##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931096_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6571252 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.505453146523678 33.0 33.0 33.0 27.0 33.0 2 31.451405607333275 33.0 33.0 33.0 27.0 33.0 3 31.459421127054632 33.0 33.0 33.0 27.0 33.0 4 35.1324787118193 37.0 37.0 37.0 33.0 37.0 5 35.13589799934624 37.0 37.0 37.0 33.0 37.0 6 35.224059737779044 37.0 37.0 37.0 33.0 37.0 7 35.18933941355468 37.0 37.0 37.0 33.0 37.0 8 35.198066517613384 37.0 37.0 37.0 33.0 37.0 9 35.19683996291727 37.0 37.0 37.0 33.0 37.0 10-11 35.1239955490978 37.0 37.0 37.0 33.0 37.0 12-13 35.05382642455349 37.0 37.0 37.0 33.0 37.0 14-15 36.81976121140994 40.0 37.0 40.0 33.0 40.0 16-17 36.78646786031034 40.0 37.0 40.0 33.0 40.0 18-19 36.72362907403338 40.0 37.0 40.0 33.0 40.0 20-21 36.6471239422868 40.0 37.0 40.0 33.0 40.0 22-23 36.57983060153529 40.0 37.0 40.0 33.0 40.0 24-25 36.466644560275576 40.0 37.0 40.0 33.0 40.0 26-27 36.28798629241429 40.0 37.0 40.0 33.0 40.0 28-29 36.20858262626361 40.0 37.0 40.0 33.0 40.0 30-31 36.105418191236616 40.0 37.0 40.0 33.0 40.0 32-33 35.967086789549384 40.0 37.0 40.0 33.0 40.0 34-35 35.87699109697817 40.0 37.0 40.0 33.0 40.0 36-37 35.696672871471065 40.0 37.0 40.0 27.0 40.0 38-39 35.53901828753486 37.0 37.0 40.0 27.0 40.0 40-41 35.32460298281058 37.0 37.0 40.0 27.0 40.0 42-43 35.17431023798814 37.0 37.0 40.0 27.0 40.0 44-45 34.93748443979929 37.0 37.0 40.0 27.0 40.0 46-47 34.81897947301367 37.0 37.0 40.0 27.0 40.0 48-49 34.83056227336891 37.0 37.0 40.0 27.0 40.0 50-51 34.016943955276716 37.0 35.0 38.5 24.5 40.0 52-53 34.12988833786925 37.0 33.0 38.5 24.5 40.0 54-55 34.6686399334556 37.0 37.0 40.0 27.0 40.0 56-57 34.63361776416427 37.0 37.0 40.0 27.0 40.0 58-59 34.49653658085248 37.0 33.0 40.0 27.0 40.0 60-61 34.520803341585435 37.0 33.0 40.0 27.0 40.0 62-63 34.382503669011626 37.0 33.0 40.0 27.0 40.0 64-65 34.18686591231017 37.0 33.0 40.0 22.0 40.0 66-67 33.98732311589937 37.0 33.0 40.0 22.0 40.0 68-69 33.792613645010114 37.0 33.0 40.0 22.0 40.0 70-71 33.584967902615816 37.0 33.0 40.0 22.0 40.0 72-73 32.9762559707039 37.0 33.0 40.0 15.0 40.0 74-75 32.77835662062572 37.0 33.0 37.0 15.0 40.0 76-77 32.411593635428986 37.0 33.0 37.0 15.0 40.0 78-79 32.144491719386195 37.0 33.0 37.0 15.0 38.5 80-81 31.84681161215549 37.0 33.0 37.0 15.0 37.0 82-83 31.577113006775573 37.0 33.0 37.0 15.0 37.0 84-85 31.202590465256847 37.0 33.0 37.0 6.0 37.0 86-87 31.03967744655052 37.0 33.0 37.0 4.0 37.0 88-89 30.827991073847116 37.0 33.0 37.0 2.0 37.0 90-91 30.542235482675146 37.0 33.0 37.0 2.0 37.0 92-93 30.246733879632067 33.0 33.0 37.0 2.0 37.0 94-95 30.00385482096867 33.0 33.0 37.0 2.0 37.0 96-97 29.6982502725508 33.0 33.0 37.0 2.0 37.0 98-99 29.3743002094578 33.0 33.0 37.0 2.0 37.0 100-101 28.655071590619258 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1685350741921141 1101 2 0.14738864135867047 1101 3 0.10135927689976398 1101 4 0.16973686452302417 1101 5 0.15546386590895622 1101 6 0.14656677904698512 1101 7 0.23485577637737265 1101 8 0.22919156144794783 1101 9 0.24201113660779328 1101 10-11 0.2326001975527774 1101 12-13 0.2769940547355674 1101 14-15 0.41957938325084143 1101 16-17 0.39700309650562104 1101 18-19 0.4149369169178456 1101 20-21 0.438026785359412 1101 22-23 0.405319106777263 1101 24-25 0.3271175382009375 1101 26-27 0.36699758735473864 1101 28-29 0.3539202461178448 1101 30-31 0.3744799500385909 1101 32-33 0.425855010286206 1101 34-35 0.48859044537067575 1101 36-37 0.4169178474502644 1101 38-39 0.5036151250659984 1101 40-41 0.42215926615006794 1101 42-43 0.363379654692487 1101 44-45 0.31386587419736856 1101 46-47 0.33177209102586147 1101 48-49 0.34683344679148576 1101 50-51 0.41052554666558905 1101 52-53 0.4160013742385331 1101 54-55 0.5609481774113974 1101 56-57 0.5698317652982183 1101 58-59 0.4637364277999012 1101 60-61 0.5186057670991957 1101 62-63 0.5894942906516007 1101 64-65 0.6003924146346478 1101 66-67 0.5962695173042079 1101 68-69 0.5393509153883471 1101 70-71 0.5661829000329064 1101 72-73 0.4036816950759601 1101 74-75 0.47108852017847624 1101 76-77 0.3893204596866653 1101 78-79 0.4012980600245868 1101 80-81 0.5569084176365351 1101 82-83 0.4436367238497745 1101 84-85 0.5602971088466155 1101 86-87 0.5393172685746421 1101 88-89 0.5530334679136217 1101 90-91 0.4200539325729764 1101 92-93 0.4594057019281763 1101 94-95 0.4760736184085417 1101 96-97 0.3870427513782069 1101 98-99 0.5495751223073739 1101 100-101 0.5711657188800032 1102 1 0.07386958565968271 1102 2 0.0974125112653006 1102 3 0.10731192114322852 1102 4 0.13983794874605593 1102 5 0.07953982267162019 1102 6 0.13788276779597197 1102 7 0.1375588707478883 1102 8 0.10422424415817488 1102 9 0.05838300243794947 1102 10-11 0.12625811873444803 1102 12-13 0.1082486179057014 1102 14-15 0.276835667450932 1102 16-17 0.23047911472439608 1102 18-19 0.12822158172320997 1102 20-21 0.1948918433465181 1102 22-23 0.16878332603101143 1102 24-25 0.17105559228338052 1102 26-27 0.16666044511622857 1102 28-29 0.12543094716117054 1102 30-31 0.19419542380615695 1102 32-33 0.24705384293704213 1102 34-35 0.2065293748298842 1102 36-37 0.23436827943184824 1102 38-39 0.21174746450423498 1102 40-41 0.1682114502832448 1102 42-43 0.21144126671918428 1102 44-45 0.24466189842901542 1102 46-47 0.13680603023246363 1102 48-49 0.21416148345509356 1102 50-51 0.12891645355580295 1102 52-53 0.24177251144176637 1102 54-55 0.3327759837173332 1102 56-57 0.2811760419572167 1102 58-59 0.3145784565992287 1102 60-61 0.3350359933384439 1102 62-63 0.34154070852964225 1102 64-65 0.3295602010751466 1102 66-67 0.35428982942871556 1102 68-69 0.3823612680608619 1102 70-71 0.3457641965527145 1102 72-73 0.27882481318538765 1102 74-75 0.31032816481850745 1102 76-77 0.230100560661036 1102 78-79 0.22452035702567485 1102 80-81 0.3594787956786405 1102 82-83 0.3306057109462728 1102 84-85 0.41853894087120125 1102 86-87 0.3854972493741542 1102 88-89 0.3769586010543655 1102 90-91 0.43882157690426027 1102 92-93 0.3440861356596798 1102 94-95 0.3167698648444315 1102 96-97 0.3235712539617168 1102 98-99 0.33246987731319777 1102 100-101 0.2910840560142489 1103 1 -0.0010765507732166668 1103 2 -0.06188524225463965 1103 3 -0.043055836624262156 1103 4 0.06122071376105964 1103 5 -8.315727786936122E-4 1103 6 -0.033873346331652954 1103 7 0.011323226209441373 1103 8 -0.014216767509154238 1103 9 -0.03841864889470514 1103 10-11 -0.003960766436257757 1103 12-13 0.024772481948666325 1103 14-15 0.08164077516125445 1103 16-17 0.10783087777091538 1103 18-19 0.10305994966581267 1103 20-21 0.13120494731448673 1103 22-23 0.073136599016415 1103 24-25 0.0353943542393651 1103 26-27 0.07196759661758989 1103 28-29 0.06145990713071825 1103 30-31 0.022186100045161083 1103 32-33 0.044166494921405786 1103 34-35 -0.004418898004153959 1103 36-37 -0.015219014230837047 1103 38-39 0.03253866355055379 1103 40-41 0.07255670018465565 1103 42-43 0.006859366168036729 1103 44-45 0.07022138722567917 1103 46-47 -0.1770346691466571 1103 48-49 -0.12545636464790988 1103 50-51 -0.005800817651625323 1103 52-53 0.079227559425604 1103 54-55 0.17354586121118842 1103 56-57 0.12456813073322337 1103 58-59 0.06829417838646634 1103 60-61 0.10306786080746377 1103 62-63 0.12191356798599173 1103 64-65 0.0983466933558077 1103 66-67 0.11981145037179886 1103 68-69 0.048663683957570925 1103 70-71 0.13631630935055483 1103 72-73 -0.06605315901570208 1103 74-75 0.0840090278222263 1103 76-77 -0.03553485849216287 1103 78-79 0.015250176998144127 1103 80-81 0.10256729481658766 1103 82-83 0.18085551007968093 1103 84-85 0.19837389528106186 1103 86-87 0.14772234777548832 1103 88-89 0.1350363302359625 1103 90-91 0.10328423898778993 1103 92-93 0.05398892620960183 1103 94-95 0.12483869970680672 1103 96-97 0.1537208979876823 1103 98-99 0.14875843064417893 1103 100-101 0.18286602487464876 1104 1 -0.025721024447967977 1104 2 -4.832124703106899E-4 1104 3 -0.0153976592811631 1104 4 0.015092430878929974 1104 5 0.023506168655430315 1104 6 -0.016815924072624 1104 7 -0.024293614496002647 1104 8 -0.02992184048229518 1104 9 -0.05291957312058315 1104 10-11 -0.004367375198818024 1104 12-13 -0.044635140507644167 1104 14-15 0.029236207216705168 1104 16-17 0.05849621502348157 1104 18-19 0.06329982171642001 1104 20-21 0.10035309743703635 1104 22-23 0.1077574897445217 1104 24-25 0.14938691290900863 1104 26-27 0.17603637551126639 1104 28-29 0.04294248486342411 1104 30-31 -0.006883159247614401 1104 32-33 0.04963028564014138 1104 34-35 0.06885924315507452 1104 36-37 0.10437569526304458 1104 38-39 0.10789303278550477 1104 40-41 0.16786609889854986 1104 42-43 0.13071573221025545 1104 44-45 0.12989829352161308 1104 46-47 -0.021679309153135762 1104 48-49 0.016128406886672053 1104 50-51 -0.054015246526311955 1104 52-53 0.01858042774913571 1104 54-55 0.15334879840225568 1104 56-57 0.12327273198544475 1104 58-59 0.04870414890959296 1104 60-61 0.0990432011469764 1104 62-63 0.11139590128337318 1104 64-65 0.13825096686219496 1104 66-67 0.20101261237972778 1104 68-69 0.17127372427803067 1104 70-71 0.19217892430024364 1104 72-73 0.15616603273193164 1104 74-75 0.16371997116901582 1104 76-77 0.14094576768378175 1104 78-79 0.13637384701582533 1104 80-81 0.16638565457497378 1104 82-83 0.1595406670493098 1104 84-85 0.22834855190418324 1104 86-87 0.11913612202634383 1104 88-89 0.15026260544245318 1104 90-91 0.24949052731899002 1104 92-93 0.16037511418801387 1104 94-95 0.12978027594480324 1104 96-97 0.09479852820889434 1104 98-99 0.18465904257964993 1104 100-101 0.21931101163629663 1105 1 0.12687531596497692 1105 2 0.06636646255209655 1105 3 0.09666726083032273 1105 4 0.13917811417296377 1105 5 0.13263541431877002 1105 6 0.1031723732535994 1105 7 0.0984070348791306 1105 8 0.20757043749318882 1105 9 0.16982871353257423 1105 10-11 0.0721133320661238 1105 12-13 0.13392667049200213 1105 14-15 0.18032272869481147 1105 16-17 0.13166801665518335 1105 18-19 0.13962166554735944 1105 20-21 0.2075368279652139 1105 22-23 0.18569660828811863 1105 24-25 0.14988612548003033 1105 26-27 0.2702302469065714 1105 28-29 0.23491716242359217 1105 30-31 0.15735502134189971 1105 32-33 0.17795039364697374 1105 34-35 0.132049802418166 1105 36-37 0.1143177837045144 1105 38-39 0.12765996540884572 1105 40-41 0.24897458282949003 1105 42-43 0.16173981767602186 1105 44-45 0.27226121127225866 1105 46-47 0.09658944195259522 1105 48-49 0.14513566631480046 1105 50-51 0.1619237009001111 1105 52-53 0.11668436257380677 1105 54-55 0.22030774188429092 1105 56-57 0.2156785015341285 1105 58-59 0.22271090803752713 1105 60-61 0.3019653167351706 1105 62-63 0.265360852718004 1105 64-65 0.2823324157315241 1105 66-67 0.32533992615930174 1105 68-69 0.2931768661631651 1105 70-71 0.2679287945122226 1105 72-73 0.13264293336506228 1105 74-75 0.22512699654365775 1105 76-77 0.17037121280592515 1105 78-79 0.16388423005724206 1105 80-81 0.155044900847372 1105 82-83 0.13506917255841344 1105 84-85 0.2626949931022331 1105 86-87 0.302447993207025 1105 88-89 0.14671801492002245 1105 90-91 0.18700459372984923 1105 92-93 0.20800340130521278 1105 94-95 0.19488901510133871 1105 96-97 0.250725091393182 1105 98-99 0.27661439695168966 1105 100-101 0.2238264020299532 1106 1 0.07364361403567443 1106 2 0.01693926623361719 1106 3 0.03956433288655603 1106 4 0.04583735143252454 1106 5 0.004898660572962399 1106 6 0.0024638746970992997 1106 7 0.019960790787038718 1106 8 0.04107093248645555 1106 9 0.09198944478819726 1106 10-11 0.014716654020922704 1106 12-13 -0.008726240951993702 1106 14-15 0.04613411191696315 1106 16-17 0.06435686695443366 1106 18-19 0.052689127088449084 1106 20-21 0.1060706020138582 1106 22-23 0.10888465343438725 1106 24-25 0.15702196281208103 1106 26-27 0.10019528180855275 1106 28-29 0.18531342752358881 1106 30-31 0.16256083575459002 1106 32-33 0.1655133497575676 1106 34-35 0.05953987099592695 1106 36-37 0.1703513819358875 1106 38-39 0.07870954948827347 1106 40-41 0.17298887902841642 1106 42-43 0.18684526296258497 1106 44-45 0.11386462879952575 1106 46-47 0.2667742574706793 1106 48-49 0.25078399929649464 1106 50-51 0.18843730735432018 1106 52-53 0.22704297572709464 1106 54-55 0.27856078532648354 1106 56-57 0.2143183069269412 1106 58-59 0.3120762166734963 1106 60-61 0.2833755696776379 1106 62-63 0.2865900228784781 1106 64-65 0.3235003473809783 1106 66-67 0.36386879535665884 1106 68-69 0.3809251968006322 1106 70-71 0.3910405620078521 1106 72-73 0.3127885641704182 1106 74-75 0.35080371274572286 1106 76-77 0.25798790589026765 1106 78-79 0.3028072906123285 1106 80-81 0.3240411915535759 1106 82-83 0.35107377446301413 1106 84-85 0.4426750851763934 1106 86-87 0.43436577203068794 1106 88-89 0.36230750771872877 1106 90-91 0.37449019023730656 1106 92-93 0.46432930703468855 1106 94-95 0.5398517147891937 1106 96-97 0.5712134847478332 1106 98-99 0.47803662350172615 1106 100-101 0.5635299109822078 1107 1 0.0198434241657921 1107 2 0.061969453063102975 1107 3 0.04313463128010753 1107 4 0.0855201570392694 1107 5 0.11240348488126983 1107 6 0.04487869695779523 1107 7 0.022414207514202644 1107 8 0.04511652607803285 1107 9 0.051503725361278896 1107 10-11 0.01928842297533606 1107 12-13 0.01811490745055977 1107 14-15 0.05322320804945235 1107 16-17 0.15626288756710238 1107 18-19 0.12168387153972304 1107 20-21 0.16982096655162593 1107 22-23 0.10439230717624781 1107 24-25 0.10700614032180766 1107 26-27 0.11369993237971698 1107 28-29 0.0447988553202876 1107 30-31 0.10077121903114516 1107 32-33 0.14517369363301924 1107 34-35 0.1330322358687539 1107 36-37 0.13596538763198396 1107 38-39 0.11415254819144849 1107 40-41 0.18485667746850254 1107 42-43 0.18788613462668025 1107 44-45 0.16788292083926848 1107 46-47 -0.021761572713664634 1107 48-49 0.02522105991214829 1107 50-51 -9.0826203815908E-4 1107 52-53 0.0830567630107737 1107 54-55 0.16114394647389219 1107 56-57 0.1808103057107857 1107 58-59 0.19589694475624242 1107 60-61 0.16020834146768692 1107 62-63 0.27346169937990084 1107 64-65 0.27027504132437485 1107 66-67 0.25729315454580615 1107 68-69 0.22867747244703196 1107 70-71 0.17265203610549662 1107 72-73 0.2218840141186007 1107 74-75 0.19405526552147734 1107 76-77 0.1992067340332042 1107 78-79 0.29263032691718394 1107 80-81 0.31493860597252166 1107 82-83 0.27683589107786943 1107 84-85 0.43116592984917546 1107 86-87 0.4204106458540835 1107 88-89 0.36716035855917895 1107 90-91 0.40814214509046565 1107 92-93 0.4148657204794617 1107 94-95 0.47542036267984145 1107 96-97 0.4982454337683926 1107 98-99 0.35658163693532785 1107 100-101 0.33111862551345794 1108 1 0.06434091943053133 1108 2 0.08410348949398738 1108 3 0.12352727702305444 1108 4 0.2152858973569991 1108 5 0.1487451630190577 1108 6 0.10173663522176923 1108 7 0.03950057783281835 1108 8 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60-61 -0.42865706766295375 2316 62-63 -0.36517803857604036 2316 64-65 -0.42342860845055696 2316 66-67 -0.415281015148139 2316 68-69 -0.3274016070468875 2316 70-71 -0.27706780391687147 2316 72-73 0.03259964064442045 2316 74-75 -0.20336579831069201 2316 76-77 -0.21920967242713374 2316 78-79 -0.21377054141903784 2316 80-81 -0.21447599304423193 2316 82-83 -0.1763281515391384 2316 84-85 -0.21110775701630402 2316 86-87 -0.26714402443991503 2316 88-89 -0.1816026398308388 2316 90-91 -0.10842699346375184 2316 92-93 -0.11386663807054731 2316 94-95 -0.11215017511862868 2316 96-97 -0.136660234436075 2316 98-99 -0.06536820349773009 2316 100-101 -0.028658806290494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138960.0 3 22739.0 4 17460.0 5 15031.0 6 15338.0 7 16374.0 8 16329.0 9 17058.0 10 17011.0 11 17657.0 12 17838.0 13 18556.0 14 19280.0 15 21749.0 16 25786.0 17 31255.0 18 28105.0 19 22872.0 20 23860.0 21 25776.0 22 28691.0 23 33187.0 24 39387.0 25 46058.0 26 52076.0 27 61562.0 28 74981.0 29 92642.0 30 115413.0 31 145745.0 32 188903.0 33 250798.0 34 348372.0 35 510428.0 36 806269.0 37 1479062.0 38 1767810.0 39 834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.299586454580385 25.2733242279788 13.41527645149877 23.011812865942044 2 13.448704441482281 21.9900481309847 44.38747550420962 20.173771923323404 3 16.95489740140369 32.46008363718263 29.437016453515625 21.148002507898052 4 10.456039303699727 18.94470223649057 44.57559272387276 26.02366573593694 5 11.655398670363248 38.98024658817571 37.127045960824326 12.23730878063672 6 24.021084566533133 43.16185104451937 21.07482714100753 11.742237247939967 7 22.794560306011704 32.61500243789159 28.892987211569427 15.697450044527283 8 21.800518379146013 38.60469816102015 22.00538040543872 17.589403054395113 9 25.231736661445947 15.797217942638634 24.869826937089005 34.101218458826416 10-11 22.14297720045827 28.11743366045778 30.690665345964213 19.048923793119744 12-13 22.826517438741256 25.617053875098716 30.3261459338264 21.230282752333633 14-15 20.771692269669987 26.932947742358554 27.618927539465222 24.67643244850624 16-17 18.92435253693756 29.07621937392957 29.83883314355876 22.160594945574115 18-19 18.379147521673776 30.960113863644406 30.91500669416875 19.74573192051307 20-21 19.302143640207376 28.578298321233152 30.11967125899296 21.999886779566513 22-23 19.688051835479754 30.244137646828946 29.086139140608214 20.98167137708309 24-25 19.877452576769237 29.625313410595123 29.789171074248866 20.708062938386778 26-27 20.20765601440943 28.89260676656442 29.342171020073497 21.55756619895265 28-29 19.54343708017894 30.885841845663503 28.418169018628415 21.152552055529146 30-31 21.092137388735054 29.613633748941602 28.260352821654077 21.033876040669266 32-33 20.93389509335512 29.775003302262643 28.450514452953563 20.84058715142868 34-35 21.51824340323579 28.341783270524402 29.601398637580782 20.538574688659025 36-37 21.464882186834412 29.241604187451646 29.496312118299528 19.797201507414417 38-39 22.375142514698872 28.15116510521891 30.498533612772725 18.975158767309487 40-41 20.72442215031796 28.556249869221737 30.5824740012821 20.1368539791782 42-43 21.520373895263795 28.62172992300402 29.340824244755794 20.517071936976393 44-45 20.595765467851432 30.031873886240128 29.273170390232544 20.099190255675893 46-47 22.208295589458064 28.67759644957757 28.204122455379167 20.909985505585198 48-49 22.00569985492155 27.32969577592414 29.634020227744788 21.030584141409527 50-51 21.68807666952025 28.192360249187555 28.115631436410442 22.003931644881757 52-53 20.182173821515562 28.719147393773742 29.016402317473606 22.082276467237094 54-55 20.844680438614848 29.6592978592327 28.0521285933921 21.44389310876035 56-57 21.637994948339312 28.086128265532896 28.967581539197994 21.308295246929795 58-59 21.334893880741003 27.35052937512975 30.496060215657785 20.818516528471466 60-61 21.07908449038951 30.13709783200142 28.207048545883644 20.57676913172543 62-63 21.815954408520042 28.874077502494444 26.949795183674695 22.36017290531082 64-65 20.02448385717267 29.069151827763047 27.841678178047008 23.06468613701728 66-67 21.305779792605396 26.698240911457237 29.235555095790772 22.760424200146595 68-69 19.746423998094418 28.369374860278906 28.249810597118852 23.634390544507827 70-71 20.41951134894065 28.079147102980073 28.979978730819923 22.52136281725936 72-73 21.515685324259945 27.670277176810437 29.528857123301922 21.28518037562769 74-75 21.538433877266215 29.194758474213174 27.696430976018803 21.570376672501805 76-77 20.447400228872805 29.051911789461528 29.317998252895716 21.182689728769947 78-79 20.532438705972332 28.62562158896908 28.550750259747154 22.291189445311435 80-81 20.673026170610807 29.07811720355148 29.57959355058886 20.669263075248857 82-83 21.966019843507816 27.556437082221358 29.850513563902247 20.62702951036858 84-85 22.990650796699487 27.85604581787997 28.204049324699216 20.94925406072133 86-87 21.53179721013304 29.293351338118622 28.300107862405742 20.874743589342593 88-89 22.288120147851885 27.80590270829616 28.584481685702524 21.321495458149435 90-91 22.046838154121108 27.023904178502683 29.554519244862316 21.374738422513893 92-93 21.760953403682354 28.584569863513487 28.419810178600752 21.234666554203415 94-95 20.55995734918767 27.938607348512956 29.38894386101312 22.112491441286252 96-97 21.39294463132383 27.999315071938017 28.16631010476168 22.441430191976465 98-99 20.262943901693642 29.03286455820745 28.71180199109396 21.992389549004947 100-101 21.239294070721108 27.18043086404709 28.27693628076891 23.303338784462888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 69.0 1 45.5 2 29.0 3 40.0 4 36.5 5 39.0 6 72.0 7 109.5 8 162.5 9 214.5 10 216.5 11 267.0 12 445.0 13 730.5 14 962.5 15 1247.5 16 1688.0 17 2015.0 18 2227.5 19 2352.0 20 2555.0 21 2863.0 22 3302.0 23 3720.0 24 3927.5 25 4269.0 26 4994.5 27 6525.0 28 13050.0 29 34007.0 30 64569.0 31 97749.0 32 143271.5 33 212880.0 34 318864.0 35 395976.0 36 446666.0 37 521525.5 38 510411.5 39 391209.5 40 282520.0 41 210900.5 42 168578.5 43 150011.5 44 136344.0 45 139099.0 46 152966.0 47 173914.5 48 202147.5 49 199541.0 50 174340.0 51 168142.5 52 164627.0 53 158812.5 54 151733.5 55 133690.5 56 118851.5 57 98394.0 58 76148.5 59 70933.5 60 60884.0 61 44795.0 62 33552.5 63 27765.5 64 21931.5 65 13382.5 66 8763.5 67 6629.5 68 5190.0 69 4105.5 70 3276.5 71 2637.0 72 2139.0 73 1707.0 74 1385.5 75 1125.0 76 914.0 77 728.5 78 599.5 79 512.5 80 414.5 81 341.0 82 300.0 83 251.0 84 191.0 85 140.0 86 119.5 87 109.0 88 87.0 89 70.0 90 61.0 91 45.5 92 28.5 93 26.0 94 24.5 95 17.5 96 10.5 97 9.5 98 10.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1088060540061465E-4 2 0.0034240050450051224 3 1.826136024002732E-4 4 9.73939212801457E-4 5 0.0020391852268030505 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.282670030003415E-5 12-13 9.891570130014798E-5 14-15 0.0032413914426048488 16-17 0.004534904459606784 18-19 0.0035685741469053387 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.2065810290033012E-4 42-43 0.0 44-45 6.391476084009562E-4 46-47 0.7574583960560332 48-49 0.5451396476653155 50-51 0.41736338828582437 52-53 0.35001701350062364 54-55 0.34100807578221015 56-57 0.3064712782282585 58-59 0.30636475362685833 60-61 0.2829826036195234 62-63 0.276743305537514 64-65 0.2732279936913088 66-67 0.26138093623559105 68-69 0.24015971385665927 70-71 0.2540992188398801 72-73 0.83554853778245 74-75 0.5498495568272226 76-77 0.6503326915479729 78-79 0.3655239519044468 80-81 0.3164313284591734 82-83 0.29654927249784363 84-85 0.2872435876755297 86-87 0.25974502271408856 88-89 0.26266684035249294 90-91 0.2736693098971094 92-93 0.22882245270764232 94-95 0.1959672220757932 96-97 0.15185842819602718 98-99 0.16034996070763988 100-101 0.15562483374553282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6571252.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.025583638172197 #Duplication Level Percentage of deduplicated Percentage of total 1 77.56847074993432 14.75785427937989 2 10.62805164508393 4.044097709687159 3 4.028339043399212 2.2992450417931884 4 2.016960538121988 1.5349540565173072 5 1.151217022626169 1.0951287874830877 6 0.7635288833531907 0.8715949578237813 7 0.5626630716946632 0.7493495331446385 8 0.38764624807972703 0.5900156891891596 9 0.3042920920983121 0.521040118378575 >10 1.8753963569042822 6.994079329630076 >50 0.26706673949895793 3.608920772780565 >100 0.31661998931146645 13.46758818550587 >500 0.06551771873069204 8.788635235317862 >1k 0.05545664398685497 20.37691686479799 >5k 0.005231758866684431 6.835515003947178 >10k+ 0.003541498309755615 13.465064434623757 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 53271 0.8106674344554128 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 45344 0.6900359322698323 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 40497 0.616275254700322 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 36402 0.5539583628812287 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 33471 0.509354990494962 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 32795 0.49906775755974664 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 31703 0.48244991974132173 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 31560 0.4802737743127185 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 27778 0.4227200539562324 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 26922 0.40969361698501294 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 26263 0.3996650866531979 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 25745 0.3917822661495861 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 25412 0.3867147386829785 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 25127 0.38237766562597203 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 22006 0.334882911201701 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 21395 0.32558483527948706 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 19063 0.290096925213034 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 18955 0.2884534027914315 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 18498 0.2814988681000211 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 18117 0.27570088622381245 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 15911 0.2421304189825622 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 14796 0.22516257175953683 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 14754 0.22452342415113588 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 14571 0.22173856671453174 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 14202 0.21612319844072334 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 14115 0.21479924982332135 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 14088 0.21438836921792073 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 13062 0.19877490621269736 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 12835 0.19532046556729218 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 12565 0.19121165951328606 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 12276 0.18681371525547946 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 11916 0.1813353071834713 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 11862 0.18051354597267005 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 11846 0.18027006116946967 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 11614 0.1767395315230644 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 11572 0.17610038391466346 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 11370 0.17302638827425887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11345 0.17264594326925828 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 11316 0.17220462706345763 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 11030 0.16785233620625112 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 10942 0.1665131697886491 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 10402 0.1582955576806368 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 10316 0.15698682686343485 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 10268 0.15625637245383375 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 9997 0.1521323485996276 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 9721 0.1479322357444213 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 9661 0.14701916773241994 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 9463 0.14400604329281544 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 9379 0.14272774807601352 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 9102 0.13851241742060721 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 9029 0.13740151800600556 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 8853 0.13472318517080153 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 8432 0.12831649128659195 No Hit ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA 8404 0.12789039288099133 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 8385 0.1276012546771909 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 8293 0.1262012170587888 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 8276 0.1259425144553884 No Hit ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC 8193 0.12467943703878652 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 8042 0.12238154920858309 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 7866 0.11970321637337908 No Hit TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA 7846 0.11939886036937862 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 7833 0.11920102896677834 No Hit ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA 7783 0.11844013895677717 No Hit CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT 7654 0.11647704273097426 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 7571 0.11521396531437235 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 7293 0.11098341685876605 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 7226 0.10996382424536451 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 7055 0.1073615804111606 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 7055 0.1073615804111606 No Hit GCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAAT 7038 0.10710287780776023 No Hit ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT 6992 0.10640285899855917 No Hit CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG 6965 0.10599197839315855 No Hit ATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTC 6936 0.1055506621873579 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 6931 0.10547457318635779 No Hit GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA 6909 0.10513978158195729 No Hit GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT 6807 0.10358756596155495 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6802 0.10351147696055485 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 6639 0.10103097552795115 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 6574 0.10004181851494966 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5217800200022767E-5 0.0 10-11 0.0 0.0 0.0 3.0435600400045533E-5 0.0 12-13 0.0 0.0 0.0 3.0435600400045533E-5 0.0 14-15 0.0 0.0 0.0 3.0435600400045533E-5 1.5217800200022767E-5 16-17 0.0 0.0 0.0 6.087120080009107E-5 1.5217800200022767E-5 18-19 0.0 0.0 0.0 7.608900100011383E-5 1.5217800200022767E-5 20-21 0.0 0.0 0.0 8.369790110012522E-5 1.5217800200022767E-5 22-23 0.0 0.0 0.0 1.5978690210023905E-4 2.282670030003415E-5 24-25 0.0 0.0 0.0 3.8044500500056917E-4 6.087120080009107E-5 26-27 0.0 0.0 0.0 8.065434106012066E-4 6.087120080009107E-5 28-29 0.0 0.0 0.0 0.002282670030003415 6.087120080009107E-5 30-31 0.0 0.0 0.0 0.011253563247916836 6.087120080009107E-5 32-33 0.0 0.0 0.0 0.04162829244716228 6.087120080009107E-5 34-35 0.0 0.0 0.0 0.08909261127103328 6.087120080009107E-5 36-37 0.0 0.0 0.0 0.16706861949594992 6.087120080009107E-5 38-39 0.0 0.0 0.0 0.2815977838013213 6.087120080009107E-5 40-41 0.0 0.0 0.0 0.40017488295989867 6.087120080009107E-5 42-43 0.0 0.0 0.0 0.5439450503496137 6.087120080009107E-5 44-45 0.0 0.0 0.0 0.7005514322080479 6.087120080009107E-5 46-47 0.0 0.0 0.0 0.8926305063327353 6.087120080009107E-5 48-49 0.0 0.0 0.0 1.0918467287512335 7.608900100011383E-5 50-51 0.0 0.0 0.0 1.292082543783133 8.369790110012522E-5 52-53 0.0 0.0 0.0 1.4961380266652382 1.2174240160018213E-4 54-55 0.0 0.0 0.0 1.7109829298891597 1.5217800200022767E-4 56-57 0.0 0.0 0.0 1.9757802622696556 1.5217800200022767E-4 58-59 0.0 0.0 0.0 2.2872962412642215 1.5217800200022767E-4 60-61 0.0 0.0 0.0 2.6121810577345075 1.5217800200022767E-4 62-63 0.0 0.0 0.0 2.9235905121276735 1.5217800200022767E-4 64-65 0.0 0.0 0.0 3.25533855648817 1.6739580220025043E-4 66-67 0.0 0.0 0.0 3.6199037869800152 1.7500470230026182E-4 68-69 0.0 0.0 0.0 4.013839371857905 1.826136024002732E-4 70-71 0.0 0.0 0.0 4.409342390156397 1.9022250250028458E-4 72-73 0.0 0.0 0.0 4.875463610283093 1.9783140260029597E-4 74-75 0.0 0.0 0.0 5.295733598407122 1.9783140260029597E-4 76-77 0.0 0.0 0.0 5.780298792376247 2.2065810290033012E-4 78-79 1.5217800200022767E-5 0.0 0.0 6.311247841355042 2.282670030003415E-4 80-81 2.282670030003415E-5 0.0 0.0 6.927751363058364 2.3587590310035288E-4 82-83 3.804450050005692E-5 0.0 0.0 7.587427783929151 2.4348480320036427E-4 84-85 4.56534006000683E-5 0.0 0.0 8.20893035299818 2.4348480320036427E-4 86-87 4.56534006000683E-5 0.0 0.0 8.884189801273791 2.5109370330037565E-4 88-89 4.56534006000683E-5 0.0 0.0 9.602059090109464 2.5870260340038703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3930 0.0 47.587242 2 ACCTGGG 3970 0.0 47.46447 3 CCTGGGG 3895 0.0 41.571407 4 GTACCTG 4705 0.0 39.74875 1 CTGGGGG 4010 0.0 37.42666 5 GTACTAG 3975 0.0 32.040638 1 TATTCTC 6345 0.0 32.010456 5 TATTCGC 1700 0.0 31.47136 9 TATATCC 9415 0.0 31.17794 4 TCTCTGC 6780 0.0 30.096117 8 ATATATC 10045 0.0 29.411064 3 TTCTCTG 7115 0.0 28.678864 7 TCTAACG 2285 0.0 28.387056 2 TGGGGGG 12810 0.0 28.088207 6 CCCATTT 10645 0.0 27.353662 9 CTGTGCG 4855 0.0 26.81819 9 CTAACGC 2460 0.0 26.55951 3 CTTAATA 2890 0.0 26.212482 2 ACTGTGC 5195 0.0 26.064137 8 AACCGTG 830 0.0 25.668116 7 >>END_MODULE