##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931101_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4950185 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21321970795031 33.0 33.0 33.0 27.0 33.0 2 31.149906720657913 33.0 33.0 33.0 27.0 33.0 3 31.167581413623935 33.0 33.0 33.0 27.0 33.0 4 34.79100558868002 37.0 37.0 37.0 33.0 37.0 5 34.780506587127554 37.0 37.0 37.0 33.0 37.0 6 34.874295405121224 37.0 37.0 37.0 33.0 37.0 7 34.8396613459901 37.0 37.0 37.0 33.0 37.0 8 34.8418222753291 37.0 37.0 37.0 33.0 37.0 9 34.8562267874837 37.0 37.0 37.0 33.0 37.0 10-11 34.76639398325517 37.0 37.0 37.0 33.0 37.0 12-13 34.690550555181275 37.0 37.0 37.0 33.0 37.0 14-15 36.37414187550566 40.0 37.0 40.0 33.0 40.0 16-17 36.32473109590853 40.0 37.0 40.0 33.0 40.0 18-19 36.253798898425 40.0 37.0 40.0 33.0 40.0 20-21 36.17584474115614 40.0 37.0 40.0 33.0 40.0 22-23 36.098735198785505 40.0 37.0 40.0 33.0 40.0 24-25 35.98001549437041 40.0 37.0 40.0 33.0 40.0 26-27 35.808435644324405 40.0 37.0 40.0 27.0 40.0 28-29 35.71774267022344 40.0 37.0 40.0 27.0 40.0 30-31 35.60664651927151 40.0 37.0 40.0 27.0 40.0 32-33 35.45783955549136 37.0 37.0 40.0 27.0 40.0 34-35 35.368272498906606 37.0 37.0 40.0 27.0 40.0 36-37 35.18792822894498 37.0 37.0 40.0 27.0 40.0 38-39 35.03621844436117 37.0 37.0 40.0 27.0 40.0 40-41 34.8337460115127 37.0 37.0 40.0 27.0 40.0 42-43 34.68540903016756 37.0 37.0 40.0 27.0 40.0 44-45 34.47017858928505 37.0 35.0 40.0 22.0 40.0 46-47 34.37564252649143 37.0 37.0 40.0 22.0 40.0 48-49 34.41411573506849 37.0 37.0 40.0 22.0 40.0 50-51 33.64947613069007 37.0 35.0 38.5 22.0 40.0 52-53 33.7842072569005 37.0 33.0 38.5 22.0 40.0 54-55 34.317591867778674 37.0 35.0 40.0 22.0 40.0 56-57 34.30120773264029 37.0 33.0 40.0 22.0 40.0 58-59 34.17341250882542 37.0 33.0 40.0 22.0 40.0 60-61 34.214274517013 37.0 33.0 40.0 22.0 40.0 62-63 34.07336746000402 37.0 33.0 40.0 22.0 40.0 64-65 33.87025737421935 37.0 33.0 40.0 22.0 40.0 66-67 33.64890009161274 37.0 33.0 40.0 22.0 40.0 68-69 33.4236962860984 37.0 33.0 40.0 18.5 40.0 70-71 33.17092714716723 37.0 33.0 40.0 15.0 40.0 72-73 32.528711250185594 37.0 33.0 38.5 10.5 40.0 74-75 32.307519011915716 37.0 33.0 37.0 15.0 40.0 76-77 31.90558807398107 37.0 33.0 37.0 6.0 40.0 78-79 31.60490769536896 37.0 33.0 37.0 6.0 37.0 80-81 31.28697573929055 37.0 33.0 37.0 4.0 37.0 82-83 30.984568051497064 37.0 33.0 37.0 2.0 37.0 84-85 30.569574167430105 37.0 33.0 37.0 2.0 37.0 86-87 30.369262259895336 37.0 33.0 37.0 2.0 37.0 88-89 30.122035035054246 35.0 33.0 37.0 2.0 37.0 90-91 29.78442098628637 33.0 33.0 37.0 2.0 37.0 92-93 29.452128657817838 33.0 27.0 37.0 2.0 37.0 94-95 29.148840396874057 33.0 27.0 37.0 2.0 37.0 96-97 28.790679843278582 33.0 27.0 37.0 2.0 37.0 98-99 28.39892488866578 33.0 27.0 37.0 2.0 37.0 100-101 27.621092343013444 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08522517475032743 1101 2 0.12488904749752194 1101 3 0.08786600492515007 1101 4 0.14241333332335415 1101 5 0.12891721043635584 1101 6 0.13972760583106236 1101 7 0.1900081519637311 1101 8 0.13056751793858723 1101 9 0.20197645679859733 1101 10-11 0.1492039399236944 1101 12-13 0.19849001252599408 1101 14-15 0.36708601343645597 1101 16-17 0.2664217164276934 1101 18-19 0.22703045454488802 1101 20-21 0.3570125372920856 1101 22-23 0.36091579516119054 1101 24-25 0.3256253392058639 1101 26-27 0.42483520722458934 1101 28-29 0.37655091265438045 1101 30-31 0.3273056458128707 1101 32-33 0.329066114586162 1101 34-35 0.3485832015722323 1101 36-37 0.3158606668196384 1101 38-39 0.4350581651210632 1101 40-41 0.3930251803805973 1101 42-43 0.3580721389942809 1101 44-45 0.23087879906456266 1101 46-47 0.315757308053783 1101 48-49 0.3307025575475251 1101 50-51 0.31185879751888734 1101 52-53 0.43164845538280616 1101 54-55 0.4743807482637834 1101 56-57 0.40409011092670255 1101 58-59 0.4970194761060185 1101 60-61 0.4471416995743809 1101 62-63 0.4922745878073016 1101 64-65 0.5432648743709976 1101 66-67 0.5300799231920834 1101 68-69 0.44543028562367937 1101 70-71 0.3571929164641503 1101 72-73 0.33750704646205065 1101 74-75 0.28309095682072183 1101 76-77 0.16805132390429023 1101 78-79 0.10117318523739272 1101 80-81 0.24873160620817458 1101 82-83 0.050787687640628576 1101 84-85 0.13132318186974956 1101 86-87 0.12666972830067635 1101 88-89 -0.00549216166825417 1101 90-91 -0.09439159962472843 1101 92-93 -0.08313418749196799 1101 94-95 -0.20264633681837552 1101 96-97 -0.33336538807845884 1101 98-99 -0.2670601154034564 1101 100-101 -0.21235126198392962 1102 1 0.16299931907123266 1102 2 0.12895624828234986 1102 3 0.11870724102717745 1102 4 0.19217352516987773 1102 5 0.11689273910432973 1102 6 0.160663130138893 1102 7 0.21446585852575595 1102 8 0.1559381305394112 1102 9 0.09973779318403331 1102 10-11 0.11377432906692064 1102 12-13 0.13615351808518739 1102 14-15 0.33055614642098163 1102 16-17 0.3575726593097599 1102 18-19 0.36863648790002657 1102 20-21 0.31962085972504894 1102 22-23 0.39541546815006967 1102 24-25 0.27583789643281165 1102 26-27 0.12668282004342757 1102 28-29 0.27423049626022333 1102 30-31 0.3072012747642532 1102 32-33 0.37586958090402334 1102 34-35 0.24654551354083765 1102 36-37 0.3473968015482285 1102 38-39 0.37034402734037286 1102 40-41 0.2904015974287759 1102 42-43 0.3303545403459154 1102 44-45 0.2974289680203057 1102 46-47 0.2747412572580572 1102 48-49 0.28983979321353814 1102 50-51 0.2242504750859169 1102 52-53 0.31872061583626277 1102 54-55 0.36252932031523244 1102 56-57 0.3945216293483256 1102 58-59 0.39747184148644266 1102 60-61 0.39317123957874145 1102 62-63 0.3867487811798753 1102 64-65 0.40024860556786024 1102 66-67 0.40865660729934206 1102 68-69 0.43217270986610146 1102 70-71 0.41962138278200456 1102 72-73 0.28892886355382785 1102 74-75 0.29862398494368136 1102 76-77 0.3324425382055729 1102 78-79 0.29944070758315533 1102 80-81 0.33959982072746797 1102 82-83 0.3067862978433773 1102 84-85 0.36155604103695893 1102 86-87 0.21245564502184422 1102 88-89 0.3329881904137153 1102 90-91 0.29256133802515194 1102 92-93 0.22326160496497494 1102 94-95 0.16685919511971292 1102 96-97 0.24199364287859382 1102 98-99 0.25912802499331633 1102 100-101 0.22947482260294194 1103 1 0.1894638535010955 1103 2 0.15689977998861337 1103 3 0.18699545004909268 1103 4 0.1751144796924251 1103 5 0.13129527102906025 1103 6 0.202805965740815 1103 7 0.27486652714321025 1103 8 0.24708484262160368 1103 9 0.1665567197265858 1103 10-11 0.20240794746739255 1103 12-13 0.2293894640313212 1103 14-15 0.38825785104556587 1103 16-17 0.3147703685514003 1103 18-19 0.36848883219334994 1103 20-21 0.4267602686740375 1103 22-23 0.36975514395670217 1103 24-25 0.35359913756949624 1103 26-27 0.3404887667423395 1103 28-29 0.38370997421189657 1103 30-31 0.4647038951120521 1103 32-33 0.35287748787401796 1103 34-35 0.4718023743860371 1103 36-37 0.45708717347071115 1103 38-39 0.43378393107640534 1103 40-41 0.44570913406242596 1103 42-43 0.4351092658925424 1103 44-45 0.42953830598911935 1103 46-47 0.3045452877017496 1103 48-49 0.32568624324944295 1103 50-51 0.3180819472989569 1103 52-53 0.511361749161729 1103 54-55 0.46164543493050303 1103 56-57 0.5197812987367456 1103 58-59 0.5483684919303187 1103 60-61 0.5679086235220225 1103 62-63 0.467185965463365 1103 64-65 0.5934711533815786 1103 66-67 0.6394559440227283 1103 68-69 0.5809180622450398 1103 70-71 0.5123988915995668 1103 72-73 0.4727768163905637 1103 74-75 0.46589807965465724 1103 76-77 0.39327438726985164 1103 78-79 0.46916532531625066 1103 80-81 0.48890190924768717 1103 82-83 0.48726218016909684 1103 84-85 0.5741664231162424 1103 86-87 0.5726141343386786 1103 88-89 0.5057305417951632 1103 90-91 0.5651869967378857 1103 92-93 0.43641191796790935 1103 94-95 0.40883646479672464 1103 96-97 0.4191705689004124 1103 98-99 0.45957283378102076 1103 100-101 0.5489667110927279 1104 1 0.028726363918384124 1104 2 0.07880036266111645 1104 3 0.09268219108696485 1104 4 0.09105178773067735 1104 5 0.02354172284783118 1104 6 -0.011488051943253197 1104 7 0.08667474211689097 1104 8 0.031793634616263944 1104 9 0.10454881019623485 1104 10-11 0.034877343358203916 1104 12-13 0.005232617618538882 1104 14-15 0.05808132340502681 1104 16-17 0.061466963679876585 1104 18-19 0.13375439095771924 1104 20-21 0.1561084345142021 1104 22-23 0.12128288230662321 1104 24-25 0.11811958588345561 1104 26-27 0.1877357554562451 1104 28-29 0.1016577045366418 1104 30-31 0.009855992937389146 1104 32-33 0.09985563776383799 1104 34-35 0.06097006955828732 1104 36-37 0.08355093000637481 1104 38-39 0.1018424096069026 1104 40-41 0.09059576224363752 1104 42-43 0.08168585300453657 1104 44-45 0.08434664747007048 1104 46-47 3.7504716838299146E-4 1104 48-49 -0.009061674706025258 1104 50-51 -0.04195623094414458 1104 52-53 -0.03541840162972676 1104 54-55 0.07355392104709324 1104 56-57 0.05965023981000428 1104 58-59 0.0021178675451949402 1104 60-61 -0.01984019041795193 1104 62-63 0.01888911853760078 1104 64-65 -0.09171814888516394 1104 66-67 -0.031616407269275726 1104 68-69 0.040335485383181435 1104 70-71 0.09015189201734586 1104 72-73 -0.07346385261119792 1104 74-75 -0.09497711157347055 1104 76-77 -0.13359214929574748 1104 78-79 -0.04811141651718387 1104 80-81 0.01605395664400433 1104 82-83 0.02566174578398872 1104 84-85 0.12401939645016213 1104 86-87 0.009924672307594307 1104 88-89 -0.10459540788972177 1104 90-91 -0.0018330353193150017 1104 92-93 -0.04314100706718449 1104 94-95 0.03752335842584387 1104 96-97 0.07103611644867058 1104 98-99 -0.023306949965448354 1104 100-101 0.04692374904882257 1105 1 0.09775890902867701 1105 2 0.11146544330056685 1105 3 0.1263205157006695 1105 4 0.11208778402916408 1105 5 0.0982092462649291 1105 6 0.1520643466927325 1105 7 0.1384254145486139 1105 8 0.09723073853375297 1105 9 0.16614072022062487 1105 10-11 0.11823754290311683 1105 12-13 0.15941813563936336 1105 14-15 0.22988592972213695 1105 16-17 0.19672645871438021 1105 18-19 0.243060878253182 1105 20-21 0.2932851669952541 1105 22-23 0.2525997642286768 1105 24-25 0.18843849344914787 1105 26-27 0.22790248702782634 1105 28-29 0.23051719386533875 1105 30-31 0.17080709640484315 1105 32-33 0.1412177851984069 1105 34-35 0.11792496079052484 1105 36-37 0.25094266831238343 1105 38-39 0.21475932408174003 1105 40-41 0.28691801974419917 1105 42-43 0.2245043662347186 1105 44-45 0.14615409184856532 1105 46-47 0.18823036655871306 1105 48-49 0.22010104276495923 1105 50-51 0.24144963566350697 1105 52-53 0.282264497698165 1105 54-55 0.3652545002936023 1105 56-57 0.3323689954297535 1105 58-59 0.2682155174704022 1105 60-61 0.3116014936457887 1105 62-63 0.27572746950355054 1105 64-65 0.338402505003522 1105 66-67 0.345075638416418 1105 68-69 0.2936064913044376 1105 70-71 0.31041499481989376 1105 72-73 0.2676858030161853 1105 74-75 0.21343768684707953 1105 76-77 0.23318432665490363 1105 78-79 0.26636914032991754 1105 80-81 0.22186854828679614 1105 82-83 0.26590290019720086 1105 84-85 0.22527785240129816 1105 86-87 0.26747765075902663 1105 88-89 0.16882542161740943 1105 90-91 0.24020194374606518 1105 92-93 0.29968656371799796 1105 94-95 0.29868635178740277 1105 96-97 0.14996980187092035 1105 98-99 0.19008150156533432 1105 100-101 0.1099959323615316 1106 1 0.22811737829909617 1106 2 0.13023738593471634 1106 3 0.16130966344029218 1106 4 0.10156804621291826 1106 5 0.15323377551423079 1106 6 0.1591087506097395 1106 7 0.15430744572628186 1106 8 0.17169229632362004 1106 9 0.16210252091674704 1106 10-11 0.13163046815120794 1106 12-13 0.13792117635257028 1106 14-15 0.26376233836429464 1106 16-17 0.27933184148447054 1106 18-19 0.2823662927989332 1106 20-21 0.3423255546989381 1106 22-23 0.32140898920066974 1106 24-25 0.3681721164480223 1106 26-27 0.2961128083615989 1106 28-29 0.27989043044951245 1106 30-31 0.32545937334000996 1106 32-33 0.31326606623380826 1106 34-35 0.29889726295041896 1106 36-37 0.4654418877899147 1106 38-39 0.3969194194984169 1106 40-41 0.4882254305674181 1106 42-43 0.424434329282775 1106 44-45 0.4315721700297033 1106 46-47 0.28216052458443386 1106 48-49 0.33147674471511124 1106 50-51 0.3459420350022171 1106 52-53 0.3917731458544651 1106 54-55 0.5195801284744803 1106 56-57 0.5281681651839065 1106 58-59 0.5618999139146297 1106 60-61 0.5205825192200919 1106 62-63 0.5643428140816837 1106 64-65 0.5283449213313887 1106 66-67 0.6517211743459441 1106 68-69 0.616379493670685 1106 70-71 0.5985342273258496 1106 72-73 0.4264701173495311 1106 74-75 0.5620540185505618 1106 76-77 0.5633096793380652 1106 78-79 0.585193967593181 1106 80-81 0.7033842384811742 1106 82-83 0.757747817093076 1106 84-85 0.7955323186336578 1106 86-87 0.77040370242484 1106 88-89 0.7053900948483225 1106 90-91 0.7710567494353313 1106 92-93 0.8767826244759718 1106 94-95 0.8423439286067627 1106 96-97 0.8269371896149842 1106 98-99 0.768715866662216 1106 100-101 0.80345510514233 1107 1 0.09874631659973332 1107 2 0.13927264371333337 1107 3 0.11790151260148463 1107 4 0.18724688229173836 1107 5 0.19693230008284957 1107 6 0.1613154043207956 1107 7 0.13424537387005842 1107 8 0.0638336198337015 1107 9 0.10918840201905056 1107 10-11 0.07984452195977809 1107 12-13 0.17740018335840801 1107 14-15 0.25052719863263206 1107 16-17 0.2114298422294567 1107 18-19 0.2970363484551868 1107 20-21 0.32778252782886597 1107 22-23 0.15171614457238292 1107 24-25 0.2633600311773776 1107 26-27 0.23661636648961348 1107 28-29 0.1339682605629946 1107 30-31 0.1898674005328047 1107 32-33 0.2005663370885813 1107 34-35 0.22177189288721877 1107 36-37 0.27326716430206943 1107 38-39 0.2615432828687858 1107 40-41 0.24099607342561313 1107 42-43 0.22506158008744848 1107 44-45 0.16620180354757252 1107 46-47 0.0927596323112212 1107 48-49 0.09481892768166489 1107 50-51 0.1405876958435286 1107 52-53 0.18441737426832105 1107 54-55 0.2842615377902362 1107 56-57 0.22310349987190392 1107 58-59 0.20636986521054013 1107 60-61 0.3253616322193267 1107 62-63 0.2593783875853575 1107 64-65 0.2851787745223504 1107 66-67 0.3030432535587195 1107 68-69 0.3889965666195039 1107 70-71 0.44084270625431543 1107 72-73 0.271319833002579 1107 74-75 0.31876963037672823 1107 76-77 0.3233035825784434 1107 78-79 0.3214409143530723 1107 80-81 0.33872129830329456 1107 82-83 0.35798982112284605 1107 84-85 0.41562662399577377 1107 86-87 0.45947152809795355 1107 88-89 0.4536290722724239 1107 90-91 0.5634875523801313 1107 92-93 0.6726544805426755 1107 94-95 0.6986323395532423 1107 96-97 0.7309629173184611 1107 98-99 0.6314283103507847 1107 100-101 0.6712599761733067 1108 1 -0.10018343007769204 1108 2 -0.13895646636267145 1108 3 -0.09868302058783485 1108 4 -0.0308552361054808 1108 5 -0.09595260839520137 1108 6 -0.12697356550501837 1108 7 -0.12540235405614908 1108 8 -0.06569689242274279 1108 9 -0.08356838609229555 1108 10-11 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-1.2662122206342659 2316 66-67 -1.2707899956758482 2316 68-69 -1.271469354234675 2316 70-71 -1.2121590880046895 2316 72-73 -0.9492423691666083 2316 74-75 -0.9345657176805062 2316 76-77 -1.0427859553371839 2316 78-79 -1.0084983841127766 2316 80-81 -0.9454577100666199 2316 82-83 -1.0606235763048382 2316 84-85 -0.9686159028751895 2316 86-87 -0.8958348307261979 2316 88-89 -0.9343466954308219 2316 90-91 -0.9206577405122545 2316 92-93 -0.9656646334893288 2316 94-95 -0.9157871502718677 2316 96-97 -0.8633141956460157 2316 98-99 -0.749355417243958 2316 100-101 -0.8473660018832199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134256.0 3 20047.0 4 14870.0 5 12849.0 6 13136.0 7 13622.0 8 13862.0 9 14191.0 10 14627.0 11 14612.0 12 14375.0 13 14751.0 14 15626.0 15 17030.0 16 19448.0 17 23217.0 18 21286.0 19 18170.0 20 19435.0 21 21552.0 22 24088.0 23 28153.0 24 33065.0 25 38366.0 26 44309.0 27 52413.0 28 62541.0 29 75982.0 30 93460.0 31 117604.0 32 151395.0 33 199375.0 34 273693.0 35 393006.0 36 610313.0 37 1134884.0 38 1166167.0 39 409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.68531065069034 24.658606856589223 13.537769905526249 23.118312587194186 2 13.689000726866043 21.920122310666347 44.082870001684846 20.30800696078277 3 16.870750278222374 31.429361010727497 30.077550762326275 21.622337948723853 4 10.617970900193448 19.096243012313195 43.96321636415646 26.32256972333689 5 11.69608222214411 39.173960455764345 37.02988342462438 12.100073897467164 6 24.76788241247549 42.7423217516113 20.963438740168296 11.526357095744906 7 23.323512151566053 32.40779890044514 28.49028874678421 15.778400201204601 8 22.022065841983686 38.858527509577925 22.184989045863944 16.93441760257445 9 25.318124474135818 15.849650063583482 24.406481777953754 34.425743684326946 10-11 22.17507918394128 28.233430966087514 31.200624300152136 18.390865549819065 12-13 22.588985355590424 25.7245768211576 30.59985162163213 21.086586201619838 14-15 20.594857083694233 27.435940818331268 27.49705177512178 24.47215032285272 16-17 19.385909595641106 29.29222925828967 29.701019490718277 21.620841655350944 18-19 18.839634605506976 30.840844708350566 30.580349364165706 19.73917132197675 20-21 19.556968028161375 29.14175219125671 29.918602023823553 21.38267775675836 22-23 19.88623657499669 30.139580641935602 29.182384092715726 20.791798690351975 24-25 19.86180515089892 29.647854539563124 29.885976977221755 20.6043633323162 26-27 20.32548278498682 29.07671127442712 29.188393969114284 21.409411971471773 28-29 19.883701316213433 30.462225149161092 28.801903363207636 20.852170171417836 30-31 21.212914264820405 29.717889331408827 28.49532896245292 20.57386744131785 32-33 20.86325056538291 29.823743961084283 28.70486658579427 20.60813888773854 34-35 21.381473621692926 28.776015441847125 29.436637216588874 20.405873719871074 36-37 21.417936905388384 29.300682701757612 29.327065554115656 19.954314838738348 38-39 22.073690175215674 28.358566397013448 30.245495875406675 19.322247552364207 40-41 20.87708387771628 28.427057095842944 30.49519547434319 20.200663552097588 42-43 21.46205646859663 28.634333868330174 29.413769384376543 20.489840278696654 44-45 20.661514639440583 29.667707374819575 29.395746193806055 20.275031791933788 46-47 21.633268279845268 28.618999288947087 28.61821567546515 21.129516755742486 48-49 21.523631106331067 27.550283967693883 29.68877238237862 21.23731254359643 50-51 21.26755919361207 28.39514424393956 28.410935765829482 21.92636079661889 52-53 19.917269286425295 28.57325297704989 29.481259654779883 22.028218081744928 54-55 20.743244434742262 29.220899388053223 28.531929733243423 21.50392644396109 56-57 21.27043881409744 28.067318990476526 29.185695479424773 21.476546716001263 58-59 20.663329789104 27.648220633088176 30.448249608302717 21.240199969505106 60-61 20.74621114977581 29.657084087416525 28.30171957302179 21.294985189785876 62-63 21.3570647331681 28.492264294093854 27.587954870313986 22.562716102424055 64-65 20.046433898285173 28.481446037962403 28.303976748211255 23.16814331554117 66-67 20.877862521983374 27.10404182164858 29.140471512272764 22.877624144095282 68-69 19.78560926027713 28.084520722929646 28.58681899595219 23.54305102084103 70-71 19.943071206254128 27.766704746104786 29.289024960494526 23.00119908714656 72-73 20.58853993685682 27.671254166948017 29.465495058020778 22.274710838174382 74-75 20.970610184185006 28.422985691019786 28.271661410602288 22.334742714192917 76-77 20.118445334083972 28.424623381471253 29.447288654615566 22.00964262982921 78-79 20.22964091225451 27.950784658654683 28.86741420776815 22.95216022132266 80-81 20.29819682291243 28.360218390195524 29.603752111099478 21.737832675792568 82-83 21.228890523827715 27.241545193447 29.688341559182607 21.841222723542682 84-85 21.900659192843282 27.01644335681902 28.85804057256996 22.224856877767742 86-87 20.848085484369435 28.220954379956005 28.814783731204297 22.116176404470263 88-89 21.762597012951463 26.665778236754896 29.21703367227002 22.354591078023624 90-91 21.550771534248387 26.333966880989895 29.675399915353683 22.439861669408035 92-93 20.886021488830814 27.4638883332348 29.112196818707126 22.537893359227265 94-95 20.3009291680396 26.67293965062893 29.66450127955919 23.361629901772275 96-97 20.887831224427682 26.5652518583729 29.021974961796726 23.524941955402692 98-99 20.227731362090783 27.182160001327254 29.421628662544354 23.16847997403761 100-101 20.99151007937191 25.79458149102821 28.920770418290974 24.2931380113089 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45.0 1 29.5 2 18.5 3 27.0 4 22.0 5 23.0 6 36.0 7 58.5 8 93.0 9 131.5 10 130.0 11 144.0 12 233.5 13 417.5 14 590.0 15 741.0 16 1042.0 17 1276.5 18 1337.5 19 1398.0 20 1527.0 21 1662.5 22 1906.0 23 2170.0 24 2340.0 25 2644.5 26 3171.5 27 4375.0 28 8003.5 29 18058.5 30 33541.5 31 53715.0 32 84437.0 33 131318.5 34 197963.5 35 253507.0 36 305514.5 37 374022.0 38 392498.5 39 339437.5 40 279161.5 41 231349.5 42 194264.0 43 171165.5 44 149932.0 45 134960.0 46 128059.0 47 127792.0 48 128596.5 49 123219.0 50 115240.5 51 112919.5 52 109791.5 53 104780.0 54 99359.5 55 88332.5 56 78681.0 57 65857.0 58 52376.0 59 47790.5 60 41217.0 61 31610.0 62 24416.0 63 20394.5 64 16383.0 65 10960.0 66 7796.0 67 6072.0 68 4818.5 69 3874.5 70 3154.5 71 2599.0 72 2109.0 73 1788.5 74 1544.0 75 1289.0 76 1079.0 77 922.5 78 797.5 79 684.5 80 569.5 81 480.0 82 431.5 83 363.5 84 294.0 85 243.0 86 184.0 87 163.5 88 157.5 89 121.0 90 96.5 91 97.0 92 78.0 93 53.0 94 43.5 95 34.0 96 20.5 97 14.0 98 11.5 99 6.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.454341605414747E-4 2 0.003373611289275047 3 1.6161012164191843E-4 4 9.898619950567504E-4 5 0.0016767050120349038 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.040253041047961E-5 12-13 5.0503163013099514E-5 14-15 0.003484718247903866 16-17 0.004312970121318698 18-19 0.0038887435520086624 20-21 1.0100632602619902E-5 22-23 0.0 24-25 1.0100632602619902E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.7171075424453835E-4 42-43 0.0 44-45 6.060379561571941E-4 46-47 0.7484265739563268 48-49 0.5347981944109159 50-51 0.4105806146638964 52-53 0.3448658989512513 54-55 0.33505818469410736 56-57 0.30112005914930456 58-59 0.3016553926772434 60-61 0.2773027674723268 62-63 0.2713938973997942 64-65 0.2681616949669558 66-67 0.25547530041806515 68-69 0.23404175803530572 70-71 0.24770791394665048 72-73 0.8255550045099324 74-75 0.5450907390329857 76-77 0.6406528240863726 78-79 0.35671394099412446 80-81 0.3082006026037411 82-83 0.28727209185111263 84-85 0.2796865167665451 86-87 0.2522531986178294 88-89 0.2552530865008075 90-91 0.2660809646508161 92-93 0.22344619443515748 94-95 0.1903161194985642 96-97 0.14582283288402353 98-99 0.15455988008528976 100-101 0.150327715024792 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4950185.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.46977410766746 #Duplication Level Percentage of deduplicated Percentage of total 1 79.85729344812029 25.929482791101787 2 10.81511420978185 7.023286306804826 3 3.7166669230793996 3.620380062774828 4 1.6940323918041096 2.2001939637180423 5 0.9240081326704157 1.5001167670728015 6 0.5492236220412444 1.0699900165364482 7 0.3520019774282984 0.8000597284784379 8 0.26153193498165406 0.6793506280636386 9 0.19595372056570826 0.5726315738092985 >10 1.2062548861310671 7.679747752497799 >50 0.17379168457992253 3.9989843973203585 >100 0.1980819074850592 13.820978054814544 >500 0.03272435594694868 7.194721735283954 >1k 0.019872836846056646 12.180494641200399 >5k 0.002319542470990839 5.076781948555155 >10k+ 0.001128426066968516 6.652799631967741 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 30216 0.6104014294415259 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 28809 0.5819782492977535 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 25533 0.5157989044853879 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 23482 0.4743661095494411 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 21304 0.4303677539324288 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 18784 0.3794605656152245 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 18700 0.37776365933798434 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 18649 0.3767333948125171 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 18360 0.37089522916820283 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 17521 0.3539463676610066 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 16392 0.3311391392442909 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 14824 0.2994635554024749 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 13536 0.273444325818126 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13351 0.2697070917551566 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 13070 0.26403053623248424 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 12829 0.25916203131802146 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 11267 0.22760765506743685 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 10205 0.2061539114194722 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 9794 0.19785119142011867 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 9784 0.19764917876806623 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 9559 0.19310389409688727 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 9532 0.19255845993634582 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 8670 0.1751449693294291 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 8541 0.17253900611795317 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 8425 0.17019565935414535 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 8280 0.16726647589938556 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 7925 0.16009502675152543 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 7669 0.15492350285898404 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 7516 0.15183270928258238 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 7485 0.15120647006121993 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 7187 0.14518649303005848 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6776 0.13688377303070492 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 6758 0.1365201502570106 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 6679 0.13492425030579663 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 6597 0.13326774655896698 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6527 0.1318536579946002 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 6372 0.12872246188778805 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 6290 0.12706595814095836 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 6245 0.12615690120672257 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 6072 0.1226620823262161 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 5893 0.11904605585447815 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 5809 0.11734914957723802 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 5720 0.11555123697397167 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 5630 0.1137331231055001 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 5611 0.11334929906660055 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 5536 0.11183420417620756 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 5399 0.1090666308430897 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 5243 0.10591523347107229 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 5225 0.10555161069737796 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 5191 0.10486476768039983 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 5148 0.1039961132765745 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 5127 0.10357188670726447 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5084 0.10270323230343915 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 5062 0.1022588044689239 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 5046 0.10193558422564004 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 4994 0.10088511843496757 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.0201265205239803E-5 0.0 9 0.0 0.0 0.0 2.0201265205239803E-5 0.0 10-11 0.0 0.0 0.0 4.040253041047961E-5 0.0 12-13 0.0 0.0 0.0 4.040253041047961E-5 0.0 14-15 0.0 0.0 0.0 4.040253041047961E-5 0.0 16-17 0.0 0.0 0.0 4.040253041047961E-5 0.0 18-19 0.0 0.0 0.0 4.040253041047961E-5 1.0100632602619902E-5 20-21 0.0 0.0 0.0 1.8181138684715824E-4 2.0201265205239803E-5 22-23 0.0 0.0 0.0 4.4442783451527574E-4 3.0301897807859707E-5 24-25 0.0 0.0 0.0 0.0010403651580698499 4.040253041047961E-5 26-27 0.0 0.0 0.0 0.0028584790265414324 4.040253041047961E-5 28-29 0.0 0.0 0.0 0.007757285838812085 4.040253041047961E-5 30-31 0.0 0.0 0.0 0.03488758500944914 4.040253041047961E-5 32-33 0.0 0.0 0.0 0.12201564183964841 4.040253041047961E-5 34-35 0.0 0.0 0.0 0.2610306483495061 5.050316301309951E-5 36-37 0.0 0.0 0.0 0.48029518088717893 6.0603795615719414E-5 38-39 0.0 0.0 0.0 0.7739306712779421 6.0603795615719414E-5 40-41 2.0201265205239803E-5 0.0 0.0 1.0587887119370287 6.0603795615719414E-5 42-43 2.0201265205239803E-5 0.0 0.0 1.3827867039312673 6.0603795615719414E-5 44-45 3.0301897807859707E-5 0.0 0.0 1.7326221141230076 6.0603795615719414E-5 46-47 4.040253041047961E-5 0.0 0.0 2.1570910986155063 6.0603795615719414E-5 48-49 4.040253041047961E-5 0.0 0.0 2.57247961439825 6.0603795615719414E-5 50-51 4.040253041047961E-5 0.0 0.0 2.976161496994557 6.0603795615719414E-5 52-53 6.0603795615719414E-5 0.0 0.0 3.3807322352598943 6.0603795615719414E-5 54-55 6.0603795615719414E-5 0.0 0.0 3.797413632015773 6.0603795615719414E-5 56-57 6.0603795615719414E-5 0.0 0.0 4.294809183899188 6.0603795615719414E-5 58-59 6.0603795615719414E-5 0.0 0.0 4.8620910127601285 7.070442821833931E-5 60-61 6.0603795615719414E-5 0.0 0.0 5.421494348191027 1.0100632602619903E-4 62-63 6.0603795615719414E-5 0.0 0.0 5.95581781286962 1.0100632602619903E-4 64-65 6.0603795615719414E-5 0.0 0.0 6.510827373118378 1.0100632602619903E-4 66-67 6.0603795615719414E-5 0.0 0.0 7.099169020955783 1.0100632602619903E-4 68-69 6.0603795615719414E-5 0.0 0.0 7.717802465968443 1.0100632602619903E-4 70-71 6.0603795615719414E-5 0.0 0.0 8.333223909813473 1.0100632602619903E-4 72-73 6.0603795615719414E-5 0.0 0.0 9.016854925623992 1.0100632602619903E-4 74-75 6.0603795615719414E-5 0.0 0.0 9.638478157887029 1.0100632602619903E-4 76-77 6.0603795615719414E-5 0.0 0.0 10.320048644646615 1.0100632602619903E-4 78-79 6.0603795615719414E-5 0.0 0.0 11.068657029989788 1.0100632602619903E-4 80-81 6.0603795615719414E-5 0.0 0.0 11.890262687152095 1.0100632602619903E-4 82-83 6.0603795615719414E-5 0.0 0.0 12.76151295355628 1.0100632602619903E-4 84-85 6.0603795615719414E-5 0.0 0.0 13.56824037889493 1.2120759123143883E-4 86-87 6.0603795615719414E-5 0.0 0.0 14.43727860675914 1.2120759123143883E-4 88-89 6.0603795615719414E-5 3.0301897807859707E-5 0.0 15.321861708198785 1.4140885643667864E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3385 0.0 45.744072 3 TACCTGG 3440 0.0 44.049564 2 CCTGGGG 3210 0.0 40.41953 4 GTACCCG 445 0.0 37.244156 1 TATTCGC 1350 0.0 36.4792 9 CTGGGGG 3430 0.0 35.48009 5 GTACCTG 4600 0.0 33.559067 1 GTACACG 570 0.0 30.738094 1 TGGGGGG 8810 0.0 29.507565 6 GAACCGT 825 0.0 28.698088 6 GCATCGT 2450 0.0 27.831291 8 TATATCC 4080 0.0 27.506409 4 GTACTAG 2845 0.0 27.46324 1 CCGTGTT 855 0.0 27.137865 9 CATGGGG 8425 0.0 27.090862 4 TGAACCG 900 0.0 26.833258 5 ACCGTGT 900 0.0 26.832714 8 GTACATG 13155 0.0 26.60135 1 GGGGGGA 9115 0.0 26.026634 7 CTGTGCG 2930 0.0 25.858187 9 >>END_MODULE