FastQCFastQC Report
Sat 18 Jun 2016
SRR1931103_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931103_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947944
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC288970.7319506051757573No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC266600.6752882006431702No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT245230.6211587601039933No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG243240.616118161757107No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA205870.5214612973233662No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA182110.461278072839939No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA171960.435568488306825No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA154940.3924574411389827No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC129540.3281201557063626No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA129240.32736026650833955No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC127700.3234595019584877No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC98570.24967426083044747No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA98220.24878772343275385No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG93640.23718674834293496No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT91010.23052505304026602No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT90410.22900527464421988No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA88760.224825884055093No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT83930.21259166796692153No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA83500.2115024934497551No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG81730.207019147181419No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG81060.2053220613058341No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC77550.19643135768896416No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA74710.18923773994767912No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG74600.18895911390840398No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT72740.18424780088066092No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT71850.1819934629265258No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC70450.17844731333575148No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC69720.176598249620562No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA69590.17626896430141867No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA68870.1744452302261633No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA66400.16818880916244No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC62820.15912079806603133No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC59020.14949553489107242No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA57490.14562009998115474No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA57170.14480955150326347No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA53840.13637478140520737No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA53680.13596950716626172No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG50800.12867457086524023No Hit
GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA50430.12773737418767844No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT48650.12322869827940822No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA48140.121936886642769No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA46890.1187706816510062No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC46320.11732689217476236No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC45550.11537650989983649No Hit
GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC43370.10985464839420214No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC42860.10856283675756291No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC40110.10159718577568476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG34750.050.6999632
ACCTGGG35300.049.506893
ATAATGA33300.044.5157934
CCTGGGG34400.043.7806934
CTGGGGG32000.043.363685
ATTAATA27750.041.3020742
GTACCCG3150.040.5955281
GTACACG4900.035.762731
TATAATG48350.035.4589653
GTTATAA49750.035.318791
TATTCGC15400.033.5224889
GTACCTG53950.033.183741
TTATAAT52950.032.9155652
GAACCGT9900.032.0506676
CCGTGTT9850.031.7349769
CTTAATA18950.031.4905932
AACCGTG10150.031.2612427
TTAATAG40650.030.8744563
TGAACCG10750.030.838845
GTACTAG29800.030.6736891
Produced by FastQC (version 0.11.5)