FastQCFastQC Report
Sat 18 Jun 2016
SRR1931094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931372
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA184680.4697596665998537No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC138350.3519127673494139No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC124470.31660702675808855No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA104700.2663192391867267No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC104450.26568332887348234No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC103510.26329230609568366No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC98080.24948033409201675No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG93940.2389496593046906No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA83120.21142746094747586No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC78450.19954865629607169No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA76380.19428331890240863No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG74260.18889079944609669No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA67110.17070376448730876No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT58610.14908281383700142No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC55800.14193518191613513No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC52760.13420251250708404No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT49650.12629178821032455No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA46020.11705837046201682No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA45110.11474365692180745No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA44500.11319203575749129No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC44410.11296310804472331No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG43840.11151323253052624No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG41320.10510325657302336No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG41280.10500151092290427No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA40430.10283941585787354No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA39870.10141497675620623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG46300.057.0624432
ACCTGGG48350.054.935433
GTACCTG53800.049.2836571
CCTGGGG39950.045.746374
CTGGGGG35050.042.6859745
GTACCCG4000.041.4290731
TGGGGGG59650.031.8283796
GGGGGGA59200.028.6313577
GGGGAAT60850.026.99989
GTGATCG8250.025.2510438
GTATCAA77400.024.6525151
CATGGGG47850.024.24214
GTACACG4900.024.1568951
GGGGGAA68850.024.068268
GCATCGT13200.024.0315328
TATATCC61500.024.019594
GAACCGT4350.023.9449566
ATGGGGG40500.023.3808945
ATCAACG78150.023.2642143
CAACGCA77750.023.262115
Produced by FastQC (version 0.11.5)