FastQCFastQC Report
Sat 18 Jun 2016
SRR1931089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073258
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54140.5044453430582395No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30230.2816657318184444No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21710.20228127812697413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.189516407052172No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA18990.1769378844602137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.16109826341848837No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA17200.16025969524569114No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17150.1597938240385816No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17000.15839621041725288No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC16010.14917196051648346No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15170.1413453242370427No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC13570.12643744560953657No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA13500.1257852259195832No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC13500.1257852259195832No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12840.11963572598573688No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10910.1016530973913076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17500.063.332942
GTACCTG19450.056.4963231
ACCTGGG20150.055.4713253
GTACCCG2750.051.670231
CCTGGGG17250.049.146834
CTGGGGG16250.042.553095
TGGGGGG30750.031.8828266
GTACACG2100.031.576251
TAACGCA1205.5042165E-627.6279344
GTACTAG6750.027.3660871
GGGGGGA31100.027.25997
GGGGAAT31050.026.084739
GAGTACC4950.024.8782581
TAGTACT9050.024.596934
GCTATTG9900.024.3986958
GGGGGAA34200.024.3735148
CTATTGA10350.023.7965979
TATCACG1401.8191491E-523.6821882
GTACATG39250.022.9279791
CTGTGCG9300.022.918259
Produced by FastQC (version 0.11.5)