##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931104_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6503425 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.732125764501014 33.0 33.0 33.0 27.0 33.0 2 31.686843009644917 33.0 33.0 33.0 27.0 33.0 3 31.690332709303174 33.0 33.0 33.0 27.0 33.0 4 35.40302225365865 37.0 37.0 37.0 33.0 37.0 5 35.4072766273156 37.0 37.0 37.0 33.0 37.0 6 35.489970131123215 37.0 37.0 37.0 33.0 37.0 7 35.45928137865817 37.0 37.0 37.0 33.0 37.0 8 35.46780642507602 37.0 37.0 37.0 33.0 37.0 9 35.468876322860645 37.0 37.0 37.0 33.0 37.0 10-11 35.407513271852906 37.0 37.0 37.0 33.0 37.0 12-13 35.3449388591396 37.0 37.0 37.0 33.0 37.0 14-15 37.1886543628934 40.0 37.0 40.0 33.0 40.0 16-17 37.1603182938221 40.0 37.0 40.0 33.0 40.0 18-19 37.09953178517473 40.0 37.0 40.0 33.0 40.0 20-21 37.031817926707845 40.0 37.0 40.0 33.0 40.0 22-23 36.97301298930948 40.0 37.0 40.0 33.0 40.0 24-25 36.8720995475461 40.0 37.0 40.0 33.0 40.0 26-27 36.70680426083179 40.0 37.0 40.0 33.0 40.0 28-29 36.63724929556349 40.0 37.0 40.0 33.0 40.0 30-31 36.54012155133641 40.0 37.0 40.0 33.0 40.0 32-33 36.404066549548894 40.0 37.0 40.0 33.0 40.0 34-35 36.336161330375916 40.0 37.0 40.0 33.0 40.0 36-37 36.17858589896862 40.0 37.0 40.0 33.0 40.0 38-39 36.038832076944075 40.0 37.0 40.0 33.0 40.0 40-41 35.838139441909455 37.0 37.0 40.0 33.0 40.0 42-43 35.707328753695165 37.0 37.0 40.0 27.0 40.0 44-45 35.4997523612558 37.0 37.0 40.0 27.0 40.0 46-47 35.400566163213995 37.0 37.0 40.0 27.0 40.0 48-49 35.43328822889478 37.0 37.0 40.0 27.0 40.0 50-51 34.61988867404483 37.0 35.0 38.5 27.0 40.0 52-53 34.72791644402757 37.0 37.0 38.5 27.0 40.0 54-55 35.28381252955174 37.0 37.0 40.0 27.0 40.0 56-57 35.26703144881351 37.0 37.0 40.0 27.0 40.0 58-59 35.14429681283324 37.0 37.0 40.0 27.0 40.0 60-61 35.18135713105018 37.0 37.0 40.0 27.0 40.0 62-63 35.05171567289544 37.0 37.0 40.0 27.0 40.0 64-65 34.867387645740514 37.0 37.0 40.0 27.0 40.0 66-67 34.673381103034174 37.0 33.0 40.0 27.0 40.0 68-69 34.47945413378335 37.0 33.0 40.0 27.0 40.0 70-71 34.27150555284331 37.0 33.0 40.0 27.0 40.0 72-73 33.65219957176411 37.0 33.0 40.0 22.0 40.0 74-75 33.439486347578395 37.0 33.0 37.0 22.0 40.0 76-77 33.05072450285811 37.0 33.0 37.0 22.0 40.0 78-79 32.76078720059046 37.0 33.0 37.0 22.0 38.5 80-81 32.43976912165513 37.0 33.0 37.0 22.0 37.0 82-83 32.1478080396099 37.0 33.0 37.0 18.5 37.0 84-85 31.752039809792535 37.0 33.0 37.0 15.0 37.0 86-87 31.558946170671607 37.0 33.0 37.0 15.0 37.0 88-89 31.321556410660538 37.0 33.0 37.0 10.5 37.0 90-91 31.004940319908357 37.0 33.0 37.0 4.0 37.0 92-93 30.68605419452058 37.0 33.0 37.0 2.0 37.0 94-95 30.404227849171782 35.0 33.0 37.0 2.0 37.0 96-97 30.060008610847362 33.0 33.0 37.0 2.0 37.0 98-99 29.696178628953206 33.0 33.0 37.0 2.0 37.0 100-101 28.94110564817769 33.0 30.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011912792071314016 1101 2 0.05158274450871758 1101 3 0.02534081765153573 1101 4 0.0808640776111389 1101 5 -0.013040096351979003 1101 6 0.029103959174634042 1101 7 0.07717563990187415 1101 8 -0.037781469738114026 1101 9 0.024579984633341212 1101 10-11 0.010217920665120062 1101 12-13 0.044516151397807846 1101 14-15 0.09469972802370563 1101 16-17 0.10236607477991555 1101 18-19 0.15559095231232334 1101 20-21 0.15616874967097516 1101 22-23 0.12593292560572422 1101 24-25 0.1695227742420471 1101 26-27 0.17860276757271265 1101 28-29 0.13716601815981022 1101 30-31 0.12667063584515148 1101 32-33 0.1060713985021593 1101 34-35 0.1866145188901811 1101 36-37 0.16203477078607165 1101 38-39 0.21766492347901334 1101 40-41 0.1983674166547047 1101 42-43 0.10709682093044393 1101 44-45 0.042856880452887935 1101 46-47 0.0032947168172583474 1101 48-49 0.036809416979608045 1101 50-51 0.017848701673820244 1101 52-53 0.152143965643198 1101 54-55 0.23564120358859242 1101 56-57 0.27297960034358226 1101 58-59 0.24871701533670887 1101 60-61 0.2703830039563897 1101 62-63 0.3175310474396298 1101 64-65 0.3329906173432491 1101 66-67 0.3413737867541258 1101 68-69 0.2727421349256858 1101 70-71 0.21520837938461312 1101 72-73 0.11918325095335547 1101 74-75 0.17523219434674076 1101 76-77 0.10719077365420304 1101 78-79 0.21220228581685063 1101 80-81 0.266420994471666 1101 82-83 0.20801149333973967 1101 84-85 0.32343210954285695 1101 86-87 0.33703322670247005 1101 88-89 0.3475493095426465 1101 90-91 0.27864899415928335 1101 92-93 0.3061128309946426 1101 94-95 0.2807467877721699 1101 96-97 0.21044072174910866 1101 98-99 0.26347850199541156 1101 100-101 0.40535991496840396 1102 1 0.09574598578613802 1102 2 0.048924106240260556 1102 3 0.11422819154136121 1102 4 0.12545262862952455 1102 5 0.1250244967985381 1102 6 0.08924435948502918 1102 7 0.099862569235448 1102 8 0.11304032385248775 1102 9 0.042831438865349014 1102 10-11 0.04920454156098941 1102 12-13 0.08747968597014477 1102 14-15 0.20121955220634646 1102 16-17 0.17157744630119964 1102 18-19 0.1456237271919747 1102 20-21 0.18639362368997325 1102 22-23 0.2219984130512742 1102 24-25 0.19520406495133358 1102 26-27 0.18537830954434753 1102 28-29 0.19083425506131135 1102 30-31 0.21079268814582974 1102 32-33 0.20649493157218757 1102 34-35 0.22501100994669798 1102 36-37 0.19756231462589113 1102 38-39 0.2183720921375354 1102 40-41 0.16194459062994326 1102 42-43 0.19265154992033473 1102 44-45 0.19624630954005795 1102 46-47 0.12131408313256031 1102 48-49 0.24810756535083556 1102 50-51 0.10844882485147167 1102 52-53 0.1359215338918034 1102 54-55 0.18325388468603876 1102 56-57 0.3028695070410592 1102 58-59 0.3461261201486181 1102 60-61 0.2815317998123348 1102 62-63 0.3156007244060106 1102 64-65 0.26958971110988017 1102 66-67 0.3195058977917 1102 68-69 0.23542465910812638 1102 70-71 0.2874200814464771 1102 72-73 0.19013710961741026 1102 74-75 0.2163851439393838 1102 76-77 0.21926189031527343 1102 78-79 0.2281624136464373 1102 80-81 0.24105394096095267 1102 82-83 0.24143385000613193 1102 84-85 0.38357289545821516 1102 86-87 0.2334751725638462 1102 88-89 0.39005398963011473 1102 90-91 0.33763538677701277 1102 92-93 0.25513119643370175 1102 94-95 0.23399198517800457 1102 96-97 0.2246449588073176 1102 98-99 0.261076800027638 1102 100-101 0.24120417930276616 1103 1 0.038185392536586704 1103 2 -0.003990502548163022 1103 3 0.02806504428497547 1103 4 0.033274537638952495 1103 5 0.04856367238922843 1103 6 0.02239020060534358 1103 7 0.11234345410191793 1103 8 0.06691491696414431 1103 9 -0.0014306099285903429 1103 10-11 -0.018489993706289454 1103 12-13 0.03969682372612482 1103 14-15 0.1592463115788405 1103 16-17 0.12427337868788157 1103 18-19 0.11248848240577303 1103 20-21 0.09457965467336749 1103 22-23 0.08608823512720676 1103 24-25 0.11485547459036383 1103 26-27 0.11473980783695481 1103 28-29 0.1408808861891302 1103 30-31 0.15583581740296637 1103 32-33 0.1348585600182659 1103 34-35 0.16767083496202417 1103 36-37 0.1222296369104825 1103 38-39 0.15315557492030507 1103 40-41 0.14619096265562348 1103 42-43 0.12368059185931912 1103 44-45 0.17263600787113376 1103 46-47 0.10124549727141385 1103 48-49 0.03456545036609526 1103 50-51 0.1290715409241514 1103 52-53 0.26778442023160665 1103 54-55 0.35963726876322966 1103 56-57 0.3758235980606699 1103 58-59 0.22485862440347404 1103 60-61 0.2668907869189283 1103 62-63 0.3126183643991851 1103 64-65 0.38423442546247344 1103 66-67 0.37010963481391457 1103 68-69 0.31403907955759536 1103 70-71 0.3018236929093874 1103 72-73 0.17588104847801844 1103 74-75 0.2490053778570882 1103 76-77 0.2889099976721141 1103 78-79 0.2883535831260957 1103 80-81 0.27041079428519765 1103 82-83 0.2198687859745192 1103 84-85 0.2274187841168711 1103 86-87 0.22321828403945787 1103 88-89 0.14899653354865094 1103 90-91 0.14192073299046015 1103 92-93 -0.02489195583054027 1103 94-95 0.023677324859463056 1103 96-97 0.08426283758090491 1103 98-99 0.11064129795373034 1103 100-101 0.2011169409904916 1104 1 0.010356196284941888 1104 2 0.07366259439992362 1104 3 0.06417080583543822 1104 4 0.0669651453361979 1104 5 0.02213266585985707 1104 6 0.008824334163456626 1104 7 0.04516726302686891 1104 8 0.035013377582274074 1104 9 0.044454866042570984 1104 10-11 0.01321352318135638 1104 12-13 0.08377691644931673 1104 14-15 0.14981485298988417 1104 16-17 0.15145806712364873 1104 18-19 0.11530505157939075 1104 20-21 0.12004454527833985 1104 22-23 0.15402062332744038 1104 24-25 0.09689660861413785 1104 26-27 0.20231353943592012 1104 28-29 0.04651191719089809 1104 30-31 0.07152670381364601 1104 32-33 0.06489448685938015 1104 34-35 0.10399678012345959 1104 36-37 0.13234319563914454 1104 38-39 0.1293923317836203 1104 40-41 0.22734639977684878 1104 42-43 0.04994946874616346 1104 44-45 0.032641106287556454 1104 46-47 -0.0027738223279172303 1104 48-49 0.03163120974123501 1104 50-51 0.011938890325424723 1104 52-53 0.10624646592022202 1104 54-55 0.21620222768699193 1104 56-57 0.2557930075620831 1104 58-59 0.1909107846025151 1104 60-61 0.11189641217172408 1104 62-63 0.1739236949635128 1104 64-65 0.19462227899516193 1104 66-67 0.1320542388199044 1104 68-69 0.06575714392680254 1104 70-71 0.11358465195550593 1104 72-73 0.09594331713736892 1104 74-75 0.09409842395697154 1104 76-77 0.06794279408554615 1104 78-79 0.11419813580803151 1104 80-81 0.20530585188030415 1104 82-83 0.08396451384144399 1104 84-85 0.17555918770752044 1104 86-87 0.14788381092680325 1104 88-89 0.15278724015518108 1104 90-91 0.20969908103555568 1104 92-93 0.17428407209518681 1104 94-95 0.2126228023941401 1104 96-97 0.2381373281641288 1104 98-99 0.20808671940583068 1104 100-101 0.3290937873819999 1105 1 0.08327022909804782 1105 2 0.039530132284504305 1105 3 -0.0026291258156625474 1105 4 0.062053759377803885 1105 5 0.02543864682412078 1105 6 0.015813470326094148 1105 7 0.012526282532689947 1105 8 0.026143596326932084 1105 9 0.024498831703141377 1105 10-11 0.020122167498350052 1105 12-13 0.08443985979172197 1105 14-15 0.13665610139739925 1105 16-17 0.07624750614262865 1105 18-19 0.11973453680303692 1105 20-21 0.15377431760324356 1105 22-23 0.11969439925320557 1105 24-25 0.13602693034327018 1105 26-27 0.1637818982246131 1105 28-29 0.07088224526306419 1105 30-31 0.12635677814947854 1105 32-33 0.12542822311306878 1105 34-35 0.10639734310525029 1105 36-37 0.09576398123648033 1105 38-39 0.17521623448020307 1105 40-41 0.12631491547595886 1105 42-43 0.09346130594051516 1105 44-45 0.03770627727125486 1105 46-47 0.04854902862049926 1105 48-49 0.122767461531609 1105 50-51 0.12727033253589326 1105 52-53 0.1759717586766385 1105 54-55 0.2644112072671163 1105 56-57 0.25901913713141056 1105 58-59 0.1746796884659929 1105 60-61 0.2668365031310955 1105 62-63 0.2713635829000083 1105 64-65 0.24940136064520146 1105 66-67 0.258876967954599 1105 68-69 0.21310978966712923 1105 70-71 0.2346701083189089 1105 72-73 0.23098621836885513 1105 74-75 0.312405028720228 1105 76-77 0.2717773005964901 1105 78-79 0.2233367398457915 1105 80-81 0.3657145209186865 1105 82-83 0.3797170447105316 1105 84-85 0.33221234342593675 1105 86-87 0.3891036415352751 1105 88-89 0.31491796588062826 1105 90-91 0.3064889499250505 1105 92-93 0.31981462274702466 1105 94-95 0.27033243280028074 1105 96-97 0.36210130306768207 1105 98-99 0.2596207653814808 1105 100-101 0.1631780491767323 1106 1 0.10170359638476612 1106 2 0.08647493209954504 1106 3 0.14705801800086604 1106 4 0.14915864962910774 1106 5 0.11748559827996274 1106 6 0.1171090849535048 1106 7 0.15651686249596963 1106 8 0.15816365743690852 1106 9 0.1791940614439156 1106 10-11 0.16177319250343203 1106 12-13 0.1646928683538178 1106 14-15 0.2594057716180842 1106 16-17 0.2521121889768523 1106 18-19 0.233298125799692 1106 20-21 0.2958566072028148 1106 22-23 0.3072841312846464 1106 24-25 0.3093945705187977 1106 26-27 0.3384152927168813 1106 28-29 0.3016703906814726 1106 30-31 0.3552752305055691 1106 32-33 0.37101791719751276 1106 34-35 0.3527768856327427 1106 36-37 0.35874533625347027 1106 38-39 0.35572296005040727 1106 40-41 0.47737362912468484 1106 42-43 0.34511073808967296 1106 44-45 0.3802670816007634 1106 46-47 0.39347715960374785 1106 48-49 0.358506963639428 1106 50-51 0.26589301862959047 1106 52-53 0.35880159040465287 1106 54-55 0.46746583365671057 1106 56-57 0.5120717667131416 1106 58-59 0.5249076994418616 1106 60-61 0.4618698528826215 1106 62-63 0.478089581537283 1106 64-65 0.5147526435215681 1106 66-67 0.5675649871533324 1106 68-69 0.6037613779097413 1106 70-71 0.5282876983447267 1106 72-73 0.4002098701830974 1106 74-75 0.5379409191611231 1106 76-77 0.40200230029429207 1106 78-79 0.49984317836530323 1106 80-81 0.5957724926117578 1106 82-83 0.6094800090812029 1106 84-85 0.6639016765395453 1106 86-87 0.6662824152075864 1106 88-89 0.6561568604539119 1106 90-91 0.6273705796140483 1106 92-93 0.6708895939310224 1106 94-95 0.6635658706561749 1106 96-97 0.7255411435311494 1106 98-99 0.6677939983327583 1106 100-101 0.681010805730967 1107 1 0.10002591543498696 1107 2 0.1316233681056893 1107 3 0.1051875234739974 1107 4 0.14962639203891825 1107 5 0.1280223123622264 1107 6 0.05533247902418026 1107 7 0.07351897591125578 1107 8 0.012367649463698172 1107 9 0.07700311363694112 1107 10-11 0.1031013219251875 1107 12-13 0.11926412320121216 1107 14-15 0.1780555613963557 1107 16-17 0.15641738924161075 1107 18-19 0.20314522142366798 1107 20-21 0.2278077427535905 1107 22-23 0.15804076801660472 1107 24-25 0.14308494173922526 1107 26-27 0.13343268524053542 1107 28-29 0.1234057109672051 1107 30-31 0.1821621451498885 1107 32-33 0.17006488041014478 1107 34-35 0.1631053588192941 1107 36-37 0.17616109318845474 1107 38-39 0.1548827982288472 1107 40-41 0.19383834560292712 1107 42-43 0.17990718857090116 1107 44-45 0.15652980662829918 1107 46-47 -0.0689650282000116 1107 48-49 -0.0743078413010565 1107 50-51 -0.01900796253709558 1107 52-53 0.07481019184673698 1107 54-55 0.12234417996416624 1107 56-57 0.09977831031044104 1107 58-59 0.05096845997037036 1107 60-61 0.08199406280854049 1107 62-63 0.10038684623280858 1107 64-65 0.15393687366410091 1107 66-67 0.1785055157487676 1107 68-69 0.12855651968534687 1107 70-71 0.14121012768684693 1107 72-73 0.018064691057979587 1107 74-75 0.10338972397801172 1107 76-77 0.13465068710770112 1107 78-79 0.13783826805981647 1107 80-81 0.21805039027670148 1107 82-83 0.24495638305694456 1107 84-85 0.3075136344215963 1107 86-87 0.24299654707936824 1107 88-89 0.22733013734809404 1107 90-91 0.24875653250164476 1107 92-93 0.27043758604100887 1107 94-95 0.36121709109036004 1107 96-97 0.3883982294184207 1107 98-99 0.3197205181968563 1107 100-101 0.3503518756682986 1108 1 -0.034985439481278746 1108 2 -0.017048718275493258 1108 3 0.01616516754598507 1108 4 0.05034421579344439 1108 5 0.02722166360975109 1108 6 0.009481422219927538 1108 7 0.00804412459826409 1108 8 0.06163818450585268 1108 9 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-0.9960541408871464 2316 54-55 -0.9903649309316194 2316 56-57 -0.9869869684309549 2316 58-59 -0.9492192705353588 2316 60-61 -1.0258316630731201 2316 62-63 -0.9640012428382789 2316 64-65 -0.9722244285433135 2316 66-67 -0.9737070463613833 2316 68-69 -0.9738709412224935 2316 70-71 -0.8957278829145636 2316 72-73 -0.5920297913373957 2316 74-75 -0.7050920452238003 2316 76-77 -0.7541051989431651 2316 78-79 -0.7536986151646943 2316 80-81 -0.7361716872514137 2316 82-83 -0.6708192621941151 2316 84-85 -0.6372736681216651 2316 86-87 -0.6643001208541612 2316 88-89 -0.6370050296387539 2316 90-91 -0.6085011872027373 2316 92-93 -0.6530998480548504 2316 94-95 -0.6029510226548815 2316 96-97 -0.6122919371609825 2316 98-99 -0.5611536764455636 2316 100-101 -0.570376312498599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111633.0 3 18460.0 4 14098.0 5 12328.0 6 12280.0 7 13153.0 8 13331.0 9 13838.0 10 14153.0 11 14588.0 12 14743.0 13 15150.0 14 16005.0 15 17670.0 16 20547.0 17 25733.0 18 23369.0 19 18875.0 20 19828.0 21 21769.0 22 24826.0 23 28841.0 24 34680.0 25 41033.0 26 47186.0 27 56386.0 28 70513.0 29 87777.0 30 110281.0 31 140366.0 32 182376.0 33 242175.0 34 336606.0 35 495761.0 36 787430.0 37 1498201.0 38 1886459.0 39 977.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.904514092575425 25.394821069445484 13.529745798690499 23.17091903928859 2 13.4418463325042 21.81479103159314 44.62164994870205 20.121712687200606 3 16.736916948544255 32.00831132694305 30.027290281688973 21.227481442823724 4 10.370056458516283 19.16992434217456 44.693826682488236 25.766192516820922 5 11.63339400521613 39.207403886680694 37.22572908130919 11.933473026793989 6 24.216224527844943 43.4119252547696 21.036315479920194 11.335534737465258 7 22.9008407108562 32.48888085893202 29.085243544747573 15.525034885464198 8 21.756228448855794 39.20532642415343 21.975805056566347 17.062640070424433 9 25.269715572948098 15.79071027958345 24.75826199271922 34.18131215474923 10-11 22.07573611619604 28.27688587994353 31.157238272829556 18.490139731030876 12-13 22.60208053640708 25.556534186095035 30.613028316336376 21.228356961161506 14-15 20.55525117637896 27.230875970807688 27.496421387623577 24.717451465189775 16-17 19.138799463857307 29.00254254900283 29.97782833300234 21.880829654137525 18-19 18.508722460118566 30.995946984418783 30.80561709593726 19.689713459525393 20-21 19.25507861281391 28.896752780016126 30.037028952977007 21.811139654192957 22-23 19.613065423219304 30.328696033244025 29.15892010748183 20.89931843605485 24-25 19.670582656330772 29.662160936196358 30.064872001232583 20.602384406240283 26-27 20.19676555045995 28.974240496353843 29.251625105233014 21.577368847953192 28-29 19.63561507974644 30.81344061014004 28.589350995821434 20.961593314292085 30-31 21.21555180539485 29.726159677400755 28.32286833476207 20.73542018244233 32-33 20.884718436823675 29.852769886636654 28.561596389594712 20.70091528694496 34-35 21.48567870006958 28.540146153757444 29.54390186709311 20.43027327907987 36-37 21.4996790152881 29.410379915198533 29.444577280433005 19.64536378908037 38-39 22.423576807605222 28.204853596374218 30.466331202404888 18.905238393615672 40-41 20.879455276707056 28.422411189695502 30.714192796027685 19.983940737569757 42-43 21.583087860039242 28.69978625102504 29.44354073231896 20.273585156616754 44-45 20.624499203261315 29.958197167106924 29.410767035700015 20.00653659393175 46-47 21.93629141647022 28.631441082740256 28.450205113384218 20.982062387405303 48-49 21.832432987293743 27.28483677517815 29.767752770913873 21.114977466614228 50-51 21.497181317147522 28.297601093382145 28.228616239170552 21.97660135029978 52-53 20.01576609521129 28.58620033093679 29.330784959595718 22.067248614256204 54-55 20.902708805266027 29.40587876583486 28.225404504094122 21.466007924804995 56-57 21.47584212021933 27.97678696670925 29.223729855259773 21.32364105781165 58-59 20.885401926483325 27.471689208624216 30.769860738282205 20.873048126610254 60-61 20.8298411480031 30.122036506022415 28.08521901061529 20.962903335359194 62-63 21.650298297161747 28.605335975646845 27.10935183014465 22.635013897046754 64-65 20.04593468480362 28.688502537446915 27.989229331685255 23.27633344606421 66-67 21.091112219186876 26.88293722536681 29.07442212131693 22.951528434129386 68-69 19.737686159874702 28.206582938071605 28.37694082063394 23.678790081419756 70-71 20.08971502761396 28.011012442564848 29.15944957826615 22.73982295155504 72-73 21.04062002763689 27.89262312079802 29.342005554511154 21.72475129705394 74-75 21.346452917870202 29.078711168411203 27.823634515964603 21.751201397753988 76-77 20.385211257688123 29.13306187964783 29.145132907432398 21.336593955231653 78-79 20.574013124391605 28.52774350215458 28.469758715387368 22.428484658066452 80-81 20.787726915762597 29.050955408609365 29.31166266447674 20.8496550111513 82-83 21.911310998583808 27.758698100598668 29.416459504320088 20.913531396497437 84-85 22.889804601278716 27.714855210934537 28.19107979421683 21.20426039356992 86-87 21.55845445137815 29.16453868981782 28.13446172501241 21.142545133791614 88-89 22.549017718625848 27.466201740267465 28.520001853004768 21.464778688101912 90-91 22.239753762206124 26.977941094847928 29.22743262194408 21.554872521001876 92-93 21.669788416556194 28.52944467167071 28.316711569111597 21.48405534266149 94-95 21.0301459655763 27.60723186430056 29.007891017956876 22.35473115216627 96-97 21.693194940658824 27.65966587972169 28.11446268946082 22.53267649015867 98-99 20.898479730874357 28.593775699472257 28.54428640862523 21.963458161028157 100-101 21.929917972734373 26.798163154726858 27.940820805485867 23.331098067052906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 42.0 1 28.0 2 17.5 3 26.5 4 26.0 5 23.0 6 39.0 7 69.5 8 124.5 9 151.0 10 141.5 11 165.5 12 323.5 13 590.5 14 786.5 15 1023.5 16 1472.5 17 1780.0 18 1839.0 19 1909.0 20 2073.0 21 2261.5 22 2495.5 23 2839.0 24 3173.5 25 3453.5 26 3986.5 27 5264.0 28 10172.5 29 25039.5 30 46845.5 31 73572.5 32 114266.0 33 178653.0 34 278041.0 35 357418.0 36 422383.0 37 519608.0 38 540539.0 39 442398.5 40 342097.0 41 272359.5 42 221970.5 43 194867.0 44 169588.5 45 155809.5 46 155934.5 47 164485.5 48 174666.5 49 169892.0 50 155238.0 51 151997.0 52 150439.0 53 146286.5 54 140067.0 55 123203.5 56 109643.0 57 91034.0 58 71721.0 59 67418.0 60 57540.5 61 42420.5 62 31910.0 63 26177.0 64 20294.0 65 12416.0 66 8269.5 67 6200.0 68 4857.5 69 3831.0 70 2983.5 71 2343.0 72 1842.0 73 1472.5 74 1201.0 75 1003.5 76 820.0 77 648.5 78 561.5 79 478.5 80 398.0 81 346.0 82 276.0 83 232.0 84 197.5 85 173.0 86 158.5 87 128.0 88 104.0 89 85.5 90 65.5 91 51.5 92 46.0 93 39.5 94 25.5 95 22.0 96 20.0 97 13.5 98 9.0 99 4.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.535544732198803E-4 2 0.0037057396679441986 3 1.6914164459496344E-4 4 0.0010148498675697805 5 0.002121958814009541 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.075302628999335E-5 12-13 9.225907886998006E-5 14-15 0.0031675617078693145 16-17 0.004697524765796484 18-19 0.003728804437661694 20-21 7.688256572498338E-6 22-23 0.0 24-25 4.612953943499003E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.9989467088495677E-4 42-43 3.075302628999335E-5 44-45 5.843074995098736E-4 46-47 0.7469986968405109 48-49 0.5351180339590293 50-51 0.4096456866958564 52-53 0.3448798133291304 54-55 0.3363996663296647 56-57 0.30087992096472244 58-59 0.30122589251048487 60-61 0.2784917178256073 62-63 0.2720797118441437 64-65 0.2687276319785344 66-67 0.2568569638305969 68-69 0.23555280486820407 70-71 0.2493686019289836 72-73 0.825910962300634 74-75 0.5433137154653125 76-77 0.6407700557783014 78-79 0.3579190964760876 80-81 0.30906022595786065 82-83 0.2887170990670301 84-85 0.28061367663961684 86-87 0.25269761702487537 88-89 0.2562265267916521 90-91 0.26658260839480735 92-93 0.22362063066768664 94-95 0.19026897365618883 96-97 0.1466304293506883 98-99 0.15531815927761142 100-101 0.15055912845923494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6503425.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.835130310608708 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86657342762831 16.56556517011628 2 11.794896534772331 5.150862056738019 3 4.42724168418676 2.9000819727233 4 2.1691978114834503 1.8945886753291346 5 1.2723003271999171 1.3890421719320143 6 0.7995852721774381 1.0475429167462735 7 0.5634027463152242 0.8611380668203418 8 0.39949670613253346 0.6978450109650256 9 0.3009316495814864 0.5913793604878372 >10 1.8003076558959896 7.587767722314553 >50 0.23274148746667897 3.581847813409277 >100 0.2692794235265017 12.935964348510065 >500 0.05574613843175741 8.57556127583135 >1k 0.04177414189975974 17.738224160221026 >5k 0.0036171158521014373 5.537378971666632 >10k+ 0.0029078774497286065 12.945210306188981 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 49093 0.7548791598273218 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 47248 0.7265094930748028 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 44182 0.679365103772243 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 37700 0.5796945455663747 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 34582 0.531750577580275 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 33440 0.5141905995686888 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 31051 0.47745610966529173 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 30801 0.4736119813790426 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 29835 0.4587582696809758 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 28926 0.44478101923217384 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 25151 0.3867346821098114 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 24839 0.3819372100085724 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 23617 0.3631471109453865 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 22428 0.34486443681598544 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 21239 0.3265817626865844 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 21210 0.32613584380537947 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 20001 0.3075456394130785 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 18562 0.28541883699742826 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 17994 0.27668497753107013 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 16679 0.25646486274539954 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 16095 0.24748497906872147 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 15526 0.23873574308921836 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 15230 0.23418429519829936 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 14131 0.217285507251948 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 13541 0.20821336449639996 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 13387 0.2058453814720705 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 13281 0.20421547107870083 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 12968 0.1994026224643169 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 12302 0.18916186470974908 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 12284 0.18888508747313915 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 12269 0.18865443977596422 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 11364 0.1747386953797422 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 11135 0.17121747386953798 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 10813 0.16626623663684906 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 10623 0.16334469913929967 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 10614 0.1632063105209947 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 10558 0.1623452257848749 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 10471 0.16100746914126018 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 10383 0.15965433598450046 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 10292 0.15825507328830576 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 10068 0.15481073434382653 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 9545 0.14676881796899327 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 9508 0.1461998869826284 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 9033 0.13889604323875496 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 8780 0.1350057854130708 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 8773 0.13489814982105583 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 8748 0.1345137369924309 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 8710 0.13392942949292103 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 8666 0.1332528629145412 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 8633 0.13274543798075628 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 8536 0.13125391620569163 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 8404 0.12922421647055204 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 8324 0.12799409541895232 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 8279 0.12730215232742748 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 8269 0.1271483871959775 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 8168 0.12559535936833283 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 8114 0.12476502765850302 No Hit ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA 8100 0.12454975647447307 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 8091 0.1244113678561681 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 8052 0.12381168384351324 No Hit CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA 7715 0.11862979891364935 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 7594 0.11676924082310473 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 7534 0.11584665003440495 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 7156 0.1100343280655962 No Hit CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT 6999 0.10762021550183172 No Hit ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA 6989 0.10746645037038174 No Hit ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC 6949 0.1068513898445819 No Hit CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT 6933 0.10660536563426196 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 6910 0.10625170583192703 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 6832 0.10505233780661728 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.5376513144996675E-5 5 0.0 0.0 0.0 0.0 1.5376513144996675E-5 6 0.0 0.0 0.0 0.0 1.5376513144996675E-5 7 0.0 0.0 0.0 0.0 1.5376513144996675E-5 8 0.0 0.0 0.0 0.0 3.075302628999335E-5 9 0.0 0.0 0.0 3.075302628999335E-5 3.075302628999335E-5 10-11 0.0 0.0 0.0 5.381779600748836E-5 3.075302628999335E-5 12-13 0.0 0.0 0.0 7.688256572498338E-5 3.075302628999335E-5 14-15 0.0 0.0 0.0 7.688256572498338E-5 3.075302628999335E-5 16-17 0.0 0.0 0.0 1.0763559201497672E-4 3.075302628999335E-5 18-19 0.0 0.0 0.0 1.0763559201497672E-4 3.075302628999335E-5 20-21 0.0 0.0 0.0 1.845181577399601E-4 3.075302628999335E-5 22-23 0.0 0.0 0.0 3.921010851974152E-4 3.844128286249168E-5 24-25 0.0 0.0 0.0 6.688783218073554E-4 4.612953943499002E-5 26-27 0.0 0.0 0.0 0.0016222221367971491 4.612953943499002E-5 28-29 0.0 0.0 0.0 0.004782095588093966 4.612953943499002E-5 30-31 0.0 0.0 0.0 0.022372826625970163 4.612953943499002E-5 32-33 0.0 0.0 0.0 0.08580863160565394 4.612953943499002E-5 34-35 0.0 0.0 0.0 0.18319577760949038 4.612953943499002E-5 36-37 0.0 0.0 0.0 0.3406358957011113 5.381779600748836E-5 38-39 0.0 0.0 0.0 0.5681698489641996 6.15060525799867E-5 40-41 0.0 0.0 0.0 0.8010471405451742 6.15060525799867E-5 42-43 0.0 0.0 0.0 1.0828063674140935 6.15060525799867E-5 44-45 0.0 0.0 0.0 1.3720923974674883 6.15060525799867E-5 46-47 0.0 0.0 0.0 1.7217235533584228 6.15060525799867E-5 48-49 0.0 0.0 0.0 2.0747529186544016 6.15060525799867E-5 50-51 0.0 7.688256572498338E-6 0.0 2.421124253758596 6.15060525799867E-5 52-53 0.0 1.5376513144996675E-5 0.0 2.7798967467142317 6.15060525799867E-5 54-55 0.0 3.075302628999335E-5 0.0 3.144635326770125 6.15060525799867E-5 56-57 0.0 3.075302628999335E-5 1.5376513144996675E-5 3.587155998569984 6.15060525799867E-5 58-59 0.0 3.075302628999335E-5 1.5376513144996675E-5 4.097348704720974 6.15060525799867E-5 60-61 0.0 3.075302628999335E-5 1.5376513144996675E-5 4.60131392304824 6.15060525799867E-5 62-63 0.0 3.075302628999335E-5 1.5376513144996675E-5 5.086635119187198 6.15060525799867E-5 64-65 0.0 3.075302628999335E-5 1.5376513144996675E-5 5.60686868842187 6.15060525799867E-5 66-67 0.0 4.612953943499002E-5 1.5376513144996675E-5 6.148321845796637 6.15060525799867E-5 68-69 0.0 4.612953943499002E-5 1.5376513144996675E-5 6.712747513810031 6.15060525799867E-5 70-71 0.0 4.612953943499002E-5 1.5376513144996675E-5 7.265033424695449 6.15060525799867E-5 72-73 0.0 4.612953943499002E-5 1.5376513144996675E-5 7.904719436297029 6.15060525799867E-5 74-75 0.0 4.612953943499002E-5 1.5376513144996675E-5 8.484144892883364 6.15060525799867E-5 76-77 0.0 6.15060525799867E-5 1.5376513144996675E-5 9.130881035761925 6.15060525799867E-5 78-79 0.0 6.15060525799867E-5 1.5376513144996675E-5 9.837393373491661 6.15060525799867E-5 80-81 0.0 6.15060525799867E-5 1.5376513144996675E-5 10.62879175203835 6.15060525799867E-5 82-83 0.0 6.15060525799867E-5 1.5376513144996675E-5 11.473700396329626 6.15060525799867E-5 84-85 0.0 6.15060525799867E-5 1.5376513144996675E-5 12.250644852520018 6.15060525799867E-5 86-87 0.0 6.15060525799867E-5 1.5376513144996675E-5 13.107285776340929 6.15060525799867E-5 88-89 0.0 6.15060525799867E-5 1.5376513144996675E-5 13.98612269688664 6.15060525799867E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3785 0.0 49.664967 3 TACCTGG 3940 0.0 47.71298 2 CCTGGGG 3850 0.0 42.92301 4 CTGGGGG 3745 0.0 40.07956 5 GTACCTG 5125 0.0 36.773197 1 TATTCGC 2415 0.0 35.09712 9 GTACCCG 505 0.0 33.756184 1 CCGTGTT 1070 0.0 32.747902 9 GAACCGT 1110 0.0 32.418488 6 TGAACCG 1130 0.0 31.84569 5 AACCGTG 1165 0.0 30.888 7 ACCGTGT 1150 0.0 30.879406 8 TGGGGGG 11835 0.0 30.045113 6 TATTCTC 5820 0.0 30.020567 5 CTTAATA 3415 0.0 29.673262 2 GTACTAG 4030 0.0 29.609953 1 TAGGCAT 4620 0.0 28.901651 5 TCTCTGC 6070 0.0 28.471455 8 CTGTGCG 4575 0.0 28.255766 9 CCTTAAT 3260 0.0 27.598038 1 >>END_MODULE