FastQCFastQC Report
Sat 18 Jun 2016
SRR1931090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830910
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA181190.472968563604992No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC153140.3997483626605689No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142230.37126948949466315No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC124600.3252490922522325No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC115660.30191260040042706No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA113590.29650918450185465No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC110760.2891219057612943No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG108440.2830659034015417No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC104430.2725984165642106No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA85320.22271470747159292No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA84450.22044370658668566No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC81090.21167294454842323No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG77160.20141428537866982No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA75520.19713331819332744No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68030.1775818278163674No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT66720.17416227475978294No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC60150.15701230255996618No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA58370.15236588695636286No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA57260.14946840306872258No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT55510.14490029784046088No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53450.1395229854003357No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA47670.1244351864178485No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG46190.12057187456766148No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA46170.12051966765076706No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA45820.11960604660511472No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC44570.1163431142992135No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA44450.116029872797847No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC42590.11117462952666599No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG42240.11026100848101365No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA40900.10676314504908756No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT40590.10595393783722407No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT39410.1028737297404533No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG39380.10279541936511168No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38930.10162076373498724No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG38770.1012031083998319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG48450.057.644713
TACCTGG50450.056.018542
GTACCTG55050.051.9047431
CTGGGGG31050.050.939965
CCTGGGG39850.050.2990884
GTACCCG3500.044.8266031
GTACACG4600.042.375611
TGGGGGG63600.035.5488666
TACCGGG1102.736515E-630.2260442
TAACGCA3150.030.1575034
GGGGGGA68100.029.921697
TATCACG2053.092282E-1127.8037452
CATGGGG57200.025.8249994
GGGGAAT77700.025.796899
TATTCGC4250.025.7048369
GGGGGAA79200.025.3682868
ACACGGG6600.025.188373
ATGGGGG49850.024.3921875
ATAACGC3350.024.1034973
TACACGG7350.023.9105952
Produced by FastQC (version 0.11.5)