FastQCFastQC Report
Sat 18 Jun 2016
SRR1931103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947944
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC321870.8152851205589543No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC286450.7255675359123634No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA225880.5721459068315052No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG219770.5566694968317686No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT214480.5432701173066284No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA164490.4166472472760505No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA161980.41028950765259087No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA137830.3491184272117335No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA136390.3454709590612227No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC127110.32196505320237573No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT123190.3120358343482076No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC117970.2988137623026061No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC115030.29136684816198No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA108030.2736361002081083No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT97380.24666003367828926No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC95410.24167009461127106No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT91090.23072769015973885No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG89610.22697890344949168No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG84950.21517529124019996No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT83170.21066661533192974No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG81870.20737376214049644No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA80490.2038782718295903No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT79170.2005347593582888No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG78100.19782448788533982No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT76860.19468361253351113No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA75490.1912134518625391No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC75150.1903522441047796No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA74820.18951636598695423No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA69910.17707951277930994No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC67040.16980990611822255No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC66590.16867007232118794No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA65570.1660864490479095No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT65450.16578249336870027RNA PCR Primer, Index 27 (95% over 23bp)
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA64590.16360414433436746No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC63550.1609698617812208No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA62670.1587408534670198No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA59560.15086333544751396No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC57210.14491087006299988No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA55030.13938900855736555No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA51890.1314355016180574No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA51610.13072627169990253No Hit
GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA50070.12682550715005075No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC49420.1251790805543341No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT48190.12206353484243951No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG47910.12135430492428464No Hit
ATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATA44400.1124636013074147No Hit
ATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCT42080.10658712484270293No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA40810.10337026057107193No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC40090.10154652649581655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG30600.051.0678023
TACCTGG31200.050.846912
GTACCCG2550.050.3273931
CTGGGGG25500.048.801575
CCTGGGG30550.044.4659464
ATTAATA32550.043.9226152
ATAATGA32450.040.5450444
TATTCGC15350.034.6563159
CCGTGTT10600.034.5030569
GTACTAG30150.034.3676641
TATAATG44100.033.711533
GTTATAA47200.033.6345251
TTAATAG44300.033.4521143
GAACCGT10600.033.1587836
ATAGCAT43800.033.074896
TGAACCG11200.032.654685
GTACCTG50850.031.6876141
ACCGTGT11100.031.6651428
TTATAAT49800.031.4743882
AACCGTG11300.031.1046967
Produced by FastQC (version 0.11.5)