##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931096_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6571252 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.647408439061536 33.0 33.0 33.0 33.0 33.0 2 32.617534527666876 33.0 33.0 33.0 33.0 33.0 3 32.66291035559129 33.0 33.0 33.0 33.0 33.0 4 36.60615267836327 37.0 37.0 37.0 37.0 37.0 5 36.55821873822523 37.0 37.0 37.0 37.0 37.0 6 36.560248336237905 37.0 37.0 37.0 37.0 37.0 7 36.506036444805346 37.0 37.0 37.0 37.0 37.0 8 36.5203970263201 37.0 37.0 37.0 37.0 37.0 9 36.509437470972046 37.0 37.0 37.0 37.0 37.0 10-11 36.49220042086348 37.0 37.0 37.0 37.0 37.0 12-13 36.42863962605604 37.0 37.0 37.0 37.0 37.0 14-15 38.44806126747231 40.0 37.0 40.0 37.0 40.0 16-17 38.45722611155378 40.0 37.0 40.0 37.0 40.0 18-19 38.41079051602343 40.0 37.0 40.0 37.0 40.0 20-21 38.33794328691093 40.0 37.0 40.0 37.0 40.0 22-23 38.23768895181618 40.0 37.0 40.0 37.0 40.0 24-25 38.36960711596512 40.0 37.0 40.0 37.0 40.0 26-27 38.29365979268486 40.0 37.0 40.0 37.0 40.0 28-29 38.250433326860694 40.0 37.0 40.0 37.0 40.0 30-31 38.157740260151336 40.0 37.0 40.0 37.0 40.0 32-33 38.054921117010885 40.0 37.0 40.0 33.0 40.0 34-35 37.99889237241244 40.0 37.0 40.0 33.0 40.0 36-37 37.97208796740712 40.0 37.0 40.0 33.0 40.0 38-39 37.85866445237528 40.0 37.0 40.0 33.0 40.0 40-41 37.713633794595005 40.0 37.0 40.0 33.0 40.0 42-43 37.59000963591109 40.0 37.0 40.0 33.0 40.0 44-45 37.426755890658285 40.0 37.0 40.0 33.0 40.0 46-47 37.255618487922845 40.0 37.0 40.0 33.0 40.0 48-49 37.095704745457944 40.0 37.0 40.0 33.0 40.0 50-51 36.92898118958153 40.0 37.0 40.0 33.0 40.0 52-53 36.74962876176412 37.0 37.0 40.0 33.0 40.0 54-55 36.57096090668871 37.0 37.0 40.0 33.0 40.0 56-57 36.30841831967485 37.0 37.0 40.0 33.0 40.0 58-59 36.08378578389628 37.0 37.0 40.0 33.0 40.0 60-61 35.871339434250885 37.0 37.0 40.0 33.0 40.0 62-63 35.621169147066645 37.0 35.0 40.0 30.0 40.0 64-65 35.31325073212837 37.0 33.0 40.0 27.0 40.0 66-67 35.0204957137544 37.0 33.0 40.0 27.0 40.0 68-69 34.799339760520525 37.0 33.0 40.0 27.0 40.0 70-71 34.5663645984053 37.0 33.0 38.5 27.0 40.0 72-73 34.22426312367871 37.0 33.0 37.0 27.0 40.0 74-75 33.82977307825054 37.0 33.0 37.0 27.0 40.0 76-77 32.184088968129664 33.0 33.0 37.0 24.5 37.0 78-79 32.95560343751845 37.0 33.0 37.0 27.0 37.0 80-81 32.959350820817704 37.0 33.0 37.0 27.0 37.0 82-83 32.72920129984362 37.0 33.0 37.0 27.0 37.0 84-85 32.4124925508868 37.0 33.0 37.0 27.0 37.0 86-87 32.081374979988595 33.0 33.0 37.0 22.0 37.0 88-89 31.744959940662753 33.0 33.0 37.0 22.0 37.0 90-91 31.360543622432985 33.0 33.0 37.0 22.0 37.0 92-93 30.98513517667562 33.0 33.0 37.0 22.0 37.0 94-95 30.568307302778834 33.0 33.0 37.0 15.0 37.0 96-97 30.097424432969547 33.0 33.0 37.0 10.5 37.0 98-99 29.512832410018667 33.0 27.0 37.0 2.0 37.0 100-101 27.758564730130576 33.0 24.5 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.059520715327167295 1101 2 0.151225755223507 1101 3 0.13112283450031725 1101 4 0.15338123340812615 1101 5 0.07700924441323309 1101 6 0.0985022068391288 1101 7 0.07569390113378915 1101 8 0.11676049980216163 1101 9 0.1159089610155064 1101 10-11 0.1245068711133257 1101 12-13 0.16666062717266072 1101 14-15 0.2522444278187379 1101 16-17 0.17468168865174505 1101 18-19 0.1513125977865215 1101 20-21 0.13046327852740802 1101 22-23 0.09854918948155245 1101 24-25 0.18101408277044584 1101 26-27 0.18776081800699274 1101 28-29 0.18794327596782523 1101 30-31 0.15365339555388147 1101 32-33 0.12472736261692319 1101 34-35 0.1573306034510864 1101 36-37 0.05799627403268204 1101 38-39 0.02838773219217927 1101 40-41 0.0037157510806835603 1101 42-43 -0.045710940556041635 1101 44-45 -0.050648936302884806 1101 46-47 -0.18375603810953578 1101 48-49 -0.2316732770255001 1101 50-51 -0.1232731224097634 1101 52-53 -0.2555259803193195 1101 54-55 -0.29477916554517236 1101 56-57 -0.30886204751824664 1101 58-59 -0.37121215652663864 1101 60-61 -0.3975847233945302 1101 62-63 -0.4992024485281732 1101 64-65 -0.38955709859931176 1101 66-67 -0.5354288088405141 1101 68-69 -0.6236410339800003 1101 70-71 -0.6315553254840083 1101 72-73 -0.721459114550072 1101 74-75 -0.7944115009838626 1101 76-77 -0.8913751919085726 1101 78-79 -0.7069941633782761 1101 80-81 -0.8438661431676593 1101 82-83 -0.9329179573207043 1101 84-85 -0.9418191262024038 1101 86-87 -1.038620571580573 1101 88-89 -1.2171453330921729 1101 90-91 -1.2299847728757776 1101 92-93 -1.3383174043056236 1101 94-95 -1.4615787048041078 1101 96-97 -1.5707881339268397 1101 98-99 -1.642083041207428 1101 100-101 -3.092454499367893 1102 1 0.04788552363040566 1102 2 0.1837145867094847 1102 3 0.11631570904388155 1102 4 0.1320622670153213 1102 5 0.1208792661778233 1102 6 0.12486851154202583 1102 7 0.13767700252047632 1102 8 0.10944735605703926 1102 9 0.12597273922080632 1102 10-11 0.12166424631530504 1102 12-13 0.11750370158374324 1102 14-15 0.17660819215499401 1102 16-17 0.15595108129875257 1102 18-19 0.20613444815865023 1102 20-21 0.04949790337336424 1102 22-23 0.15670861202659836 1102 24-25 0.1825488117370071 1102 26-27 0.17851947512898647 1102 28-29 0.20478543799453064 1102 30-31 0.19133830068547297 1102 32-33 0.206065236959887 1102 34-35 0.22519350871155552 1102 36-37 0.19395994654308168 1102 38-39 0.24277744121075528 1102 40-41 0.23914968906544942 1102 42-43 0.21846828916526562 1102 44-45 0.09347735139295565 1102 46-47 0.09769355515042122 1102 48-49 0.13195137020535697 1102 50-51 0.11670166366130275 1102 52-53 0.11611379503393238 1102 54-55 0.06997205492753267 1102 56-57 0.040479660577844356 1102 58-59 0.02198516680125806 1102 60-61 -0.09340695224287998 1102 62-63 -0.18643904468125072 1102 64-65 -0.14118587388189496 1102 66-67 -0.06663563513450299 1102 68-69 -0.1892683273135134 1102 70-71 -0.2536220834478584 1102 72-73 -0.3895471083013149 1102 74-75 -0.34426220172290556 1102 76-77 -0.42949644903701767 1102 78-79 -0.3452360065638871 1102 80-81 -0.4749658118790805 1102 82-83 -0.49961550477958383 1102 84-85 -0.5548429138111644 1102 86-87 -0.6298804497480077 1102 88-89 -0.663689606790566 1102 90-91 -0.690596754164968 1102 92-93 -0.608109234165557 1102 94-95 -0.7441916292585979 1102 96-97 -0.8260613948674198 1102 98-99 -0.793117635859879 1102 100-101 -1.8427857908799297 1103 1 0.0703168257670086 1103 2 0.18525504640518164 1103 3 0.10990581635525132 1103 4 0.111770536653367 1103 5 0.06657106583055139 1103 6 0.11765498705968014 1103 7 0.1597544894104601 1103 8 0.06628854051567856 1103 9 0.0959626830708018 1103 10-11 0.1032357186769346 1103 12-13 0.16312086174175988 1103 14-15 0.20977975695578976 1103 16-17 0.14935796728840955 1103 18-19 0.08808201843521601 1103 20-21 0.09550019913335461 1103 22-23 0.09762482946949547 1103 24-25 0.1665165118485774 1103 26-27 0.20434456936161638 1103 28-29 0.19476456474221493 1103 30-31 0.17630545373851447 1103 32-33 0.2275098651908536 1103 34-35 0.1592446130546037 1103 36-37 0.23117317436431506 1103 38-39 0.18773128256135152 1103 40-41 0.15680319506216023 1103 42-43 0.1678002254342985 1103 44-45 0.12808329748522596 1103 46-47 0.039620281399656676 1103 48-49 0.07161386360009914 1103 50-51 0.05836025072397888 1103 52-53 -0.03684716960213308 1103 54-55 0.11694292440540721 1103 56-57 0.024657339409770884 1103 58-59 0.019844450841091543 1103 60-61 -0.015343709828457008 1103 62-63 -0.08568432888618105 1103 64-65 -0.0887338043646011 1103 66-67 0.010088758586405788 1103 68-69 -0.034814476285589535 1103 70-71 -0.1006041576725707 1103 72-73 -0.018001411268734557 1103 74-75 -0.16616909193088958 1103 76-77 -0.2284930369379552 1103 78-79 -0.10847026280552541 1103 80-81 -0.23370787171408836 1103 82-83 -0.24454732814094626 1103 84-85 -0.2413794096622368 1103 86-87 -0.24419814766218906 1103 88-89 -0.39504590334822964 1103 90-91 -0.26406882123287545 1103 92-93 -0.240153219303604 1103 94-95 -0.3562876497482854 1103 96-97 -0.4043056805154528 1103 98-99 -0.22643562056484612 1103 100-101 -1.0485007107999031 1104 1 0.05777536164280406 1104 2 0.17449937552628825 1104 3 0.08213962669906039 1104 4 0.13165843939827226 1104 5 0.07042629441167492 1104 6 0.042567575086359 1104 7 0.1215444750418726 1104 8 0.09888015830561159 1104 9 0.06939330703587387 1104 10-11 0.08745468582507954 1104 12-13 0.10950338938898341 1104 14-15 0.18016836986487306 1104 16-17 0.15286787673370128 1104 18-19 0.12649098518250668 1104 20-21 0.1314889358924205 1104 22-23 0.1494918411556867 1104 24-25 0.16103265284498747 1104 26-27 0.19503440903436342 1104 28-29 0.17965500585395944 1104 30-31 0.13456869012834005 1104 32-33 0.16627318567634575 1104 34-35 0.12562902200667736 1104 36-37 0.14544115151839065 1104 38-39 0.1599949499673201 1104 40-41 0.1514006164864341 1104 42-43 0.1572157419325535 1104 44-45 0.17098745672894466 1104 46-47 0.13041986902094038 1104 48-49 0.13410807814860704 1104 50-51 0.1704826363714318 1104 52-53 0.16449305089795274 1104 54-55 0.12967275146586843 1104 56-57 0.15044359259848505 1104 58-59 0.14971835795897448 1104 60-61 0.11373539506301711 1104 62-63 0.03552503813170915 1104 64-65 0.05657158069232082 1104 66-67 0.09395006602405687 1104 68-69 0.05099660578806464 1104 70-71 0.024886546759866235 1104 72-73 0.010014999164418725 1104 74-75 -0.11875277125488282 1104 76-77 -0.05084684890995561 1104 78-79 0.01648655293156054 1104 80-81 -0.06176298785082679 1104 82-83 -0.04108668801379167 1104 84-85 -0.04128920827479732 1104 86-87 -0.04403963145693268 1104 88-89 -0.16775617661576803 1104 90-91 -0.03424971577112146 1104 92-93 0.09097384243501239 1104 94-95 0.06679634818878455 1104 96-97 -0.018552411632054344 1104 98-99 0.0295762383709679 1104 100-101 -0.5698572400744943 1105 1 0.08351450077845612 1105 2 0.1552491297266343 1105 3 0.10649257179944982 1105 4 0.12922625545022015 1105 5 0.08734318367566374 1105 6 0.09861584172750071 1105 7 0.1415065972427314 1105 8 0.10767079500647014 1105 9 0.0949194923122576 1105 10-11 0.07256245338416534 1105 12-13 0.10706964653456907 1105 14-15 0.10957673952434988 1105 16-17 0.1707590462179951 1105 18-19 0.12872141764487566 1105 20-21 0.03365722962227835 1105 22-23 0.06691871032067098 1105 24-25 0.11185796687399119 1105 26-27 0.1617680361671674 1105 28-29 0.15966877498500764 1105 30-31 0.13973265807432966 1105 32-33 0.1542126885084798 1105 34-35 0.11864736924690789 1105 36-37 0.11986551628218223 1105 38-39 0.14950829565589885 1105 40-41 0.15419171143325627 1105 42-43 0.1639951719093773 1105 44-45 0.11623065337156646 1105 46-47 0.012859223938242792 1105 48-49 -0.026139265909996823 1105 50-51 0.0599844896573174 1105 52-53 -0.012380609530190156 1105 54-55 0.01712344652057851 1105 56-57 0.10905586140903978 1105 58-59 0.08156822875101 1105 60-61 0.07597273746846156 1105 62-63 -0.049961144882445296 1105 64-65 0.05286841676498 1105 66-67 0.1182384796519429 1105 68-69 0.06657801985389256 1105 70-71 -0.028748061541342906 1105 72-73 -0.07761199732333068 1105 74-75 0.04762589652549565 1105 76-77 -0.0070234896770173805 1105 78-79 0.1843525623923128 1105 80-81 0.07798539016147998 1105 82-83 0.15449580641040228 1105 84-85 0.19568261249882113 1105 86-87 0.19819262818870698 1105 88-89 0.1743545970948368 1105 90-91 0.14685967450072468 1105 92-93 0.23727430387129544 1105 94-95 0.1467956883726096 1105 96-97 0.23038669821066549 1105 98-99 0.10793999575583513 1105 100-101 -0.7823656871717368 1106 1 0.08464189527456512 1106 2 0.19632653801763666 1106 3 0.10674142087248839 1106 4 0.12033549117701625 1106 5 0.10491012497794117 1106 6 0.10871154042537512 1106 7 0.10118114593329608 1106 8 0.13481950757316952 1106 9 0.10948040894241018 1106 10-11 0.1087773868531059 1106 12-13 0.10350569502767826 1106 14-15 0.23523163635116617 1106 16-17 0.20780185366832882 1106 18-19 0.18952949189567647 1106 20-21 0.17344837672634128 1106 22-23 0.18914309644787153 1106 24-25 0.19848268984906525 1106 26-27 0.24483998781109761 1106 28-29 0.2814448721299101 1106 30-31 0.28744856620213 1106 32-33 0.23718131368948292 1106 34-35 0.2007500717839079 1106 36-37 0.19986111634204917 1106 38-39 0.31455401716493014 1106 40-41 0.25034068186530334 1106 42-43 0.3375625315666113 1106 44-45 0.3920495561635491 1106 46-47 0.3965374296282249 1106 48-49 0.41551049524898787 1106 50-51 0.3459995324034537 1106 52-53 0.38518986615022754 1106 54-55 0.3975168444464785 1106 56-57 0.4718130730732142 1106 58-59 0.47197275275502903 1106 60-61 0.37907920339687706 1106 62-63 0.39399533898834704 1106 64-65 0.5789961268305603 1106 66-67 0.6527536657567907 1106 68-69 0.5761695286907411 1106 70-71 0.6463279005398235 1106 72-73 0.6149827591742394 1106 74-75 0.5274316139231132 1106 76-77 0.47881952262297744 1106 78-79 0.774913969507594 1106 80-81 0.682994830196975 1106 82-83 0.6891236958997737 1106 84-85 0.8114437750928332 1106 86-87 0.8648322460430933 1106 88-89 0.8325062976536621 1106 90-91 0.808319805453042 1106 92-93 0.9322554869142117 1106 94-95 1.0749652231150044 1106 96-97 1.1736554209477141 1106 98-99 1.2562024702172465 1106 100-101 1.0702561542032107 1107 1 0.030749252422296536 1107 2 0.11871567200000754 1107 3 0.045392972407270804 1107 4 0.0800315752657923 1107 5 0.0757098743000526 1107 6 0.04706649782875161 1107 7 0.05988630778806936 1107 8 0.0385312776444664 1107 9 0.06486222734986313 1107 10-11 0.07803956241449583 1107 12-13 0.08511809986005403 1107 14-15 0.09650376456917797 1107 16-17 0.07412383824295432 1107 18-19 0.038063894953445754 1107 20-21 0.05177901092898196 1107 22-23 0.10021790890483118 1107 24-25 0.10852500725442837 1107 26-27 0.1642672136631873 1107 28-29 0.14465168759004143 1107 30-31 0.14326658894928812 1107 32-33 0.10374497904692248 1107 34-35 0.11114079422019785 1107 36-37 0.14765000088750213 1107 38-39 0.20747453862457377 1107 40-41 0.2187605425032828 1107 42-43 0.17063960260649225 1107 44-45 0.21183294316924872 1107 46-47 0.2729792928350392 1107 48-49 0.2738877282367085 1107 50-51 0.3184671583473744 1107 52-53 0.37204727138591664 1107 54-55 0.3140119456793897 1107 56-57 0.33457841003021116 1107 58-59 0.38029383141451945 1107 60-61 0.37598885387496495 1107 62-63 0.3497091194466364 1107 64-65 0.5475528054726837 1107 66-67 0.5213581221146413 1107 68-69 0.5370969748167127 1107 70-71 0.43249625183633356 1107 72-73 0.5410765275950382 1107 74-75 0.5153078634344794 1107 76-77 0.4421066915140912 1107 78-79 0.6578926929118509 1107 80-81 0.585256825816316 1107 82-83 0.6547913739648337 1107 84-85 0.7322270814348641 1107 86-87 0.7615312402291039 1107 88-89 0.6977970281563195 1107 90-91 0.7390191595231421 1107 92-93 0.9740119694815554 1107 94-95 1.0426153661379054 1107 96-97 1.0855620000012216 1107 98-99 1.236517120602258 1107 100-101 1.9357373750789684 1108 1 0.06724321527876498 1108 2 0.09723444432638928 1108 3 0.07413223409512426 1108 4 0.08088295135231505 1108 5 0.03934729433813544 1108 6 0.019918263087326693 1108 7 0.09016483952449761 1108 8 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-0.24100213213798583 2316 62-63 -0.3728557786466524 2316 64-65 -0.2871577650232098 2316 66-67 -0.12652000881363534 2316 68-69 -0.12425216960329521 2316 70-71 -0.18953825677090208 2316 72-73 -0.16094636143572671 2316 74-75 -0.10889171948868892 2316 76-77 -0.09358625381415919 2316 78-79 -0.05273899140404836 2316 80-81 0.013081355692229124 2316 82-83 0.03611598494133261 2316 84-85 0.05252595469685417 2316 86-87 0.05521120282742231 2316 88-89 0.039387478946938614 2316 90-91 0.17212510010940107 2316 92-93 0.23429122926350843 2316 94-95 0.3481728092005305 2316 96-97 0.42754071721823195 2316 98-99 0.6336362870127665 2316 100-101 1.0485896388636817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 32.0 5 60.0 6 119.0 7 313.0 8 881.0 9 1769.0 10 3016.0 11 4355.0 12 6200.0 13 8264.0 14 10271.0 15 13185.0 16 16461.0 17 20255.0 18 20903.0 19 19710.0 20 20107.0 21 21931.0 22 24801.0 23 28501.0 24 33835.0 25 40714.0 26 49593.0 27 62208.0 28 77725.0 29 97858.0 30 124136.0 31 160057.0 32 210810.0 33 288201.0 34 415004.0 35 641387.0 36 1058272.0 37 1835142.0 38 1255134.0 39 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.15641099177291 26.521521465874333 14.42723881989361 23.89482872245915 2 12.264401606635941 22.770278269904978 44.16460744278939 20.800712680669694 3 15.836784223158693 32.78041992606584 30.07429938769659 21.30849646307888 4 10.034937025699213 19.594469973149714 43.77795890341749 26.59263409773358 5 11.22359939932299 39.332474237786045 37.35848206703989 12.085444295851081 6 24.074666441037415 43.65347729778131 20.730204837677814 11.541651423503465 7 22.576976198751776 32.77297842176803 28.93299480829528 15.717050571184913 8 21.879331366381933 38.927924237268634 21.728005561192905 17.464738835156528 9 24.944059366464717 15.71470702995411 24.7995663535655 34.54166725001567 10-11 21.963759721891655 28.446831745305158 30.652971458102655 18.936437074700528 12-13 22.543451384911126 25.735955644373398 30.528375718964973 21.192217251750503 14-15 20.568402874527276 27.045052552510278 27.591076078978737 24.795468493983705 16-17 18.790171188078013 29.147398395313406 29.85216515817686 22.210265258431725 18-19 18.328980535216118 30.973899646520938 30.956985061598612 19.740134756664332 20-21 19.1166614824694 28.71683356535406 30.236422222127533 21.93008273004901 22-23 19.570380195433078 30.22147834233111 29.175117618377744 21.033023843858064 24-25 19.657821675382408 29.697278387741026 29.96095721180682 20.68394272506974 26-27 20.036174232855476 29.022467864571315 29.456981713682566 21.484376188890643 28-29 19.36019193907036 31.04270693012534 28.46846384828949 21.12863728251481 30-31 21.051658040202994 29.71156029322875 28.266082323429387 20.97069934313887 32-33 20.961058866712158 29.79712998375348 28.579987497055352 20.66182365247901 34-35 21.399643477376916 28.42848668716403 29.677411549579897 20.494458285879162 36-37 21.34399198204543 29.301965591945034 29.63210815838443 19.721934267625105 38-39 22.059023039384833 28.17803621001948 30.785204787979755 18.977735962615927 40-41 20.448874560685578 28.728652142113404 30.775765275297616 20.046708021903402 42-43 21.30954786962048 28.63593100920916 29.66764715908814 20.386873962082216 44-45 20.346054298328536 30.13231725095918 29.480599739593004 20.04102871111928 46-47 22.03948349568697 28.720873891307164 28.34983348683021 20.889809126175653 48-49 21.82129607584927 27.381195050830183 29.804282857574826 20.993226015745726 50-51 21.346548572479033 28.345907294378602 28.195022805395382 22.112521327746983 52-53 19.94438807094904 28.653276422818664 29.174257812666443 22.228077693565854 54-55 20.54823799178604 29.729479252964275 28.106554123932547 21.61572863131714 56-57 21.580202676750186 28.107056311339147 29.0768486735861 21.235892338324568 58-59 21.16187295815166 27.49374852767783 30.55919937327012 20.785179140900397 60-61 20.974062476983075 30.353964510872512 28.19372929237838 20.478243719766034 62-63 21.702553790358365 28.93396874750809 26.949217592020513 22.414259870113032 64-65 19.822196744242955 29.201984644630883 27.84200027635525 23.133818334770908 66-67 21.14957467053564 26.773937170930363 29.12052161877438 22.955966539759622 68-69 19.54499780275806 28.572812412609693 28.168025278745347 23.714164505886895 70-71 20.165590334060678 28.223022842326156 28.954614001355772 22.65677282225739 72-73 21.21645920746914 27.917457738647066 29.495421876987827 21.37066117689597 74-75 21.139430353303485 29.37867895271348 27.740246012610847 21.741644681372193 76-77 20.027022753624223 29.156686773307904 29.38324601449766 21.43304445857021 78-79 20.091982665419962 28.750910071539536 28.692394602149594 22.46471266089091 80-81 20.13501791209226 29.22131858565998 29.731858870366366 20.911804631881395 82-83 21.38024097764368 27.684396687679758 29.944986922048038 20.99037541262852 84-85 22.494574542224978 27.95332200241223 28.187255278013 21.364848177349792 86-87 20.97785101288557 29.36184967614871 28.248153388728376 21.41214592223734 88-89 21.502074490523267 28.017750650865313 28.547604018229706 21.93257084038171 90-91 21.217375504298953 27.160619796747987 29.676746482519086 21.94525821643398 92-93 20.889095413467693 28.479751253369923 28.547379852140814 22.083773481021574 94-95 19.405922000478164 28.00321230183158 29.598002675091983 22.99286302259827 96-97 20.321401398607573 28.027127535682922 28.21052328814842 23.440947777561085 98-99 19.053431522638302 29.087934841031814 28.88346086864421 22.97517276768567 100-101 18.428196200412707 28.049074451701657 28.703273659157176 24.81945568872846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 72.0 1 47.0 2 26.0 3 32.5 4 29.0 5 30.0 6 61.5 7 124.5 8 178.5 9 210.0 10 236.0 11 260.0 12 310.5 13 406.5 14 508.5 15 634.0 16 801.0 17 948.5 18 1112.0 19 1316.0 20 1499.0 21 1692.0 22 1878.0 23 2040.5 24 2321.5 25 2685.5 26 3343.5 27 4964.5 28 11653.5 29 33099.5 30 64218.5 31 98050.5 32 145919.5 33 217029.5 34 325458.5 35 405036.0 36 451532.0 37 520053.0 38 508679.0 39 388929.5 40 276105.0 41 205286.0 42 171942.0 43 156439.5 44 141471.0 45 149981.5 46 161720.0 47 179959.0 48 210360.5 49 204591.0 50 178003.5 51 172819.0 52 168654.0 53 160406.5 54 153879.5 55 136011.5 56 116726.5 57 94704.5 58 73937.0 59 70337.5 60 59064.5 61 40196.0 62 27914.5 63 22339.0 64 16589.5 65 8259.0 66 4461.0 67 2992.0 68 2231.0 69 1640.5 70 1208.0 71 907.5 72 686.0 73 516.5 74 375.0 75 272.0 76 204.0 77 142.5 78 112.0 79 94.5 80 67.5 81 59.5 82 48.0 83 35.5 84 28.5 85 19.0 86 15.0 87 12.5 88 14.5 89 12.0 90 5.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08377399010112532 2 9.13068012001366E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.282670030003415E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.848010090010245E-5 40-41 0.003317480443604963 42-43 0.006147991280809197 44-45 0.0 46-47 0.0 48-49 1.2935130170019352E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002579417133903859 68-69 0.007829558202911714 70-71 0.001453299919102174 72-73 0.0 74-75 5.3262300700079677E-5 76-77 0.0018717894246028004 78-79 0.00517405206800774 80-81 0.0041392416544061925 82-83 0.004093588253806124 84-85 0.002244625529503358 86-87 3.9566280520059193E-4 88-89 0.0 90-91 1.5217800200022765E-5 92-93 0.0010272015135015366 94-95 0.004002281452605987 96-97 0.0032946537433049287 98-99 0.0 100-101 1.826136024002732E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6571252.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.367806054061209 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9656617077973 10.052530578616885 2 14.188083806605853 4.077032728241588 3 5.469110102390452 2.3573733971835855 4 2.8038544655591515 1.611409486598693 5 1.5655565811021868 1.1246806661967683 6 0.970979635565117 0.837050825174558 7 0.7240999092512398 0.7282608941919594 8 0.5186200980521352 0.5961146387641029 9 0.3989362759753477 0.515865513702891 >10 2.421331998613646 6.891208343904748 >50 0.35046264330920085 3.5774480466172327 >100 0.4358534289714712 13.972498021268784 >500 0.09255178215494266 9.446256680540909 >1k 0.08327527864401967 23.269802947547085 >5k 0.0066108415824958 6.600281562848704 >10k+ 0.005011444425440365 14.342185668601523 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 56942 0.8665319789896964 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 48771 0.7421873335553103 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 40217 0.6120142706443156 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 39299 0.5980443300606947 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 37426 0.5695413902860521 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 32190 0.4898609884387328 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 31615 0.4811107533237198 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 29497 0.44887945250007155 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 29295 0.44580545685966694 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 27934 0.42509403078743596 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 27204 0.4139850366414193 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 26948 0.4100892797902135 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 25687 0.39089963373798475 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 23491 0.35748134449873475 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 21971 0.3343502881947002 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 20475 0.31158445909546617 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 20083 0.3056190814170572 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 19417 0.29548402648384203 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 19220 0.29248611984443756 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 16083 0.24474788061696612 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 15408 0.2344758654819508 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 15389 0.23418672727815038 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 15385 0.23412585607735023 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 15276 0.23246711585554777 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 15056 0.22911919981154277 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 14932 0.22723219258673996 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 14244 0.21676234604912428 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 13942 0.2121665703887174 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 13850 0.21076653277031532 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 13478 0.20510551109590683 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 13051 0.1986075104104971 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 13041 0.1984553324084969 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 12894 0.19621831577909354 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12857 0.19565525717169271 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 12332 0.18766591206668073 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 12272 0.18675284405467937 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 11820 0.1798743983642691 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 11740 0.17865697434826727 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 11493 0.17489817769886165 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 11331 0.17243289406645795 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 10751 0.16360656995044476 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 10464 0.15923906129303822 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 10458 0.15914775449183807 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 10385 0.15803685507723644 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 10309 0.1568803022620347 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 10214 0.15543461124303254 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 10044 0.15284758520902866 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 9613 0.14628871332281884 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 9503 0.14461475530081633 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 9318 0.14179946226381213 No Hit ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC 9231 0.14047551364641014 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 9198 0.1399733262398094 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 9137 0.139045040427608 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 9096 0.13842111061940707 No Hit TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA 8912 0.1356210353826029 No Hit ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA 8848 0.13464709616980144 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 8846 0.1346166605694014 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 8837 0.13447970036760118 No Hit CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT 8739 0.13298835594799896 No Hit ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT 8553 0.1301578451107947 No Hit ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA 8232 0.12527293124658742 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 8154 0.12408594283098563 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 8149 0.12400985382998553 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 8034 0.1222598068069829 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 7994 0.12165109479898199 No Hit CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG 7545 0.11481830250917177 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 7416 0.11285520628336883 No Hit ATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTC 7379 0.112292147675968 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 7364 0.11206388067296766 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 7348 0.11182039586976728 No Hit GCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAAT 7312 0.11127255506256646 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6952 0.10579414699055827 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 6933 0.10550500878675784 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 6885 0.10477455437715674 No Hit GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA 6784 0.10323755655695444 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 6744 0.10262884454895355 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 6722 0.10229405294455303 No Hit CCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATA 6703 0.1020049147407526 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5217800200022767E-5 0.0 10-11 0.0 0.0 0.0 3.0435600400045533E-5 0.0 12-13 0.0 0.0 0.0 3.0435600400045533E-5 0.0 14-15 0.0 0.0 0.0 3.0435600400045533E-5 0.0 16-17 0.0 0.0 0.0 6.087120080009107E-5 0.0 18-19 0.0 0.0 0.0 6.087120080009107E-5 0.0 20-21 0.0 0.0 0.0 6.848010090010245E-5 0.0 22-23 0.0 0.0 0.0 1.369602018002049E-4 7.608900100011383E-6 24-25 0.0 0.0 0.0 3.5000940460052363E-4 1.5217800200022767E-5 26-27 0.0 0.0 0.0 7.761078102011611E-4 1.5217800200022767E-5 28-29 0.0 0.0 0.0 0.0022826700300034148 1.5217800200022767E-5 30-31 1.5217800200022767E-5 0.0 0.0 0.011268781048116858 1.5217800200022767E-5 32-33 1.5217800200022767E-5 0.0 0.0 0.041635901347262284 1.5217800200022767E-5 34-35 1.5217800200022767E-5 0.0 0.0 0.08919913587243344 1.5217800200022767E-5 36-37 1.5217800200022767E-5 0.0 0.0 0.1675099357017506 1.5217800200022767E-5 38-39 3.0435600400045533E-5 0.0 0.0 0.2824728073128226 1.5217800200022767E-5 40-41 3.0435600400045533E-5 0.0 0.0 0.40184123208180117 3.0435600400045533E-5 42-43 3.0435600400045533E-5 0.0 0.0 0.5465929475844178 3.804450050005692E-5 44-45 3.0435600400045533E-5 0.0 0.0 0.7038384770512529 4.56534006000683E-5 46-47 3.0435600400045533E-5 0.0 0.0 0.8962066893797407 4.56534006000683E-5 48-49 3.0435600400045533E-5 0.0 0.0 1.0951033379940383 4.56534006000683E-5 50-51 3.0435600400045533E-5 0.0 0.0 1.294920663520437 4.56534006000683E-5 52-53 3.0435600400045533E-5 0.0 0.0 1.4983598254944415 8.369790110012522E-5 54-55 3.0435600400045533E-5 0.0 0.0 1.712497101009062 1.2174240160018213E-4 56-57 3.804450050005692E-5 0.0 0.0 1.975886786871056 1.2935130170019352E-4 58-59 4.56534006000683E-5 0.0 0.0 2.2858048968446196 1.369602018002049E-4 60-61 4.56534006000683E-5 0.0 0.0 2.6092440222959032 1.369602018002049E-4 62-63 4.56534006000683E-5 0.0 0.0 2.9207904368908695 1.5217800200022767E-4 64-65 4.56534006000683E-5 0.0 0.0 3.2518993336429647 1.5217800200022767E-4 66-67 4.56534006000683E-5 0.0 0.0 3.61615259923071 1.5217800200022767E-4 68-69 4.56534006000683E-5 0.0 0.0 4.009494689900798 1.5217800200022767E-4 70-71 4.56534006000683E-5 0.0 0.0 4.404175946988488 1.5217800200022767E-4 72-73 4.56534006000683E-5 0.0 0.0 4.869505841504784 1.6739580220025043E-4 74-75 4.56534006000683E-5 0.0 0.0 5.288459489911512 1.6739580220025043E-4 76-77 4.56534006000683E-5 0.0 0.0 5.7715105127607345 1.6739580220025043E-4 78-79 4.56534006000683E-5 0.0 0.0 6.2975442122749214 1.6739580220025043E-4 80-81 4.56534006000683E-5 0.0 0.0 6.908987815411736 1.6739580220025043E-4 82-83 4.56534006000683E-5 0.0 0.0 7.564829350632117 1.6739580220025043E-4 84-85 4.56534006000683E-5 1.5217800200022767E-5 0.0 8.181819841941842 1.6739580220025043E-4 86-87 4.56534006000683E-5 1.5217800200022767E-5 0.0 8.84978996392164 1.6739580220025043E-4 88-89 4.56534006000683E-5 1.5217800200022767E-5 0.0 9.558657923939 1.6739580220025043E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3705 0.0 48.715782 3 TACCTGG 3885 0.0 46.94772 2 CCTGGGG 3660 0.0 42.436638 4 GTACCCG 395 0.0 42.125523 1 CTGGGGG 3455 0.0 42.067596 5 GTACCTG 4730 0.0 38.99823 1 TATTCTC 5760 0.0 34.386494 5 TCTCTGC 6100 0.0 32.547733 8 GTACTAG 4120 0.0 30.69437 1 TATTCGC 1750 0.0 30.670065 9 TTCTCTG 6500 0.0 30.471724 7 TGGGGGG 11985 0.0 29.96112 6 TATATCC 9475 0.0 29.92743 4 CTGTGCG 5365 0.0 29.569983 9 GTACACG 460 0.0 28.938402 1 TAGTACT 5630 0.0 28.009413 4 ATATATC 10330 0.0 27.496357 3 ACTGTGC 5910 0.0 27.164614 8 CTTAATA 2765 0.0 26.28274 2 TCTAACG 2085 0.0 26.197878 2 >>END_MODULE