FastQCFastQC Report
Sat 18 Jun 2016
SRR1931100_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931100_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316915
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC75680.3266412449312987No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA65770.2838688514684397No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT64300.2775242078367139No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA54550.23544238783036928No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC52110.22491114261852505No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC49760.21476834497597022No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC45720.19733136519898228No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC36810.1588750558393381No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT31810.13729463532326389No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT31380.13543871915888153No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG29580.12766976777309483No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC28910.12477799142394089No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT28370.12244730600820487No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT27850.12020294227453317No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT27150.11718168340228279No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT27150.11718168340228279No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC26410.1139877811659038No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC24500.10574406052876346No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC24400.10531245211844198No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG23930.10328389258993101No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC23680.1022048715641273No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG23180.10004682951251988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26500.050.5661162
GTACCTG28100.048.023921
ACCTGGG28550.047.596623
CCTGGGG29650.041.040134
CTGGGGG27250.039.615825
GTACCCG4000.037.8799171
CTGTGCG30550.031.6189489
TAGTACT29650.031.4587784
GTACTAG23500.030.827811
TGGGGGG71450.030.6154086
ACTGTGC34100.029.4362038
GTACTGT32750.029.0592946
TCTAACG10900.028.2361772
TACTGTG36300.028.0434977
CTAACGC11400.027.4119193
AGGGCTA23950.027.2818555
CTAGTAC36950.027.1657523
TATTCGC3150.027.0576739
GTACACG2800.027.0570851
GCATCGT25900.027.055928
Produced by FastQC (version 0.11.5)