##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931100_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2316915 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64645617124495 33.0 33.0 33.0 33.0 33.0 2 32.59921404108481 33.0 33.0 33.0 33.0 33.0 3 32.658721619049466 33.0 33.0 33.0 33.0 33.0 4 36.60718584842344 37.0 37.0 37.0 37.0 37.0 5 36.55556116646489 37.0 37.0 37.0 37.0 37.0 6 36.55565223583947 37.0 37.0 37.0 37.0 37.0 7 36.4951200195087 37.0 37.0 37.0 37.0 37.0 8 36.504273139066385 37.0 37.0 37.0 37.0 37.0 9 36.494927522157695 37.0 37.0 37.0 37.0 37.0 10-11 36.4734923378717 37.0 37.0 37.0 37.0 37.0 12-13 36.380212480820404 37.0 37.0 37.0 37.0 37.0 14-15 38.32617899232385 40.0 37.0 40.0 37.0 40.0 16-17 38.31327541148467 40.0 37.0 40.0 37.0 40.0 18-19 38.25366770036881 40.0 37.0 40.0 37.0 40.0 20-21 38.1640295392796 40.0 37.0 40.0 35.0 40.0 22-23 38.01008496211557 40.0 37.0 40.0 33.0 40.0 24-25 38.17823787234318 40.0 37.0 40.0 37.0 40.0 26-27 38.12218057201063 40.0 37.0 40.0 35.0 40.0 28-29 38.0391697580619 40.0 37.0 40.0 33.0 40.0 30-31 37.907224045767755 40.0 37.0 40.0 33.0 40.0 32-33 37.78704419454317 40.0 37.0 40.0 33.0 40.0 34-35 37.70606819844491 40.0 37.0 40.0 33.0 40.0 36-37 37.64965503697805 40.0 37.0 40.0 33.0 40.0 38-39 37.48540300356292 40.0 37.0 40.0 33.0 40.0 40-41 37.2910344142966 40.0 37.0 40.0 33.0 40.0 42-43 37.104783516011594 40.0 37.0 40.0 33.0 40.0 44-45 36.84118234807923 40.0 37.0 40.0 33.0 40.0 46-47 36.565851789987974 37.0 37.0 40.0 33.0 40.0 48-49 36.27526085333299 37.0 37.0 40.0 33.0 40.0 50-51 36.0027096376 37.0 37.0 40.0 33.0 40.0 52-53 35.73951849765744 37.0 37.0 40.0 30.0 40.0 54-55 35.46682636177849 37.0 37.0 40.0 27.0 40.0 56-57 35.11504457435858 37.0 33.0 40.0 27.0 40.0 58-59 34.7923016165893 37.0 33.0 40.0 27.0 40.0 60-61 34.52869526935602 37.0 33.0 40.0 27.0 40.0 62-63 34.18261071295235 37.0 33.0 37.0 27.0 40.0 64-65 33.80774413390219 37.0 33.0 37.0 27.0 40.0 66-67 33.4335830187987 37.0 33.0 37.0 27.0 40.0 68-69 33.08636872738102 37.0 33.0 37.0 24.5 40.0 70-71 32.81628113245415 37.0 33.0 37.0 22.0 40.0 72-73 32.49248397114266 37.0 33.0 37.0 22.0 38.5 74-75 32.09228521546971 37.0 33.0 37.0 22.0 37.0 76-77 30.41359026982 33.0 30.0 37.0 22.0 37.0 78-79 31.25944391572414 33.0 33.0 37.0 22.0 37.0 80-81 31.34069829924706 33.0 33.0 37.0 22.0 37.0 82-83 31.11230709801611 33.0 33.0 37.0 18.5 37.0 84-85 30.838921151617562 33.0 33.0 37.0 15.0 37.0 86-87 30.51172032638228 33.0 33.0 37.0 15.0 37.0 88-89 30.18596841058045 33.0 30.0 37.0 15.0 37.0 90-91 29.770205208218687 33.0 27.0 37.0 6.0 37.0 92-93 29.346677586359448 33.0 27.0 37.0 4.0 37.0 94-95 28.841353265009722 33.0 27.0 37.0 2.0 37.0 96-97 28.31951668490212 33.0 27.0 37.0 2.0 37.0 98-99 27.616814600449302 33.0 27.0 37.0 2.0 37.0 100-101 25.851805094274066 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08803870012789616 1101 2 0.16597993422558233 1101 3 0.09872296712619999 1101 4 0.06390649556745132 1101 5 0.03550431127492004 1101 6 0.0318965394401971 1101 7 0.010324957382863431 1101 8 0.16967008402042438 1101 9 0.13390913675614513 1101 10-11 0.12565826839927752 1101 12-13 0.1801326050332861 1101 14-15 0.18113611411749986 1101 16-17 0.16647270381609047 1101 18-19 0.15871031328604346 1101 20-21 0.15264801128629557 1101 22-23 0.1227349473711783 1101 24-25 0.1002166421765125 1101 26-27 0.07769784027480853 1101 28-29 0.022326766652049912 1101 30-31 0.06075892741894506 1101 32-33 0.03149803767978199 1101 34-35 -0.052061077372883346 1101 36-37 -0.22476650987172775 1101 38-39 -0.21349084603383517 1101 40-41 -0.2518052768598267 1101 42-43 -0.23518440483327652 1101 44-45 -0.38695614771128106 1101 46-47 -0.33613779158208956 1101 48-49 -0.48210092844797003 1101 50-51 -0.4082741304801516 1101 52-53 -0.6344343918978907 1101 54-55 -0.7503798571558988 1101 56-57 -0.8238793217268849 1101 58-59 -0.8355169617830569 1101 60-61 -0.7631178076028462 1101 62-63 -0.7216807090665611 1101 64-65 -0.6952625274132629 1101 66-67 -0.9312523847721366 1101 68-69 -1.0073420789195424 1101 70-71 -1.0106738456661795 1101 72-73 -1.1844591619362532 1101 74-75 -1.234262484034538 1101 76-77 -1.3379711702285029 1101 78-79 -1.2513066779016704 1101 80-81 -1.5307528982448346 1101 82-83 -1.7187807611657853 1101 84-85 -1.6320285497713911 1101 86-87 -1.7271803370664003 1101 88-89 -1.9819547263319777 1101 90-91 -1.97507563252584 1101 92-93 -2.3222409295953845 1101 94-95 -2.4671974476278393 1101 96-97 -2.6023157798719083 1101 98-99 -2.5262761238797076 1101 100-101 -4.1849914336654415 1102 1 0.12912006773987628 1102 2 0.18433402911028907 1102 3 0.15855599124493835 1102 4 0.15121544512129503 1102 5 0.1635854138458157 1102 6 0.13382692198282342 1102 7 0.10925154100332435 1102 8 0.11213408260687174 1102 9 0.09686964563502443 1102 10-11 0.1491254474787027 1102 12-13 0.14128199985614742 1102 14-15 0.1911082848039598 1102 16-17 0.16918937490010677 1102 18-19 0.09460350173025489 1102 20-21 0.0025353688267131247 1102 22-23 0.08080323962314395 1102 24-25 0.16647691251841223 1102 26-27 0.18569103755372396 1102 28-29 0.16065612436916155 1102 30-31 0.16321939212431147 1102 32-33 0.07863117664965102 1102 34-35 0.12855337620075602 1102 36-37 0.007906429433866435 1102 38-39 0.1016540430906474 1102 40-41 -0.03257212905525364 1102 42-43 0.03011067423730651 1102 44-45 -0.10436713808146436 1102 46-47 -0.08508867063500958 1102 48-49 -0.24829052116817962 1102 50-51 -0.11899283354579637 1102 52-53 -0.0772517785928315 1102 54-55 -0.2470999737739774 1102 56-57 -0.2287295735159134 1102 58-59 -0.22633107963799404 1102 60-61 -0.4378571838728291 1102 62-63 -0.45271922837039114 1102 64-65 -0.5112688000521857 1102 66-67 -0.37583671200953006 1102 68-69 -0.534365225840645 1102 70-71 -0.5573762928788284 1102 72-73 -0.8214301841113567 1102 74-75 -0.7431965771523004 1102 76-77 -0.8061993705995611 1102 78-79 -0.6687574244340482 1102 80-81 -0.9608814431692991 1102 82-83 -0.9924798511914084 1102 84-85 -0.890962642889157 1102 86-87 -0.9220959656554975 1102 88-89 -1.1582843490901702 1102 90-91 -1.2941519643572725 1102 92-93 -1.4031226329260704 1102 94-95 -1.4672747512765731 1102 96-97 -1.4875419482613417 1102 98-99 -1.5820451847508075 1102 100-101 -2.613518688723307 1103 1 0.07273323609172166 1103 2 0.18284451978105665 1103 3 0.061059121099987124 1103 4 0.12566166608319662 1103 5 0.03970893543971954 1103 6 0.0921018062159007 1103 7 0.13420532641007554 1103 8 0.05597141059053712 1103 9 0.13433447142737265 1103 10-11 0.13136802423347405 1103 12-13 0.13421028720951966 1103 14-15 0.15950659510122733 1103 16-17 0.08601400078396892 1103 18-19 0.08720602438503988 1103 20-21 0.08834346639439161 1103 22-23 0.1703671632463042 1103 24-25 0.2375233650979638 1103 26-27 0.16378728960468436 1103 28-29 0.1447026624226666 1103 30-31 0.1019169701947078 1103 32-33 0.146653778644378 1103 34-35 0.14851845757699067 1103 36-37 0.10941156708882716 1103 38-39 0.08251311497759417 1103 40-41 -0.060517921809406516 1103 42-43 0.07649768186124817 1103 44-45 0.19962579439579287 1103 46-47 0.10614442521480782 1103 48-49 0.22660737097709927 1103 50-51 0.14067096052566086 1103 52-53 0.036221597946749284 1103 54-55 -0.01592959234226754 1103 56-57 0.020447530190807583 1103 58-59 0.14857841852934683 1103 60-61 0.10337582817899005 1103 62-63 -0.05042622198330804 1103 64-65 -0.10282334500394796 1103 66-67 -0.03607565360735521 1103 68-69 -0.07703704943580902 1103 70-71 -0.3678660837693215 1103 72-73 -0.36282450730010396 1103 74-75 -0.37219206546834727 1103 76-77 -0.5423431629280238 1103 78-79 -0.38860879193349973 1103 80-81 -0.47586431485948566 1103 82-83 -0.3723483051016707 1103 84-85 -0.42697839188400266 1103 86-87 -0.3352778927747089 1103 88-89 -0.6258949582513047 1103 90-91 -0.6369668311268448 1103 92-93 -0.7331208061298184 1103 94-95 -0.7072053749179936 1103 96-97 -0.8641579925603153 1103 98-99 -0.7232607249210545 1103 100-101 -1.542715669314017 1104 1 0.12400839385728801 1104 2 0.21729407771012887 1104 3 0.15268330512795103 1104 4 0.15464666133895122 1104 5 0.057546055186669776 1104 6 0.12035231187606144 1104 7 0.12120166713501135 1104 8 0.15939493046427344 1104 9 0.09657511975814259 1104 10-11 0.09105332737608762 1104 12-13 0.09195188738292615 1104 14-15 0.11567532971536565 1104 16-17 0.19068525481717558 1104 18-19 0.17189297414156357 1104 20-21 0.1978571624298695 1104 22-23 0.11389948338317168 1104 24-25 0.15486783792405134 1104 26-27 0.19447588158413964 1104 28-29 0.25025839760883173 1104 30-31 0.14762427768757647 1104 32-33 0.17014776560958467 1104 34-35 0.18337218437320502 1104 36-37 0.1963301277606675 1104 38-39 0.18988032504680774 1104 40-41 0.15488652049543816 1104 42-43 0.02621737827753634 1104 44-45 0.18521502869437967 1104 46-47 0.15009454618604678 1104 48-49 0.01287379270745248 1104 50-51 0.06508409735109666 1104 52-53 0.11003101625893663 1104 54-55 -0.07324393113596983 1104 56-57 0.0631781589258722 1104 58-59 -0.013824715665492704 1104 60-61 -0.015306114274544314 1104 62-63 -0.10066970129245334 1104 64-65 -0.04380181687439233 1104 66-67 -0.02563622125603615 1104 68-69 -0.06089294816566593 1104 70-71 0.052773413169177275 1104 72-73 -0.04407278833497941 1104 74-75 -0.10733232304267304 1104 76-77 -0.20042348655052322 1104 78-79 0.09756703609374284 1104 80-81 -0.12041496091094217 1104 82-83 -0.05596421733974566 1104 84-85 -0.043053694631428385 1104 86-87 -0.09646758229717634 1104 88-89 -0.043053712988065485 1104 90-91 -0.11433387354082569 1104 92-93 -0.12731318891386323 1104 94-95 -0.09311918176559786 1104 96-97 0.0034100405364299036 1104 98-99 -0.12348909530020435 1104 100-101 -0.9776135453217947 1105 1 0.06206228639637601 1105 2 0.17545734166217386 1105 3 0.012095523355903026 1105 4 0.12526231202530624 1105 5 0.1487779347526228 1105 6 0.11158993546012397 1105 7 0.039807792988149515 1105 8 0.059942280415064886 1105 9 0.061793289130093854 1105 10-11 0.04527321862646971 1105 12-13 0.08101161815719848 1105 14-15 0.15467598051451148 1105 16-17 0.15248203676420502 1105 18-19 0.11691779190169171 1105 20-21 0.022597406745887838 1105 22-23 0.08371779610913421 1105 24-25 0.12831398149834428 1105 26-27 0.20057582029565424 1105 28-29 0.10009184951917405 1105 30-31 0.07087983549903498 1105 32-33 0.1607541159154522 1105 34-35 0.13252511231534214 1105 36-37 0.21512965451423582 1105 38-39 0.23132569061943542 1105 40-41 0.19184245250218623 1105 42-43 0.20555443896678582 1105 44-45 0.11243756086609125 1105 46-47 0.08422790939585667 1105 48-49 0.04268041265989808 1105 50-51 0.013277888010641448 1105 52-53 0.09372160666627138 1105 54-55 0.008040370652082629 1105 56-57 0.1374975069968869 1105 58-59 0.09074968387134419 1105 60-61 0.03717373758666298 1105 62-63 -0.0418113353196361 1105 64-65 0.16940096990329323 1105 66-67 0.2229141724556456 1105 68-69 0.11839444477616468 1105 70-71 0.017933478859511354 1105 72-73 -0.10115866026840337 1105 74-75 0.08173572992286893 1105 76-77 -0.00955602038478176 1105 78-79 0.10289665079145394 1105 80-81 0.10268374539589331 1105 82-83 0.1533245803273111 1105 84-85 0.31985555007189603 1105 86-87 0.18607621394525253 1105 88-89 0.05844792121352427 1105 90-91 0.03366634560389414 1105 92-93 0.18239052401565914 1105 94-95 0.009835707976744601 1105 96-97 0.010025424306032704 1105 98-99 -0.06567631123908058 1105 100-101 -0.7150761831379455 1106 1 0.057295931895978924 1106 2 0.17155754520918265 1106 3 0.11976436240772159 1106 4 0.1042937750993218 1106 5 0.004956681888813819 1106 6 0.13355224731605375 1106 7 0.12093508289203925 1106 8 0.12691141520335236 1106 9 0.10066574821532015 1106 10-11 0.06890646433418368 1106 12-13 0.05573482160379939 1106 14-15 0.2183565874132043 1106 16-17 0.06817118716079307 1106 18-19 0.046893170015358976 1106 20-21 0.16531135930027574 1106 22-23 0.15240122117553767 1106 24-25 0.17497291246578328 1106 26-27 0.28348015319998865 1106 28-29 0.15633739978068206 1106 30-31 0.23756247558593202 1106 32-33 0.15298618461028468 1106 34-35 0.18595791441835985 1106 36-37 0.16194957473840788 1106 38-39 0.23501640641121924 1106 40-41 0.33295735159580886 1106 42-43 0.3123319041219119 1106 44-45 0.29969738908337007 1106 46-47 0.24211547020895097 1106 48-49 0.21764241873892587 1106 50-51 0.2451974025099446 1106 52-53 0.2509305823592882 1106 54-55 0.40442792461942645 1106 56-57 0.35819426758725825 1106 58-59 0.38055601756065727 1106 60-61 0.34837561195342914 1106 62-63 0.40482289389438364 1106 64-65 0.4121142378825198 1106 66-67 0.4579559201303738 1106 68-69 0.3720640528340695 1106 70-71 0.47964804296769614 1106 72-73 0.6319836765973363 1106 74-75 0.5648814896643728 1106 76-77 0.4767225263990831 1106 78-79 0.5909248575696608 1106 80-81 0.4700444571714968 1106 82-83 0.7199049334768155 1106 84-85 0.8842506822861758 1106 86-87 0.6897706378524404 1106 88-89 0.8043746990551988 1106 90-91 0.7513190286821079 1106 92-93 0.878971128176051 1106 94-95 0.8930392002227912 1106 96-97 1.0560445627453845 1106 98-99 1.077727065541957 1106 100-101 0.7564203894224555 1107 1 0.07800495547557773 1107 2 0.1921646129819905 1107 3 0.08905440105278473 1107 4 0.11492989209878601 1107 5 0.11429801466384504 1107 6 0.09486683386617756 1107 7 0.09594827717291565 1107 8 0.10912694214585628 1107 9 0.1620464152134744 1107 10-11 0.0980260241468045 1107 12-13 0.059222037327025134 1107 14-15 0.18305016387024153 1107 16-17 0.144878756098187 1107 18-19 0.15920757773390903 1107 20-21 0.17908520714513543 1107 22-23 0.08637965670457248 1107 24-25 0.0973594634589503 1107 26-27 0.14623128071126246 1107 28-29 0.10300224541850156 1107 30-31 0.08424929351793509 1107 32-33 0.16089975443746596 1107 34-35 0.12402821267453845 1107 36-37 0.18862819258841768 1107 38-39 0.21023105882369464 1107 40-41 0.28581887489189484 1107 42-43 0.3331060812785793 1107 44-45 0.47658689734015525 1107 46-47 0.49871751761240546 1107 48-49 0.42852355680562226 1107 50-51 0.4759334798175203 1107 52-53 0.4365689347534456 1107 54-55 0.4876137764247517 1107 56-57 0.4870980300291379 1107 58-59 0.5928503612487717 1107 60-61 0.648841282833537 1107 62-63 0.6816844324565707 1107 64-65 0.7508326915564183 1107 66-67 0.7911117849336975 1107 68-69 0.7840273945419582 1107 70-71 0.819473289624419 1107 72-73 0.7999337869495022 1107 74-75 0.8289982797684416 1107 76-77 0.7224144846484499 1107 78-79 0.9689614504355859 1107 80-81 0.9416740271906008 1107 82-83 0.9477257289720029 1107 84-85 1.072288267449263 1107 86-87 1.1326601199387518 1107 88-89 1.1266859008906174 1107 90-91 1.013430506179862 1107 92-93 1.2347072054836303 1107 94-95 1.1170312007810956 1107 96-97 1.2160936432893799 1107 98-99 1.5114266219524168 1107 100-101 2.134459435770367 1108 1 0.05007470023076621 1108 2 0.11264025719312087 1108 3 0.045926868734959214 1108 4 0.09797932965010148 1108 5 0.10933982554707455 1108 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0.7295194610053777 2316 86-87 0.7777635726660428 2316 88-89 0.6189165574333266 2316 90-91 0.7860473161397543 2316 92-93 0.9352439759804092 2316 94-95 1.1165518794411184 2316 96-97 1.2476910265505587 2316 98-99 1.7474760285742832 2316 100-101 2.1150618867377453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 18.0 5 32.0 6 65.0 7 176.0 8 488.0 9 994.0 10 1673.0 11 2584.0 12 3755.0 13 5381.0 14 6477.0 15 8389.0 16 10596.0 17 13836.0 18 14074.0 19 11461.0 20 10958.0 21 11315.0 22 12400.0 23 14031.0 24 16329.0 25 19378.0 26 23318.0 27 28354.0 28 34944.0 29 43088.0 30 54430.0 31 69407.0 32 91072.0 33 123584.0 34 177257.0 35 271396.0 36 427619.0 37 576944.0 38 231074.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.767758701273344 22.17868981765058 13.051499650540862 25.00205183053521 2 14.373121154638818 20.90124152159229 40.39461956955693 24.33101775421196 3 17.241676971317464 28.102023596031795 29.453216885384233 25.203082547266515 4 12.035271039291471 17.45515049106247 38.88735667903225 31.622221790613814 5 12.693214899985541 38.21801835630569 35.064428345450736 14.02433839825803 6 29.312814669506647 38.58868365908978 18.540343517133774 13.558158154269794 7 26.463897035497634 31.669051303133696 24.213663427445546 17.653388233923124 8 26.559368815860747 34.84465334291504 19.92740346538393 18.66857437584029 9 26.662825351814806 14.408383561762083 21.82315708603898 37.10563400038413 10-11 25.906151067259696 25.786142348769808 27.697239648411788 20.610466935558705 12-13 25.739917088024377 23.820360263540096 27.949342120880566 22.490380527554958 14-15 23.009751354066992 24.91237247556982 25.609270800018475 26.46860537034471 16-17 21.96135809902392 27.06996156527106 26.927768174490645 24.040912161214372 18-19 21.54092834652976 27.707101900587634 28.771146114553186 21.980823638329415 20-21 22.873519313397342 26.821743568495176 27.85682685812816 22.447910259979327 22-23 23.407289434441918 26.66938579965169 27.321092918816614 22.602231847089772 24-25 22.78605386904569 26.64087806414996 28.32041745165446 22.25265061514989 26-27 22.81730231795297 26.778172699473224 27.2912687776634 23.11325620491041 28-29 22.06777115258868 27.275666133630278 27.875364439351465 22.781198274429578 30-31 22.99357119272826 26.933875433496695 27.663833157452906 22.408720216322138 32-33 23.334374229174188 27.250315020256465 26.779235919150228 22.636074831419116 34-35 23.02531599130741 26.88911764134636 27.889564356051043 22.196002011295192 36-37 23.009324899704993 26.349822932649662 27.598638707073846 23.042213460571492 38-39 23.054675313801027 26.449535891276067 28.200140359115615 22.295648435807287 40-41 22.53036796350881 25.74251143694863 28.609442286086225 23.117678313456345 42-43 22.881691728771596 26.087307458893346 27.71679030101287 23.31421051132219 44-45 22.683136843604533 27.15813916349974 27.62902393916048 22.529700053735247 46-47 23.010965011664215 26.625922832732318 27.155700575981424 23.20741157962204 48-49 23.699524129428337 26.283006493774145 27.337794300731815 22.679675076065703 50-51 23.005742549899328 26.43750418120647 27.01788801056577 23.53886525832842 52-53 22.549057690938167 26.900447362117298 26.901655865666196 23.64883908127834 54-55 22.600246448402295 26.921747237166667 26.806637274133926 23.67136904029712 56-57 23.824374221756084 26.327724582041206 26.551945151203217 23.295956044999492 58-59 22.616928113461217 26.71623689259209 27.50385750016725 23.162977493779444 60-61 22.92969314800068 27.577468314547577 26.482952546813326 23.009885990638413 62-63 23.32262944475736 26.643036106201567 26.027540932662614 24.006793516378462 64-65 22.281460476538843 26.959469812228757 26.705921451585407 24.053148259646985 66-67 23.032195493356053 26.319919407677506 26.698472837642207 23.949412261324234 68-69 22.50790714940115 26.454672690955906 26.836266163659378 24.201153995983567 70-71 22.466734372632864 26.26310975833459 26.906409953726584 24.363745915305966 72-73 23.223381090803937 26.168417917791547 27.19348789230507 23.414713099099448 74-75 23.158402444630035 26.793645860983244 26.71539525619196 23.332556438194754 76-77 22.969754518478556 26.519233881845157 26.531103318046938 23.97990828162935 78-79 22.6008795764152 26.80586602969531 26.56350556393158 24.02974882995791 80-81 22.116162164413616 27.166313848520485 27.279162421446795 23.438361565619104 82-83 23.123110550187068 25.854816877675013 28.295088250578814 22.726984321559108 84-85 23.384718176103167 26.528192101088386 26.712903026009084 23.37418669679936 86-87 21.995427955336137 27.2068165029534 27.275485652960104 23.522269888750365 88-89 22.871663397232957 26.03127434541189 27.096246517459637 24.000815739895508 90-91 22.52415874647079 26.016518952253094 27.3594040695071 24.099918231769017 92-93 22.98188198200823 26.200287669799096 27.003000786613967 23.81482956157871 94-95 21.520316640912977 26.351529251301354 27.80203036921297 24.3261237385727 96-97 21.939919420365296 26.533010393257882 26.982718044438574 24.54435214193825 98-99 21.291868713353747 27.13707667307605 26.845762576529562 24.72529203704063 100-101 20.04775752213344 27.466250883449415 26.766807119388407 25.71918447502873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 74.0 1 51.5 2 31.0 3 36.5 4 39.0 5 39.0 6 65.0 7 133.0 8 198.0 9 257.0 10 279.0 11 299.0 12 385.0 13 500.0 14 622.0 15 769.5 16 874.0 17 1027.0 18 1217.5 19 1411.5 20 1618.0 21 1778.5 22 1944.0 23 2148.5 24 2508.0 25 2989.0 26 3771.0 27 4798.5 28 6969.0 29 12485.0 30 19792.0 31 25747.5 32 33680.5 33 41457.5 34 48271.0 35 56209.5 36 60993.0 37 69625.5 38 73123.0 39 68588.0 40 64079.0 41 59221.0 42 58790.0 43 62173.0 44 68719.5 45 81784.0 46 94938.5 47 104234.5 48 111431.5 49 109117.0 50 104478.5 51 105805.0 52 104209.0 53 99488.0 54 95848.5 55 85484.5 56 73415.5 57 60235.0 58 48598.0 59 46435.5 60 39057.5 61 27112.0 62 19483.5 63 15425.0 64 11358.5 65 6165.0 66 3596.5 67 2393.5 68 1754.5 69 1330.0 70 989.5 71 737.5 72 552.5 73 416.5 74 324.5 75 229.0 76 182.0 77 139.0 78 93.5 79 74.5 80 52.5 81 36.0 82 28.5 83 24.5 84 26.5 85 23.0 86 14.5 87 10.0 88 9.5 89 8.0 90 6.0 91 3.5 92 1.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0823940455303712 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.589650461928901E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 2.1580420516074175E-5 34-35 0.0 36-37 0.0 38-39 4.316084103214835E-5 40-41 0.0035391889646361648 42-43 0.005913035221404324 44-45 0.0 46-47 0.0 48-49 1.5106294361251924E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002978098031218236 68-69 0.007876853488367074 70-71 0.001467468595093044 72-73 0.0 74-75 0.0 76-77 0.0017264336412859342 78-79 0.0050929792417935054 80-81 0.0040355386365058706 82-83 0.00444556662631128 84-85 0.00222278331315564 86-87 3.66867148773261E-4 88-89 0.0 90-91 2.1580420516074175E-5 92-93 9.711189232233379E-4 94-95 0.004100279898054093 96-97 0.0033018043389593486 98-99 0.0 100-101 1.0790210258037089E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2316915.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.45641854067433 #Duplication Level Percentage of deduplicated Percentage of total 1 81.19964652740467 25.54250066520854 2 10.9278497591935 6.875020315495957 3 2.9674226546545097 2.8003346703567344 4 1.2285692083503401 1.5458554889621288 5 0.6706080671780841 1.054746401895323 6 0.4235496869780665 0.7994013735812195 7 0.2901588618086003 0.638915102023591 8 0.20792431867720254 0.5232443514475705 9 0.16184828605980234 0.4582050683749136 >10 1.289942427412628 8.398888563223792 >50 0.24775737792394792 5.512394301016711 >100 0.3084691048470255 21.208298727959736 >500 0.054151192117054915 11.577705664852518 >1k 0.021135541821197455 11.068944511385094 >5k 9.669855735188378E-4 1.995544794216309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9960 0.42988197668019756 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 7278 0.3141246010319757 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 6764 0.29193992874145147 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5676 0.24498093369847404 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 5642 0.243513465103381 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 5386 0.232464289799151 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5217 0.22517010766471796 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 3800 0.16401119592216373 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3492 0.15071765688426203 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 3294 0.14217181035989665 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3262 0.14079066344686791 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 3259 0.14066118092377147 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 3206 0.13837365634906762 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 3194 0.13785572625668185 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3125 0.1348776282254636 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 2951 0.12736764188586977 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 2925 0.12624546001903394 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 2876 0.12413057880845865 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 2824 0.12188621507478695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2768 0.11946920797698662 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 2671 0.11528260639686826 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2661 0.11485099798654677 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 2583 0.1114844523860392 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 2583 0.1114844523860392 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 2575 0.11113916565778201 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2573 0.1110528439757177 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 2542 0.1097148579037211 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2504 0.10807474594449948 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 2481 0.10708204660076007 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2424 0.1046218786619276 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 2390 0.10315441006683455 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 2387 0.10302492754373813 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 2362 0.1019459065179344 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 2357 0.10173010231277367 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 2346 0.10125533306142004 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2336 0.10082372465109857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.316084103214835E-5 0.0 5 0.0 0.0 0.0 4.316084103214835E-5 0.0 6 0.0 0.0 0.0 4.316084103214835E-5 0.0 7 0.0 0.0 0.0 8.63216820642967E-5 0.0 8 0.0 0.0 0.0 8.63216820642967E-5 0.0 9 0.0 0.0 0.0 8.63216820642967E-5 0.0 10-11 0.0 0.0 0.0 8.63216820642967E-5 0.0 12-13 0.0 0.0 0.0 8.63216820642967E-5 0.0 14-15 0.0 0.0 0.0 8.63216820642967E-5 0.0 16-17 0.0 0.0 0.0 8.63216820642967E-5 4.316084103214835E-5 18-19 0.0 0.0 0.0 1.2948252309644506E-4 8.63216820642967E-5 20-21 0.0 0.0 0.0 1.2948252309644506E-4 8.63216820642967E-5 22-23 0.0 0.0 0.0 2.589650461928901E-4 8.63216820642967E-5 24-25 0.0 0.0 0.0 3.452867282571868E-4 8.63216820642967E-5 26-27 0.0 0.0 0.0 6.689930359982995E-4 8.63216820642967E-5 28-29 0.0 0.0 0.0 0.0021580420516074178 8.63216820642967E-5 30-31 0.0 0.0 0.0 0.0070783779292723295 8.63216820642967E-5 32-33 0.0 0.0 0.0 0.02252995901878144 8.63216820642967E-5 34-35 0.0 0.0 0.0 0.04749850555587926 8.63216820642967E-5 36-37 0.0 0.0 0.0 0.085825332392427 8.63216820642967E-5 38-39 0.0 0.0 0.0 0.13902106896454985 8.63216820642967E-5 40-41 0.0 0.0 0.0 0.19415904338311935 8.63216820642967E-5 42-43 0.0 0.0 0.0 0.25199457036619816 8.63216820642967E-5 44-45 0.0 0.0 0.0 0.32135404190486055 8.63216820642967E-5 46-47 0.0 0.0 0.0 0.4049134301430998 1.0790210258037087E-4 48-49 0.0 0.0 0.0 0.495184329161838 1.2948252309644506E-4 50-51 0.0 0.0 0.0 0.5889944171452125 1.726433641285934E-4 52-53 0.0 0.0 0.0 0.6827397638670387 1.9422378464466759E-4 54-55 0.0 0.0 0.0 0.7856567893081965 2.1580420516074177E-4 56-57 0.0 0.0 0.0 0.9096147247525266 2.1580420516074177E-4 58-59 0.0 0.0 0.0 1.0526497519330662 2.1580420516074177E-4 60-61 0.0 0.0 0.0 1.2032379262942317 2.1580420516074177E-4 62-63 0.0 0.0 0.0 1.355466212614619 2.1580420516074177E-4 64-65 0.0 0.0 0.0 1.5158303174695662 2.1580420516074177E-4 66-67 0.0 0.0 0.0 1.7004076541435484 2.3738462567681596E-4 68-69 0.0 0.0 0.0 1.9078818169850857 2.589650461928901E-4 70-71 0.0 0.0 0.0 2.1326418966599983 2.589650461928901E-4 72-73 0.0 0.0 0.0 2.367048424305596 3.452867282571868E-4 74-75 0.0 0.0 0.0 2.595995105560627 3.6686714877326096E-4 76-77 0.0 0.0 0.0 2.8535574244199724 3.8844756928933517E-4 78-79 0.0 0.0 0.0 3.1355703597240296 3.8844756928933517E-4 80-81 0.0 0.0 0.0 3.448119590058332 3.8844756928933517E-4 82-83 0.0 0.0 0.0 3.770509492147964 4.3160841032148354E-4 84-85 0.0 0.0 0.0 4.0941942194685605 4.531888308375577E-4 86-87 0.0 0.0 0.0 4.440257842864327 4.7476925135363186E-4 88-89 0.0 0.0 0.0 4.8171383067570455 4.7476925135363186E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2865 0.0 52.38881 2 ACCTGGG 2975 0.0 50.6114 3 CTGGGGG 2480 0.0 47.306557 5 GTACCTG 3220 0.0 47.08705 1 CCTGGGG 2860 0.0 45.837307 4 GTACCCG 335 0.0 39.726463 1 TATCACG 170 0.0 36.32206 2 TGGGGGG 6520 0.0 33.292362 6 CTGTGCG 3485 0.0 31.89323 9 TAGTACT 3635 0.0 30.70715 4 GTACACG 255 0.0 29.822666 1 ACTGTGC 3940 0.0 29.536245 8 GGGGGGA 6785 0.0 29.191893 7 TACCCCG 180 2.1100277E-10 29.026604 5 TAGGCAT 3090 0.0 28.283644 5 TCTAACG 1135 0.0 28.038515 2 GTACTGT 4030 0.0 27.815254 6 CTAGTAC 4325 0.0 27.565361 3 TACTGTG 4380 0.0 26.89389 7 CTAACGC 1160 0.0 26.615303 3 >>END_MODULE