FastQCFastQC Report
Sat 18 Jun 2016
SRR1931089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073258
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59620.5555048273574481No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28920.26945990619217375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.2524090200119636No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC23850.2222205657912636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.20945569471646144No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21780.20293349781692754No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA20940.1951068615374868No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA18940.1764720132531041No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC16550.1542033695532668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.15383067258757913No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC16490.1536443241047353No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16490.1536443241047353No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC15290.14246341513410568No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15030.14004088485713592No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA13760.12820775619655292No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13040.12149921081417517No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12840.11963572598573688No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12250.114138445741844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19600.064.703652
ACCTGGG20450.061.5497253
GTACCTG21350.059.6725851
CCTGGGG17900.053.600884
CTGGGGG15100.051.901585
GTACCCG1650.048.978211
ATAACGC1600.038.5919723
TGGGGGG27350.034.3859836
GTATAAC2800.032.2577061
GTACACG1805.456968E-1231.6917821
TAACGCA2150.030.9288064
TATCACG1102.7342103E-630.225882
TCACGCA1153.8656435E-628.9117094
GGGGGGA28400.028.5990337
TAGTACT10300.027.2074854
GGGGAAT30700.026.4564369
TCTAACG2900.026.2056882
CTAACGC2950.025.7615263
CGCCTAC3000.025.3321657
CTGTGCG10400.025.1190369
Produced by FastQC (version 0.11.5)