##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931089_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1073258 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.629625868150995 33.0 33.0 33.0 33.0 33.0 2 32.5853429464304 33.0 33.0 33.0 33.0 33.0 3 32.63718229913031 33.0 33.0 33.0 33.0 33.0 4 36.57628920539143 37.0 37.0 37.0 37.0 37.0 5 36.531294432466375 37.0 37.0 37.0 37.0 37.0 6 36.534023505997624 37.0 37.0 37.0 37.0 37.0 7 36.474665923757385 37.0 37.0 37.0 37.0 37.0 8 36.484608547059516 37.0 37.0 37.0 37.0 37.0 9 36.47650052457098 37.0 37.0 37.0 37.0 37.0 10-11 36.45100246166346 37.0 37.0 37.0 37.0 37.0 12-13 36.360498594000696 37.0 37.0 37.0 37.0 37.0 14-15 38.25574791895332 40.0 37.0 40.0 37.0 40.0 16-17 38.26221095020955 40.0 37.0 40.0 37.0 40.0 18-19 38.234065341232025 40.0 37.0 40.0 37.0 40.0 20-21 38.148098127384095 40.0 37.0 40.0 35.0 40.0 22-23 38.00723265049038 40.0 37.0 40.0 33.0 40.0 24-25 38.16297991722401 40.0 37.0 40.0 37.0 40.0 26-27 38.09384882292981 40.0 37.0 40.0 35.0 40.0 28-29 38.00303282155828 40.0 37.0 40.0 33.0 40.0 30-31 37.880476548975174 40.0 37.0 40.0 33.0 40.0 32-33 37.73400058513424 40.0 37.0 40.0 33.0 40.0 34-35 37.619002141144065 40.0 37.0 40.0 33.0 40.0 36-37 37.550991001231765 40.0 37.0 40.0 33.0 40.0 38-39 37.38503370112312 40.0 37.0 40.0 33.0 40.0 40-41 37.210640405196145 40.0 37.0 40.0 33.0 40.0 42-43 37.014253329581514 40.0 37.0 40.0 33.0 40.0 44-45 36.749741441480055 40.0 37.0 40.0 33.0 40.0 46-47 36.45911421112165 38.5 37.0 40.0 33.0 40.0 48-49 36.16162236852648 37.0 37.0 40.0 33.0 40.0 50-51 35.86323372385763 37.0 37.0 40.0 33.0 40.0 52-53 35.64194163938214 37.0 37.0 40.0 27.0 40.0 54-55 35.39850623056152 37.0 37.0 40.0 27.0 40.0 56-57 35.06290752083842 37.0 35.0 40.0 27.0 40.0 58-59 34.77099541769081 37.0 33.0 40.0 27.0 40.0 60-61 34.48212591939682 37.0 33.0 40.0 27.0 40.0 62-63 34.16816692724396 37.0 33.0 40.0 27.0 40.0 64-65 33.806913621887745 37.0 33.0 37.0 27.0 40.0 66-67 33.44225479800756 37.0 33.0 37.0 27.0 40.0 68-69 33.11529613569151 37.0 33.0 37.0 22.0 40.0 70-71 32.80316708564017 37.0 33.0 37.0 22.0 40.0 72-73 32.44492097892585 37.0 33.0 37.0 22.0 40.0 74-75 32.07292701288973 37.0 33.0 37.0 22.0 37.0 76-77 30.43630142985191 33.0 30.0 37.0 18.5 37.0 78-79 31.220278348728826 33.0 33.0 37.0 22.0 37.0 80-81 31.270035257132953 33.0 33.0 37.0 18.5 37.0 82-83 31.036349135063517 33.0 33.0 37.0 15.0 37.0 84-85 30.73343874445846 33.0 33.0 37.0 15.0 37.0 86-87 30.42333576828684 33.0 33.0 37.0 15.0 37.0 88-89 30.093532962251388 33.0 30.0 37.0 10.5 37.0 90-91 29.655863734535405 33.0 27.0 37.0 6.0 37.0 92-93 29.257075186022373 33.0 27.0 37.0 2.0 37.0 94-95 28.760901386246363 33.0 27.0 37.0 2.0 37.0 96-97 28.262334871950642 33.0 27.0 37.0 2.0 37.0 98-99 27.61411049346942 33.0 27.0 37.0 2.0 37.0 100-101 25.914996673679582 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04509568527141994 1101 2 0.15155425048710214 1101 3 0.1179986558204007 1101 4 0.16543195902994512 1101 5 -0.010196347089056701 1101 6 -0.10181772859476723 1101 7 -0.02824165851729532 1101 8 0.12268769779477395 1101 9 0.04483171361300009 1101 10-11 0.028549820491399203 1101 12-13 0.10183155975300906 1101 14-15 0.16002172916511626 1101 16-17 0.03783904383285375 1101 18-19 -0.0446434702661378 1101 20-21 0.08318319569408317 1101 22-23 -0.01052426190351241 1101 24-25 0.06721284908219616 1101 26-27 -0.012620151158543536 1101 28-29 0.01832757167092325 1101 30-31 0.0432965398718963 1101 32-33 -0.12643737248166786 1101 34-35 0.1176060181402434 1101 36-37 -0.020972613962477737 1101 38-39 0.046116847884434264 1101 40-41 0.12064918518406387 1101 42-43 0.03339337173240864 1101 44-45 -0.11291021901303822 1101 46-47 -0.05866457059852337 1101 48-49 -0.16311320771146143 1101 50-51 -0.4863724524756279 1101 52-53 -0.3653470132231078 1101 54-55 -0.3892827840288149 1101 56-57 -0.5519328533176449 1101 58-59 -0.5408024897845038 1101 60-61 -0.789692241612741 1101 62-63 -0.7270526242589099 1101 64-65 -0.5942063810455807 1101 66-67 -0.8173952488511915 1101 68-69 -0.6606911664085331 1101 70-71 -0.809393256423327 1101 72-73 -1.02819101814082 1101 74-75 -1.2340857203454547 1101 76-77 -1.1432653982156857 1101 78-79 -1.2623417129531838 1101 80-81 -1.3254591469602133 1101 82-83 -1.5505273670466657 1101 84-85 -1.4683949888395524 1101 86-87 -1.802200877288044 1101 88-89 -1.7686004176353691 1101 90-91 -1.6929842478286155 1101 92-93 -1.5620943847724327 1101 94-95 -2.0467236010257643 1101 96-97 -1.9166649525964807 1101 98-99 -1.7429240166235296 1101 100-101 -3.5876332840606047 1102 1 0.08020272524999683 1102 2 0.2072692395391016 1102 3 0.10243175637363322 1102 4 0.10929913185297124 1102 5 0.08955791658731016 1102 6 0.0957246922319257 1102 7 0.08393978040060546 1102 8 0.20442741595378067 1102 9 0.1233734045725825 1102 10-11 0.07799291892845162 1102 12-13 0.18318151159905227 1102 14-15 0.2543117066644811 1102 16-17 0.2824670707299717 1102 18-19 0.26330592705365063 1102 20-21 0.06974651490513395 1102 22-23 0.03359275004644502 1102 24-25 0.19421566490087372 1102 26-27 0.12237989202886723 1102 28-29 0.21018005213942104 1102 30-31 0.04228757416701967 1102 32-33 -0.1698201985789538 1102 34-35 -0.06794703974383509 1102 36-37 -0.12063078566695395 1102 38-39 0.046241011668342935 1102 40-41 0.03189043433651051 1102 42-43 0.15658058048735768 1102 44-45 0.06824420186548252 1102 46-47 0.12378493253039835 1102 48-49 -0.0017645298938049336 1102 50-51 -0.06482722878646996 1102 52-53 -0.1522511890131213 1102 54-55 -0.2205549985492823 1102 56-57 -0.11332968508975227 1102 58-59 -0.079572361388351 1102 60-61 -0.21212388675049 1102 62-63 -0.2206571097025929 1102 64-65 -0.26467947745282316 1102 66-67 -0.4614932583437863 1102 68-69 -0.39655107079162377 1102 70-71 -0.46710285568741483 1102 72-73 -0.4089819090537574 1102 74-75 -0.5867626915265021 1102 76-77 -0.661683745952022 1102 78-79 -0.4009084957939635 1102 80-81 -0.5828663264341039 1102 82-83 -0.8517999918474608 1102 84-85 -0.8794942594043142 1102 86-87 -0.9258447928307589 1102 88-89 -1.1031521755786144 1102 90-91 -0.9881215621767687 1102 92-93 -1.2129863126149196 1102 94-95 -1.3252715541587925 1102 96-97 -1.4843660655358626 1102 98-99 -1.356919326471555 1102 100-101 -1.8643787029568166 1103 1 0.009205134190992226 1103 2 0.17960053612832638 1103 3 0.0673273861617929 1103 4 0.11832800812852184 1103 5 0.1832316033009178 1103 6 0.08724377249205162 1103 7 -0.014509399215413055 1103 8 0.016352190826353308 1103 9 0.10229878943450643 1103 10-11 0.04364379824360043 1103 12-13 0.12750602593380478 1103 14-15 0.17602991226191023 1103 16-17 0.0027316406874788868 1103 18-19 0.013050558630425257 1103 20-21 -0.20603173845756118 1103 22-23 -0.06969188752683664 1103 24-25 0.041714191621515795 1103 26-27 -0.014132106151507173 1103 28-29 -0.09658100779461876 1103 30-31 -0.1984276641940781 1103 32-33 -0.06090866771246084 1103 34-35 -0.1387326853503552 1103 36-37 -0.0024903226953725266 1103 38-39 0.1070745259307202 1103 40-41 -0.09076239901233407 1103 42-43 -0.09421499036962189 1103 44-45 -0.06092517873685921 1103 46-47 -0.21423291224282082 1103 48-49 -0.20129566519771203 1103 50-51 -0.318340870836451 1103 52-53 -0.42295153949852704 1103 54-55 -0.20263785371300003 1103 56-57 -0.25797108372296407 1103 58-59 -0.04306243096849727 1103 60-61 -0.31853446127774987 1103 62-63 -0.4242684534367598 1103 64-65 -0.3740459156319531 1103 66-67 -0.26490196154777834 1103 68-69 -0.5091856044599368 1103 70-71 -0.4474446565000463 1103 72-73 -0.5652642824292755 1103 74-75 -0.6860100267001421 1103 76-77 -0.8128153278921992 1103 78-79 -0.5209083924059392 1103 80-81 -0.5826673044848292 1103 82-83 -0.5963622986410932 1103 84-85 -0.5387728177616857 1103 86-87 -0.4020826059672373 1103 88-89 -0.6091610876264184 1103 90-91 -0.6651425396071637 1103 92-93 -0.721923690487813 1103 94-95 -1.0104622533720082 1103 96-97 -1.197648842124675 1103 98-99 -1.0888900056274942 1103 100-101 -1.5188409301416677 1104 1 -0.018244803785258057 1104 2 0.1252322922863982 1104 3 0.0841446819264533 1104 4 0.09647792838370606 1104 5 0.08941625685398691 1104 6 0.048310580927285685 1104 7 0.07113653379894203 1104 8 0.1267899012640612 1104 9 0.06524403132711853 1104 10-11 0.11073577059873685 1104 12-13 0.07467065467737655 1104 14-15 0.20033436025056517 1104 16-17 0.19958615069987928 1104 18-19 0.07816663164122417 1104 20-21 0.2116716108247303 1104 22-23 0.23794589379515685 1104 24-25 0.21150862720542563 1104 26-27 0.24239810827344854 1104 28-29 0.29786343664982695 1104 30-31 0.2529191371527233 1104 32-33 0.3458341995567267 1104 34-35 0.3267289485323275 1104 36-37 0.22511024437887528 1104 38-39 0.24091290976910074 1104 40-41 0.314137476940175 1104 42-43 0.23454630681272448 1104 44-45 0.44007606173806835 1104 46-47 0.28334816165404675 1104 48-49 0.22857319888167638 1104 50-51 0.3279642859950229 1104 52-53 0.30098962654790284 1104 54-55 0.33205114150231196 1104 56-57 0.4130195241097496 1104 58-59 0.47122961227091764 1104 60-61 0.09185894648408066 1104 62-63 0.1116875983087624 1104 64-65 0.28841199682596397 1104 66-67 0.445774983339696 1104 68-69 0.17902988375555395 1104 70-71 0.14817823912284922 1104 72-73 0.22276513454750813 1104 74-75 0.01538993785771936 1104 76-77 0.20209695280955486 1104 78-79 0.261919890422341 1104 80-81 0.1566114479518177 1104 82-83 0.2679917683445545 1104 84-85 0.35793992368789773 1104 86-87 0.29916672949262235 1104 88-89 0.28521247253661386 1104 90-91 0.2309676854504339 1104 92-93 0.35081244277463597 1104 94-95 0.36384219461664813 1104 96-97 0.120196275720442 1104 98-99 0.25093809999008343 1104 100-101 -0.5935706838187897 1105 1 0.012499762719265561 1105 2 0.1209674765606863 1105 3 0.059652506524294324 1105 4 0.06834216842305807 1105 5 -0.029238896085473698 1105 6 0.07697283885663353 1105 7 0.07328442689617276 1105 8 0.04108550527929822 1105 9 0.10911461120360144 1105 10-11 0.05451890444581409 1105 12-13 0.12343730130432817 1105 14-15 0.19162983674033285 1105 16-17 0.05703637022585184 1105 18-19 0.20741004130765361 1105 20-21 0.1322624180608969 1105 22-23 9.963027875201647E-4 1105 24-25 -0.006345848027315526 1105 26-27 0.11816280558265646 1105 28-29 0.23415627154079033 1105 30-31 0.029156589558404278 1105 32-33 -0.07582417795507013 1105 34-35 -0.19664828999493267 1105 36-37 -0.18158028776664992 1105 38-39 -0.11900401650296288 1105 40-41 0.006646267087916158 1105 42-43 -0.06252425120224814 1105 44-45 -0.19186542229392245 1105 46-47 -0.19503627295271997 1105 48-49 -0.019581325539448358 1105 50-51 -0.34609976822151367 1105 52-53 -0.2872534369779913 1105 54-55 -0.22165984937050354 1105 56-57 0.03784690929128942 1105 58-59 0.13458790415847233 1105 60-61 -0.05646045013831724 1105 62-63 -0.1981191095304098 1105 64-65 -0.17865273145371674 1105 66-67 0.21044496566830873 1105 68-69 0.170324449349188 1105 70-71 0.018981285159547667 1105 72-73 0.006839937338497748 1105 74-75 0.3074715177159817 1105 76-77 0.14642662434040687 1105 78-79 0.08076724244726563 1105 80-81 0.28825689073843463 1105 82-83 0.27103598324317346 1105 84-85 0.33371372995850734 1105 86-87 0.275397805625321 1105 88-89 0.3510720852235707 1105 90-91 0.01753470007942326 1105 92-93 -0.16937483747166837 1105 94-95 -0.2266380298281625 1105 96-97 0.039182083958589686 1105 98-99 -0.18190426032399287 1105 100-101 -0.7640075496073528 1106 1 0.1345569179242787 1106 2 0.23543635092367765 1106 3 0.1566074702395639 1106 4 0.06737337509296992 1106 5 0.07147239269777117 1106 6 0.1261706131530005 1106 7 0.08584092483432926 1106 8 0.10313329668260707 1106 9 0.16489585838995424 1106 10-11 0.09112146508542907 1106 12-13 0.10813018661576734 1106 14-15 0.12729691757515127 1106 16-17 0.11878524026708703 1106 18-19 0.2828759804789627 1106 20-21 0.15002302886887975 1106 22-23 0.14820967141518793 1106 24-25 0.25119921526838596 1106 26-27 0.22713314754149394 1106 28-29 0.21693858115850162 1106 30-31 0.14946163340620444 1106 32-33 0.09605110340915957 1106 34-35 0.19837538961898815 1106 36-37 0.2316370784840487 1106 38-39 0.14826679544668764 1106 40-41 0.042413288498607926 1106 42-43 0.21719681555084946 1106 44-45 0.21078615424615776 1106 46-47 0.2901257028094406 1106 48-49 0.13305944661405533 1106 50-51 -0.036087703323168796 1106 52-53 -0.1305448132767708 1106 54-55 0.04523100831771387 1106 56-57 0.025184168218416403 1106 58-59 -0.10226135664140656 1106 60-61 0.03334561112255585 1106 62-63 0.1977815261276632 1106 64-65 0.3058600807480403 1106 66-67 0.1858424770579319 1106 68-69 0.1465421937534117 1106 70-71 0.2150268796709156 1106 72-73 0.1155554758471311 1106 74-75 0.3429063042393068 1106 76-77 0.5005115828335569 1106 78-79 0.3635576553549065 1106 80-81 0.4239158682833164 1106 82-83 0.5349187196341454 1106 84-85 0.4790849506741175 1106 86-87 0.5715613480235362 1106 88-89 0.35921048941567335 1106 90-91 0.547650126942731 1106 92-93 0.5380342706262162 1106 94-95 0.7281843280491351 1106 96-97 0.9889074724663196 1106 98-99 0.6027138946469286 1106 100-101 0.32181709519843693 1107 1 0.05648314422824541 1107 2 0.14909166017310582 1107 3 0.038379658205947464 1107 4 0.05068038673879016 1107 5 0.09595851169959957 1107 6 0.06123162213364708 1107 7 0.10215863368345879 1107 8 0.031876231959053314 1107 9 0.12531342633553066 1107 10-11 0.1112354041129393 1107 12-13 0.09230459776573952 1107 14-15 0.060383365641513365 1107 16-17 0.024554467695153903 1107 18-19 0.1532364572924223 1107 20-21 0.0822177537594726 1107 22-23 0.13266076432987717 1107 24-25 0.12295309098484353 1107 26-27 0.08004182431638185 1107 28-29 0.13224876878086889 1107 30-31 0.12256312740085917 1107 32-33 0.186846157364279 1107 34-35 0.11064294653203888 1107 36-37 0.20232792288030765 1107 38-39 0.40094196497398826 1107 40-41 0.27418964623727504 1107 42-43 0.3141053637577613 1107 44-45 0.3604440591788247 1107 46-47 0.4984001815444685 1107 48-49 0.48680946181634255 1107 50-51 0.4352655267735557 1107 52-53 0.23193957179474722 1107 54-55 0.3741535044128952 1107 56-57 0.4044106233386344 1107 58-59 0.45972969224743565 1107 60-61 0.49258348099438365 1107 62-63 0.38294069849736445 1107 64-65 0.700361367296523 1107 66-67 0.79085124226674 1107 68-69 0.7788612299800448 1107 70-71 0.8520990219435376 1107 72-73 0.8789087372171451 1107 74-75 0.9795727624173622 1107 76-77 0.9400451069339688 1107 78-79 1.0639186881380027 1107 80-81 1.019201245760641 1107 82-83 0.948997521380587 1107 84-85 1.3491578869408833 1107 86-87 1.2269083860084251 1107 88-89 1.0931268959059253 1107 90-91 1.3886141435453716 1107 92-93 1.3624151897482726 1107 94-95 1.6301130214683681 1107 96-97 1.6484082117321996 1107 98-99 2.000892399123348 1107 100-101 2.4991418735489823 1108 1 0.08580578638219549 1108 2 0.08592360938053645 1108 3 0.04158969428236503 1108 4 0.04918778701979676 1108 5 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0.07429662456369357 2316 70-71 0.1908601384926456 2316 72-73 0.18422974795647207 2316 74-75 0.23670667627133923 2316 76-77 0.2634459197066299 2316 78-79 0.2001212856301109 2316 80-81 0.287088028646302 2316 82-83 0.39008175977749104 2316 84-85 0.2974033203252766 2316 86-87 0.3922763613216631 2316 88-89 0.4681534591148768 2316 90-91 0.3974671790535105 2316 92-93 0.2816622140535081 2316 94-95 0.5545959721957701 2316 96-97 0.8213808542935546 2316 98-99 1.3338891967695439 2316 100-101 1.85222045716176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 26.0 6 36.0 7 99.0 8 245.0 9 523.0 10 909.0 11 1361.0 12 2174.0 13 3178.0 14 3684.0 15 4544.0 16 5676.0 17 7726.0 18 7763.0 19 6216.0 20 5447.0 21 5443.0 22 5798.0 23 6471.0 24 7247.0 25 8613.0 26 10271.0 27 12523.0 28 15425.0 29 19186.0 30 24078.0 31 30772.0 32 40587.0 33 54272.0 34 77999.0 35 119159.0 36 191218.0 37 280904.0 38 113665.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4397633334732 21.35798842777148 13.490924519416819 24.71132371933849 2 14.035221759559919 22.079520562176626 39.75962886801577 24.125628810247683 3 17.93706639037398 27.787447193498675 30.05446966153525 24.221016754592092 4 11.723462578429418 17.723324680552114 38.854497241110714 31.69871549990776 5 12.301981443418079 38.13966446092179 34.85648371593783 14.701870379722305 6 28.850378939639864 38.34064129966886 18.94623659921473 13.862743161476551 7 26.416667753699485 31.91497291424802 23.875992538606745 17.79236679344575 8 26.017882000413696 34.20976130622832 20.444292052796254 19.328064640561728 9 26.19444718790822 15.429468030986026 22.012787232892745 36.36329754821301 10-11 25.497084577985905 25.843087123506187 28.44418583416103 20.215642464346875 12-13 25.91450517955608 24.258006928436593 28.10866539080072 21.718822501206606 14-15 22.425554772372468 25.714659743183237 26.932403328005307 24.927382156438984 16-17 21.34351665675914 27.498141173883635 28.012462986532594 23.145879182824633 18-19 21.02392900868198 28.156510363770877 29.42847852054212 21.391082107005026 20-21 22.338477793783042 26.97753009993869 28.630907013970546 22.053085092307718 22-23 22.190982969612154 27.017082565422296 28.3832498802711 22.408684584694456 24-25 22.24786584400023 26.955867088808095 29.15855274314284 21.637714324048833 26-27 22.182737049246313 26.6905534363592 29.007284362194362 22.119425152200122 28-29 21.26795234696597 27.932985358599705 28.9976408282072 21.801421466227133 30-31 22.33018528629649 26.97100790303916 28.639618805543492 22.059188005120856 32-33 22.645347157906116 27.244846998578158 28.270928332236984 21.838877511278742 34-35 22.618326627893758 26.85230391946764 28.98464302152884 21.544726431109762 36-37 22.48699753460957 26.514687055675335 29.06249010023685 21.935825309478243 38-39 22.238662314804376 27.083308098619437 29.15774134247436 21.52028824410183 40-41 22.016342325075204 26.59338601253884 29.151049802674272 22.239221859711684 42-43 21.95559328303796 26.93843188576897 28.276214867123215 22.829759964069854 44-45 22.116117466629646 27.206692146715888 28.267434298183662 22.409756088470807 46-47 22.49808526933878 27.194672669572462 28.069998080610624 22.237243980478134 48-49 22.879898700823148 26.80799044776342 28.24357457886349 22.068536272549945 50-51 21.80272590560704 27.301962808569797 28.285277165415955 22.610034120407207 52-53 22.037245471265994 27.73219486833548 27.48118346194485 22.74937619845368 54-55 21.921895760385667 27.285424380717405 27.6778742855865 23.114805573310424 56-57 23.105115452202547 26.62924478550358 27.53163731367481 22.734002448619066 58-59 22.76880302779015 27.03692867884516 27.80468442816173 22.38958386520296 60-61 22.510570617689314 27.715842788965933 27.691757247558368 22.08182934578638 62-63 22.396944630275293 27.108160386412216 27.464179162885344 23.030715820427147 64-65 21.578036222418095 27.947287604657966 27.48910327246571 22.98557290045823 66-67 22.283936209416336 26.921010910517357 27.112535471177907 23.682517408888398 68-69 21.772406200453883 27.186365375037447 27.376082006784515 23.665146417724156 70-71 22.20345773798154 26.89469785556886 27.4513275160844 23.4505168903652 72-73 22.561350579264257 26.58601193748381 28.061426050399813 22.79121143285212 74-75 22.231850231654565 27.832882602730475 27.240853196926178 22.69441396868878 76-77 22.411152046424 26.94523488732229 27.191638760461334 23.451974305792376 78-79 22.08425464429244 27.235256462221823 27.522015954170666 23.158472939315075 80-81 21.93353494025636 27.23510439005209 27.868158203819288 22.96320246587226 82-83 22.325013009968643 26.34424307140551 28.799807680750046 22.530936237875803 84-85 22.23450600779609 27.199562442550786 27.596541469889274 22.969390079763855 86-87 21.482183396389207 27.785953844125512 27.624389182621645 23.107473576863644 88-89 21.950080968415794 26.98973592556496 27.3644827245639 23.695700381455346 90-91 22.034693444956126 26.534266007924472 27.634188440332352 23.796852106787046 92-93 22.456490657085766 26.99407824763463 27.587884050868087 22.96154704441151 94-95 21.24258188232203 26.9659817166519 28.123948837933057 23.66748756309302 96-97 20.867436313151078 27.296127541417412 28.044809079219547 23.79162706621196 98-99 20.909930324302266 27.9095986240028 27.144265404963207 24.036205646731727 100-101 19.41619706761481 27.97915874684139 27.40301475137339 25.201629434170414 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97.0 1 70.5 2 73.5 3 93.5 4 65.5 5 68.5 6 122.5 7 211.5 8 323.0 9 415.5 10 427.5 11 442.0 12 505.5 13 581.0 14 664.0 15 754.5 16 877.0 17 1009.5 18 1168.0 19 1300.0 20 1411.0 21 1579.5 22 1834.5 23 2200.0 24 2581.5 25 3123.5 26 4062.5 27 5110.0 28 6396.0 29 8643.0 30 11772.0 31 14484.0 32 17725.5 33 21240.5 34 23982.5 35 26732.5 36 29164.5 37 33031.5 38 34658.5 39 33106.5 40 32153.5 41 31147.0 42 31075.5 43 32040.0 44 33904.0 45 38097.0 46 42229.5 47 47462.5 48 56346.0 49 55344.0 50 46598.0 51 42667.0 52 40200.0 53 37904.5 54 35948.0 55 32478.5 56 28156.5 57 23409.0 58 19298.0 59 17692.5 60 14926.0 61 10954.0 62 8017.0 63 6310.0 64 4821.0 65 3045.5 66 2020.5 67 1457.5 68 1197.5 69 951.0 70 714.5 71 539.0 72 427.5 73 364.5 74 288.0 75 228.5 76 189.0 77 133.0 78 94.5 79 80.0 80 60.0 81 46.0 82 36.0 83 30.0 84 26.0 85 24.5 86 19.5 87 10.5 88 6.5 89 7.0 90 8.5 91 5.5 92 2.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08040937034711132 2 9.317424142191347E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3293560355478368E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.317424142191347E-5 40-41 0.0030281628462121876 42-43 0.0060097385717134185 44-45 0.0 46-47 0.0 48-49 1.3976136213287018E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.00256229163910262 68-69 0.007314177951620207 70-71 0.001211265138484875 72-73 0.0 74-75 4.658712071095673E-5 76-77 0.0018634848284382695 78-79 0.005497280243892895 80-81 0.00442577646754089 82-83 0.003959905260431322 84-85 0.00256229163910262 86-87 3.261098449766971E-4 88-89 0.0 90-91 4.658712071095673E-5 92-93 0.001071503776352005 94-95 0.003820143898298452 96-97 0.0035406211740327113 98-99 0.0 100-101 1.8634848284382693E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1073258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.20783913159004 #Duplication Level Percentage of deduplicated Percentage of total 1 88.8587892073837 47.27984161574341 2 6.723824179398749 7.15520310573088 3 1.4488020551015357 2.3126288004407867 4 0.6465868800893712 1.3761396280156784 5 0.3735186837792586 0.9937061019585022 6 0.2524631718286842 0.8059811899986962 7 0.18792258823961142 0.6999268390971696 8 0.14083388376640388 0.5994773305375894 9 0.1107744400707032 0.5304661728456543 >10 0.9355339170550796 10.325102462855352 >50 0.16012861602091746 6.003594661800653 >100 0.14762579278734042 14.727795832077886 >500 0.009853449744150502 3.614635036715969 >1k 0.0031671802749055184 3.017329446458571 >5k 1.7595445971697326E-4 0.5581717757232927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5962 0.5555048273574481 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2892 0.26945990619217375 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.2524090200119636 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 2385 0.2222205657912636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2248 0.20945569471646144 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2178 0.20293349781692754 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 2094 0.1951068615374868 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 1894 0.1764720132531041 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 1655 0.1542033695532668 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.15383067258757913 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 1649 0.1536443241047353 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 1649 0.1536443241047353 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 1529 0.14246341513410568 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1503 0.14004088485713592 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1376 0.12820775619655292 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1304 0.12149921081417517 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1284 0.11963572598573688 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1225 0.114138445741844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 9.317424142191347E-5 20-21 0.0 0.0 0.0 9.317424142191347E-5 9.317424142191347E-5 22-23 0.0 0.0 0.0 2.3293560355478366E-4 9.317424142191347E-5 24-25 0.0 0.0 0.0 4.658712071095673E-4 9.317424142191347E-5 26-27 0.0 0.0 0.0 7.453939313753076E-4 9.317424142191347E-5 28-29 0.0 0.0 0.0 0.001537374983461572 9.317424142191347E-5 30-31 0.0 0.0 0.0 0.00670854538237777 9.317424142191347E-5 32-33 0.0 0.0 0.0 0.022454992182681145 9.317424142191347E-5 34-35 0.0 9.317424142191347E-5 0.0 0.04784497297015256 9.317424142191347E-5 36-37 0.0 9.317424142191347E-5 0.0 0.08725767709162197 9.317424142191347E-5 38-39 0.0 9.317424142191347E-5 0.0 0.13976136213287021 9.317424142191347E-5 40-41 0.0 9.317424142191347E-5 0.0 0.1951068615374868 9.317424142191347E-5 42-43 0.0 9.317424142191347E-5 0.0 0.24938085716575137 9.317424142191347E-5 44-45 0.0 9.317424142191347E-5 0.0 0.31777075036943586 9.317424142191347E-5 46-47 0.0 9.317424142191347E-5 0.0 0.40162756764915797 9.317424142191347E-5 48-49 0.0 9.317424142191347E-5 0.0 0.48855913489580327 9.317424142191347E-5 50-51 0.0 9.317424142191347E-5 0.0 0.5780064066608401 9.317424142191347E-5 52-53 0.0 9.317424142191347E-5 0.0 0.6656367807181498 9.317424142191347E-5 54-55 0.0 9.317424142191347E-5 0.0 0.7591837191057509 9.317424142191347E-5 56-57 0.0 9.317424142191347E-5 0.0 0.8741607330203921 9.317424142191347E-5 58-59 0.0 9.317424142191347E-5 0.0 1.012198371686957 9.317424142191347E-5 60-61 0.0 9.317424142191347E-5 0.0 1.1550344837867503 9.317424142191347E-5 62-63 0.0 9.317424142191347E-5 0.0 1.2993613837492943 9.317424142191347E-5 64-65 0.0 9.317424142191347E-5 0.0 1.453471579061139 9.317424142191347E-5 66-67 0.0 9.317424142191347E-5 0.0 1.625843925691679 9.317424142191347E-5 68-69 0.0 1.397613621328702E-4 0.0 1.8220688781262289 9.317424142191347E-5 70-71 0.0 2.3293560355478366E-4 0.0 2.0345061485681915 9.317424142191347E-5 72-73 0.0 2.795227242657404E-4 0.0 2.24722294173442 9.317424142191347E-5 74-75 0.0 2.795227242657404E-4 0.0 2.4644120984889 9.317424142191347E-5 76-77 0.0 2.795227242657404E-4 0.0 2.7081559140486258 9.317424142191347E-5 78-79 0.0 2.795227242657404E-4 0.0 2.9915919564540863 9.317424142191347E-5 80-81 0.0 2.795227242657404E-4 0.0 3.289330244917811 9.317424142191347E-5 82-83 0.0 2.795227242657404E-4 0.0 3.5994141203699392 9.317424142191347E-5 84-85 0.0 2.795227242657404E-4 0.0 3.9080072079593164 9.317424142191347E-5 86-87 0.0 3.7269696568765386E-4 0.0 4.245810420234464 9.317424142191347E-5 88-89 0.0 3.7269696568765386E-4 0.0 4.5997327762756015 9.317424142191347E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1960 0.0 64.70365 2 ACCTGGG 2045 0.0 61.549725 3 GTACCTG 2135 0.0 59.672585 1 CCTGGGG 1790 0.0 53.60088 4 CTGGGGG 1510 0.0 51.90158 5 GTACCCG 165 0.0 48.97821 1 ATAACGC 160 0.0 38.591972 3 TGGGGGG 2735 0.0 34.385983 6 GTATAAC 280 0.0 32.257706 1 GTACACG 180 5.456968E-12 31.691782 1 TAACGCA 215 0.0 30.928806 4 TATCACG 110 2.7342103E-6 30.22588 2 TCACGCA 115 3.8656435E-6 28.911709 4 GGGGGGA 2840 0.0 28.599033 7 TAGTACT 1030 0.0 27.207485 4 GGGGAAT 3070 0.0 26.456436 9 TCTAACG 290 0.0 26.205688 2 CTAACGC 295 0.0 25.761526 3 CGCCTAC 300 0.0 25.332165 7 CTGTGCG 1040 0.0 25.119036 9 >>END_MODULE