##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931095_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4489313 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.484393491832716 33.0 33.0 33.0 27.0 33.0 2 31.425466881012753 33.0 33.0 33.0 27.0 33.0 3 31.44147512102631 33.0 33.0 33.0 27.0 33.0 4 35.11822432519185 37.0 37.0 37.0 33.0 37.0 5 35.11539694380855 37.0 37.0 37.0 33.0 37.0 6 35.2048173072361 37.0 37.0 37.0 33.0 37.0 7 35.17233371787621 37.0 37.0 37.0 33.0 37.0 8 35.1721499481101 37.0 37.0 37.0 33.0 37.0 9 35.18174584841823 37.0 37.0 37.0 33.0 37.0 10-11 35.10437688350088 37.0 37.0 37.0 33.0 37.0 12-13 35.042175829575704 37.0 37.0 37.0 33.0 37.0 14-15 36.806580093657985 40.0 37.0 40.0 33.0 40.0 16-17 36.77284831777156 40.0 37.0 40.0 33.0 40.0 18-19 36.72153968769832 40.0 37.0 40.0 33.0 40.0 20-21 36.64422106455932 40.0 37.0 40.0 33.0 40.0 22-23 36.58254570354083 40.0 37.0 40.0 33.0 40.0 24-25 36.48338632213882 40.0 37.0 40.0 33.0 40.0 26-27 36.33486381992077 40.0 37.0 40.0 33.0 40.0 28-29 36.251060685677295 40.0 37.0 40.0 33.0 40.0 30-31 36.14530786781853 40.0 37.0 40.0 33.0 40.0 32-33 36.01723415141693 40.0 37.0 40.0 33.0 40.0 34-35 35.93705072468772 40.0 37.0 40.0 33.0 40.0 36-37 35.76742722104696 40.0 37.0 40.0 30.0 40.0 38-39 35.629221442122656 38.5 37.0 40.0 27.0 40.0 40-41 35.445288287985264 37.0 37.0 40.0 27.0 40.0 42-43 35.3198288914139 37.0 37.0 40.0 27.0 40.0 44-45 35.125374639727724 37.0 37.0 40.0 27.0 40.0 46-47 35.00846276479274 37.0 37.0 40.0 27.0 40.0 48-49 35.04931478379876 37.0 37.0 40.0 27.0 40.0 50-51 34.28020746158711 37.0 35.0 38.5 24.5 40.0 52-53 34.40307113805609 37.0 37.0 38.5 27.0 40.0 54-55 34.95722418998186 37.0 37.0 40.0 27.0 40.0 56-57 34.95682067612573 37.0 37.0 40.0 27.0 40.0 58-59 34.85155401283002 37.0 37.0 40.0 27.0 40.0 60-61 34.89263045370194 37.0 37.0 40.0 27.0 40.0 62-63 34.75354770317864 37.0 37.0 40.0 27.0 40.0 64-65 34.573187256045635 37.0 37.0 40.0 27.0 40.0 66-67 34.364552772328416 37.0 33.0 40.0 27.0 40.0 68-69 34.16475427309257 37.0 33.0 40.0 22.0 40.0 70-71 33.93827641333986 37.0 33.0 40.0 22.0 40.0 72-73 33.3285461494888 37.0 33.0 40.0 22.0 40.0 74-75 33.135258334627146 37.0 33.0 37.0 22.0 40.0 76-77 32.75531423182122 37.0 33.0 37.0 15.0 40.0 78-79 32.482948393217406 37.0 33.0 37.0 15.0 38.5 80-81 32.20284551333356 37.0 33.0 37.0 15.0 37.0 82-83 31.92352281963855 37.0 33.0 37.0 15.0 37.0 84-85 31.53462311493986 37.0 33.0 37.0 15.0 37.0 86-87 31.35557467256126 37.0 33.0 37.0 10.5 37.0 88-89 31.14551937011298 37.0 33.0 37.0 6.0 37.0 90-91 30.864434714175644 37.0 33.0 37.0 2.0 37.0 92-93 30.597938370525736 35.0 33.0 37.0 2.0 37.0 94-95 30.357274375834344 33.0 33.0 37.0 2.0 37.0 96-97 30.079885496956884 33.0 33.0 37.0 2.0 37.0 98-99 29.762553201347288 33.0 33.0 37.0 2.0 37.0 100-101 29.072008233776526 33.0 30.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03174504425914648 1101 2 0.039391940613601406 1101 3 0.03885781821841405 1101 4 0.07622108677810502 1101 5 -0.050365036203658065 1101 6 -0.0035766182995260465 1101 7 0.04789543423571985 1101 8 -0.061970186153956774 1101 9 0.005430091799468073 1101 10-11 -6.138174533063534E-4 1101 12-13 0.017743892510672765 1101 14-15 0.15996771221642092 1101 16-17 0.11128082928635763 1101 18-19 0.11717575975594485 1101 20-21 0.1455564110099985 1101 22-23 0.1313434617398741 1101 24-25 0.10904446776314813 1101 26-27 0.14157480545748058 1101 28-29 0.17616017245068605 1101 30-31 0.17077340022782295 1101 32-33 0.17476852771207518 1101 34-35 0.18530508387183886 1101 36-37 0.254285531720285 1101 38-39 0.20738413033624425 1101 40-41 0.1954285779363545 1101 42-43 0.1930969229159345 1101 44-45 0.059743714805797765 1101 46-47 0.08835732550748077 1101 48-49 0.10122062006928445 1101 50-51 0.07433980773291893 1101 52-53 0.18489611865414446 1101 54-55 0.23658689301326774 1101 56-57 0.2812601087527966 1101 58-59 0.24196805528438858 1101 60-61 0.24053733873167005 1101 62-63 0.3355543000870185 1101 64-65 0.4261180815304897 1101 66-67 0.3632412351185792 1101 68-69 0.3656794683803355 1101 70-71 0.362944636392065 1101 72-73 0.20618446512924038 1101 74-75 0.12924298475423512 1101 76-77 0.0971672601937641 1101 78-79 0.07482263110975396 1101 80-81 0.21813986161627952 1101 82-83 0.13527775888491433 1101 84-85 0.27426741131520416 1101 86-87 0.25781284253020686 1101 88-89 0.2044117651715638 1101 90-91 0.2295361847155064 1101 92-93 0.2033915226716978 1101 94-95 0.2149031267106416 1101 96-97 0.23339750978258067 1101 98-99 0.3338007136552541 1101 100-101 0.3702761879125198 1102 1 0.016231154915995916 1102 2 0.028982770605260555 1102 3 -0.006790488376559267 1102 4 0.05906856016374462 1102 5 0.08023611892863869 1102 6 0.06104719968200811 1102 7 0.0716813036248638 1102 8 0.0812925784561287 1102 9 0.05924896145970848 1102 10-11 0.04344937211267563 1102 12-13 0.032888795980973384 1102 14-15 0.2137130238625886 1102 16-17 0.20783089378858932 1102 18-19 0.2195437266757807 1102 20-21 0.25066228090073395 1102 22-23 0.2692752094692423 1102 24-25 0.14851277001186247 1102 26-27 0.2622317961642153 1102 28-29 0.21159810312542504 1102 30-31 0.15707108705451134 1102 32-33 0.21159478510488583 1102 34-35 0.220011441960267 1102 36-37 0.2656397089040894 1102 38-39 0.19092788397455962 1102 40-41 0.20723134265762155 1102 42-43 0.26108188321075687 1102 44-45 0.31014253110351575 1102 46-47 0.10460970370012745 1102 48-49 0.2922905349578784 1102 50-51 0.17583800607867062 1102 52-53 0.2562933498994724 1102 54-55 0.36352504649509854 1102 56-57 0.3520475130352736 1102 58-59 0.33298223284826634 1102 60-61 0.40932622089678006 1102 62-63 0.38674444031392596 1102 64-65 0.335519752370395 1102 66-67 0.4115306493188484 1102 68-69 0.3717178832452106 1102 70-71 0.37421397623538866 1102 72-73 0.20975642419375617 1102 74-75 0.2293598372830914 1102 76-77 0.13467098818890122 1102 78-79 0.20277899427225776 1102 80-81 0.26029261123165526 1102 82-83 0.3143161552633238 1102 84-85 0.36980065585320077 1102 86-87 0.18320905068568294 1102 88-89 0.25919180150068755 1102 90-91 0.2552715353913797 1102 92-93 0.24960680125296264 1102 94-95 0.21640243682955074 1102 96-97 0.19095327522481753 1102 98-99 0.22110275824485726 1102 100-101 0.3403988035631045 1103 1 0.022665422312396544 1103 2 0.02235196585077759 1103 3 0.012824133712253882 1103 4 0.03844981745064047 1103 5 0.01058318234808553 1103 6 0.005315356561204965 1103 7 0.06299805790787616 1103 8 0.0870536781547031 1103 9 -0.006504349401602383 1103 10-11 0.052383103057749736 1103 12-13 0.08219890504891225 1103 14-15 0.21438274034988325 1103 16-17 0.19936991813985117 1103 18-19 0.23383918565772177 1103 20-21 0.17911093633333053 1103 22-23 0.17763685080200275 1103 24-25 0.1861404103090223 1103 26-27 0.19119546908536478 1103 28-29 0.18868818044707325 1103 30-31 0.22397734442617434 1103 32-33 0.1754455344171859 1103 34-35 0.2158730166907148 1103 36-37 0.15648391375245296 1103 38-39 0.18935146787627133 1103 40-41 0.18005529991976488 1103 42-43 0.15314218566758342 1103 44-45 0.23730739676302903 1103 46-47 0.0693646096261844 1103 48-49 0.11374754643721019 1103 50-51 0.13187691006139346 1103 52-53 0.23290843166530806 1103 54-55 0.3525710253383352 1103 56-57 0.4166238400075528 1103 58-59 0.3235429903805027 1103 60-61 0.36494389555103623 1103 62-63 0.37677237937693775 1103 64-65 0.3806745434014118 1103 66-67 0.46129153492127273 1103 68-69 0.42105269571490567 1103 70-71 0.4414471903949888 1103 72-73 0.2556675147133092 1103 74-75 0.24368805249137182 1103 76-77 0.3206934765189615 1103 78-79 0.39069534952267304 1103 80-81 0.4044151028070715 1103 82-83 0.41397530886665734 1103 84-85 0.4534823999908646 1103 86-87 0.40720254448779514 1103 88-89 0.3527470957060963 1103 90-91 0.40820278776181596 1103 92-93 0.24678692005210578 1103 94-95 0.33807183701642174 1103 96-97 0.39106602687861525 1103 98-99 0.43037350898123705 1103 100-101 0.3886797253196832 1104 1 0.06760298043010948 1104 2 0.044928639947730886 1104 3 0.06540209987181811 1104 4 0.078728753460517 1104 5 0.043479230220903275 1104 6 0.06799947269330886 1104 7 0.13229579398657165 1104 8 0.04761815330614638 1104 9 0.1622142012031773 1104 10-11 0.07905485131664847 1104 12-13 0.03551976815565894 1104 14-15 0.17291514579332556 1104 16-17 0.148863966283578 1104 18-19 0.18171743029136422 1104 20-21 0.1801005692603539 1104 22-23 0.14767399704408035 1104 24-25 0.1831836734298733 1104 26-27 0.13792711973216853 1104 28-29 0.12389567588301276 1104 30-31 0.040345847376386246 1104 32-33 0.11360251422195233 1104 34-35 0.13422548289011615 1104 36-37 0.12992000474311283 1104 38-39 0.12813537140940667 1104 40-41 0.14889901769490876 1104 42-43 0.04367882010559043 1104 44-45 0.002173452939409515 1104 46-47 -0.04996546149082093 1104 48-49 -0.10602399056163847 1104 50-51 -0.06632418285843045 1104 52-53 0.046168787636368336 1104 54-55 0.09796902859950762 1104 56-57 0.1242995008283998 1104 58-59 0.14332268366403156 1104 60-61 0.12933505177301186 1104 62-63 0.12126800298906204 1104 64-65 0.12337026131848461 1104 66-67 0.14821773583098263 1104 68-69 0.12117776804474545 1104 70-71 0.02349980479106506 1104 72-73 -0.09525309105205082 1104 74-75 -0.12455893989259437 1104 76-77 -0.08913793162620465 1104 78-79 0.03777101462807764 1104 80-81 0.12236227083567996 1104 82-83 0.03133534713313324 1104 84-85 0.1330138019100353 1104 86-87 0.07315950652949255 1104 88-89 0.05110196973891945 1104 90-91 0.15792769446663968 1104 92-93 0.20984344143127842 1104 94-95 0.21288645169501308 1104 96-97 0.10624725483795672 1104 98-99 0.10409350227768144 1104 100-101 0.22092511701883666 1105 1 -0.09488224251449395 1105 2 -0.08068806147985796 1105 3 0.0406989835058873 1105 4 -0.019886861129940314 1105 5 -0.002822721965678454 1105 6 -0.04923335163108078 1105 7 -0.06005552481894938 1105 8 -0.09644046154370756 1105 9 -0.11894895496507729 1105 10-11 -0.07136050561470597 1105 12-13 -0.048450898786960295 1105 14-15 0.018238234902007378 1105 16-17 -0.07223600059548829 1105 18-19 0.006677972733356796 1105 20-21 -0.01491061951661976 1105 22-23 -0.03983151737539714 1105 24-25 -0.08688661562474209 1105 26-27 -0.051216938564920156 1105 28-29 -0.05694781077863098 1105 30-31 -0.09632725847282586 1105 32-33 -0.08806342036636039 1105 34-35 -0.04569083986566369 1105 36-37 -0.06759765649268701 1105 38-39 -0.0048121052610881065 1105 40-41 0.06297204921001054 1105 42-43 0.07765307241385955 1105 44-45 0.010571576177909492 1105 46-47 0.017356054293735212 1105 48-49 0.07154148155158424 1105 50-51 0.005020462718370311 1105 52-53 0.09856183649679906 1105 54-55 0.21864597529152263 1105 56-57 0.1409272298239017 1105 58-59 0.0012803735954634021 1105 60-61 0.007500672787465135 1105 62-63 -0.023467621045163867 1105 64-65 0.047705779730051745 1105 66-67 0.10471525514985558 1105 68-69 -0.02638359546186564 1105 70-71 -0.027864874347308444 1105 72-73 -0.03142598278554942 1105 74-75 0.04084284825687945 1105 76-77 -0.06189598447925704 1105 78-79 -0.016982311685637796 1105 80-81 -0.0027293890106747654 1105 82-83 0.06403014820466169 1105 84-85 0.06520532250438649 1105 86-87 0.08668051883762473 1105 88-89 0.14046829422647633 1105 90-91 0.15866994989147543 1105 92-93 0.1623427484547726 1105 94-95 0.08547161260272773 1105 96-97 0.09767232593888409 1105 98-99 0.010812562240356982 1105 100-101 0.05051133722469103 1106 1 0.16417078965729104 1106 2 0.0900908191103511 1106 3 0.11013996631102785 1106 4 0.15047640917201477 1106 5 0.14523363786484111 1106 6 0.12161397864945656 1106 7 0.13916094513761834 1106 8 0.17091121459806402 1106 9 0.14114983043201335 1106 10-11 0.22229448573457944 1106 12-13 0.19308557998584064 1106 14-15 0.2544095287036683 1106 16-17 0.3021226706561393 1106 18-19 0.23501802396063454 1106 20-21 0.3164477640540966 1106 22-23 0.32856898742238627 1106 24-25 0.30501583940188937 1106 26-27 0.29169251712441735 1106 28-29 0.25401811793415874 1106 30-31 0.3358646736045827 1106 32-33 0.3311422385969891 1106 34-35 0.2924449173342083 1106 36-37 0.2813859350166439 1106 38-39 0.28166341057299604 1106 40-41 0.3790715979119099 1106 42-43 0.364772880301949 1106 44-45 0.37711737201073703 1106 46-47 0.3299724107583657 1106 48-49 0.38708617129722 1106 50-51 0.40515012202258305 1106 52-53 0.45600916280490367 1106 54-55 0.5090477909916444 1106 56-57 0.4687078623417591 1106 58-59 0.49921380162643914 1106 60-61 0.4462648963875111 1106 62-63 0.532874216067647 1106 64-65 0.5528271193534948 1106 66-67 0.5471402557531917 1106 68-69 0.6266290131633099 1106 70-71 0.7315791005956171 1106 72-73 0.48549913752465557 1106 74-75 0.5642082194624365 1106 76-77 0.5680283086682252 1106 78-79 0.5967304571822964 1106 80-81 0.6305896777413409 1106 82-83 0.6522767490528345 1106 84-85 0.7186048160407488 1106 86-87 0.7375539945505807 1106 88-89 0.7407804611215596 1106 90-91 0.700934002954618 1106 92-93 0.7074646561786082 1106 94-95 0.7540047880639484 1106 96-97 0.7863060039126708 1106 98-99 0.8511569699006536 1106 100-101 0.8824671872812075 1107 1 0.008078182070160977 1107 2 -0.031230566256159165 1107 3 0.037436419514421004 1107 4 0.02808927384044324 1107 5 0.02075747596241939 1107 6 -0.08525568055154054 1107 7 -0.12080071437183904 1107 8 -0.08463863767899227 1107 9 -0.13338049253420792 1107 10-11 -0.097885812169352 1107 12-13 -0.14047939883170812 1107 14-15 -0.06112761771314723 1107 16-17 -0.030298238526782484 1107 18-19 -0.014286738357242257 1107 20-21 5.742949023996857E-4 1107 22-23 -0.09605751812264884 1107 24-25 0.0035143308445597654 1107 26-27 -0.08372709556673641 1107 28-29 -0.057527285785660354 1107 30-31 0.03277508313210831 1107 32-33 -0.06823219810384984 1107 34-35 0.0025985805258912364 1107 36-37 -0.007991989921777076 1107 38-39 0.053012750036955936 1107 40-41 0.07182699835436068 1107 42-43 0.056441745943963895 1107 44-45 -0.013719768863445836 1107 46-47 -0.1351818127201838 1107 48-49 -0.10556172033727762 1107 50-51 -0.17336399118186563 1107 52-53 -0.06336983451053868 1107 54-55 0.007176402733328757 1107 56-57 0.017122973459294144 1107 58-59 0.028562892877289414 1107 60-61 -0.021737451997658752 1107 62-63 -0.0016150553425475778 1107 64-65 0.036273805347050825 1107 66-67 0.07055723449362006 1107 68-69 0.03656862267348515 1107 70-71 0.06039850989662199 1107 72-73 -0.023128677766237615 1107 74-75 0.06677484540287537 1107 76-77 -0.01776772642862312 1107 78-79 0.0853570856289565 1107 80-81 0.06264811746893173 1107 82-83 0.1359324883182147 1107 84-85 0.2130713807713569 1107 86-87 0.2334601350536616 1107 88-89 0.10525640613873932 1107 90-91 0.1391318010874727 1107 92-93 0.19623286248948446 1107 94-95 0.25071686808978555 1107 96-97 0.28344763720310695 1107 98-99 0.29017863071142 1107 100-101 0.27997701338744463 1108 1 0.083977618626637 1108 2 0.1198824700743586 1108 3 0.11640994563524387 1108 4 0.1716773879341389 1108 5 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64-65 -1.3177552001394162 2316 66-67 -1.4112808712676923 2316 68-69 -1.4249121329928016 2316 70-71 -1.3509023893420817 2316 72-73 -1.0177576549343286 2316 74-75 -1.1377873881351164 2316 76-77 -1.167969277684879 2316 78-79 -1.1278936336230174 2316 80-81 -1.079607436754678 2316 82-83 -1.061877332945926 2316 84-85 -1.0208246065322868 2316 86-87 -1.0261195325268346 2316 88-89 -1.0876152247564335 2316 90-91 -1.015326583931234 2316 92-93 -1.073877418470488 2316 94-95 -1.0532350653776703 2316 96-97 -1.0207821811770792 2316 98-99 -1.015354766729157 2316 100-101 -1.1224557825916932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96176.0 3 14925.0 4 11113.0 5 9388.0 6 9429.0 7 9994.0 8 10053.0 9 10667.0 10 10934.0 11 10971.0 12 11471.0 13 12228.0 14 12705.0 15 14275.0 16 16697.0 17 20521.0 18 18283.0 19 14978.0 20 15489.0 21 16657.0 22 18758.0 23 21507.0 24 25340.0 25 29599.0 26 33652.0 27 39679.0 28 47700.0 29 58422.0 30 73206.0 31 91344.0 32 119825.0 33 160323.0 34 222611.0 35 327911.0 36 526570.0 37 1065499.0 38 1279504.0 39 909.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.17425787345229 24.35850172465833 13.753384440095829 22.713855961793556 2 13.327497045657813 22.40181058573384 44.63831166444464 19.6323807041637 3 16.417552382829857 31.643249901710846 31.25085063278169 20.68834708267761 4 10.520031791402777 18.93308123558784 44.53099661770838 26.015890355301003 5 11.143049349330173 39.31725333131368 37.65357901818133 11.886118301174815 6 24.40794393262399 42.82076121669396 21.398441142330686 11.372853708351368 7 22.930858240447925 33.47732715451117 28.33088269853316 15.260931906507743 8 22.026555065329596 38.8522252736666 22.89475026579791 16.226469395205903 9 25.31166795454004 16.288282861988016 24.4235365188393 33.97651266463265 10-11 21.70245780559349 28.426108736766757 32.130097067266135 17.74133639037362 12-13 22.113373534755503 26.384519404483157 31.555280036878713 19.946827023882623 14-15 19.93096696371589 28.14009540771036 28.570076105636804 23.358861522936945 16-17 18.817010342290786 30.364377655394716 30.34442930280452 20.47418269950998 18-19 18.684667917465124 31.001435132511684 31.265740095664363 19.048156854358833 20-21 19.313569728104245 29.593818652633335 30.74939648331454 20.343215135947876 22-23 19.47990705927611 30.34251565885471 29.959528328722012 20.218048953147175 24-25 19.295203887009425 30.299806484845952 30.578000529034234 19.82698909911039 26-27 19.558816683087148 29.87722174862835 30.159536659618073 20.404424908666428 28-29 19.379769243089086 30.72805349058976 29.723311785121687 20.16886548119946 30-31 20.568013413188165 30.074345451074585 29.341939401418436 20.015701734318814 32-33 20.28685680860301 30.30471477484417 29.473028501242844 19.935399915309983 34-35 20.48511654233064 29.462280754315863 30.198150585624123 19.85445211772937 36-37 20.709872535062715 29.814472726673323 30.229558509286385 19.246096228977574 38-39 21.119757076416814 29.271371811232587 30.78757261968591 18.82129849266469 40-41 20.179147997295797 29.511472265751603 30.74961491812207 19.559764818830534 42-43 20.715477035737656 29.481552019379357 29.90268554483565 19.900285400047334 44-45 20.039540800131515 30.531585527946863 29.86816169249148 19.56071197943014 46-47 20.96022118727702 29.419602206262297 29.3179292193981 20.302247387062586 48-49 20.747014110229202 28.563464119215006 30.28422568976531 20.40529608079048 50-51 20.65868471064981 29.217528692120627 29.148517300711852 20.975269296517716 52-53 19.658815234314186 29.595561138008236 29.87911436889122 20.866509258786355 54-55 20.398538833236728 30.052703514986863 29.1452684003858 20.403489251390617 56-57 20.66406442873609 29.204408686449245 29.690144375723182 20.441382509091486 58-59 20.374326347631488 28.65106653316593 30.700875503713736 20.273731615488842 60-61 20.305924314216107 30.374772417961577 29.118003590385165 20.201299677437152 62-63 20.66238998630067 29.57884752982678 28.60834715420361 21.150415329668938 64-65 19.70186398939024 29.503420075910363 29.06419057905662 21.73052535564278 66-67 20.36686614510842 28.23686850669199 29.72763627816663 21.668629070032956 68-69 19.458469886731336 29.17099858680699 29.236483934711575 22.134047591750097 70-71 19.831662034279883 28.779291835454586 29.912211302041218 21.47683482822431 72-73 20.50067272261133 28.65856719348392 29.918182870722603 20.92257721318215 74-75 20.228537437221465 29.480292447830088 29.1269666986674 21.164203416281048 76-77 19.797662641119363 29.34204433657941 30.18898188711079 20.67131113519044 78-79 19.615947304163605 29.085138376085773 29.727249285521957 21.571665034228666 80-81 19.739155139358314 29.236394048435532 30.24972318707504 20.774727625131117 82-83 20.46096399322667 28.33389926681828 30.26368404827115 20.941452691683907 84-85 21.27670624437931 28.24226528012881 29.381112956194677 21.09991551929721 86-87 20.056389397024702 29.32930874138379 29.502936543002356 21.11136531858915 88-89 20.772646937046346 28.296606236720606 29.697751262784735 21.232995563448316 90-91 20.68205289931334 27.76713652940052 30.34452853214501 21.206282039141133 92-93 20.17606408890004 28.742042802433787 29.737538058588907 21.34435505007727 94-95 19.48319645290377 28.187716056883964 30.119173515151697 22.20991397506057 96-97 19.910848611287097 28.204823330162633 29.430922620056787 22.453405438493483 98-99 19.368087646406977 28.92368688010106 29.721271503829634 21.986953969662334 100-101 20.050902125677407 27.637743824684485 29.53651781897877 22.774836230659336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 38.0 1 29.5 2 35.0 3 51.0 4 44.0 5 44.5 6 63.5 7 113.0 8 210.0 9 251.5 10 225.0 11 259.5 12 377.0 13 573.0 14 762.0 15 953.5 16 1211.0 17 1461.0 18 1614.5 19 1693.0 20 1836.0 21 2034.5 22 2310.5 23 2675.0 24 3188.5 25 3873.0 26 5368.5 27 8777.5 28 17186.5 29 36077.5 30 62661.5 31 92995.5 32 131928.0 33 178869.5 34 239200.0 35 284975.5 36 317381.0 37 359596.5 38 356243.5 39 296433.0 40 238359.0 41 192336.5 42 157811.5 43 135994.5 44 116578.0 45 104409.5 46 96630.0 47 91839.0 48 89353.5 49 85061.0 50 79954.5 51 77408.0 52 73694.0 53 70134.0 54 67179.5 55 61171.0 56 55441.5 57 48108.0 58 40767.0 59 37266.5 60 31842.0 61 25265.0 62 19988.5 63 16296.5 64 13141.5 65 9799.0 66 7435.0 67 5833.5 68 4755.0 69 3962.0 70 3279.5 71 2645.0 72 2123.0 73 1742.0 74 1451.5 75 1177.5 76 996.0 77 828.5 78 661.5 79 554.0 80 443.5 81 348.5 82 304.5 83 249.5 84 204.0 85 170.5 86 149.0 87 129.5 88 106.5 89 78.0 90 61.5 91 55.0 92 40.5 93 30.0 94 19.5 95 14.5 96 12.0 97 9.0 98 7.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.0095221696504564E-4 2 0.003519469460026512 3 1.7820098531779808E-4 4 0.0010914810350715132 5 0.0020715864543194022 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.796293107653665E-5 12-13 5.56878079118119E-5 14-15 0.003519469460026512 16-17 0.00491166465782181 18-19 0.004020659731232818 20-21 1.113756158236238E-5 22-23 0.0 24-25 1.113756158236238E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.7820098531779808E-4 42-43 1.113756158236238E-5 44-45 5.791532022828437E-4 46-47 0.7431092463368003 48-49 0.5331662105092695 50-51 0.4079020553924398 52-53 0.34244660597289606 54-55 0.33286830301206444 56-57 0.2989767031169357 58-59 0.29921059191016536 60-61 0.2752314218233391 62-63 0.2692282761304458 64-65 0.2656753939856722 66-67 0.25361341479197375 68-69 0.23274162438662663 70-71 0.24636286220185585 72-73 0.8215845052461257 74-75 0.541541656819206 76-77 0.6369460093337221 78-79 0.35512115105362446 80-81 0.30778651432858434 82-83 0.2880841678893853 84-85 0.27957507084046046 86-87 0.2526778596190553 88-89 0.2564089427491467 90-91 0.2667891501439084 92-93 0.22353086095801294 94-95 0.1910648689454266 96-97 0.14703808801034812 98-99 0.15659411584801505 100-101 0.1512258111653164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4489313.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.672117352078786 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74677649982287 30.843792410468147 2 11.972919259418033 9.499821158131928 3 4.258717935673027 5.0685707314026915 4 1.9434522629765634 3.084034649798772 5 1.0745588766268648 2.1315012927629464 6 0.6531715143843719 1.5547618181815093 7 0.4350702268032535 1.2082109965893946 8 0.2822972448548926 0.8959463540841456 9 0.214081018266357 0.7643742551563893 >10 1.1190769464815131 8.324100947011571 >50 0.13485656621865205 3.7698941507929984 >100 0.1329404626098079 11.014432149679127 >500 0.017140036041855405 4.812613951004704 >1k 0.01324969889867427 10.319815596253898 >5k 0.001014870553941008 2.8029330366413268 >10k+ 6.765803692940053E-4 3.9051965020405968 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 19926 0.4438541041803056 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 19731 0.4395104551631842 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 17258 0.3844240755768199 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 15853 0.3531275275303816 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 13790 0.30717394844155443 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 13717 0.3055478644505295 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 12789 0.284876550153665 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 12690 0.2826713129603572 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 12499 0.27841676443589475 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 12446 0.2772361829081643 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 12296 0.2738949144334556 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 11595 0.2582800530949836 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 9967 0.22201615258281168 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 9872 0.21990001588216282 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 9558 0.21290562720843925 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 8430 0.18777928827862972 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 8121 0.18089627522072976 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7609 0.16949141216039068 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 7225 0.1609377648651364 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 6851 0.15260686880152932 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6509 0.14498877667919347 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 6217 0.13848444071509383 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 6164 0.1373038591873634 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 5836 0.1299976187893337 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 5769 0.12850518553729715 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 5648 0.12580989563436545 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 5595 0.12462931410663503 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 5542 0.12344873257890462 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 5396 0.1201965645968548 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 5002 0.11142016606995324 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 4940 0.1100391084337403 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 4707 0.10484900473635944 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 4560 0.1015745616311449 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 4520 0.10068355670455592 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.227512316472476E-5 0.0 5 0.0 0.0 0.0 2.227512316472476E-5 0.0 6 4.455024632944952E-5 0.0 0.0 2.227512316472476E-5 0.0 7 4.455024632944952E-5 0.0 0.0 2.227512316472476E-5 0.0 8 4.455024632944952E-5 0.0 0.0 2.227512316472476E-5 0.0 9 4.455024632944952E-5 0.0 0.0 8.910049265889904E-5 2.227512316472476E-5 10-11 4.455024632944952E-5 0.0 0.0 8.910049265889904E-5 2.227512316472476E-5 12-13 4.455024632944952E-5 0.0 0.0 8.910049265889904E-5 2.227512316472476E-5 14-15 4.455024632944952E-5 2.227512316472476E-5 0.0 1.7820098531779805E-4 2.227512316472476E-5 16-17 8.910049265889904E-5 4.455024632944952E-5 0.0 2.0047610848252282E-4 2.227512316472476E-5 18-19 8.910049265889904E-5 4.455024632944952E-5 0.0 2.116136700648852E-4 4.455024632944952E-5 20-21 8.910049265889904E-5 4.455024632944952E-5 0.0 2.6730147797669713E-4 4.455024632944952E-5 22-23 8.910049265889904E-5 4.455024632944952E-5 0.0 3.22989285888509E-4 4.455024632944952E-5 24-25 8.910049265889904E-5 4.455024632944952E-5 0.0 5.457405175357565E-4 4.455024632944952E-5 26-27 8.910049265889904E-5 4.455024632944952E-5 0.0 0.0011583064045656874 4.455024632944952E-5 28-29 8.910049265889904E-5 4.455024632944952E-5 0.0 0.002884628449831856 4.455024632944952E-5 30-31 8.910049265889904E-5 4.455024632944952E-5 0.0 0.010892535227550407 4.455024632944952E-5 32-33 8.910049265889904E-5 4.455024632944952E-5 0.0 0.03780088401053792 4.455024632944952E-5 34-35 8.910049265889904E-5 4.455024632944952E-5 0.0 0.08276322011853485 4.455024632944952E-5 36-37 8.910049265889904E-5 5.56878079118119E-5 0.0 0.15671662902542105 6.682536949417428E-5 38-39 8.910049265889904E-5 6.682536949417428E-5 0.0 0.2594160843763845 6.682536949417428E-5 40-41 1.113756158236238E-4 7.796293107653667E-5 0.0 0.36493334280768575 8.910049265889904E-5 42-43 1.3365073898834856E-4 8.910049265889904E-5 0.0 0.4843836907785222 8.910049265889904E-5 44-45 1.3365073898834856E-4 8.910049265889904E-5 0.0 0.631132202187729 8.910049265889904E-5 46-47 1.3365073898834856E-4 8.910049265889904E-5 0.0 0.80948911336768 8.910049265889904E-5 48-49 1.3365073898834856E-4 8.910049265889904E-5 0.0 0.9911316052144281 8.910049265889904E-5 50-51 1.3365073898834856E-4 1.113756158236238E-4 0.0 1.1759483021121495 8.910049265889904E-5 52-53 1.3365073898834856E-4 1.113756158236238E-4 0.0 1.3668349700722584 1.0023805424126141E-4 54-55 1.3365073898834856E-4 1.670634237354357E-4 0.0 1.5734924252329923 1.113756158236238E-4 56-57 1.3365073898834856E-4 1.7820098531779808E-4 0.0 1.8241989364519693 1.113756158236238E-4 58-59 1.3365073898834856E-4 1.7820098531779808E-4 0.0 2.113385722938008 1.113756158236238E-4 60-61 1.3365073898834856E-4 1.7820098531779808E-4 0.0 2.410825442556578 1.113756158236238E-4 62-63 1.559258621530733E-4 1.7820098531779808E-4 0.0 2.704478391237145 1.113756158236238E-4 64-65 1.559258621530733E-4 1.7820098531779808E-4 0.0 3.017789136110581 1.113756158236238E-4 66-67 1.559258621530733E-4 1.7820098531779808E-4 0.0 3.363588148119768 1.113756158236238E-4 68-69 1.559258621530733E-4 1.7820098531779808E-4 0.0 3.7461299757891684 1.113756158236238E-4 70-71 1.559258621530733E-4 1.7820098531779808E-4 0.0 4.143830024772164 1.113756158236238E-4 72-73 1.559258621530733E-4 1.7820098531779808E-4 0.0 4.580778840771405 1.113756158236238E-4 74-75 1.559258621530733E-4 1.7820098531779808E-4 0.0 5.000074621662602 1.113756158236238E-4 76-77 1.559258621530733E-4 1.7820098531779808E-4 0.0 5.471315989773936 1.113756158236238E-4 78-79 1.559258621530733E-4 1.7820098531779808E-4 0.0 5.985871334879079 1.113756158236238E-4 80-81 1.559258621530733E-4 1.7820098531779808E-4 0.0 6.566717446522441 1.113756158236238E-4 82-83 1.559258621530733E-4 1.7820098531779808E-4 0.0 7.1959339881180036 1.113756158236238E-4 84-85 1.559258621530733E-4 1.7820098531779808E-4 0.0 7.7910584537099545 1.113756158236238E-4 86-87 1.559258621530733E-4 1.7820098531779808E-4 0.0 8.437337739649697 1.113756158236238E-4 88-89 1.559258621530733E-4 1.7820098531779808E-4 0.0 9.11511404974436 1.113756158236238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 4950 0.0 53.953396 3 TACCTGG 4965 0.0 53.697414 2 GTACCTG 5505 0.0 49.032787 1 CCTGGGG 4305 0.0 45.757793 4 CTGGGGG 3765 0.0 42.258526 5 GTACCCG 415 0.0 36.514973 1 TGGGGGG 7815 0.0 32.416767 6 TATTCGC 780 0.0 27.321003 9 GTACATG 11790 0.0 26.991346 1 CATGGGG 7360 0.0 26.828917 4 GAACCGT 565 0.0 25.981136 6 GGGGGGA 7955 0.0 25.953217 7 GCATCGT 1435 0.0 25.739042 8 ACATGGG 11645 0.0 25.374062 3 ATGGGGG 6345 0.0 25.373907 5 GTATCAA 8685 0.0 25.190758 1 TACATGG 12280 0.0 25.027103 2 GTACACG 500 0.0 24.62478 1 TGAACCG 540 0.0 24.552984 5 CTAACGC 800 0.0 24.268267 3 >>END_MODULE