FastQCFastQC Report
Sat 18 Jun 2016
SRR1931100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316915
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC99600.42988197668019756No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA72780.3141246010319757No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT67640.29193992874145147No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA56760.24498093369847404No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC56420.243513465103381No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC53860.232464289799151No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC52170.22517010766471796No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG38000.16401119592216373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.15071765688426203No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG32940.14217181035989665No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC32620.14079066344686791No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC32590.14066118092377147No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA32060.13837365634906762No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT31940.13785572625668185No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT31250.1348776282254636No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT29510.12736764188586977No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT29250.12624546001903394No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT28760.12413057880845865No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA28240.12188621507478695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.11946920797698662No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC26710.11528260639686826No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA26610.11485099798654677No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG25830.1114844523860392No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC25830.1114844523860392No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC25750.11113916565778201No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC25730.1110528439757177No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT25420.1097148579037211No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT25040.10807474594449948No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC24810.10708204660076007No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC24240.1046218786619276No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA23900.10315441006683455No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG23870.10302492754373813No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG23620.1019459065179344No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG23570.10173010231277367No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC23460.10125533306142004No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA23360.10082372465109857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG28650.052.388812
ACCTGGG29750.050.61143
CTGGGGG24800.047.3065575
GTACCTG32200.047.087051
CCTGGGG28600.045.8373074
GTACCCG3350.039.7264631
TATCACG1700.036.322062
TGGGGGG65200.033.2923626
CTGTGCG34850.031.893239
TAGTACT36350.030.707154
GTACACG2550.029.8226661
ACTGTGC39400.029.5362458
GGGGGGA67850.029.1918937
TACCCCG1802.1100277E-1029.0266045
TAGGCAT30900.028.2836445
TCTAACG11350.028.0385152
GTACTGT40300.027.8152546
CTAGTAC43250.027.5653613
TACTGTG43800.026.893897
CTAACGC11600.026.6153033
Produced by FastQC (version 0.11.5)