##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931092_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3601834 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.043938449134526 33.0 33.0 33.0 27.0 33.0 2 30.948560927571897 33.0 33.0 33.0 27.0 33.0 3 30.98012373696289 33.0 33.0 33.0 27.0 33.0 4 34.55611363544239 37.0 37.0 37.0 33.0 37.0 5 34.54728618809195 37.0 37.0 37.0 33.0 37.0 6 34.65139842646829 37.0 37.0 37.0 33.0 37.0 7 34.61767449582629 37.0 37.0 37.0 33.0 37.0 8 34.617643400556496 37.0 37.0 37.0 33.0 37.0 9 34.62666186170712 37.0 37.0 37.0 33.0 37.0 10-11 34.525417884333365 37.0 37.0 37.0 33.0 37.0 12-13 34.44260659985996 37.0 37.0 37.0 33.0 37.0 14-15 36.02545897451132 40.0 37.0 40.0 33.0 40.0 16-17 35.998811855293724 40.0 37.0 40.0 33.0 40.0 18-19 35.940237390174005 40.0 37.0 40.0 27.0 40.0 20-21 35.84683899924316 40.0 37.0 40.0 27.0 40.0 22-23 35.74817745626256 40.0 37.0 40.0 27.0 40.0 24-25 35.61174654356641 40.0 37.0 40.0 27.0 40.0 26-27 35.41778549483402 38.5 37.0 40.0 27.0 40.0 28-29 35.317747986164825 37.0 37.0 40.0 27.0 40.0 30-31 35.19987262044836 37.0 37.0 40.0 27.0 40.0 32-33 35.033223074689175 37.0 37.0 40.0 27.0 40.0 34-35 34.8797899903216 37.0 37.0 40.0 27.0 40.0 36-37 34.66138708780027 37.0 37.0 40.0 24.5 40.0 38-39 34.47746939475834 37.0 37.0 40.0 22.0 40.0 40-41 34.28705612196453 37.0 33.0 40.0 22.0 40.0 42-43 34.1031691077379 37.0 33.0 40.0 22.0 40.0 44-45 33.82792294147926 37.0 33.0 40.0 22.0 40.0 46-47 33.67947814918733 37.0 33.0 40.0 15.0 40.0 48-49 33.648489075287756 37.0 33.0 40.0 15.0 40.0 50-51 32.81665520954047 37.0 33.0 38.5 15.0 40.0 52-53 32.96795327047276 37.0 33.0 38.5 15.0 40.0 54-55 33.46857600877775 37.0 33.0 40.0 15.0 40.0 56-57 33.38745955532654 37.0 33.0 40.0 15.0 40.0 58-59 33.2259479476289 37.0 33.0 40.0 15.0 40.0 60-61 33.196559724851284 37.0 33.0 40.0 15.0 40.0 62-63 33.06242486466617 37.0 33.0 40.0 15.0 40.0 64-65 32.84480808943444 37.0 33.0 40.0 6.0 40.0 66-67 32.56254397065494 37.0 33.0 40.0 6.0 40.0 68-69 32.30016749800241 37.0 33.0 40.0 6.0 40.0 70-71 32.03017393361271 37.0 33.0 38.5 2.0 40.0 72-73 31.398785313259857 37.0 33.0 37.0 2.0 40.0 74-75 31.22733904449789 37.0 33.0 37.0 2.0 40.0 76-77 30.89765519454811 37.0 33.0 37.0 2.0 40.0 78-79 30.62097226024298 37.0 33.0 37.0 2.0 37.0 80-81 30.3109635258038 37.0 33.0 37.0 2.0 37.0 82-83 30.020572852607867 37.0 30.0 37.0 2.0 37.0 84-85 29.621059715689285 33.0 27.0 37.0 2.0 37.0 86-87 29.49876215838931 33.0 27.0 37.0 2.0 37.0 88-89 29.294714303879637 33.0 27.0 37.0 2.0 37.0 90-91 28.98846837472243 33.0 27.0 37.0 2.0 37.0 92-93 28.682362235461156 33.0 27.0 37.0 2.0 37.0 94-95 28.411835054030806 33.0 27.0 37.0 2.0 37.0 96-97 28.061491034845027 33.0 27.0 37.0 2.0 37.0 98-99 27.766580580892956 33.0 27.0 37.0 2.0 37.0 100-101 27.04927517481372 33.0 24.5 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17213490213384475 1101 2 0.1587772170676729 1101 3 0.11113664971087545 1101 4 0.2138973203718706 1101 5 0.031161833494742552 1101 6 0.0058753464674197176 1101 7 0.15351195044547694 1101 8 0.08678694086452055 1101 9 0.19159950749625665 1101 10-11 0.14159761488013345 1101 12-13 0.14931680152650273 1101 14-15 0.29917328717191083 1101 16-17 0.24600679184083418 1101 18-19 0.350614963533161 1101 20-21 0.3653540031916407 1101 22-23 0.3124579159862577 1101 24-25 0.3359025636736206 1101 26-27 0.2751355153112556 1101 28-29 0.2980296197652521 1101 30-31 0.38406997560671385 1101 32-33 0.21190937470019122 1101 34-35 0.2492823939404687 1101 36-37 0.24238840450386334 1101 38-39 0.16990428470414543 1101 40-41 0.34326817768992157 1101 42-43 0.2302037056377273 1101 44-45 0.18780100491538576 1101 46-47 0.2637190498678592 1101 48-49 0.16668274840237274 1101 50-51 0.15925069321730945 1101 52-53 0.23415805782625654 1101 54-55 0.39746038391166394 1101 56-57 0.33104225417093147 1101 58-59 0.3089779722653603 1101 60-61 0.24907362563766355 1101 62-63 0.33072754506174107 1101 64-65 0.4661291656393516 1101 66-67 0.4538869078054759 1101 68-69 0.31452633892569537 1101 70-71 0.20370440698324188 1101 72-73 0.1150419544482375 1101 74-75 0.17173982963157286 1101 76-77 0.2507602427158808 1101 78-79 0.28402147040033654 1101 80-81 0.34933723808762807 1101 82-83 0.24378361568571094 1101 84-85 0.25229421209650127 1101 86-87 0.23631708079371094 1101 88-89 0.2753564911862192 1101 90-91 0.23437106966474985 1101 92-93 -0.02437784541930199 1101 94-95 -0.013064464512112295 1101 96-97 -0.17440085150843387 1101 98-99 -0.0244866018993406 1101 100-101 0.07687716006378054 1102 1 -0.07050458276658844 1102 2 -0.08419803616898847 1102 3 -0.14581712163179716 1102 4 -0.08292643855920545 1102 5 -0.17034830507112275 1102 6 -0.09040099178323402 1102 7 -0.08203181227094802 1102 8 -0.05311016297434179 1102 9 -0.08560955697490868 1102 10-11 -0.05822503756233033 1102 12-13 -0.05368791560915298 1102 14-15 0.0011870782421112835 1102 16-17 -0.03462729164809275 1102 18-19 -0.039815365901986866 1102 20-21 -0.10114423334213996 1102 22-23 0.08750405000969153 1102 24-25 0.0018285381297502568 1102 26-27 -0.0045053672590000815 1102 28-29 -0.05243972795243934 1102 30-31 0.05493238156427793 1102 32-33 -0.011059909321609496 1102 34-35 -0.07883925065102204 1102 36-37 -0.03351534918594723 1102 38-39 0.07187903929298045 1102 40-41 0.08199355012889953 1102 42-43 0.016943495186502844 1102 44-45 0.0683425473750674 1102 46-47 -0.1390296097886221 1102 48-49 -0.14464092499421355 1102 50-51 -0.23938093706991026 1102 52-53 -0.1672188653346609 1102 54-55 -0.0158499643046639 1102 56-57 -0.05988626812080611 1102 58-59 -0.10821464046727414 1102 60-61 -0.05905842868219224 1102 62-63 0.024531845808247965 1102 64-65 0.06286547921239816 1102 66-67 0.03870208053053403 1102 68-69 0.015640653030153828 1102 70-71 0.012876069227594655 1102 72-73 -0.13565142081519 1102 74-75 -0.1463419777302164 1102 76-77 -0.15594848143723894 1102 78-79 -0.0522232200922339 1102 80-81 -0.08973400303589685 1102 82-83 -0.05104418702445912 1102 84-85 -0.06373931742017191 1102 86-87 -0.03435815024038291 1102 88-89 0.0048500542615279585 1102 90-91 -0.03512760728061082 1102 92-93 -0.07642102759318803 1102 94-95 -0.10422602276837623 1102 96-97 -0.13740354857441517 1102 98-99 -0.14941653641996666 1102 100-101 -0.1339785305270631 1103 1 -0.02499211359695508 1103 2 -0.12270363892164582 1103 3 -0.12522205753509041 1103 4 -0.07902925211543277 1103 5 -0.14299187560794735 1103 6 -0.1004634898801271 1103 7 -0.04137721892726631 1103 8 -0.047449175184688386 1103 9 -0.039590099589318584 1103 10-11 -0.07324951154302539 1103 12-13 -0.06344230037210252 1103 14-15 -0.07548099672515463 1103 16-17 -0.04770088867620359 1103 18-19 -0.02959319696680751 1103 20-21 -0.07853396721138495 1103 22-23 -0.026692000658677273 1103 24-25 -0.021518802085225275 1103 26-27 -0.07822229122599822 1103 28-29 -0.05696551127206817 1103 30-31 -0.0016124100072261172 1103 32-33 -0.09879513358395542 1103 34-35 -0.12999824398253423 1103 36-37 -0.11084970258566784 1103 38-39 -0.14160829361406257 1103 40-41 0.0054413744947794385 1103 42-43 -0.058189151505132486 1103 44-45 -0.08140700349122909 1103 46-47 -0.07187479568430888 1103 48-49 -0.11557398123489548 1103 50-51 -0.037557568414875675 1103 52-53 0.05969680410293421 1103 54-55 0.1459475981934304 1103 56-57 0.1318285472015006 1103 58-59 0.07182003417722882 1103 60-61 0.1684340730686671 1103 62-63 0.16286521720376612 1103 64-65 0.32346846024220355 1103 66-67 0.2571827906185291 1103 68-69 0.12988218800168028 1103 70-71 0.12594643097053648 1103 72-73 -0.02971847876105116 1103 74-75 -0.023504290083675272 1103 76-77 -0.04339310096770177 1103 78-79 0.029792781247557798 1103 80-81 -0.02280775231271548 1103 82-83 0.07223692500014778 1103 84-85 0.2019820750465584 1103 86-87 0.09157170877730891 1103 88-89 0.033586618312565264 1103 90-91 0.019227657479902405 1103 92-93 -0.012814750378730366 1103 94-95 0.013623426091282198 1103 96-97 -0.1122328397044079 1103 98-99 0.08823704446977487 1103 100-101 0.12219648698154018 1104 1 -0.11977118850882817 1104 2 -0.06976921000158143 1104 3 0.0012067126771313497 1104 4 -0.10217625721040946 1104 5 -0.15876864564890525 1104 6 -0.15391562277769566 1104 7 -0.13770351871747266 1104 8 -0.2059174350428279 1104 9 -0.09860585548707945 1104 10-11 -0.16052393033022128 1104 12-13 -0.1528563957637843 1104 14-15 -0.06174159342270258 1104 16-17 -0.10196668382099006 1104 18-19 -0.06712379180903838 1104 20-21 0.045527404436562335 1104 22-23 -0.09041044248031227 1104 24-25 -0.037752800001563 1104 26-27 -0.011158932251262854 1104 28-29 -0.18889894474478552 1104 30-31 -0.09865050816760856 1104 32-33 -0.04386267415112144 1104 34-35 -0.08291411976211549 1104 36-37 -0.03372911430839309 1104 38-39 -0.16629128466573917 1104 40-41 -0.055461806655230816 1104 42-43 -0.07413049147002226 1104 44-45 -0.04581779856465573 1104 46-47 -0.23655006220730002 1104 48-49 -0.1819947593250575 1104 50-51 -0.2505491374396911 1104 52-53 -0.11824190631108422 1104 54-55 -0.05906950994283733 1104 56-57 0.020071475600651922 1104 58-59 -0.06611962173337105 1104 60-61 0.025846320282127522 1104 62-63 0.049758047842139774 1104 64-65 0.03854163810973432 1104 66-67 0.18776193403430597 1104 68-69 0.15686443050550025 1104 70-71 0.12691378101121842 1104 72-73 -0.042393395628710806 1104 74-75 -0.09805954989900201 1104 76-77 -0.11785728983867116 1104 78-79 0.08652239776841597 1104 80-81 0.07959737691704305 1104 82-83 -0.025080719036445487 1104 84-85 0.09076839638695589 1104 86-87 0.11344175829992054 1104 88-89 -0.001545001947466318 1104 90-91 0.09689422543434745 1104 92-93 -0.017608864038123784 1104 94-95 -0.1764216544721613 1104 96-97 -0.07490615101183451 1104 98-99 0.02332915010850556 1104 100-101 0.12225152201843059 1105 1 0.026098708974615192 1105 2 -0.008097366648438964 1105 3 0.03494711461376809 1105 4 0.1647699399707676 1105 5 0.024684712258753905 1105 6 0.007942061314381021 1105 7 0.0417916197747914 1105 8 0.02394985990363807 1105 9 -0.06563941498136217 1105 10-11 0.023502898999453237 1105 12-13 0.08297926842561054 1105 14-15 0.09316610367706346 1105 16-17 0.03500503208850603 1105 18-19 -0.0410078015892168 1105 20-21 0.05631065873772201 1105 22-23 0.07487511245620482 1105 24-25 -0.09108935039680688 1105 26-27 -0.028282120612381334 1105 28-29 0.00416514256152567 1105 30-31 0.05088366504609354 1105 32-33 0.03216566210345917 1105 34-35 0.01581604351807897 1105 36-37 0.04890943420393512 1105 38-39 0.05601459542457121 1105 40-41 0.13994495557444964 1105 42-43 0.09094749047721251 1105 44-45 0.1355699539456836 1105 46-47 0.20556861387866832 1105 48-49 0.220153366959984 1105 50-51 0.20839057539794936 1105 52-53 0.3304051527771321 1105 54-55 0.492082524314128 1105 56-57 0.34050254911882405 1105 58-59 0.32034153628558926 1105 60-61 0.3534265791992297 1105 62-63 0.4661745464199569 1105 64-65 0.5086984169635898 1105 66-67 0.5613783154846317 1105 68-69 0.3824508137840681 1105 70-71 0.4562938669036001 1105 72-73 0.5022037986111094 1105 74-75 0.4706761081073729 1105 76-77 0.4669351096734182 1105 78-79 0.4977038238940139 1105 80-81 0.42005874392558695 1105 82-83 0.3929950301887146 1105 84-85 0.46177190804528934 1105 86-87 0.5335143663482853 1105 88-89 0.5706678994429488 1105 90-91 0.5293186346179546 1105 92-93 0.48600112273086893 1105 94-95 0.45322192662271377 1105 96-97 0.38406056048037485 1105 98-99 0.38023332271723476 1105 100-101 0.595364570218198 1106 1 0.030456078355964422 1106 2 -0.08038500952885386 1106 3 -0.0463138180466629 1106 4 0.030331423567893978 1106 5 0.03209513135260522 1106 6 0.044439882877497894 1106 7 0.014624846603830122 1106 8 -0.012924388811534016 1106 9 -0.027031387575284782 1106 10-11 0.0039910945347330085 1106 12-13 -0.023756869440070716 1106 14-15 0.023270842446372342 1106 16-17 -0.012475095002955072 1106 18-19 -0.027737402161761793 1106 20-21 -0.011353629369722285 1106 22-23 -0.0034158702755675563 1106 24-25 -0.05123556967053844 1106 26-27 0.05672338247668307 1106 28-29 -0.03902262462162298 1106 30-31 0.08961332707612968 1106 32-33 0.12782970372009572 1106 34-35 0.007291666039542122 1106 36-37 0.07508983111361545 1106 38-39 -0.019486597049066745 1106 40-41 0.002671299230222246 1106 42-43 0.040404235965866064 1106 44-45 -0.021624025731057372 1106 46-47 -0.021336130639880935 1106 48-49 -0.05918003069445632 1106 50-51 0.018988821149953594 1106 52-53 0.009447808573533223 1106 54-55 8.538043552164254E-5 1106 56-57 0.03740583484793092 1106 58-59 0.019377826605499138 1106 60-61 -0.025115032811022786 1106 62-63 0.01436944729827161 1106 64-65 0.02616143177748853 1106 66-67 0.1419445471688121 1106 68-69 0.15656821312759206 1106 70-71 0.17350493077247364 1106 72-73 -0.007475467612842124 1106 74-75 0.1254763844678486 1106 76-77 -0.012133905110083276 1106 78-79 0.14939269070372774 1106 80-81 0.25303685184098157 1106 82-83 0.32438451144120606 1106 84-85 0.38758313044806414 1106 86-87 0.4287180219457767 1106 88-89 0.38442344135596684 1106 90-91 0.29536759013792846 1106 92-93 0.3958870067374498 1106 94-95 0.29611769636461105 1106 96-97 0.3902426144294857 1106 98-99 0.3363147565403999 1106 100-101 0.35307723792941914 1107 1 -0.0346412793657187 1107 2 -0.0012572843978588821 1107 3 0.03188319273428064 1107 4 0.07904654701605551 1107 5 0.043437173923507544 1107 6 -0.039656440544575844 1107 7 -0.08525068025223703 1107 8 0.04743809748321581 1107 9 -0.021041923143698682 1107 10-11 -0.04295985698985305 1107 12-13 -0.011670783409947205 1107 14-15 0.076892521738948 1107 16-17 -0.008033275132596884 1107 18-19 0.022737467153085333 1107 20-21 0.06964717197952552 1107 22-23 0.049598119170234156 1107 24-25 0.06985645504084204 1107 26-27 0.10229449135147206 1107 28-29 -0.005215625550320624 1107 30-31 -0.014274032105376477 1107 32-33 0.10691837084024058 1107 34-35 0.0861431508651549 1107 36-37 0.10974119760044942 1107 38-39 -0.029482963023404807 1107 40-41 0.04042766110462992 1107 42-43 0.020048800302099323 1107 44-45 0.0370619944517685 1107 46-47 -0.15591400128720068 1107 48-49 -0.08442703572929844 1107 50-51 -0.08089004466132366 1107 52-53 0.029383273557989753 1107 54-55 0.15104420412936292 1107 56-57 0.21645016374899484 1107 58-59 0.17979808459026003 1107 60-61 0.14358773650288725 1107 62-63 0.09849248236195507 1107 64-65 0.17969948188977725 1107 66-67 0.15956794097605353 1107 68-69 0.20621944760922162 1107 70-71 0.08560518794967464 1107 72-73 -0.05693731344939934 1107 74-75 0.07573208631623274 1107 76-77 0.08676431732334677 1107 78-79 0.13122330007081828 1107 80-81 0.24824638292743373 1107 82-83 0.1651565710640739 1107 84-85 0.1779014027639363 1107 86-87 0.24323649801556257 1107 88-89 0.28452982202170674 1107 90-91 0.3193445886190922 1107 92-93 0.3183626013044254 1107 94-95 0.36248125578403645 1107 96-97 0.3533271645402145 1107 98-99 0.3341615963357789 1107 100-101 0.305301059744135 1108 1 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-0.8272576802968317 2316 60-61 -0.7446323252111497 2316 62-63 -0.8335305906249673 2316 64-65 -0.8117055203586077 2316 66-67 -0.8285048133617607 2316 68-69 -0.8524703954179742 2316 70-71 -0.7546965595809851 2316 72-73 -0.5069587634708483 2316 74-75 -0.5794311664373843 2316 76-77 -0.7325463875295277 2316 78-79 -0.6857191156667817 2316 80-81 -0.6798619319278707 2316 82-83 -0.6190789553401252 2316 84-85 -0.583098323296035 2316 86-87 -0.5427625819594226 2316 88-89 -0.5636851743860589 2316 90-91 -0.6289660283662251 2316 92-93 -0.5603315834138556 2316 94-95 -0.5205964863298469 2316 96-97 -0.5806677598161549 2316 98-99 -0.4621712252982526 2316 100-101 -0.5479864790855729 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112099.0 3 16580.0 4 12729.0 5 10525.0 6 10694.0 7 11440.0 8 11812.0 9 12446.0 10 12448.0 11 12549.0 12 13153.0 13 13958.0 14 14764.0 15 16086.0 16 18947.0 17 22961.0 18 20606.0 19 17086.0 20 17695.0 21 18281.0 22 20277.0 23 23018.0 24 26273.0 25 30603.0 26 34619.0 27 41157.0 28 48979.0 29 59466.0 30 72750.0 31 91156.0 32 117900.0 33 155914.0 34 214625.0 35 308070.0 36 465230.0 37 779174.0 38 715366.0 39 398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18391066279381 22.023930164078433 12.937175545433496 22.854983627694267 2 14.197898830805041 22.20842938727143 41.90643941528869 21.68723236663484 3 19.3819468236389 29.274790064817125 29.601069013328242 21.74219409821574 4 11.357485385052774 17.68589080102349 42.84217411777249 28.11444969615125 5 11.923744010919098 38.9314066472447 36.025885101268464 13.118964240567744 6 26.2059828409638 40.47218722461946 20.180108244855262 13.141721689561484 7 24.711688545335512 32.43095045468503 26.23288580206639 16.624475197913064 8 23.386974524644945 35.32877972721675 21.829878889476863 19.454366858661448 9 25.138720996025913 16.90591515322472 23.844019463417805 34.11134438733156 10-11 23.780211353496956 26.51674855552714 30.013718015243906 19.689322075731997 12-13 25.067455265467025 24.72822507105066 29.17655920328311 21.027760460199207 14-15 21.614453475961962 26.479284272906078 28.02206619748186 23.8841960536501 16-17 20.213532597476462 28.65397998668111 29.157596174976952 21.974891240865475 18-19 19.800852845253857 30.25972257332874 29.63364469945305 20.30577988196435 20-21 21.246606589865053 27.99089297285772 28.668408927229848 22.09409151004738 22-23 20.924104220238913 28.64915484722505 28.379222918102275 22.04751801443376 24-25 21.724921487903316 28.37717234847197 28.845489387557755 21.052416776066966 26-27 21.525936509011796 27.64043262404653 29.365137316156158 21.468493550785517 28-29 20.387724698028837 29.936873825945337 28.47109278217708 21.204308693848745 30-31 21.755583405565055 28.182850736596965 28.126587732805007 21.93497812503297 32-33 21.907256136734784 28.575275817819477 28.230284904856806 21.287183140588933 34-35 22.533756414093485 27.608199045264165 28.83665654774762 21.02138799289473 36-37 22.244945214021524 27.60120260955946 29.261037571415006 20.892814605004006 38-39 22.16161266732448 28.04274155888361 29.46926482453106 20.32638094926085 40-41 21.568820708049095 28.130630389886907 29.122613207493476 21.177935694570518 42-43 21.603427809753004 28.270101880056366 27.92769293749975 22.19877737269088 44-45 21.641634821584248 28.200486310779134 28.17319449210564 21.984684375530982 46-47 22.753166818424837 28.295349039825997 27.68887109403352 21.26261304771565 48-49 22.628436433790036 27.32490990246691 28.52011173433844 21.52654192940462 50-51 21.27283677920343 27.9428747213854 28.748231369672204 22.036057129738957 52-53 21.312649387420162 28.760641775112934 27.844847003740547 22.08186183372636 54-55 21.280002585120958 28.20532713201444 27.912258543295483 22.60241173956912 56-57 22.432257181481592 27.122844553012477 28.163463023214074 22.281435242291852 58-59 22.809169616178266 27.07804811486526 28.56992082963194 21.542861439324533 60-61 21.861879954296302 28.6460920221213 28.461116778290297 21.030911245292096 62-63 21.755640982133126 28.12468463241694 27.739081861177155 22.380592524272778 64-65 20.650355489603257 29.162786107572924 27.634665897955223 22.55219250486859 66-67 21.892968021175864 27.03651316929433 27.70011694936272 23.370401860167085 68-69 20.707919655467506 27.951144846971076 27.637893008467206 23.703042489094212 70-71 21.852632059437376 27.521641405429463 27.918780313032354 22.706946222100807 72-73 22.244080409827955 26.838325906043725 29.078101235723153 21.839492448405167 74-75 21.460519061808693 29.055151189487567 27.571826346678574 21.912503402025166 76-77 21.480589153295053 27.795626152447205 28.04059212613561 22.683192568122124 78-79 21.525557019098894 27.894162366008583 28.004357659652573 22.575922955239953 80-81 21.920557594532553 27.738158593515244 28.61877108308249 21.722512728869713 82-83 22.310460458530386 26.575817974436866 29.51180127630505 21.601920290727694 84-85 22.492033655829154 27.47268488106131 28.236376476110575 21.798904986998966 86-87 21.48590328251927 28.549744357089285 28.00265085394989 21.961701506441557 88-89 21.86090981586298 27.55682695907401 27.71069430971928 22.871568915343733 90-91 22.208759856794455 26.504216357177633 28.304098271896233 22.98292551413168 92-93 22.509645961593122 27.69422249577509 28.034121515255944 21.762010027375847 94-95 21.479257820328787 27.22051468349631 28.62362797497806 22.676599521196838 96-97 20.916821074500575 27.42048181917267 28.830366219050774 22.83233088727598 98-99 21.170305963424756 28.420981358119285 27.773650771272862 22.63506190718309 100-101 21.31834068353926 26.757831372034886 27.96129001223312 23.962537932192735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 123.0 1 82.5 2 82.0 3 110.5 4 71.5 5 66.5 6 105.5 7 168.0 8 268.5 9 325.5 10 303.0 11 314.0 12 404.0 13 543.0 14 699.5 15 880.0 16 1101.5 17 1266.5 18 1466.0 19 1697.5 20 1925.5 21 2265.5 22 2676.5 23 3117.0 24 3576.5 25 4210.0 26 5421.5 27 7486.5 28 11491.5 29 20686.5 30 34724.0 31 51331.0 32 71891.0 33 98324.5 34 132093.0 35 156504.5 36 174276.5 37 199202.0 38 200537.5 39 171964.0 40 143600.0 41 121261.0 42 106551.0 43 99819.5 44 96213.5 45 98694.5 46 105099.0 47 126942.5 48 171174.0 49 171219.0 50 129000.0 51 107846.5 52 98821.5 53 93063.0 54 87655.5 55 78526.5 56 70096.5 57 59077.5 58 48263.5 59 43962.0 60 37645.0 61 29486.0 62 23367.5 63 19495.0 64 15581.5 65 10930.5 66 8205.0 67 6512.0 68 5259.0 69 4291.5 70 3554.5 71 2924.0 72 2450.5 73 2011.5 74 1663.0 75 1425.0 76 1188.5 77 966.0 78 802.5 79 671.5 80 544.5 81 453.0 82 365.0 83 283.5 84 224.0 85 193.5 86 167.5 87 124.0 88 97.0 89 81.0 90 64.5 91 49.5 92 47.0 93 36.0 94 21.5 95 15.0 96 12.0 97 6.0 98 4.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.552726749761371E-4 2 0.003470454218600857 3 2.2210906999045488E-4 4 0.001166072617449888 5 0.001971217996165287 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.776363374880686E-5 12-13 6.940908437201714E-5 14-15 0.0034982178523496645 16-17 0.005066863159157251 18-19 0.003484336035475261 20-21 0.0 22-23 0.0 24-25 1.388181687440343E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.0822725311605143E-4 42-43 1.388181687440343E-5 44-45 6.107999424737508E-4 46-47 0.7362499215677346 48-49 0.5288972229147706 50-51 0.40716757074312704 52-53 0.34381095852834975 54-55 0.33482942301061075 56-57 0.301082726188936 58-59 0.30106884437206155 60-61 0.27769186475556623 62-63 0.2719586743864376 64-65 0.26862703833658075 66-67 0.25691078489458424 68-69 0.2376289412560379 70-71 0.2501642218936242 72-73 0.8137659869944034 74-75 0.5388643674305923 76-77 0.6320530041084624 78-79 0.3540279757479107 80-81 0.3048724621956481 82-83 0.2858127276270922 84-85 0.2777612738399382 86-87 0.25069173093485153 88-89 0.2534542124928578 90-91 0.2641709751198973 92-93 0.2213455700623627 94-95 0.18958397305372765 96-97 0.14709173160117872 98-99 0.15557352171143923 100-101 0.15129792211412296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3601834.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.2641041459968 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07909595588787 36.376067639367896 2 9.373056178292092 8.110337573278157 3 2.7277484773710463 3.540407826271954 4 1.1348910787501874 1.9640018330164302 5 0.5919236694531472 1.2804523640850773 6 0.3796689813788555 0.9855623010827593 7 0.24217850279239628 0.7334345176712266 8 0.17889473657771152 0.6191776411575018 9 0.11817888660424032 0.46016132921134184 >10 0.8686377366228734 7.481227940456753 >50 0.1276311697195529 3.889684196464254 >100 0.1460937759415448 13.263193845256882 >500 0.019084844640175926 5.753326621743074 >1k 0.011373796300654628 9.313073580855024 >5k 0.00115665725091403 3.494887304204787 >10k+ 3.8555241697134336E-4 2.735003485876801 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32613 0.905455387449838 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17729 0.49222146273259676 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13977 0.3880523089070735 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12034 0.3341075685331417 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 11197 0.3108694070853904 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10828 0.3006246262320807 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 9546 0.26503164776611027 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9186 0.2550367396165398 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8431 0.23407519613619063 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 8238 0.22871681482267092 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 7663 0.21275272541710694 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7501 0.20825501674980024 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7204 0.2000092175264046 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 7141 0.19826010860022977 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 7117 0.19759378139025838 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 6851 0.19020865481307578 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 6848 0.19012536391182935 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6383 0.17721527421863417 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6033 0.16749800240655177 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 5703 0.1583360032694455 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5672 0.1574753306232325 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 5596 0.15536529445832317 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 5304 0.14725831340367157 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5294 0.1469806770661835 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4854 0.13476467821670848 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4659 0.12935076963569114 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 4619 0.12824022428573886 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 4536 0.1259358426845879 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4469 0.12407567922341783 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4325 0.12007771596358965 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4222 0.11721806168746254 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 4139 0.11491368008631159 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 3901 0.10830593525409556 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3817 0.10597379001919577 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 3734 0.10366940841804481 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 3727 0.10347506298180316 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3678 0.10211464492811163 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 3676 0.102059117660614 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.776363374880686E-5 0.0 7 0.0 0.0 0.0 2.776363374880686E-5 0.0 8 0.0 0.0 0.0 2.776363374880686E-5 0.0 9 0.0 0.0 0.0 8.329090124642058E-5 0.0 10-11 0.0 0.0 0.0 1.6658180249284115E-4 0.0 12-13 0.0 0.0 0.0 1.6658180249284115E-4 2.776363374880686E-5 14-15 0.0 0.0 0.0 2.0822725311605146E-4 4.164545062321029E-5 16-17 0.0 0.0 0.0 2.2210906999045488E-4 8.329090124642058E-5 18-19 0.0 0.0 0.0 2.637545206136652E-4 8.329090124642058E-5 20-21 0.0 0.0 0.0 3.331636049856823E-4 1.1105453499522744E-4 22-23 0.0 1.388181687440343E-5 0.0 5.413908581017337E-4 1.1105453499522744E-4 24-25 0.0 2.776363374880686E-5 0.0 8.190271955898023E-4 1.1105453499522744E-4 26-27 0.0 2.776363374880686E-5 0.0 0.0013326544199427292 1.1105453499522744E-4 28-29 0.0 2.776363374880686E-5 0.0 0.002970708811122334 1.1105453499522744E-4 30-31 0.0 2.776363374880686E-5 0.0 0.009245290038352684 1.1105453499522744E-4 32-33 0.0 2.776363374880686E-5 0.0 0.02976261537872095 1.1105453499522744E-4 34-35 0.0 2.776363374880686E-5 0.0 0.062468175934815434 1.1105453499522744E-4 36-37 0.0 2.776363374880686E-5 0.0 0.11298410754076951 1.1105453499522744E-4 38-39 0.0 2.776363374880686E-5 0.0 0.18319833729150206 1.2493635186963087E-4 40-41 0.0 2.776363374880686E-5 0.0 0.25618893041711527 1.388181687440343E-4 42-43 0.0 2.776363374880686E-5 0.0 0.3355235138543309 1.388181687440343E-4 44-45 0.0 2.776363374880686E-5 1.388181687440343E-5 0.4314052230058354 1.388181687440343E-4 46-47 0.0 2.776363374880686E-5 2.776363374880686E-5 0.5522325570806428 1.388181687440343E-4 48-49 0.0 2.776363374880686E-5 2.776363374880686E-5 0.6735596365629288 1.388181687440343E-4 50-51 0.0 2.776363374880686E-5 2.776363374880686E-5 0.8003700337106041 1.6658180249284115E-4 52-53 0.0 2.776363374880686E-5 2.776363374880686E-5 0.9272498399426514 1.94345436241648E-4 54-55 0.0 2.776363374880686E-5 2.776363374880686E-5 1.0694829356377888 2.0822725311605146E-4 56-57 0.0 2.776363374880686E-5 2.776363374880686E-5 1.247656055220757 2.2210906999045488E-4 58-59 0.0 2.776363374880686E-5 2.776363374880686E-5 1.4448750275554065 2.2210906999045488E-4 60-61 0.0 2.776363374880686E-5 2.776363374880686E-5 1.6518112717021385 2.2210906999045488E-4 62-63 0.0 2.776363374880686E-5 2.776363374880686E-5 1.8584837613282568 2.2210906999045488E-4 64-65 0.0 2.776363374880686E-5 2.776363374880686E-5 2.0842437491566796 2.4987270373926174E-4 66-67 0.0 2.776363374880686E-5 2.776363374880686E-5 2.3333251893341003 2.4987270373926174E-4 68-69 0.0 4.164545062321029E-5 2.776363374880686E-5 2.610822708653425 2.4987270373926174E-4 70-71 0.0 5.552726749761372E-5 2.776363374880686E-5 2.901966053960288 2.4987270373926174E-4 72-73 0.0 5.552726749761372E-5 2.776363374880686E-5 3.2149732608443364 2.637545206136652E-4 74-75 0.0 5.552726749761372E-5 2.776363374880686E-5 3.523788159032315 2.776363374880686E-4 76-77 0.0 5.552726749761372E-5 2.776363374880686E-5 3.8683626174887573 2.776363374880686E-4 78-79 0.0 5.552726749761372E-5 2.776363374880686E-5 4.270907543212708 2.776363374880686E-4 80-81 0.0 5.552726749761372E-5 2.776363374880686E-5 4.716125174008575 2.9151815436247197E-4 82-83 0.0 5.552726749761372E-5 2.776363374880686E-5 5.173933612709526 3.053999712368754E-4 84-85 0.0 5.552726749761372E-5 2.776363374880686E-5 5.61977592526474 3.053999712368754E-4 86-87 0.0 5.552726749761372E-5 2.776363374880686E-5 6.10680558848631 3.053999712368754E-4 88-89 0.0 5.552726749761372E-5 2.776363374880686E-5 6.627901230317666 3.053999712368754E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4335 0.0 52.11544 2 ACCTGGG 4430 0.0 51.210247 3 GTACCTG 4850 0.0 47.06981 1 GTACCCG 355 0.0 42.693203 1 CCTGGGG 4345 0.0 41.420837 4 CTGGGGG 3690 0.0 41.071697 5 GTACACG 455 0.0 33.310085 1 TGGGGGG 7770 0.0 31.085808 6 TATTCGC 555 0.0 29.016642 9 GTACTAG 2100 0.0 28.417662 1 GCATCGT 1980 0.0 27.507538 8 GGGGGGA 7895 0.0 26.814419 7 TAGGCAT 2630 0.0 26.11147 5 TAACGCA 375 0.0 25.259445 4 GGGGAAT 8335 0.0 25.003935 9 CTAACGC 910 0.0 24.981869 3 TAACGCC 1005 0.0 24.97669 4 ATAACGC 375 0.0 23.996473 3 CATGGGG 6675 0.0 23.840376 4 GTACATG 10740 0.0 23.725487 1 >>END_MODULE