FastQCFastQC Report
Sat 18 Jun 2016
SRR1931096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6571252
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA532710.8106674344554128No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC453440.6900359322698323No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG404970.616275254700322No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC364020.5539583628812287No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC334710.509354990494962No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC327950.49906775755974664No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA317030.48244991974132173No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC315600.4802737743127185No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG277780.4227200539562324No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA269220.40969361698501294No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA262630.3996650866531979No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT257450.3917822661495861No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC254120.3867147386829785No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA251270.38237766562597203No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT220060.334882911201701No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA213950.32558483527948706No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC190630.290096925213034No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA189550.2884534027914315No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA184980.2814988681000211No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA181170.27570088622381245No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG159110.2421304189825622No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA147960.22516257175953683No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA147540.22452342415113588No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG145710.22173856671453174No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA142020.21612319844072334No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG141150.21479924982332135No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT140880.21438836921792073No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG130620.19877490621269736No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC128350.19532046556729218No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC125650.19121165951328606No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG122760.18681371525547946No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT119160.1813353071834713No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA118620.18051354597267005No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC118460.18027006116946967No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA116140.1767395315230644No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA115720.17610038391466346No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT113700.17302638827425887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113450.17264594326925828No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT113160.17220462706345763No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT110300.16785233620625112No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG109420.1665131697886491No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA104020.1582955576806368No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA103160.15698682686343485No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG102680.15625637245383375No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG99970.1521323485996276No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT97210.1479322357444213No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT96610.14701916773241994No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC94630.14400604329281544No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT93790.14272774807601352No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC91020.13851241742060721No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT90290.13740151800600556No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA88530.13472318517080153No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA84320.12831649128659195No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA84040.12789039288099133No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC83850.1276012546771909No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT82930.1262012170587888No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG82760.1259425144553884No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC81930.12467943703878652No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG80420.12238154920858309No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC78660.11970321637337908No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA78460.11939886036937862No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA78330.11920102896677834No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA77830.11844013895677717No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT76540.11647704273097426No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA75710.11521396531437235No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA72930.11098341685876605No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT72260.10996382424536451No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT70550.1073615804111606No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC70550.1073615804111606No Hit
GCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAAT70380.10710287780776023No Hit
ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT69920.10640285899855917No Hit
CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG69650.10599197839315855No Hit
ATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTC69360.1055506621873579No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC69310.10547457318635779No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA69090.10513978158195729No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT68070.10358756596155495No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC68020.10351147696055485No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC66390.10103097552795115No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC65740.10004181851494966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG39300.047.5872422
ACCTGGG39700.047.464473
CCTGGGG38950.041.5714074
GTACCTG47050.039.748751
CTGGGGG40100.037.426665
GTACTAG39750.032.0406381
TATTCTC63450.032.0104565
TATTCGC17000.031.471369
TATATCC94150.031.177944
TCTCTGC67800.030.0961178
ATATATC100450.029.4110643
TTCTCTG71150.028.6788647
TCTAACG22850.028.3870562
TGGGGGG128100.028.0882076
CCCATTT106450.027.3536629
CTGTGCG48550.026.818199
CTAACGC24600.026.559513
CTTAATA28900.026.2124822
ACTGTGC51950.026.0641378
AACCGTG8300.025.6681167
Produced by FastQC (version 0.11.5)