##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931098_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5292410 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483772043360208 33.0 33.0 33.0 27.0 33.0 2 31.440417314607146 33.0 33.0 33.0 27.0 33.0 3 31.4370056363736 33.0 33.0 33.0 27.0 33.0 4 35.13594354934708 37.0 37.0 37.0 33.0 37.0 5 35.12953342617069 37.0 37.0 37.0 33.0 37.0 6 35.2054687750949 37.0 37.0 37.0 33.0 37.0 7 35.17962818451329 37.0 37.0 37.0 33.0 37.0 8 35.181066281712866 37.0 37.0 37.0 33.0 37.0 9 35.18898611407657 37.0 37.0 37.0 33.0 37.0 10-11 35.1044273780754 37.0 37.0 37.0 33.0 37.0 12-13 35.04318174517847 37.0 37.0 37.0 33.0 37.0 14-15 36.82962298839281 40.0 37.0 40.0 33.0 40.0 16-17 36.80121466401885 40.0 37.0 40.0 33.0 40.0 18-19 36.75844671898058 40.0 37.0 40.0 33.0 40.0 20-21 36.6861389234772 40.0 37.0 40.0 33.0 40.0 22-23 36.62941221485108 40.0 37.0 40.0 33.0 40.0 24-25 36.5344567030899 40.0 37.0 40.0 33.0 40.0 26-27 36.38071814164057 40.0 37.0 40.0 33.0 40.0 28-29 36.29898543385717 40.0 37.0 40.0 33.0 40.0 30-31 36.17880785880156 40.0 37.0 40.0 33.0 40.0 32-33 36.041212887890396 40.0 37.0 40.0 33.0 40.0 34-35 35.95234260006311 40.0 37.0 40.0 33.0 40.0 36-37 35.78799875671008 40.0 37.0 40.0 30.0 40.0 38-39 35.632815295867104 40.0 37.0 40.0 27.0 40.0 40-41 35.43146080896983 37.0 37.0 40.0 27.0 40.0 42-43 35.29199532916006 37.0 37.0 40.0 27.0 40.0 44-45 35.06924992583719 37.0 37.0 40.0 27.0 40.0 46-47 34.89462957329459 37.0 37.0 40.0 27.0 40.0 48-49 34.917697419512095 37.0 37.0 40.0 27.0 40.0 50-51 34.13135046226577 37.0 35.0 38.5 22.0 40.0 52-53 34.2412690059916 37.0 35.0 40.0 24.5 40.0 54-55 34.79686258245299 37.0 37.0 40.0 27.0 40.0 56-57 34.79415568710663 37.0 37.0 40.0 27.0 40.0 58-59 34.67606326796299 37.0 37.0 40.0 27.0 40.0 60-61 34.71252132771271 37.0 37.0 40.0 27.0 40.0 62-63 34.59215688126959 37.0 37.0 40.0 24.5 40.0 64-65 34.41227607082595 37.0 35.0 40.0 22.0 40.0 66-67 34.2287455998307 37.0 33.0 40.0 22.0 40.0 68-69 34.05164235197198 37.0 33.0 40.0 22.0 40.0 70-71 33.86901808438878 37.0 33.0 40.0 22.0 40.0 72-73 33.295938334331616 37.0 33.0 40.0 15.0 40.0 74-75 33.118098087638714 37.0 33.0 38.5 15.0 40.0 76-77 32.7403488202917 37.0 33.0 37.0 15.0 40.0 78-79 32.47548668753933 37.0 33.0 37.0 15.0 40.0 80-81 32.1959535447934 37.0 33.0 37.0 15.0 37.0 82-83 31.91940477022755 37.0 33.0 37.0 15.0 37.0 84-85 31.543317789060183 37.0 33.0 37.0 6.0 37.0 86-87 31.364649091812616 37.0 33.0 37.0 6.0 37.0 88-89 31.145027029273997 37.0 33.0 37.0 2.0 37.0 90-91 30.882090011922735 37.0 33.0 37.0 2.0 37.0 92-93 30.607046411748144 37.0 33.0 37.0 2.0 37.0 94-95 30.381250791227437 37.0 33.0 37.0 2.0 37.0 96-97 30.120606585657576 33.0 33.0 37.0 2.0 37.0 98-99 29.83299054683972 33.0 33.0 37.0 2.0 37.0 100-101 29.194887773245078 33.0 30.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.012301764974342433 1101 2 -0.04030160636469304 1101 3 -0.03492332830616718 1101 4 0.032126764627541604 1101 5 -0.011743506961366279 1101 6 -0.0018953095068283687 1101 7 -0.011327402543102494 1101 8 -0.05934594112103042 1101 9 -0.013433826880941524 1101 10-11 -0.045634593064356466 1101 12-13 -0.0315659567648936 1101 14-15 0.023201999150593622 1101 16-17 0.0866955047581186 1101 18-19 0.12355133787290384 1101 20-21 0.129465030791593 1101 22-23 0.13537704857628796 1101 24-25 0.08570278439866996 1101 26-27 0.08829083437229457 1101 28-29 0.02256409339089771 1101 30-31 0.10125300806345905 1101 32-33 0.10231945549666221 1101 34-35 0.1485531054788396 1101 36-37 0.0531479062322191 1101 38-39 0.06317553724364444 1101 40-41 0.10630158797525269 1101 42-43 0.0524020503524909 1101 44-45 0.022298462464569013 1101 46-47 0.05077643323888026 1101 48-49 0.12454866550368848 1101 50-51 0.12409198604008509 1101 52-53 0.2333331724741612 1101 54-55 0.29895560912823527 1101 56-57 0.2817503012019813 1101 58-59 0.24647034364679143 1101 60-61 0.24399688665803154 1101 62-63 0.30504930615754233 1101 64-65 0.3803984224665342 1101 66-67 0.38712590790765233 1101 68-69 0.29160858981218496 1101 70-71 0.23972084624393375 1101 72-73 0.1456461126343811 1101 74-75 0.24641272337591857 1101 76-77 0.11590710123390835 1101 78-79 0.12388850127743467 1101 80-81 0.19177928276808842 1101 82-83 0.08421946464406815 1101 84-85 0.23885245857907833 1101 86-87 0.22781815482917978 1101 88-89 0.19102858313385696 1101 90-91 0.19433720496618534 1101 92-93 0.17546066486003653 1101 94-95 0.1402153912372306 1101 96-97 0.13805229488499648 1101 98-99 0.1804779358515063 1101 100-101 0.21423703226256308 1102 1 0.22281266203547645 1102 2 0.17672431218078088 1102 3 0.18663910831836006 1102 4 0.21756538736205044 1102 5 0.1415459555341556 1102 6 0.18303470934146304 1102 7 0.20108894183252346 1102 8 0.1804693632065053 1102 9 0.24229019555267683 1102 10-11 0.19095051654995387 1102 12-13 0.23800045762205713 1102 14-15 0.33298368853292004 1102 16-17 0.30792434295048565 1102 18-19 0.2975370468580323 1102 20-21 0.30871038971827147 1102 22-23 0.33667437479650886 1102 24-25 0.35811470609204576 1102 26-27 0.3273556450911812 1102 28-29 0.28139825716199596 1102 30-31 0.40004905923088785 1102 32-33 0.33688560198080353 1102 34-35 0.3026486079849633 1102 36-37 0.34124658900987015 1102 38-39 0.39732143290857636 1102 40-41 0.4599886164137317 1102 42-43 0.45861823676603564 1102 44-45 0.45736069546467206 1102 46-47 0.3838979435304708 1102 48-49 0.4324311251111652 1102 50-51 0.3981568132171702 1102 52-53 0.4865841456562734 1102 54-55 0.4980999065197409 1102 56-57 0.5610549141957932 1102 58-59 0.5275661109469567 1102 60-61 0.5413709956525992 1102 62-63 0.5139613126106113 1102 64-65 0.5321648620528023 1102 66-67 0.5317361422339175 1102 68-69 0.5912211502297495 1102 70-71 0.4777526996138661 1102 72-73 0.4261237376293323 1102 74-75 0.486179841771218 1102 76-77 0.4423257380529648 1102 78-79 0.4439513496310923 1102 80-81 0.5083508226021323 1102 82-83 0.5591358067338241 1102 84-85 0.6443721662596253 1102 86-87 0.46297638481268066 1102 88-89 0.55010580698778 1102 90-91 0.5250050729590399 1102 92-93 0.4721633252903885 1102 94-95 0.47566482151858835 1102 96-97 0.5656492286138857 1102 98-99 0.5570956848059083 1102 100-101 0.5017865142775086 1103 1 0.1091320107634175 1103 2 0.05895937322271294 1103 3 0.12479212142969587 1103 4 0.12482537492221013 1103 5 0.060848667932972944 1103 6 0.16719514355492038 1103 7 0.10124509730523812 1103 8 0.10454238507746538 1103 9 0.12435499736655231 1103 10-11 0.012660978915057797 1103 12-13 0.14634749148027026 1103 14-15 0.19093283061463495 1103 16-17 0.17644977599206868 1103 18-19 0.20566897910946835 1103 20-21 0.2444485752018224 1103 22-23 0.1818709631831794 1103 24-25 0.16399148997859214 1103 26-27 0.15588859497884755 1103 28-29 0.24345879003298165 1103 30-31 0.23389083537927746 1103 32-33 0.1162736253399217 1103 34-35 0.18800093414629515 1103 36-37 0.19698491802341067 1103 38-39 0.15771500245559622 1103 40-41 0.17454049042981268 1103 42-43 0.15609053612249824 1103 44-45 0.20195573848886283 1103 46-47 0.16823674168939817 1103 48-49 0.1441483247912103 1103 50-51 0.1271879410904475 1103 52-53 0.22310261861061775 1103 54-55 0.35311426854311634 1103 56-57 0.3912750934982654 1103 58-59 0.31394114415438423 1103 60-61 0.3829615219512945 1103 62-63 0.3548822758017067 1103 64-65 0.30620175218248136 1103 66-67 0.3645833286034872 1103 68-69 0.31976672559979136 1103 70-71 0.4280178390412246 1103 72-73 0.23049674405577747 1103 74-75 0.2847481988745244 1103 76-77 0.27753018964850895 1103 78-79 0.3706702867115865 1103 80-81 0.3605344423236332 1103 82-83 0.44092732142987856 1103 84-85 0.4559305031647334 1103 86-87 0.4103362236946779 1103 88-89 0.32025516154544675 1103 90-91 0.3336810354415043 1103 92-93 0.29566660185660965 1103 94-95 0.2876103456131922 1103 96-97 0.24493797563058806 1103 98-99 0.31106630877203045 1103 100-101 0.347208689695389 1104 1 0.020076004430546135 1104 2 0.00994600354884767 1104 3 0.01010652295078529 1104 4 0.03636683293562015 1104 5 -0.0023021861780065933 1104 6 0.0750866831090633 1104 7 0.073385973636384 1104 8 0.049471703918008814 1104 9 0.04618486657572163 1104 10-11 0.040228885685571925 1104 12-13 0.020044530712510777 1104 14-15 0.03669434088426016 1104 16-17 0.059247186729393775 1104 18-19 0.08910644075026397 1104 20-21 0.08800184722721127 1104 22-23 0.07663206512287246 1104 24-25 0.1058677284332532 1104 26-27 0.0912140189029742 1104 28-29 0.09751152722131451 1104 30-31 0.013306247203807686 1104 32-33 0.005192227398602256 1104 34-35 0.0557684215644727 1104 36-37 0.14371351890470407 1104 38-39 0.12643517620875144 1104 40-41 0.16387944685875055 1104 42-43 0.10449942719360195 1104 44-45 0.0518556600794966 1104 46-47 -0.15047604204353604 1104 48-49 -0.08284741905816873 1104 50-51 -0.11391397355106392 1104 52-53 -0.04978452450776416 1104 54-55 0.022638200935695352 1104 56-57 0.07305205517287305 1104 58-59 0.00991821755606992 1104 60-61 0.04836332611826322 1104 62-63 0.02711295966665972 1104 64-65 0.020698341500271056 1104 66-67 0.027711762144654983 1104 68-69 0.07238705141200796 1104 70-71 0.0732780804819484 1104 72-73 0.07139587153236704 1104 74-75 0.11345066163007544 1104 76-77 -0.010997492866202663 1104 78-79 0.1078028837149958 1104 80-81 0.19082826451724344 1104 82-83 0.08495192643956173 1104 84-85 0.25635298686284713 1104 86-87 0.20243053037300385 1104 88-89 0.1292644729709167 1104 90-91 0.24550619507846605 1104 92-93 0.24757761098265618 1104 94-95 0.23743774480204394 1104 96-97 0.2784980615051289 1104 98-99 0.287853503745815 1104 100-101 0.406784151822162 1105 1 0.14811306360180865 1105 2 0.09202593721882835 1105 3 0.06105542537684627 1105 4 0.06399400772261998 1105 5 0.021825802855786947 1105 6 0.1390114407842944 1105 7 0.09412193943357039 1105 8 0.13134729430061043 1105 9 0.09420316609152479 1105 10-11 0.13866270456366436 1105 12-13 0.1379595911028204 1105 14-15 0.17351484671996076 1105 16-17 0.1583756974506585 1105 18-19 0.16424785572186806 1105 20-21 0.24284486600547694 1105 22-23 0.23656245958414246 1105 24-25 0.1670484853448997 1105 26-27 0.24174695749655228 1105 28-29 0.20442387867880996 1105 30-31 0.169372180514749 1105 32-33 0.22396819641707566 1105 34-35 0.23941879649014908 1105 36-37 0.1780381061444416 1105 38-39 0.17749743736723644 1105 40-41 0.1733649680915832 1105 42-43 0.20316054737450173 1105 44-45 0.16028459656553196 1105 46-47 0.06749539826061124 1105 48-49 0.1494648519878936 1105 50-51 0.16745032920967873 1105 52-53 0.1832547001373328 1105 54-55 0.2550343751472255 1105 56-57 0.2131935820153643 1105 58-59 0.30239649746547315 1105 60-61 0.31427162995898783 1105 62-63 0.21535879854048545 1105 64-65 0.3264033198150216 1105 66-67 0.3288827489988151 1105 68-69 0.345144772744419 1105 70-71 0.2779263572823183 1105 72-73 0.14278787909950807 1105 74-75 0.1371216185389983 1105 76-77 0.1413178841978251 1105 78-79 0.19948181035112356 1105 80-81 0.2108653574381023 1105 82-83 0.2334635803349947 1105 84-85 0.26674916348505917 1105 86-87 0.2775410584329734 1105 88-89 0.22756935782639331 1105 90-91 0.30075755012871497 1105 92-93 0.28874438365672006 1105 94-95 0.24676480111137167 1105 96-97 0.29387846282554975 1105 98-99 0.35728084006382943 1105 100-101 0.36953747440635 1106 1 0.024164483839538065 1106 2 -0.04114738011887553 1106 3 0.024510981349475713 1106 4 -0.07380632356780126 1106 5 -0.02018485106333401 1106 6 -0.013445062702523103 1106 7 0.012976830064225453 1106 8 -0.06435207184172498 1106 9 -0.005246048841812012 1106 10-11 -3.90189444068767E-4 1106 12-13 -0.052202316886436506 1106 14-15 -0.021332973038468594 1106 16-17 0.038484910457171395 1106 18-19 -0.0020633096698432496 1106 20-21 0.07725980229769647 1106 22-23 0.03159081971296729 1106 24-25 0.012213569749185638 1106 26-27 -0.010797290117700697 1106 28-29 0.006191943741697514 1106 30-31 -0.00866770985508225 1106 32-33 0.013098734052462646 1106 34-35 0.053115693588260626 1106 36-37 0.04957471118400747 1106 38-39 0.0333907860892495 1106 40-41 0.13031239929978966 1106 42-43 0.038667583881888845 1106 44-45 -0.005869427138513572 1106 46-47 0.045897660384511596 1106 48-49 0.04715225543707646 1106 50-51 0.07312458999450655 1106 52-53 0.07156630626456462 1106 54-55 0.14348705992126298 1106 56-57 0.11365824978057049 1106 58-59 0.19259206881235258 1106 60-61 0.10900497863026004 1106 62-63 0.14554656560020618 1106 64-65 0.22087794718792253 1106 66-67 0.21811673933352438 1106 68-69 0.2880108684747711 1106 70-71 0.17399743823429503 1106 72-73 0.09022740800056539 1106 74-75 0.1910499480491552 1106 76-77 0.1366066931947998 1106 78-79 0.17444740260868485 1106 80-81 0.11136300947301692 1106 82-83 0.10215950842689736 1106 84-85 0.16499965020825513 1106 86-87 0.1138704629318319 1106 88-89 0.14501321945753176 1106 90-91 0.20714716659106003 1106 92-93 0.3284562317332629 1106 94-95 0.26742328951990046 1106 96-97 0.17500046828109106 1106 98-99 0.2042741634357128 1106 100-101 0.1732504733503326 1107 1 0.045848487963123574 1107 2 0.03727522004737338 1107 3 0.035628600303539315 1107 4 0.07482344493104875 1107 5 0.11675969844620937 1107 6 0.048708845235076126 1107 7 0.06062654770912701 1107 8 0.11027216649746663 1107 9 0.046608446172022866 1107 10-11 0.06339072262257872 1107 12-13 0.062280441073184534 1107 14-15 0.05335403928010152 1107 16-17 0.1132583159885101 1107 18-19 0.09900931168022709 1107 20-21 0.1653178284312986 1107 22-23 0.12265524799722982 1107 24-25 0.0973023461862681 1107 26-27 0.14528865463849883 1107 28-29 0.07581180863527948 1107 30-31 0.09303538491416674 1107 32-33 0.1515849950287702 1107 34-35 0.13939111195542608 1107 36-37 0.18867848748512017 1107 38-39 0.1068147392225498 1107 40-41 0.23437676062798118 1107 42-43 0.18539153621285465 1107 44-45 0.12461308046362518 1107 46-47 0.14799997096540096 1107 48-49 0.11074069542522835 1107 50-51 0.022993929801259583 1107 52-53 0.17635435053736614 1107 54-55 0.21161360206762225 1107 56-57 0.20957415309713667 1107 58-59 0.25516548174223175 1107 60-61 0.17290006393392332 1107 62-63 0.23629474537846562 1107 64-65 0.30300654459455956 1107 66-67 0.19826992673007737 1107 68-69 0.18716934497493298 1107 70-71 0.19561022609125445 1107 72-73 0.177616760962799 1107 74-75 0.24082422514547375 1107 76-77 0.20247316617405176 1107 78-79 0.21716951197911527 1107 80-81 0.28619976055995267 1107 82-83 0.33443650974067296 1107 84-85 0.3202097165387876 1107 86-87 0.3452480482402933 1107 88-89 0.2532809558403315 1107 90-91 0.3016775301043033 1107 92-93 0.2561512899278888 1107 94-95 0.3016675212685662 1107 96-97 0.368028449031538 1107 98-99 0.36351441012658725 1107 100-101 0.4370051887653723 1108 1 -0.02404028921545276 1108 2 0.04022459027114067 1108 3 0.06911540655060477 1108 4 0.06095824399258021 1108 5 0.04732751873395813 1108 6 0.00599680182693163 1108 7 0.012978391721098603 1108 8 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2316 76-77 -0.6917092090873993 2316 78-79 -0.7233769252440041 2316 80-81 -0.6697671853129279 2316 82-83 -0.6471237805147432 2316 84-85 -0.6627682628713423 2316 86-87 -0.6822326762772093 2316 88-89 -0.6481662982734662 2316 90-91 -0.6972091998591345 2316 92-93 -0.6653349168320553 2316 94-95 -0.674291524670636 2316 96-97 -0.6852918105513766 2316 98-99 -0.6493131393865959 2316 100-101 -0.7231958321057341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114710.0 3 17582.0 4 13497.0 5 11423.0 6 11243.0 7 11992.0 8 12244.0 9 12807.0 10 13212.0 11 13828.0 12 14666.0 13 16014.0 14 16999.0 15 19754.0 16 24083.0 17 30794.0 18 25803.0 19 18743.0 20 18971.0 21 19901.0 22 21584.0 23 24805.0 24 29538.0 25 33851.0 26 38251.0 27 44870.0 28 53788.0 29 65822.0 30 82184.0 31 102896.0 32 133958.0 33 179577.0 34 249618.0 35 367788.0 36 594751.0 37 1177632.0 38 1650813.0 39 2418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.05316712477754 26.257131233978164 14.004474729455726 22.685226911788575 2 13.071817742406658 21.93257173479017 45.94741470563671 19.048195817166466 3 15.422316747669583 32.541141054666674 31.32143023149035 20.715111966173396 4 9.812140590534057 19.20102971036373 45.55277964734839 25.434050051753832 5 10.539244168194827 39.026135065656526 38.56501360935621 11.869607156792442 6 23.43641176703997 43.7276968337676 21.606659348009696 11.229232051182732 7 22.14739598783919 33.71739906772151 29.01984162224771 15.115363322191591 8 21.70283481438513 39.02424415341971 22.923545227977424 16.34937580421774 9 24.87860917804932 15.923180554794508 25.421443161055173 33.776767106101005 10-11 21.272834836776536 29.081597465954527 32.245044646037236 17.400523051231698 12-13 21.531472276114975 27.043201917313947 32.11507473730275 19.310251069268332 14-15 19.67254266938247 28.655757028013607 29.062976553463315 22.6087237491406 16-17 17.84617620135073 30.6409071424934 30.859852894228716 20.653063761927154 18-19 17.432910264402178 31.412551634924057 32.58179060780548 18.572747492868285 20-21 18.030235752710013 29.603980039339355 31.637155851492988 20.728628356457644 22-23 18.45884956003031 31.009521182221334 30.495313099325262 20.0363161584231 24-25 18.105803634186934 31.017081449502758 31.600218652832513 19.2768962634778 26-27 18.615394498914483 30.03265997910215 31.427034186693774 19.924911335289593 28-29 18.244835528615507 31.54160391957539 30.070960110800183 20.142600441008916 30-31 19.784937296997022 30.49040040359685 29.5588966085394 20.16576569086673 32-33 19.80354885581427 31.127756541915687 29.549052322099005 19.519642280171038 34-35 19.957958661554944 30.015777311281628 30.988755595277013 19.03750843188642 36-37 19.661808136557827 30.39943995268696 31.564825854383923 18.373926056371296 38-39 20.188090113955646 29.56694587154056 32.039789056403414 18.205174958100372 40-41 19.190883306083574 29.827114385323412 31.79561601624669 19.186386292346327 42-43 19.734767261446276 29.953800807326193 30.853268412670715 19.458163518556816 44-45 18.77455827811485 31.938319685895898 30.627426894733954 18.659695141255302 46-47 20.054657678845057 29.930462683429855 30.423147194745614 19.591732442979477 48-49 19.989366034597722 29.2552509835278 30.790640172506972 19.964742809367504 50-51 19.94069897862662 29.629116247421045 29.262384249442274 21.167800524510056 52-53 18.269499524991513 30.774576711500135 30.04232410938279 20.91359965412556 54-55 19.126779569491344 31.441439793055203 29.514054959709906 19.917725677743547 56-57 19.935493244114753 30.14770138411941 30.16110198835967 19.755703383406168 58-59 20.163009880917418 29.205355735677287 30.989192032830942 19.642442350574356 60-61 19.615749175032565 31.503201142731967 29.494476024875414 19.386573657360053 62-63 19.884379963918242 30.20867618298882 28.73182908668736 21.175114766405574 64-65 18.940693939185227 30.310345556093143 29.054775283427013 21.694185221294614 66-67 19.587494882428956 28.573892800124582 30.182813562411066 21.655798755035395 68-69 18.93364830839018 30.047940443651534 29.297036700800433 21.72137454715785 70-71 19.753668526803896 29.07103137783392 30.36605515397185 20.809244941390332 72-73 20.517914356066047 28.79444099122051 30.907032775308434 19.780611877405 74-75 20.192899828554175 30.01696597323864 29.67346629331012 20.116667904897064 76-77 19.637773409487526 29.756593081020032 30.84302750472794 19.762606004764503 78-79 19.017099183800223 29.59988431440878 30.814089936064747 20.568926565726247 80-81 19.28945879147412 29.50246742288113 31.467382789712374 19.74069099593238 82-83 20.201793161062312 28.447224026872398 31.811485737114737 19.539497074950553 84-85 21.534031108026845 29.20769245054829 29.755943093057642 19.502333348367223 86-87 20.03565200168115 30.094209074106015 30.20315048208065 19.666988442132183 88-89 20.630466812685157 29.458046081646707 29.914194402885613 19.99729270278253 90-91 20.485099820690245 28.2454881006325 31.74559520142955 19.523816877247707 92-93 20.909576348378643 29.32035650268739 29.696673101800457 20.07339404713351 94-95 19.194298888248753 29.411577048710424 30.550553622624815 20.843570440416016 96-97 19.860447362509653 29.391677521412536 29.591756209252125 21.15611890682569 98-99 19.023761438181257 30.185073837057125 30.158993771255112 20.63217095350651 100-101 19.969117794286053 28.884873794202026 29.887003072891126 21.259005338620792 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 97.0 1 64.0 2 43.0 3 62.5 4 62.0 5 65.0 6 96.5 7 160.5 8 255.0 9 349.0 10 393.0 11 522.0 12 793.0 13 1090.0 14 1350.0 15 1668.5 16 2204.5 17 2721.0 18 3102.5 19 3494.5 20 3976.5 21 4535.0 22 5092.0 23 5774.5 24 6901.5 25 9099.0 26 14154.5 27 23725.0 28 45960.5 29 94736.5 30 154934.5 31 201704.0 32 252428.0 33 295926.5 34 342008.5 35 382712.0 36 386353.0 37 398035.0 38 366032.5 39 266797.5 40 189490.5 41 141067.5 42 112498.0 43 99487.0 44 89170.0 45 91317.5 46 96640.5 47 100397.5 48 104213.5 49 100900.0 50 94788.0 51 93346.5 52 90769.0 53 87410.0 54 83279.0 55 74016.5 56 66141.5 57 55633.5 58 44538.0 59 41118.0 60 35308.5 61 26900.5 62 20766.0 63 17232.5 64 13625.0 65 9062.0 66 6579.0 67 5128.0 68 4129.5 69 3402.5 70 2766.0 71 2154.5 72 1693.0 73 1380.5 74 1149.0 75 984.5 76 790.0 77 645.5 78 557.0 79 453.0 80 363.5 81 301.0 82 257.5 83 219.0 84 181.0 85 147.5 86 125.0 87 111.0 88 82.5 89 59.0 90 52.5 91 39.5 92 32.5 93 28.0 94 17.0 95 11.5 96 8.5 97 6.0 98 4.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.34584622128671E-4 2 0.003930156582728852 3 1.5115986856649427E-4 4 9.069592113989658E-4 5 0.0017950234392271197 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.668495071243536E-5 12-13 1.1336990142487072E-4 14-15 0.0033727545673899037 16-17 0.0043741886966429284 18-19 0.00391126159915804 20-21 0.0 22-23 0.0 24-25 3.778996714162357E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.7005485213730605E-4 42-43 2.834247535621768E-5 44-45 4.629270974848888E-4 46-47 0.7608254084623073 48-49 0.549588939632417 50-51 0.42228398782407256 52-53 0.3556035908026778 54-55 0.3466568160818984 56-57 0.3123057359501626 58-59 0.31240021086801667 60-61 0.28780838975060513 62-63 0.282498899367207 64-65 0.2782947655227014 66-67 0.2650399345477769 68-69 0.2425832465738671 70-71 0.2567450367601905 72-73 0.8407511889668411 74-75 0.5578365999610763 76-77 0.6531712395676073 78-79 0.3684049421719028 80-81 0.3205817387541781 82-83 0.30077034848018197 84-85 0.2918141262676172 86-87 0.2623473993889362 88-89 0.26609805362774236 90-91 0.2772177514591651 92-93 0.23205874072492494 94-95 0.19958771145848492 96-97 0.1556757696399183 98-99 0.1640273523782171 100-101 0.1598704559926385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5292410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.282190849068012 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68508974134241 22.45507433083896 2 11.692299187242714 6.847522725304877 3 4.391253573718933 3.8575657563686914 4 2.163698942018036 2.534313814403947 5 1.266067768717778 1.8536619015723839 6 0.7768366620261664 1.364848763760189 7 0.5220188467213817 1.0700098847554116 8 0.3598428879021073 0.8429590495383433 9 0.27015886521384586 0.7119759105683527 >10 1.4506171076576393 8.000256293171903 >50 0.17195704456315236 3.544363673730006 >100 0.19364991113891014 12.234119063401923 >500 0.027962776132839876 5.656238599372411 >1k 0.023940288609005173 14.406216237117842 >5k 0.0029844262222608075 5.755749824227331 >10k+ 0.00162197077296783 8.865124171867379 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 48227 0.91124837266954 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 36285 0.6856044788669057 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 32323 0.6107425539593494 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 28416 0.5369198531481877 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 26263 0.49623895352022995 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 23701 0.4478300056118101 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 23516 0.44433443365120995 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 18508 0.3497083559285845 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 17480 0.33028431281778997 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 17103 0.323160904011594 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 16774 0.3169444544167969 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 16589 0.3134488824561967 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 16142 0.30500282480004387 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 15412 0.29120948679335124 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 14552 0.2749598009224531 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 13475 0.2546099036166888 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 13205 0.2495082580525696 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 11902 0.22488809445980187 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 11836 0.2236410255441283 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 11790 0.22277185629987092 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 11294 0.2133999444487483 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 10959 0.20707012495252636 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 10438 0.19722583851213343 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 10226 0.19322010199512132 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 10220 0.19310673209369644 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 9667 0.1826578061790375 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 9534 0.18014477336411955 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 9435 0.1782741699906092 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9155 0.1729835745907819 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 9102 0.17198214046152885 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 9072 0.17141529095440453 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 8648 0.16340381792038033 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 8581 0.16213785402113592 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 8514 0.16087189012189154 No Hit ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG 7658 0.14469778418527665 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 7571 0.14305392061461603 No Hit CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT 7469 0.14112663229039324 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 7334 0.13857580950833365 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 7274 0.13744211049408492 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 6975 0.1317925104064122 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 6973 0.13175472043927058 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6576 0.12425341196165829 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 6551 0.12378103737238799 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 6272 0.11850933695613153 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 6246 0.1180180673832904 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 6205 0.11724337305688713 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6164 0.11646867873048383 No Hit CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA 6095 0.11516492486409782 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 6087 0.11501376499553134 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 5972 0.11284084188488798 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 5916 0.11178272280492252 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 5821 0.10998769936569541 No Hit GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT 5770 0.10902405520358399 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 5755 0.10874063045002182 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 5702 0.1077391963207688 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 5663 0.10700229196150715 No Hit GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC 5625 0.10628428258581629 No Hit TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT 5615 0.10609533275010817 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 5602 0.10584969796368764 No Hit AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT 5538 0.10464041901515567 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 5351 0.10110705708741385 No Hit CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG 5300 0.10014341292530246 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.8894983570811784E-5 0.0 4 0.0 0.0 0.0 1.8894983570811784E-5 0.0 5 0.0 0.0 0.0 1.8894983570811784E-5 0.0 6 0.0 0.0 0.0 1.8894983570811784E-5 0.0 7 0.0 0.0 0.0 1.8894983570811784E-5 0.0 8 0.0 0.0 0.0 1.8894983570811784E-5 0.0 9 1.8894983570811784E-5 0.0 0.0 1.8894983570811784E-5 0.0 10-11 3.778996714162357E-5 0.0 0.0 4.723745892702946E-5 0.0 12-13 3.778996714162357E-5 0.0 0.0 5.668495071243535E-5 0.0 14-15 4.723745892702946E-5 0.0 0.0 5.668495071243535E-5 0.0 16-17 5.668495071243535E-5 0.0 0.0 7.557993428324714E-5 0.0 18-19 5.668495071243535E-5 0.0 0.0 3.4010970427461216E-4 0.0 20-21 5.668495071243535E-5 0.0 0.0 3.590046878454239E-4 0.0 22-23 5.668495071243535E-5 0.0 0.0 3.684521796308298E-4 0.0 24-25 6.613244249784125E-5 0.0 0.0 4.723745892702946E-4 0.0 26-27 8.502742606865304E-5 0.0 0.0 7.652468346178773E-4 0.0 28-29 9.447491785405893E-5 0.0 0.0 0.001672206046016843 9.447491785405892E-6 30-31 9.447491785405893E-5 0.0 0.0 0.0066321392333549365 1.8894983570811784E-5 32-33 9.447491785405893E-5 0.0 0.0 0.02345812210316283 1.8894983570811784E-5 34-35 9.447491785405893E-5 0.0 0.0 0.053085456342195705 1.8894983570811784E-5 36-37 9.447491785405893E-5 9.447491785405892E-6 0.0 0.10151329923418631 3.778996714162357E-5 38-39 1.133699014248707E-4 1.8894983570811784E-5 0.0 0.16854325345164112 3.778996714162357E-5 40-41 1.133699014248707E-4 1.8894983570811784E-5 0.0 0.23796342309080362 3.778996714162357E-5 42-43 1.133699014248707E-4 3.778996714162357E-5 0.0 0.31531948582970704 3.778996714162357E-5 44-45 1.133699014248707E-4 3.778996714162357E-5 0.0 0.41544400377143875 3.778996714162357E-5 46-47 1.133699014248707E-4 3.778996714162357E-5 0.0 0.5388471414724105 3.778996714162357E-5 48-49 1.133699014248707E-4 3.778996714162357E-5 0.0 0.6673519247375015 3.778996714162357E-5 50-51 1.133699014248707E-4 3.778996714162357E-5 0.0 0.7948552738733393 3.778996714162357E-5 52-53 1.228173932102766E-4 3.778996714162357E-5 0.0 0.9247866283980266 3.778996714162357E-5 54-55 1.322648849956825E-4 3.778996714162357E-5 0.0 1.0723753450696374 3.778996714162357E-5 56-57 1.322648849956825E-4 3.778996714162357E-5 0.0 1.254107297053705 3.778996714162357E-5 58-59 1.322648849956825E-4 3.778996714162357E-5 0.0 1.4654098983260935 3.778996714162357E-5 60-61 1.322648849956825E-4 3.778996714162357E-5 0.0 1.6876715900695525 3.778996714162357E-5 62-63 1.322648849956825E-4 3.778996714162357E-5 0.0 1.900684187355099 3.778996714162357E-5 64-65 1.322648849956825E-4 3.778996714162357E-5 0.0 2.1269327206319995 3.778996714162357E-5 66-67 1.322648849956825E-4 3.778996714162357E-5 0.0 2.3945801629125483 3.778996714162357E-5 68-69 1.322648849956825E-4 3.778996714162357E-5 0.0 2.6972683522251675 3.778996714162357E-5 70-71 1.4171237678108837E-4 3.778996714162357E-5 0.0 3.0097630380110383 3.778996714162357E-5 72-73 1.5115986856649427E-4 3.778996714162357E-5 0.0 3.353207706885899 3.778996714162357E-5 74-75 1.5115986856649427E-4 3.778996714162357E-5 0.0 3.678465954073853 3.778996714162357E-5 76-77 1.5115986856649427E-4 3.778996714162357E-5 0.0 4.0460300694768545 3.778996714162357E-5 78-79 1.5115986856649427E-4 3.778996714162357E-5 0.0 4.447000515833052 3.778996714162357E-5 80-81 1.5115986856649427E-4 3.778996714162357E-5 0.0 4.919686872332265 4.723745892702946E-5 82-83 1.5115986856649427E-4 3.778996714162357E-5 0.0 5.42035669949985 5.668495071243535E-5 84-85 1.5115986856649427E-4 3.778996714162357E-5 0.0 5.8987776835128045 5.668495071243535E-5 86-87 1.5115986856649427E-4 3.778996714162357E-5 0.0 6.415564931666292 5.668495071243535E-5 88-89 1.5115986856649427E-4 3.778996714162357E-5 0.0 6.986221777980164 5.668495071243535E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4100 0.0 52.092102 2 ACCTGGG 4140 0.0 52.0434 3 CCTGGGG 3935 0.0 46.57156 4 GTACCTG 4695 0.0 45.994766 1 CTGGGGG 3855 0.0 43.85291 5 GTACCCG 515 0.0 42.298973 1 GTACACG 425 0.0 35.656628 1 TGGGGGG 9715 0.0 31.828844 6 CTGTGCG 2370 0.0 28.57292 9 TATTCTC 6685 0.0 28.051 5 GGGGGGA 9960 0.0 27.57523 7 GTACTAG 2770 0.0 27.524872 1 AGGGCTA 12305 0.0 27.515602 5 TCTCTGC 6780 0.0 27.098951 8 GGTATCA 7880 0.0 26.622984 1 CATGGGG 8645 0.0 26.292437 4 GTACATG 12760 0.0 25.942133 1 GTATCAA 11345 0.0 25.838385 1 GAACCGT 480 0.0 25.649717 6 GCTATTG 13180 0.0 25.616783 8 >>END_MODULE