FastQCFastQC Report
Sat 18 Jun 2016
SRR1931091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054734
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA104550.34225565957625115No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC97360.3187184219640728No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA80120.26228142941414867No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC78960.2584840447646178No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC73300.23995542656087243No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG68440.22404569432232072No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC67680.22155775265538669No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC59610.19513974048149527No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA57910.18957460780545868No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC55050.18021209047989123No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA51750.1694091858734672No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT51650.169081825127818No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT49870.16325480385526203No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC48920.16014487677159453No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC41570.13608386196637742No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG40510.1326138380624958No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA39580.1295693831279581No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA37810.12377509792996706No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA35330.1156565514378666No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA32910.10773442139315567No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT32250.10557384047187088No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC31830.10419892534014419No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG36950.054.6064723
TACCTGG38450.051.9851462
GTACCTG40350.050.241591
CCTGGGG38350.046.9317974
CTGGGGG35300.045.217255
GTACCCG4600.033.979681
TGGGGGG81350.032.6046376
TAACGCA2800.030.4483244
GTACTAG21200.028.5981041
TATTCGC4350.028.311879
GGGGGGA84000.027.290727
CTGTGCG29100.026.858119
TAGTACT30500.026.399644
GTACACG4300.025.3350961
CTAGTAC36050.025.094413
GGGGAAT89800.024.9499289
ATAACGC3450.024.7116853
GTACATG100400.024.4848121
ACTGTGC34650.024.4680148
CATGGGG70050.024.408894
Produced by FastQC (version 0.11.5)