295437880512864 1106 78-79 0.4891285104790093 1106 80-81 0.5758906053544521 1106 82-83 0.6226729562814874 1106 84-85 0.6879527417162734 1106 86-87 0.6739740261458103 1106 88-89 0.7019155295650954 1106 90-91 0.6613448268596791 1106 92-93 0.7185926435611414 1106 94-95 0.7057982905412921 1106 96-97 0.7390362353414481 1106 98-99 0.7833600571022963 1106 100-101 0.8798943016182363 1107 1 -0.0019789156416436526 1107 2 0.11190606399872038 1107 3 0.06244265702466478 1107 4 0.17757425597201149 1107 5 0.20350514266021236 1107 6 0.01085274225354027 1107 7 0.010136203932304966 1107 8 -0.017323760439133196 1107 9 0.030783614943338478 1107 10-11 0.060879724830051885 1107 12-13 0.0913108586980016 1107 14-15 0.14944960348804415 1107 16-17 0.16307203501508383 1107 18-19 0.16699242796454428 1107 20-21 0.2155703911964082 1107 22-23 0.1187725295687656 1107 24-25 0.14708730878239606 1107 26-27 0.1427139999647693 1107 28-29 0.16310245693187397 1107 30-31 0.18556124512399208 1107 32-33 0.20018825100979853 1107 34-35 0.1805882608884275 1107 36-37 0.2658862300188005 1107 38-39 0.1502350700687245 1107 40-41 0.1293102048015342 1107 42-43 0.14996865227038825 1107 44-45 0.14693827829768935 1107 46-47 0.0350758280665886 1107 48-49 -0.02704809077722814 1107 50-51 -0.030973233288449364 1107 52-53 -0.027519466308739027 1107 54-55 0.10551040623588648 1107 56-57 0.04899392301310712 1107 58-59 0.06806781888369784 1107 60-61 0.05685419730986041 1107 62-63 0.06799528659382048 1107 64-65 0.030871992554111216 1107 66-67 -0.030652157390413493 1107 68-69 0.02039482057350739 1107 70-71 0.054355601244523655 1107 72-73 0.11956821392456618 1107 74-75 0.1411518985296638 1107 76-77 0.10657589100684817 1107 78-79 0.12603761910889588 1107 80-81 0.09530642798136668 1107 82-83 0.16782187347839184 1107 84-85 0.22807840529535994 1107 86-87 0.2173548980582325 1107 88-89 0.14734927351975813 1107 90-91 0.2884955201180013 1107 92-93 0.2885921234903144 1107 94-95 0.3332745412920701 1107 96-97 0.3844592797187367 1107 98-99 0.3731246233866905 1107 100-101 0.4613300607495816 1108 1 0.007885565019503105 1108 2 -0.03558455614925293 1108 3 -0.03551513593802369 1108 4 0.016267656188688306 1108 5 0.09066851745641458 1108 6 0.05043217541295064 1108 7 0.059679808495126 1108 8 -0.014254990523070887 1108 9 -0.07805469153135647 1108 10-11 0.013016066076453114 1108 12-13 -0.08356293556079919 1108 14-15 -0.03740249502637738 1108 16-17 -0.007258652913762376 1108 18-19 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-0.7274272336352894 2316 48-49 -0.8990752337767347 2316 50-51 -0.7694753789587452 2316 52-53 -0.9459282015242323 2316 54-55 -0.8450121773123982 2316 56-57 -0.9512544231294626 2316 58-59 -0.9088182220036316 2316 60-61 -0.9401478811252488 2316 62-63 -0.8670158930207421 2316 64-65 -0.9437182935177617 2316 66-67 -0.9260460924769873 2316 68-69 -0.8631309956025177 2316 70-71 -0.8651908823558117 2316 72-73 -0.5166814120419119 2316 74-75 -0.6129957849123286 2316 76-77 -0.7642361057585063 2316 78-79 -0.7888264740815245 2316 80-81 -0.8265156421252939 2316 82-83 -0.7567255431586268 2316 84-85 -0.7435365952782789 2316 86-87 -0.783332731356019 2316 88-89 -0.7914132340287772 2316 90-91 -0.8197134932003074 2316 92-93 -0.7504467011104978 2316 94-95 -0.7229344689751933 2316 96-97 -0.7554491557090905 2316 98-99 -0.7267807224087299 2316 100-101 -0.843713102348417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 110710.0 3 17946.0 4 13377.0 5 11325.0 6 11479.0 7 12169.0 8 12563.0 9 13112.0 10 13437.0 11 13591.0 12 14354.0 13 15102.0 14 16056.0 15 18188.0 16 21612.0 17 26917.0 18 23105.0 19 18340.0 20 18651.0 21 19859.0 22 22101.0 23 25023.0 24 29312.0 25 34397.0 26 38904.0 27 45698.0 28 54692.0 29 66421.0 30 81732.0 31 102959.0 32 132857.0 33 176104.0 34 243390.0 35 354162.0 36 550287.0 37 994991.0 38 1078317.0 39 745.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.32844106318752 24.47816720607369 13.449252519945713 22.74413921079308 2 13.69383605503211 21.992457737554773 44.02023334491885 20.293472862494262 3 17.131887808081544 31.558686341975427 30.1910316065986 21.11839424334443 4 10.570848922569022 18.61428833155812 44.6027192177332 26.212143528139652 5 11.470919010330073 39.16835376903196 37.09023731844238 12.270489902195587 6 24.512228936559058 42.38413465694204 21.1118133536597 11.9918230528392 7 23.248169897294222 33.05267081052137 28.046973665156035 15.65218562702838 8 22.528095626725282 37.54853238167619 22.28020525439578 17.643166737202755 9 25.37940742952659 15.975469158517598 24.696109214557303 33.94901419739851 10-11 22.407916148778263 27.93963786129452 30.93820049645441 18.71424549347281 12-13 23.073959436746712 25.84900432915984 30.58623721425008 20.49079901984337 14-15 20.77044242798383 27.405531873762516 28.18261351122912 23.64141218702453 16-17 18.920377140528462 29.511139701073972 30.09252258335761 21.475960575039952 18-19 18.57041162590216 30.649161667860586 31.251229985269923 19.529196720967324 20-21 19.622270842851965 28.70125292294429 30.335980026874807 21.340496207328943 22-23 19.881645313129702 29.790412405969036 29.511201766507973 20.816740514393288 24-25 19.804983627021645 29.717646107923578 30.369668959370095 20.107701305684685 26-27 19.917018130954638 29.059202040419983 30.125920944951545 20.897858883673834 28-29 19.355565858439128 30.722903197922758 29.202399648853778 20.719131294784336 30-31 20.8410445926513 29.45368024364698 28.9286223460564 20.77665281764532 32-33 20.833781433929392 29.86615356809688 28.8726050940899 20.427459903883825 34-35 21.209658317214807 28.676668197131335 30.04231042538311 20.07136306027075 36-37 21.102215207280672 29.11453451235242 30.19562257169703 19.587627708669878 38-39 21.572378443124528 28.627712037647186 30.809499807475778 18.99040971175251 40-41 20.5077576201306 28.83734991357721 30.674629889634552 19.98026257665764 42-43 21.036647074097363 28.828367049773075 29.516200606445757 20.61878526968381 44-45 20.298137407377222 30.17747380809811 29.543082291169213 19.98130649335545 46-47 21.578871590221354 28.988938984185314 28.79637187552062 20.63581755007271 48-49 21.50355267902641 27.959964854126003 29.800766685684266 20.735715781163325 50-51 21.132144042362853 28.541303693042448 28.6843175554786 21.642234709116092 52-53 19.892289730891047 29.478983565442412 29.094411853897217 21.534314849769316 54-55 20.5987851959484 29.80526527727325 28.509789676136734 21.086159850641618 56-57 21.307239630947755 28.588690104142326 29.108123741205823 20.99594652370409 58-59 21.227843947887745 28.010832585855933 30.203957175006096 20.557366291250222 60-61 20.774921473972544 30.226773666048185 28.68062539837141 20.31767946160786 62-63 21.163556239669763 29.256171014442835 27.766590379227484 21.813682366659922 64-65 19.794163937186806 29.501154114661343 28.308756570619142 22.39592537753271 66-67 20.845718111145665 27.47250137564873 29.227737985541534 22.454042527664072 68-69 19.706376115523987 28.755619465984335 28.542020400499936 22.995984017991745 70-71 20.469547288978323 28.288350338413476 29.291301186866814 21.95080118574139 72-73 21.27518326731288 27.804699593735382 29.96192486028853 20.95819227866321 74-75 20.828392201375593 29.462364207760743 28.448300577945947 21.260943012917714 76-77 20.29935166120434 28.89225852927512 29.655071197054138 21.1533186124664 78-79 20.027800914536975 28.792806462664856 29.321368590036446 21.858024032761726 80-81 20.211779906820265 28.929624446679863 30.089061611617772 20.7695340348821 82-83 21.24811387054143 27.559658660388166 30.525427648400473 20.66679982066993 84-85 22.154487539088635 28.064471860291977 28.882061382933582 20.898979217685802 86-87 20.55560382589185 29.32574868792222 29.04589953747514 21.07274794871079 88-89 21.212730697578515 28.134299598685036 29.147784213744117 21.505185489992336 90-91 21.13881437494984 27.1531745564518 30.265185075289814 21.44282599330855 92-93 21.04603702458539 28.477597619005827 29.188072616181703 21.28829274022707 94-95 19.64721503461207 28.126257987995302 29.931653185328237 22.29487379206439 96-97 20.046720681692925 28.152898366440077 29.177884194859736 22.62249675700726 98-99 19.37510541027365 29.20605544074425 29.27665502562454 22.142184123357566 100-101 20.09605318678754 27.506602869565345 29.13702903643583 23.260314907211285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 96.0 1 60.5 2 39.0 3 54.5 4 46.0 5 45.5 6 95.0 7 158.0 8 223.0 9 286.0 10 283.5 11 308.0 12 457.5 13 683.5 14 878.0 15 1107.5 16 1449.0 17 1805.5 18 2090.5 19 2338.0 20 2638.0 21 2966.5 22 3351.5 23 3761.5 24 4279.5 25 5060.5 26 6479.5 27 9382.5 28 17369.5 29 37900.0 30 66964.0 31 95375.0 32 129650.5 33 171781.5 34 226908.0 35 266534.0 36 287993.0 37 324138.5 38 316748.5 39 247925.0 40 186883.5 41 143883.5 42 117242.5 43 106367.5 44 97931.5 45 100039.0 46 105651.0 47 116649.0 48 133875.0 49 130702.5 50 111938.0 51 104073.0 52 100393.5 53 96151.5 54 91692.5 55 81917.5 56 72596.0 57 60196.5 58 48191.5 59 44643.0 60 38431.5 61 29246.5 62 22240.5 63 18161.5 64 14219.5 65 9256.0 66 6418.0 67 4976.0 68 4003.0 69 3186.0 70 2579.0 71 2024.0 72 1567.5 73 1257.5 74 1035.5 75 880.5 76 700.5 77 572.0 78 458.0 79 365.0 80 321.5 81 259.0 82 209.5 83 169.0 84 140.0 85 121.0 86 99.0 87 83.5 88 71.5 89 57.5 90 48.0 91 40.0 92 33.5 93 21.0 94 13.0 95 11.5 96 11.0 97 7.0 98 5.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.4903608790779496E-4 2 0.003345318854913072 3 2.2451804395389748E-4 4 0.0012797528505372156 5 0.0021553732219574155 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2451804395389747E-5 12-13 6.735541318616923E-5 14-15 0.003176930321947649 16-17 0.004658749412043373 18-19 0.003850484453809341 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.1329214175620258E-4 42-43 1.1225902197694873E-5 44-45 7.184577406524719E-4 46-47 0.7451417101763926 48-49 0.5360929594509187 50-51 0.4131356526795667 52-53 0.3460047575373514 54-55 0.3367209364198577 56-57 0.30254929013007453 58-59 0.3029646485113892 60-61 0.27983928998413776 62-63 0.27461924546220967 64-65 0.27058914657323724 66-67 0.2589927896030184 68-69 0.23829222595046906 70-71 0.2513479502063882 72-73 0.8232403117657559 74-75 0.5462973045486234 76-77 0.640347913160911 78-79 0.35926254803282903 80-81 0.31110342760471804 82-83 0.29214287879281137 84-85 0.28371222624234255 86-87 0.25624244356458314 88-89 0.2601939611381718 90-91 0.27053301706224875 92-93 0.22736942311211197 94-95 0.19403971948715587 96-97 0.15051689666669288 98-99 0.15884651609738246 100-101 0.15414286307654831 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4453985.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.691136158136466 #Duplication Level Percentage of deduplicated Percentage of total 1 79.1816133766064 22.718104506091343 2 10.771693751706604 6.18104264167924 3 3.8693014222303255 3.330439618262441 4 1.7999341233198956 2.0656862003138845 5 1.0135576177108723 1.4540059806929535 6 0.6084376819989726 1.0474061026784076 7 0.4109923537182724 0.8254286306338757 8 0.2980236779512509 0.6840510337958362 9 0.2112989465920112 0.5456166162067979 >10 1.3492294443547883 7.597884592325781 >50 0.18557107598106237 3.7784177996318085 >100 0.22834296338563703 14.269781998972315 >500 0.03895921230591526 7.788284921712626 >1k 0.028943382421588375 16.250905890011698 >5k 0.002760268078353115 5.23589062845401 >10k+ 0.0013407016380572272 6.227052838537068 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 28103 0.630963058923638 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 25849 0.5803566918164296 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 19788 0.4442763053759723 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 18817 0.42247560330804884 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 18782 0.4216897901542102 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 18697 0.41978138678060206 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 17331 0.3891122219764997 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 15797 0.35467115403397187 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 15567 0.34950723902303216 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 13860 0.3111820089201019 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 13824 0.31037374396186784 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 13302 0.2986539020674744 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 11724 0.2632249547315494 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 11471 0.2575446482195158 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11032 0.24768830608993966 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 10900 0.24472466790974823 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 10358 0.23255578992744697 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 9554 0.21450453919355364 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 8933 0.2005619686640166 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 8351 0.18749501850589975 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 8343 0.18731540407073666 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 8069 0.18116360966639986 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 7931 0.17806526065983608 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 7865 0.17658344156974035 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 7649 0.17173385182033615 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 7360 0.16524528035006852 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 7257 0.16293274449734338 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 7094 0.15927310038089487 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 6935 0.1557032634820279 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 6806 0.1528069807150226 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 6701 0.15044954125350668 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 6663 0.14959637268648188 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 6359 0.1427710241502834 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 6218 0.13960531973053344 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6207 0.13935834988218415 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 6174 0.1386174403371363 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6167 0.13846027770636857 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 6155 0.1381908560536239 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 6108 0.13713562124704057 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5949 0.1335657843481736 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 5857 0.13150021834379774 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 5779 0.12974897760095735 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 5718 0.12837941753283857 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 5701 0.12799773685811694 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 5692 0.12779567061855845 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 5668 0.1272568273130691 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 5609 0.1259321708537411 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 5517 0.12386660484936522 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 5362 0.12038657516807982 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 5320 0.11944359938347346 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5224 0.11728822616151605 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 5103 0.11457155782967386 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 4963 0.11142830521431932 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 4937 0.11084455830003918 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4782 0.10736452861875377 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 4725 0.10608477576821654 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 4693 0.10536631802756408 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4557 0.10231287262979108 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 4542 0.10197609556386023 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 4516 0.1013923486495801 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 4473 0.10042692106057834 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 4454 0.10000033677706593 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.2451804395389747E-5 0.0 5 0.0 0.0 0.0 2.2451804395389747E-5 0.0 6 0.0 0.0 0.0 2.2451804395389747E-5 0.0 7 0.0 0.0 0.0 2.2451804395389747E-5 0.0 8 0.0 0.0 0.0 2.2451804395389747E-5 0.0 9 0.0 0.0 0.0 1.5716263076772822E-4 0.0 10-11 0.0 0.0 0.0 1.5716263076772822E-4 0.0 12-13 0.0 0.0 0.0 2.0206623955850773E-4 0.0 14-15 0.0 0.0 0.0 2.0206623955850773E-4 0.0 16-17 0.0 0.0 0.0 2.132921417562026E-4 0.0 18-19 0.0 0.0 0.0 2.2451804395389745E-4 0.0 20-21 0.0 0.0 0.0 2.6942165274467693E-4 0.0 22-23 0.0 0.0 0.0 2.918734571400667E-4 0.0 24-25 0.0 0.0 0.0 4.490360879077949E-4 0.0 26-27 0.0 0.0 0.0 7.745872516409462E-4 0.0 28-29 0.0 0.0 0.0 0.0019645328845966027 0.0 30-31 0.0 0.0 0.0 0.006870252144989262 0.0 32-33 0.0 0.0 0.0 0.023327424766809948 0.0 34-35 0.0 0.0 0.0 0.05153811698961716 0.0 36-37 0.0 0.0 0.0 0.09730612024961915 0.0 38-39 0.0 0.0 0.0 0.16173157296219004 0.0 40-41 0.0 0.0 0.0 0.23046777211867575 0.0 42-43 0.0 0.0 0.0 0.3106768433212056 0.0 44-45 0.0 0.0 0.0 0.40434577125877164 0.0 46-47 0.0 0.0 0.0 0.5212859944521591 0.0 48-49 0.0 0.0 0.0 0.6413694702609012 0.0 50-51 0.0 0.0 0.0 0.7665046020586059 0.0 52-53 0.0 0.0 0.0 0.8949850527112237 0.0 54-55 0.0 0.0 0.0 1.0394287362889636 0.0 56-57 0.0 0.0 0.0 1.221131189260853 0.0 58-59 0.0 0.0 0.0 1.4266325548918553 0.0 60-61 0.0 0.0 0.0 1.6422260065985852 0.0 62-63 0.0 0.0 0.0 1.8573816481196053 0.0 64-65 0.0 0.0 0.0 2.0859859204734637 0.0 66-67 0.0 1.1225902197694873E-5 0.0 2.348436287953372 0.0 68-69 0.0 2.2451804395389747E-5 0.0 2.63966986866817 0.0 70-71 0.0 2.2451804395389747E-5 0.0 2.93878403272575 0.0 72-73 0.0 2.2451804395389747E-5 0.0 3.2807025618631407 0.0 74-75 0.0 2.2451804395389747E-5 0.0 3.610272598583067 0.0 76-77 0.0 2.2451804395389747E-5 0.0 3.9754736488784763 0.0 78-79 0.0 2.2451804395389747E-5 0.0 4.380975688063611 0.0 80-81 0.0 2.2451804395389747E-5 0.0 4.852317643638225 0.0 82-83 0.0 2.2451804395389747E-5 0.0 5.354508378452105 0.0 84-85 0.0 2.2451804395389747E-5 0.0 5.839310190761756 0.0 86-87 0.0 2.2451804395389747E-5 0.0 6.375189858070918 0.0 88-89 0.0 2.2451804395389747E-5 0.0 6.943568512242408 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3365 0.0 48.554817 2 ACCTGGG 3470 0.0 47.083466 3 GTACCCG 345 0.0 45.299477 1 CCTGGGG 3285 0.0 41.517952 4 GTACCTG 3990 0.0 41.067806 1 CTGGGGG 3090 0.0 40.153336 5 TATCACG 215 0.0 35.24357 2 GTACACG 385 0.0 34.442574 1 TGGGGGG 9940 0.0 29.29901 6 GTACTAG 2305 0.0 27.942568 1 TATTCTC 4410 0.0 26.738657 5 CTGTGCG 2900 0.0 26.455757 9 TAGTACT 3105 0.0 26.385378 4 GTACATG 13430 0.0 26.341646 1 TCTCTGC 4460 0.0 26.013298 8 CATGGGG 9785 0.0 25.698782 4 ACATGGG 13400 0.0 25.62194 3 TACATGG 13695 0.0 25.347797 2 ATGGGGG 8745 0.0 25.3434 5 GGGGGGA 10135 0.0 24.810469 7 >>END_MODULE