##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931097_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1848769 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.7207368795128 33.0 33.0 33.0 27.0 33.0 2 30.610909745890375 33.0 33.0 33.0 27.0 33.0 3 30.664005616710362 33.0 33.0 33.0 27.0 33.0 4 34.19998442206679 37.0 37.0 37.0 27.0 37.0 5 34.14411319099357 37.0 37.0 37.0 27.0 37.0 6 34.25205690921905 37.0 37.0 37.0 27.0 37.0 7 34.21026802158625 37.0 37.0 37.0 27.0 37.0 8 34.20853173111406 37.0 37.0 37.0 27.0 37.0 9 34.2344278814714 37.0 37.0 37.0 27.0 37.0 10-11 34.11893157014208 37.0 37.0 37.0 27.0 37.0 12-13 33.989389696603524 37.0 37.0 37.0 27.0 37.0 14-15 35.501318174417676 40.0 37.0 40.0 27.0 40.0 16-17 35.40689020640221 40.0 37.0 40.0 27.0 40.0 18-19 35.2962633514517 40.0 37.0 40.0 27.0 40.0 20-21 35.185121018364114 40.0 37.0 40.0 27.0 40.0 22-23 35.046058755853224 37.0 37.0 40.0 27.0 40.0 24-25 34.8879119024605 37.0 37.0 40.0 27.0 40.0 26-27 34.684030563039514 37.0 37.0 40.0 22.0 40.0 28-29 34.53012166474016 37.0 37.0 40.0 22.0 40.0 30-31 34.37842531976683 37.0 35.0 40.0 22.0 40.0 32-33 34.22416672932097 37.0 33.0 40.0 22.0 40.0 34-35 34.07789696819884 37.0 33.0 40.0 22.0 40.0 36-37 33.831550886022 37.0 33.0 40.0 18.5 40.0 38-39 33.59995867520496 37.0 33.0 40.0 15.0 40.0 40-41 33.34211872873247 37.0 33.0 40.0 15.0 40.0 42-43 33.11120156168781 37.0 33.0 40.0 15.0 40.0 44-45 32.78290283967332 37.0 33.0 40.0 15.0 40.0 46-47 32.689634562241146 37.0 33.0 40.0 6.0 40.0 48-49 32.64186331553591 37.0 33.0 40.0 6.0 40.0 50-51 31.807327740783194 37.0 33.0 38.5 6.0 40.0 52-53 31.934566460168902 37.0 33.0 37.0 6.0 40.0 54-55 32.33882518583987 37.0 33.0 40.0 6.0 40.0 56-57 32.22411020522304 37.0 33.0 40.0 6.0 40.0 58-59 31.98369861242805 37.0 33.0 40.0 2.0 40.0 60-61 31.983114439932734 37.0 33.0 40.0 2.0 40.0 62-63 31.764737238670705 37.0 33.0 37.0 2.0 40.0 64-65 31.49556677984107 37.0 33.0 37.0 2.0 40.0 66-67 31.198495593554412 37.0 33.0 37.0 2.0 40.0 68-69 30.898245805722617 37.0 33.0 37.0 2.0 40.0 70-71 30.65872994408712 37.0 33.0 37.0 2.0 40.0 72-73 30.082558177901078 37.0 27.0 37.0 2.0 40.0 74-75 29.909034876720675 35.0 27.0 37.0 2.0 37.0 76-77 29.600575301727797 33.0 27.0 37.0 2.0 37.0 78-79 29.352494010879674 33.0 27.0 37.0 2.0 37.0 80-81 29.108269069851346 33.0 27.0 37.0 2.0 37.0 82-83 28.841677624408458 33.0 27.0 37.0 2.0 37.0 84-85 28.467884305719103 33.0 27.0 37.0 2.0 37.0 86-87 28.29185366046272 33.0 27.0 37.0 2.0 37.0 88-89 28.12355248276015 33.0 27.0 37.0 2.0 37.0 90-91 27.805485433821097 33.0 27.0 37.0 2.0 37.0 92-93 27.489268264450562 33.0 27.0 37.0 2.0 37.0 94-95 27.204308921233533 33.0 27.0 37.0 2.0 37.0 96-97 26.855358349258346 33.0 22.0 37.0 2.0 37.0 98-99 26.463908146447714 33.0 22.0 37.0 2.0 37.0 100-101 25.68635291915864 33.0 18.5 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13599899766666113 1101 2 -0.005614646419829228 1101 3 -0.031194853928123933 1101 4 -0.06260637240296063 1101 5 -0.01845058303922542 1101 6 -0.027462440510547026 1101 7 0.10317694228047003 1101 8 -0.03800801562118039 1101 9 0.11856146334314843 1101 10-11 0.002219193810070408 1101 12-13 -0.09046232153542633 1101 14-15 0.03668074909126773 1101 16-17 0.2254914419831664 1101 18-19 0.042177158327483255 1101 20-21 0.2454288831575795 1101 22-23 0.09047897987012732 1101 24-25 0.1399721909110525 1101 26-27 0.12862440774254225 1101 28-29 0.2823262420557384 1101 30-31 0.13547689258358986 1101 32-33 0.02871056356023871 1101 34-35 0.1763601766636853 1101 36-37 0.17076985056895921 1101 38-39 0.23289053295680873 1101 40-41 0.0941768247421777 1101 42-43 0.05610807551792618 1101 44-45 0.11201178909038134 1101 46-47 0.28891088254238895 1101 48-49 0.2839241365695173 1101 50-51 0.4345557202075554 1101 52-53 0.4724641892529746 1101 54-55 0.467524726658219 1101 56-57 0.3868382523540532 1101 58-59 0.43386709848389415 1101 60-61 0.5171447872733026 1101 62-63 0.36649614169677847 1101 64-65 0.3635438961127839 1101 66-67 0.30167395399730523 1101 68-69 0.573744182526795 1101 70-71 0.40374867462690744 1101 72-73 0.6266475960474125 1101 74-75 0.496387553124066 1101 76-77 0.374093212236172 1101 78-79 0.29431518968086934 1101 80-81 0.5350491339164414 1101 82-83 0.43927313184574146 1101 84-85 0.4469823524491652 1101 86-87 0.30731461283197703 1101 88-89 0.5003439463334445 1101 90-91 0.4814398723070674 1101 92-93 0.3411935651293554 1101 94-95 0.3124566554897079 1101 96-97 0.23408487937542688 1101 98-99 0.34975616496914697 1101 100-101 0.3976079524886771 1102 1 0.019609757463296518 1102 2 -0.025602486517108503 1102 3 -0.030396860312070118 1102 4 0.09425637269507803 1102 5 0.09418051591198662 1102 6 -0.06823611432115939 1102 7 0.11408285503316762 1102 8 0.14218654282235121 1102 9 0.0490980190507031 1102 10-11 0.08965649431166867 1102 12-13 0.12782693215054053 1102 14-15 0.18066159724448738 1102 16-17 0.10645739425554979 1102 18-19 0.1754230923600062 1102 20-21 0.19262830556220223 1102 22-23 0.1716273306833216 1102 24-25 0.22047454689220558 1102 26-27 0.008678367310871238 1102 28-29 -0.06434498457445414 1102 30-31 0.21195128078848313 1102 32-33 0.14033467056737692 1102 34-35 0.11485766868374725 1102 36-37 0.15543697323197136 1102 38-39 0.1473722171033458 1102 40-41 -0.08311075695716141 1102 42-43 0.002718502634508013 1102 44-45 -0.04241897546350515 1102 46-47 0.1312881435243085 1102 48-49 -0.032613331130740164 1102 50-51 -0.024993603397859943 1102 52-53 0.16318466348917582 1102 54-55 0.12733244819644796 1102 56-57 0.2932640009480707 1102 58-59 0.38129451906452516 1102 60-61 0.439891405300358 1102 62-63 0.3486362845756332 1102 64-65 0.2260989956719861 1102 66-67 0.3304587237191505 1102 68-69 0.30316734714147486 1102 70-71 0.22108413594321874 1102 72-73 0.28702531302567635 1102 74-75 0.17090115701523345 1102 76-77 0.18724870699221086 1102 78-79 0.015606419351033907 1102 80-81 0.10352458611282245 1102 82-83 0.017723504242763255 1102 84-85 0.16173763440691147 1102 86-87 -0.13705383222046308 1102 88-89 -0.1914975189348347 1102 90-91 -0.057490816247419474 1102 92-93 -0.1511874958221533 1102 94-95 -0.2801486036682199 1102 96-97 -0.37372025818347154 1102 98-99 -0.45925736292263153 1102 100-101 -0.387864763729592 1103 1 0.3241106760181047 1103 2 0.2820358386592048 1103 3 0.1783085233210393 1103 4 0.19630377676899968 1103 5 0.2790639994895585 1103 6 0.3173009593736822 1103 7 0.46024470423193975 1103 8 0.36681077399751416 1103 9 0.30735986967358997 1103 10-11 0.36434235806174087 1103 12-13 0.3864200875326773 1103 14-15 0.46494006888321593 1103 16-17 0.3994951681407386 1103 18-19 0.438019200409272 1103 20-21 0.5696770546345462 1103 22-23 0.4067118915255179 1103 24-25 0.4659389352897705 1103 26-27 0.3699004407920867 1103 28-29 0.37391009358577776 1103 30-31 0.5070741978210691 1103 32-33 0.35564340308833664 1103 34-35 0.34469277978852375 1103 36-37 0.452807224398569 1103 38-39 0.5406748765608214 1103 40-41 0.4163372003066925 1103 42-43 0.3135515946593017 1103 44-45 0.3758324158186852 1103 46-47 0.39791984023189997 1103 48-49 0.49148904065530274 1103 50-51 0.5585650171717518 1103 52-53 0.6836871865479921 1103 54-55 0.8227660598653372 1103 56-57 0.7701436758065654 1103 58-59 0.6611639671879388 1103 60-61 0.6055354775758275 1103 62-63 0.6918877945341677 1103 64-65 0.6647425410245411 1103 66-67 0.6939827224469362 1103 68-69 0.6853745607636199 1103 70-71 0.6732251360840458 1103 72-73 0.40114071547294117 1103 74-75 0.6759482636071503 1103 76-77 0.7127534374307949 1103 78-79 0.5204616658734231 1103 80-81 0.5085142929189885 1103 82-83 0.5921800457612783 1103 84-85 0.5552717127111357 1103 86-87 0.3427510164197436 1103 88-89 0.4683335888216966 1103 90-91 0.27401905524984826 1103 92-93 0.228177815244333 1103 94-95 0.2131505350580838 1103 96-97 0.4173908054571065 1103 98-99 0.3590104056933363 1103 100-101 0.37511466887095324 1104 1 0.06915930701284623 1104 2 0.1383623504128444 1104 3 0.1441395947476387 1104 4 0.1338410154773939 1104 5 -0.06773285094324422 1104 6 0.035908728195778394 1104 7 0.026790620938605514 1104 8 0.14068940615079129 1104 9 -0.06619023431895243 1104 10-11 -0.038237387942686496 1104 12-13 -0.036177188248927905 1104 14-15 0.08708182561238687 1104 16-17 0.08572008718103064 1104 18-19 0.06327969896661045 1104 20-21 0.07234013948914964 1104 22-23 0.039331517428209395 1104 24-25 0.18137003540396535 1104 26-27 0.17056991001636135 1104 28-29 0.06691418156843554 1104 30-31 -0.024072533723696665 1104 32-33 -0.11066487549030768 1104 34-35 0.0322969102508992 1104 36-37 -0.03689489351849318 1104 38-39 0.09396415788133083 1104 40-41 0.15715130743280525 1104 42-43 0.210045742665244 1104 44-45 -0.061205203552056275 1104 46-47 -0.009597655064986554 1104 48-49 0.03707990862064037 1104 50-51 -0.037219492077234406 1104 52-53 0.08177622016213348 1104 54-55 0.1225704187251182 1104 56-57 0.0821633654288263 1104 58-59 0.14061561341469542 1104 60-61 -0.07283207399288827 1104 62-63 -0.057674823043608825 1104 64-65 -0.01931356805453177 1104 66-67 -0.03452399155426633 1104 68-69 0.16047669136942488 1104 70-71 0.1112235486117008 1104 72-73 -0.02588934581921265 1104 74-75 0.06589426058392078 1104 76-77 -0.03432970281294345 1104 78-79 0.02608419363694736 1104 80-81 0.055070608879773886 1104 82-83 -0.0839792159192605 1104 84-85 0.1645442200623357 1104 86-87 0.07192113111496568 1104 88-89 -0.18987816702478355 1104 90-91 0.04775614583093457 1104 92-93 0.10436238545446841 1104 94-95 -0.0024319335096869565 1104 96-97 0.2383467506858601 1104 98-99 0.08744195267203381 1104 100-101 0.2828066344127329 1105 1 -0.0025698701380179045 1105 2 -0.06754049931530659 1105 3 -0.010399322592483884 1105 4 0.03445267587821377 1105 5 -0.08159234296538642 1105 6 0.05460266857646445 1105 7 -0.005809681993021343 1105 8 0.014582398266675511 1105 9 0.1270773075931686 1105 10-11 0.05894803680298111 1105 12-13 0.08978859133131323 1105 14-15 0.052219711307500916 1105 16-17 -0.05030743815372318 1105 18-19 -0.07313173300204312 1105 20-21 0.0607091560961166 1105 22-23 0.040648517615693436 1105 24-25 -0.0018117087717470781 1105 26-27 -0.056118169081351255 1105 28-29 -0.13202161074085694 1105 30-31 -0.14904909845930092 1105 32-33 -0.05928799203510948 1105 34-35 0.045307799014793204 1105 36-37 -0.19269869841868115 1105 38-39 -0.07163312066776228 1105 40-41 -0.08143633552230511 1105 42-43 -0.26074620892489975 1105 44-45 -0.0018819153131062194 1105 46-47 -0.15946401238615948 1105 48-49 -0.16490925195285655 1105 50-51 -0.18262235660660764 1105 52-53 -0.017508510899705954 1105 54-55 0.16886184980570107 1105 56-57 0.09451374976716664 1105 58-59 0.22612380095601026 1105 60-61 0.13777879700954543 1105 62-63 0.08404161272402888 1105 64-65 0.09202428951826747 1105 66-67 -0.02210268470524923 1105 68-69 0.08695471649203057 1105 70-71 0.12321839532040357 1105 72-73 -0.07422766222981991 1105 74-75 -0.013186497337198233 1105 76-77 -0.11686839566790042 1105 78-79 -0.14344805081999468 1105 80-81 -0.31975486007220155 1105 82-83 -0.30115843648703944 1105 84-85 -0.20899724308756262 1105 86-87 0.006560795423304455 1105 88-89 -0.13834587076356897 1105 90-91 -0.03870425350596918 1105 92-93 -0.09498629921047907 1105 94-95 -0.20681580807428546 1105 96-97 -0.32401757146761767 1105 98-99 -0.18297817994689325 1105 100-101 -0.07993148020791097 1106 1 0.0784583110651127 1106 2 -0.053427532209425976 1106 3 -0.023921156020143997 1106 4 0.00879598320746311 1106 5 0.04861424629579858 1106 6 0.022218918136140076 1106 7 0.07344820450061462 1106 8 0.03901644135389404 1106 9 -0.10965914991633952 1106 10-11 0.008450217132612181 1106 12-13 -0.043416844240709906 1106 14-15 0.06537515368237479 1106 16-17 -0.02092175744294167 1106 18-19 0.025119002109804 1106 20-21 0.10627770368408562 1106 22-23 0.11623721282865773 1106 24-25 0.04604806425528096 1106 26-27 0.11168303107843514 1106 28-29 0.011756357834322273 1106 30-31 0.023927394735665075 1106 32-33 0.06431998600192657 1106 34-35 0.09044379082403253 1106 36-37 0.15073381573528621 1106 38-39 0.18873952031125185 1106 40-41 0.20787840293443338 1106 42-43 0.0779375106385487 1106 44-45 0.2138519011987512 1106 46-47 0.14568910148737046 1106 48-49 0.058172576728338754 1106 50-51 0.1915958590083946 1106 52-53 0.0970095831337261 1106 54-55 0.251324066019599 1106 56-57 0.1096202749759243 1106 58-59 0.1918998634900646 1106 60-61 0.03866559071663289 1106 62-63 0.31869993870054714 1106 64-65 0.3372101067830968 1106 66-67 0.33554002453215404 1106 68-69 0.27709909227288065 1106 70-71 0.3813853901185489 1106 72-73 0.10646975747011922 1106 74-75 0.1708144991719145 1106 76-77 0.13199251489540487 1106 78-79 0.24777853758384438 1106 80-81 0.2527178803713461 1106 82-83 0.3705233403806538 1106 84-85 0.39524901021834324 1106 86-87 0.3749049501580579 1106 88-89 0.34626166679656123 1106 90-91 0.3939886694776682 1106 92-93 0.5320780868336712 1106 94-95 0.6676833746515634 1106 96-97 0.7584250748977652 1106 98-99 0.7520617270124568 1106 100-101 0.7347088166573741 1107 1 -0.017292529438989845 1107 2 -0.002186852636356207 1107 3 0.12755089065893443 1107 4 0.0892724084087888 1107 5 0.0893617436513523 1107 6 -0.007230564943512263 1107 7 0.1036703853183738 1107 8 -0.1166304136690286 1107 9 -0.09265419828291499 1107 10-11 -0.08860987581517321 1107 12-13 -0.03227905935079889 1107 14-15 -0.06609298381242468 1107 16-17 -0.10325253929159572 1107 18-19 0.025359567296483476 1107 20-21 0.048366646765657606 1107 22-23 0.045027116873811224 1107 24-25 -0.07031915378521347 1107 26-27 -0.16285004840359107 1107 28-29 -0.1659340512797982 1107 30-31 -0.06036844501961269 1107 32-33 -0.09468254700797729 1107 34-35 -0.1474934570394737 1107 36-37 -0.030051511675111442 1107 38-39 -0.2798230909059214 1107 40-41 -0.025497687716189432 1107 42-43 -0.11053204666254857 1107 44-45 -0.06796196030881418 1107 46-47 -0.2583199350372638 1107 48-49 -0.16488648084574464 1107 50-51 -0.3313549737127204 1107 52-53 -0.12833292744701197 1107 54-55 -0.00888315022844921 1107 56-57 -0.15804799978253925 1107 58-59 -0.11031560626652848 1107 60-61 -0.21390102756184248 1107 62-63 0.032653486034696755 1107 64-65 0.05418781794685401 1107 66-67 -0.13072115025142494 1107 68-69 -0.19481610142336692 1107 70-71 -0.27531924418109455 1107 72-73 -0.1301859501158198 1107 74-75 0.048114461554121135 1107 76-77 -0.0527591899924289 1107 78-79 -0.030243768941016214 1107 80-81 0.16010352641269066 1107 82-83 0.19584147015142506 1107 84-85 0.19603243905055123 1107 86-87 0.2781321498259821 1107 88-89 0.2608754712288537 1107 90-91 0.22089803772282934 1107 92-93 0.2593563216984087 1107 94-95 0.16562073454297987 1107 96-97 0.1819971318362441 1107 98-99 0.16293571566579956 1107 100-101 0.09563147848757936 1108 1 -0.10464766827927718 1108 2 -0.2296255632433848 1108 3 -0.16823552789976048 1108 4 -0.16978154272437251 1108 5 -0.22912902434436688 1108 6 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-0.8967827874009338 2316 98-99 -0.789871867111966 2316 100-101 -0.9043793711753558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69266.0 3 10408.0 4 8262.0 5 7054.0 6 7255.0 7 7634.0 8 7800.0 9 8176.0 10 8019.0 11 8048.0 12 8377.0 13 8220.0 14 8654.0 15 9090.0 16 10300.0 17 11887.0 18 10787.0 19 9764.0 20 10112.0 21 10884.0 22 11974.0 23 13696.0 24 16010.0 25 18389.0 26 21040.0 27 25180.0 28 29900.0 29 36313.0 30 44665.0 31 55023.0 32 70693.0 33 91123.0 34 124511.0 35 178536.0 36 268592.0 37 379729.0 38 223331.0 39 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22430854893315 20.273243049248766 11.426812717950494 24.075635683867596 2 16.09308559982734 19.952810261442185 39.9678261617444 23.986277976986077 3 19.28764298742295 27.401442260883485 27.881168175061827 25.42974657663174 4 12.864896383706991 16.415090461415964 39.078909789565245 31.6411033653118 5 13.441933601155817 37.9737085372338 34.266150350212065 14.318207511398324 6 29.554746969469953 37.85908353071692 18.613304312220727 13.972865187592392 7 27.20756351929311 30.870811875361387 24.069799958783385 17.851824646562118 8 26.393778779285025 34.66209136998727 19.809722036663317 19.134407814064385 9 26.775708593123316 14.534211683558087 21.44600001406341 37.24407970925518 10-11 26.18560035656178 25.145475257036036 27.33233699414962 21.33658739225257 12-13 26.20142170353446 23.04285881192232 27.360166337799008 23.39555314674421 14-15 23.54995235858153 24.2786780919524 24.863032612261602 27.308336937204476 16-17 22.691045678733204 26.60534950619194 26.151164010917977 24.55244080415688 18-19 22.098777786492636 27.738729211687165 27.492041683457792 22.67045131836241 20-21 23.51530221333823 26.695662545094205 26.666129372065782 23.12290586950178 22-23 24.091138481876317 26.637075805576576 26.147209305218773 23.124576407328334 24-25 23.82210747914287 26.316227887977288 26.8659182763873 22.99574635649254 26-27 23.82068825256157 26.368545772889963 25.841654636138966 23.969111338409505 28-29 22.89120490445264 27.09018811977051 26.575223838132295 23.443383137644563 30-31 23.755509747296717 26.729786144185674 26.468260772438306 23.046443336079307 32-33 23.92143096298131 26.763835828056397 25.737098577485884 23.577634631476403 34-35 23.89938926929216 26.440458488864753 26.652518513670447 23.00763372817264 36-37 23.875400333951944 26.081841484793394 25.993593574967992 24.04916460628667 38-39 24.24556556281504 26.086385048645884 26.57925354654908 23.088795841989995 40-41 23.528051556565718 25.4203142583524 27.22054257286822 23.831091612213665 42-43 23.873865224337173 25.663434875702396 26.39083800919369 24.071861890766748 44-45 23.831006683129957 26.332657114135184 26.426584961429544 23.40975124130532 46-47 24.02559247538965 26.10385112406818 25.812780009595272 24.0577763909469 48-49 24.61240352212197 25.655886509403196 26.148385694097186 23.583324274377652 50-51 23.766038030872938 25.829943666009363 26.22815266207424 24.17586564104346 52-53 23.58320414936904 26.12213704008564 26.12585384857885 24.16880496196647 54-55 23.278521044804542 26.20355816897152 26.092714656897826 24.42520612932611 56-57 24.501100379268124 25.452430122865515 25.89697521129587 24.14949428657049 58-59 23.27245153990764 25.84575322642408 26.92167087382748 23.960124359840798 60-61 23.479667945613443 26.922656662846368 25.877808388990704 23.71986700254949 62-63 23.868248825974135 26.112894641735373 25.245955063704173 24.77290146858632 64-65 22.864773507336842 26.43109354785187 26.002980066737013 24.70115287807428 66-67 23.695841461338528 25.72227649928488 26.076203911635254 24.505678127741337 68-69 23.07976892775388 25.895318223039897 26.161581447437676 24.863331401768544 70-71 22.992706072165106 25.832127979320678 26.138625831368984 25.03654011714523 72-73 23.896726662416288 25.556908774855184 26.560270934484205 23.98609362824432 74-75 23.978120994909357 26.16767925141561 25.845016376370634 24.009183377304407 76-77 23.698980771480098 25.891750151646626 25.796246780059352 24.613022296813924 78-79 23.67612386148741 26.12315606406329 25.46244570275088 24.738274371698417 80-81 23.257005971004947 26.6137266669903 26.334689241695656 23.794578120309094 82-83 24.23627645811997 25.44643353450195 27.2046184995408 23.11267150783728 84-85 24.375487185744014 25.75667486833276 26.03924598897859 23.828591956944642 86-87 23.093742096536044 26.569544925830442 26.425930599420393 23.910782378213128 88-89 24.281795714696585 25.230636696342966 26.265597947643105 24.221969641317344 90-91 23.777444683646877 25.44724393524238 26.156067522861363 24.61924385824938 92-93 24.2001785857042 25.680000975581997 26.189905165300175 23.92991527341363 94-95 23.101189176419336 25.675715215357048 27.03432502710555 24.188770581118067 96-97 23.492036324747552 25.673221886383473 26.235863877252186 24.598877911616785 98-99 22.908849127301927 26.21122150646551 26.100477075848694 24.77945229038387 100-101 23.277430040991774 25.62346172512111 25.702975370269264 25.396132863617847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21.0 1 15.0 2 13.5 3 17.0 4 18.5 5 18.0 6 17.5 7 35.0 8 56.0 9 70.0 10 75.5 11 74.0 12 90.5 13 132.5 14 179.5 15 238.5 16 313.5 17 389.0 18 458.5 19 541.5 20 603.0 21 693.5 22 843.0 23 957.0 24 1037.0 25 1111.5 26 1296.5 27 1689.5 28 2577.5 29 4926.5 30 8317.0 31 11911.5 32 16877.0 33 23417.0 34 32459.5 35 39734.0 36 45517.0 37 55305.5 38 58822.0 39 54483.5 40 49048.0 41 44474.0 42 43750.0 43 46776.5 44 51708.5 45 60005.5 46 70790.5 47 80724.0 48 89166.0 49 89865.0 50 85828.5 51 86700.5 52 86479.5 53 84161.0 54 81212.0 55 72250.0 56 64491.5 57 54487.0 58 43964.0 59 41885.0 60 36229.5 61 27252.0 62 21345.0 63 17784.5 64 13934.5 65 8973.5 66 6149.5 67 4642.5 68 3671.0 69 2887.0 70 2353.0 71 1913.0 72 1551.5 73 1298.0 74 1067.5 75 882.5 76 712.0 77 578.5 78 457.0 79 358.5 80 302.0 81 248.0 82 202.5 83 159.5 84 134.5 85 114.0 86 99.5 87 85.5 88 64.0 89 47.5 90 40.0 91 36.0 92 25.5 93 20.0 94 12.5 95 8.0 96 9.0 97 5.5 98 3.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.490805503553986E-4 2 0.003353582843502893 3 2.7045022931474947E-4 4 0.0013522511465737471 5 0.0017308814676143963 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.4090045862949884E-5 12-13 5.4090045862949884E-5 14-15 0.0033806278664343676 16-17 0.004056753439721242 18-19 0.0040026633938582915 20-21 2.7045022931474942E-5 22-23 0.0 24-25 5.4090045862949884E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.893151605203246E-4 42-43 2.7045022931474942E-5 44-45 5.138554356980239E-4 46-47 0.6733128909020002 48-49 0.4851336213447976 50-51 0.3730590463167654 52-53 0.31318136554647985 54-55 0.30452695820840786 56-57 0.2751290182818946 58-59 0.27515606330482606 60-61 0.25278982934049626 62-63 0.24775945507524194 64-65 0.24421655707121873 66-67 0.23383126826553238 68-69 0.21517020244281468 70-71 0.22831408358751146 72-73 0.7377341355247735 74-75 0.4826454792351018 76-77 0.5741117467893502 78-79 0.3193476307748561 80-81 0.2763730893367425 82-83 0.2592806348440503 84-85 0.2505180474142524 86-87 0.22963928971115374 88-89 0.23018019016978322 90-91 0.2404572988837437 92-93 0.20102565544965326 94-95 0.17341268703661733 96-97 0.13411626871718427 98-99 0.14206750545903787 100-101 0.13765916672120745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1848769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.189482795597215 #Duplication Level Percentage of deduplicated Percentage of total 1 85.79018049165789 32.76282621917348 2 7.838462713935454 5.986936739155366 3 2.1752656959280032 2.4921681561148583 4 0.9986570104356324 1.5255277887493652 5 0.5508531071477738 1.051839762916059 6 0.343708043492715 0.7875619448204052 7 0.2619763073618127 0.7003317777993625 8 0.19398587717465507 0.5926576255160261 9 0.14852289400397678 0.5104811254785463 >10 1.1728895889765647 9.35894659878895 >50 0.21550456258957384 5.842149427329427 >100 0.26444828938422843 21.52481024908778 >500 0.03330508684800689 8.40978603643509 >1k 0.011955672201848629 7.7260560819863 >5k 2.8465886194877686E-4 0.7279204666490353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 7711 0.4170883436492066 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 5681 0.3072855505474183 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 4215 0.22798954331233376 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 4085 0.2209578373501503 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 3410 0.18444705639265913 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 3264 0.17654990969666842 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2959 0.16005244570846872 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 2878 0.15567115199356976 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 2837 0.15345346011318883 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 2719 0.14707083470136073 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 2713 0.14674629442618306 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 2685 0.14523177314202043 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 2594 0.14030957896849203 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 2575 0.13928186809709597 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2517 0.13614464543704488 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 2473 0.13376468341907505 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 2360 0.12765250823656174 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2262 0.12235168374199264 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 2090 0.11304819585356525 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 2021 0.1093159826890217 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 2010 0.10872099218452928 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 2001 0.10823418177176272 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 1979 0.10704420076277782 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 1951 0.10552967947861523 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 1937 0.10477241883653393 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 1930 0.10439378851549329 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 1913 0.10347425773582312 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 1893 0.10239245681856414 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 1889 0.10217609663511233 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 1860 0.10060748530508679 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4090045862949884E-5 2 0.0 0.0 0.0 0.0 5.4090045862949884E-5 3 0.0 0.0 0.0 0.0 5.4090045862949884E-5 4 0.0 0.0 0.0 0.0 5.4090045862949884E-5 5 0.0 0.0 0.0 0.0 5.4090045862949884E-5 6 0.0 0.0 0.0 0.0 5.4090045862949884E-5 7 0.0 0.0 0.0 0.0 5.4090045862949884E-5 8 0.0 0.0 0.0 0.0 5.4090045862949884E-5 9 0.0 0.0 0.0 0.0 5.4090045862949884E-5 10-11 0.0 0.0 0.0 0.0 5.4090045862949884E-5 12-13 0.0 0.0 0.0 2.7045022931474942E-5 1.6227013758884966E-4 14-15 0.0 5.4090045862949884E-5 0.0 8.113506879442483E-5 2.434052063832745E-4 16-17 0.0 1.0818009172589977E-4 0.0 1.0818009172589977E-4 4.327203669035991E-4 18-19 0.0 1.0818009172589977E-4 0.0 1.6227013758884966E-4 4.86810412766549E-4 20-21 0.0 1.0818009172589977E-4 0.0 1.6227013758884966E-4 6.490805503553986E-4 22-23 0.0 1.0818009172589977E-4 0.0 1.6227013758884966E-4 7.031705962183485E-4 24-25 0.0 1.0818009172589977E-4 0.0 2.974952522462244E-4 7.572606420812984E-4 26-27 0.0 1.0818009172589977E-4 0.0 7.031705962183485E-4 8.654407338071981E-4 28-29 0.0 1.0818009172589977E-4 0.0 0.0017849715134773464 8.654407338071981E-4 30-31 0.0 1.0818009172589977E-4 0.0 0.006923525870457586 8.654407338071981E-4 32-33 0.0 1.0818009172589977E-4 0.0 0.022907134422959278 9.195307796701481E-4 34-35 0.0 1.0818009172589977E-4 0.0 0.04938421187287324 9.195307796701481E-4 36-37 2.7045022931474942E-5 1.0818009172589977E-4 0.0 0.09473871532895672 9.195307796701481E-4 38-39 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.1583486092637858 9.195307796701481E-4 40-41 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.2233377993681201 9.195307796701481E-4 42-43 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.29162648227009436 9.195307796701481E-4 44-45 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.3710577146198362 9.46575802601623E-4 46-47 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.47361244157598925 9.73620825533098E-4 48-49 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.589311049676839 9.73620825533098E-4 50-51 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.7120413637398724 9.73620825533098E-4 52-53 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.8292004030790218 0.0010547558943275227 54-55 5.4090045862949884E-5 1.0818009172589977E-4 0.0 0.9546352194352026 0.0010818009172589977 56-57 5.4090045862949884E-5 1.0818009172589977E-4 0.0 1.1156342409462727 0.0011358909631219477 58-59 5.4090045862949884E-5 1.0818009172589977E-4 0.0 1.2958352287386905 0.0011358909631219477 60-61 5.4090045862949884E-5 1.0818009172589977E-4 0.0 1.4865026404055888 0.0011358909631219477 62-63 5.4090045862949884E-5 1.0818009172589977E-4 0.0 1.6839583528282875 0.0011899810089848976 64-65 5.4090045862949884E-5 1.0818009172589977E-4 0.0 1.8938818208223958 0.0012440710548478474 66-67 5.4090045862949884E-5 1.0818009172589977E-4 0.0 2.1277671791337913 0.0012440710548478474 68-69 5.4090045862949884E-5 1.0818009172589977E-4 0.0 2.3834778709508866 0.0012440710548478474 70-71 5.4090045862949884E-5 1.0818009172589977E-4 0.0 2.662501372534914 0.0012440710548478474 72-73 5.4090045862949884E-5 1.0818009172589977E-4 0.0 2.9564266817541833 0.0013252061236422722 74-75 5.4090045862949884E-5 1.0818009172589977E-4 0.0 3.239777387007247 0.001379296169505222 76-77 5.4090045862949884E-5 1.6227013758884966E-4 0.0 3.5685096407393244 0.001406341192436697 78-79 5.4090045862949884E-5 1.6227013758884966E-4 0.0 3.9262882490998066 0.001406341192436697 80-81 5.4090045862949884E-5 1.6227013758884966E-4 0.0 4.3251482472932 0.001406341192436697 82-83 5.4090045862949884E-5 1.6227013758884966E-4 0.0 4.734096039040032 0.0015145212841625969 84-85 5.4090045862949884E-5 1.6227013758884966E-4 0.0 5.141772714709084 0.0015145212841625969 86-87 5.4090045862949884E-5 1.6227013758884966E-4 0.0 5.584986550510096 0.0015415663070940718 88-89 5.4090045862949884E-5 1.6227013758884966E-4 0.0 6.061087134195781 0.0015686113300255467 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2135 0.0 54.553936 2 GTACCCG 225 0.0 52.607338 1 ACCTGGG 2300 0.0 50.84476 3 GTACCTG 2475 0.0 47.442257 1 CCTGGGG 2475 0.0 41.702145 4 CTGGGGG 2200 0.0 40.88804 5 TAGTACT 2760 0.0 35.337452 4 GTACTAG 2105 0.0 34.413445 1 CTGTGCG 2870 0.0 32.993256 9 ACTGTGC 3050 0.0 31.200502 8 GTACTGT 3090 0.0 31.103045 6 GTACACG 245 0.0 30.920229 1 TGGGGGG 5185 0.0 30.680037 6 CTAGTAC 3350 0.0 30.668465 3 GCATCGT 2165 0.0 30.177729 8 TAGGCAT 2630 0.0 29.342506 5 TACTGTG 3340 0.0 28.916721 7 TCTAACG 1075 0.0 27.306879 2 CCTAGTA 3935 0.0 26.470781 2 GGGGGGA 5220 0.0 26.392944 7 >>END_MODULE