##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931094_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3931372 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.378982451927723 33.0 33.0 33.0 27.0 33.0 2 31.31304160481379 33.0 33.0 33.0 27.0 33.0 3 31.33146799641448 33.0 33.0 33.0 27.0 33.0 4 35.000500588598584 37.0 37.0 37.0 33.0 37.0 5 34.99107309102268 37.0 37.0 37.0 33.0 37.0 6 35.07619706301006 37.0 37.0 37.0 33.0 37.0 7 35.046345143629246 37.0 37.0 37.0 33.0 37.0 8 35.04498938284141 37.0 37.0 37.0 33.0 37.0 9 35.057849524288216 37.0 37.0 37.0 33.0 37.0 10-11 34.975474846949105 37.0 37.0 37.0 33.0 37.0 12-13 34.907943460959686 37.0 37.0 37.0 33.0 37.0 14-15 36.66730266176795 40.0 37.0 40.0 33.0 40.0 16-17 36.62684897282679 40.0 37.0 40.0 33.0 40.0 18-19 36.57734106566359 40.0 37.0 40.0 33.0 40.0 20-21 36.50247737939834 40.0 37.0 40.0 33.0 40.0 22-23 36.431773055309954 40.0 37.0 40.0 33.0 40.0 24-25 36.33077523571923 40.0 37.0 40.0 33.0 40.0 26-27 36.18691210091541 40.0 37.0 40.0 33.0 40.0 28-29 36.08964465840424 40.0 37.0 40.0 33.0 40.0 30-31 35.97337799628221 40.0 37.0 40.0 33.0 40.0 32-33 35.84242371365518 40.0 37.0 40.0 33.0 40.0 34-35 35.74585081238814 40.0 37.0 40.0 27.0 40.0 36-37 35.56575567003072 40.0 37.0 40.0 27.0 40.0 38-39 35.41450134965605 38.5 37.0 40.0 27.0 40.0 40-41 35.225773597614264 37.0 37.0 40.0 27.0 40.0 42-43 35.08330348794263 37.0 37.0 40.0 27.0 40.0 44-45 34.87770897793442 37.0 37.0 40.0 27.0 40.0 46-47 34.72557570232479 37.0 37.0 40.0 22.0 40.0 48-49 34.74661415912816 37.0 37.0 40.0 22.0 40.0 50-51 33.97325590150207 37.0 35.0 38.5 22.0 40.0 52-53 34.09640336757752 37.0 35.0 38.5 22.0 40.0 54-55 34.63150332759149 37.0 37.0 40.0 22.0 40.0 56-57 34.623086673049514 37.0 37.0 40.0 22.0 40.0 58-59 34.51003186673762 37.0 37.0 40.0 22.0 40.0 60-61 34.54698003648599 37.0 37.0 40.0 22.0 40.0 62-63 34.405672879595215 37.0 37.0 40.0 22.0 40.0 64-65 34.22182268683808 37.0 33.0 40.0 22.0 40.0 66-67 34.00872888650578 37.0 33.0 40.0 22.0 40.0 68-69 33.808761801223596 37.0 33.0 40.0 22.0 40.0 70-71 33.59365839711938 37.0 33.0 40.0 22.0 40.0 72-73 33.003324157571456 37.0 33.0 40.0 15.0 40.0 74-75 32.834575435751184 37.0 33.0 38.5 15.0 40.0 76-77 32.466599192343025 37.0 33.0 37.0 15.0 40.0 78-79 32.201561566801615 37.0 33.0 37.0 15.0 40.0 80-81 31.915330067976267 37.0 33.0 37.0 10.5 37.0 82-83 31.625482147199502 37.0 33.0 37.0 6.0 37.0 84-85 31.241959041271087 37.0 33.0 37.0 4.0 37.0 86-87 31.0620135922014 37.0 33.0 37.0 2.0 37.0 88-89 30.84877251504055 37.0 33.0 37.0 2.0 37.0 90-91 30.583046325811956 37.0 33.0 37.0 2.0 37.0 92-93 30.32278820218489 37.0 33.0 37.0 2.0 37.0 94-95 30.083531652563025 33.0 33.0 37.0 2.0 37.0 96-97 29.805762466640147 33.0 33.0 37.0 2.0 37.0 98-99 29.500453276871283 33.0 33.0 37.0 2.0 37.0 100-101 28.84152720729557 33.0 30.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.012817446237118446 1101 2 0.04815012684422726 1101 3 0.028027254745936148 1101 4 0.03198656329850991 1101 5 -0.04586524914478218 1101 6 -0.017970939225811833 1101 7 0.09774002425444195 1101 8 0.05109139350662417 1101 9 0.11860935182617993 1101 10-11 0.01418490087682045 1101 12-13 0.10925678228248614 1101 14-15 0.19388036855863078 1101 16-17 0.053184209434142815 1101 18-19 0.13398075677575605 1101 20-21 0.07423893656505953 1101 22-23 0.13557882614198746 1101 24-25 0.16023226245751943 1101 26-27 0.10762285427863105 1101 28-29 0.12954796453360728 1101 30-31 0.01002655315453893 1101 32-33 0.16350710568600135 1101 34-35 0.13315954799319485 1101 36-37 0.2484018344493677 1101 38-39 0.20769616097543064 1101 40-41 0.0765626937060091 1101 42-43 0.08396296476885112 1101 44-45 0.10297301536230918 1101 46-47 0.032857245819009506 1101 48-49 0.1406206450322074 1101 50-51 0.1602394258130886 1101 52-53 0.3724760028756222 1101 54-55 0.4483702390115667 1101 56-57 0.3724497856878557 1101 58-59 0.48540133043047007 1101 60-61 0.45934238830844976 1101 62-63 0.46602760173961855 1101 64-65 0.48625404476299394 1101 66-67 0.4057969140701445 1101 68-69 0.4063566156260663 1101 70-71 0.3498558312255895 1101 72-73 0.26167575895572526 1101 74-75 0.3013282314799355 1101 76-77 0.27771323853641405 1101 78-79 0.2906234597346966 1101 80-81 0.36515358553166877 1101 82-83 0.3253035307228913 1101 84-85 0.3657456144416109 1101 86-87 0.3383945691089032 1101 88-89 0.3379882109516288 1101 90-91 0.3124862780499029 1101 92-93 0.2978205747420617 1101 94-95 0.341585610634791 1101 96-97 0.29815440335245214 1101 98-99 0.42237560326170964 1101 100-101 0.4506690241194029 1102 1 0.16177403696212522 1102 2 0.10365254328827334 1102 3 0.11103690405877131 1102 4 0.1513946294570303 1102 5 0.0751975461257075 1102 6 0.16028852992749876 1102 7 0.15586956665007534 1102 8 0.10765235409223806 1102 9 0.16875637275141742 1102 10-11 0.1518939710874463 1102 12-13 0.188412908741185 1102 14-15 0.2905543084374287 1102 16-17 0.25586541991702205 1102 18-19 0.2371677016370839 1102 20-21 0.26351751770514653 1102 22-23 0.2479246065786782 1102 24-25 0.27521364502310064 1102 26-27 0.2883052468622367 1102 28-29 0.2101584889139403 1102 30-31 0.2821191387651538 1102 32-33 0.2906434487468843 1102 34-35 0.2844457834473886 1102 36-37 0.3004588070750742 1102 38-39 0.31092088187691047 1102 40-41 0.34238596443136515 1102 42-43 0.4152695952096437 1102 44-45 0.38319038366900315 1102 46-47 0.2927981717533541 1102 48-49 0.301699228812943 1102 50-51 0.15843501882030608 1102 52-53 0.27046423109539575 1102 54-55 0.4260805822788143 1102 56-57 0.455348959439867 1102 58-59 0.5134922785088278 1102 60-61 0.5736207371686746 1102 62-63 0.4797237187212957 1102 64-65 0.4454963462342221 1102 66-67 0.5118583425570407 1102 68-69 0.5000140959095916 1102 70-71 0.5336949885892466 1102 72-73 0.3539744428172966 1102 74-75 0.45215866865019194 1102 76-77 0.3054950098977187 1102 78-79 0.3638005803805271 1102 80-81 0.4136941671050174 1102 82-83 0.38109503371376263 1102 84-85 0.47970798119243696 1102 86-87 0.44897237381484345 1102 88-89 0.5434238937998082 1102 90-91 0.5410638402067676 1102 92-93 0.3985454346039603 1102 94-95 0.48669719543380907 1102 96-97 0.5652863877021694 1102 98-99 0.6555827475055445 1102 100-101 0.7001675033790953 1103 1 -0.07570999971242642 1103 2 -0.13552553273683188 1103 3 -0.14521570628739866 1103 4 -0.20138502970252858 1103 5 -0.2480806826153099 1103 6 -0.143012948372494 1103 7 -0.144761372633468 1103 8 -0.15677238756732947 1103 9 -0.18740727267757507 1103 10-11 -0.16341254181292442 1103 12-13 -0.18487028775714975 1103 14-15 -0.10823360424735284 1103 16-17 -0.08903096539724231 1103 18-19 -0.015646243005505767 1103 20-21 -0.03406683356372753 1103 22-23 -0.10608278207933353 1103 24-25 -0.14344438752219446 1103 26-27 -0.11064590837438715 1103 28-29 -0.18689059502933247 1103 30-31 -0.2122160263860593 1103 32-33 -0.22676291357473843 1103 34-35 -0.2368183777871522 1103 36-37 -0.25945302830550077 1103 38-39 -0.30180776284841215 1103 40-41 -0.283915684613973 1103 42-43 -0.3471930528255385 1103 44-45 -0.2879091822376836 1103 46-47 -0.4116963185831679 1103 48-49 -0.3251833181710211 1103 50-51 -0.22265551418026774 1103 52-53 -0.26213391962568267 1103 54-55 -0.15504630515634688 1103 56-57 -0.12940677809248058 1103 58-59 -0.1507758821682259 1103 60-61 -0.14121635108438824 1103 62-63 -0.14977775716871378 1103 64-65 -0.07616125683310315 1103 66-67 -0.1017704097764991 1103 68-69 -0.1564108058095286 1103 70-71 -0.16014940306283165 1103 72-73 -0.3036211217143219 1103 74-75 -0.1934868817511699 1103 76-77 -0.1848253661149002 1103 78-79 -0.17386775213318373 1103 80-81 -0.12266549670626858 1103 82-83 -0.11080377045497514 1103 84-85 -0.09374400537620531 1103 86-87 -0.19602213891636922 1103 88-89 -0.20730529794721164 1103 90-91 -0.18102219498405248 1103 92-93 -0.3164545551649596 1103 94-95 -0.21174924930028993 1103 96-97 -0.2354278780371537 1103 98-99 -0.1965699738591944 1103 100-101 -0.10139400939281629 1104 1 0.12221933868170254 1104 2 0.11415977449852122 1104 3 0.11744173562953009 1104 4 0.17803726702359057 1104 5 0.17250499199433733 1104 6 0.08335163038276505 1104 7 0.17785551404134736 1104 8 0.10256687869692627 1104 9 0.08660546560268756 1104 10-11 0.07229739242141875 1104 12-13 0.05781720809557811 1104 14-15 0.07354520789933616 1104 16-17 0.15908771722095594 1104 18-19 0.16588278633508224 1104 20-21 0.18331567119711423 1104 22-23 0.22834282425110786 1104 24-25 0.14510132440987888 1104 26-27 0.23504488822562308 1104 28-29 0.18029412989137228 1104 30-31 0.11811802043895625 1104 32-33 0.1277743514556775 1104 34-35 0.15190770347347637 1104 36-37 0.18509465303220907 1104 38-39 0.1775394061809834 1104 40-41 0.230447219589351 1104 42-43 0.12512219807546643 1104 44-45 0.20328224174788545 1104 46-47 0.11668985092071438 1104 48-49 0.21711108225846232 1104 50-51 0.21911584233894388 1104 52-53 0.2574928875764968 1104 54-55 0.34284804078804854 1104 56-57 0.34445552622497644 1104 58-59 0.27641757265084266 1104 60-61 0.23553326247368744 1104 62-63 0.2620870546909728 1104 64-65 0.31573293039885897 1104 66-67 0.32193362138371384 1104 68-69 0.39485138306640977 1104 70-71 0.36659949978923123 1104 72-73 0.3569176162989365 1104 74-75 0.32088405807288467 1104 76-77 0.3540789666418007 1104 78-79 0.3720134636783783 1104 80-81 0.4257001901789472 1104 82-83 0.30663372652274035 1104 84-85 0.3900410405550474 1104 86-87 0.36137826420750585 1104 88-89 0.3596738865094231 1104 90-91 0.3793525663669399 1104 92-93 0.3313536167827493 1104 94-95 0.335367130701858 1104 96-97 0.3284574138698062 1104 98-99 0.37878682734375957 1104 100-101 0.5840738312316098 1105 1 0.14399700896991519 1105 2 0.10250588846304609 1105 3 0.1170106266600861 1105 4 0.12175612305573225 1105 5 0.05314480129647592 1105 6 0.11352194787026093 1105 7 0.2015916198473633 1105 8 0.12499760901609847 1105 9 0.11757769044784538 1105 10-11 0.15479292649394694 1105 12-13 0.16248422498328097 1105 14-15 0.11489247724131246 1105 16-17 0.08038521781102759 1105 18-19 0.15875273540004997 1105 20-21 0.15678327984601736 1105 22-23 0.16215527810860664 1105 24-25 0.1528166451995574 1105 26-27 0.13842537414480205 1105 28-29 0.125268542568854 1105 30-31 0.09345725392058313 1105 32-33 0.1610795330822512 1105 34-35 0.26140829869355997 1105 36-37 0.18226868601701085 1105 38-39 0.1533692807938678 1105 40-41 0.215874193121131 1105 42-43 0.2201602008489374 1105 44-45 0.19844548204926582 1105 46-47 0.09538092448764957 1105 48-49 0.13097596367777697 1105 50-51 0.05534499736739207 1105 52-53 0.15060551396557287 1105 54-55 0.1937032901811051 1105 56-57 0.11584217614276326 1105 58-59 0.11928282073953511 1105 60-61 0.11913983116916427 1105 62-63 0.19716735068258373 1105 64-65 0.11374111761102768 1105 66-67 0.18400264525323706 1105 68-69 0.173537745503765 1105 70-71 0.21864705079042324 1105 72-73 0.1794424609078149 1105 74-75 0.1849412327746407 1105 76-77 0.10658982610006973 1105 78-79 0.16998618204143412 1105 80-81 0.21676169499090392 1105 82-83 0.2010534206147483 1105 84-85 0.2294007953380479 1105 86-87 0.16367476388561286 1105 88-89 0.25424097262230916 1105 90-91 0.23661373358855187 1105 92-93 0.3108660920366262 1105 94-95 0.3465690596657396 1105 96-97 0.3629250802571242 1105 98-99 0.3250720982881994 1105 100-101 0.46703945616328824 1106 1 0.12965304192699634 1106 2 0.13092221614768818 1106 3 0.13664100984703964 1106 4 0.15024506914349445 1106 5 0.09414264059358146 1106 6 0.10973045281546945 1106 7 0.20890700774806703 1106 8 0.1643651312293528 1106 9 0.22559987258742353 1106 10-11 0.15815156157201926 1106 12-13 0.0892432717601892 1106 14-15 0.2258127444743181 1106 16-17 0.12672390312066995 1106 18-19 0.17572559043735936 1106 20-21 0.25978033635745135 1106 22-23 0.23940690501534334 1106 24-25 0.24098624541217362 1106 26-27 0.19750930181224646 1106 28-29 0.19657550483450592 1106 30-31 0.23033259398000894 1106 32-33 0.19773462220368998 1106 34-35 0.18673367495020443 1106 36-37 0.30445043874972555 1106 38-39 0.177843582939488 1106 40-41 0.28143680216326317 1106 42-43 0.17586840435991036 1106 44-45 0.22841407900131827 1106 46-47 0.1641875401191868 1106 48-49 0.24126533308331943 1106 50-51 0.22509197381194923 1106 52-53 0.2549214428394393 1106 54-55 0.354730119830613 1106 56-57 0.32048029318965376 1106 58-59 0.3395224248212898 1106 60-61 0.21279507306072531 1106 62-63 0.30516720435989697 1106 64-65 0.265622948873407 1106 66-67 0.31477810731836087 1106 68-69 0.3672695624834006 1106 70-71 0.359278153578245 1106 72-73 0.17634046741049048 1106 74-75 0.35637105587688467 1106 76-77 0.1941473617626599 1106 78-79 0.2208430215107171 1106 80-81 0.30617089813152276 1106 82-83 0.4034147131870327 1106 84-85 0.42360795745360846 1106 86-87 0.3061779962529023 1106 88-89 0.22998802087085934 1106 90-91 0.28193033184121674 1106 92-93 0.34986573756380324 1106 94-95 0.3980975175623698 1106 96-97 0.37669461537107196 1106 98-99 0.4237216878211605 1106 100-101 0.4268124238880695 1107 1 0.10815939296264787 1107 2 0.10585125747487112 1107 3 0.09476151819526635 1107 4 0.09577042104071154 1107 5 0.1547109249278904 1107 6 0.10030516862673267 1107 7 0.09647532385062618 1107 8 0.1059674225243512 1107 9 0.10599915235385993 1107 10-11 0.1867887620972013 1107 12-13 0.1392983686323177 1107 14-15 0.1693758324661374 1107 16-17 0.23115697180785588 1107 18-19 0.28252405453789464 1107 20-21 0.2642351324900929 1107 22-23 0.212162946629455 1107 24-25 0.20167357139644793 1107 26-27 0.2484271492309773 1107 28-29 0.16567143514235028 1107 30-31 0.2784997761472212 1107 32-33 0.33346602504775547 1107 34-35 0.2865474925593432 1107 36-37 0.16788770276038179 1107 38-39 0.27417995671177664 1107 40-41 0.3492117599236053 1107 42-43 0.3091104007689509 1107 44-45 0.2642244942175225 1107 46-47 0.12250242170970438 1107 48-49 0.16082396356785722 1107 50-51 0.12483237995824936 1107 52-53 0.26663506209473553 1107 54-55 0.34239507394590163 1107 56-57 0.3641944653912006 1107 58-59 0.3958024411978869 1107 60-61 0.41957688115417824 1107 62-63 0.3123874335174648 1107 64-65 0.3894274130915747 1107 66-67 0.3822815358020861 1107 68-69 0.3440635535114893 1107 70-71 0.37708221948116716 1107 72-73 0.3328722950560916 1107 74-75 0.38419567801006593 1107 76-77 0.43256416459266234 1107 78-79 0.38496044514857175 1107 80-81 0.39196381529049873 1107 82-83 0.43573977439832845 1107 84-85 0.5525383381508568 1107 86-87 0.6177177640486384 1107 88-89 0.5120858880216268 1107 90-91 0.47089818632168345 1107 92-93 0.47147694821119757 1107 94-95 0.5190339079460067 1107 96-97 0.6587366010120768 1107 98-99 0.559104213979257 1107 100-101 0.5732178330140805 1108 1 0.016212829777323634 1108 2 0.024168919089362362 1108 3 0.04235267987760949 1108 4 0.0652460495065057 1108 5 -8.374022192825237E-4 1108 6 0.12688942453172558 1108 7 0.05110106207197873 1108 8 0.045213570893423594 1108 9 0.04281779860879453 1108 10-11 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-0.3638271711824572 2316 74-75 -0.5765922583284038 2316 76-77 -0.5843308115045325 2316 78-79 -0.6058686733961878 2316 80-81 -0.5797184162517262 2316 82-83 -0.597911640791537 2316 84-85 -0.646999488095684 2316 86-87 -0.6067887446757823 2316 88-89 -0.5982226510439084 2316 90-91 -0.6407917550088165 2316 92-93 -0.5564944497931528 2316 94-95 -0.5039838002015102 2316 96-97 -0.5928134598570267 2316 98-99 -0.5010051425160782 2316 100-101 -0.5488542245582977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94613.0 3 14179.0 4 10540.0 5 8921.0 6 8928.0 7 9473.0 8 9959.0 9 10147.0 10 10452.0 11 10637.0 12 11338.0 13 12426.0 14 13002.0 15 14167.0 16 17148.0 17 20944.0 18 18658.0 19 14574.0 20 14936.0 21 15798.0 22 17341.0 23 19968.0 24 23252.0 25 26571.0 26 29768.0 27 35359.0 28 42624.0 29 51600.0 30 63249.0 31 79949.0 32 103431.0 33 137612.0 34 191493.0 35 278393.0 36 449124.0 37 887769.0 38 1151598.0 39 1431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.393368998756664 24.549442532747005 13.849739224572104 22.207449243924227 2 13.344381642340036 22.634169545331982 44.81850417936417 19.20294463296381 3 16.095800773624035 32.02654445641936 31.29369504297106 20.583959726985547 4 10.366888186045754 19.005508054009148 44.51038756818932 26.117216191755777 5 10.836278723114706 39.207485593133036 37.810283406061885 12.145952277690373 6 24.239095155584362 42.72310023065739 21.657808012062965 11.379996601695286 7 22.64433383561769 33.73832341482821 28.270461304603074 15.346881444951025 8 21.851531729889718 38.80217898484295 23.20785211880229 16.138437166465042 9 24.852595989389965 16.48292250135576 24.622014909807568 34.042466599446705 10-11 21.578378328880476 28.603294957827046 32.08612619950218 17.732200513790293 12-13 21.966669358690076 26.67964773071965 31.752500879147018 19.60118203144326 14-15 19.773045903762316 28.394750200032536 28.929391281697576 22.902812614507575 16-17 18.706902253267465 30.650428115731152 30.308914880519332 20.333754750482044 18-19 18.56360782512155 30.985977474368266 31.65784432047076 18.79257038003943 20-21 19.235244082727355 29.674424094183916 31.00743201101295 20.082899812075784 22-23 19.342216915621314 30.48823667666148 30.225694744735428 19.94385166298178 24-25 19.228289669394 30.348378422135884 30.711864803415295 19.711467105054815 26-27 19.51526083006136 29.90808297968241 30.48451024222587 20.092145948030357 28-29 19.280800697568175 30.73546588824461 29.927338343967453 20.05639507021976 30-31 20.272998841117047 30.073432379332203 29.625649773158074 20.02791900639268 32-33 20.08661352830513 30.29358452977739 29.707198911728526 19.91260303018895 34-35 20.330853961416015 29.634895909112647 30.365798504949414 19.668451624521925 36-37 20.369402844604885 29.847264517323723 30.43881372711613 19.344518910955262 38-39 20.698359249646177 29.44875224221977 30.863907053313707 18.988981454820355 40-41 19.94160296528126 29.58890349507194 30.868662886468922 19.600830653177876 42-43 20.412161454067434 29.567972707746815 30.135331889223405 19.884533948962346 44-45 19.835402366798434 30.63543528174649 30.02649602445169 19.502666327003396 46-47 20.692435163016267 29.573996953340643 29.539063019611827 20.19450486403126 48-49 20.476319326940192 28.921919660851188 30.309225723422667 20.292535288785952 50-51 20.411005843870807 29.429279737846386 29.284667658504 20.87504675977881 52-53 19.582031894623764 29.809288523298395 29.888214003427336 20.720465578650504 54-55 20.04747338236364 30.28524866736345 29.34361668051307 20.32366126975984 56-57 20.427171847476206 29.550349855179554 29.71207367778624 20.310404619558007 58-59 20.427300357410395 28.993642748814587 30.300188502856628 20.278868390918387 60-61 20.304900267596672 30.39329861714723 29.307351759721378 19.994449355534723 62-63 20.48752211966139 29.78269644650629 28.871114512092085 20.85866692174024 64-65 19.72379373534737 29.70752192689636 29.27525219772715 21.293432140029118 66-67 20.306314710828445 28.542489884211005 29.890619923296995 21.260575481663555 68-69 19.636496132051935 29.459384792339144 29.285324260886558 21.61879481472236 70-71 20.124804614259233 28.905065895311367 29.901569830949814 21.068559659479583 72-73 20.726223264005654 28.803627499491462 30.083176796480032 20.38697244002285 74-75 20.426071380979142 29.611015892024366 29.245284225350453 20.717628501646036 76-77 20.021368320095696 29.54755283320984 30.066130162922562 20.364948683771907 78-79 19.873054410449747 29.411180530158994 29.735928920681665 20.979836138709597 80-81 19.91835361989562 29.45795627349866 30.289908824338823 20.333781282266898 82-83 20.635537575395386 28.71283327199868 30.405108485803634 20.246520666802304 84-85 21.357563080141194 28.860332452193653 29.35649168231779 20.425612785347358 86-87 20.32071548932638 29.638143109341257 29.60433996198899 20.43680143934337 88-89 20.871193201590323 28.97783488202972 29.52084667198066 20.6301252443993 90-91 20.66644621151477 28.50047689473998 30.34272274185335 20.4903541518919 92-93 20.733281282365876 29.089641326343628 29.533587214582766 20.643490176707722 94-95 19.815672959356938 28.986800195986856 29.970723029666267 21.226803814989935 96-97 20.38609168148965 28.954137601842316 29.186630722132318 21.473139994535718 98-99 19.818436051298114 29.446392624427943 29.468009503274185 21.26716182099976 100-101 20.300079750718183 28.570513276946947 29.407176138014467 21.7222308343204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 91.0 1 64.0 2 57.0 3 74.0 4 64.0 5 69.5 6 99.5 7 157.0 8 249.5 9 305.0 10 313.5 11 371.0 12 538.0 13 766.5 14 944.5 15 1189.5 16 1482.5 17 1654.0 18 1798.0 19 2018.0 20 2319.0 21 2634.0 22 3050.0 23 3630.0 24 4381.5 25 5678.0 26 8453.0 27 14244.0 28 26862.0 29 52314.0 30 86889.5 31 122056.5 32 160630.5 33 199674.0 34 242026.0 35 268913.0 36 274354.0 37 279533.5 38 254709.5 39 198456.5 40 152032.5 41 120145.0 42 99536.0 43 88718.0 44 81925.5 45 81936.5 46 84919.0 47 86527.5 48 87487.0 49 85113.0 50 80470.5 51 78025.5 52 74458.5 53 70405.5 54 66663.5 55 60343.0 56 53779.0 57 45467.5 58 37677.5 59 34188.5 60 29166.5 61 22920.0 62 18018.0 63 14552.0 64 11624.0 65 8432.5 66 6309.5 67 5047.0 68 4047.0 69 3247.5 70 2689.5 71 2175.0 72 1814.5 73 1517.5 74 1246.5 75 1063.0 76 871.0 77 678.0 78 575.5 79 489.0 80 377.5 81 293.5 82 251.0 83 213.0 84 175.0 85 144.5 86 112.5 87 81.5 88 65.0 89 63.5 90 53.5 91 42.5 92 36.5 93 27.5 94 18.5 95 14.0 96 12.5 97 8.5 98 5.0 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.087282505954664E-4 2 0.0038663347045255446 3 1.271820626488666E-4 4 9.665836761313861E-4 5 0.002212967890090279 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.8154618794659983E-5 12-13 1.1446385638397996E-4 14-15 0.003472070310314058 16-17 0.004883791205716478 18-19 0.004107980623558391 20-21 0.0 22-23 0.0 24-25 1.271820626488666E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.0349130023818656E-4 42-43 0.0 44-45 6.995013445687664E-4 46-47 0.7560083350036577 48-49 0.5430165346856008 50-51 0.41755142988249394 52-53 0.3498142633157076 54-55 0.34051725453607545 56-57 0.30615266120835166 58-59 0.30696662640930444 60-61 0.2825095157619274 62-63 0.2781344528068064 64-65 0.2743953001649297 66-67 0.2613337023308911 68-69 0.24064118073792048 70-71 0.2534102598278667 72-73 0.8375956281929057 74-75 0.5513342415828367 76-77 0.6509177966368993 78-79 0.36474543747068455 80-81 0.3153733607503945 82-83 0.29555839538970113 84-85 0.2864012868789827 86-87 0.2579125048456366 88-89 0.26144816618727507 90-91 0.27205515021219057 92-93 0.22726162774725972 94-95 0.1943214735212033 96-97 0.14955338746880223 98-99 0.15766760306579994 100-101 0.15301273957285141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3931372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.436329027892455 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81439272476285 37.52723730756406 2 10.778867623181863 10.010620869963402 3 3.553596389116446 4.950479134720255 4 1.6378452240246224 3.0422207887827835 5 0.866587420383301 2.012056929217576 6 0.5151057274182017 1.43517714255261 7 0.31933005245663565 1.0379960769059342 8 0.22835762989933966 0.8483272030428344 9 0.1543256120166282 0.6449683407331519 >10 0.8897061336685164 7.799998729206095 >50 0.10811421727131276 3.55446868583513 >100 0.11057539463199378 10.560563969931385 >500 0.013136986802057401 4.300454994031641 >1k 0.009179400815283961 8.45016280897214 >5k 5.496647194780814E-4 1.8850033335308838 >10k+ 3.2979883168684885E-4 1.9402636850101376 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 18468 0.4697596665998537 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 13835 0.3519127673494139 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 12447 0.31660702675808855 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 10470 0.2663192391867267 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 10445 0.26568332887348234 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 10351 0.26329230609568366 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9808 0.24948033409201675 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 9394 0.2389496593046906 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 8312 0.21142746094747586 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 7845 0.19954865629607169 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 7638 0.19428331890240863 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 7426 0.18889079944609669 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6711 0.17070376448730876 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5861 0.14908281383700142 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5580 0.14193518191613513 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5276 0.13420251250708404 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4965 0.12629178821032455 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4602 0.11705837046201682 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 4511 0.11474365692180745 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 4450 0.11319203575749129 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4441 0.11296310804472331 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 4384 0.11151323253052624 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 4132 0.10510325657302336 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 4128 0.10500151092290427 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 4043 0.10283941585787354 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3987 0.10141497675620623 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.543641252977332E-5 0.0 7 0.0 0.0 0.0 5.087282505954664E-5 0.0 8 0.0 0.0 0.0 5.087282505954664E-5 0.0 9 0.0 0.0 0.0 5.087282505954664E-5 0.0 10-11 0.0 0.0 0.0 5.087282505954664E-5 0.0 12-13 0.0 0.0 0.0 6.35910313244333E-5 1.271820626488666E-5 14-15 0.0 0.0 0.0 7.630923758931997E-5 2.543641252977332E-5 16-17 0.0 0.0 0.0 7.630923758931997E-5 2.543641252977332E-5 18-19 0.0 0.0 0.0 8.902744385420663E-5 2.543641252977332E-5 20-21 0.0 0.0 0.0 1.0174565011909328E-4 5.087282505954664E-5 22-23 0.0 0.0 0.0 1.271820626488666E-4 5.087282505954664E-5 24-25 0.0 0.0 0.0 2.4164591903284654E-4 5.087282505954664E-5 26-27 0.0 0.0 0.0 5.596010756550131E-4 5.087282505954664E-5 28-29 0.0 0.0 0.0 0.0015134665455215125 5.087282505954664E-5 30-31 0.0 0.0 0.0 0.0059902751507616165 5.087282505954664E-5 32-33 0.0 0.0 0.0 0.0217354145066913 5.087282505954664E-5 34-35 0.0 0.0 0.0 0.04770599169958986 5.087282505954664E-5 36-37 0.0 0.0 0.0 0.088760361522644 5.087282505954664E-5 38-39 0.0 0.0 0.0 0.15067258962011226 5.087282505954664E-5 40-41 0.0 0.0 0.0 0.21617135188427858 5.087282505954664E-5 42-43 0.0 0.0 0.0 0.2889958009570196 5.087282505954664E-5 44-45 0.0 1.271820626488666E-5 0.0 0.3841279838183718 5.087282505954664E-5 46-47 0.0 2.543641252977332E-5 0.0 0.4996601695286022 5.087282505954664E-5 48-49 0.0 2.543641252977332E-5 0.0 0.6206357475202041 5.087282505954664E-5 50-51 0.0 2.543641252977332E-5 0.0 0.7461262887358409 5.087282505954664E-5 52-53 0.0 2.543641252977332E-5 0.0 0.8758138380188901 5.087282505954664E-5 54-55 0.0 5.087282505954664E-5 0.0 1.0259013901508176 5.087282505954664E-5 56-57 0.0 5.087282505954664E-5 0.0 1.2093615155218078 5.087282505954664E-5 58-59 0.0 5.087282505954664E-5 0.0 1.4162740132452487 5.087282505954664E-5 60-61 0.0 6.35910313244333E-5 0.0 1.6314151904220715 5.087282505954664E-5 62-63 0.0 7.630923758931997E-5 0.0 1.8430970154948452 5.087282505954664E-5 64-65 0.0 7.630923758931997E-5 0.0 2.0743140053904847 5.087282505954664E-5 66-67 0.0 7.630923758931997E-5 0.0 2.3467888564094164 5.087282505954664E-5 68-69 0.0 7.630923758931997E-5 0.0 2.653958974118959 5.087282505954664E-5 70-71 0.0 8.902744385420663E-5 0.0 2.971201911190292 5.087282505954664E-5 72-73 0.0 1.0174565011909328E-4 0.0 3.313715924109954 5.087282505954664E-5 74-75 0.0 1.0174565011909328E-4 0.0 3.651168090936192 5.087282505954664E-5 76-77 0.0 1.0174565011909328E-4 0.0 4.0351307380731205 5.087282505954664E-5 78-79 0.0 1.0174565011909328E-4 0.0 4.455556482571479 5.087282505954664E-5 80-81 0.0 1.0174565011909328E-4 0.0 4.938568520099345 5.087282505954664E-5 82-83 0.0 1.0174565011909328E-4 0.0 5.449051883159365 5.087282505954664E-5 84-85 0.0 1.0174565011909328E-4 0.0 5.941717039242279 5.087282505954664E-5 86-87 0.0 1.0174565011909328E-4 0.0 6.4824315785939355 6.35910313244333E-5 88-89 0.0 1.271820626488666E-4 0.0 7.072098493859141 7.630923758931997E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4630 0.0 57.062443 2 ACCTGGG 4835 0.0 54.93543 3 GTACCTG 5380 0.0 49.283657 1 CCTGGGG 3995 0.0 45.74637 4 CTGGGGG 3505 0.0 42.685974 5 GTACCCG 400 0.0 41.429073 1 TGGGGGG 5965 0.0 31.828379 6 GGGGGGA 5920 0.0 28.631357 7 GGGGAAT 6085 0.0 26.9998 9 GTGATCG 825 0.0 25.251043 8 GTATCAA 7740 0.0 24.652515 1 CATGGGG 4785 0.0 24.2421 4 GTACACG 490 0.0 24.156895 1 GGGGGAA 6885 0.0 24.06826 8 GCATCGT 1320 0.0 24.031532 8 TATATCC 6150 0.0 24.01959 4 GAACCGT 435 0.0 23.944956 6 ATGGGGG 4050 0.0 23.380894 5 ATCAACG 7815 0.0 23.264214 3 CAACGCA 7775 0.0 23.26211 5 >>END_MODULE