##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931090_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3830910
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.368878934770066	33.0	33.0	33.0	27.0	33.0
2	31.290532275621196	33.0	33.0	33.0	27.0	33.0
3	31.31184966496211	33.0	33.0	33.0	27.0	33.0
4	34.96538315961482	37.0	37.0	37.0	33.0	37.0
5	34.9601653393058	37.0	37.0	37.0	33.0	37.0
6	35.04930316817675	37.0	37.0	37.0	33.0	37.0
7	35.02147453216077	37.0	37.0	37.0	33.0	37.0
8	35.01858331310315	37.0	37.0	37.0	33.0	37.0
9	35.031609199902896	37.0	37.0	37.0	33.0	37.0
10-11	34.9458780028766	37.0	37.0	37.0	33.0	37.0
12-13	34.874600551827115	37.0	37.0	37.0	33.0	37.0
14-15	36.603400236497336	40.0	37.0	40.0	33.0	40.0
16-17	36.57470144169401	40.0	37.0	40.0	33.0	40.0
18-19	36.52380348272342	40.0	37.0	40.0	33.0	40.0
20-21	36.44532787248983	40.0	37.0	40.0	33.0	40.0
22-23	36.374717625838244	40.0	37.0	40.0	33.0	40.0
24-25	36.265447374122594	40.0	37.0	40.0	33.0	40.0
26-27	36.10702431015085	40.0	37.0	40.0	33.0	40.0
28-29	36.01803801185619	40.0	37.0	40.0	33.0	40.0
30-31	35.901648824952815	40.0	37.0	40.0	33.0	40.0
32-33	35.758979850740424	40.0	37.0	40.0	27.0	40.0
34-35	35.644299787778884	40.0	37.0	40.0	27.0	40.0
36-37	35.45640409197814	38.5	37.0	40.0	27.0	40.0
38-39	35.30351235085136	37.0	37.0	40.0	27.0	40.0
40-41	35.11931512356072	37.0	37.0	40.0	27.0	40.0
42-43	34.973345758579555	37.0	37.0	40.0	27.0	40.0
44-45	34.746618949544626	37.0	37.0	40.0	27.0	40.0
46-47	34.598946725451654	37.0	37.0	40.0	22.0	40.0
48-49	34.615165195736786	37.0	37.0	40.0	22.0	40.0
50-51	33.826304716111835	37.0	35.0	38.5	22.0	40.0
52-53	33.95539049468664	37.0	35.0	38.5	22.0	40.0
54-55	34.485434009151874	37.0	37.0	40.0	22.0	40.0
56-57	34.45053564296734	37.0	37.0	40.0	22.0	40.0
58-59	34.3336078111989	37.0	35.0	40.0	22.0	40.0
60-61	34.35296744115628	37.0	35.0	40.0	22.0	40.0
62-63	34.22043744175666	37.0	33.0	40.0	22.0	40.0
64-65	34.03119324129254	37.0	33.0	40.0	22.0	40.0
66-67	33.80082486928693	37.0	33.0	40.0	22.0	40.0
68-69	33.590205199286856	37.0	33.0	40.0	22.0	40.0
70-71	33.348437708011936	37.0	33.0	40.0	18.5	40.0
72-73	32.74742893986024	37.0	33.0	40.0	15.0	40.0
74-75	32.57451558506987	37.0	33.0	37.0	15.0	40.0
76-77	32.211717059393195	37.0	33.0	37.0	10.5	40.0
78-79	31.92903435476166	37.0	33.0	37.0	6.0	38.5
80-81	31.630284188352114	37.0	33.0	37.0	6.0	37.0
82-83	31.344218997574988	37.0	33.0	37.0	4.0	37.0
84-85	30.95899551281549	37.0	33.0	37.0	2.0	37.0
86-87	30.802857415078925	37.0	33.0	37.0	2.0	37.0
88-89	30.58768699865045	37.0	33.0	37.0	2.0	37.0
90-91	30.305132592517182	37.0	33.0	37.0	2.0	37.0
92-93	30.030784330615965	33.0	33.0	37.0	2.0	37.0
94-95	29.785849576210353	33.0	33.0	37.0	2.0	37.0
96-97	29.498810073846684	33.0	33.0	37.0	2.0	37.0
98-99	29.196615295060443	33.0	27.0	37.0	2.0	37.0
100-101	28.51968566215338	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.058969234550289684
1101	2	0.029755441952140416
1101	3	0.06751889975043923
1101	4	0.07432690240015205
1101	5	-0.007769831130680416
1101	6	0.045441354193421546
1101	7	0.10215813357940107
1101	8	0.038165853660672155
1101	9	0.11971629687320728
1101	10-11	-0.0425898491486052
1101	12-13	0.014256758772553724
1101	14-15	0.037702149948621866
1101	16-17	0.04208335568844035
1101	18-19	0.11656653368039116
1101	20-21	0.15279241311195335
1101	22-23	0.07755653377615346
1101	24-25	0.13557782327301027
1101	26-27	0.07263815811666774
1101	28-29	0.04229255345445182
1101	30-31	0.0650002790321409
1101	32-33	-0.004754449132136074
1101	34-35	0.07614834436808593
1101	36-37	0.023628329145182647
1101	38-39	0.12118901226961043
1101	40-41	-0.12401101765902922
1101	42-43	-0.12649598528081185
1101	44-45	-0.0988363468823863
1101	46-47	-0.17673787359336757
1101	48-49	-0.14163807126154637
1101	50-51	-0.062042153445936776
1101	52-53	0.07671257544402721
1101	54-55	0.10933992029175243
1101	56-57	0.10843486773832467
1101	58-59	0.1898298377325105
1101	60-61	0.07175663100376539
1101	62-63	0.07188957234314586
1101	64-65	0.0824413797023098
1101	66-67	0.2196134943047099
1101	68-69	0.07291063113898844
1101	70-71	0.060307359058313637
1101	72-73	0.024948937131490823
1101	74-75	0.059102444478092764
1101	76-77	-0.06602815399867978
1101	78-79	0.02482868081443712
1101	80-81	-0.008253976330905743
1101	82-83	-0.05655808308992505
1101	84-85	0.06430116737893599
1101	86-87	6.282034915301438E-4
1101	88-89	-7.708251535838428E-4
1101	90-91	-0.1106429422438282
1101	92-93	-0.10286709244948966
1101	94-95	-0.010153335514864636
1101	96-97	-0.12552123535196813
1101	98-99	-0.13071260872000678
1101	100-101	0.012731576435118797
1102	1	0.12093026707017174
1102	2	0.11888379068533794
1102	3	0.12604192242274337
1102	4	0.21235204125154894
1102	5	0.13285562179014931
1102	6	0.17971928924229985
1102	7	0.25921926631252035
1102	8	0.12928552492934386
1102	9	0.1442005931799386
1102	10-11	0.15388026040339042
1102	12-13	0.11793650368753816
1102	14-15	0.25977499047750285
1102	16-17	0.315970117834695
1102	18-19	0.2770929346157729
1102	20-21	0.3270429302450424
1102	22-23	0.3146195635134674
1102	24-25	0.26356594838131997
1102	26-27	0.30847434028047616
1102	28-29	0.2731302182068305
1102	30-31	0.3465984962027733
1102	32-33	0.33997074897848023
1102	34-35	0.3316718399617571
1102	36-37	0.3912932721087401
1102	38-39	0.2904344079346046
1102	40-41	0.33545740453431705
1102	42-43	0.20396821785110575
1102	44-45	0.3941024095781742
1102	46-47	0.39372687913789406
1102	48-49	0.3767722658157169
1102	50-51	0.2897408310469416
1102	52-53	0.4601744662696632
1102	54-55	0.47358156848699906
1102	56-57	0.4977440967080611
1102	58-59	0.5489846780406609
1102	60-61	0.539238344636793
1102	62-63	0.6100087635988487
1102	64-65	0.5458901865177026
1102	66-67	0.5593242632163538
1102	68-69	0.37733667877949273
1102	70-71	0.4755550621541076
1102	72-73	0.3586180579365319
1102	74-75	0.33109215810581105
1102	76-77	0.3942272590008429
1102	78-79	0.41715290542505556
1102	80-81	0.33727310495527973
1102	82-83	0.3102676571295362
1102	84-85	0.4383764775590677
1102	86-87	0.38683303828152305
1102	88-89	0.46309266323325105
1102	90-91	0.45541854462628706
1102	92-93	0.4012871251271406
1102	94-95	0.26278594395152766
1102	96-97	0.2755639574222215
1102	98-99	0.23862466754541245
1102	100-101	0.3187435727705328
1103	1	-0.017250456154943805
1103	2	-0.014556006542388644
1103	3	-0.08412259156187929
1103	4	-0.08237388496097964
1103	5	-0.12637014648601763
1103	6	-0.06735630306908291
1103	7	-0.05117963390338787
1103	8	0.03048724218290033
1103	9	-0.08909892920849671
1103	10-11	-0.08789816805811057
1103	12-13	-5.658410086439858E-4
1103	14-15	0.1297517970143005
1103	16-17	0.13456336349528186
1103	18-19	0.041284734066287854
1103	20-21	0.03843428620624678
1103	22-23	0.04268083548321755
1103	24-25	0.04676994178635141
1103	26-27	0.06956849403344734
1103	28-29	0.06677719058180998
1103	30-31	-0.04037373034633873
1103	32-33	-0.056439425925610465
1103	34-35	-0.018386181220726883
1103	36-37	-0.1094550910735066
1103	38-39	-0.04854355202127181
1103	40-41	-0.03441645984987929
1103	42-43	-0.018297773664023964
1103	44-45	-0.05098797938555322
1103	46-47	-0.02594901443667652
1103	48-49	-0.05507602655367094
1103	50-51	0.007507095573501488
1103	52-53	0.141730859890437
1103	54-55	0.19492349887292448
1103	56-57	0.3037940766449765
1103	58-59	0.242038780333381
1103	60-61	0.20039117833530895
1103	62-63	0.26031794028510546
1103	64-65	0.13070718213784005
1103	66-67	0.13966809083991194
1103	68-69	0.20478403360396413
1103	70-71	0.23178049821356694
1103	72-73	0.18283217861458212
1103	74-75	0.16413362048643165
1103	76-77	0.06332535899473868
1103	78-79	0.08578595204913597
1103	80-81	0.14046709683598024
1103	82-83	0.23518609559089043
1103	84-85	0.20560321513421087
1103	86-87	0.08176278078354926
1103	88-89	0.07178598417627668
1103	90-91	0.1347932650689252
1103	92-93	0.10687677977852417
1103	94-95	0.06002384214044554
1103	96-97	-0.060504020922316215
1103	98-99	-0.07116591918066106
1103	100-101	-0.09278354675376832
1104	1	-0.1031843384965292
1104	2	-0.1509334084534828
1104	3	-0.17443530516672823
1104	4	-0.22611937930974335
1104	5	-0.22929066485195193
1104	6	-0.2988510192928544
1104	7	-0.1788305138580455
1104	8	-0.20468712165418168
1104	9	-0.1568405683546814
1104	10-11	-0.20511956505522022
1104	12-13	-0.23575472413653387
1104	14-15	-0.18409427359071628
1104	16-17	-0.15935355969183007
1104	18-19	-0.18215979445769648
1104	20-21	-0.19397592385246298
1104	22-23	-0.1994062678053936
1104	24-25	-0.17332794859572687
1104	26-27	-0.1951767994586433
1104	28-29	-0.25070411339842025
1104	30-31	-0.28821090097643065
1104	32-33	-0.20622922707750035
1104	34-35	-0.22394103611345173
1104	36-37	-0.210976813956691
1104	38-39	-0.20369910695401927
1104	40-41	-0.06670199085109374
1104	42-43	-0.3237493975668997
1104	44-45	-0.22578704485704293
1104	46-47	-0.3911498555434818
1104	48-49	-0.3767714281813923
1104	50-51	-0.3367840983403809
1104	52-53	-0.23513157975524734
1104	54-55	-0.16605686076952253
1104	56-57	-0.05382236183916689
1104	58-59	-0.12436365859606724
1104	60-61	-0.2716518688317535
1104	62-63	-0.17905047445746192
1104	64-65	-0.14017560631337034
1104	66-67	-0.047462212204543164
1104	68-69	-0.11968692703591444
1104	70-71	-0.2678257682033518
1104	72-73	-0.2955489866344081
1104	74-75	-0.2791201253348916
1104	76-77	-0.36851529003702055
1104	78-79	-0.33482906642500865
1104	80-81	-0.2550694863272476
1104	82-83	-0.280315290942994
1104	84-85	-0.22543311189782855
1104	86-87	-0.31292969830166584
1104	88-89	-0.3234449153658012
1104	90-91	-0.34218556245746967
1104	92-93	-0.4367112600627223
1104	94-95	-0.40952332725958485
1104	96-97	-0.22789478369501381
1104	98-99	-0.2716370760061295
1104	100-101	-0.1432435708254296
1105	1	-0.054355777152000684
1105	2	-0.07140438537401295
1105	3	-0.028767572398098906
1105	4	-0.0578310005488234
1105	5	-0.09764344635926392
1105	6	-0.003578253649720864
1105	7	-0.059531157521654166
1105	8	-0.012658507914203199
1105	9	0.05634707650601456
1105	10-11	0.020313879982303717
1105	12-13	0.005643870646487414
1105	14-15	0.06424035143611917
1105	16-17	0.05973821604106888
1105	18-19	0.028882547619126342
1105	20-21	0.12111560445745795
1105	22-23	0.07899461564019106
1105	24-25	0.14004289842773687
1105	26-27	0.1507834745986898
1105	28-29	0.09585525174332332
1105	30-31	0.02559475554954105
1105	32-33	0.047169928045796894
1105	34-35	0.0919282372270871
1105	36-37	0.06929848049485088
1105	38-39	0.07473870800998128
1105	40-41	0.032206801033431987
1105	42-43	-0.010518453684618123
1105	44-45	0.1360989004260844
1105	46-47	-0.06141306993185225
1105	48-49	0.003948863777729628
1105	50-51	0.021228767654598357
1105	52-53	0.028522530195161266
1105	54-55	0.18355326610753053
1105	56-57	0.15123925739870003
1105	58-59	0.20106924065547815
1105	60-61	0.19150230115511135
1105	62-63	0.1425428203797665
1105	64-65	0.19543092565331932
1105	66-67	0.2217589148071255
1105	68-69	0.16892925078843746
1105	70-71	0.2292442706929947
1105	72-73	0.08039440444308354
1105	74-75	0.11496919436106623
1105	76-77	0.0940254165011325
1105	78-79	0.1913861260737093
1105	80-81	0.23141939965078606
1105	82-83	0.236808016665627
1105	84-85	0.16661041999244475
1105	86-87	0.10738172718217243
1105	88-89	0.003634438278051988
1105	90-91	0.10176148902469961
1105	92-93	0.224932356242455
1105	94-95	0.12740582608080686
1105	96-97	0.11412873604243146
1105	98-99	0.05522123431322612
1105	100-101	-0.04334581473354859
1106	1	-0.11388243107191798
1106	2	-0.019075258854311272
1106	3	0.05231463836297223
1106	4	0.013189858034550639
1106	5	-0.11485199458913797
1106	6	-0.13403291349369084
1106	7	-0.043211217919271405
1106	8	-0.010565418061176501
1106	9	-0.03401504886532791
1106	10-11	-0.09358993796862336
1106	12-13	-0.018899386833076903
1106	14-15	0.01485249922514953
1106	16-17	0.04031262978210037
1106	18-19	-0.03776179776962607
1106	20-21	0.12274291239562984
1106	22-23	0.06035957865822894
1106	24-25	0.08302840068542139
1106	26-27	0.08708539326439535
1106	28-29	0.12083059227538939
1106	30-31	0.15186453736024674
1106	32-33	0.17439398064494327
1106	34-35	0.2108092903671519
1106	36-37	0.1276756850223606
1106	38-39	0.13040742071343914
1106	40-41	0.26987026944183157
1106	42-43	0.21658049369598587
1106	44-45	0.15081084023673696
1106	46-47	0.20391116229814799
1106	48-49	0.2939150636741701
1106	50-51	0.256985357734699
1106	52-53	0.28135576566198495
1106	54-55	0.3319239333450028
1106	56-57	0.3070977973846425
1106	58-59	0.3477558325579153
1106	60-61	0.2846547791062193
1106	62-63	0.40219566424556064
1106	64-65	0.3621099823165821
1106	66-67	0.33496344520697363
1106	68-69	0.45763239418360513
1106	70-71	0.45709450022128806
1106	72-73	0.293655051403384
1106	74-75	0.3928460358664978
1106	76-77	0.32773968196718073
1106	78-79	0.3902464392272549
1106	80-81	0.4881136135705866
1106	82-83	0.48404405911751525
1106	84-85	0.4452628415377333
1106	86-87	0.49304893573040687
1106	88-89	0.47619423567990893
1106	90-91	0.524310945359364
1106	92-93	0.6073533374723326
1106	94-95	0.7331541577701763
1106	96-97	0.7200820088817252
1106	98-99	0.7773659427240567
1106	100-101	0.7298494159373341
1107	1	0.09233590038364525
1107	2	0.11702204816577577
1107	3	0.14523400232742034
1107	4	0.12164094682177051
1107	5	0.06965755136657492
1107	6	0.12765846543230452
1107	7	0.12568307575887872
1107	8	0.13237251364568436
1107	9	0.15003591767056434
1107	10-11	0.14995715535810206
1107	12-13	0.15591378552870339
1107	14-15	0.2924555159629634
1107	16-17	0.2516598208222618
1107	18-19	0.22659999496507055
1107	20-21	0.30478175731773405
1107	22-23	0.19422615491441064
1107	24-25	0.2854013882899409
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2316	90-91	-0.315281417720211
2316	92-93	-0.3130556787890768
2316	94-95	-0.1582130190983655
2316	96-97	-0.23826605902258535
2316	98-99	-0.17635832423489362
2316	100-101	-0.24752152310690434
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94314.0
3	14178.0
4	10631.0
5	8886.0
6	8937.0
7	9616.0
8	9767.0
9	10203.0
10	10510.0
11	10669.0
12	11444.0
13	12134.0
14	12902.0
15	14774.0
16	17047.0
17	21100.0
18	18542.0
19	14581.0
20	14991.0
21	16154.0
22	17701.0
23	20071.0
24	23465.0
25	27051.0
26	30712.0
27	35701.0
28	43367.0
29	52659.0
30	64674.0
31	81823.0
32	105694.0
33	141831.0
34	196164.0
35	285345.0
36	449680.0
37	864815.0
38	1047949.0
39	828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18462047682964	23.80292705134008	13.603798181412863	22.408654290417413
2	13.512244975207238	22.526603763487877	44.045129484918775	19.916021776386117
3	17.04893581031696	31.43123697432663	30.93936770927741	20.580459506078995
4	10.513059195001441	18.715908473910847	44.37515923300853	26.395873098079182
5	11.064590803440721	39.173660810311304	37.305864221379004	12.455884164868968
6	24.464500601684716	42.21490455270418	21.516036659697043	11.804558185914052
7	23.10565896875677	33.36243868950197	27.92216470760211	15.609737634139147
8	22.175462226990454	37.48803809016656	23.038207632129183	17.298292050713798
9	24.966235176498532	16.640484897844114	24.587134649469707	33.80614527618764
10-11	22.08041801056042	27.924123993580636	31.75465189975849	18.240806096100453
12-13	22.772190905282173	26.195167982500216	31.15006767330435	19.88257343891326
14-15	20.210618588174395	27.928531740474188	28.847160624768403	23.01368904658301
16-17	18.91421830442496	30.112375624033472	30.237443365797173	20.7359627057444
18-19	18.736729938329415	31.008496624622317	31.166441928637333	19.088331508410935
20-21	19.589457857271512	29.250491397605266	30.412225815798333	20.74782492932489
22-23	19.51762113962479	30.103904816349118	29.79067636671183	20.587797677314267
24-25	19.74920312646424	29.871865920072754	30.37471002313028	20.00422093033272
26-27	19.911430965488616	29.310542403763073	30.339344959813726	20.43868167093458
28-29	19.414329754549183	30.76040679629644	29.544272770699394	20.280990678454987
30-31	20.601371475706816	29.599716516441266	29.252436105259587	20.546475902592334
32-33	20.508717249948447	29.927432385516756	29.37591851544411	20.18793184909069
34-35	20.89110681274162	29.054741562709648	30.060155419991595	19.993996204557142
36-37	20.897710987728765	29.31845175167258	30.277793004795207	19.506044255803452
38-39	21.02022756995074	29.098438752150273	30.65612347979984	19.225210198099145
40-41	20.25858929792234	29.335722849533308	30.435385826989524	19.970302025554822
42-43	20.666669277012513	29.299644209861363	29.55049844553905	20.483188067587076
44-45	20.15806789318031	30.103131508367976	29.574076280797218	20.164724317654496
46-47	21.211651413822118	29.34172854525905	29.071557465399206	20.375062575519628
48-49	20.943161016448727	28.49953744103193	29.982586787216313	20.574714755303024
50-51	20.49884570749317	29.077122990636568	29.24544065927369	21.17859064259657
52-53	19.837418307394838	29.71195035819078	29.441321399621778	21.009309934792604
54-55	20.281786239528127	29.793895439062435	29.028540073049385	20.895778248360056
56-57	20.86035779510241	28.87409094777082	29.470363751808286	20.795187505318488
58-59	21.120899348294543	28.368563132166074	30.01354992262798	20.496987596911403
60-61	20.621488263573827	29.984203444039238	29.182229483161137	20.212078809225794
62-63	20.775383988776873	29.357595483003873	28.5946633095365	21.27235721868275
64-65	19.829709522802418	29.583975487845482	28.85407876304289	21.732236226309208
66-67	20.57130616344748	27.996613927545695	29.422033182358504	22.010046726648323
68-69	19.70858498401001	29.070330313263554	28.87713428817986	22.343950414546573
70-71	20.436034366371818	28.53779883495796	29.452188958257086	21.573977840413132
72-73	20.9691927127097	28.151190336287783	30.01501943920496	20.864597511797555
74-75	20.49630614841416	29.45022338960341	28.92789394357944	21.125576518402987
76-77	20.198841387949038	28.918709317419296	29.760188690199875	21.12226060443179
78-79	20.025940792538105	28.85148202037434	29.540817582942687	21.581759604144867
80-81	20.256428753883096	28.81299611896499	30.152472318674565	20.778102808477346
82-83	20.792812118835293	27.831126837739124	30.508400251642335	20.867660791783244
84-85	21.376817427249573	28.25441252481798	29.332640674690357	21.036129373242094
86-87	20.347507212036085	29.24828378010925	29.381545824006693	21.02266318384797
88-89	20.803616504581218	28.399024634597836	29.30219551433461	21.495163346486336
90-91	20.767285109639737	27.56180383613763	30.17404235610427	21.496868698118366
92-93	20.781470116570496	28.515510792428483	29.479119777687863	21.223899313313154
94-95	19.835211244950717	28.13119492770974	29.980027150939055	22.053566676400482
96-97	20.02129592751333	28.19574528491669	29.5325836710221	22.250375116547886
98-99	19.630849935133625	28.94065184300507	29.391657467894937	22.036840753966366
100-101	20.054625079379438	27.624135845832516	29.46777802672587	22.853461048062172
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	65.5
2	68.5
3	90.5
4	67.5
5	69.0
6	99.5
7	161.0
8	253.5
9	299.0
10	313.0
11	353.0
12	477.0
13	686.5
14	876.0
15	1074.0
16	1333.0
17	1536.5
18	1676.5
19	1890.5
20	2150.5
21	2386.5
22	2756.0
23	3245.5
24	3822.5
25	4873.5
26	6830.0
27	10842.5
28	19999.0
29	39469.0
30	66354.5
31	93681.5
32	125182.0
33	161788.0
34	206492.0
35	236342.0
36	250192.0
37	269663.0
38	259113.5
39	211630.0
40	168391.5
41	136323.0
42	113032.5
43	98783.5
44	88115.5
45	85100.0
46	85866.5
47	92641.0
48	108340.0
49	106450.0
50	88545.0
51	79111.0
52	73663.5
53	70101.5
54	66457.0
55	60207.5
56	54052.0
57	45909.5
58	38265.5
59	34787.0
60	29822.5
61	23733.5
62	19042.5
63	15529.0
64	12382.5
65	9038.5
66	6831.0
67	5518.5
68	4558.0
69	3787.0
70	3101.5
71	2561.0
72	2170.0
73	1848.0
74	1546.0
75	1305.5
76	1048.0
77	869.0
78	742.5
79	577.5
80	472.5
81	400.0
82	322.5
83	245.5
84	191.0
85	173.0
86	155.5
87	126.0
88	99.0
89	80.5
90	63.5
91	46.0
92	31.0
93	27.5
94	23.5
95	15.0
96	12.5
97	9.0
98	5.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.264830027330321E-4
2	0.004046036059317499
3	3.6544841826093537E-4
4	0.0012007590885716448
5	0.002244897426460032
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.2206916894419345E-5
12-13	1.3051729223604835E-4
14-15	0.0038241566625162172
16-17	0.004855243271180998
18-19	0.0040068808716466845
20-21	0.0
22-23	0.0
24-25	2.6103458447209673E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.3493112602488705E-4
42-43	0.0
44-45	6.395347319566369E-4
46-47	0.7440660313084881
48-49	0.537339692135811
50-51	0.41249990211203086
52-53	0.3477372217045036
54-55	0.33790926959912915
56-57	0.305018911955645
58-59	0.30541046383235315
60-61	0.2812256095810134
62-63	0.2762659524760435
64-65	0.2724678992719745
66-67	0.26009485996799714
68-69	0.23869002404128523
70-71	0.25253790874752996
72-73	0.8222719928163282
74-75	0.5445834018549117
76-77	0.6384644901603014
78-79	0.35907917439981624
80-81	0.31205379400716804
82-83	0.2924370449840899
84-85	0.28313116204765965
86-87	0.25756282449861784
88-89	0.2605908256784942
90-91	0.2708103296605767
92-93	0.22753079555510308
94-95	0.19497978287143264
96-97	0.15049948967738735
98-99	0.159857579530712
100-101	0.15471519821661173
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3830910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.29800765852696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.64437970380384	34.91740970032455
2	10.897618212220069	9.43690313624815
3	3.5333192809494327	4.589570558597085
4	1.593332375140955	2.7595246952572814
5	0.8563798786885126	1.8539771273031806
6	0.49906376487382437	1.296508002816009
7	0.34642371633203983	1.0499619705988024
8	0.220783919061586	0.7647603054726518
9	0.17006416850049458	0.662709570315789
>10	0.9610710597045654	8.033667086567005
>50	0.12543630665156333	3.8142052707297007
>100	0.12319164137264331	10.89883613821874
>500	0.01600841913557169	4.9083208067383675
>1k	0.011598552731202085	9.847087137752958
>5k	8.457278033168187E-4	2.562890684114588
>10k+	4.8327303046675357E-4	2.6036678089452727
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	17403	0.4542784873567899	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	14670	0.38293773542056586	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	12674	0.33083523235993534	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	11413	0.297918771258004	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	11073	0.2890435953859527	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10929	0.2852846973695545	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	10752	0.2806643852243984	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	10630	0.27747976329383883	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	9578	0.2500189250073742	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	9116	0.23795912720476334	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	8413	0.21960839591637493	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7909	0.2064522528589813	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	7666	0.20010911245630933	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7644	0.1995348363704707	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	7228	0.1886757976564315	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	7069	0.18452534776332516	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5991	0.15638581955723313	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5754	0.15019929990524444	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	5548	0.14482198746511926	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	5494	0.14341240070896993	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	5359	0.1398884338185966	No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT	5216	0.13615563926064564	No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA	4544	0.11861411518412075	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4517	0.11790932180604607	No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG	4499	0.1174394595539963	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	4442	0.11595156242250537	No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA	4325	0.11289745778418182	No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA	4279	0.11169669869561018	No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG	4244	0.11078307764995783	No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG	4187	0.1092951805184669	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4155	0.10845986984815618	No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT	4130	0.10780728338697596	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	4107	0.10720690384269012	No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG	4078	0.10644990354772103	No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA	3960	0.10336969545095029	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.220691689441934E-5
2	0.0	0.0	0.0	0.0	5.220691689441934E-5
3	0.0	0.0	0.0	0.0	5.220691689441934E-5
4	0.0	0.0	0.0	0.0	5.220691689441934E-5
5	0.0	7.831037534162901E-5	0.0	0.0	5.220691689441934E-5
6	0.0	7.831037534162901E-5	0.0	0.0	5.220691689441934E-5
7	0.0	7.831037534162901E-5	0.0	0.0	5.220691689441934E-5
8	0.0	7.831037534162901E-5	0.0	0.0	5.220691689441934E-5
9	0.0	7.831037534162901E-5	0.0	2.610345844720967E-5	5.220691689441934E-5
10-11	0.0	7.831037534162901E-5	0.0	5.220691689441934E-5	5.220691689441934E-5
12-13	0.0	7.831037534162901E-5	0.0	5.220691689441934E-5	1.0441383378883868E-4
14-15	0.0	7.831037534162901E-5	0.0	6.525864611802417E-5	1.0441383378883868E-4
16-17	0.0	7.831037534162901E-5	0.0	7.831037534162901E-5	1.0441383378883868E-4
18-19	0.0	7.831037534162901E-5	0.0	7.831037534162901E-5	1.1746556301244351E-4
20-21	0.0	7.831037534162901E-5	0.0	1.1746556301244351E-4	1.3051729223604835E-4
22-23	0.0	7.831037534162901E-5	0.0	2.218793968012822E-4	1.3051729223604835E-4
24-25	1.3051729223604835E-5	7.831037534162901E-5	0.0	4.698622520497741E-4	1.3051729223604835E-4
26-27	2.610345844720967E-5	7.831037534162901E-5	0.0	0.0010832935255592013	1.3051729223604835E-4
28-29	2.610345844720967E-5	7.831037534162901E-5	0.0	0.003028001179876322	1.3051729223604835E-4
30-31	2.610345844720967E-5	7.831037534162901E-5	0.0	0.010859038714039223	1.3051729223604835E-4
32-33	2.610345844720967E-5	7.831037534162901E-5	0.0	0.036048876115596554	1.3051729223604835E-4
34-35	2.610345844720967E-5	7.831037534162901E-5	0.0	0.0777099957973432	1.3051729223604835E-4
36-37	2.610345844720967E-5	7.831037534162901E-5	0.0	0.14101088253182664	1.3051729223604835E-4
38-39	2.610345844720967E-5	7.831037534162901E-5	0.0	0.230506589818085	1.3051729223604835E-4
40-41	2.610345844720967E-5	7.831037534162901E-5	0.0	0.32507941977232563	1.3051729223604835E-4
42-43	2.610345844720967E-5	7.831037534162901E-5	0.0	0.4316467888830591	1.3051729223604835E-4
44-45	2.610345844720967E-5	7.831037534162901E-5	0.0	0.5615114946579273	1.3051729223604835E-4
46-47	2.610345844720967E-5	9.136210456523384E-5	0.0	0.7182888660918685	1.3051729223604835E-4
48-49	2.610345844720967E-5	1.0441383378883868E-4	0.0	0.8769978934509033	1.8272420913046768E-4
50-51	2.610345844720967E-5	1.0441383378883868E-4	0.0	1.0389437496573921	1.8272420913046768E-4
52-53	2.610345844720967E-5	1.0441383378883868E-4	0.0	1.2024297099122663	1.8272420913046768E-4
54-55	2.610345844720967E-5	1.0441383378883868E-4	0.0	1.3806771759190375	2.0882766757767735E-4
56-57	2.610345844720967E-5	1.0441383378883868E-4	0.0	1.5976752259906914	2.0882766757767735E-4
58-59	2.610345844720967E-5	1.0441383378883868E-4	0.0	1.8450838051533447	2.0882766757767735E-4
60-61	2.610345844720967E-5	1.0441383378883868E-4	0.0	2.097778334651558	2.0882766757767735E-4
62-63	2.610345844720967E-5	1.0441383378883868E-4	0.0	2.354049037957039	2.3493112602488705E-4
64-65	2.610345844720967E-5	1.0441383378883868E-4	0.0	2.622196814856	2.3493112602488705E-4
66-67	2.610345844720967E-5	1.0441383378883868E-4	0.0	2.927620330417577	2.3493112602488705E-4
68-69	2.610345844720967E-5	1.0441383378883868E-4	0.0	3.2624493919199358	2.3493112602488705E-4
70-71	2.610345844720967E-5	1.0441383378883868E-4	0.0	3.6201189795636024	2.3493112602488705E-4
72-73	2.610345844720967E-5	1.1746556301244351E-4	0.0	4.005954198871809	2.3493112602488705E-4
74-75	2.610345844720967E-5	1.3051729223604835E-4	0.0	4.379429430605261	2.3493112602488705E-4
76-77	2.610345844720967E-5	1.3051729223604835E-4	0.0	4.792921264138286	2.3493112602488705E-4
78-79	2.610345844720967E-5	1.3051729223604835E-4	0.0	5.257092962246569	2.3493112602488705E-4
80-81	2.610345844720967E-5	1.3051729223604835E-4	0.0	5.778574803375699	2.3493112602488705E-4
82-83	2.610345844720967E-5	1.435690214596532E-4	0.0	6.332907846960644	2.3493112602488705E-4
84-85	2.610345844720967E-5	1.6967247990686284E-4	0.0	6.863121294940367	2.3493112602488705E-4
86-87	3.9155187670814504E-5	1.8272420913046768E-4	0.0	7.43837626047075	2.3493112602488705E-4
88-89	5.220691689441934E-5	1.8272420913046768E-4	0.0	8.04870122242496	2.3493112602488705E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGGG	4775	0.0	57.31778	3
TACCTGG	4960	0.0	55.08804	2
GTACCTG	5385	0.0	51.619713	1
CCTGGGG	4140	0.0	49.067257	4
CTGGGGG	3540	0.0	46.816578	5
GTACCCG	405	0.0	39.754574	1
GTACACG	515	0.0	39.538887	1
TGGGGGG	6950	0.0	34.337097	6
GGGGGGA	7185	0.0	30.577997	7
GGGGAAT	7810	0.0	27.221926	9
TACACGG	745	0.0	26.696615	2
ACACGGG	750	0.0	26.516909	3
ATAACGC	310	0.0	25.967016	3
GGGGGAA	8270	0.0	25.879196	8
CATGGGG	5850	0.0	25.173258	4
TATATCC	5400	0.0	24.465006	4
ATGGGGG	5045	0.0	24.215498	5
TATTCGC	510	0.0	24.139412	9
GTACATG	9285	0.0	23.970613	1
AGGGCTA	5145	0.0	23.744835	5
>>END_MODULE