##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931091_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3054734
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.023117233775512	33.0	33.0	33.0	27.0	33.0
2	30.936775509749786	33.0	33.0	33.0	27.0	33.0
3	30.968072833837578	33.0	33.0	33.0	27.0	33.0
4	34.55968572058975	37.0	37.0	37.0	33.0	37.0
5	34.51757567107316	37.0	37.0	37.0	33.0	37.0
6	34.62083016066211	37.0	37.0	37.0	33.0	37.0
7	34.588698394033656	37.0	37.0	37.0	33.0	37.0
8	34.58811831079236	37.0	37.0	37.0	33.0	37.0
9	34.61154817407997	37.0	37.0	37.0	33.0	37.0
10-11	34.5075541765666	37.0	37.0	37.0	33.0	37.0
12-13	34.40437989036034	37.0	37.0	37.0	33.0	37.0
14-15	36.02065188000003	40.0	37.0	40.0	33.0	40.0
16-17	35.95574508287792	40.0	37.0	40.0	30.0	40.0
18-19	35.87871578998367	40.0	37.0	40.0	27.0	40.0
20-21	35.783823403281595	40.0	37.0	40.0	27.0	40.0
22-23	35.67890821262997	40.0	37.0	40.0	27.0	40.0
24-25	35.54253005335325	40.0	37.0	40.0	27.0	40.0
26-27	35.360164583888476	40.0	37.0	40.0	27.0	40.0
28-29	35.238463479962576	37.0	37.0	40.0	27.0	40.0
30-31	35.08956950097783	37.0	37.0	40.0	27.0	40.0
32-33	34.928204223346455	37.0	37.0	40.0	27.0	40.0
34-35	34.796613060253364	37.0	37.0	40.0	27.0	40.0
36-37	34.56776187386528	37.0	37.0	40.0	22.0	40.0
38-39	34.36455972925957	37.0	35.0	40.0	22.0	40.0
40-41	34.12259381667929	37.0	33.0	40.0	22.0	40.0
42-43	33.92159710141702	37.0	33.0	40.0	22.0	40.0
44-45	33.62867274204562	37.0	33.0	40.0	15.0	40.0
46-47	33.47237959180734	37.0	33.0	40.0	15.0	40.0
48-49	33.440673885189355	37.0	33.0	40.0	15.0	40.0
50-51	32.63349591159165	37.0	33.0	38.5	15.0	40.0
52-53	32.76375373436771	37.0	33.0	38.5	15.0	40.0
54-55	33.22267323439618	37.0	33.0	40.0	15.0	40.0
56-57	33.14899758866075	37.0	33.0	40.0	15.0	40.0
58-59	32.96386395673076	37.0	33.0	40.0	6.0	40.0
60-61	32.967359187412065	37.0	33.0	40.0	6.0	40.0
62-63	32.784371405169814	37.0	33.0	40.0	6.0	40.0
64-65	32.54586209470284	37.0	33.0	40.0	6.0	40.0
66-67	32.29023689132998	37.0	33.0	40.0	4.0	40.0
68-69	32.03777202859562	37.0	33.0	38.5	2.0	40.0
70-71	31.800569542225283	37.0	33.0	37.0	2.0	40.0
72-73	31.210043165787923	37.0	33.0	37.0	2.0	40.0
74-75	31.02496927719402	37.0	33.0	37.0	2.0	40.0
76-77	30.68154804968289	37.0	33.0	37.0	2.0	38.5
78-79	30.42358156225714	37.0	33.0	37.0	2.0	37.0
80-81	30.179322323973217	37.0	33.0	37.0	2.0	37.0
82-83	29.91321813945175	37.0	33.0	37.0	2.0	37.0
84-85	29.533502917111605	33.0	27.0	37.0	2.0	37.0
86-87	29.358368846518225	33.0	27.0	37.0	2.0	37.0
88-89	29.17551953787138	33.0	27.0	37.0	2.0	37.0
90-91	28.88460320931381	33.0	27.0	37.0	2.0	37.0
92-93	28.595452337257516	33.0	27.0	37.0	2.0	37.0
94-95	28.33433533001564	33.0	27.0	37.0	2.0	37.0
96-97	28.029990991032278	33.0	27.0	37.0	2.0	37.0
98-99	27.685256228529227	33.0	27.0	37.0	2.0	37.0
100-101	26.97237795500361	33.0	24.5	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.13118951753424568
1101	2	-0.08264854910515496
1101	3	-0.1712227302792897
1101	4	-0.1573903536981689
1101	5	-0.19515978371322262
1101	6	-0.1619320984958037
1101	7	-0.18647480594712107
1101	8	-0.09803012215127183
1101	9	-0.13343754722905743
1101	10-11	-0.09124498465788378
1101	12-13	-0.13698366068645385
1101	14-15	0.03333889923209199
1101	16-17	-0.028760905850823804
1101	18-19	0.02511302362764667
1101	20-21	0.03940352200287833
1101	22-23	0.04754659565813313
1101	24-25	0.08660200499539883
1101	26-27	0.0534675199687058
1101	28-29	0.06572759183980281
1101	30-31	0.03553127060710892
1101	32-33	0.11885918918158467
1101	34-35	0.03480045354618255
1101	36-37	0.04010335261233422
1101	38-39	0.19205667076894173
1101	40-41	0.16038679344259776
1101	42-43	0.1423327288138978
1101	44-45	-0.1443605598407416
1101	46-47	-0.0719762056093245
1101	48-49	0.007501302989844305
1101	50-51	0.0638113519497665
1101	52-53	0.11632179030114997
1101	54-55	0.260542150668428
1101	56-57	0.1888845395019274
1101	58-59	0.18466982838408086
1101	60-61	0.20804873606214613
1101	62-63	0.2416404722731329
1101	64-65	0.34554147584352535
1101	66-67	0.21698335466142993
1101	68-69	0.2794639309503282
1101	70-71	0.13947658276523
1101	72-73	0.1694641504217138
1101	74-75	0.19794282824919307
1101	76-77	0.11636210901488653
1101	78-79	0.02932811973715488
1101	80-81	0.16940118762452627
1101	82-83	0.0050913837239576765
1101	84-85	0.08265252143062796
1101	86-87	0.14358463184025183
1101	88-89	0.09922225861189915
1101	90-91	0.018531534292886676
1101	92-93	0.012554304633233926
1101	94-95	0.1111203318584586
1101	96-97	-0.10936868981107395
1101	98-99	-0.014315090390372376
1101	100-101	0.021398635215895467
1102	1	0.0409438708135319
1102	2	-0.02264655720284736
1102	3	-0.043811613838371954
1102	4	-0.024498457081961078
1102	5	0.09420162866746296
1102	6	0.04222213571939193
1102	7	-0.033069364395224454
1102	8	0.029682828465816158
1102	9	0.02785725024732244
1102	10-11	0.022168698897949923
1102	12-13	-0.008421610897023868
1102	14-15	0.014355612918492966
1102	16-17	0.12416282055122707
1102	18-19	0.08462974341170337
1102	20-21	0.055015056336777945
1102	22-23	0.04995161443892471
1102	24-25	0.15923402949986354
1102	26-27	0.11455260875064255
1102	28-29	0.0670886403934361
1102	30-31	0.1506764355691459
1102	32-33	0.1859598737455599
1102	34-35	0.11752359741844032
1102	36-37	0.08706321171573705
1102	38-39	0.1962800626127077
1102	40-41	0.10235714052140565
1102	42-43	0.15543990332079716
1102	44-45	0.1555353226126286
1102	46-47	0.05850432049678744
1102	48-49	0.021351375755259028
1102	50-51	-0.020391744848552662
1102	52-53	0.046621724039916046
1102	54-55	0.11575600577176459
1102	56-57	0.1796976523167615
1102	58-59	0.3453363127984659
1102	60-61	0.1978760604901808
1102	62-63	0.21322740864608392
1102	64-65	0.2282359607131994
1102	66-67	0.2820662218625287
1102	68-69	0.29189985470518565
1102	70-71	0.25732478964664907
1102	72-73	0.041535749146895284
1102	74-75	0.0842651668238048
1102	76-77	0.17755873416326295
1102	78-79	0.13790788322924286
1102	80-81	0.3149594487344629
1102	82-83	0.16845532053593715
1102	84-85	0.09256350575557803
1102	86-87	-0.03442537645256749
1102	88-89	-0.005814256209479396
1102	90-91	0.06658389083526828
1102	92-93	0.152183099410383
1102	94-95	0.15599250457671587
1102	96-97	0.1934548317096052
1102	98-99	0.2191295083022915
1102	100-101	0.24954995678210068
1103	1	-0.06533568496455189
1103	2	-0.18610976144898217
1103	3	-0.05073854110355214
1103	4	-0.1769961582992181
1103	5	-0.1480936416465397
1103	6	-0.08266661366665318
1103	7	-0.17466260169052106
1103	8	-0.1153493021393146
1103	9	-0.1705285330902626
1103	10-11	-0.17550049960265568
1103	12-13	-0.12122402219161899
1103	14-15	-0.13223670343589333
1103	16-17	-0.14636024057069363
1103	18-19	-0.06825251607754979
1103	20-21	-0.15295327186277063
1103	22-23	-0.21981089703118784
1103	24-25	-0.26641766112872034
1103	26-27	-0.16300892771195663
1103	28-29	-0.20973245481709313
1103	30-31	-0.12440623494057235
1103	32-33	-0.29023654034374147
1103	34-35	-0.2730860535663737
1103	36-37	-0.2533395726194172
1103	38-39	-0.2807641763426645
1103	40-41	-0.20055501980131396
1103	42-43	-0.25832819899835613
1103	44-45	-0.30074681513157486
1103	46-47	-0.3718410751794039
1103	48-49	-0.4125834287673058
1103	50-51	-0.4942240536450413
1103	52-53	-0.22139094020645445
1103	54-55	-0.23281412042808114
1103	56-57	-0.1024622825177417
1103	58-59	-0.07040265066469686
1103	60-61	-0.20220857326565778
1103	62-63	-0.1422048576966901
1103	64-65	-0.19617602922355815
1103	66-67	-0.09744769743353743
1103	68-69	-0.07571399691243741
1103	70-71	-0.10837674125699337
1103	72-73	-0.17155058323329442
1103	74-75	-0.21224901233465943
1103	76-77	-0.2216090636504653
1103	78-79	-0.17921508546542952
1103	80-81	-0.20951259273691036
1103	82-83	-0.22434370683623683
1103	84-85	-8.730292792051841E-4
1103	86-87	-0.17585453396534945
1103	88-89	-0.1953002878917509
1103	90-91	-0.30669505621753146
1103	92-93	-0.3801437113450632
1103	94-95	-0.39795309660970446
1103	96-97	-0.3427720567190029
1103	98-99	-0.3688478936639079
1103	100-101	-0.1955701153511349
1104	1	-0.022355368675029297
1104	2	0.04656140713888135
1104	3	-0.06980566436527269
1104	4	0.0053279944278585845
1104	5	-0.045430319424554
1104	6	0.03160773485401336
1104	7	0.07953616781585993
1104	8	0.01656269759393325
1104	9	-0.010749472042036246
1104	10-11	0.04166742204313323
1104	12-13	0.06045944922764335
1104	14-15	0.13449825013561423
1104	16-17	0.1566400887706223
1104	18-19	0.13915031909370867
1104	20-21	0.09465689879097994
1104	22-23	0.13923583653544824
1104	24-25	0.0398022482575513
1104	26-27	0.11466767949418966
1104	28-29	0.131351661925045
1104	30-31	-0.008644145438822193
1104	32-33	0.04940298322909342
1104	34-35	-0.004435148830424396
1104	36-37	0.08921018500509348
1104	38-39	0.039237997110653566
1104	40-41	0.23786817858922404
1104	42-43	-0.03466207288865775
1104	44-45	-0.07673993762516318
1104	46-47	0.014964822478411577
1104	48-49	0.015128200725598617
1104	50-51	0.022685346695645592
1104	52-53	0.0239160567512684
1104	54-55	0.24011442181063813
1104	56-57	0.3052003478991523
1104	58-59	0.3295197386628317
1104	60-61	0.31646739486544817
1104	62-63	0.2650899362401091
1104	64-65	0.2861367466607234
1104	66-67	0.2528495018137491
1104	68-69	0.3471726659161618
1104	70-71	0.2582258997435751
1104	72-73	0.12669096896243204
1104	74-75	0.13767015890831402
1104	76-77	0.07597175652131227
1104	78-79	0.15178828449498383
1104	80-81	0.0634247895412905
1104	82-83	-0.11357934141204495
1104	84-85	0.1368089240460506
1104	86-87	0.008927770092348908
1104	88-89	-0.0052352294106832176
1104	90-91	0.031793212839598795
1104	92-93	-0.07213712806353101
1104	94-95	-0.18625818963560903
1104	96-97	-0.3356303461226169
1104	98-99	-0.33278668071078954
1104	100-101	-0.2603203582029714
1105	1	0.1583047999363849
1105	2	0.12614590520747626
1105	3	0.16433188299321344
1105	4	0.2197549224399964
1105	5	0.18278737061539374
1105	6	0.15774680331783486
1105	7	0.08175555835075698
1105	8	0.16612545393761735
1105	9	0.18167702454548618
1105	10-11	0.13262277552592394
1105	12-13	0.12368798789166391
1105	14-15	0.2813979602212413
1105	16-17	0.20236442623362905
1105	18-19	0.2191551225842474
1105	20-21	0.3341364761727661
1105	22-23	0.34479113264321626
1105	24-25	0.30508539104501864
1105	26-27	0.29108810055571155
1105	28-29	0.3571765020496116
1105	30-31	0.24631149269526276
1105	32-33	0.3258242196979566
1105	34-35	0.3637638906197225
1105	36-37	0.3300715071955125
1105	38-39	0.3754182139941804
1105	40-41	0.2804902617474312
1105	42-43	0.2674863772073053
1105	44-45	0.15449031191172224
1105	46-47	0.2790081754630691
1105	48-49	0.17270344614467348
1105	50-51	0.4255191837283334
1105	52-53	0.4143983257185724
1105	54-55	0.5259221087298016
1105	56-57	0.5528588315824763
1105	58-59	0.5308382523383273
1105	60-61	0.49430143574996777
1105	62-63	0.4787396327973852
1105	64-65	0.5060810172751502
1105	66-67	0.4278136794865617
1105	68-69	0.4775829863045331
1105	70-71	0.5064881555012839
1105	72-73	0.5124757503480168
1105	74-75	0.5058603030056155
1105	76-77	0.6553023663941104
1105	78-79	0.5298196539567961
1105	80-81	0.5420396200218107
1105	82-83	0.5007418801571077
1105	84-85	0.48436073762317733
1105	86-87	0.5364451734795495
1105	88-89	0.4269136135633609
1105	90-91	0.5453704863991256
1105	92-93	0.6503849416775225
1105	94-95	0.62075787166523
1105	96-97	0.5282555591166691
1105	98-99	0.6116147695282343
1105	100-101	0.6762350201868088
1106	1	0.07439777576840356
1106	2	0.05894904051312011
1106	3	0.18563879139279393
1106	4	0.1086602738077076
1106	5	0.10943417609793471
1106	6	0.1270114615620841
1106	7	0.06975090637287451
1106	8	0.2252711043913962
1106	9	0.2464497626805482
1106	10-11	0.22348811775961508
1106	12-13	0.16841988845828837
1106	14-15	0.3702732542889038
1106	16-17	0.2698326031999656
1106	18-19	0.24827475170052793
1106	20-21	0.32616789295493476
1106	22-23	0.3202318225803893
1106	24-25	0.29815867031769727
1106	26-27	0.3687544645251819
1106	28-29	0.3469243605997434
1106	30-31	0.29724071996774626
1106	32-33	0.2881112828448451
1106	34-35	0.2945323697522042
1106	36-37	0.38055268952012256
1106	38-39	0.2521115923603574
1106	40-41	0.2892947671561501
1106	42-43	0.3091483441017857
1106	44-45	0.4875894532075833
1106	46-47	0.31271170215087096
1106	48-49	0.33622992152795916
1106	50-51	0.4036990177228219
1106	52-53	0.48285318931896626
1106	54-55	0.5504119046481648
1106	56-57	0.5039978226980537
1106	58-59	0.6057172531189607
1106	60-61	0.6198249691289774
1106	62-63	0.62005094889291
1106	64-65	0.6217841509876507
1106	66-67	0.5817367412952095
1106	68-69	0.6318191538394586
1106	70-71	0.5941864354377664
1106	72-73	0.36922892468566815
1106	74-75	0.4375305011395163
1106	76-77	0.4266094341080091
1106	78-79	0.4517256903760476
1106	80-81	0.6130166415876594
1106	82-83	0.6486374443156393
1106	84-85	0.7022652233831295
1106	86-87	0.5564334373252926
1106	88-89	0.5180740059014077
1106	90-91	0.5466735712552371
1106	92-93	0.6772091170931986
1106	94-95	0.7275201800233901
1106	96-97	0.7228375449164552
1106	98-99	0.8154942854251495
1106	100-101	0.8675096408859879
1107	1	0.002176202481255274
1107	2	-0.012785764336438632
1107	3	-0.011274448225361056
1107	4	0.02743418930293018
1107	5	0.020317287226944813
1107	6	-0.10043325588263485
1107	7	0.02925205431664324
1107	8	-0.1031290245005323
1107	9	-0.11469979223949878
1107	10-11	-0.10455715553155898
1107	12-13	-0.01729546176525787
1107	14-15	-0.021774314889810853
1107	16-17	0.08229111610929607
1107	18-19	0.001953508698953499
1107	20-21	-0.044006111153493066
1107	22-23	0.02498733069655401
1107	24-25	-0.04284708954052263
1107	26-27	-0.06906303255460955
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2316	84-85	-0.3442430848581992
2316	86-87	-0.343843132318959
2316	88-89	-0.321369509209557
2316	90-91	-0.21501986021571184
2316	92-93	-0.17955512958908315
2316	94-95	-0.33059457407497206
2316	96-97	-0.2898283411100877
2316	98-99	-0.36082611158611755
2316	100-101	-0.3280490744034097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98508.0
3	14266.0
4	11085.0
5	9547.0
6	9632.0
7	10318.0
8	10466.0
9	10843.0
10	10838.0
11	11155.0
12	11817.0
13	12514.0
14	12969.0
15	14512.0
16	16980.0
17	21521.0
18	19113.0
19	15003.0
20	14817.0
21	15639.0
22	17096.0
23	19304.0
24	22533.0
25	25799.0
26	29349.0
27	34664.0
28	41117.0
29	49711.0
30	61445.0
31	76589.0
32	98832.0
33	130212.0
34	179909.0
35	262985.0
36	408430.0
37	668176.0
38	576573.0
39	467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88643865442288	22.009651296895065	12.571892864777414	23.532017183904642
2	15.02799852682408	20.89529811351639	42.13414085198674	21.94256250767279
3	18.135651313095206	29.150048989615772	29.146611696160278	23.567688001128744
4	11.925913534581618	17.357116130187535	41.212806097879955	29.50416423735089
5	12.69610604908368	38.02657643515926	35.46992612273506	13.807391393022009
6	27.46517372707411	39.50926660062709	19.88503745334291	13.140522218955889
7	25.472823492978442	31.914693718012764	25.604095151983774	17.008387637025024
8	24.692428211425284	35.84292445757961	21.224794040986875	18.239853290008227
9	26.285856640872822	15.291413262169474	22.79989026867806	35.622839828279645
10-11	24.497210640469945	26.52774783790468	29.056888268649296	19.91815325297608
12-13	24.70053019381078	24.56076343194755	29.01099016214843	21.727716212093238
14-15	22.069870643928947	25.89645457936729	26.739437061209202	25.29423771549456
16-17	20.888480830466186	28.083446019550685	28.165454195160144	22.862618954822988
18-19	20.570947518117308	28.658257618452655	29.67501706029893	21.095777803131107
20-21	21.698223151344763	27.61086562692529	28.85531113347349	21.835600088256456
22-23	22.005582155434812	27.92405165228789	28.176577731481693	21.893788460795605
24-25	21.687951123715234	27.79912352405268	29.059544647600955	21.453380704631144
26-27	21.738783147730704	27.77191074574742	28.30167864043154	22.187627466090337
28-29	21.135064460604426	28.554663024669253	28.399084830299465	21.91118768442686
30-31	22.215109400687588	27.893918095650882	28.19463167660425	21.69634082705728
32-33	22.325315395710398	28.062001470504473	27.72997583422976	21.882707299555378
34-35	22.271660969498488	27.503000261233872	28.678405386524652	21.546933382742985
36-37	22.33014396670872	27.55190795663387	28.398970254038485	21.718977822618925
38-39	22.638730573594952	27.309857421300844	28.865344740327636	21.18606726477657
40-41	22.014584270063846	27.0811376260975	29.02964075869893	21.874637345139725
42-43	22.32499168912771	27.221687260116145	28.27996288383258	22.173358166923563
44-45	21.958231462144347	28.247704246107368	28.20228266756757	21.591781624180715
46-47	22.50206192518117	27.585649231536184	27.674572073249077	22.237716770033565
48-49	22.828302504848974	26.984146831628546	28.121806665941655	22.06574399758082
50-51	22.416283462952155	27.363210077140288	27.540581470555757	22.6799249893518
52-53	21.737381858752887	27.86303210466272	27.742807328177182	22.65677870840721
54-55	21.981667657908815	28.004572083884373	27.45677259219291	22.556987666013903
56-57	22.667515225970774	27.37695370274007	27.517304142192888	22.438226929096274
58-59	22.108108534018573	27.219192505168355	28.49640127395854	22.17629768685453
60-61	22.106990868090243	28.409868035919754	27.423120243403808	22.0600208525862
62-63	22.46627204563469	27.7363008134751	26.874529219953363	22.92289792093685
64-65	21.505412869780972	27.830995715324242	27.452316366936287	23.2112750479585
66-67	22.226324249148583	26.765299083705635	27.793447520636477	23.214929146509306
68-69	21.519026588813635	27.44759102188915	27.495772060542418	23.537610328754795
70-71	21.719099622999863	27.150162478560148	27.885311104686856	23.245426793753133
72-73	22.53235767448728	26.96340619429535	28.129346560984043	22.374889570233332
74-75	22.446282666935176	27.717563075716196	27.28954208911425	22.546612168234375
76-77	22.13657035154156	27.4971159808012	27.70853661448671	22.657777053170527
78-79	21.874826527518607	27.551503055907656	27.605552564666436	22.9681178519073
80-81	21.620776437366818	27.734868174925847	28.334105142580608	22.310250245126724
82-83	22.58044820857555	26.698598172639887	28.826672255747038	21.894281363037525
84-85	23.08316830155757	27.021168104947247	27.514597660271473	22.381065933223717
86-87	21.825079747708145	27.90113154622775	27.85460042796816	22.41918827809595
88-89	22.62042880495857	26.849623580252807	27.84979438554315	22.680153229245477
90-91	22.402310716321075	26.571367012463487	28.32271722490873	22.703605046306713
92-93	22.513174539022355	27.106958492328847	27.87192484739055	22.50794212125825
94-95	21.34391564583819	27.082773767487563	28.50645685694037	23.06685372973388
96-97	21.797910602421606	27.077870766283457	27.769676182221488	23.354542449073453
98-99	21.295557588542437	27.749855626422328	27.686467306822582	23.26811947821266
100-101	21.849271047327218	26.83868298578254	27.494308476399336	23.817737490490906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	46.0
2	42.0
3	58.0
4	45.5
5	50.0
6	77.5
7	125.5
8	211.0
9	283.5
10	281.5
11	286.5
12	370.0
13	520.5
14	668.5
15	863.5
16	1096.5
17	1305.5
18	1525.5
19	1780.5
20	2096.5
21	2396.5
22	2784.0
23	3242.0
24	3673.0
25	4281.0
26	5589.0
27	7585.0
28	11329.0
29	20498.5
30	33968.5
31	47401.5
32	63277.5
33	82127.0
34	104364.5
35	119829.5
36	128042.0
37	143918.0
38	144267.0
39	122385.5
40	102389.0
41	87973.5
42	81151.5
43	80728.5
44	82807.0
45	91294.0
46	101559.5
47	109134.0
48	115338.0
49	113876.0
50	107695.5
51	106637.0
52	104524.0
53	100462.0
54	95417.5
55	85137.5
56	76183.0
57	63912.5
58	51569.5
59	47499.0
60	40878.5
61	31518.0
62	24545.0
63	20160.0
64	15938.5
65	10874.5
66	7866.0
67	6187.5
68	5033.0
69	4070.5
70	3291.0
71	2696.0
72	2329.0
73	1986.0
74	1639.0
75	1413.5
76	1196.5
77	980.0
78	783.5
79	628.0
80	497.0
81	410.5
82	351.0
83	285.0
84	232.0
85	193.0
86	155.5
87	126.5
88	106.0
89	90.0
90	69.5
91	53.5
92	33.0
93	27.0
94	24.5
95	16.0
96	14.0
97	9.0
98	5.5
99	4.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.91041118473818E-4
2	0.003568232127576411
3	1.63680372824606E-4
4	0.0010802904606423997
5	0.0023897334432392476
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.63680372824606E-5
12-13	6.54721491298424E-5
14-15	0.0033881837174693443
16-17	0.005254139967669853
18-19	0.004173849507027453
20-21	0.0
22-23	0.0
24-25	3.27360745649212E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.473123355421454E-4
42-43	1.63680372824606E-5
44-45	6.056173794510422E-4
46-47	0.7125825030919223
48-49	0.5135799058117663
50-51	0.3917198682438471
52-53	0.3280318351777929
54-55	0.3186202137403781
56-57	0.2876518872019626
58-59	0.287913775798482
60-61	0.2654731966842285
62-63	0.2595643352252602
64-65	0.25629072776876805
66-67	0.24421111625431216
68-69	0.22438942310525237
70-71	0.2375002209685033
72-73	0.7844054506873593
74-75	0.5155113342110966
76-77	0.6091037713922064
78-79	0.33727977624238314
80-81	0.29005798868248434
82-83	0.2722823001937321
84-85	0.2651130998640143
86-87	0.23933344114413893
88-89	0.24239426411595905
90-91	0.25250971115651966
92-93	0.21049295945244334
94-95	0.179884729734242
96-97	0.13871911596885358
98-99	0.1469031346100839
100-101	0.14287659743859857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3054734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.7035536078264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42849909293086	34.36539938857434
2	9.053014033283015	7.369796840322797
3	2.583565134745434	3.1548084588426604
4	1.0733489659793898	1.7475646870658847
5	0.5878182225093855	1.1963145265784003
6	0.3644422372777766	0.8900456485195299
7	0.24776987006358012	0.705957993197598
8	0.17437215538000655	0.5678053099377864
9	0.13665879985478632	0.5006248907283462
>10	0.9685059936260426	7.978950213484523
>50	0.15172694689419058	4.407260826224947
>100	0.18631597260881644	16.364253717865658
>500	0.029039498981378847	7.971710694460382
>1k	0.013955092566051396	10.000533737824377
>5k	8.873180244310136E-4	2.435697428846832
>10k+	8.066527494827397E-5	0.3432756375260319
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10455	0.34225565957625115	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9736	0.3187184219640728	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8012	0.26228142941414867	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	7896	0.2584840447646178	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7330	0.23995542656087243	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6844	0.22404569432232072	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6768	0.22155775265538669	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	5961	0.19513974048149527	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5791	0.18957460780545868	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	5505	0.18021209047989123	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5175	0.1694091858734672	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	5165	0.169081825127818	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4987	0.16325480385526203	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	4892	0.16014487677159453	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	4157	0.13608386196637742	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	4051	0.1326138380624958	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	3958	0.1295693831279581	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	3781	0.12377509792996706	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	3533	0.1156565514378666	No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA	3291	0.10773442139315567	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	3225	0.10557384047187088	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3183	0.10419892534014419	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	3.27360745649212E-5	6.54721491298424E-5
14-15	0.0	0.0	0.0	3.27360745649212E-5	6.54721491298424E-5
16-17	0.0	0.0	0.0	3.27360745649212E-5	6.54721491298424E-5
18-19	0.0	0.0	0.0	3.27360745649212E-5	6.54721491298424E-5
20-21	0.0	0.0	0.0	6.54721491298424E-5	9.82082236947636E-5
22-23	0.0	0.0	0.0	9.82082236947636E-5	9.82082236947636E-5
24-25	0.0	0.0	0.0	1.6368037282460602E-4	9.82082236947636E-5
26-27	0.0	0.0	0.0	2.782566338018302E-4	9.82082236947636E-5
28-29	0.0	0.0	0.0	0.0012276027961845449	9.82082236947636E-5
30-31	0.0	0.0	0.0	0.005483292489624301	9.82082236947636E-5
32-33	0.0	0.0	0.0	0.01856135427831032	9.82082236947636E-5
34-35	0.0	0.0	0.0	0.03913597714236329	1.6368037282460602E-4
36-37	0.0	0.0	0.0	0.07183931563271957	1.6368037282460602E-4
38-39	0.0	0.0	0.0	0.12050149047347494	1.6368037282460602E-4
40-41	0.0	0.0	0.0	0.17034216399856747	1.6368037282460602E-4
42-43	0.0	0.0	0.0	0.22535513730491755	1.6368037282460602E-4
44-45	0.0	0.0	0.0	0.2918584727835549	1.6368037282460602E-4
46-47	0.0	0.0	0.0	0.3747953176937828	1.6368037282460602E-4
48-49	0.0	0.0	0.0	0.46830591468848026	1.8004841010706661E-4
50-51	0.0	0.0	0.0	0.5617837756086127	1.964164473895272E-4
52-53	0.0	0.0	0.0	0.6544596027019046	1.964164473895272E-4
54-55	0.0	0.0	0.0	0.7630287939964657	1.964164473895272E-4
56-57	0.0	0.0	0.0	0.8934656831003944	2.291525219544484E-4
58-59	0.0	0.0	0.0	1.0445426672175055	2.291525219544484E-4
60-61	0.0	0.0	0.0	1.2044583914671456	2.291525219544484E-4
62-63	0.0	0.0	0.0	1.3674513067258884	2.291525219544484E-4
64-65	0.0	0.0	0.0	1.5403141484659548	2.45520559236909E-4
66-67	0.0	0.0	0.0	1.7349464797916938	2.618885965193696E-4
68-69	0.0	0.0	0.0	1.9539999227428642	2.618885965193696E-4
70-71	0.0	1.63680372824606E-5	0.0	2.1939880853783014	2.618885965193696E-4
72-73	0.0	3.27360745649212E-5	0.0	2.4552874325555023	2.9462467108429084E-4
74-75	0.0	3.27360745649212E-5	0.0	2.7118727849953546	3.2736074564921204E-4
76-77	0.0	3.27360745649212E-5	0.0	3.000621330695242	3.2736074564921204E-4
78-79	0.0	3.27360745649212E-5	0.0	3.320403675082675	3.2736074564921204E-4
80-81	0.0	3.27360745649212E-5	0.0	3.6795675171717077	3.2736074564921204E-4
82-83	0.0	3.27360745649212E-5	0.0	4.06726084824407	3.2736074564921204E-4
84-85	0.0	3.27360745649212E-5	0.0	4.449667303274197	3.2736074564921204E-4
86-87	0.0	3.27360745649212E-5	0.0	4.869474723494746	3.4372878293167263E-4
88-89	0.0	3.27360745649212E-5	0.0	5.320839719595879	3.6009682021413323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGGG	3695	0.0	54.606472	3
TACCTGG	3845	0.0	51.985146	2
GTACCTG	4035	0.0	50.24159	1
CCTGGGG	3835	0.0	46.931797	4
CTGGGGG	3530	0.0	45.21725	5
GTACCCG	460	0.0	33.97968	1
TGGGGGG	8135	0.0	32.604637	6
TAACGCA	280	0.0	30.448324	4
GTACTAG	2120	0.0	28.598104	1
TATTCGC	435	0.0	28.31187	9
GGGGGGA	8400	0.0	27.29072	7
CTGTGCG	2910	0.0	26.85811	9
TAGTACT	3050	0.0	26.39964	4
GTACACG	430	0.0	25.335096	1
CTAGTAC	3605	0.0	25.09441	3
GGGGAAT	8980	0.0	24.949928	9
ATAACGC	345	0.0	24.711685	3
GTACATG	10040	0.0	24.484812	1
ACTGTGC	3465	0.0	24.468014	8
CATGGGG	7005	0.0	24.40889	4
>>END_MODULE