##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931093_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4791408 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6450122803151 33.0 33.0 33.0 33.0 33.0 2 32.62145490427866 33.0 33.0 33.0 33.0 33.0 3 32.660603939384835 33.0 33.0 33.0 33.0 33.0 4 36.612072902161536 37.0 37.0 37.0 37.0 37.0 5 36.559027742993294 37.0 37.0 37.0 37.0 37.0 6 36.55825949282549 37.0 37.0 37.0 37.0 37.0 7 36.50253829354545 37.0 37.0 37.0 37.0 37.0 8 36.517253592263486 37.0 37.0 37.0 37.0 37.0 9 36.50929956288423 37.0 37.0 37.0 37.0 37.0 10-11 36.48959397738619 37.0 37.0 37.0 37.0 37.0 12-13 36.42994293118015 37.0 37.0 37.0 37.0 37.0 14-15 38.45884226515463 40.0 37.0 40.0 37.0 40.0 16-17 38.467481166287655 40.0 37.0 40.0 37.0 40.0 18-19 38.429018359530225 40.0 37.0 40.0 37.0 40.0 20-21 38.364450804439954 40.0 37.0 40.0 37.0 40.0 22-23 38.27102294356899 40.0 37.0 40.0 37.0 40.0 24-25 38.40226620233551 40.0 37.0 40.0 37.0 40.0 26-27 38.33234667137509 40.0 37.0 40.0 37.0 40.0 28-29 38.29275538630816 40.0 37.0 40.0 37.0 40.0 30-31 38.20510150669699 40.0 37.0 40.0 37.0 40.0 32-33 38.10285035213032 40.0 37.0 40.0 35.0 40.0 34-35 38.05872230041775 40.0 37.0 40.0 35.0 40.0 36-37 38.033057193209174 40.0 37.0 40.0 35.0 40.0 38-39 37.94236339297342 40.0 37.0 40.0 33.0 40.0 40-41 37.81460000901614 40.0 37.0 40.0 33.0 40.0 42-43 37.73005794956305 40.0 37.0 40.0 33.0 40.0 44-45 37.61574290897373 40.0 37.0 40.0 33.0 40.0 46-47 37.486219708277815 40.0 37.0 40.0 33.0 40.0 48-49 37.3709179013768 40.0 37.0 40.0 33.0 40.0 50-51 37.247989004484694 40.0 37.0 40.0 33.0 40.0 52-53 37.09995329139159 40.0 37.0 40.0 33.0 40.0 54-55 36.95850019451485 40.0 37.0 40.0 33.0 40.0 56-57 36.74956766361788 37.0 37.0 40.0 33.0 40.0 58-59 36.56193743050059 37.0 37.0 40.0 33.0 40.0 60-61 36.381619452987515 37.0 37.0 40.0 33.0 40.0 62-63 36.15138650267312 37.0 37.0 40.0 33.0 40.0 64-65 35.86218278635424 37.0 37.0 40.0 33.0 40.0 66-67 35.589482256572595 37.0 35.0 40.0 27.0 40.0 68-69 35.37651103809152 37.0 33.0 40.0 27.0 40.0 70-71 35.13263564697475 37.0 33.0 40.0 27.0 40.0 72-73 34.78764780623983 37.0 33.0 38.5 27.0 40.0 74-75 34.37759579230155 37.0 33.0 37.0 27.0 40.0 76-77 32.734453943392005 35.0 33.0 37.0 27.0 37.0 78-79 33.43021383276064 37.0 33.0 37.0 27.0 37.0 80-81 33.394415691587945 37.0 33.0 37.0 27.0 37.0 82-83 33.135539177627955 37.0 33.0 37.0 27.0 37.0 84-85 32.791659257571055 37.0 33.0 37.0 27.0 37.0 86-87 32.42445842641662 35.0 33.0 37.0 27.0 37.0 88-89 32.057213766809255 33.0 33.0 37.0 27.0 37.0 90-91 31.655609791526835 33.0 33.0 37.0 22.0 37.0 92-93 31.28387521997709 33.0 33.0 37.0 22.0 37.0 94-95 30.851350479858947 33.0 33.0 37.0 18.5 37.0 96-97 30.388039173453816 33.0 33.0 37.0 15.0 37.0 98-99 29.78393240567282 33.0 33.0 37.0 2.0 37.0 100-101 28.021159124833453 33.0 27.5 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.058026393727601544 1101 2 0.19174515049981267 1101 3 0.09453944406482861 1101 4 0.11119489319622033 1101 5 0.1491944363023734 1101 6 0.08398888204593646 1101 7 0.03285699748965243 1101 8 0.1103552368659777 1101 9 0.06348017692778285 1101 10-11 0.10719012864411326 1101 12-13 0.15238199649532902 1101 14-15 0.15978157251922198 1101 16-17 0.22041443919967207 1101 18-19 0.21228761380920247 1101 20-21 0.16553172409865624 1101 22-23 0.1780985986988597 1101 24-25 0.226857228768921 1101 26-27 0.2076001392011193 1101 28-29 0.18025525167642087 1101 30-31 0.11818764085095523 1101 32-33 0.1262155626363466 1101 34-35 0.14690325031031648 1101 36-37 0.028125688186143805 1101 38-39 0.10111608599632405 1101 40-41 0.05969871215506117 1101 42-43 -0.05952498883760171 1101 44-45 -0.02933579169370404 1101 46-47 -0.11632667954410181 1101 48-49 -0.062413383572028636 1101 50-51 -0.02273878726481371 1101 52-53 -0.05567075460806592 1101 54-55 -0.1916212079994466 1101 56-57 -0.16297830476459296 1101 58-59 -0.22407922023266735 1101 60-61 -0.2674262179650242 1101 62-63 -0.3203665316739688 1101 64-65 -0.32381735678297474 1101 66-67 -0.41793893055561426 1101 68-69 -0.4260157008734282 1101 70-71 -0.43067342236881956 1101 72-73 -0.4462331643579276 1101 74-75 -0.5660119611305063 1101 76-77 -0.5162524469898457 1101 78-79 -0.4884342939778463 1101 80-81 -0.7183219083657733 1101 82-83 -0.7978989851881906 1101 84-85 -0.8854306196780186 1101 86-87 -1.1114562582880936 1101 88-89 -1.3274195485622222 1101 90-91 -1.4049542835187445 1101 92-93 -1.3949449064059358 1101 94-95 -1.527364916024542 1101 96-97 -1.7419938580501686 1101 98-99 -1.9530812809676412 1101 100-101 -3.2710923542986343 1102 1 0.10878936021131835 1102 2 0.19679906695341742 1102 3 0.1471304594934466 1102 4 0.16772409021958623 1102 5 0.13275348563033162 1102 6 0.16646539491407708 1102 7 0.1646893887030032 1102 8 0.16104732163162794 1102 9 0.13760696772497027 1102 10-11 0.14189297410936064 1102 12-13 0.11173832499773795 1102 14-15 0.11706925943229152 1102 16-17 0.13142838136163704 1102 18-19 0.17321854887227772 1102 20-21 0.10193072966756489 1102 22-23 0.13621552400424264 1102 24-25 0.19810001064266203 1102 26-27 0.11398836930570866 1102 28-29 0.20804238383040996 1102 30-31 0.17369118996019495 1102 32-33 0.22113967118495736 1102 34-35 0.23869682365310751 1102 36-37 0.13392683782433323 1102 38-39 0.16141095061307453 1102 40-41 0.14913209635634672 1102 42-43 0.12760188649588144 1102 44-45 -5.756317263703181E-4 1102 46-47 -0.10612942576940299 1102 48-49 -0.10075700715092495 1102 50-51 -0.08102859291403064 1102 52-53 -0.07557699979808064 1102 54-55 -0.10616591451999113 1102 56-57 -0.11768410983297883 1102 58-59 -0.11312926588948358 1102 60-61 -0.18075336307089174 1102 62-63 -0.2704528136525468 1102 64-65 -0.2476527027149018 1102 66-67 -0.22596331830713723 1102 68-69 -0.30745378824599356 1102 70-71 -0.27130525989438325 1102 72-73 -0.4758586974070411 1102 74-75 -0.5813803609504689 1102 76-77 -0.4971945899123682 1102 78-79 -0.2713717988936324 1102 80-81 -0.37882756422175845 1102 82-83 -0.443265843594439 1102 84-85 -0.5290179972703655 1102 86-87 -0.5829522605203366 1102 88-89 -0.5730958608967143 1102 90-91 -0.7676602533302379 1102 92-93 -0.7666510782053493 1102 94-95 -0.8432776286023973 1102 96-97 -1.0142054381468704 1102 98-99 -1.0479745279381412 1102 100-101 -1.9444091729872532 1103 1 0.08024846520169149 1103 2 0.1864446163338016 1103 3 0.08133466685870872 1103 4 0.13714421825588374 1103 5 0.07779613774125238 1103 6 0.0888946916359572 1103 7 0.10265853762566479 1103 8 0.08306513078481004 1103 9 0.07853546407216072 1103 10-11 0.07073550153985764 1103 12-13 0.09162981404947601 1103 14-15 0.13123950904562776 1103 16-17 0.08206344881356387 1103 18-19 0.09272721975663956 1103 20-21 0.052236072264385314 1103 22-23 0.12335496907812882 1103 24-25 0.12024208369359712 1103 26-27 0.12279673329192065 1103 28-29 0.09581159206445733 1103 30-31 0.13850864287476838 1103 32-33 0.10039655816373738 1103 34-35 0.13457910426921416 1103 36-37 0.08004142996441033 1103 38-39 0.13186252299490064 1103 40-41 0.042298157918942536 1103 42-43 0.021642371997735665 1103 44-45 0.04491487950502204 1103 46-47 0.12558569318995438 1103 48-49 0.15907535768850067 1103 50-51 0.08046186833411895 1103 52-53 0.008083200123579104 1103 54-55 -0.029400059739927542 1103 56-57 0.12530230773474926 1103 58-59 0.12313589665864555 1103 60-61 0.06756634888574808 1103 62-63 0.0056187574159380915 1103 64-65 -0.002626768250642897 1103 66-67 0.1907921088986697 1103 68-69 0.07641001625945876 1103 70-71 0.12864857245512695 1103 72-73 0.11130212938780204 1103 74-75 0.0024260091054131294 1103 76-77 -0.07457711039148052 1103 78-79 0.04725600473824443 1103 80-81 -0.09492052832428044 1103 82-83 0.015604914598405628 1103 84-85 -0.04700270887708058 1103 86-87 -0.1035321412259691 1103 88-89 -0.26565628187081103 1103 90-91 -0.3019580918583422 1103 92-93 -0.33658293284029384 1103 94-95 -0.3341884945430671 1103 96-97 -0.48268848320836 1103 98-99 -0.4954608310123625 1103 100-101 -1.26891628138101 1104 1 0.06190654000032936 1104 2 0.1648737327551686 1104 3 0.11104025442692489 1104 4 0.11905424257651731 1104 5 0.12077634256548464 1104 6 0.08118312936013439 1104 7 0.10755598821113921 1104 8 0.11568151001131355 1104 9 0.09709101005082488 1104 10-11 0.09551279437108207 1104 12-13 0.12152046652675352 1104 14-15 0.1640103473925265 1104 16-17 0.17592384656295224 1104 18-19 0.1510645464620879 1104 20-21 0.1798936272413485 1104 22-23 0.16447725474512964 1104 24-25 0.1106442132462746 1104 26-27 0.18585986550168343 1104 28-29 0.13629671002355792 1104 30-31 0.1377490758484754 1104 32-33 0.1373394136285242 1104 34-35 0.15181628921551749 1104 36-37 0.17467052403687688 1104 38-39 0.15535906844993264 1104 40-41 0.14373446565185333 1104 42-43 0.10663598196671842 1104 44-45 0.13628510066379107 1104 46-47 0.12141160231535508 1104 48-49 0.09681674773667481 1104 50-51 0.10837649286688844 1104 52-53 0.08451908357063331 1104 54-55 0.022224874042876763 1104 56-57 0.133417060754617 1104 58-59 0.05695466862854914 1104 60-61 0.09681654424793606 1104 62-63 0.06138960758563172 1104 64-65 0.10829618982838696 1104 66-67 0.17066789706923657 1104 68-69 -0.07602394960621695 1104 70-71 0.0022186337773106857 1104 72-73 0.06316763801787317 1104 74-75 0.01048283995513799 1104 76-77 0.011163886262671951 1104 78-79 0.20132139739049393 1104 80-81 0.0758360076801452 1104 82-83 0.16279451660111022 1104 84-85 0.20085429000891253 1104 86-87 0.15439175466782729 1104 88-89 0.036678281923286704 1104 90-91 0.08515014451216629 1104 92-93 0.1799101309917468 1104 94-95 0.07144589766792109 1104 96-97 0.09265162242642688 1104 98-99 0.06743835523597141 1104 100-101 -0.5734829224824658 1105 1 0.03170014394631693 1105 2 0.12976012395755987 1105 3 0.09567137237907275 1105 4 0.13678540211400048 1105 5 0.10238422182942486 1105 6 0.1090676781016029 1105 7 0.1494048313305001 1105 8 0.08442176345077002 1105 9 0.03670998451050167 1105 10-11 0.03748845107266874 1105 12-13 0.10071444762852622 1105 14-15 0.10131595765717094 1105 16-17 0.15790408053816662 1105 18-19 0.16864030250639672 1105 20-21 0.07223023156853259 1105 22-23 0.10604788770843498 1105 24-25 0.18557613171593346 1105 26-27 0.20353164321656436 1105 28-29 0.2269980219828014 1105 30-31 0.15476241925084366 1105 32-33 0.19360071241908372 1105 34-35 0.11736977833979978 1105 36-37 0.09045139680888781 1105 38-39 0.12417476748769474 1105 40-41 0.19174566192302933 1105 42-43 0.245038044194537 1105 44-45 0.16006237659397016 1105 46-47 0.16167536590278786 1105 48-49 0.12738430800651912 1105 50-51 0.13217705224726473 1105 52-53 0.09905210780564033 1105 54-55 0.17799404372254912 1105 56-57 0.19355117342774264 1105 58-59 0.16360532446378073 1105 60-61 0.1425741191304013 1105 62-63 0.00286403838649818 1105 64-65 0.1538373549683314 1105 66-67 0.15290912227687414 1105 68-69 0.08104223459366011 1105 70-71 8.341505004949568E-5 1105 72-73 0.0612486485412731 1105 74-75 0.10339120640270494 1105 76-77 -0.06360324313700261 1105 78-79 0.22373650123829236 1105 80-81 0.07966929384493682 1105 82-83 0.1520547093375484 1105 84-85 0.25881311476141633 1105 86-87 0.18556215775669216 1105 88-89 0.2727657887097763 1105 90-91 0.17573449774484828 1105 92-93 0.21198660322776774 1105 94-95 0.21120922951881482 1105 96-97 0.3945154174916361 1105 98-99 0.26937283091433173 1105 100-101 -0.5497733310005266 1106 1 0.058621957821344495 1106 2 0.15193899916841502 1106 3 0.11681827867057137 1106 4 0.1397755234218252 1106 5 0.14952370788620328 1106 6 0.11400442217079387 1106 7 0.13280100187925825 1106 8 0.08026750870433119 1106 9 0.13027779845731402 1106 10-11 0.14048904774208637 1106 12-13 0.12587967570878078 1106 14-15 0.2914069160966761 1106 16-17 0.2150721663508932 1106 18-19 0.19635979161380845 1106 20-21 0.25756714790919943 1106 22-23 0.22131511036931073 1106 24-25 0.21255586290065764 1106 26-27 0.2510621720540911 1106 28-29 0.26834844638074884 1106 30-31 0.26716392393447563 1106 32-33 0.25236500108146487 1106 34-35 0.3033153872117822 1106 36-37 0.31347527704431855 1106 38-39 0.3265479415895527 1106 40-41 0.3966021472261261 1106 42-43 0.34533442739088827 1106 44-45 0.37538045074248316 1106 46-47 0.3720495628665006 1106 48-49 0.36130987686127725 1106 50-51 0.33139162443978165 1106 52-53 0.3904850789501708 1106 54-55 0.46532576500166556 1106 56-57 0.4943634513590496 1106 58-59 0.45790971110556455 1106 60-61 0.49683819693967735 1106 62-63 0.475964745417329 1106 64-65 0.4957441175208217 1106 66-67 0.5746114021821214 1106 68-69 0.5816686926204397 1106 70-71 0.5998837326755719 1106 72-73 0.6407834258445106 1106 74-75 0.555589170548707 1106 76-77 0.5067980731247061 1106 78-79 0.7146175307397797 1106 80-81 0.6592637829523582 1106 82-83 0.743918224572802 1106 84-85 0.8663763706922154 1106 86-87 0.7982210826750418 1106 88-89 0.6932958599512062 1106 90-91 0.8141991058272531 1106 92-93 0.9282922998505363 1106 94-95 1.1103818554026041 1106 96-97 1.0723525805752914 1106 98-99 1.3130582066814789 1106 100-101 1.0455968956441275 1107 1 0.04171033267743951 1107 2 0.17351416408853737 1107 3 0.09341728498930735 1107 4 0.06571334303191634 1107 5 0.04619888871112465 1107 6 0.07192251561728114 1107 7 0.06426539145623877 1107 8 0.08475455787964847 1107 9 0.03647336007050228 1107 10-11 0.038594881011349 1107 12-13 0.059721948557182714 1107 14-15 0.06231623873130587 1107 16-17 0.07739957558558075 1107 18-19 0.049506403312371106 1107 20-21 0.06628082888000364 1107 22-23 0.10279444624389811 1107 24-25 0.08033290922153924 1107 26-27 0.1061402582960227 1107 28-29 0.10189071891316104 1107 30-31 0.09438491321295572 1107 32-33 0.010831652089102306 1107 34-35 0.079987660934421 1107 36-37 0.09130868533735992 1107 38-39 0.0739689521446465 1107 40-41 0.13180412237039718 1107 42-43 0.21658451297134462 1107 44-45 0.2667037405289747 1107 46-47 0.22060059357118433 1107 48-49 0.16704127114334 1107 50-51 0.1748788200235154 1107 52-53 0.1825738635391545 1107 54-55 0.2660482852486865 1107 56-57 0.28634389372311375 1107 58-59 0.32261833203311596 1107 60-61 0.37563332943929595 1107 62-63 0.2587249547708268 1107 64-65 0.4194078669033203 1107 66-67 0.42124964384870367 1107 68-69 0.43161340501217893 1107 70-71 0.3772883433819274 1107 72-73 0.39656869631561875 1107 74-75 0.3527280341876988 1107 76-77 0.2825149634943003 1107 78-79 0.5147676046286307 1107 80-81 0.5079274322715506 1107 82-83 0.5456699432916992 1107 84-85 0.5416865616656708 1107 86-87 0.5244046998897147 1107 88-89 0.5335545288564845 1107 90-91 0.6093651635121624 1107 92-93 0.8648988477824311 1107 94-95 0.9128802780889558 1107 96-97 1.0212092362069072 1107 98-99 1.1726297672160904 1107 100-101 1.7205847774514602 1108 1 0.04268635769615514 1108 2 0.06053493092134943 1108 3 0.06402972125231088 1108 4 0.10602419120244377 1108 5 0.06650622188945476 1108 6 0.04308441356140946 1108 7 0.059861655423190996 1108 8 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-0.36757891568404943 2316 58-59 -0.4074930712403386 2316 60-61 -0.34682267168437164 2316 62-63 -0.5116568521775733 2316 64-65 -0.609550536041489 2316 66-67 -0.35590193107415047 2316 68-69 -0.49146627500922335 2316 70-71 -0.4894868401127468 2316 72-73 -0.4429018847183883 2316 74-75 -0.4345795376748569 2316 76-77 -0.42088554352565666 2316 78-79 -0.2786149524498214 2316 80-81 -0.2148093305996852 2316 82-83 -0.08693277732601956 2316 84-85 -0.042908686834600474 2316 86-87 -0.154692982788589 2316 88-89 -0.25865336890877444 2316 90-91 -0.10513669551842142 2316 92-93 -0.10189734059236955 2316 94-95 -0.044539265227715674 2316 96-97 0.08433282947115117 2316 98-99 0.21948967310323653 2316 100-101 0.5444648955138227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 12.0 5 28.0 6 76.0 7 188.0 8 479.0 9 1150.0 10 1923.0 11 2847.0 12 4016.0 13 5299.0 14 6702.0 15 7833.0 16 9249.0 17 10998.0 18 11703.0 19 11791.0 20 12778.0 21 14686.0 22 16470.0 23 19708.0 24 23132.0 25 27616.0 26 33737.0 27 41878.0 28 52604.0 29 66422.0 30 85347.0 31 109818.0 32 144115.0 33 195174.0 34 278491.0 35 423734.0 36 702186.0 37 1419721.0 38 1049453.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.32067441975599 26.968998574731152 15.067847016826445 23.642479988686418 2 11.873995988231421 22.899347017981995 45.007506152164616 20.219150841621964 3 15.043761666716756 32.66862266790889 31.656310629359886 20.631305036014467 4 9.565392886600348 20.599477230909997 44.807893629596975 25.027236252892678 5 10.759718228963177 39.92759539575841 38.033434013550924 11.279252361727492 6 23.394605510530518 44.68532005623399 21.32116488514441 10.598909548091083 7 22.065017214146657 33.23492802115787 29.59760471243526 15.102450052260213 8 21.104047077602242 40.32079923062282 22.456697488504425 16.11845620327052 9 24.72131365143607 15.94364328815246 25.16204005169253 34.173003008718936 10-11 21.072250578535577 29.14506550057937 32.2848732564624 17.49781066442265 12-13 21.516295418799654 26.46206501304001 31.588188691090384 20.433450877069955 14-15 19.67623207854915 28.27766235094372 28.171305016597465 23.874800553909665 16-17 18.32197341574752 30.05534072656722 30.837167279430176 20.785518578255076 18-19 17.91933602815707 31.614464892156963 31.41786297472476 19.048336104961212 20-21 18.340965745350847 29.746110120448936 30.968610896838673 20.944313237361545 22-23 18.657876766077948 31.071743420723095 30.038821573950703 20.231558239248255 24-25 18.605324363944796 30.73040325516007 30.95002554572685 19.714246835168282 26-27 19.19136295635855 29.987270965027403 30.192586396316074 20.628779682297978 28-29 18.82998692659861 31.424562466815598 29.63162394018627 20.11382666639952 30-31 20.452808443781034 30.585905019985777 29.14241492271165 19.818871613521537 32-33 19.94570280802637 30.651595522652215 29.61870498191763 19.783996687403786 34-35 20.528422960432508 29.558346941024432 30.278156232990387 19.63507386555267 36-37 20.543115927510243 30.193744719714953 30.594096766545448 18.669042586229352 38-39 21.24248145663374 29.029260424424702 31.551277198841632 18.176980920099925 40-41 19.952617926357004 29.39451148950585 31.551176427679255 19.101694156457892 42-43 20.639730102122762 29.584647345817405 30.43379709507883 19.341825456981006 44-45 19.47670705562958 30.755500262135886 30.391984986459097 19.37580769577544 46-47 20.806357964089052 29.31069531127385 29.630674323706103 20.252272400931 48-49 20.570133930569988 28.068286088795958 30.76335405483219 20.59822592580186 50-51 20.343226876108233 29.125509662295507 29.137833805845798 21.39342965575046 52-53 18.85499001546101 29.341677853357513 30.400114120943154 21.403218010238326 54-55 19.837905684508602 30.098261304401547 29.211507348153194 20.852325662936657 56-57 20.374115500078474 28.81243884887282 30.114342172488755 20.699103478559955 58-59 19.6366809088268 28.413099030598104 31.56526223606923 20.384957824505864 60-61 19.827386855805223 30.892025893015163 28.70695837215282 20.573628879026792 62-63 20.424152983841076 29.462842655019152 28.01446881584703 22.09853554529274 64-65 18.955200642483376 29.43434372526823 28.733672857748704 22.876782774499688 66-67 19.79369030315831 27.94960106793742 29.521243956744865 22.73546467215941 68-69 18.702661353317705 29.213084113124864 28.871741273231333 23.212513260326094 70-71 19.062911483629566 28.76332792149855 29.67942653335145 22.494334061520433 72-73 19.65779161365511 28.786684415103036 29.776122175360562 21.779401795881295 74-75 19.75051996747092 29.71749103681581 28.53836937825446 21.9936196174588 76-77 19.009790125138075 29.680061235894378 29.790532125103635 21.51961651386391 78-79 19.006100857209052 29.00911684717384 29.321610976951156 22.66317131866595 80-81 19.04880127283167 29.509036032047874 29.948017274144075 21.494145420976384 82-83 19.882934868432177 28.337857967736124 29.791445905068457 21.98776125876324 84-85 20.930140579153427 28.048646224766564 28.80864043508982 22.212572760990184 86-87 19.654856329971473 29.442650951831155 28.642623151999956 22.25986956619741 88-89 20.269459415687415 27.999045374553784 29.147809996560508 22.583685213198294 90-91 20.136796964148843 27.238645429344093 29.991437797766107 22.633119808740958 92-93 19.22082061031461 28.62662776460531 29.214405923509844 22.938145701570235 94-95 18.432118599716045 27.78801114742157 29.682707564895743 24.097162687966637 96-97 18.94182236404975 27.782503390805623 29.08631341761378 24.189360827530848 98-99 18.082847463626557 28.634641424817087 29.634107552519005 23.64840355903734 100-101 17.524281044005583 27.648259628934557 29.323586965958953 25.50387236110091 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17.0 1 13.5 2 14.5 3 15.0 4 9.5 5 7.0 6 13.5 7 25.0 8 47.0 9 62.5 10 66.5 11 84.0 12 108.0 13 142.0 14 181.0 15 224.5 16 268.5 17 328.0 18 399.0 19 481.5 20 551.0 21 627.5 22 756.5 23 880.0 24 1035.0 25 1332.5 26 1954.0 27 3496.0 28 8287.5 29 21165.0 30 40585.5 31 64801.0 32 100332.5 33 151066.5 34 226448.0 35 291030.0 36 345457.5 37 420089.5 38 441356.0 39 372100.5 40 293498.5 41 233602.0 42 191345.0 43 172987.0 44 153602.5 45 138181.5 46 126918.0 47 113022.5 48 100716.5 49 90022.0 50 81881.5 51 78734.5 52 74573.0 53 70406.5 54 67056.5 55 59434.0 56 51131.5 57 41988.0 58 33671.5 59 31372.0 60 26009.5 61 18371.5 62 13227.5 63 10364.0 64 7685.0 65 4322.5 66 2712.0 67 1978.0 68 1539.0 69 1213.0 70 915.5 71 685.0 72 532.5 73 416.0 74 330.0 75 277.0 76 202.0 77 146.5 78 114.0 79 83.5 80 63.5 81 48.5 82 41.0 83 33.0 84 24.0 85 21.0 86 20.0 87 15.0 88 10.0 89 6.0 90 3.0 91 1.0 92 0.5 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0885126042282352 2 1.0435345935891913E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.713189943331897E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.391811342302721E-5 40-41 0.003015814975472763 42-43 0.005478556616343255 44-45 0.0 46-47 0.0 48-49 1.565301890383787E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002421000257126924 68-69 0.006897763663624555 70-71 0.001189629436691678 72-73 0.0 74-75 5.2176729679459565E-5 76-77 0.0020975045331142745 78-79 0.0058020523403559034 80-81 0.004195009066228549 82-83 0.004779388438638496 84-85 0.002535789062421735 86-87 2.504483024614059E-4 88-89 0.0 90-91 1.0435345935891912E-5 92-93 0.0012418061663711378 94-95 0.003454099504780223 96-97 0.0029740735917291954 98-99 0.0 100-101 3.339310699485412E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4791408.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.85938264634758 #Duplication Level Percentage of deduplicated Percentage of total 1 71.70332461496606 17.825003836187147 2 14.32126610028463 7.120356679342832 3 5.366633667509869 4.002335995901985 4 2.573214020673359 2.5587404788346233 5 1.4343120250197632 1.7828055732111978 6 0.8925126687851589 1.3312388370025885 7 0.5836261644370481 1.0156010300912526 8 0.4097990430903595 0.8149880976232262 9 0.308131335963851 0.6893959307450088 >10 1.8372059590995675 8.738793503350749 >50 0.23309334385905847 4.099031922359546 >100 0.26235703467339894 13.928623926409356 >500 0.04063413849502714 6.969732691944394 >1k 0.028494478861581716 13.768097925613255 >5k 0.003203521292130488 5.3223668581278325 >10k+ 0.0021918829893524394 10.032886713255063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 37303 0.778539418893152 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 32701 0.6824924948992029 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 32483 0.677942684071154 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 29885 0.6237206265882597 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 23586 0.4922561384878933 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 23509 0.490649095213766 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 23234 0.48490965494902544 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 21102 0.44041333987838227 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 20512 0.42809963167402987 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 20095 0.419396553163496 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 18395 0.38391637698146347 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 16954 0.35384170999422304 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 16547 0.345347338402407 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 16234 0.33881481184653867 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 15478 0.3230365687914701 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 14239 0.2971777815623299 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 13964 0.29143834129758933 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 12878 0.2687727699248321 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 12545 0.26182282953152813 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 11799 0.24625329339517735 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 11656 0.24326878445751227 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 11327 0.2364023268316954 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 11107 0.23181077461990296 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 10731 0.22396339447611227 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 10313 0.2152394452737066 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 10154 0.21192100526609298 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 9713 0.20271703015063627 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 9078 0.18946414081205357 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 8422 0.1757729669441634 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 8162 0.17034658705749958 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 8126 0.16959524215011537 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 8079 0.16861431963214152 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 7874 0.16433582779842587 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 7571 0.15801200816127534 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 7489 0.1563006114277891 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 7440 0.15527794752607166 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 7404 0.15452660261868745 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 7275 0.15183428336722735 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 7104 0.1482653950571523 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 7009 0.14628267932933284 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 6840 0.14275553240300137 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6746 0.1407936873670537 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 6641 0.13860226472051637 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 6594 0.13762134220254255 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 6573 0.1371830576732351 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 6552 0.13674477314392763 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 6474 0.13511685917792848 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 6157 0.12850084985457302 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 6064 0.12655987551049713 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5969 0.12457715978267767 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 5938 0.12393016833465237 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 5937 0.12390929764278058 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 5913 0.12340840103785776 No Hit ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA 5864 0.12238573713614034 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5848 0.1220518060661918 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 5749 0.11998560757088522 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 5743 0.1198603834196545 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 5717 0.11931774543098812 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 5700 0.1189629436691678 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 5487 0.11451748630047787 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 5312 0.11086511522291567 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5307 0.11076076176355677 No Hit ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC 5075 0.10591876124930293 No Hit CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT 5017 0.10470826112073946 No Hit CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA 4843 0.10107676073504908 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 4837 0.10095153658381836 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 4824 0.10068021758948519 No Hit CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC 4818 0.10055499343825447 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0870691871783828E-5 0.0 12-13 0.0 0.0 0.0 2.0870691871783828E-5 0.0 14-15 0.0 0.0 0.0 3.130603780767574E-5 0.0 16-17 0.0 0.0 0.0 5.2176729679459565E-5 0.0 18-19 0.0 0.0 0.0 8.348276748713531E-5 0.0 20-21 0.0 0.0 0.0 1.1478880529481105E-4 0.0 22-23 0.0 0.0 0.0 3.4436641588443315E-4 0.0 24-25 0.0 0.0 0.0 7.826509451918935E-4 0.0 26-27 0.0 0.0 0.0 0.0021288105709219504 0.0 28-29 0.0 0.0 0.0 0.006876892971752771 0.0 30-31 0.0 2.0870691871783828E-5 0.0 0.03256871466591866 0.0 32-33 0.0 2.0870691871783828E-5 0.0 0.11756460731375828 0.0 34-35 0.0 2.0870691871783828E-5 0.0 0.2506048326504443 0.0 36-37 0.0 2.0870691871783828E-5 0.0 0.46753480396576536 0.0 38-39 0.0 2.0870691871783828E-5 0.0 0.769471103274862 0.0 40-41 0.0 2.0870691871783828E-5 0.0 1.0744232175594315 0.0 42-43 0.0 2.0870691871783828E-5 0.0 1.4275135826462702 0.0 44-45 0.0 3.130603780767574E-5 0.0 1.8061079332004288 0.0 46-47 0.0 4.1741383743567656E-5 0.0 2.258949770088458 0.0 48-49 0.0 4.1741383743567656E-5 0.0 2.709662796405566 0.0 50-51 0.0 4.1741383743567656E-5 0.0 3.14547414872622 0.0 52-53 0.0 4.1741383743567656E-5 0.0 3.5878702043324218 0.0 54-55 0.0 4.1741383743567656E-5 0.0 4.031361971261892 0.0 56-57 0.0 4.1741383743567656E-5 0.0 4.560809682665305 0.0 58-59 0.0 4.1741383743567656E-5 0.0 5.178895222448182 0.0 60-61 0.0 4.1741383743567656E-5 0.0 5.78194342873744 0.0 62-63 0.0 4.1741383743567656E-5 0.0 6.362169533464902 0.0 64-65 0.0 4.1741383743567656E-5 0.0 6.963495907674738 0.0 66-67 0.0 6.261207561535148E-5 0.0 7.59993722095885 0.0 68-69 0.0 6.261207561535148E-5 0.0 8.259409342723474 0.0 70-71 0.0 6.261207561535148E-5 0.0 8.911816735289502 0.0 72-73 0.0 6.261207561535148E-5 0.0 9.636509769153452 0.0 74-75 0.0 6.261207561535148E-5 0.0 10.297870688532473 0.0 76-77 0.0 6.261207561535148E-5 0.0 11.026414365046767 0.0 78-79 0.0 6.261207561535148E-5 0.0 11.814867362579017 0.0 80-81 0.0 6.261207561535148E-5 0.0 12.686876175019954 0.0 82-83 0.0 6.261207561535148E-5 0.0 13.61628982545423 0.0 84-85 0.0 6.261207561535148E-5 0.0 14.461813729909872 0.0 86-87 0.0 6.261207561535148E-5 0.0 15.375991775277747 0.0 88-89 0.0 6.261207561535148E-5 0.0 16.30020862343595 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3175 0.0 46.675026 3 TACCTGG 3315 0.0 45.563522 2 CCTGGGG 2670 0.0 45.0073 4 CTGGGGG 2490 0.0 44.064247 5 GTACCCG 255 0.0 39.148537 1 GAACCGT 760 0.0 34.998398 6 GTACCTG 4355 0.0 34.92997 1 TGAACCG 800 0.0 33.248478 5 AACCGTG 835 0.0 32.9925 7 TGGGGGG 7500 0.0 31.918537 6 CCGTGTT 810 0.0 31.079147 9 ACCGTGT 880 0.0 30.765957 8 TATTCGC 1210 0.0 30.61875 9 CTGTGCG 2060 0.0 29.05237 9 TCTCTGC 4590 0.0 27.836706 8 GGGGGGA 7825 0.0 27.193644 7 GTATCAA 8135 0.0 26.705153 1 TATTCTC 4960 0.0 26.43024 5 CTCCGAC 680 0.0 25.846157 94-95 ATGGGGG 6980 0.0 25.518173 5 >>END_MODULE