FastQCFastQC Report
Sat 18 Jun 2016
SRR1931094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931372
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA192910.49069383411185713No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC150920.38388633789933896No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC114730.2918319609540893No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC112290.2856254762968246No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC111690.2840992915450382No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC111560.28376861818215116No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA107600.27369579882036094No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG91690.23322646648549156No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA82930.21094416910941013No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA79340.20181249701122153No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA77680.19759005253127915No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC75140.19112920374871675No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG71680.18232820501341518No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC57570.146437426933905No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT56860.1446314416442911No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT54040.13745837331089503No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC49010.12466385780841906No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA48200.12260350839350741No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA47230.12013617637811938No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA46810.11906784705186893No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC45700.11624440526106408No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG44790.11392969172085471No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA42320.1076468978260007No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT40870.10395861800918356No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT40440.10286485227040332No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA39370.10014315612971757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG47300.059.3475763
TACCTGG50900.055.8966372
CCTGGGG37450.052.6347924
GTACCTG56750.051.185191
CTGGGGG30650.050.520055
GTACCCG3900.041.4460641
TGGGGGG54100.036.2601366
GGGGGGA55850.031.466997
GTACACG4900.031.0472281
TACCGGG2053.092282E-1127.8037932
GGGGAAT65700.026.5324489
ATAACGC3050.026.4743753
GTATCAA74200.025.8208261
TATTCGC3900.025.5759229
GGGGGAA69300.025.08568
GTACGTG3550.024.1053331
CGCCTAC6200.023.749077
TCTAACG6250.023.5590782
CAACGCA76700.023.408475
TAACGCC6100.023.3597434
Produced by FastQC (version 0.11.5)