##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931103_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3947944 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62865835989568 33.0 33.0 33.0 33.0 33.0 2 32.59309022620381 33.0 33.0 33.0 33.0 33.0 3 32.638060468942825 33.0 33.0 33.0 33.0 33.0 4 36.57931951415724 37.0 37.0 37.0 37.0 37.0 5 36.53161265711976 37.0 37.0 37.0 37.0 37.0 6 36.53474973302559 37.0 37.0 37.0 37.0 37.0 7 36.47206976593387 37.0 37.0 37.0 37.0 37.0 8 36.48856164119856 37.0 37.0 37.0 37.0 37.0 9 36.475425436632335 37.0 37.0 37.0 37.0 37.0 10-11 36.45260064479132 37.0 37.0 37.0 37.0 37.0 12-13 36.37638983734318 37.0 37.0 37.0 37.0 37.0 14-15 38.298363907897375 40.0 37.0 40.0 37.0 40.0 16-17 38.2939340071693 40.0 37.0 40.0 37.0 40.0 18-19 38.236676102801866 40.0 37.0 40.0 37.0 40.0 20-21 38.159402083717495 40.0 37.0 40.0 33.0 40.0 22-23 38.02868543728077 40.0 37.0 40.0 33.0 40.0 24-25 38.18505062381837 40.0 37.0 40.0 37.0 40.0 26-27 38.09940275241999 40.0 37.0 40.0 35.0 40.0 28-29 38.03966532959941 40.0 37.0 40.0 33.0 40.0 30-31 37.94246853552127 40.0 37.0 40.0 33.0 40.0 32-33 37.81900148533009 40.0 37.0 40.0 33.0 40.0 34-35 37.77285024812915 40.0 37.0 40.0 33.0 40.0 36-37 37.729073411375644 40.0 37.0 40.0 33.0 40.0 38-39 37.577720074043604 40.0 37.0 40.0 33.0 40.0 40-41 37.438398568976666 40.0 37.0 40.0 33.0 40.0 42-43 37.30706020652775 40.0 37.0 40.0 33.0 40.0 44-45 37.16051823430119 40.0 37.0 40.0 33.0 40.0 46-47 36.985733460251716 40.0 37.0 40.0 33.0 40.0 48-49 36.823260031044 38.5 37.0 40.0 33.0 40.0 50-51 36.62219778193409 37.0 37.0 40.0 33.0 40.0 52-53 36.42879040330866 37.0 37.0 40.0 33.0 40.0 54-55 36.23267756584187 37.0 37.0 40.0 33.0 40.0 56-57 35.952859513711445 37.0 37.0 40.0 33.0 40.0 58-59 35.691792740727834 37.0 37.0 40.0 30.0 40.0 60-61 35.417295306113765 37.0 33.0 40.0 27.0 40.0 62-63 35.10462154478382 37.0 33.0 40.0 27.0 40.0 64-65 34.73944197283447 37.0 33.0 40.0 27.0 40.0 66-67 34.35948369581737 37.0 33.0 38.5 27.0 40.0 68-69 34.02414851882398 37.0 33.0 37.0 27.0 40.0 70-71 33.71908998709202 37.0 33.0 37.0 27.0 40.0 72-73 33.33327613056315 37.0 33.0 37.0 27.0 40.0 74-75 32.87880932454969 37.0 33.0 37.0 27.0 38.5 76-77 31.17815323621612 33.0 30.0 37.0 22.0 37.0 78-79 31.918559508442875 33.0 33.0 37.0 22.0 37.0 80-81 31.886064240019614 33.0 33.0 37.0 22.0 37.0 82-83 31.60525415254117 33.0 33.0 37.0 22.0 37.0 84-85 31.24913942548324 33.0 33.0 37.0 22.0 37.0 86-87 30.845544035072436 33.0 33.0 37.0 15.0 37.0 88-89 30.449049935865354 33.0 33.0 37.0 15.0 37.0 90-91 29.969727787425555 33.0 27.0 37.0 15.0 37.0 92-93 29.544201741463404 33.0 27.0 37.0 4.0 37.0 94-95 29.02036047117183 33.0 27.0 37.0 2.0 37.0 96-97 28.453765681580084 33.0 27.0 37.0 2.0 37.0 98-99 27.71085595439044 33.0 27.0 37.0 2.0 37.0 100-101 25.859807915208524 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.01580296835752648 1101 2 0.1049109217346853 1101 3 0.06967658516069264 1101 4 0.1361685302582032 1101 5 0.054156325407916484 1101 6 0.04252343531886993 1101 7 0.07422703525477203 1101 8 0.11887497544666559 1101 9 0.1034630947071804 1101 10-11 0.141919224204905 1101 12-13 0.14579547509192992 1101 14-15 0.19083445965048185 1101 16-17 0.151174158534765 1101 18-19 0.1350892871622804 1101 20-21 0.11486973229952469 1101 22-23 0.09303539398541716 1101 24-25 0.16996401405229022 1101 26-27 0.1518567169614471 1101 28-29 0.05805907549019196 1101 30-31 -0.03607379263009136 1101 32-33 -0.01766735243305817 1101 34-35 0.027590628844372134 1101 36-37 -0.09104347683341274 1101 38-39 -0.12990320521523557 1101 40-41 -0.16341416842055878 1101 42-43 -0.41875156536378455 1101 44-45 -0.45813776826805963 1101 46-47 -0.497450458389082 1101 48-49 -0.5680641773839952 1101 50-51 -0.4196321040400903 1101 52-53 -0.5218007275787357 1101 54-55 -0.6232494291072754 1101 56-57 -0.565458641075054 1101 58-59 -0.6626938135879143 1101 60-61 -0.7337278110078103 1101 62-63 -0.7492989522232492 1101 64-65 -0.7009801176117278 1101 66-67 -0.9536326069044279 1101 68-69 -1.039141342916949 1101 70-71 -1.0606524494703464 1101 72-73 -1.0996439787885421 1101 74-75 -1.219212411838356 1101 76-77 -1.4146286675089748 1101 78-79 -1.2740843056657418 1101 80-81 -1.5061213197696155 1101 82-83 -1.7430727124880825 1101 84-85 -1.841710483879691 1101 86-87 -1.9652714785543104 1101 88-89 -2.213946508386652 1101 90-91 -2.53417061093419 1101 92-93 -2.832375401771472 1101 94-95 -3.187019352321034 1101 96-97 -3.4374268046468117 1101 98-99 -3.734142046039217 1101 100-101 -5.010323747417225 1102 1 0.0936667161621969 1102 2 0.185306495980754 1102 3 0.1597028968075307 1102 4 0.15063621675346184 1102 5 0.15215034344375766 1102 6 0.10098831247063345 1102 7 0.10725991529891843 1102 8 0.09872226064173617 1102 9 0.15346378666370697 1102 10-11 0.15019268888312354 1102 12-13 0.17810556329908422 1102 14-15 0.19401642172780242 1102 16-17 0.2193780642833687 1102 18-19 0.1998475953977774 1102 20-21 0.02809354044765655 1102 22-23 0.1165454385993101 1102 24-25 0.20087505266173622 1102 26-27 0.14474919902679773 1102 28-29 0.2152483307892865 1102 30-31 0.17553533496841567 1102 32-33 0.24422590981327374 1102 34-35 0.22850786046470972 1102 36-37 0.20717953556471969 1102 38-39 0.21172732113879533 1102 40-41 0.11793928989165892 1102 42-43 0.07741426028739085 1102 44-45 -0.036183847382915246 1102 46-47 -0.004697405563391044 1102 48-49 0.01381611932696103 1102 50-51 -0.12673317812960505 1102 52-53 -0.09145709331927776 1102 54-55 -0.09519807382392287 1102 56-57 -0.08114118699069195 1102 58-59 -0.24919214164795989 1102 60-61 -0.3141656810964335 1102 62-63 -0.4121973245685169 1102 64-65 -0.456703765744912 1102 66-67 -0.311180295683144 1102 68-69 -0.36920170747424663 1102 70-71 -0.4310987268307329 1102 72-73 -0.5009026477063756 1102 74-75 -0.5532856917641809 1102 76-77 -0.7449062631676391 1102 78-79 -0.5539181842031518 1102 80-81 -0.7895412197472957 1102 82-83 -0.8533589922011302 1102 84-85 -0.9440440207635667 1102 86-87 -0.9563522454497253 1102 88-89 -1.1379382412360783 1102 90-91 -1.2620362191380252 1102 92-93 -1.4651719354152668 1102 94-95 -1.6581206097790258 1102 96-97 -2.0469825945065345 1102 98-99 -2.066650769016153 1102 100-101 -3.011255856102835 1103 1 0.09552089698882327 1103 2 0.16414671677114967 1103 3 0.06989766248008067 1103 4 0.1696198156885771 1103 5 0.1086533761635522 1103 6 0.06899404695064959 1103 7 0.11361094878073175 1103 8 0.11596517366717762 1103 9 0.06641060226755968 1103 10-11 0.09284784838142457 1103 12-13 0.09803167052030659 1103 14-15 0.1681292886287764 1103 16-17 0.17252900122185366 1103 18-19 0.1609987535141144 1103 20-21 0.06477019778826332 1103 22-23 0.04935422791690769 1103 24-25 0.11623311055867447 1103 26-27 0.17362404948958243 1103 28-29 0.19127224705812296 1103 30-31 0.2348277918346966 1103 32-33 0.16713489108742152 1103 34-35 0.09119709265464593 1103 36-37 0.07814977987745664 1103 38-39 0.09628325411382832 1103 40-41 0.02668481588328575 1103 42-43 0.013035640921565061 1103 44-45 0.021455668221435076 1103 46-47 -0.00557364121306847 1103 48-49 0.0018844213106206098 1103 50-51 -0.0233839883347855 1103 52-53 -0.08208738564537299 1103 54-55 -0.10105916605509435 1103 56-57 -0.1896832132059174 1103 58-59 -0.12945250189341806 1103 60-61 -0.012661429476743535 1103 62-63 -0.07735813863219931 1103 64-65 -0.06349687242206414 1103 66-67 -0.023382169721550383 1103 68-69 -0.00980915551762962 1103 70-71 -0.06421752840190464 1103 72-73 -0.1915662377943832 1103 74-75 -0.41915613158523257 1103 76-77 -0.47429551360071187 1103 78-79 -0.396846022100771 1103 80-81 -0.47951895666188094 1103 82-83 -0.4186679308541379 1103 84-85 -0.39671813375155196 1103 86-87 -0.3745185760444336 1103 88-89 -0.5320976523540608 1103 90-91 -0.46501069811886353 1103 92-93 -0.5630442286099289 1103 94-95 -0.7357673984217854 1103 96-97 -0.9329734390346438 1103 98-99 -0.9000214631110275 1103 100-101 -1.8482137483585426 1104 1 0.06865828289562614 1104 2 0.1824565582661748 1104 3 0.12080843767790839 1104 4 0.1533759961407668 1104 5 0.09279183262230362 1104 6 0.07912398795386366 1104 7 0.0904095864989074 1104 8 0.1386218560786574 1104 9 0.09855492437174007 1104 10-11 0.11331901960583934 1104 12-13 0.11528275226736184 1104 14-15 0.15999857202769618 1104 16-17 0.15188766418427946 1104 18-19 0.10482119713101667 1104 20-21 0.08963776699052062 1104 22-23 0.1611841403203229 1104 24-25 0.21870367933640722 1104 26-27 0.19870305717808634 1104 28-29 0.2037277875077379 1104 30-31 0.13741428234094144 1104 32-33 0.18383121735678287 1104 34-35 0.14852535803210998 1104 36-37 0.10683406378091576 1104 38-39 0.1434804589761498 1104 40-41 0.1343588014711372 1104 42-43 0.0721362429503074 1104 44-45 0.07247331949137958 1104 46-47 0.06516354097242782 1104 48-49 0.044062331758020434 1104 50-51 0.10664276949299278 1104 52-53 0.10222905828319284 1104 54-55 0.028336933386228225 1104 56-57 0.10141683115660527 1104 58-59 0.12033225327977704 1104 60-61 0.14827617221225609 1104 62-63 0.10420870936555104 1104 64-65 0.1480472211225603 1104 66-67 0.2604953807505268 1104 68-69 0.14455885163769722 1104 70-71 0.13797050541869993 1104 72-73 0.0015599440370479556 1104 74-75 -0.20100602205219076 1104 76-77 -0.014672203989089638 1104 78-79 0.1648002007853968 1104 80-81 0.08087990603341311 1104 82-83 -0.030599494940677374 1104 84-85 0.14172374148764888 1104 86-87 0.11187062182974472 1104 88-89 0.039714186648780014 1104 90-91 -0.095425254878716 1104 92-93 -0.07471469266105757 1104 94-95 -0.17162108149661748 1104 96-97 -0.19768658723951305 1104 98-99 -0.15713909605083032 1104 100-101 -0.720512739245418 1105 1 0.07289758805795543 1105 2 0.18913445530868245 1105 3 0.08362022791315837 1105 4 0.11167074527208598 1105 5 0.09249665908233595 1105 6 0.09219684294293273 1105 7 0.13002120055816846 1105 8 0.09710532502447933 1105 9 0.14322709822572222 1105 10-11 0.040324529746300186 1105 12-13 0.045207935126590826 1105 14-15 0.02186217175831473 1105 16-17 0.10288101666240124 1105 18-19 0.13967575701376944 1105 20-21 0.03010060303870432 1105 22-23 0.042504091984426395 1105 24-25 0.16847808743893466 1105 26-27 0.16010700744099182 1105 28-29 0.11046163252207464 1105 30-31 0.09820566664858887 1105 32-33 0.15690640532776712 1105 34-35 0.17289180016157246 1105 36-37 0.10370440159640992 1105 38-39 0.12241805501478353 1105 40-41 0.09665562005162798 1105 42-43 0.14649255299136854 1105 44-45 0.09013683318713106 1105 46-47 0.0029051272960600727 1105 48-49 0.03315592258280731 1105 50-51 0.08024857373823124 1105 52-53 -0.0589023662237409 1105 54-55 0.040300743057088084 1105 56-57 0.07798403882475924 1105 58-59 0.012461722947563203 1105 60-61 0.030988284072755334 1105 62-63 -0.22157330012360177 1105 64-65 -0.1354872844392574 1105 66-67 0.06833571872493138 1105 68-69 -0.09468004078793513 1105 70-71 -0.14300991933026097 1105 72-73 -0.2209741861945318 1105 74-75 -0.15719479446072882 1105 76-77 -0.24538643114277647 1105 78-79 -0.11922498332540599 1105 80-81 -0.15226496764314135 1105 82-83 -0.10129746012763619 1105 84-85 0.004132934139501998 1105 86-87 -0.016728044953360666 1105 88-89 -0.017525854970831034 1105 90-91 0.0018947550044678962 1105 92-93 -0.020765751636560026 1105 94-95 -0.06058647081701807 1105 96-97 0.019529567682862847 1105 98-99 -0.18517590653971183 1105 100-101 -1.1380398599843318 1106 1 0.1361852370850798 1106 2 0.2153173465443814 1106 3 0.10827926267246113 1106 4 0.13008529561480486 1106 5 0.15238644367082088 1106 6 0.12097050641750684 1106 7 0.0972233757940586 1106 8 0.15904907590857675 1106 9 0.12767290886547045 1106 10-11 0.14526468592781328 1106 12-13 0.16640310677475867 1106 14-15 0.27086294962472124 1106 16-17 0.27306447372058074 1106 18-19 0.1850406720580864 1106 20-21 0.190722622740374 1106 22-23 0.20899320673729704 1106 24-25 0.19143351664234132 1106 26-27 0.22120146659366213 1106 28-29 0.27368499178336236 1106 30-31 0.2639626369649477 1106 32-33 0.22365713625155337 1106 34-35 0.3135015464259041 1106 36-37 0.2429638674744936 1106 38-39 0.36040852168719795 1106 40-41 0.36409557046682295 1106 42-43 0.3659958582260927 1106 44-45 0.42343748804016457 1106 46-47 0.43230150938024536 1106 48-49 0.35611720391962365 1106 50-51 0.4142373599625273 1106 52-53 0.3978874058932078 1106 54-55 0.40155363529441246 1106 56-57 0.45712231322801955 1106 58-59 0.3317309986473589 1106 60-61 0.34976291848531105 1106 62-63 0.3523602444724503 1106 64-65 0.6273344678080619 1106 66-67 0.6721131208687581 1106 68-69 0.5353965279953528 1106 70-71 0.6246421796395722 1106 72-73 0.7598020438485733 1106 74-75 0.6856963491619226 1106 76-77 0.5617371567074407 1106 78-79 0.7230974780135391 1106 80-81 0.6980684644580286 1106 82-83 0.7601042464911778 1106 84-85 0.9592712746669854 1106 86-87 1.0361695213682616 1106 88-89 1.0794189896135258 1106 90-91 1.0474761083078725 1106 92-93 1.0781299668338313 1106 94-95 1.21456124149406 1106 96-97 1.1299384344113008 1106 98-99 1.2286275742171604 1106 100-101 1.1925727544531242 1107 1 6.467235529825643E-4 1107 2 0.07707005512578036 1107 3 0.06703887582769852 1107 4 0.036027276759853066 1107 5 0.04764107193594924 1107 6 0.06369555347627909 1107 7 0.09122048349676959 1107 8 0.033133149017679386 1107 9 0.06848082652931708 1107 10-11 5.548195000741885E-4 1107 12-13 0.01739615504390457 1107 14-15 -0.00775183252948608 1107 16-17 -0.004266556778226516 1107 18-19 -0.006497589969896467 1107 20-21 0.0059783120384793165 1107 22-23 -0.011669329760273683 1107 24-25 -0.03740260877961532 1107 26-27 0.10092827025147244 1107 28-29 0.018212321241954044 1107 30-31 0.04319940057859384 1107 32-33 -0.016495102331539613 1107 34-35 0.06639777347124465 1107 36-37 0.0390923742867173 1107 38-39 0.08343971605664535 1107 40-41 0.13104311614036845 1107 42-43 0.10745601643789371 1107 44-45 0.128254872475992 1107 46-47 0.21429352019492143 1107 48-49 0.23362752934236397 1107 50-51 0.14516077303051844 1107 52-53 0.2748657411717659 1107 54-55 0.24522791389023268 1107 56-57 0.29416877075330206 1107 58-59 0.303752836277873 1107 60-61 0.3260773222044264 1107 62-63 0.2566062430027216 1107 64-65 0.2816247742893978 1107 66-67 0.3301532947209722 1107 68-69 0.3198713559704629 1107 70-71 0.2738766639971928 1107 72-73 0.32652127094500116 1107 74-75 0.31185533119902686 1107 76-77 0.2693074304932459 1107 78-79 0.5460916344726776 1107 80-81 0.4290251821721647 1107 82-83 0.5493957711955595 1107 84-85 0.5610365191564917 1107 86-87 0.6692096952204771 1107 88-89 0.6847407576874396 1107 90-91 0.7532669904978455 1107 92-93 0.9285953476857252 1107 94-95 0.9253922268007173 1107 96-97 1.0408308220891698 1107 98-99 1.2112047250685407 1107 100-101 1.7760198540998395 1108 1 0.07267323518500035 1108 2 0.10406130345552356 1108 3 0.1123475735189885 1108 4 0.11268005048183483 1108 5 0.03916903038100372 1108 6 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2316 88-89 0.3492498687688652 2316 90-91 0.6050791244826819 2316 92-93 0.7121322413899165 2316 94-95 0.8886739915067636 2316 96-97 1.208580449781433 2316 98-99 1.3275515800289028 2316 100-101 1.8309399458416848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 17.0 5 43.0 6 78.0 7 223.0 8 746.0 9 1473.0 10 2474.0 11 3640.0 12 5235.0 13 6711.0 14 8198.0 15 9794.0 16 11505.0 17 13563.0 18 14876.0 19 15726.0 20 17301.0 21 19562.0 22 22289.0 23 26113.0 24 29446.0 25 32841.0 26 38005.0 27 46223.0 28 56837.0 29 71306.0 30 89934.0 31 114202.0 32 147155.0 33 197810.0 34 278637.0 35 423080.0 36 677096.0 37 1085085.0 38 480712.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.30201201750074 23.913346027007563 13.808211274385911 24.976430681105786 2 13.523743280669553 21.49192819137484 41.823301176462245 23.161027351493363 3 17.282261349198468 29.211609891123075 30.798967766513407 22.707160993165047 4 10.944329504167232 19.52874711495401 41.67533784673744 27.851585534141314 5 12.122588364981874 39.94076410405011 35.61912225705329 12.317525273914725 6 26.65706504448898 41.66548461680308 19.42894833361365 12.248502005094297 7 25.05205241006458 31.662455191866957 26.71714695041267 16.56834544765579 8 23.952467410885262 37.916672576915985 20.42607493925952 17.70478507293923 9 26.371372035672238 15.157661810805829 23.159700340227722 35.31126581329421 10-11 24.10591436960605 26.905422164042854 30.00433896732071 18.98432449903038 12-13 24.80334067555163 23.94355137762846 28.68481670459358 22.568291242226334 14-15 22.08734159917253 26.029781894866318 25.80670327567408 26.07617323028707 16-17 21.504851639232978 27.07513075160134 28.684373435894734 22.73564417327095 18-19 20.488563160976955 29.2684496031352 28.801434366850188 21.441552869037654 20-21 21.128541843551986 28.19519476466738 27.70288788290817 22.973375508872465 22-23 21.774624969351137 28.53458661014442 27.55676119012833 22.134027230376116 24-25 21.483663395428103 28.178971130289586 29.077514777311936 21.25985069697037 26-27 22.312157923212688 27.481785455923387 27.52475719007159 22.681299430792333 28-29 21.167169544451493 28.612069978702838 28.336242864640433 21.88451761220524 30-31 22.779299807697374 28.53414334144557 27.55234116795983 21.134215682897224 32-33 21.852627595528205 28.628800205879312 27.571743672149353 21.94682852644313 34-35 22.575256893208213 27.63653689110079 28.264332016867517 21.52387419882349 36-37 22.41771919763806 27.579975805127937 28.765086333544755 21.23721866368925 38-39 23.09808502759159 26.778901513750707 29.509488741222643 20.61352471743506 40-41 22.28519501244356 26.033727432193675 30.117482411201102 21.56359514416166 42-43 22.442253922143294 27.247072609616602 28.659130322004078 21.651543146236023 44-45 21.382332170871724 27.882437035581052 28.638881402573084 22.096349390974137 46-47 22.118284859157068 26.65060598630578 28.107870830994564 23.123238323542584 48-49 22.723306331909807 25.537305531492372 28.558856010982943 23.180532125614878 50-51 21.35894024839258 26.79544086744898 28.279162521048928 23.566456363109506 52-53 20.272362019319424 26.855763404952043 29.173045514323405 23.698829061405128 54-55 21.1861541095821 26.809410163872638 28.112974753441282 23.891460973103975 56-57 22.125503806538287 26.109894162632504 28.159024038841483 23.60557799198773 58-59 20.330341565128585 26.831598928454913 29.676978700812374 23.161080805604133 60-61 21.240587505800487 28.35668388406725 26.576440800578734 23.826287809553527 62-63 21.733135019139077 27.136061200462823 26.053864999098263 25.076938781299834 64-65 20.28345640161056 27.335747416883322 26.70805360967633 25.672742571829794 66-67 21.021625334217024 26.921208815996266 26.48137408584655 25.575791763940163 68-69 20.470229123655844 27.1830456094132 26.573252441325852 25.773472825605108 70-71 20.439672169682282 26.860054064240803 26.78419087027622 25.916082895800702 72-73 20.639350507504666 26.855218817693462 26.868618197218602 25.63681247758327 74-75 20.762615053615278 27.656472663478844 26.36279864293852 25.218113639967356 76-77 20.56350450642252 27.156776237690977 26.57190322213358 25.707816033752923 78-79 20.70569099965841 26.747436218782244 26.422122972586674 26.12474980897267 80-81 20.58584451833706 27.546139327737095 26.77016979961604 25.097846354309812 82-83 21.168179230413138 26.1877250876603 26.930967945807343 25.713127736119212 84-85 21.567779136603903 25.737249015413955 26.717566919935194 25.977404928046948 86-87 20.525921178946945 26.99195527782914 26.48289103403556 25.999232509188356 88-89 21.485841744462434 25.090072199605668 27.123054430356664 26.301031625575238 90-91 21.363286987263116 24.89783605059191 26.891190312120727 26.84768665002425 92-93 20.41643605912503 25.739689784835246 27.36469089301794 26.479183263021778 94-95 19.98969044745231 24.944696976413418 27.529266337284486 27.53634623884979 96-97 19.821694043372283 24.91518739071503 27.73497075981915 27.52814780609354 98-99 19.840529652902877 25.555327025915258 27.58526969987416 27.018873621307698 100-101 18.685700578732785 25.237529179804337 27.270350887147103 28.806419354315775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40.0 1 26.0 2 19.5 3 29.0 4 26.5 5 21.0 6 34.5 7 60.0 8 94.5 9 116.5 10 108.0 11 108.0 12 113.5 13 136.5 14 173.5 15 199.0 16 224.5 17 267.0 18 296.0 19 326.5 20 391.0 21 463.0 22 513.0 23 546.5 24 600.5 25 652.0 26 717.0 27 816.0 28 1025.5 29 1426.5 30 2143.0 31 3328.5 32 6856.0 33 15067.0 34 30815.5 35 52568.0 36 88415.0 37 159331.0 38 226524.0 39 237345.0 40 225102.0 41 208225.0 42 192774.5 43 195615.0 44 205044.0 45 217175.0 46 220860.0 47 205704.5 48 194864.0 49 174923.0 50 144508.0 51 130752.5 52 118921.0 53 108587.5 54 102268.5 55 90306.5 56 77076.5 57 62763.0 58 50009.5 59 47331.0 60 40062.5 61 28443.5 62 20580.5 63 16537.0 64 12638.5 65 7129.0 66 4276.0 67 3261.5 68 2812.0 69 2313.0 70 1670.0 71 1105.5 72 700.0 73 474.0 74 331.0 75 233.5 76 181.5 77 126.5 78 82.5 79 55.5 80 39.5 81 33.5 82 27.5 83 21.5 84 13.0 85 8.5 86 9.0 87 8.0 88 5.5 89 3.5 90 2.5 91 0.5 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08097885886932539 2 1.5197783960461446E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.1530193943987045E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.0659279868204814E-5 40-41 0.0030142271521581866 42-43 0.005825817184843553 44-45 0.0 46-47 0.0 48-49 1.0131855973640963E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002469639893574985 68-69 0.007446914140626109 70-71 0.0012158227168369156 72-73 0.0 74-75 5.0659279868204814E-5 76-77 0.001988376734827039 78-79 0.005331889206128557 80-81 0.004382027708599717 82-83 0.004470681448369075 84-85 0.0023303268739374217 86-87 3.546149590774337E-4 88-89 0.0 90-91 1.2664819967051204E-5 92-93 9.87855957429994E-4 94-95 0.003774116350181259 96-97 0.003115545711894596 98-99 0.0 100-101 2.0263711947281926E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3947944.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.944401434119072 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89461080710221 16.954676141761468 2 13.406672980430528 6.152161735179113 3 4.605741306091586 3.170279322860077 4 2.179149526172326 1.9999712605387285 5 1.2159375650401618 1.3949479805553369 6 0.7731510865250049 1.0643693339073024 7 0.5171673525463408 0.8306266741810678 8 0.3680047921792727 0.6754919745152637 9 0.28604615383994114 0.5906842004150451 >10 1.979151814874846 9.227423340950672 >50 0.31545096585966836 5.09322381636068 >100 0.3586842019046928 17.858202619683254 >500 0.06238103372934069 9.813466073487334 >1k 0.03318848591649976 13.778180020317274 >5k 0.003107951858401315 5.1299973040626625 >10k+ 0.0015539759292006576 6.266298201224772 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 32187 0.8152851205589543 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 28645 0.7255675359123634 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 22588 0.5721459068315052 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 21977 0.5566694968317686 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 21448 0.5432701173066284 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 16449 0.4166472472760505 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 16198 0.41028950765259087 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 13783 0.3491184272117335 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 13639 0.3454709590612227 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 12711 0.32196505320237573 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 12319 0.3120358343482076 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 11797 0.2988137623026061 No Hit CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC 11503 0.29136684816198 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10803 0.2736361002081083 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 9738 0.24666003367828926 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9541 0.24167009461127106 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 9109 0.23072769015973885 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 8961 0.22697890344949168 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 8495 0.21517529124019996 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 8317 0.21066661533192974 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 8187 0.20737376214049644 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 8049 0.2038782718295903 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 7917 0.2005347593582888 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 7810 0.19782448788533982 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7686 0.19468361253351113 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 7549 0.1912134518625391 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 7515 0.1903522441047796 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 7482 0.18951636598695423 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6991 0.17707951277930994 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 6704 0.16980990611822255 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 6659 0.16867007232118794 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6557 0.1660864490479095 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 6545 0.16578249336870027 RNA PCR Primer, Index 27 (95% over 23bp) ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 6459 0.16360414433436746 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 6355 0.1609698617812208 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 6267 0.1587408534670198 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 5956 0.15086333544751396 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5721 0.14491087006299988 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 5503 0.13938900855736555 No Hit CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA 5189 0.1314355016180574 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 5161 0.13072627169990253 No Hit GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA 5007 0.12682550715005075 No Hit ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC 4942 0.1251790805543341 No Hit CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT 4819 0.12206353484243951 No Hit GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG 4791 0.12135430492428464 No Hit ATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATA 4440 0.1124636013074147 No Hit ATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCT 4208 0.10658712484270293 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 4081 0.10337026057107193 No Hit GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC 4009 0.10154652649581655 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.0659279868204814E-5 0.0 2 0.0 0.0 0.0 5.0659279868204814E-5 0.0 3 0.0 0.0 0.0 5.0659279868204814E-5 0.0 4 0.0 0.0 0.0 5.0659279868204814E-5 0.0 5 0.0 0.0 0.0 5.0659279868204814E-5 0.0 6 0.0 0.0 0.0 7.598891980230723E-5 0.0 7 0.0 0.0 0.0 1.0131855973640963E-4 0.0 8 0.0 0.0 0.0 1.0131855973640963E-4 0.0 9 0.0 0.0 0.0 1.7730747953871687E-4 0.0 10-11 0.0 0.0 0.0 2.532963993410241E-4 0.0 12-13 0.0 0.0 0.0 3.4195013911038255E-4 0.0 14-15 0.0 0.0 0.0 3.5461495907743374E-4 0.0 16-17 0.0 0.0 0.0 4.6859833878089453E-4 0.0 18-19 0.0 0.0 0.0 6.839002782207651E-4 0.0 20-21 0.0 0.0 0.0 0.0010385152372981987 0.0 22-23 0.0 0.0 0.0 0.0019630470948929367 0.0 24-25 0.0 0.0 0.0 0.004040077569489334 0.0 26-27 0.0 0.0 0.0 0.009941883674135194 0.0 28-29 0.0 0.0 0.0 0.022454725801581785 0.0 30-31 0.0 0.0 0.0 0.08400575084145064 0.0 32-33 0.0 0.0 0.0 0.2742440115665268 0.0 34-35 0.0 0.0 0.0 0.5780097184762499 0.0 36-37 0.0 0.0 0.0 1.065060699949138 0.0 38-39 0.0 0.0 0.0 1.7554453659930334 0.0 40-41 0.0 0.0 0.0 2.4393203145738642 0.0 42-43 0.0 0.0 0.0 3.1881404599457337 0.0 44-45 0.0 0.0 0.0 3.969496527812958 0.0 46-47 0.0 0.0 0.0 4.880122413083874 0.0 48-49 0.0 0.0 0.0 5.763683578085201 0.0 50-51 0.0 0.0 0.0 6.629995258291404 0.0 52-53 0.0 0.0 0.0 7.440607060282517 0.0 54-55 0.0 0.0 0.0 8.229384712650432 0.0 56-57 0.0 0.0 0.0 9.162401999623096 0.0 58-59 0.0 0.0 0.0 10.257883597133091 0.0 60-61 0.0 0.0 0.0 11.26804230252506 0.0 62-63 0.0 0.0 0.0 12.234887323629717 0.0 64-65 0.0 0.0 0.0 13.203670062189332 0.0 66-67 0.0 0.0 0.0 14.13585147104417 0.0 68-69 0.0 0.0 0.0 15.059965389579993 0.0 70-71 0.0 0.0 0.0 15.974985967379478 0.0 72-73 0.0 0.0 0.0 16.898555298656717 0.0 74-75 0.0 0.0 0.0 17.783104319615475 0.0 76-77 0.0 0.0 0.0 18.694908033143328 0.0 78-79 0.0 0.0 0.0 19.69997548090854 0.0 80-81 0.0 0.0 0.0 20.734450640637252 1.2664819967051204E-5 82-83 0.0 0.0 0.0 21.757907406994626 2.5329639934102407E-5 84-85 0.0 0.0 0.0 22.70531192944986 2.5329639934102407E-5 86-87 0.0 0.0 0.0 23.67424664584908 2.5329639934102407E-5 88-89 0.0 0.0 0.0 24.63919194395868 2.5329639934102407E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3060 0.0 51.067802 3 TACCTGG 3120 0.0 50.84691 2 GTACCCG 255 0.0 50.327393 1 CTGGGGG 2550 0.0 48.80157 5 CCTGGGG 3055 0.0 44.465946 4 ATTAATA 3255 0.0 43.922615 2 ATAATGA 3245 0.0 40.545044 4 TATTCGC 1535 0.0 34.656315 9 CCGTGTT 1060 0.0 34.503056 9 GTACTAG 3015 0.0 34.367664 1 TATAATG 4410 0.0 33.71153 3 GTTATAA 4720 0.0 33.634525 1 TTAATAG 4430 0.0 33.452114 3 GAACCGT 1060 0.0 33.158783 6 ATAGCAT 4380 0.0 33.07489 6 TGAACCG 1120 0.0 32.65468 5 GTACCTG 5085 0.0 31.687614 1 ACCGTGT 1110 0.0 31.665142 8 TTATAAT 4980 0.0 31.474388 2 AACCGTG 1130 0.0 31.104696 7 >>END_MODULE